Rh1DG425000

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015479
Physical Location & Seq
Forward (+)
63884089 .. 63887976
3888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG425000.1

Sequence Viewer

Length: 1530 bp
ATGGCTCAGGTGGTGGCTATGAGAGCGATTCAGAGCTCGATCCTGTGCCCCGGGTCCGGATCCGCCCAGGGACGGAGCTCCGATAAGCTCAAGCCGCCGAGCTTCGCTTCCAGAGTTCTGGCGAATGAGGAGAAGAAGAGCACCAGCCGGAAAGTGGTCGGCAGCAGAAGGATGAGGTCTGAGATCGCCGCCAAGCGGCCGTTACAGACCGAGGTCGTTCCGATGTCGCCCGAGGACTTGCCCAAGATAGAGGAGCAATTCCAGCAATTACAGACGATTCAGCAATATGGTGACACTTCGGTGGGTATGTGGACAAAGCCAACAGTGAGACGTAAGACCAAGATTGTATGCACAATTGGTCCTTCCACCAACACTCGTGAAATGATCTGGAAGTTGGCTGAGGCGGGGATGAATGTTGCGCGGATGAATATGTCCCACGGAGATCATGCGTCTCATCAGAAGGTTATCGACTTGGTTAAGGAATATAATGAGCAATCCAAGGACAATGTCATTGCAATCATGCTTGATACCAAGGGCCCTGAGGTGAGGAGTGGTGACCTGCCCCAGCCAATTAATTTGGCCCCTGGGCAGGAATTTACTTTTACAATTCAGAGAGGGGTTGGAACAGCAGAGTGTGTTAGTGTGAACTATGATGATTTTGTTAATGATGTGGAAGTGGGTGACATGCTTCTGGTTGATGGTGGTATGATGTCATTTTTGGTGAAGTCCAAGACAGCAGACTCAGTGAAATGTGAAGTTGTCGATGGAGGAGAACTTAAGTCTAGGCGGCACTTGAATGTGCGAGGGAAAAGTGCAACATTGGCTTCTATCACTGAGAAAGATTGGGAAGATATCAAATTTGGAGTGGATAATAAAGTTGACTTCTATGCAGTTTCGTTTGTTAAAGATGCAGAAGTTGTTCATGAATTGAAGAATTATCTGCGAAGCTGTAATGCGGATATACATGTTATTGTAAAAATTGAAAGTGCAGACTCGATCCCAAATTTGCATTCGATTATCACAGCATCTGATGGGGCCATGGTTGCAAGAGGAGATCTAGGTGCAGAACTCCCTGTTGAAGAGGTCCCACTTCTGCAGGAAGAGATAATCAGGCTATGCCGTAGCATGGGGAAGGCTGTTATTGTTGCAACAAATATGCTTGAGAGCATGATTGTTCACCCAACCCCAACCAGAGCTGAGGTATCAGACATTGCCATTGCTGTCCGGGAGGGTGCTGATGCAGTTATGCTGTCGGGAGAAACTGCTAATGGAAAGTATCCGCTGAAAGCCGTGAAAGTTATGCACACTGTTGCTCTGCGGACAGAGGCAACCGTCACAGGTGGCCAAATGCCAGCCAATCTTGGCAAAGCTTTTAAGAATCATATGAGCGAGATGTTTGCTTACCATGCAACCATGATGTCCAATACTCTTGGAACCTCAATCGTCGTGTTCACTAGATCTGGTTTCATGGCTATTCTATTGAGCCATTTTCGTCCTTCTGGGACAATATTTGCTTTTACAAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

509

Amino Acids

55.64

Weight (kDa)

6.66

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 111 - 431 1.2e-111 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52920
fragaria_vesca FvH4_7g30750
malus_domestica MD01G1212800.v1.1 MD07G1282700.v1.1
prunus_persica Prupe.2G305000_v2.0.a1
pyrus_communis pycom01g22390 pycom07g25700
rosa_chinensis RchiOBHm_Chr1g0379701
rosa_laevigata RLG00000026366
rosa_multiflora Rmu_sc0013028.1_g000007
rosa_rugosa Rorug01G0414700
rosa_samantha Rh1AG438100 Rh1BG394600 Rh1CG407600 Rh1DG425000
rosa_wichuraiana Rw1G038180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 567
AccII CGCG 1 cut(s) 421
AccIII TCCGGA 1 cut(s) 56
AclWI GGATC 4 cut(s) 34, 54, 67, 991
AcoI YGGCCR 2 cut(s) 197, 1342
AcsI RAATTY 3 cut(s) 593, 857, 1003
AfiI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 589, 1126
AflII CTTAAG 1 cut(s) 776
AflIII ACRYGT 1 cut(s) 964
AgsI TTSAA 4 cut(s) 796, 931, 983, 1079
AjnI CCWGG 2 cut(s) 66, 583
AjuI GAANNNNNNNTTGG 2 cut(s) 994, 1026
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 7 cut(s) 36, 78, 88, 102, 948, 1196, 1370
AluI AGCT 7 cut(s) 36, 78, 88, 102, 948, 1196, 1370
Alw21I GWGCWC 3 cut(s) 38, 80, 143
Alw26I GTCTC 2 cut(s) 322, 456
AlwI GGATC 4 cut(s) 34, 54, 67, 991
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 2 cut(s) 50, 230
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 5 cut(s) 197, 535, 579, 1035, 1342
ApaI GGGCCC 1 cut(s) 539
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 3 cut(s) 593, 857, 1003
AseI ATTAAT 1 cut(s) 573
Asp700I GAANNNNTTC 1 cut(s) 918
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 7 cut(s) 54, 359, 535, 536, 580, 1035, 1084
AsuC2I CCSGG 3 cut(s) 51, 52, 1226
AsuHPI GGTGA 6 cut(s) 302, 556, 566, 692, 733, 1169
AvaI CYCGRG 2 cut(s) 50, 230
AvaII GGWCC 3 cut(s) 54, 359, 1084
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 2 cut(s) 50, 539
BalI TGGCCA 1 cut(s) 1344
BamHI GGATCC 1 cut(s) 59
BanII GRGCYC 3 cut(s) 38, 80, 539
BauI CACGAG 1 cut(s) 375
Bbv12I GWGCWC 3 cut(s) 38, 80, 143
BbvCI CCTCAGC 2 cut(s) 399, 1197
BbvI GCAGC 1 cut(s) 174
BccI CCATC 3 cut(s) 692, 758, 1025
BceAI ACGGC 3 cut(s) 184, 1104, 1274
BcgI CGANNNNNNTGC 2 cut(s) 1379, 1413
BciT130I CCWGG 2 cut(s) 68, 585
BciVI GTATCC 1 cut(s) 1287
BcnI CCSGG 3 cut(s) 51, 52, 1226
BcoDI GTCTC 2 cut(s) 322, 456
BfaI CTAG 3 cut(s) 783, 1058, 1455
BfmI CTRYAG 1 cut(s) 1094
BfrI CTTAAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 1 cut(s) 1287
BglII AGATCT 2 cut(s) 1054, 1457
BisI GCNGC 5 cut(s) 95, 163, 189, 197, 788
BlsI GCNGC 5 cut(s) 96, 164, 190, 198, 789
Bme1390I CCNGG 5 cut(s) 51, 52, 68, 585, 1226
Bme18I GGWCC 3 cut(s) 54, 359, 1084
BmeT110I CYCGRG 2 cut(s) 50, 230
BmgT120I GGNCC 7 cut(s) 54, 359, 535, 536, 580, 1035, 1084
BmiI GGNNCC 7 cut(s) 55, 61, 537, 582, 1036, 1086, 1435
BmrFI CCNGG 5 cut(s) 51, 52, 68, 585, 1226
BmsI GCATC 3 cut(s) 898, 1034, 1228
BoxI GACNNNNGTC 1 cut(s) 212
Bpu10I CCTNAGC 3 cut(s) 6, 399, 1197
BpuEI CTTGAG 2 cut(s) 74, 1181
BpuMI CCSGG 3 cut(s) 51, 52, 1226
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 589, 1126
Bse21I CCTNAGG 1 cut(s) 540
Bse3DI GCAATG 3 cut(s) 510, 1209, 1215
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 2 cut(s) 68, 585
BseGI GGATG 3 cut(s) 177, 414, 429
BseLI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 589, 1126
BseMI GCAATG 3 cut(s) 510, 1209, 1215
BseMII CTCAG 7 cut(s) 20, 171, 390, 531, 756, 825, 1188
BseRI GAGGAG 5 cut(s) 143, 266, 562, 783, 1065
BseSI GKGCMC 2 cut(s) 50, 539
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 174
BseYI CCCAGC 1 cut(s) 564
BsgI GTGCAG 2 cut(s) 1008, 1083
Bsh1236I CGCG 1 cut(s) 421
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 5 cut(s) 199, 537, 581, 1037, 1344
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 3 cut(s) 38, 80, 143
BsiHKCI CYCGRG 2 cut(s) 50, 230
BsiSI CCGG 4 cut(s) 51, 57, 148, 1225
BslFI GGGAC 4 cut(s) 84, 418, 1070, 1516
BslI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 589, 1126
BsmAI GTCTC 2 cut(s) 322, 456
BsmBI CGTCTC 2 cut(s) 322, 456
BsmFI GGGAC 4 cut(s) 84, 418, 1070, 1516
BsmI GAATGC 1 cut(s) 1009
BsnI GGCC 5 cut(s) 199, 537, 581, 1037, 1344
BsoBI CYCGRG 2 cut(s) 50, 230
Bsp120I GGGCCC 1 cut(s) 535
Bsp1286I GDGCHC 5 cut(s) 38, 50, 80, 143, 539
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
Bsp19I CCATGG 1 cut(s) 1038
BspANI GGCC 5 cut(s) 199, 537, 581, 1037, 1344
BspCNI CTCAG 7 cut(s) 19, 172, 391, 532, 755, 826, 1189
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 1 cut(s) 421
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 7 cut(s) 55, 61, 537, 582, 1036, 1086, 1435
BspMAI CTGCAG 1 cut(s) 1098
BspMI ACCTGC 1 cut(s) 567
BspPI GGATC 4 cut(s) 34, 54, 67, 991
BspQI GCTCTTC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 3 cut(s) 510, 1209, 1215
BssMI GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 3 cut(s) 498, 531, 1038
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 68, 585
Bst4CI ACNGT 3 cut(s) 325, 1309, 1333
Bst6I CTCTTC 3 cut(s) 131, 1074, 1095
BstAFI CTTAAG 1 cut(s) 776
BstC8I GCNNGC 1 cut(s) 1353
BstDEI CTNAG 7 cut(s) 6, 180, 399, 540, 742, 834, 1197
BstDSI CCRYGG 2 cut(s) 436, 1038
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 3 cut(s) 177, 414, 429
BstFNI CGCG 1 cut(s) 421
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 8 cut(s) 42, 62, 186, 387, 445, 999, 1057, 1460
BstMAI GTCTC 2 cut(s) 322, 456
BstMBI GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 6 cut(s) 23, 94, 262, 821, 1043, 1406
BstNI CCWGG 2 cut(s) 68, 585
BstNSI RCATGY 2 cut(s) 688, 968
BstPAI GACNNNNGTC 1 cut(s) 212
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 5 cut(s) 49, 50, 66, 583, 1224
BstSFI CTRYAG 1 cut(s) 1094
BstSLI GKGCMC 2 cut(s) 50, 539
BstUI CGCG 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 174
BstX2I RGATCY 3 cut(s) 59, 1054, 1457
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 3 cut(s) 59, 1054, 1457
BstZI CGGCCG 1 cut(s) 197
Bsu36I CCTNAGG 1 cut(s) 540
BsuI GTATCC 1 cut(s) 1287
BsuRI GGCC 5 cut(s) 199, 537, 581, 1037, 1344
BtgI CCRYGG 2 cut(s) 436, 1038
BtsCI GGATG 3 cut(s) 177, 414, 429
BtsIMutI CAGTG 4 cut(s) 330, 750, 831, 1305
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 1 cut(s) 1353
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 922
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 7 cut(s) 54, 359, 535, 536, 580, 1035, 1084
Cfr9I CCCGGG 1 cut(s) 50
CseI GACGC 1 cut(s) 438
DdeI CTNAG 7 cut(s) 6, 180, 399, 540, 742, 834, 1197
DpnI GATC 8 cut(s) 41, 61, 185, 386, 444, 998, 1056, 1459
DpnII GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
EaeI YGGCCR 2 cut(s) 197, 1342
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 131, 1074, 1095
EarI CTCTTC 3 cut(s) 131, 1074, 1095
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 2 cut(s) 36, 78
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 3 cut(s) 498, 531, 1038
Eco24I GRGCYC 3 cut(s) 38, 80, 539
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 3 cut(s) 54, 359, 1084
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 2 cut(s) 36, 78
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 2 cut(s) 50, 230
Eco91I GGTNACC 1 cut(s) 554
EcoICRI GAGCTC 2 cut(s) 36, 78
EcoO109I RGGNCCY 3 cut(s) 535, 536, 1084
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 2 cut(s) 66, 583
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 3 cut(s) 498, 531, 1038
EcoT38I GRGCYC 3 cut(s) 38, 80, 539
ErhI CCWWGG 3 cut(s) 498, 531, 1038
Esp3I CGTCTC 2 cut(s) 322, 456
FaqI GGGAC 4 cut(s) 84, 418, 1070, 1516
FauI CCCGC 1 cut(s) 397
FauNDI CATATG 1 cut(s) 1383
Fnu4HI GCNGC 5 cut(s) 95, 163, 189, 197, 788
FokI GGATG 3 cut(s) 184, 421, 436
FriOI GRGCYC 3 cut(s) 38, 80, 539
Fsp4HI GCNGC 5 cut(s) 95, 163, 189, 197, 788
FspBI CTAG 3 cut(s) 783, 1058, 1455
GlaI GCGC 1 cut(s) 420
GluI GCNGC 5 cut(s) 95, 163, 189, 197, 788
GsaI CCCAGC 1 cut(s) 568
HaeIII GGCC 5 cut(s) 199, 537, 581, 1037, 1344
HapII CCGG 4 cut(s) 51, 57, 148, 1225
HgaI GACGC 1 cut(s) 438
HhaI GCGC 1 cut(s) 421
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 1368
HinfI GANTC 5 cut(s) 28, 277, 740, 992, 1378
HpaII CCGG 4 cut(s) 51, 57, 148, 1225
HphI GGTGA 6 cut(s) 302, 556, 566, 692, 733, 1169
Hpy166II GTNNAC 5 cut(s) 312, 646, 880, 1177, 1452
Hpy188I TCNGA 8 cut(s) 33, 82, 181, 222, 459, 612, 1030, 1207
Hpy188III TCNNGA 6 cut(s) 57, 111, 377, 388, 923, 1254
Hpy8I GTNNAC 5 cut(s) 312, 646, 880, 1177, 1452
Hpy99I CGWCG 1 cut(s) 1448
HpyAV CCTTC 5 cut(s) 162, 372, 454, 1126, 1506
HpyCH4III ACNGT 3 cut(s) 325, 1309, 1333
HpyCH4IV ACGT 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 94, 262, 821, 1043, 1406
HpyF3I CTNAG 7 cut(s) 6, 180, 399, 540, 742, 834, 1197
HpySE526I ACGT 1 cut(s) 331
HspAI GCGC 1 cut(s) 419
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 75, 83, 253
Lsp1109I GCAGC 1 cut(s) 174
LweI GCATC 3 cut(s) 898, 1034, 1228
MaeI CTAG 3 cut(s) 783, 1058, 1455
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 5 cut(s) 201, 290, 554, 680, 1333
MalI GATC 8 cut(s) 41, 61, 185, 386, 444, 998, 1056, 1459
MboI GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
MboII GAAGA 6 cut(s) 145, 148, 860, 943, 1091, 1112
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 3 cut(s) 59, 1054, 1457
MhlI GDGCHC 5 cut(s) 38, 50, 80, 143, 539
MlsI TGGCCA 1 cut(s) 1344
MluNI TGGCCA 1 cut(s) 1344
MlyI GAGTC 2 cut(s) 734, 986
MmeI TCCRAC 1 cut(s) 601
Mox20I TGGCCA 1 cut(s) 1344
MroI TCCGGA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 918
MscI TGGCCA 1 cut(s) 1344
MseI TTAA 6 cut(s) 477, 573, 663, 777, 903, 1374
MslI CAYNNNNRTG 2 cut(s) 299, 795
Msp20I TGGCCA 1 cut(s) 1344
MspA1I CMGCKG 1 cut(s) 1282
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 4 cut(s) 51, 57, 148, 1225
MspR9I CCNGG 5 cut(s) 51, 52, 68, 585, 1226
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 1009
MvaI CCWGG 2 cut(s) 68, 585
MvnI CGCG 1 cut(s) 421
MwoI GCNNNNNNNGC 6 cut(s) 23, 94, 262, 821, 1043, 1406
NciI CCSGG 3 cut(s) 51, 52, 1226
NcoI CCATGG 1 cut(s) 1038
NdeI CATATG 1 cut(s) 1383
NdeII GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
NlaIV GGNNCC 7 cut(s) 55, 61, 537, 582, 1036, 1086, 1435
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 4 cut(s) 290, 554, 680, 1333
NspI RCATGY 2 cut(s) 688, 968
OliI CACNNNNGTG 1 cut(s) 299
PagI TCATGA 1 cut(s) 922
PasI CCCWGGG 2 cut(s) 67, 584
PciI ACATGT 1 cut(s) 964
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 1 cut(s) 1009
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 3 cut(s) 28, 277, 1378
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 1224
PkrI GCNGC 5 cut(s) 96, 164, 190, 198, 789
PleI GAGTC 2 cut(s) 734, 986
PpsI GAGTC 2 cut(s) 734, 986
PpuMI RGGWCCY 1 cut(s) 1084
PscI ACATGT 1 cut(s) 964
PshAI GACNNNNGTC 1 cut(s) 212
PshBI ATTAAT 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 38, 80
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 2 cut(s) 66, 583
PspEI GGTNACC 1 cut(s) 554
PspFI CCCAGC 1 cut(s) 564
PspGI CCWGG 2 cut(s) 66, 583
PspN4I GGNNCC 7 cut(s) 55, 61, 537, 582, 1036, 1086, 1435
PspOMI GGGCCC 1 cut(s) 535
PspPI GGNCC 7 cut(s) 54, 359, 535, 536, 580, 1035, 1084
PspPPI RGGWCCY 1 cut(s) 1084
PstI CTGCAG 1 cut(s) 1098
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 3 cut(s) 59, 1054, 1457
PsyI GACNNNGTC 1 cut(s) 506
RseI CAYNNNNRTG 2 cut(s) 299, 795
SacI GAGCTC 2 cut(s) 38, 80
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 6 cut(s) 477, 573, 663, 777, 903, 1374
SatI GCNGC 5 cut(s) 95, 163, 189, 197, 788
Sau3AI GATC 8 cut(s) 39, 59, 183, 384, 442, 996, 1054, 1457
Sau96I GGNCC 7 cut(s) 54, 359, 535, 536, 580, 1035, 1084
SchI GAGTC 2 cut(s) 734, 986
ScrFI CCNGG 5 cut(s) 51, 52, 68, 585, 1226
SduI GDGCHC 5 cut(s) 38, 50, 80, 143, 539
SfaNI GCATC 3 cut(s) 898, 1034, 1228
SfcI CTRYAG 1 cut(s) 1094
SinI GGWCC 3 cut(s) 54, 359, 1084
SmaI CCCGGG 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 299, 795
SmlI CTYRAG 3 cut(s) 89, 776, 1160
SmoI CTYRAG 3 cut(s) 89, 776, 1160
SspI AATATT 1 cut(s) 1509
SspMI CTAG 3 cut(s) 783, 1058, 1455
SstI GAGCTC 2 cut(s) 38, 80
StyD4I CCNGG 5 cut(s) 49, 50, 66, 583, 1224
StyI CCWWGG 3 cut(s) 498, 531, 1038
TaaI ACNGT 3 cut(s) 325, 1309, 1333
TaiI ACGT 1 cut(s) 334
TaqI TCGA 5 cut(s) 38, 468, 762, 995, 1013
TaqII GACCGA 1 cut(s) 224
TauI GCSGC 4 cut(s) 97, 191, 199, 790
TfiI GAWTC 3 cut(s) 28, 277, 1378
Tru1I TTAA 6 cut(s) 477, 573, 663, 777, 903, 1374
Tru9I TTAA 6 cut(s) 477, 573, 663, 777, 903, 1374
TscAI CASTG 4 cut(s) 330, 750, 838, 1312
TseFI GTSAC 4 cut(s) 290, 554, 680, 1333
TseI GCWGC 1 cut(s) 162
Tsp45I GTSAC 4 cut(s) 290, 554, 680, 1333
TspDTI ATGAA 5 cut(s) 425, 440, 911, 939, 1456
TspGWI ACGGA 2 cut(s) 88, 453
TspMI CCCGGG 1 cut(s) 50
TspRI CASTG 4 cut(s) 330, 750, 838, 1312
Tth111I GACNNNGTC 1 cut(s) 506
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 54, 359, 1084
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 3 cut(s) 593, 857, 1003
XceI RCATGY 2 cut(s) 688, 968
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmaI CCCGGG 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 3 cut(s) 783, 1058, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.