MD01G1212800.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015479
Physical Location & Seq
Forward (+)
30626146 .. 30630319
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1212800.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGGTGCAGGTGGTGGCTATGCGGGCGATTCAGAGCTCGATCCTCTCTCCCACCTCTGAATCCGCATACCGCCGCCCCGCCTTCAAGCTGAAGCCGCCAACGAGCTTTGCGTACAAAGTTATGGCCTTTGAGGAGAAGCAGAGGGGATGGAGCTGGAGACTGTTTGGGAACAGGAGGTCTGAGATCACTGCGAAGCGTCCCCTTCAGACGGAGGTCGCTCCCGTCTCACCCGAAGACTCACCCAAGATAGAGGAGCAGTGGAAGGCAATTCAGCAACTTGATGACCCAGCAGTGGGTATGTGGTCCAAGCCTGTTGTAAAACGTAAGACAAAGATTGTTTGCACAATTGGTCCTTCCACCAACACACGCGAAATGATCTGGAAGTTGGCCGAGGCTGGAATGAATGTTGCTCGTATGAATATGTCTCATGGAGACCACGCGTCTCATCAGAAAGTTATAGACTTGGTTAAAGAATATAATGAGCAACATAAGGACAATGTCATCGCAATCATGCTTGATACCAAGGCAAGGCTCCCCTTATTGTTTGGTCCTGAGGTTAGGAGCGGTGACTTGCCGCAGCCAATCAATTTGGAAAGCGGGCAGGAATTTACCTTTACAATCAAAAGAGGGGTTGGCACATCAGATTGTGTTAGCGTGAACTATGATGATTTTGTTAACGATGTAGCACCGGGAGACATGCTTCTGGTTGATGGTGGTATGATGTCATTCCTGGTGAAGTCCAAGACAGAAGAATCAGTGAATTGTGAAGTTGTTGATGGAGGAGAGCTTAAGTCTAGGCGACATTTGAATGTGCGAGGGAAAAGTGCAACATTGCCTTCTATCACTGAGAAAGATTGGGAAGATATTAAATTTGGAGTGGACAATAAAGTTGACTTTTACGCTGTTTCATTTGTCAAAGATGCGCAAGTTGTTCATGAATTGAAGAATTATCTGCAAAGTAACAATGCAGATATACATGTTATCGTAAAAATAGAAAGTGCAGACTCTATCCCGAATTTGCATTCCATTATCACAGCATCCGATGGGGCAATGGTTGCAAGAGGAGATCTTGGTGCGGAGCTCCCTGTTGAAGAAGTTCCGCTTTTGCAGGAAGAGATTATCAGGATATGCCGTAGCATGGGAAAGGCTGTTATTGTGGCAACAAATATGCTGGAAAGCATGATTGTTCACCCAACACCAACCAGAGCTGAGGTATCCGACATTGCCATTGCTGTTAGAGAGGGTTCTGATGGAATTATGCTTTCTGGAGAAACTGCTCATGGAAAGTTTCCTTTGAAGGCAGTGAAAGTTATGCACACTGTCGCACTTCGGACTGAGGCGACCATCTTGGGTAGCGCAGTGCCAGCCAATCTTGGTAAAGCTTTCAAGAACCACATGAGTGAGATGTTTGCTTACCATGCAACCATGATGTCCAATACTCTTGGTACCTCAATCGTTGTCTTTACCAGAACTGGTTTCATGGCTATTCTATTGAGCCATTACCGACCTACCGGCACCATATTTGCCTTCACGAATGATAAGAGCATACAACGGAGATTGGCTTTGTATCAAGGCGTATGTCCCATATACATGGAGTTCACAGAAGATTCTGAAACAAGCTTTAGCAATGCCTTGACTGTGCTGAAGGAGCAAGGACTGGTAAAGGCAGGAGAAGAGGTCGCACTCGTTCAAAGTGGCAGGCAACCCATTTGGCGGCTTCAGTCCACTCACAACATTCAAGTGCGCAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

585

Amino Acids

64.72

Weight (kDa)

6.96

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 108 - 435 6.3e-108 Pyruvate kinase, barrel domain
PK_C PF02887 471 - 564 6.9e-20 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52920
fragaria_vesca FvH4_7g30750
malus_domestica MD01G1212800.v1.1 MD07G1282700.v1.1
prunus_persica Prupe.2G305000_v2.0.a1
pyrus_communis pycom01g22390 pycom07g25700
rosa_chinensis RchiOBHm_Chr1g0379701
rosa_laevigata RLG00000026366
rosa_multiflora Rmu_sc0013028.1_g000007
rosa_rugosa Rorug01G0414700
rosa_samantha Rh1AG438100 Rh1BG394600 Rh1CG407600 Rh1DG425000
rosa_wichuraiana Rw1G038180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 924, 1745
Acc65I GGTACC 1 cut(s) 1445
AccB1I GGYRCC 2 cut(s) 1445, 1514
AccBSI CCGCTC 1 cut(s) 564
AccII CGCG 2 cut(s) 369, 440
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 3 cut(s) 605, 869, 1015
AcuI CTGAAG 4 cut(s) 110, 188, 1664, 1703
AfaI GTAC 2 cut(s) 113, 1447
AfiI CCNNNNNNNGG 6 cut(s) 208, 292, 293, 528, 1138, 1713
AflII CTTAAG 1 cut(s) 788
AflIII ACRYGT 2 cut(s) 438, 976
AgsI TTSAA 8 cut(s) 85, 808, 943, 1091, 1297, 1387, 1691, 1739
AhdI GACNNNNNGTC 1 cut(s) 439
AjnI CCWGG 1 cut(s) 729
AleI CACNNNNGTG 1 cut(s) 1398
AluBI AGCT 9 cut(s) 36, 88, 105, 153, 787, 1081, 1208, 1382, 1620
AluI AGCT 9 cut(s) 36, 88, 105, 153, 787, 1081, 1208, 1382, 1620
Alw21I GWGCWC 2 cut(s) 38, 1083
Alw26I GTCTC 6 cut(s) 151, 229, 426, 429, 447, 687
AlwI GGATC 1 cut(s) 34
AoxI GGCC 2 cut(s) 123, 387
ApeKI GCWGC 1 cut(s) 577
ApoI RAATTY 3 cut(s) 605, 869, 1015
Asp700I GAANNNNTTC 1 cut(s) 1095
Asp718I GGTACC 1 cut(s) 1445
AspLEI GCGC 3 cut(s) 925, 1358, 1746
AspS9I GGNCC 3 cut(s) 303, 350, 548
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 5 cut(s) 219, 231, 578, 745, 1181
AvaII GGWCC 3 cut(s) 303, 350, 548
AxyI CCTNAGG 1 cut(s) 552
BanI GGYRCC 2 cut(s) 1445, 1514
BanII GRGCYC 2 cut(s) 38, 1083
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 38, 1083
BbvCI CCTCAGC 1 cut(s) 1209
BbvI GCAGC 1 cut(s) 589
BccI CCATC 6 cut(s) 141, 704, 770, 1037, 1244, 1352
BceAI ACGGC 1 cut(s) 1116
BciT130I CCWGG 1 cut(s) 731
BciVI GTATCC 1 cut(s) 1225
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 6 cut(s) 151, 229, 426, 429, 447, 687
BfaI CTAG 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 788
BfuI GTATCC 1 cut(s) 1225
BglII AGATCT 1 cut(s) 1066
BisI GCNGC 5 cut(s) 73, 95, 575, 578, 1715
BlsI GCNGC 5 cut(s) 74, 96, 576, 579, 1716
Bme1390I CCNGG 2 cut(s) 690, 731
Bme18I GGWCC 3 cut(s) 303, 350, 548
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 303, 350, 548
BmiI GGNNCC 3 cut(s) 533, 1447, 1516
BmrFI CCNGG 2 cut(s) 690, 731
BmsI GCATC 2 cut(s) 910, 1046
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 2 cut(s) 175, 1287
Bpu10I CCTNAGC 1 cut(s) 1209
BpuMI CCSGG 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 390, 522
BsaXI ACNNNNNCTCC 2 cut(s) 1662, 1692
Bsc4I CCNNNNNNNGG 6 cut(s) 208, 292, 293, 528, 1138, 1713
Bse118I RCCGGY 1 cut(s) 1511
Bse1I ACTGG 2 cut(s) 1477, 1662
Bse21I CCTNAGG 1 cut(s) 552
Bse3DI GCAATG 5 cut(s) 830, 1056, 1221, 1227, 1633
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 2 cut(s) 390, 522
BseGI GGATG 2 cut(s) 152, 1037
BseLI CCNNNNNNNGG 6 cut(s) 208, 292, 293, 528, 1138, 1713
BseMI GCAATG 5 cut(s) 830, 1056, 1221, 1227, 1633
BseMII CTCAG 5 cut(s) 171, 543, 837, 1200, 1326
BseNI ACTGG 2 cut(s) 1477, 1662
BseRI GAGGAG 4 cut(s) 146, 266, 795, 1077
BseXI GCAGC 1 cut(s) 589
BseYI CCCAGC 1 cut(s) 286
BsgI GTGCAG 2 cut(s) 26, 1020
Bsh1236I CGCG 2 cut(s) 369, 440
BshFI GGCC 2 cut(s) 125, 389
BshNI GGYRCC 2 cut(s) 1445, 1514
BsiHKAI GWGCWC 2 cut(s) 38, 1083
BsiSI CCGG 2 cut(s) 689, 1512
BslFI GGGAC 2 cut(s) 183, 1566
BslI CCNNNNNNNGG 6 cut(s) 208, 292, 293, 528, 1138, 1713
BsmAI GTCTC 6 cut(s) 151, 229, 426, 429, 447, 687
BsmBI CGTCTC 2 cut(s) 229, 447
BsmFI GGGAC 2 cut(s) 183, 1566
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 2 cut(s) 125, 389
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 38, 1083
Bsp143I GATC 4 cut(s) 39, 183, 375, 1066
BspANI GGCC 2 cut(s) 125, 389
BspCNI CTCAG 5 cut(s) 172, 544, 838, 1201, 1327
BspFNI CGCG 2 cut(s) 369, 440
BspHI TCATGA 1 cut(s) 934
BspLI GGNNCC 3 cut(s) 533, 1447, 1516
BspPI GGATC 1 cut(s) 34
BspT107I GGYRCC 2 cut(s) 1445, 1514
BspTI CTTAAG 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 426
BsrBI CCGCTC 1 cut(s) 564
BsrDI GCAATG 5 cut(s) 830, 1056, 1221, 1227, 1633
BsrFI RCCGGY 1 cut(s) 1511
BsrI ACTGG 2 cut(s) 1477, 1662
BssAI RCCGGY 1 cut(s) 1511
BssECI CCNNGG 2 cut(s) 390, 522
BssMI GATC 4 cut(s) 39, 183, 375, 1066
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 3 cut(s) 162, 1321, 1639
Bst6I CTCTTC 2 cut(s) 1107, 1668
BstAFI CTTAAG 1 cut(s) 788
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstC8I GCNNGC 4 cut(s) 24, 599, 1365, 1700
BstDEI CTNAG 5 cut(s) 180, 552, 846, 1209, 1335
BstF5I GGATG 2 cut(s) 152, 1037
BstFNI CGCG 2 cut(s) 369, 440
BstHHI GCGC 3 cut(s) 925, 1358, 1746
BstKTI GATC 4 cut(s) 42, 186, 378, 1069
BstMAI GTCTC 6 cut(s) 151, 229, 426, 429, 447, 687
BstMBI GATC 4 cut(s) 39, 183, 375, 1066
BstMWI GCNNNNNNNGC 6 cut(s) 23, 94, 1055, 1364, 1418, 1648
BstNI CCWGG 1 cut(s) 731
BstNSI RCATGY 2 cut(s) 700, 980
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 2 cut(s) 688, 729
BstUI CGCG 2 cut(s) 369, 440
BstV1I GCAGC 1 cut(s) 589
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 1066
BstXI CCANNNNNNTGG 1 cut(s) 1591
BstYI RGATCY 1 cut(s) 1066
Bsu36I CCTNAGG 1 cut(s) 552
BsuI GTATCC 1 cut(s) 1225
BsuRI GGCC 2 cut(s) 125, 389
BtgZI GCGATG 1 cut(s) 487
BtsCI GGATG 2 cut(s) 152, 1037
BtsI GCAGTG 5 cut(s) 186, 263, 297, 1308, 1365
BtsIMutI CAGTG 8 cut(s) 186, 263, 297, 762, 843, 1308, 1317, 1365
Cac8I GCNNGC 4 cut(s) 24, 599, 1365, 1700
CciI TCATGA 1 cut(s) 934
CfoI GCGC 3 cut(s) 925, 1358, 1746
Cfr10I RCCGGY 1 cut(s) 1511
Cfr13I GGNCC 3 cut(s) 303, 350, 548
CseI GACGC 2 cut(s) 185, 429
Csp6I GTAC 2 cut(s) 112, 1446
CviQI GTAC 2 cut(s) 112, 1446
DdeI CTNAG 5 cut(s) 180, 552, 846, 1209, 1335
DpnI GATC 4 cut(s) 41, 185, 377, 1068
DpnII GATC 4 cut(s) 39, 183, 375, 1066
DriI GACNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 2 cut(s) 1107, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 2 cut(s) 1107, 1668
Ecl136II GAGCTC 2 cut(s) 36, 1081
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 2 cut(s) 38, 1083
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 3 cut(s) 303, 350, 548
Eco53kI GAGCTC 2 cut(s) 36, 1081
Eco57I CTGAAG 4 cut(s) 110, 188, 1664, 1703
Eco81I CCTNAGG 1 cut(s) 552
EcoICRI GAGCTC 2 cut(s) 36, 1081
EcoRII CCWGG 1 cut(s) 729
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 2 cut(s) 38, 1083
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 2 cut(s) 229, 447
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FaqI GGGAC 2 cut(s) 183, 1566
FauI CCCGC 3 cut(s) 15, 85, 590
Fnu4HI GCNGC 5 cut(s) 73, 95, 575, 578, 1715
FokI GGATG 2 cut(s) 159, 1024
FriOI GRGCYC 2 cut(s) 38, 1083
Fsp4HI GCNGC 5 cut(s) 73, 95, 575, 578, 1715
FspBI CTAG 1 cut(s) 795
FspI TGCGCA 2 cut(s) 924, 1745
GlaI GCGC 3 cut(s) 924, 1357, 1745
GluI GCNGC 5 cut(s) 73, 95, 575, 578, 1715
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 2 cut(s) 175, 1287
HaeIII GGCC 2 cut(s) 125, 389
HapII CCGG 2 cut(s) 689, 1512
HgaI GACGC 2 cut(s) 185, 429
HhaI GCGC 3 cut(s) 925, 1358, 1746
Hin6I GCGC 3 cut(s) 923, 1356, 1744
HinP1I GCGC 3 cut(s) 923, 1356, 1744
HincII GTYRAC 2 cut(s) 676, 892
HindII GTYRAC 2 cut(s) 676, 892
HindIII AAGCTT 2 cut(s) 1380, 1618
HinfI GANTC 6 cut(s) 28, 59, 236, 752, 1004, 1607
HpaI GTTAAC 1 cut(s) 676
HpaII CCGG 2 cut(s) 689, 1512
HphI GGTGA 5 cut(s) 219, 231, 578, 745, 1181
Hpy166II GTNNAC 7 cut(s) 658, 676, 880, 892, 1189, 1599, 1725
Hpy188III TCNNGA 8 cut(s) 379, 551, 935, 1012, 1123, 1266, 1387, 1531
Hpy8I GTNNAC 7 cut(s) 658, 676, 880, 892, 1189, 1599, 1725
HpyAV CCTTC 8 cut(s) 91, 212, 256, 363, 846, 1291, 1537, 1639
HpyCH4III ACNGT 3 cut(s) 162, 1321, 1639
HpyCH4IV ACGT 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 94, 1055, 1364, 1418, 1648
HpyF3I CTNAG 5 cut(s) 180, 552, 846, 1209, 1335
HpySE526I ACGT 1 cut(s) 322
HspAI GCGC 3 cut(s) 923, 1356, 1744
KpnI GGTACC 1 cut(s) 1449
KspAI GTTAAC 1 cut(s) 676
Kzo9I GATC 4 cut(s) 39, 183, 375, 1066
LmnI GCTCC 8 cut(s) 150, 223, 253, 537, 561, 1078, 1086, 1648
Lsp1109I GCAGC 1 cut(s) 589
LweI GCATC 2 cut(s) 910, 1046
MaeI CTAG 1 cut(s) 795
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 2 cut(s) 566, 959
MalI GATC 4 cut(s) 41, 185, 377, 1068
MbiI CCGCTC 1 cut(s) 564
MboI GATC 4 cut(s) 39, 183, 375, 1066
MboII GAAGA 8 cut(s) 245, 761, 872, 955, 1103, 1124, 1616, 1685
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 1066
MhlI GDGCHC 2 cut(s) 38, 1083
MluI ACGCGT 1 cut(s) 438
MlyI GAGTC 2 cut(s) 230, 998
MmeI TCCRAC 1 cut(s) 1242
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 4 cut(s) 468, 675, 789, 867
MslI CAYNNNNRTG 4 cut(s) 807, 1398, 1589, 1739
MspCI CTTAAG 1 cut(s) 788
MspI CCGG 2 cut(s) 689, 1512
MspR9I CCNGG 2 cut(s) 690, 731
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 2 cut(s) 369, 440
MwoI GCNNNNNNNGC 6 cut(s) 23, 94, 1055, 1364, 1418, 1648
NciI CCSGG 1 cut(s) 690
NdeII GATC 4 cut(s) 39, 183, 375, 1066
NlaIV GGNNCC 3 cut(s) 533, 1447, 1516
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 1 cut(s) 566
NsbI TGCGCA 2 cut(s) 924, 1745
NspI RCATGY 2 cut(s) 700, 980
OliI CACNNNNGTG 1 cut(s) 1398
PagI TCATGA 1 cut(s) 934
PciI ACATGT 1 cut(s) 976
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 1021
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 4 cut(s) 28, 59, 752, 1607
PflFI GACNNNGTC 1 cut(s) 497
PkrI GCNGC 5 cut(s) 74, 96, 576, 579, 1716
PleI GAGTC 2 cut(s) 230, 998
PpsI GAGTC 2 cut(s) 230, 998
PscI ACATGT 1 cut(s) 976
PshAI GACNNNNGTC 1 cut(s) 212
Psp124BI GAGCTC 2 cut(s) 38, 1083
Psp6I CCWGG 1 cut(s) 729
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 3 cut(s) 533, 1447, 1516
PspPI GGNCC 3 cut(s) 303, 350, 548
PsuI RGATCY 1 cut(s) 1066
PsyI GACNNNGTC 1 cut(s) 497
RsaI GTAC 2 cut(s) 113, 1447
RsaNI GTAC 2 cut(s) 112, 1446
RseI CAYNNNNRTG 4 cut(s) 807, 1398, 1589, 1739
SacI GAGCTC 2 cut(s) 38, 1083
SaqAI TTAA 4 cut(s) 468, 675, 789, 867
SatI GCNGC 5 cut(s) 73, 95, 575, 578, 1715
Sau3AI GATC 4 cut(s) 39, 183, 375, 1066
Sau96I GGNCC 3 cut(s) 303, 350, 548
SchI GAGTC 2 cut(s) 230, 998
ScrFI CCNGG 2 cut(s) 690, 731
SduI GDGCHC 2 cut(s) 38, 1083
SfaNI GCATC 2 cut(s) 910, 1046
SinI GGWCC 3 cut(s) 303, 350, 548
SmiMI CAYNNNNRTG 4 cut(s) 807, 1398, 1589, 1739
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
SspMI CTAG 1 cut(s) 795
SstI GAGCTC 2 cut(s) 38, 1083
StyD4I CCNGG 2 cut(s) 688, 729
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 162, 1321, 1639
TaiI ACGT 1 cut(s) 325
TaqI TCGA 1 cut(s) 38
TauI GCSGC 4 cut(s) 75, 97, 577, 1717
TfiI GAWTC 4 cut(s) 28, 59, 752, 1607
Tru1I TTAA 4 cut(s) 468, 675, 789, 867
Tru9I TTAA 4 cut(s) 468, 675, 789, 867
TscAI CASTG 8 cut(s) 193, 263, 297, 762, 850, 1308, 1324, 1365
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 1 cut(s) 577
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 6 cut(s) 416, 431, 897, 923, 951, 1468
TspGWI ACGGA 2 cut(s) 224, 1567
TspRI CASTG 8 cut(s) 193, 263, 297, 762, 850, 1308, 1324, 1365
Tth111I GACNNNGTC 1 cut(s) 497
Vha464I CTTAAG 1 cut(s) 788
VpaK11BI GGWCC 3 cut(s) 303, 350, 548
XapI RAATTY 3 cut(s) 605, 869, 1015
XceI RCATGY 2 cut(s) 700, 980
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.