Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0008602

Physical Location & Seq

Genomic Coordinates Reverse (-)

3439283 .. 3443285

Gene Structure 4003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00281040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGGGGAGCGTTGAAGAAGAGCAAGTGTGGGATGTGGAAGAGACCATCTTTGTTGCGGTGGGGGACGACGTGAAGCAGAGCGAAACGACGCTGTTTTGGGCCGTCAAGAACTTCGTCGGCAAGAATATATGCCTCCTTCACGTTCACCGCCCTTCATTGTGTGATGAAAATAGTTCAGTAAATTTACGAAATCTTCTAAACGAATACCTACTGATTCTGATCGGAGCAGGAGTGCAGGCAAATGAAGTACGGATTGAGAAGGACAATGTGGAGGAGGGGATTGTTGAATTGATTGCTCGGCACGATATTAGGTGGCTTGTCATGGGGGCAGCTGCAGATGAACATTATACCGAGAGATTGGCGGAAATAAGGGGAAACAGAGAAGATAATTCTGAAAGAGAAAGTGCCCCTCCTTTGGTGCTGCTGAATTCGGCTACTGGAACAGAGGAACCGGAGCACTTAAGTACAGAATCAATTCTTTGGTTGAGAAATCTAGATGCTGAGGAAGATGTCAATGAAGTGGAAGATCTGTCAAGGAGGTTGACCCCATTACTGATAGATGAGGAGGAAAAAGCAGCTGGTGGAAGTTATGATAACGTAACGCAAGCCATTTTGGACAGAAAGAACTCAAACCAAATGGAATTTGAAGATACAGCAAGGAGGTGGAAAGTGGAAGATGATGCCATGGAGGCCAAATGCAAAGCCAAAGCATTTGAAAGCTTATGCACTAAGGAGATGAGCCAAAGAAAAGAGTTGGAAGAATTGCTTGCTACAATTAGCCAGGAAACAGAGGGGTTGAAGGATGAACGTGATAAATATGTAGAACAAGTCCAGATAGTCCGGGACAAGAACGTAGTGCTGGAGGGCCAACTAGCAGAGTCACAATGTACAGTAAAGGAGTTAGAGGAGAAGATCATCTCTGCGGTGAAGCTCTTGATTAGTTTTAGAGAAAAACGGGATAACGTGCAAGCAGAGTATAGAAATGCAATCAGAAAAGTCAAACAGCTAAGCACATTAGGGGAAGCTGCAAGCTTTTGTCATGCAGAATTCCCCGTATTCTCTTTCATGGAGATCAATGAGGCAACTCAGAATTTTGACCCATCCCAGAAGATTGGAGAAGGAAGGTATGGAAGTGTTTATAAAGGGATTCTTCGCCACATGCATGTTGCAATAAAGATGTTGCCCTCTTATGGTTCTAAAAGGCAATTAGACTTCCAAAATGAGGTGGAGGTCTTGAGCAGAGCAAGGCATCCAAACCTGGTAACAGTAATTGGAATGTGTCCAGAATCTAGGTCACTTGTGTATGAATACCTTAAAAATGGCAGCCTTGAGGACCGCCTTGCAGGCAAAGACAATGCTCCACCTCTTCCATGGCAAATTCGAACATGCATTGCTACTGAAATATGCTCTGCCCTTATCTTCCTTCACTCAAATATCCCATGTATTGTCCATGGGAATCTAAAAGCAAGTAACATTCTCTTGGATGCAAACTTTGTGAGCAAACTTGGTGATTTGGGCATCGTTTCCTTAATTTCACAAAATGAAGATCCGGCCAACTTCACCAAAATATGTAATGACCCAAATGGAACTTTTGTGTATATGGATCCAGAGTATCTCGAGACAGGAAAGCTTACAAGAGAATCAGATGTTTACTCATTTGGGATTTTATTATTACGACTTTTAACCGCAAGGCCACTTTTGGGGATAGTGAAAGATGTGAAATGTGCACTAGAGAATGACAGCATCAGTGGTTTGTTGGACGTTTCAGCTGGAGACTGGCCGCTAGAGCAAGCAAAGGAGTTGGCTCTCTTAGCAATGAGGTGTTGTGAGGAGAACCGGTTGCATCGGATGGACCTATCTGTCGTCCATGAGGTAATGAGAACTTCGTGTGCTGCCTCTGAGTCGTGCTCGATTTCTAAGAAGCTTCTCCGAACACCATCCCATTTCGTCTGCCCCATTCTTCAGGAAGTCATGTCGGATCCACGTATAGCAGCAGATGGTTACACATATGAAGAAGAGGCAATACAAGGATGGTTCAAGAGCGGACACAGTACTTCTCCGATGACAAATCTTGAGCTCGAACACTGCAATCTGGTTCCCAACTATGCGCTTCAGTTTGCAATTCAACAGTGGCAACTACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

714

Amino Acids

80.56

Weight (kDa)

4.9

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	368 - 624	7.7e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	368 - 618	5e-37	Protein kinase domain
U-box	PF04564	646 - 712	1.1e-15	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49060 AT3G49060 AT3G49060
fragaria_vesca	FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840 FvH4_7g29840
malus_domestica	MD01G1205000.v1.1
prunus_persica	Prupe.2G297400_v2.0.a1
pyrus_communis	pycom01g21460
rosa_chinensis	RchiOBHm_Chr1g0377811
rosa_laevigata	RLG00000026496
rosa_multiflora	Rmu_co8492821.1_g000001 Rmu_sc0004824.1_g000003
rosa_roxburghii	Rroxscaffold_4G00281040
rosa_rugosa	Rorug01G0405200
rosa_samantha	Rh1AG427100 Rh1BG382200 Rh1CG396100 Rh1DG413200
rosa_wichuraiana	Rw1G037150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1140
AasI	GACNNNNNNGTC	1 cut(s)	1859
AccBSI	CCGCTC	1 cut(s)	2043
AciI	CCGC	8 cut(s)	56, 148, 362, 923, 1336, 1686, 1781, 2043
AclWI	GGATC	5 cut(s)	1541, 1598, 1611, 1973, 1986
AcoI	YGGCCR	2 cut(s)	1551, 1778
AcsI	RAATTY	6 cut(s)	181, 427, 641, 1046, 1090, 1377
AcuI	CTGAAG	2 cut(s)	1946, 2096
AfaI	GTAC	4 cut(s)	249, 466, 889, 2053
AfiI	CCNNNNNNNGG	4 cut(s)	415, 1190, 1222, 1700
AflII	CTTAAG	1 cut(s)	460
AgeI	ACCGGT	1 cut(s)	1836
AgsI	TTSAA	7 cut(s)	14, 287, 647, 716, 799, 2038, 2126
AjiI	CACGTC	1 cut(s)	70
AjnI	CCWGG	2 cut(s)	780, 1257
Alw21I	GWGCWC	4 cut(s)	459, 1729, 1910, 2079
Alw26I	GTCTC	3 cut(s)	35, 1613, 1767
Alw44I	GTGCAC	1 cut(s)	1725
AlwI	GGATC	5 cut(s)	1541, 1598, 1611, 1973, 1986
Ama87I	CYCGRG	1 cut(s)	1616
AoxI	GGCC	6 cut(s)	99, 690, 865, 1551, 1691, 1778
ApaLI	GTGCAC	1 cut(s)	1725
ApeKI	GCWGC	8 cut(s)	329, 332, 421, 575, 1025, 1323, 1892, 1991
ApoI	RAATTY	6 cut(s)	181, 427, 641, 1046, 1090, 1377
AsiGI	ACCGGT	1 cut(s)	1836
Asp700I	GAANNNNTTC	1 cut(s)	474
AspLEI	GCGC	1 cut(s)	2110
AspS9I	GGNCC	4 cut(s)	99, 865, 1333, 1852
AsuC2I	CCSGG	1 cut(s)	842
AsuHPI	GGTGA	4 cut(s)	137, 937, 1520, 1552
AsuII	TTCGAA	1 cut(s)	1381
AvaI	CYCGRG	1 cut(s)	1616
AvaII	GGWCC	2 cut(s)	1333, 1852
BaeGI	GKGCMC	2 cut(s)	409, 1729
BamHI	GGATCC	2 cut(s)	1603, 1978
BanII	GRGCYC	1 cut(s)	2079
BarI	GAAGNNNNNNTAC	2 cut(s)	2007, 2039
Bbv12I	GWGCWC	4 cut(s)	459, 1729, 1910, 2079
BbvCI	CCTCAGC	1 cut(s)	501
BbvI	GCAGC	8 cut(s)	319, 341, 408, 587, 1012, 1335, 1879, 2003
BccI	CCATC	6 cut(s)	53, 1108, 1843, 1945, 1991, 2025
BceAI	ACGGC	1 cut(s)	86
BciT130I	CCWGG	2 cut(s)	782, 1259
BcnI	CCSGG	1 cut(s)	842
BcoDI	GTCTC	3 cut(s)	35, 1613, 1767
BfaI	CTAG	5 cut(s)	494, 872, 1290, 1730, 1784
BfmI	CTRYAG	2 cut(s)	333, 2138
BfrI	CTTAAG	1 cut(s)	460
BglI	GCCNNNNNGGC	2 cut(s)	689, 1344
BglII	AGATCT	1 cut(s)	526
BisI	GCNGC	9 cut(s)	330, 333, 422, 576, 1026, 1324, 1781, 1893, 1992
BlpI	GCTNAGC	1 cut(s)	1007
BlsI	GCNGC	9 cut(s)	331, 334, 423, 577, 1027, 1325, 1782, 1894, 1993
BmcAI	AGTACT	1 cut(s)	2053
Bme1390I	CCNGG	3 cut(s)	782, 842, 1259
Bme18I	GGWCC	2 cut(s)	1333, 1852
BmeT110I	CYCGRG	1 cut(s)	1616
BmgBI	CACGTC	1 cut(s)	70
BmgT120I	GGNCC	4 cut(s)	99, 865, 1333, 1852
BmiI	GGNNCC	4 cut(s)	450, 1605, 1980, 2097
BmrFI	CCNGG	3 cut(s)	782, 842, 1259
BmsI	GCATC	7 cut(s)	487, 670, 1258, 1474, 1527, 1752, 1852
BplI	GAGNNNNNCTC	2 cut(s)	1892, 1924
BpmI	CTGGAG	2 cut(s)	881, 1791
Bpu10I	CCTNAGC	1 cut(s)	501
Bpu1102I	GCTNAGC	1 cut(s)	1007
Bpu14I	TTCGAA	1 cut(s)	1381
BpuEI	CTTGAG	3 cut(s)	1255, 1349, 2093
BpuMI	CCSGG	1 cut(s)	842
BsaAI	YACGTR	1 cut(s)	1985
BsaBI	GATNNNNATC	1 cut(s)	218
BsaI	GGTCTC	1 cut(s)	35
BsaJI	CCNNGG	3 cut(s)	684, 1370, 1450
BsaWI	WCCGGW	2 cut(s)	451, 1836
BsaXI	ACNNNNNCTCC	4 cut(s)	216, 246, 1823, 1853
Bsc4I	CCNNNNNNNGG	4 cut(s)	415, 1190, 1222, 1700
Bse118I	RCCGGY	1 cut(s)	1836
Bse1I	ACTGG	2 cut(s)	442, 1781
Bse3DI	GCAATG	2 cut(s)	1389, 1821
Bse8I	GATNNNNATC	1 cut(s)	218
BseBI	CCWGG	2 cut(s)	782, 1259
BseDI	CCNNGG	3 cut(s)	684, 1370, 1450
BseGI	GGATG	8 cut(s)	37, 808, 1100, 1249, 1489, 1854, 1937, 2036
BseJI	GATNNNNATC	1 cut(s)	218
BseLI	CCNNNNNNNGG	4 cut(s)	415, 1190, 1222, 1700
BseMI	GCAATG	2 cut(s)	1389, 1821
BseMII	CTCAG	3 cut(s)	492, 1100, 1890
BseNI	ACTGG	2 cut(s)	442, 1781
BseRI	GAGGAG	4 cut(s)	287, 578, 920, 1844
BseSI	GKGCMC	2 cut(s)	409, 1729
BseXI	GCAGC	8 cut(s)	319, 341, 408, 587, 1012, 1335, 1879, 2003
BsgI	GTGCAG	1 cut(s)	254
BshFI	GGCC	6 cut(s)	101, 692, 867, 1553, 1693, 1780
BshTI	ACCGGT	1 cut(s)	1836
BsiHKAI	GWGCWC	4 cut(s)	459, 1729, 1910, 2079
BsiHKCI	CYCGRG	1 cut(s)	1616
BsiSI	CCGG	4 cut(s)	452, 841, 1550, 1837
BslFI	GGGAC	2 cut(s)	77, 857
BslI	CCNNNNNNNGG	4 cut(s)	415, 1190, 1222, 1700
BsmAI	GTCTC	3 cut(s)	35, 1613, 1767
BsmFI	GGGAC	2 cut(s)	77, 857
BsnI	GGCC	6 cut(s)	101, 692, 867, 1553, 1693, 1780
Bso31I	GGTCTC	1 cut(s)	35
BsoBI	CYCGRG	1 cut(s)	1616
Bsp119I	TTCGAA	1 cut(s)	1381
Bsp1286I	GDGCHC	5 cut(s)	409, 459, 1729, 1910, 2079
Bsp1407I	TGTACA	1 cut(s)	887
Bsp143I	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
Bsp1720I	GCTNAGC	1 cut(s)	1007
Bsp19I	CCATGG	3 cut(s)	684, 1370, 1450
BspACI	CCGC	8 cut(s)	56, 148, 362, 923, 1336, 1686, 1781, 2043
BspANI	GGCC	6 cut(s)	101, 692, 867, 1553, 1693, 1780
BspCNI	CTCAG	3 cut(s)	493, 1099, 1891
BspLI	GGNNCC	4 cut(s)	450, 1605, 1980, 2097
BspMAI	CTGCAG	1 cut(s)	337
BspPI	GGATC	5 cut(s)	1541, 1598, 1611, 1973, 1986
BspQI	GCTCTTC	1 cut(s)	12
BspT104I	TTCGAA	1 cut(s)	1381
BspTI	CTTAAG	1 cut(s)	460
BspTNI	GGTCTC	1 cut(s)	35
BsrBI	CCGCTC	1 cut(s)	2043
BsrDI	GCAATG	2 cut(s)	1389, 1821
BsrFI	RCCGGY	1 cut(s)	1836
BsrGI	TGTACA	1 cut(s)	887
BsrI	ACTGG	2 cut(s)	442, 1781
BssAI	RCCGGY	1 cut(s)	1836
BssECI	CCNNGG	3 cut(s)	684, 1370, 1450
BssMI	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
BssT1I	CCWWGG	3 cut(s)	684, 1370, 1450
Bst2UI	CCWGG	2 cut(s)	782, 1259
Bst4CI	ACNGT	5 cut(s)	892, 1267, 2051, 2130, 2142
Bst6I	CTCTTC	4 cut(s)	12, 33, 1371, 2010
BstAFI	CTTAAG	1 cut(s)	460
BstAUI	TGTACA	1 cut(s)	887
BstBAI	YACGTR	1 cut(s)	1985
BstBI	TTCGAA	1 cut(s)	1381
BstC8I	GCNNGC	7 cut(s)	237, 606, 768, 969, 1030, 1345, 1791
BstDEI	CTNAG	7 cut(s)	501, 729, 1007, 1086, 1810, 1899, 1917
BstDSI	CCRYGG	3 cut(s)	684, 1370, 1450
BstF5I	GGATG	8 cut(s)	37, 808, 1100, 1249, 1489, 1854, 1937, 2036
BstHHI	GCGC	1 cut(s)	2110
BstKTI	GATC	7 cut(s)	222, 529, 915, 1074, 1549, 1606, 1981
BstMAI	GTCTC	3 cut(s)	35, 1613, 1767
BstMBI	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
BstMWI	GCNNNNNNNGC	4 cut(s)	689, 1344, 1786, 1811
BstNI	CCWGG	2 cut(s)	782, 1259
BstNSI	RCATGY	3 cut(s)	1162, 1166, 1389
BstSCI	CCNGG	3 cut(s)	780, 840, 1257
BstSFI	CTRYAG	2 cut(s)	333, 2138
BstSLI	GKGCMC	2 cut(s)	409, 1729
BstV1I	GCAGC	8 cut(s)	319, 341, 408, 587, 1012, 1335, 1879, 2003
BstX2I	RGATCY	4 cut(s)	526, 1546, 1603, 1978
BstXI	CCANNNNNNTGG	1 cut(s)	1112
BstYI	RGATCY	4 cut(s)	526, 1546, 1603, 1978
BsuRI	GGCC	6 cut(s)	101, 692, 867, 1553, 1693, 1780
BtgI	CCRYGG	3 cut(s)	684, 1370, 1450
BtrI	CACGTC	1 cut(s)	70
BtsCI	GGATG	8 cut(s)	37, 808, 1100, 1249, 1489, 1854, 1937, 2036
BtsI	GCAGTG	1 cut(s)	2083
BtsIMutI	CAGTG	3 cut(s)	1753, 2083, 2135
Cac8I	GCNNGC	7 cut(s)	237, 606, 768, 969, 1030, 1345, 1791
CfoI	GCGC	1 cut(s)	2110
Cfr10I	RCCGGY	1 cut(s)	1836
Cfr13I	GGNCC	4 cut(s)	99, 865, 1333, 1852
CseI	GACGC	1 cut(s)	97
CsiI	ACCWGGT	1 cut(s)	1257
Csp6I	GTAC	4 cut(s)	248, 465, 888, 2052
CspAI	ACCGGT	1 cut(s)	1836
CviQI	GTAC	4 cut(s)	248, 465, 888, 2052
DdeI	CTNAG	7 cut(s)	501, 729, 1007, 1086, 1810, 1899, 1917
DpnI	GATC	7 cut(s)	221, 528, 914, 1073, 1548, 1605, 1980
DpnII	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
DrdI	GACNNNNNNGTC	1 cut(s)	1859
DseDI	GACNNNNNNGTC	1 cut(s)	1859
EaeI	YGGCCR	2 cut(s)	1551, 1778
Eam1104I	CTCTTC	4 cut(s)	12, 33, 1371, 2010
EarI	CTCTTC	4 cut(s)	12, 33, 1371, 2010
EciI	GGCGGA	1 cut(s)	377
Ecl136II	GAGCTC	1 cut(s)	2077
Eco130I	CCWWGG	3 cut(s)	684, 1370, 1450
Eco24I	GRGCYC	1 cut(s)	2079
Eco31I	GGTCTC	1 cut(s)	35
Eco47I	GGWCC	2 cut(s)	1333, 1852
Eco53kI	GAGCTC	1 cut(s)	2077
Eco57I	CTGAAG	2 cut(s)	1946, 2096
Eco88I	CYCGRG	1 cut(s)	1616
EcoICRI	GAGCTC	1 cut(s)	2077
EcoRI	GAATTC	2 cut(s)	427, 1046
EcoRII	CCWGG	2 cut(s)	780, 1257
EcoT14I	CCWWGG	3 cut(s)	684, 1370, 1450
EcoT22I	ATGCAT	2 cut(s)	1164, 1391
EcoT38I	GRGCYC	1 cut(s)	2079
ErhI	CCWWGG	3 cut(s)	684, 1370, 1450
FalI	AAGNNNNNCTT	4 cut(s)	750, 782, 1134, 1166
FaqI	GGGAC	2 cut(s)	77, 857
FauNDI	CATATG	1 cut(s)	2008
Fnu4HI	GCNGC	9 cut(s)	330, 333, 422, 576, 1026, 1324, 1781, 1893, 1992
FokI	GGATG	8 cut(s)	44, 815, 1087, 1236, 1496, 1861, 1924, 2043
FriOI	GRGCYC	1 cut(s)	2079
Fsp4HI	GCNGC	9 cut(s)	330, 333, 422, 576, 1026, 1324, 1781, 1893, 1992
FspBI	CTAG	5 cut(s)	494, 872, 1290, 1730, 1784
GlaI	GCGC	1 cut(s)	2109
GluI	GCNGC	9 cut(s)	330, 333, 422, 576, 1026, 1324, 1781, 1893, 1992
GsuI	CTGGAG	2 cut(s)	881, 1791
HaeIII	GGCC	6 cut(s)	101, 692, 867, 1553, 1693, 1780
HapII	CCGG	4 cut(s)	452, 841, 1550, 1837
HgaI	GACGC	1 cut(s)	97
HhaI	GCGC	1 cut(s)	2110
Hin6I	GCGC	1 cut(s)	2108
HinP1I	GCGC	1 cut(s)	2108
HincII	GTYRAC	1 cut(s)	543
HindII	GTYRAC	1 cut(s)	543
HindIII	AAGCTT	4 cut(s)	718, 1030, 1628, 1922
HinfI	GANTC	8 cut(s)	214, 470, 878, 1147, 1286, 1456, 1640, 1901
HpaII	CCGG	4 cut(s)	452, 841, 1550, 1837
HphI	GGTGA	4 cut(s)	137, 937, 1520, 1552
Hpy166II	GTNNAC	4 cut(s)	145, 543, 1651, 1727
Hpy8I	GTNNAC	4 cut(s)	145, 543, 1651, 1727
Hpy99I	CGWCG	3 cut(s)	71, 91, 119
HpyAV	CCTTC	7 cut(s)	146, 162, 253, 793, 1112, 1116, 1433
HpyCH4III	ACNGT	5 cut(s)	892, 1267, 2051, 2130, 2142
HpyCH4IV	ACGT	8 cut(s)	69, 141, 597, 808, 852, 963, 1761, 1984
HpyF10VI	GCNNNNNNNGC	4 cut(s)	689, 1344, 1786, 1811
HpyF3I	CTNAG	7 cut(s)	501, 729, 1007, 1086, 1810, 1899, 1917
HpySE526I	ACGT	8 cut(s)	69, 141, 597, 808, 852, 963, 1761, 1984
HspAI	GCGC	1 cut(s)	2108
Kzo9I	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	4 cut(s)	6, 224, 454, 1363
Lsp1109I	GCAGC	8 cut(s)	319, 341, 408, 587, 1012, 1335, 1879, 2003
LweI	GCATC	7 cut(s)	487, 670, 1258, 1474, 1527, 1752, 1852
MabI	ACCWGGT	1 cut(s)	1257
MaeI	CTAG	5 cut(s)	494, 872, 1290, 1730, 1784
MaeII	ACGT	8 cut(s)	69, 141, 597, 808, 852, 963, 1761, 1984
MaeIII	GTNAC	6 cut(s)	598, 879, 1261, 1293, 1469, 2000
MalI	GATC	7 cut(s)	221, 528, 914, 1073, 1548, 1605, 1980
MbiI	CCGCTC	1 cut(s)	2043
MboI	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
MflI	RGATCY	4 cut(s)	526, 1546, 1603, 1978
MhlI	GDGCHC	5 cut(s)	409, 459, 1729, 1910, 2079
MlyI	GAGTC	2 cut(s)	887, 1910
MmeI	TCCRAC	3 cut(s)	735, 1737, 1956
Mph1103I	ATGCAT	2 cut(s)	1164, 1391
MroXI	GAANNNNTTC	1 cut(s)	474
MseI	TTAA	4 cut(s)	461, 1314, 1529, 1682
MslI	CAYNNNNRTG	1 cut(s)	1161
MspA1I	CMGCKG	3 cut(s)	332, 578, 1769
MspCI	CTTAAG	1 cut(s)	460
MspI	CCGG	4 cut(s)	452, 841, 1550, 1837
MspR9I	CCNGG	3 cut(s)	782, 842, 1259
MvaI	CCWGG	2 cut(s)	782, 1259
MwoI	GCNNNNNNNGC	4 cut(s)	689, 1344, 1786, 1811
NciI	CCSGG	1 cut(s)	842
NcoI	CCATGG	3 cut(s)	684, 1370, 1450
NdeI	CATATG	1 cut(s)	2008
NdeII	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
NlaIV	GGNNCC	4 cut(s)	450, 1605, 1980, 2097
NmeAIII	GCCGAG	1 cut(s)	277
NmuCI	GTSAC	2 cut(s)	879, 1293
NsiI	ATGCAT	2 cut(s)	1164, 1391
NspI	RCATGY	3 cut(s)	1162, 1166, 1389
NspV	TTCGAA	1 cut(s)	1381
PaeR7I	CTCGAG	1 cut(s)	1616
PciSI	GCTCTTC	1 cut(s)	12
PdmI	GAANNNNTTC	1 cut(s)	474
PfeI	GAWTC	6 cut(s)	214, 470, 1147, 1286, 1456, 1640
PfoI	TCCNGGA	1 cut(s)	840
PinAI	ACCGGT	1 cut(s)	1836
PkrI	GCNGC	9 cut(s)	331, 334, 423, 577, 1027, 1325, 1782, 1894, 1993
PleI	GAGTC	2 cut(s)	886, 1909
PpsI	GAGTC	2 cut(s)	886, 1909
Ppu21I	YACGTR	1 cut(s)	1985
PsiI	TTATAA	1 cut(s)	1140
Psp124BI	GAGCTC	1 cut(s)	2079
Psp6I	CCWGG	2 cut(s)	780, 1257
PspGI	CCWGG	2 cut(s)	780, 1257
PspN4I	GGNNCC	4 cut(s)	450, 1605, 1980, 2097
PspPI	GGNCC	4 cut(s)	99, 865, 1333, 1852
PstI	CTGCAG	1 cut(s)	337
PsuI	RGATCY	4 cut(s)	526, 1546, 1603, 1978
PvuII	CAGCTG	3 cut(s)	332, 578, 1769
RsaI	GTAC	4 cut(s)	249, 466, 889, 2053
RsaNI	GTAC	4 cut(s)	248, 465, 888, 2052
RseI	CAYNNNNRTG	1 cut(s)	1161
SacI	GAGCTC	1 cut(s)	2079
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	4 cut(s)	461, 1314, 1529, 1682
SatI	GCNGC	9 cut(s)	330, 333, 422, 576, 1026, 1324, 1781, 1893, 1992
Sau3AI	GATC	7 cut(s)	219, 526, 912, 1071, 1546, 1603, 1978
Sau96I	GGNCC	4 cut(s)	99, 865, 1333, 1852
ScaI	AGTACT	1 cut(s)	2053
SchI	GAGTC	2 cut(s)	887, 1910
ScrFI	CCNGG	3 cut(s)	782, 842, 1259
SduI	GDGCHC	5 cut(s)	409, 459, 1729, 1910, 2079
SexAI	ACCWGGT	1 cut(s)	1257
SfaNI	GCATC	7 cut(s)	487, 670, 1258, 1474, 1527, 1752, 1852
SfcI	CTRYAG	2 cut(s)	333, 2138
Sfr274I	CTCGAG	1 cut(s)	1616
SfuI	TTCGAA	1 cut(s)	1381
SinI	GGWCC	2 cut(s)	1333, 1852
SlaI	CTCGAG	1 cut(s)	1616
SmiMI	CAYNNNNRTG	1 cut(s)	1161
SmlI	CTYRAG	5 cut(s)	460, 1234, 1328, 1616, 2072
SmoI	CTYRAG	5 cut(s)	460, 1234, 1328, 1616, 2072
SsiI	CCGC	8 cut(s)	56, 148, 362, 923, 1336, 1686, 1781, 2043
SspMI	CTAG	5 cut(s)	494, 872, 1290, 1730, 1784
SstI	GAGCTC	1 cut(s)	2079
StyD4I	CCNGG	3 cut(s)	780, 840, 1257
StyI	CCWWGG	3 cut(s)	684, 1370, 1450
TaaI	ACNGT	5 cut(s)	892, 1267, 2051, 2130, 2142
TaiI	ACGT	8 cut(s)	72, 144, 600, 811, 855, 966, 1764, 1987
TaqI	TCGA	4 cut(s)	1381, 1617, 1910, 2079
TatI	WGTACW	3 cut(s)	464, 887, 2051
TauI	GCSGC	1 cut(s)	1783
TfiI	GAWTC	6 cut(s)	214, 470, 1147, 1286, 1456, 1640
Tru1I	TTAA	4 cut(s)	461, 1314, 1529, 1682
Tru9I	TTAA	4 cut(s)	461, 1314, 1529, 1682
TscAI	CASTG	3 cut(s)	1753, 2090, 2135
TseFI	GTSAC	2 cut(s)	879, 1293
TseI	GCWGC	8 cut(s)	329, 332, 421, 575, 1025, 1323, 1892, 1991
Tsp45I	GTSAC	2 cut(s)	879, 1293
TspGWI	ACGGA	1 cut(s)	265
TspRI	CASTG	3 cut(s)	1753, 2090, 2135
Vha464I	CTTAAG	1 cut(s)	460
VneI	GTGCAC	1 cut(s)	1725
VpaK11BI	GGWCC	2 cut(s)	1333, 1852
XapI	RAATTY	6 cut(s)	181, 427, 641, 1046, 1090, 1377
XbaI	TCTAGA	1 cut(s)	493
XceI	RCATGY	3 cut(s)	1162, 1166, 1389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1368
XhoI	CTCGAG	1 cut(s)	1616
XmnI	GAANNNNTTC	1 cut(s)	474
XspI	CTAG	5 cut(s)	494, 872, 1290, 1730, 1784
ZrmI	AGTACT	1 cut(s)	2053
Zsp2I	ATGCAT	2 cut(s)	1164, 1391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.