Rw1G038180

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015479
Physical Location & Seq
Forward (+)
65784524 .. 65787972
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038180.1

Sequence Viewer

Length: 1707 bp
ATGGCTCAGGTGGTGGCTATGAGAGCGATTCAGAGCTCGATCCTGCGCCCCGGGTCCGGATCCGCCCAGGGACGGAGCTCCGATAAGCTCAAGCCGCCGAGCTTCGCTTCCAGAGTTCTGGCGAATGAGGAGAAGAAGAGCACCAGCCGGAAAGTGGTCGGCAGCAGAAGGATGAGGTCTGAGATCGCCGCCAAGCGGCCGTTACAGACCGAGGTCGTTCCCATGTCGCCCGAGGACTTGCCCAAGCAATTCCAGCAATTACAGACGATTCAGCAATATGGTGACACTTCAGTGGGTATGTGGACAAAGCCAACAGTGAGACGTAAGACCAAGATTGTATGCACAATTGGTCCTTCCACCAACACTCGTGAAATGATCTGGAAGTTGGCTGAGGCGGGGATGAATGTTGCGCGGATGAATATGTCCCATGGAGATCATGCGTCTCATCAGAAGGTTATCGACTTGGTTAAGGAATATAATGAGCAATCCAAGGACAATGTCATTGCAATCATGCTTGATACCAAGGGCCCTGAGGTTAGGAGTGGTGACCTGCCCCAGCCAATTAATTTGGCCCCTGGGCAGGAATTTACTTTTACAATTCAGAGAGGGGTTGGAACAGCAGAGTGTGTTAGTGTGAACTATGATGATTTTGTTAATGATGTGGAAGTGGGTGACATGCTTCTGGTTGATGGTGGTATGATGTCATTTTTGGTGAAGTCCAAGACAGCAGACTCAGTGAAATGTGAAGTTGTCGATGGAGGAGAACTTAAGTCTAGGCGGCACTTGAATGTGCGAGGGAAAAGTGCAACATTGGCTTCTATCACTGAGAAAGATTGGGAAGATATCAAATTTGGAGTGGATAATAAAGTTGACTTCTATGCAGTTTCGTTTGTTAAAGATGCAGAAGTTGTTCATGAATTGAAGAATTATCTGCGAAGCTGTAATGCGGATATACATGTTATTGTAAAAATTGAAAGTGCAGACTCGATCCCAAATTTGCATTCGATTATCACAGCATCTGATGGGGCCATGGTTGCAAGAGGAGATCTAGGTGCAGAACTCCCTGTTGAAGAGGTCCCACTTCTGCAGCATGGGGAAGCTGTTATTGTTGCAACAAATATGCTTGAGAGCATGATTGTTCACCCAACCCCAACCAGAGCTGAGGTATCAGACATTGCCATTGCTGTCCGGGAGGGTGCTGATGCAGTTATGCTGTCGGGAGAAACTGCTAATGGAAAGTATCCGCTGAAAGCCGTGAAAGTTATGCACACTGTTGCTCTGCGGACAGAGGCAACCGTCACAGGTGGCCAAATGCCAGCCAATCTTGGCAAAGCTTTTAAGAATCATATGAGCGAGATGTTTGCTTACCATGCAACCATGATGTCCAATACTCTTGGAACCTCAATCGTCGTGTTCACTAGATCTGGTTTCATGGCTATTCTATTGAGCCATTTTCGTCCTTCTGGGACAATATTTGCTTTTACAAACGATAAGAGGATACAACAGAGACTGGCTCTGTATGAAGGTGTATGTCCCATACACATGGAGTTTGCAGATGATGCTGAAAAAAGCTTCACTAATGCCTTGGCCGTACTGCAGAATCAAGGAATGGTAAAGGCAGGAGAAGAGGTCGCACTCGTTCAGAGTGGCAGACAGCCCATTTGGCGGCTCCAGTCCACTCACAATATTCAAGTGCGCAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

568

Amino Acids

62.24

Weight (kDa)

7.71

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 108 - 419 2.4e-103 Pyruvate kinase, barrel domain
PK_C PF02887 455 - 548 7.6e-18 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52920
fragaria_vesca FvH4_7g30750
malus_domestica MD01G1212800.v1.1 MD07G1282700.v1.1
prunus_persica Prupe.2G305000_v2.0.a1
pyrus_communis pycom01g22390 pycom07g25700
rosa_chinensis RchiOBHm_Chr1g0379701
rosa_laevigata RLG00000026366
rosa_multiflora Rmu_sc0013028.1_g000007
rosa_rugosa Rorug01G0414700
rosa_samantha Rh1AG438100 Rh1BG394600 Rh1CG407600 Rh1DG425000
rosa_wichuraiana Rw1G038180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1697
Acc36I ACCTGC 1 cut(s) 558
AccII CGCG 1 cut(s) 412
AccIII TCCGGA 1 cut(s) 56
AclWI GGATC 4 cut(s) 34, 54, 67, 982
AcoI YGGCCR 3 cut(s) 197, 1306, 1587
AcsI RAATTY 3 cut(s) 584, 848, 994
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 1593
AfiI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 580, 1665
AflII CTTAAG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 5 cut(s) 787, 922, 974, 1070, 1691
AjnI CCWGG 2 cut(s) 66, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 985, 1017
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 9 cut(s) 36, 78, 88, 102, 939, 1100, 1160, 1334, 1572
AluI AGCT 9 cut(s) 36, 78, 88, 102, 939, 1100, 1160, 1334, 1572
Alw21I GWGCWC 3 cut(s) 38, 80, 143
Alw26I GTCTC 3 cut(s) 313, 447, 1501
AlwI GGATC 4 cut(s) 34, 54, 67, 982
AlwNI CAGNNNCTG 2 cut(s) 1019, 1510
Ama87I CYCGRG 2 cut(s) 50, 230
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 6 cut(s) 197, 526, 570, 1026, 1306, 1587
ApaI GGGCCC 1 cut(s) 530
ApeKI GCWGC 2 cut(s) 162, 1087
ApoI RAATTY 3 cut(s) 584, 848, 994
ArsI GACNNNNNNTTYG 2 cut(s) 1644, 1676
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 909
AspLEI GCGC 3 cut(s) 48, 412, 1698
AspS9I GGNCC 7 cut(s) 54, 350, 526, 527, 571, 1026, 1075
AsuC2I CCSGG 3 cut(s) 51, 52, 1190
AsuHPI GGTGA 5 cut(s) 293, 557, 683, 724, 1133
AvaI CYCGRG 2 cut(s) 50, 230
AvaII GGWCC 3 cut(s) 54, 350, 1075
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 1 cut(s) 530
BalI TGGCCA 1 cut(s) 1308
BamHI GGATCC 1 cut(s) 59
BanII GRGCYC 3 cut(s) 38, 80, 530
BauI CACGAG 1 cut(s) 366
Bbv12I GWGCWC 3 cut(s) 38, 80, 143
BbvCI CCTCAGC 2 cut(s) 390, 1161
BbvI GCAGC 2 cut(s) 174, 1099
BccI CCATC 3 cut(s) 683, 749, 1016
BceAI ACGGC 3 cut(s) 184, 1238, 1574
BcgI CGANNNNNNTGC 2 cut(s) 1343, 1377
BciT130I CCWGG 2 cut(s) 68, 576
BciVI GTATCC 2 cut(s) 1251, 1491
BcnI CCSGG 3 cut(s) 51, 52, 1190
BcoDI GTCTC 3 cut(s) 313, 447, 1501
BfaI CTAG 4 cut(s) 774, 1049, 1419, 1705
BfmI CTRYAG 2 cut(s) 1085, 1595
BfrI CTTAAG 1 cut(s) 767
BfuAI ACCTGC 1 cut(s) 558
BfuI GTATCC 2 cut(s) 1251, 1491
BglI GCCNNNNNGGC 1 cut(s) 1663
BglII AGATCT 2 cut(s) 1045, 1421
BisI GCNGC 7 cut(s) 95, 163, 189, 197, 779, 1088, 1667
BlsI GCNGC 7 cut(s) 96, 164, 190, 198, 780, 1089, 1668
Bme1390I CCNGG 5 cut(s) 51, 52, 68, 576, 1190
Bme18I GGWCC 3 cut(s) 54, 350, 1075
BmeT110I CYCGRG 2 cut(s) 50, 230
BmgT120I GGNCC 7 cut(s) 54, 350, 526, 527, 571, 1026, 1075
BmiI GGNNCC 8 cut(s) 55, 61, 528, 573, 1027, 1077, 1399, 1670
BmrFI CCNGG 5 cut(s) 51, 52, 68, 576, 1190
BmsI GCATC 4 cut(s) 889, 1025, 1192, 1549
BoxI GACNNNNGTC 1 cut(s) 212
BplI GAGNNNNNCTC 2 cut(s) 1498, 1530
BpmI CTGGAG 1 cut(s) 1655
Bpu10I CCTNAGC 3 cut(s) 6, 390, 1161
BpuEI CTTGAG 2 cut(s) 74, 1145
BpuMI CCSGG 3 cut(s) 51, 52, 1190
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 1614, 1644
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 580, 1665
Bse1I ACTGG 2 cut(s) 1515, 1672
Bse21I CCTNAGG 1 cut(s) 531
Bse3DI GCAATG 3 cut(s) 501, 1173, 1179
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 2 cut(s) 68, 576
BseGI GGATG 3 cut(s) 177, 405, 420
BseLI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 580, 1665
BseMI GCAATG 3 cut(s) 501, 1173, 1179
BseMII CTCAG 7 cut(s) 20, 171, 381, 522, 747, 816, 1152
BseNI ACTGG 2 cut(s) 1515, 1672
BseRI GAGGAG 3 cut(s) 143, 774, 1056
BseSI GKGCMC 1 cut(s) 530
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 174, 1099
BseYI CCCAGC 1 cut(s) 555
BsgI GTGCAG 2 cut(s) 999, 1074
Bsh1236I CGCG 1 cut(s) 412
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 6 cut(s) 199, 528, 572, 1028, 1308, 1589
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 3 cut(s) 38, 80, 143
BsiHKCI CYCGRG 2 cut(s) 50, 230
BsiSI CCGG 4 cut(s) 51, 57, 148, 1189
BslFI GGGAC 5 cut(s) 84, 409, 1061, 1480, 1518
BslI CCNNNNNNNGG 6 cut(s) 56, 72, 154, 195, 580, 1665
BsmAI GTCTC 3 cut(s) 313, 447, 1501
BsmBI CGTCTC 2 cut(s) 313, 447
BsmFI GGGAC 5 cut(s) 84, 409, 1061, 1480, 1518
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 6 cut(s) 199, 528, 572, 1028, 1308, 1589
BsoBI CYCGRG 2 cut(s) 50, 230
Bsp120I GGGCCC 1 cut(s) 526
Bsp1286I GDGCHC 4 cut(s) 38, 80, 143, 530
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
Bsp19I CCATGG 2 cut(s) 427, 1029
BspANI GGCC 6 cut(s) 199, 528, 572, 1028, 1308, 1589
BspCNI CTCAG 7 cut(s) 19, 172, 382, 523, 746, 817, 1153
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 8 cut(s) 55, 61, 528, 573, 1027, 1077, 1399, 1670
BspMAI CTGCAG 2 cut(s) 1089, 1599
BspMI ACCTGC 1 cut(s) 558
BspPI GGATC 4 cut(s) 34, 54, 67, 982
BspQI GCTCTTC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 767
BsrDI GCAATG 3 cut(s) 501, 1173, 1179
BsrI ACTGG 2 cut(s) 1515, 1672
BssMI GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
BssSI CACGAG 1 cut(s) 366
BssT1I CCWWGG 5 cut(s) 427, 489, 522, 1029, 1584
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 2 cut(s) 68, 576
Bst4CI ACNGT 3 cut(s) 316, 1273, 1297
Bst6I CTCTTC 3 cut(s) 131, 1065, 1620
BstAFI CTTAAG 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 1559
BstC8I GCNNGC 1 cut(s) 1317
BstDEI CTNAG 7 cut(s) 6, 180, 390, 531, 733, 825, 1161
BstDSI CCRYGG 2 cut(s) 427, 1029
BstEII GGTNACC 1 cut(s) 545
BstF5I GGATG 3 cut(s) 177, 405, 420
BstFNI CGCG 1 cut(s) 412
BstHHI GCGC 3 cut(s) 48, 412, 1698
BstKTI GATC 8 cut(s) 42, 62, 186, 378, 436, 990, 1048, 1424
BstMAI GTCTC 3 cut(s) 313, 447, 1501
BstMBI GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 8 cut(s) 23, 94, 253, 812, 1034, 1370, 1559, 1663
BstNI CCWGG 2 cut(s) 68, 576
BstNSI RCATGY 2 cut(s) 679, 959
BstPAI GACNNNNGTC 1 cut(s) 212
BstPI GGTNACC 1 cut(s) 545
BstSCI CCNGG 5 cut(s) 49, 50, 66, 574, 1188
BstSFI CTRYAG 2 cut(s) 1085, 1595
BstSLI GKGCMC 1 cut(s) 530
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 174, 1099
BstX2I RGATCY 3 cut(s) 59, 1045, 1421
BstXI CCANNNNNNTGG 2 cut(s) 118, 1543
BstYI RGATCY 3 cut(s) 59, 1045, 1421
BstZI CGGCCG 1 cut(s) 197
Bsu36I CCTNAGG 1 cut(s) 531
BsuI GTATCC 2 cut(s) 1251, 1491
BsuRI GGCC 6 cut(s) 199, 528, 572, 1028, 1308, 1589
BtgI CCRYGG 2 cut(s) 427, 1029
BtsCI GGATG 3 cut(s) 177, 405, 420
BtsIMutI CAGTG 5 cut(s) 297, 321, 741, 822, 1269
BveI ACCTGC 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 1317
CaiI CAGNNNCTG 2 cut(s) 1019, 1510
CciI TCATGA 1 cut(s) 913
CfoI GCGC 3 cut(s) 48, 412, 1698
Cfr13I GGNCC 7 cut(s) 54, 350, 526, 527, 571, 1026, 1075
Cfr9I CCCGGG 1 cut(s) 50
CseI GACGC 1 cut(s) 429
Csp6I GTAC 1 cut(s) 1592
CviQI GTAC 1 cut(s) 1592
DdeI CTNAG 7 cut(s) 6, 180, 390, 531, 733, 825, 1161
DpnI GATC 8 cut(s) 41, 61, 185, 377, 435, 989, 1047, 1423
DpnII GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
EaeI YGGCCR 3 cut(s) 197, 1306, 1587
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 131, 1065, 1620
EarI CTCTTC 3 cut(s) 131, 1065, 1620
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 2 cut(s) 36, 78
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 5 cut(s) 427, 489, 522, 1029, 1584
Eco24I GRGCYC 3 cut(s) 38, 80, 530
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 3 cut(s) 54, 350, 1075
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 2 cut(s) 36, 78
Eco57I CTGAAG 1 cut(s) 273
Eco81I CCTNAGG 1 cut(s) 531
Eco88I CYCGRG 2 cut(s) 50, 230
Eco91I GGTNACC 1 cut(s) 545
EcoICRI GAGCTC 2 cut(s) 36, 78
EcoO109I RGGNCCY 3 cut(s) 526, 527, 1075
EcoO65I GGTNACC 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 66, 574
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 5 cut(s) 427, 489, 522, 1029, 1584
EcoT38I GRGCYC 3 cut(s) 38, 80, 530
ErhI CCWWGG 5 cut(s) 427, 489, 522, 1029, 1584
Esp3I CGTCTC 2 cut(s) 313, 447
FaqI GGGAC 5 cut(s) 84, 409, 1061, 1480, 1518
FauI CCCGC 1 cut(s) 388
FauNDI CATATG 1 cut(s) 1347
Fnu4HI GCNGC 7 cut(s) 95, 163, 189, 197, 779, 1088, 1667
FokI GGATG 3 cut(s) 184, 412, 427
FriOI GRGCYC 3 cut(s) 38, 80, 530
Fsp4HI GCNGC 7 cut(s) 95, 163, 189, 197, 779, 1088, 1667
FspBI CTAG 4 cut(s) 774, 1049, 1419, 1705
FspI TGCGCA 1 cut(s) 1697
GlaI GCGC 3 cut(s) 47, 411, 1697
GluI GCNGC 7 cut(s) 95, 163, 189, 197, 779, 1088, 1667
GsaI CCCAGC 1 cut(s) 559
GsuI CTGGAG 1 cut(s) 1655
HaeIII GGCC 6 cut(s) 199, 528, 572, 1028, 1308, 1589
HapII CCGG 4 cut(s) 51, 57, 148, 1189
HgaI GACGC 1 cut(s) 429
HhaI GCGC 3 cut(s) 48, 412, 1698
Hin6I GCGC 3 cut(s) 46, 410, 1696
HinP1I GCGC 3 cut(s) 46, 410, 1696
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 1332, 1570
HinfI GANTC 6 cut(s) 28, 268, 731, 983, 1342, 1600
HpaII CCGG 4 cut(s) 51, 57, 148, 1189
HphI GGTGA 5 cut(s) 293, 557, 683, 724, 1133
Hpy166II GTNNAC 6 cut(s) 303, 637, 871, 1141, 1416, 1677
Hpy188I TCNGA 8 cut(s) 33, 82, 181, 450, 603, 1021, 1171, 1644
Hpy188III TCNNGA 6 cut(s) 57, 111, 368, 379, 914, 1218
Hpy8I GTNNAC 6 cut(s) 303, 637, 871, 1141, 1416, 1677
Hpy99I CGWCG 1 cut(s) 1412
HpyAV CCTTC 5 cut(s) 162, 363, 445, 1470, 1517
HpyCH4III ACNGT 3 cut(s) 316, 1273, 1297
HpyCH4IV ACGT 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 94, 253, 812, 1034, 1370, 1559, 1663
HpyF3I CTNAG 7 cut(s) 6, 180, 390, 531, 733, 825, 1161
HpySE526I ACGT 1 cut(s) 322
HspAI GCGC 3 cut(s) 46, 410, 1696
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 75, 83, 1674
Lsp1109I GCAGC 2 cut(s) 174, 1099
LweI GCATC 4 cut(s) 889, 1025, 1192, 1549
MaeI CTAG 4 cut(s) 774, 1049, 1419, 1705
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 5 cut(s) 201, 281, 545, 671, 1297
MalI GATC 8 cut(s) 41, 61, 185, 377, 435, 989, 1047, 1423
MboI GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
MboII GAAGA 6 cut(s) 145, 148, 851, 934, 1082, 1637
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 3 cut(s) 59, 1045, 1421
MhlI GDGCHC 4 cut(s) 38, 80, 143, 530
MlsI TGGCCA 1 cut(s) 1308
MluNI TGGCCA 1 cut(s) 1308
MlyI GAGTC 2 cut(s) 725, 977
MmeI TCCRAC 1 cut(s) 592
Mox20I TGGCCA 1 cut(s) 1308
MroI TCCGGA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 909
MscI TGGCCA 1 cut(s) 1308
MseI TTAA 6 cut(s) 468, 564, 654, 768, 894, 1338
MslI CAYNNNNRTG 3 cut(s) 290, 786, 1541
Msp20I TGGCCA 1 cut(s) 1308
MspA1I CMGCKG 1 cut(s) 1246
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 4 cut(s) 51, 57, 148, 1189
MspR9I CCNGG 5 cut(s) 51, 52, 68, 576, 1190
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 2 cut(s) 68, 576
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 8 cut(s) 23, 94, 253, 812, 1034, 1370, 1559, 1663
NciI CCSGG 3 cut(s) 51, 52, 1190
NcoI CCATGG 2 cut(s) 427, 1029
NdeI CATATG 1 cut(s) 1347
NdeII GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
NlaIV GGNNCC 8 cut(s) 55, 61, 528, 573, 1027, 1077, 1399, 1670
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 4 cut(s) 281, 545, 671, 1297
NsbI TGCGCA 1 cut(s) 1697
NspI RCATGY 2 cut(s) 679, 959
OliI CACNNNNGTG 1 cut(s) 290
PagI TCATGA 1 cut(s) 913
PasI CCCWGGG 2 cut(s) 67, 575
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 1 cut(s) 1000
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 4 cut(s) 28, 268, 1342, 1600
PflFI GACNNNGTC 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 1188
PkrI GCNGC 7 cut(s) 96, 164, 190, 198, 780, 1089, 1668
PleI GAGTC 2 cut(s) 725, 977
PpsI GAGTC 2 cut(s) 725, 977
PpuMI RGGWCCY 1 cut(s) 1075
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 212
PshBI ATTAAT 1 cut(s) 564
Psp124BI GAGCTC 2 cut(s) 38, 80
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 2 cut(s) 66, 574
PspEI GGTNACC 1 cut(s) 545
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 66, 574
PspN4I GGNNCC 8 cut(s) 55, 61, 528, 573, 1027, 1077, 1399, 1670
PspOMI GGGCCC 1 cut(s) 526
PspPI GGNCC 7 cut(s) 54, 350, 526, 527, 571, 1026, 1075
PspPPI RGGWCCY 1 cut(s) 1075
PstI CTGCAG 2 cut(s) 1089, 1599
PstNI CAGNNNCTG 2 cut(s) 1019, 1510
PsuI RGATCY 3 cut(s) 59, 1045, 1421
PsyI GACNNNGTC 1 cut(s) 497
RsaI GTAC 1 cut(s) 1593
RsaNI GTAC 1 cut(s) 1592
RseI CAYNNNNRTG 3 cut(s) 290, 786, 1541
SacI GAGCTC 2 cut(s) 38, 80
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 6 cut(s) 468, 564, 654, 768, 894, 1338
SatI GCNGC 7 cut(s) 95, 163, 189, 197, 779, 1088, 1667
Sau3AI GATC 8 cut(s) 39, 59, 183, 375, 433, 987, 1045, 1421
Sau96I GGNCC 7 cut(s) 54, 350, 526, 527, 571, 1026, 1075
SchI GAGTC 2 cut(s) 725, 977
ScrFI CCNGG 5 cut(s) 51, 52, 68, 576, 1190
SduI GDGCHC 4 cut(s) 38, 80, 143, 530
SfaNI GCATC 4 cut(s) 889, 1025, 1192, 1549
SfcI CTRYAG 2 cut(s) 1085, 1595
SinI GGWCC 3 cut(s) 54, 350, 1075
SmaI CCCGGG 1 cut(s) 52
SmiMI CAYNNNNRTG 3 cut(s) 290, 786, 1541
SmlI CTYRAG 3 cut(s) 89, 767, 1124
SmoI CTYRAG 3 cut(s) 89, 767, 1124
SspI AATATT 2 cut(s) 1473, 1687
SspMI CTAG 4 cut(s) 774, 1049, 1419, 1705
SstI GAGCTC 2 cut(s) 38, 80
StyD4I CCNGG 5 cut(s) 49, 50, 66, 574, 1188
StyI CCWWGG 5 cut(s) 427, 489, 522, 1029, 1584
TaaI ACNGT 3 cut(s) 316, 1273, 1297
TaiI ACGT 1 cut(s) 325
TaqI TCGA 5 cut(s) 38, 459, 753, 986, 1004
TaqII GACCGA 1 cut(s) 224
TauI GCSGC 5 cut(s) 97, 191, 199, 781, 1669
TfiI GAWTC 4 cut(s) 28, 268, 1342, 1600
Tru1I TTAA 6 cut(s) 468, 564, 654, 768, 894, 1338
Tru9I TTAA 6 cut(s) 468, 564, 654, 768, 894, 1338
TscAI CASTG 5 cut(s) 297, 321, 741, 829, 1276
TseFI GTSAC 4 cut(s) 281, 545, 671, 1297
TseI GCWGC 2 cut(s) 162, 1087
Tsp45I GTSAC 4 cut(s) 281, 545, 671, 1297
TspDTI ATGAA 6 cut(s) 416, 431, 902, 930, 1420, 1536
TspGWI ACGGA 1 cut(s) 88
TspMI CCCGGG 1 cut(s) 50
TspRI CASTG 5 cut(s) 297, 321, 741, 829, 1276
Tth111I GACNNNGTC 1 cut(s) 497
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 3 cut(s) 54, 350, 1075
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 3 cut(s) 584, 848, 994
XceI RCATGY 2 cut(s) 679, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmaI CCCGGG 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 4 cut(s) 774, 1049, 1419, 1705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.