pycom07g25700

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015479
Physical Location & Seq
Forward (+)
25927134 .. 25929720
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25700.7

Sequence Viewer

Length: 1224 bp
ATGCTTGATACCAAGGGTCCTGAGGTTAGGAGCGGTGACCTGCCACAGTCAATCAATTTAGAAAGTGGGCAGGAATTTACCTTTACAATTAAAAGAGGGGTTGGCACATCAGATTGTGTTAGCGTGAACTATGATGATTTCGTTAATGATGTAGCACCGGGAGACATGCTTCTGGTTGATGGTGGTATGATGTCATTTCTGGTGAAGTCCAAGACAGAAGAATCAGTGAAATGTGAAGTTGTTGATGGAGGAGAGCTTAAGTCTAGGCGACATTTGAATGTGCGAGGGAAAAGTGCAACATTGCCTTCTATCACTGAGAAAGATTGGGAAGATATTAAATTTGGAGTGGACAATAAAGTTGACTTCTACGCTGTTTCATTTGTCAAAGATGCGCAAGTTGTTCATGAATTGAAGAATTATCTGCAAAGTAATAATGCAGATATACACGTTATTGTAAAAATAGAAAGTGCAGACTCTATCCCGAATTTGCATTCGATTATCACAGCATCCGATGGGGCAATGGTTGCAAGAGGAGATCTTGGTGCAGAGCTCCCTGTTGAGGAAGTTCCGCTTTTGCAGGAAGAGATAATCAGGATATGCCGTAGCATGGGAAAGGCTGTTATTGTGGCAACAAATATGCTGGAAAGCATGATTGTTCACCCAACACCAACCAGAGCTGAGGTATCCGACATTGCCATTGCTGTTAGAGAGGGTTCTGATGGAATTATGCTTTCTGGAGAGACTGCTCATGGAAAGTATCCTTTGAAGGCAGTGAAAGTTATGCACACTGTCGCACTTCGGACTGAGGCGACCATCTTGGGTGGCGCGGTGCCAGCCAATCTTGGTAAAGCTTTCAAGAACCACATGAGTGAGATGTTTGCTTACCATGCAACCATGATGTCCAATACTCTTGGTACCTCAATCGTTGTCTTTACCAGAACTGGTTTCATGGCTGTTCTATTGAGCCATTACCGACCTACTGGGACCATATTTGCCTTTACGAATGATAAAAGCATACAACGGAGGTTGGCTTTGTATCAAGGCGTATGTCCCATATACATGGAGTTCACAGAAGATTCTGAAACAAGCTTTAGCAATGCCTTGACTGTGCTGAAGAAGCAAGGATTGGTAAAGGCAGGAGAAGAGGTCGCACTCGTTCAAAGTGGCAGGCAACCCATTTGGCGGCTCCAATCCACTCACAACATTCAAGTGCGCAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

408

Amino Acids

44.54

Weight (kDa)

5.92

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52920
fragaria_vesca FvH4_7g30750
malus_domestica MD01G1212800.v1.1 MD07G1282700.v1.1
prunus_persica Prupe.2G305000_v2.0.a1
pyrus_communis pycom01g22390 pycom07g25700
rosa_chinensis RchiOBHm_Chr1g0379701
rosa_laevigata RLG00000026366
rosa_multiflora Rmu_sc0013028.1_g000007
rosa_rugosa Rorug01G0414700
rosa_samantha Rh1AG438100 Rh1BG394600 Rh1CG407600 Rh1DG425000
rosa_wichuraiana Rw1G038180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 393, 1214
Acc36I ACCTGC 1 cut(s) 48
Acc65I GGTACC 1 cut(s) 914
AccB1I GGYRCC 2 cut(s) 829, 914
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 1 cut(s) 827
AciI CCGC 4 cut(s) 33, 569, 827, 1183
AcsI RAATTY 4 cut(s) 74, 338, 484, 1218
AcuI CTGAAG 1 cut(s) 1133
AfaI GTAC 1 cut(s) 916
AfiI CCNNNNNNNGG 3 cut(s) 559, 607, 1182
AflII CTTAAG 1 cut(s) 257
AflIII ACRYGT 1 cut(s) 445
AgsI TTSAA 6 cut(s) 277, 412, 766, 856, 1160, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 1109, 1141
AleI CACNNNNGTG 1 cut(s) 867
AluBI AGCT 5 cut(s) 256, 550, 677, 851, 1089
AluI AGCT 5 cut(s) 256, 550, 677, 851, 1089
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 2 cut(s) 156, 734
ApoI RAATTY 4 cut(s) 74, 338, 484, 1218
Asp718I GGTACC 1 cut(s) 914
AspLEI GCGC 3 cut(s) 394, 827, 1215
AspS9I GGNCC 2 cut(s) 17, 984
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 3 cut(s) 47, 214, 650
AvaII GGWCC 2 cut(s) 17, 984
AxyI CCTNAGG 1 cut(s) 21
BanI GGYRCC 2 cut(s) 829, 914
BanII GRGCYC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 552
BbvCI CCTCAGC 1 cut(s) 678
BccI CCATC 5 cut(s) 173, 239, 506, 713, 821
BceAI ACGGC 1 cut(s) 585
BciVI GTATCC 2 cut(s) 694, 768
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 156, 734
BfaI CTAG 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 48
BfuI GTATCC 2 cut(s) 694, 768
BglII AGATCT 1 cut(s) 535
BisI GCNGC 1 cut(s) 1184
BlsI GCNGC 1 cut(s) 1185
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 2 cut(s) 17, 984
BmgT120I GGNCC 2 cut(s) 17, 984
BmiI GGNNCC 5 cut(s) 18, 831, 916, 985, 1187
BmrFI CCNGG 1 cut(s) 159
BmrI ACTGGG 1 cut(s) 990
BmsI GCATC 2 cut(s) 379, 515
BmuI ACTGGG 1 cut(s) 990
BpmI CTGGAG 1 cut(s) 756
Bpu10I CCTNAGC 1 cut(s) 678
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 12
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 607, 1182
Bse1I ACTGG 2 cut(s) 946, 985
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 5 cut(s) 299, 525, 690, 696, 1102
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 506
BseLI CCNNNNNNNGG 3 cut(s) 559, 607, 1182
BseMI GCAATG 5 cut(s) 299, 525, 690, 696, 1102
BseMII CTCAG 4 cut(s) 12, 306, 669, 795
BseNI ACTGG 2 cut(s) 946, 985
BseRI GAGGAG 2 cut(s) 264, 546
BsgI GTGCAG 2 cut(s) 489, 564
Bsh1236I CGCG 1 cut(s) 827
BshNI GGYRCC 2 cut(s) 829, 914
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 2 cut(s) 997, 1035
BslI CCNNNNNNNGG 3 cut(s) 559, 607, 1182
BsmAI GTCTC 2 cut(s) 156, 734
BsmFI GGGAC 2 cut(s) 997, 1035
BsmI GAATGC 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 1 cut(s) 535
BspACI CCGC 4 cut(s) 33, 569, 827, 1183
BspCNI CTCAG 4 cut(s) 13, 307, 670, 796
BspFNI CGCG 1 cut(s) 827
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 5 cut(s) 18, 831, 916, 985, 1187
BspMI ACCTGC 1 cut(s) 48
BspT107I GGYRCC 2 cut(s) 829, 914
BspTI CTTAAG 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 5 cut(s) 299, 525, 690, 696, 1102
BsrI ACTGG 2 cut(s) 946, 985
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 535
BssT1I CCWWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 48, 790, 1108
Bst6I CTCTTC 2 cut(s) 576, 1137
BstAFI CTTAAG 1 cut(s) 257
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 2 cut(s) 834, 1169
BstDEI CTNAG 4 cut(s) 21, 315, 678, 804
BstEII GGTNACC 1 cut(s) 35
BstF5I GGATG 1 cut(s) 506
BstFNI CGCG 1 cut(s) 827
BstHHI GCGC 3 cut(s) 394, 827, 1215
BstKTI GATC 1 cut(s) 538
BstMAI GTCTC 2 cut(s) 156, 734
BstMBI GATC 1 cut(s) 535
BstMWI GCNNNNNNNGC 4 cut(s) 524, 833, 887, 1117
BstNSI RCATGY 1 cut(s) 169
BstPI GGTNACC 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 157
BstUI CGCG 1 cut(s) 827
BstX2I RGATCY 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 535
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 2 cut(s) 694, 768
BtsCI GGATG 1 cut(s) 506
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 4 cut(s) 231, 312, 777, 786
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 2 cut(s) 834, 1169
CciI TCATGA 1 cut(s) 403
CfoI GCGC 3 cut(s) 394, 827, 1215
Cfr13I GGNCC 2 cut(s) 17, 984
Csp6I GTAC 1 cut(s) 915
CviQI GTAC 1 cut(s) 915
DdeI CTNAG 4 cut(s) 21, 315, 678, 804
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 576, 1137
EarI CTCTTC 2 cut(s) 576, 1137
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 552
Eco47I GGWCC 2 cut(s) 17, 984
Eco53kI GAGCTC 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 1133
Eco81I CCTNAGG 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 35
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FaqI GGGAC 2 cut(s) 997, 1035
Fnu4HI GCNGC 1 cut(s) 1184
FokI GGATG 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 1 cut(s) 1184
FspBI CTAG 1 cut(s) 264
FspI TGCGCA 2 cut(s) 393, 1214
GlaI GCGC 3 cut(s) 393, 826, 1214
GluI GCNGC 1 cut(s) 1184
GsuI CTGGAG 1 cut(s) 756
HapII CCGG 1 cut(s) 158
HhaI GCGC 3 cut(s) 394, 827, 1215
Hin6I GCGC 3 cut(s) 392, 825, 1213
HinP1I GCGC 3 cut(s) 392, 825, 1213
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 849, 1087
HinfI GANTC 3 cut(s) 221, 473, 1076
HpaII CCGG 1 cut(s) 158
HphI GGTGA 3 cut(s) 47, 214, 650
Hpy166II GTNNAC 5 cut(s) 127, 349, 361, 658, 1068
Hpy188I TCNGA 6 cut(s) 112, 511, 688, 718, 801, 1081
Hpy188III TCNNGA 6 cut(s) 20, 404, 481, 592, 735, 856
Hpy8I GTNNAC 5 cut(s) 127, 349, 361, 658, 1068
HpyAV CCTTC 2 cut(s) 315, 760
HpyCH4III ACNGT 3 cut(s) 48, 790, 1108
HpyCH4IV ACGT 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 4 cut(s) 524, 833, 887, 1117
HpyF3I CTNAG 4 cut(s) 21, 315, 678, 804
HpySE526I ACGT 1 cut(s) 447
HspAI GCGC 3 cut(s) 392, 825, 1213
KpnI GGTACC 1 cut(s) 918
Kzo9I GATC 1 cut(s) 535
LmnI GCTCC 3 cut(s) 30, 555, 1191
LweI GCATC 2 cut(s) 379, 515
MaeI CTAG 1 cut(s) 264
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 35
MalI GATC 1 cut(s) 537
MbiI CCGCTC 1 cut(s) 33
MboI GATC 1 cut(s) 535
MboII GAAGA 7 cut(s) 230, 341, 424, 593, 1085, 1126, 1154
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 9 cut(s) 55, 74, 87, 338, 407, 415, 484, 723, 1218
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 711
MseI TTAA 4 cut(s) 90, 144, 258, 336
MslI CAYNNNNRTG 4 cut(s) 276, 867, 1058, 1208
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 490
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 4 cut(s) 524, 833, 887, 1117
NciI CCSGG 1 cut(s) 159
NdeII GATC 1 cut(s) 535
NlaIV GGNNCC 5 cut(s) 18, 831, 916, 985, 1187
NmuCI GTSAC 1 cut(s) 35
NsbI TGCGCA 2 cut(s) 393, 1214
NspI RCATGY 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 867
PagI TCATGA 1 cut(s) 403
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 221, 1076
PkrI GCNGC 1 cut(s) 1185
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 17
PspEI GGTNACC 1 cut(s) 35
PspN4I GGNNCC 5 cut(s) 18, 831, 916, 985, 1187
PspPI GGNCC 2 cut(s) 17, 984
PspPPI RGGWCCY 1 cut(s) 17
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 916
RsaNI GTAC 1 cut(s) 915
RseI CAYNNNNRTG 4 cut(s) 276, 867, 1058, 1208
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 4 cut(s) 90, 144, 258, 336
SatI GCNGC 1 cut(s) 1184
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 2 cut(s) 17, 984
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 2 cut(s) 379, 515
SinI GGWCC 2 cut(s) 17, 984
SmiMI CAYNNNNRTG 4 cut(s) 276, 867, 1058, 1208
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 9 cut(s) 55, 74, 87, 338, 407, 415, 484, 723, 1218
SsiI CCGC 4 cut(s) 33, 569, 827, 1183
SspMI CTAG 1 cut(s) 264
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 48, 790, 1108
TaiI ACGT 1 cut(s) 450
TaqI TCGA 1 cut(s) 494
TasI AATT 9 cut(s) 55, 74, 87, 338, 407, 415, 484, 723, 1218
TauI GCSGC 1 cut(s) 1186
TfiI GAWTC 2 cut(s) 221, 1076
Tru1I TTAA 4 cut(s) 90, 144, 258, 336
Tru9I TTAA 4 cut(s) 90, 144, 258, 336
TscAI CASTG 4 cut(s) 231, 319, 777, 793
TseFI GTSAC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 4 cut(s) 366, 392, 420, 937
TspGWI ACGGA 1 cut(s) 1036
TspRI CASTG 4 cut(s) 231, 319, 777, 793
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 17, 984
XapI RAATTY 4 cut(s) 74, 338, 484, 1218
XceI RCATGY 1 cut(s) 169
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.