Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008602

Physical Location & Seq

Genomic Coordinates Forward (+)

30041883 .. 30046238

Gene Structure 4356 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1205000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGGGGAGCGTTGGAGAGGTCGGAGAAGGGCGGGTGTTGTCGGATGTGGAGGAGACTATATTTGTTGCTGTGGGGAAGGACGTGAAGCAGAGCGAAACGACGCTGGCTTGGGCGGTGAAGAACTTCGCCGGGAAGAGGATTTGCCTGCTACATGTTCACAAACCTTCTCTTCTACTGTCGATGTCAGAAGGGATCGGTTCTGCTAGTAAATTGAAACAAGGAGCAGTTAAGGTGTTACAGGTAGCTGAGAGGACGAAATTACGAAAGCTTCTAGATCAATACCGAATGATTCTCACCGGAGCGGGGGTGGAGGCAAATGAATTATGGATGGAGATGGGAAATGTCGAAGAGGGGATTGTCGAAATAGTTGCTCGGCACCATATTAGATGGCTTGTCATGGGGGCAGCTGCAGATGAACATTATTCTGAGAGATTGGCTGAGATAAAGTCCAGGAAAGCATTTTTCGTATGCCAAAAGGCAGCTAGTTCCTGTCATATTTGGTTTGTTTGTAAGGGGTACCTCATATATACAAGGGGAGACAGAGAAGATGAATCTGAAATAGAAATTGCCCCTCCTCTGCTGCTGACGAATTGGGCTTTTGGAACAGAGCAACCAGAACACTTCAGATCAGAATCAACTGATTCGCTGAGAAATCTGAGCTCTGCAGATGCTGAGGAAGATACCAGCGAGTTGGAAGAAATATCAGGGAGGTCGACCCCGTTGATGATAGATGCGGAGGGAGAAGAAAATGGTGAAAGTTATCATAGCGTAGAACAAGCCATTGTGGATGCTAAGAACTTAAAACTGAAGGAATTTGAAGAGACAACTAGGCGGTGGAAAGAGGAAGATGATGCGGTGGAGGCTAAATGCAAGGCCAAAGCATTTGAAAGTTTATGCACTAAGGAGATGAGCCAAAGAAAAGAGATGGAAGAAATGCTTGCTAGATTAAAGCAAGAAATAGACAGGGTGAAGGATGAGAGTGAGGAATCTATAAGACAACTCCCAATGGTTGAGGACAAGAAGTTAGTGCTGGAGAAACAACTAGAAGCGTCACAAAGTGAGGTCAAGGAGTTAGAGGAGAAAATCATCTCAGCTGCGGGGCTCTTGATAAGTTTCAGGGAAAAACGGGATAAAATGAAGATAGAGCATAGAAATGCAATGAGAAAAGTCAGACAGCTAGAGACAATGATAAATGGGGAAGCTGCAAGCTTTGATCAGGCAGAATTCCCTATCTTCTCTTTTATGGAGATCAACGAGGCAACTCACAACTTTGATCCGTCCTGGAAGATTAAAGAAGGAAGGCGCGGAAGTGTTTATAAAGGGATTCTTCGCCACATGCATGTTGCTATTAAGATGTTGCCTTCTTATGGTTCTAAAACTCAATTGGACTTCGAACATGAGGTAGAGGTCTTGAGCAGAGTGCGGCATCCAAACTTAGTAACACTAATTGGAACCTGTCCAGAATCCAGGTCAGTTGTATATGAGTACCTAAAGAATGGCTGCCTTGAAGACCTCCTTGCCTGCAAAGACAACACTCCGCCTCTTCCATGGCAAATTCGAACGTCCATTGCTACAGAGATATGCTCTGCCCTCATATTCCTTCACTCAAATATCCCCTGTCTTGTCCATGGGAACGTGAAACCCAGTAACATTCTCTTTGAAGCGAACTTTGTGAGCAAACTTATAAATTTGGGCATCAACTCTTTGATCCCACAGAACGAAAACCCGACCAACTTCACCAAAATACGTAGTGACCCAAATGGAGCTCATGTGTATATGGATCCAGAGTATCTTGAAACTGGAAAACTCACACCAGAATCAGATGTTTACTCATTCGGGATTATACTGTTGCAACTTTTAACTGCAAGTCCGCTTTCAGGAATAGTGAAGGATGTGAAATATGCATTAGAAAATGACAACTTCAAAGCATTGCTGGACGTTTCAGCCGGAGATTGGCCACTAGAGCAAGCAAAAGAGATGGCTTACATAGCACTGAGGTGCTGCGAGAACAAGCGCTTGGATCGGCTAGACCTATTGGTTGCCCACGAGGCAATGAGAGCTTCATACGCTGCCTCAGCAACGTGCTTGGTTTCCAAGAAGCTTATTCGGACACCATCCCATTTCGTCTGCCCCATTCTGCAGGAAGTCATGCAAGATCCACACATTGCAGCAGATGGTTTCACATACGAAGAAGAAGCAATACGGGGATGGCTGAAAAGCGGCCACAACACGTCGCCGATGACAAATCTTGAGCTCGAACACTGCAATCTGGTCCCCAATTATGCCCTCCAGTATGCAATTCAACAGTGGCAACTACAGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

775

Amino Acids

87.5

Weight (kDa)

5.42

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	429 - 676	3.5e-32	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	429 - 686	2.2e-30	Protein kinase domain
U-box	PF04564	705 - 771	3.2e-16	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49060 AT3G49060 AT3G49060
fragaria_vesca	FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840
malus_domestica	MD01G1205000.v1.1
prunus_persica	Prupe.2G297400_v2.0.a1
pyrus_communis	pycom01g21460
rosa_chinensis	RchiOBHm_Chr1g0377811
rosa_laevigata	RLG00000026496
rosa_multiflora	Rmu_co8492821.1_g000001 Rmu_sc0004824.1_g000003
rosa_roxburghii	Rroxscaffold_4G00281040
rosa_rugosa	Rorug01G0405200
rosa_samantha	Rh1AG427100 Rh1BG382200 Rh1CG396100 Rh1DG413200
rosa_wichuraiana	Rw1G037150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1317, 1685
Acc65I	GGTACC	1 cut(s)	516
AccB1I	GGYRCC	2 cut(s)	375, 516
AccBSI	CCGCTC	1 cut(s)	302
AccI	GTMKAC	1 cut(s)	713
AccII	CGCG	1 cut(s)	1305
AclWI	GGATC	7 cut(s)	200, 1268, 1702, 1775, 1788, 2028, 2150
AcoI	YGGCCR	2 cut(s)	1955, 2221
AcsI	RAATTY	4 cut(s)	812, 1223, 1554, 1687
AcuI	CTGAAG	2 cut(s)	607, 827
AdeI	CACNNNGTG	1 cut(s)	1058
AfaI	GTAC	2 cut(s)	518, 1487
AfeI	AGCGCT	1 cut(s)	2015
AfiI	CCNNNNNNNGG	5 cut(s)	135, 303, 1367, 1877, 1953
AflIII	ACRYGT	2 cut(s)	151, 2229
AgsI	TTSAA	8 cut(s)	214, 818, 887, 1508, 1661, 1796, 1924, 2303
AjiI	CACGTC	2 cut(s)	82, 2232
AjnI	CCWGG	3 cut(s)	449, 1280, 1466
AjuI	GAANNNNNNNTTGG	2 cut(s)	2000, 2032
AleI	CACNNNNGTG	1 cut(s)	1996
Alw21I	GWGCWC	3 cut(s)	662, 1768, 2256
Alw26I	GTCTC	4 cut(s)	47, 531, 815, 1175
AlwI	GGATC	7 cut(s)	200, 1268, 1702, 1775, 1788, 2028, 2150
AlwNI	CAGNNNCTG	1 cut(s)	671
Aor51HI	AGCGCT	1 cut(s)	2015
AoxI	GGCC	3 cut(s)	873, 1955, 2221
ApoI	RAATTY	4 cut(s)	812, 1223, 1554, 1687
Asp700I	GAANNNNTTC	1 cut(s)	122
Asp718I	GGTACC	1 cut(s)	516
AspLEI	GCGC	2 cut(s)	1305, 2016
AspS9I	GGNCC	1 cut(s)	2272
AsuC2I	CCSGG	1 cut(s)	130
AsuHPI	GGTGA	5 cut(s)	127, 286, 764, 979, 1729
AsuII	TTCGAA	2 cut(s)	1392, 1558
AvaII	GGWCC	1 cut(s)	2272
BalI	TGGCCA	1 cut(s)	1957
BamHI	GGATCC	1 cut(s)	1780
BanI	GGYRCC	2 cut(s)	375, 516
BanII	GRGCYC	4 cut(s)	662, 1104, 1768, 2256
BarI	GAAGNNNNNNTAC	2 cut(s)	2184, 2216
BauI	CACGAG	1 cut(s)	2045
BbsI	GAAGAC	1 cut(s)	1515
Bbv12I	GWGCWC	3 cut(s)	662, 1768, 2256
BbvCI	CCTCAGC	2 cut(s)	672, 2074
BccI	CCATC	8 cut(s)	322, 328, 381, 919, 1972, 2122, 2168, 2202
BcgI	CGANNNNNNTGC	2 cut(s)	350, 384
BciT130I	CCWGG	3 cut(s)	451, 1282, 1468
BclI	TGATCA	1 cut(s)	1213
BcnI	CCSGG	1 cut(s)	130
BcoDI	GTCTC	4 cut(s)	47, 531, 815, 1175
BfaI	CTAG	9 cut(s)	204, 272, 483, 828, 942, 1043, 1178, 1961, 2027
BfmI	CTRYAG	5 cut(s)	408, 663, 1572, 2138, 2315
BfoI	RGCGCY	1 cut(s)	2017
BglI	GCCNNNNNGGC	1 cut(s)	2048
Bme1390I	CCNGG	4 cut(s)	130, 451, 1282, 1468
Bme18I	GGWCC	1 cut(s)	2272
BmgBI	CACGTC	2 cut(s)	82, 2232
BmgT120I	GGNCC	1 cut(s)	2272
BmiI	GGNNCC	5 cut(s)	377, 518, 1453, 1782, 2274
BmrFI	CCNGG	4 cut(s)	130, 451, 1282, 1468
BmrI	ACTGGG	1 cut(s)	1638
BmsI	GCATC	6 cut(s)	658, 721, 778, 841, 1435, 1704
BmuI	ACTGGG	1 cut(s)	1638
BpiI	GAAGAC	1 cut(s)	1515
BplI	GAGNNNNNCTC	2 cut(s)	1568, 1600
BpmI	CTGGAG	2 cut(s)	1052, 2273
Bpu10I	CCTNAGC	2 cut(s)	672, 2074
Bpu14I	TTCGAA	2 cut(s)	1392, 1558
BpuEI	CTTGAG	2 cut(s)	1432, 2270
BpuMI	CCSGG	1 cut(s)	130
BsaAI	YACGTR	1 cut(s)	1748
BsaBI	GATNNNNATC	1 cut(s)	631
BsaJI	CCNNGG	2 cut(s)	1547, 1627
BsaWI	WCCGGW	1 cut(s)	296
BsaXI	ACNNNNNCTCC	4 cut(s)	291, 321, 1755, 1785
Bsc4I	CCNNNNNNNGG	5 cut(s)	135, 303, 1367, 1877, 1953
Bse1I	ACTGG	3 cut(s)	1644, 1804, 2290
Bse3DI	GCAATG	5 cut(s)	1164, 1566, 1928, 2058, 2163
Bse8I	GATNNNNATC	1 cut(s)	631
BseBI	CCWGG	3 cut(s)	451, 1282, 1468
BseDI	CCNNGG	2 cut(s)	1547, 1627
BseGI	GGATG	8 cut(s)	49, 333, 793, 979, 1426, 1897, 2114, 2213
BseJI	GATNNNNATC	1 cut(s)	631
BseLI	CCNNNNNNNGG	5 cut(s)	135, 303, 1367, 1877, 1953
BseMI	GCAATG	5 cut(s)	1164, 1566, 1928, 2058, 2163
BseMII	CTCAG	9 cut(s)	237, 417, 429, 638, 647, 663, 1104, 1985, 2088
BseNI	ACTGG	3 cut(s)	1644, 1804, 2290
BseRI	GAGGAG	3 cut(s)	65, 564, 1091
Bsh1236I	CGCG	1 cut(s)	1305
BshFI	GGCC	3 cut(s)	875, 1957, 2223
BshNI	GGYRCC	2 cut(s)	375, 516
BsiHKAI	GWGCWC	3 cut(s)	662, 1768, 2256
BsiSI	CCGG	3 cut(s)	129, 297, 1947
BslFI	GGGAC	1 cut(s)	2258
BslI	CCNNNNNNNGG	5 cut(s)	135, 303, 1367, 1877, 1953
BsmAI	GTCTC	4 cut(s)	47, 531, 815, 1175
BsmFI	GGGAC	1 cut(s)	2258
BsnI	GGCC	3 cut(s)	875, 1957, 2223
Bsp119I	TTCGAA	2 cut(s)	1392, 1558
Bsp1286I	GDGCHC	4 cut(s)	662, 1104, 1768, 2256
Bsp19I	CCATGG	2 cut(s)	1547, 1627
BspANI	GGCC	3 cut(s)	875, 1957, 2223
BspCNI	CTCAG	9 cut(s)	238, 418, 430, 639, 648, 664, 1103, 1986, 2087
BspFNI	CGCG	1 cut(s)	1305
BspLI	GGNNCC	5 cut(s)	377, 518, 1453, 1782, 2274
BspMAI	CTGCAG	3 cut(s)	412, 667, 2142
BspPI	GGATC	7 cut(s)	200, 1268, 1702, 1775, 1788, 2028, 2150
BspT104I	TTCGAA	2 cut(s)	1392, 1558
BspT107I	GGYRCC	2 cut(s)	375, 516
BsrBI	CCGCTC	1 cut(s)	302
BsrDI	GCAATG	5 cut(s)	1164, 1566, 1928, 2058, 2163
BsrI	ACTGG	3 cut(s)	1644, 1804, 2290
BssECI	CCNNGG	2 cut(s)	1547, 1627
BssSI	CACGAG	1 cut(s)	2045
BssT1I	CCWWGG	2 cut(s)	1547, 1627
Bst2BI	CACGAG	1 cut(s)	2045
Bst2UI	CCWGG	3 cut(s)	451, 1282, 1468
Bst4CI	ACNGT	4 cut(s)	177, 1848, 2307, 2319
Bst6I	CTCTTC	5 cut(s)	128, 174, 342, 813, 1548
BstBAI	YACGTR	1 cut(s)	1748
BstBI	TTCGAA	2 cut(s)	1392, 1558
BstC8I	GCNNGC	6 cut(s)	105, 146, 939, 1207, 1522, 1968
BstDSI	CCRYGG	2 cut(s)	1547, 1627
BstF5I	GGATG	8 cut(s)	49, 333, 793, 979, 1426, 1897, 2114, 2213
BstFNI	CGCG	1 cut(s)	1305
BstH2I	RGCGCY	1 cut(s)	2017
BstHHI	GCGC	2 cut(s)	1305, 2016
BstMAI	GTCTC	4 cut(s)	47, 531, 815, 1175
BstMWI	GCNNNNNNNGC	7 cut(s)	860, 1963, 1988, 2048, 2057, 2066, 2075
BstNI	CCWGG	3 cut(s)	451, 1282, 1468
BstNSI	RCATGY	3 cut(s)	155, 1339, 1343
BstSCI	CCNGG	4 cut(s)	128, 449, 1280, 1466
BstSFI	CTRYAG	5 cut(s)	408, 663, 1572, 2138, 2315
BstSNI	TACGTA	1 cut(s)	1748
BstUI	CGCG	1 cut(s)	1305
BstV2I	GAAGAC	1 cut(s)	1515
BstX2I	RGATCY	2 cut(s)	1780, 2155
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	2 cut(s)	1780, 2155
BsuRI	GGCC	3 cut(s)	875, 1957, 2223
BtgI	CCRYGG	2 cut(s)	1547, 1627
BtrI	CACGTC	2 cut(s)	82, 2232
BtsCI	GGATG	8 cut(s)	49, 333, 793, 979, 1426, 1897, 2114, 2213
BtsI	GCAGTG	1 cut(s)	2260
BtsIMutI	CAGTG	3 cut(s)	1991, 2260, 2312
Cac8I	GCNNGC	6 cut(s)	105, 146, 939, 1207, 1522, 1968
CaiI	CAGNNNCTG	1 cut(s)	671
CfoI	GCGC	2 cut(s)	1305, 2016
Cfr13I	GGNCC	1 cut(s)	2272
CseI	GACGC	2 cut(s)	109, 1038
Csp6I	GTAC	2 cut(s)	517, 1486
CviAII	CATG	9 cut(s)	152, 397, 1336, 1340, 1397, 1548, 1628, 1769, 2149
CviQI	GTAC	2 cut(s)	517, 1486
DraIII	CACNNNGTG	1 cut(s)	1058
EaeI	YGGCCR	2 cut(s)	1955, 2221
Eam1104I	CTCTTC	5 cut(s)	128, 174, 342, 813, 1548
EarI	CTCTTC	5 cut(s)	128, 174, 342, 813, 1548
EciI	GGCGGA	1 cut(s)	1527
Ecl136II	GAGCTC	3 cut(s)	660, 1766, 2254
Eco105I	TACGTA	1 cut(s)	1748
Eco130I	CCWWGG	2 cut(s)	1547, 1627
Eco24I	GRGCYC	4 cut(s)	662, 1104, 1768, 2256
Eco47I	GGWCC	1 cut(s)	2272
Eco47III	AGCGCT	1 cut(s)	2015
Eco53kI	GAGCTC	3 cut(s)	660, 1766, 2254
Eco57I	CTGAAG	2 cut(s)	607, 827
EcoICRI	GAGCTC	3 cut(s)	660, 1766, 2254
EcoRI	GAATTC	1 cut(s)	1223
EcoRII	CCWGG	3 cut(s)	449, 1280, 1466
EcoT14I	CCWWGG	2 cut(s)	1547, 1627
EcoT22I	ATGCAT	2 cut(s)	1341, 1906
EcoT38I	GRGCYC	4 cut(s)	662, 1104, 1768, 2256
ErhI	CCWWGG	2 cut(s)	1547, 1627
FaeI	CATG	9 cut(s)	155, 400, 1339, 1343, 1400, 1551, 1631, 1772, 2152
FalI	AAGNNNNNCTT	6 cut(s)	921, 953, 1311, 1343, 1500, 1532
FaqI	GGGAC	1 cut(s)	2258
FatI	CATG	9 cut(s)	151, 396, 1335, 1339, 1396, 1547, 1627, 1768, 2148
FauI	CCCGC	3 cut(s)	24, 295, 1090
FbaI	TGATCA	1 cut(s)	1213
FblI	GTMKAC	1 cut(s)	713
FokI	GGATG	8 cut(s)	56, 340, 800, 986, 1413, 1904, 2101, 2220
FriOI	GRGCYC	4 cut(s)	662, 1104, 1768, 2256
FspBI	CTAG	9 cut(s)	204, 272, 483, 828, 942, 1043, 1178, 1961, 2027
GlaI	GCGC	2 cut(s)	1304, 2015
GsuI	CTGGAG	2 cut(s)	1052, 2273
HaeII	RGCGCY	1 cut(s)	2017
HaeIII	GGCC	3 cut(s)	875, 1957, 2223
HapII	CCGG	3 cut(s)	129, 297, 1947
HgaI	GACGC	2 cut(s)	109, 1038
HhaI	GCGC	2 cut(s)	1305, 2016
Hin1II	CATG	9 cut(s)	155, 400, 1339, 1343, 1400, 1551, 1631, 1772, 2152
Hin6I	GCGC	2 cut(s)	1303, 2014
HinP1I	GCGC	2 cut(s)	1303, 2014
HincII	GTYRAC	1 cut(s)	714
HindII	GTYRAC	1 cut(s)	714
HindIII	AAGCTT	3 cut(s)	266, 1207, 2099
HinfI	GANTC	8 cut(s)	289, 551, 632, 641, 986, 1324, 1463, 1817
HpaII	CCGG	3 cut(s)	129, 297, 1947
HphI	GGTGA	5 cut(s)	127, 286, 764, 979, 1729
Hpy166II	GTNNAC	3 cut(s)	157, 714, 1828
Hpy188III	TCNNGA	9 cut(s)	272, 1105, 1411, 1460, 1784, 1793, 1837, 1878, 2249
Hpy8I	GTNNAC	3 cut(s)	157, 714, 1828
Hpy99I	CGWCG	2 cut(s)	103, 2236
HpyCH4III	ACNGT	4 cut(s)	177, 1848, 2307, 2319
HpyCH4IV	ACGT	7 cut(s)	81, 1562, 1635, 1747, 1938, 2081, 2231
HpyF10VI	GCNNNNNNNGC	7 cut(s)	860, 1963, 1988, 2048, 2057, 2066, 2075
HpySE526I	ACGT	7 cut(s)	81, 1562, 1635, 1747, 1938, 2081, 2231
Hsp92II	CATG	9 cut(s)	155, 400, 1339, 1343, 1400, 1551, 1631, 1772, 2152
HspAI	GCGC	2 cut(s)	1303, 2014
KpnI	GGTACC	1 cut(s)	520
Ksp22I	TGATCA	1 cut(s)	1213
LmnI	GCTCC	4 cut(s)	6, 221, 299, 1763
LweI	GCATC	6 cut(s)	658, 721, 778, 841, 1435, 1704
MaeI	CTAG	9 cut(s)	204, 272, 483, 828, 942, 1043, 1178, 1961, 2027
MaeII	ACGT	7 cut(s)	81, 1562, 1635, 1747, 1938, 2081, 2231
MaeIII	GTNAC	5 cut(s)	234, 1050, 1438, 1646, 1751
MbiI	CCGCTC	1 cut(s)	302
MfeI	CAATTG	1 cut(s)	1382
MflI	RGATCY	2 cut(s)	1780, 2155
MhlI	GDGCHC	4 cut(s)	662, 1104, 1768, 2256
MlsI	TGGCCA	1 cut(s)	1957
MluNI	TGGCCA	1 cut(s)	1957
MmeI	TCCRAC	2 cut(s)	21, 672
Mox20I	TGGCCA	1 cut(s)	1957
Mph1103I	ATGCAT	2 cut(s)	1341, 1906
MroXI	GAANNNNTTC	1 cut(s)	122
MscI	TGGCCA	1 cut(s)	1957
MseI	TTAA	6 cut(s)	228, 800, 947, 1290, 1350, 1859
MslI	CAYNNNNRTG	3 cut(s)	1152, 1338, 1996
Msp20I	TGGCCA	1 cut(s)	1957
MspA1I	CMGCKG	2 cut(s)	407, 1094
MspI	CCGG	3 cut(s)	129, 297, 1947
MspR9I	CCNGG	4 cut(s)	130, 451, 1282, 1468
MunI	CAATTG	1 cut(s)	1382
MvaI	CCWGG	3 cut(s)	451, 1282, 1468
MvnI	CGCG	1 cut(s)	1305
MwoI	GCNNNNNNNGC	7 cut(s)	860, 1963, 1988, 2048, 2057, 2066, 2075
NciI	CCSGG	1 cut(s)	130
NcoI	CCATGG	2 cut(s)	1547, 1627
NlaIII	CATG	9 cut(s)	155, 400, 1339, 1343, 1400, 1551, 1631, 1772, 2152
NlaIV	GGNNCC	5 cut(s)	377, 518, 1453, 1782, 2274
NmeAIII	GCCGAG	1 cut(s)	352
NmuCI	GTSAC	2 cut(s)	1050, 1751
NsiI	ATGCAT	2 cut(s)	1341, 1906
NspI	RCATGY	3 cut(s)	155, 1339, 1343
NspV	TTCGAA	2 cut(s)	1392, 1558
OliI	CACNNNNGTG	1 cut(s)	1996
PciI	ACATGT	1 cut(s)	151
PdmI	GAANNNNTTC	1 cut(s)	122
PfeI	GAWTC	8 cut(s)	289, 551, 632, 641, 986, 1324, 1463, 1817
PfoI	TCCNGGA	2 cut(s)	449, 1280
Ppu21I	YACGTR	1 cut(s)	1748
PscI	ACATGT	1 cut(s)	151
PsiI	TTATAA	2 cut(s)	1317, 1685
Psp124BI	GAGCTC	3 cut(s)	662, 1768, 2256
Psp6I	CCWGG	3 cut(s)	449, 1280, 1466
PspGI	CCWGG	3 cut(s)	449, 1280, 1466
PspN4I	GGNNCC	5 cut(s)	377, 518, 1453, 1782, 2274
PspPI	GGNCC	1 cut(s)	2272
PstI	CTGCAG	3 cut(s)	412, 667, 2142
PstNI	CAGNNNCTG	1 cut(s)	671
PsuI	RGATCY	2 cut(s)	1780, 2155
PvuII	CAGCTG	2 cut(s)	407, 1094
RsaI	GTAC	2 cut(s)	518, 1487
RsaNI	GTAC	2 cut(s)	517, 1486
RseI	CAYNNNNRTG	3 cut(s)	1152, 1338, 1996
SacI	GAGCTC	3 cut(s)	662, 1768, 2256
SalI	GTCGAC	1 cut(s)	712
SaqAI	TTAA	6 cut(s)	228, 800, 947, 1290, 1350, 1859
Sau96I	GGNCC	1 cut(s)	2272
ScrFI	CCNGG	4 cut(s)	130, 451, 1282, 1468
SduI	GDGCHC	4 cut(s)	662, 1104, 1768, 2256
SfaNI	GCATC	6 cut(s)	658, 721, 778, 841, 1435, 1704
SfcI	CTRYAG	5 cut(s)	408, 663, 1572, 2138, 2315
SfuI	TTCGAA	2 cut(s)	1392, 1558
SinI	GGWCC	1 cut(s)	2272
SmiMI	CAYNNNNRTG	3 cut(s)	1152, 1338, 1996
SmlI	CTYRAG	2 cut(s)	1411, 2249
SmoI	CTYRAG	2 cut(s)	1411, 2249
SnaBI	TACGTA	1 cut(s)	1748
SspMI	CTAG	9 cut(s)	204, 272, 483, 828, 942, 1043, 1178, 1961, 2027
SstI	GAGCTC	3 cut(s)	662, 1768, 2256
StyD4I	CCNGG	4 cut(s)	128, 449, 1280, 1466
StyI	CCWWGG	2 cut(s)	1547, 1627
TaaI	ACNGT	4 cut(s)	177, 1848, 2307, 2319
TaiI	ACGT	7 cut(s)	84, 1565, 1638, 1750, 1941, 2084, 2234
TaqI	TCGA	7 cut(s)	179, 345, 360, 713, 1392, 1558, 2256
TauI	GCSGC	2 cut(s)	1426, 2223
TfiI	GAWTC	8 cut(s)	289, 551, 632, 641, 986, 1324, 1463, 1817
Tru1I	TTAA	6 cut(s)	228, 800, 947, 1290, 1350, 1859
Tru9I	TTAA	6 cut(s)	228, 800, 947, 1290, 1350, 1859
TscAI	CASTG	3 cut(s)	1998, 2267, 2312
TseFI	GTSAC	2 cut(s)	1050, 1751
Tsp45I	GTSAC	2 cut(s)	1050, 1751
TspDTI	ATGAA	5 cut(s)	333, 429, 564, 1151, 2052
TspGWI	ACGGA	1 cut(s)	1266
TspRI	CASTG	3 cut(s)	1998, 2267, 2312
VpaK11BI	GGWCC	1 cut(s)	2272
XapI	RAATTY	4 cut(s)	812, 1223, 1554, 1687
XbaI	TCTAGA	1 cut(s)	271
XceI	RCATGY	3 cut(s)	155, 1339, 1343
XmiI	GTMKAC	1 cut(s)	713
XmnI	GAANNNNTTC	1 cut(s)	122
XspI	CTAG	9 cut(s)	204, 272, 483, 828, 942, 1043, 1178, 1961, 2027
Zsp2I	ATGCAT	2 cut(s)	1341, 1906

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.