MD07G1282700.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015479
Physical Location & Seq
Forward (+)
34578415 .. 34582623
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1282700.v1.1.491

Sequence Viewer

Length: 2136 bp
ATGCGTAGGCAGAAGTGTTGGTCCGAAACAGGCCCAAAACCGTCTCGTGACGTGACCCACCCGTGCCCCTTTCGAGACAAGCTCAGCTTTGAACGTAAGAAGTTGAACAACATTTTACTTTTGTTCGATACAACCAAAGCCCTCCCGTCCCTCCTAAACACTCTTTATCCATCTGTCGCCTCAAAACCTCCGCCAAATTCAGCGGCGACTTCAACACGATTTAACCACTCCCTACCTACATACATACGTTCTTTTAAATCCCTCCGCCACACACTTCCCCCTCACCTTCCTCCTCTCCCTCAGAACCTCCGCATTCCCCTTCCAAAAACCCGAATCTCCCTCCCTCTTCCTCTCTCTCTCTCTTTCCTTCCAAACAAAATGGCGCACGTGGTGGCTATGCGGGCGATTCATAGCTCGATCCTCTCTCCCAGCTCTGGATCTGCACGCGGCCGCGCCGCCGACAAGCTGAAGCCGCCGACGAGCTTTGCGTACAAAGTTATGGCTTTTGAGGAGAAGCAGAAGGGATGGAGGTGGAGAGTGTTTGGGAATAGGAGGTCTGAGATCACCGCCAAGCTTCCCCTTCAGACGGAGGTCGTTCCCGTCTCGCCCGAAGACTCGCCCAAGATAGAGGATCAGCTGAGGGCAATTCAGCAACTTGACGACACAGCAGTGGGTATGTGGTCAAGGCCTGTGGTCAAACGTAAGACAAAGATTGTTTGCACAATTGGTCCTTCCACCAACACGCGCGAAATGATCTGGAAGTTGGCAGAGGCCGGAATGAATGTTGCTCGTATGAATATGTCGCATGGAGATCACGCGTCTCATCAAAAAGTTATCGACTTGGTTAAAGAATATAATGAACAACATGAAGACAATGCCATTGCGATCATGCTTGATACCAAGGCAAGGCTCCCCCTATTGTTTCTTGGTCCTGAGGTTAGGAGCGGTGACCTGCCACAGCCAATCAATTTAGAAAGTGGGCAGGAATTTACCTTTACAATCAAAAGAGGGGTTGGCACAGCAGATTGTGTTAGCGTGAACTATGATGATTTTGTTAACGATGTAGCACCGGGAGACATGCTTTTGGTTGATGGTGGTATGATGTCATTTCTGGTGAAGTCCAAGACAGAAGAATCAGTGAAATGTGAAGTTGTTGATGGAGGAGAGCTTAAGTCTAGGCGACATTTGAATGTGCGAGGGAAAAGTGCAACATTGCCTTCTATCACTGAGAAAGATTGGGAAGATATTAAATTTGGAGTGGACAATAAAGTTGACTTCTACGCTGTTTCATTTGTCAAAGATGCGCAAGTTGTTCATGAATTGAAGAATTATCTGCAAAGTAATAATGCAGATATACACGTTATTGTTAAAATAGAAAGTGCAGACTCTATCCCAAATTTGCATTCGATTATCACAGCATCCGATGGGGCAATGGTTGCAAGAGGAGATCTTGGTGCAGAGCTCCCTGTTGAGGAAGTTCCGCTTTTGCAGGAAGAGATAATTAGGATATGCCGTAGCATGGGAAAGGCTGTTATTGTGGCAACAAATATGCTGGAAAGCATGATTGTTCATCCAACACCAACCAGAGCTGAGGTATCCGACATTGCCATTGCTGTTAGAGAGGGTTCTGATGGAATTATGCTTTCTGGAGAGACTGCTCATGGAAAGTTTCCTTTGAAGGCAGTGAAAGTTATGCACACTGTCGCACTTCGGACTGAGGCGACTATCTTGGGTGGCGCAGTGCCTGCCAATCTTGGTAAAGCTTTCAAGAACCATATGAGTGAGATGTTTGCTTACCATGCAACCATGATGTCCAATACTCTTGGTACCTCAATCGTTGTCTTTACCAGATCTGGTTTCATGGCTATTCTATTGAGCCATTACCGACCTACCGGGACCATATTTGCCTTTACGAATGATAAGAGCATACAACGGAGGTTGGCTTTGTATCAAGGCGTATGTCCCATATACATGGAGTTCACAGAAGATTCTGAAACAAGCTTTAACAATGCCTTGACTGTGCTGAAGAAGCAAGGATTGGTAAAGGCAGGAGAAGAGGTCGCACTCGTTCAAAGTGGCAGGCAACCCATTTGGCGGCTCCAATCCACTCACAACATTCAAGTGCGCAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

712

Amino Acids

78.85

Weight (kDa)

9.28

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 234 - 562 1.3e-107 Pyruvate kinase, barrel domain
PK_C PF02887 598 - 691 3.3e-20 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52920
fragaria_vesca FvH4_7g30750
malus_domestica MD01G1212800.v1.1 MD07G1282700.v1.1
prunus_persica Prupe.2G305000_v2.0.a1
pyrus_communis pycom01g22390 pycom07g25700
rosa_chinensis RchiOBHm_Chr1g0379701
rosa_laevigata RLG00000026366
rosa_multiflora Rmu_sc0013028.1_g000007
rosa_rugosa Rorug01G0414700
rosa_samantha Rh1AG438100 Rh1BG394600 Rh1CG407600 Rh1DG425000
rosa_wichuraiana Rw1G038180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1305, 2126
Acc36I ACCTGC 1 cut(s) 960
Acc65I GGTACC 1 cut(s) 1826
AccB1I GGYRCC 1 cut(s) 1826
AccBSI CCGCTC 1 cut(s) 945
AccII CGCG 5 cut(s) 447, 453, 745, 747, 818
AclWI GGATC 3 cut(s) 412, 445, 639
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 5 cut(s) 196, 986, 1250, 1396, 2130
AcuI CTGAAG 3 cut(s) 488, 566, 2045
AcvI CACGTG 1 cut(s) 388
AdeI CACNNNGTG 1 cut(s) 391
AfaI GTAC 2 cut(s) 491, 1828
AfiI CCNNNNNNNGG 6 cut(s) 434, 586, 906, 1471, 1519, 2094
AflII CTTAAG 1 cut(s) 1169
AflIII ACRYGT 2 cut(s) 816, 1357
AgsI TTSAA 9 cut(s) 92, 106, 213, 1189, 1324, 1678, 1768, 2072, 2120
AjiI CACGTC 1 cut(s) 52
AjuI GAANNNNNNNTTGG 4 cut(s) 1387, 1419, 2021, 2053
AleI CACNNNNGTG 1 cut(s) 668
Alw21I GWGCWC 1 cut(s) 1464
Alw26I GTCTC 6 cut(s) 48, 69, 607, 825, 1068, 1646
AlwI GGATC 3 cut(s) 412, 445, 639
AlwNI CAGNNNCTG 1 cut(s) 1745
AoxI GGCC 4 cut(s) 31, 448, 686, 771
ApoI RAATTY 5 cut(s) 196, 986, 1250, 1396, 2130
Asp718I GGTACC 1 cut(s) 1826
AspLEI GCGC 6 cut(s) 385, 455, 747, 1306, 1739, 2127
AspS9I GGNCC 5 cut(s) 21, 32, 728, 929, 1896
AsuC2I CCSGG 2 cut(s) 1071, 1894
AsuHPI GGTGA 4 cut(s) 275, 556, 959, 1126
AvaII GGWCC 4 cut(s) 21, 728, 929, 1896
AxyI CCTNAGG 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 68
BanI GGYRCC 1 cut(s) 1826
BanII GRGCYC 1 cut(s) 1464
BauI CACGAG 1 cut(s) 45
BbrPI CACGTG 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 618, 876
Bbv12I GWGCWC 1 cut(s) 1464
BbvCI CCTCAGC 2 cut(s) 638, 1590
BccI CCATC 6 cut(s) 178, 519, 1085, 1151, 1418, 1625
BceAI ACGGC 1 cut(s) 1497
BciVI GTATCC 1 cut(s) 1606
BcnI CCSGG 2 cut(s) 1071, 1894
BcoDI GTCTC 6 cut(s) 48, 69, 607, 825, 1068, 1646
BfaI CTAG 1 cut(s) 1176
BfrI CTTAAG 1 cut(s) 1169
BfuAI ACCTGC 1 cut(s) 960
BfuI GTATCC 1 cut(s) 1606
BglII AGATCT 2 cut(s) 1447, 1850
BisI GCNGC 6 cut(s) 204, 448, 451, 456, 473, 2096
BlpI GCTNAGC 1 cut(s) 83
BlsI GCNGC 6 cut(s) 205, 449, 452, 457, 474, 2097
Bme1390I CCNGG 2 cut(s) 1071, 1894
Bme18I GGWCC 4 cut(s) 21, 728, 929, 1896
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 5 cut(s) 21, 32, 728, 929, 1896
BmiI GGNNCC 4 cut(s) 911, 1828, 1897, 2099
BmrFI CCNGG 2 cut(s) 1071, 1894
BmsI GCATC 2 cut(s) 1291, 1427
BoxI GACNNNNGTC 1 cut(s) 590
BpiI GAAGAC 2 cut(s) 618, 876
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 1668
Bpu10I CCTNAGC 2 cut(s) 638, 1590
Bpu1102I GCTNAGC 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 1071, 1894
BsaAI YACGTR 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 900
BsaXI ACNNNNNCTCC 2 cut(s) 2043, 2073
Bsc4I CCNNNNNNNGG 6 cut(s) 434, 586, 906, 1471, 1519, 2094
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 5 cut(s) 879, 1211, 1437, 1602, 1608
BseDI CCNNGG 1 cut(s) 900
BseGI GGATG 3 cut(s) 530, 1418, 1570
BseLI CCNNNNNNNGG 6 cut(s) 434, 586, 906, 1471, 1519, 2094
BseMI GCAATG 5 cut(s) 879, 1211, 1437, 1602, 1608
BseMII CTCAG 8 cut(s) 97, 314, 549, 629, 924, 1218, 1581, 1707
BseRI GAGGAG 4 cut(s) 282, 524, 1176, 1458
BseSI GKGCMC 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 448
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 3 cut(s) 426, 1401, 1476
Bsh1236I CGCG 5 cut(s) 447, 453, 745, 747, 818
Bsh1285I CGRYCG 1 cut(s) 451
BshFI GGCC 4 cut(s) 33, 450, 688, 773
BshNI GGYRCC 1 cut(s) 1826
BsiEI CGRYCG 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 1464
BsiSI CCGG 3 cut(s) 774, 1070, 1893
BslFI GGGAC 3 cut(s) 133, 1909, 1947
BslI CCNNNNNNNGG 6 cut(s) 434, 586, 906, 1471, 1519, 2094
BsmAI GTCTC 6 cut(s) 48, 69, 607, 825, 1068, 1646
BsmBI CGTCTC 3 cut(s) 48, 607, 825
BsmFI GGGAC 3 cut(s) 133, 1909, 1947
BsmI GAATGC 2 cut(s) 312, 1402
BsnI GGCC 4 cut(s) 33, 450, 688, 773
Bsp1286I GDGCHC 2 cut(s) 68, 1464
Bsp143I GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
Bsp1720I GCTNAGC 1 cut(s) 83
BspANI GGCC 4 cut(s) 33, 450, 688, 773
BspCNI CTCAG 8 cut(s) 96, 313, 550, 630, 925, 1219, 1582, 1708
BspFNI CGCG 5 cut(s) 447, 453, 745, 747, 818
BspHI TCATGA 1 cut(s) 1315
BspLI GGNNCC 4 cut(s) 911, 1828, 1897, 2099
BspMI ACCTGC 1 cut(s) 960
BspPI GGATC 3 cut(s) 412, 445, 639
BspT107I GGYRCC 1 cut(s) 1826
BspTI CTTAAG 1 cut(s) 1169
BsrBI CCGCTC 1 cut(s) 945
BsrDI GCAATG 5 cut(s) 879, 1211, 1437, 1602, 1608
BssECI CCNNGG 1 cut(s) 900
BssMI GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 900
Bst2BI CACGAG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 42, 1702, 2020
Bst6I CTCTTC 3 cut(s) 351, 1488, 2049
BstAFI CTTAAG 1 cut(s) 1169
BstAPI GCANNNNNTGC 2 cut(s) 1436, 1745
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 4 cut(s) 402, 445, 1746, 2081
BstDEI CTNAG 8 cut(s) 83, 300, 558, 638, 933, 1227, 1590, 1716
BstEII GGTNACC 1 cut(s) 947
BstF5I GGATG 3 cut(s) 530, 1418, 1570
BstFNI CGCG 5 cut(s) 447, 453, 745, 747, 818
BstHHI GCGC 6 cut(s) 385, 455, 747, 1306, 1739, 2127
BstKTI GATC 9 cut(s) 420, 440, 564, 634, 756, 814, 888, 1450, 1853
BstMAI GTCTC 6 cut(s) 48, 69, 607, 825, 1068, 1646
BstMBI GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
BstMCI CGRYCG 1 cut(s) 451
BstMWI GCNNNNNNNGC 6 cut(s) 401, 472, 1436, 1745, 1799, 2029
BstNSI RCATGY 1 cut(s) 1081
BstPAI GACNNNNGTC 1 cut(s) 590
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 2 cut(s) 1069, 1892
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 5 cut(s) 447, 453, 745, 747, 818
BstV2I GAAGAC 2 cut(s) 618, 876
BstX2I RGATCY 3 cut(s) 437, 1447, 1850
BstXI CCANNNNNNTGG 1 cut(s) 1972
BstYI RGATCY 3 cut(s) 437, 1447, 1850
BstZI CGGCCG 1 cut(s) 448
Bsu36I CCTNAGG 1 cut(s) 933
BsuI GTATCC 1 cut(s) 1606
BsuRI GGCC 4 cut(s) 33, 450, 688, 773
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 3 cut(s) 530, 1418, 1570
BtsI GCAGTG 3 cut(s) 675, 1689, 1746
BtsIMutI CAGTG 6 cut(s) 675, 1143, 1224, 1689, 1698, 1746
BveI ACCTGC 1 cut(s) 960
Cac8I GCNNGC 4 cut(s) 402, 445, 1746, 2081
CaiI CAGNNNCTG 1 cut(s) 1745
CciI TCATGA 1 cut(s) 1315
CciNI GCGGCCGC 1 cut(s) 448
CfoI GCGC 6 cut(s) 385, 455, 747, 1306, 1739, 2127
Cfr13I GGNCC 5 cut(s) 21, 32, 728, 929, 1896
CseI GACGC 1 cut(s) 807
Csp6I GTAC 2 cut(s) 490, 1827
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviQI GTAC 2 cut(s) 490, 1827
DdeI CTNAG 8 cut(s) 83, 300, 558, 638, 933, 1227, 1590, 1716
DpnI GATC 9 cut(s) 419, 439, 563, 633, 755, 813, 887, 1449, 1852
DpnII GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
DraI TTTAAA 1 cut(s) 256
DraIII CACNNNGTG 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 448
EagI CGGCCG 1 cut(s) 448
Eam1104I CTCTTC 3 cut(s) 351, 1488, 2049
EarI CTCTTC 3 cut(s) 351, 1488, 2049
EciI GGCGGA 2 cut(s) 180, 254
Ecl136II GAGCTC 1 cut(s) 1462
EclXI CGGCCG 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 900
Eco147I AGGCCT 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 1464
Eco47I GGWCC 4 cut(s) 21, 728, 929, 1896
Eco52I CGGCCG 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 1462
Eco57I CTGAAG 3 cut(s) 488, 566, 2045
Eco72I CACGTG 1 cut(s) 388
Eco81I CCTNAGG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 947
EcoICRI GAGCTC 1 cut(s) 1462
EcoO65I GGTNACC 1 cut(s) 947
EcoT14I CCWWGG 1 cut(s) 900
EcoT38I GRGCYC 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 900
Esp3I CGTCTC 3 cut(s) 48, 607, 825
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 1467, 1499
FaqI GGGAC 3 cut(s) 133, 1909, 1947
FauI CCCGC 1 cut(s) 393
FauNDI CATATG 1 cut(s) 1776
Fnu4HI GCNGC 6 cut(s) 204, 448, 451, 456, 473, 2096
FokI GGATG 3 cut(s) 537, 1405, 1557
FriOI GRGCYC 1 cut(s) 1464
Fsp4HI GCNGC 6 cut(s) 204, 448, 451, 456, 473, 2096
FspBI CTAG 1 cut(s) 1176
FspI TGCGCA 2 cut(s) 1305, 2126
GlaI GCGC 6 cut(s) 384, 454, 746, 1305, 1738, 2126
GluI GCNGC 6 cut(s) 204, 448, 451, 456, 473, 2096
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 1668
HaeIII GGCC 4 cut(s) 33, 450, 688, 773
HapII CCGG 3 cut(s) 774, 1070, 1893
HgaI GACGC 1 cut(s) 807
HhaI GCGC 6 cut(s) 385, 455, 747, 1306, 1739, 2127
Hin6I GCGC 6 cut(s) 383, 453, 745, 1304, 1737, 2125
HinP1I GCGC 6 cut(s) 383, 453, 745, 1304, 1737, 2125
HincII GTYRAC 2 cut(s) 1057, 1273
HindII GTYRAC 2 cut(s) 1057, 1273
HindIII AAGCTT 3 cut(s) 572, 1761, 1999
HinfI GANTC 6 cut(s) 333, 406, 614, 1133, 1385, 1988
HpaI GTTAAC 1 cut(s) 1057
HpaII CCGG 3 cut(s) 774, 1070, 1893
HphI GGTGA 4 cut(s) 275, 556, 959, 1126
Hpy166II GTNNAC 5 cut(s) 1039, 1057, 1261, 1273, 1980
Hpy188I TCNGA 9 cut(s) 25, 303, 559, 585, 1423, 1600, 1630, 1713, 1993
Hpy188III TCNNGA 8 cut(s) 47, 74, 435, 757, 932, 1316, 1647, 1768
Hpy8I GTNNAC 5 cut(s) 1039, 1057, 1261, 1273, 1980
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 8 cut(s) 296, 329, 377, 514, 590, 741, 1227, 1672
HpyCH4III ACNGT 3 cut(s) 42, 1702, 2020
HpyCH4IV ACGT 6 cut(s) 51, 94, 247, 387, 700, 1359
HpyF10VI GCNNNNNNNGC 6 cut(s) 401, 472, 1436, 1745, 1799, 2029
HpyF3I CTNAG 8 cut(s) 83, 300, 558, 638, 933, 1227, 1590, 1716
HpySE526I ACGT 6 cut(s) 51, 94, 247, 387, 700, 1359
HspAI GCGC 6 cut(s) 383, 453, 745, 1304, 1737, 2125
KpnI GGTACC 1 cut(s) 1830
KspAI GTTAAC 1 cut(s) 1057
Kzo9I GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
LmnI GCTCC 4 cut(s) 915, 942, 1467, 2103
LweI GCATC 2 cut(s) 1291, 1427
MaeI CTAG 1 cut(s) 1176
MaeII ACGT 6 cut(s) 51, 94, 247, 387, 700, 1359
MaeIII GTNAC 3 cut(s) 47, 52, 947
MalI GATC 9 cut(s) 419, 439, 563, 633, 755, 813, 887, 1449, 1852
MbiI CCGCTC 1 cut(s) 945
MboI GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 3 cut(s) 437, 1447, 1850
MhlI GDGCHC 2 cut(s) 68, 1464
MluI ACGCGT 1 cut(s) 816
MlyI GAGTC 2 cut(s) 608, 1379
MmeI TCCRAC 2 cut(s) 1598, 1623
MseI TTAA 8 cut(s) 222, 255, 846, 1056, 1170, 1248, 1368, 2004
MslI CAYNNNNRTG 5 cut(s) 668, 1188, 1779, 1970, 2120
MspA1I CMGCKG 2 cut(s) 203, 637
MspCI CTTAAG 1 cut(s) 1169
MspI CCGG 3 cut(s) 774, 1070, 1893
MspR9I CCNGG 2 cut(s) 1071, 1894
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 2 cut(s) 312, 1402
MvnI CGCG 5 cut(s) 447, 453, 745, 747, 818
MwoI GCNNNNNNNGC 6 cut(s) 401, 472, 1436, 1745, 1799, 2029
NciI CCSGG 2 cut(s) 1071, 1894
NdeI CATATG 1 cut(s) 1776
NdeII GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
NlaIV GGNNCC 4 cut(s) 911, 1828, 1897, 2099
NmuCI GTSAC 3 cut(s) 47, 52, 947
NotI GCGGCCGC 1 cut(s) 448
NsbI TGCGCA 2 cut(s) 1305, 2126
NspI RCATGY 1 cut(s) 1081
OliI CACNNNNGTG 1 cut(s) 668
PagI TCATGA 1 cut(s) 1315
PceI AGGCCT 1 cut(s) 688
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PctI GAATGC 2 cut(s) 312, 1402
PfeI GAWTC 4 cut(s) 333, 406, 1133, 1988
PkrI GCNGC 6 cut(s) 205, 449, 452, 457, 474, 2097
PleI GAGTC 2 cut(s) 608, 1379
PmaCI CACGTG 1 cut(s) 388
PmlI CACGTG 1 cut(s) 388
PpsI GAGTC 2 cut(s) 608, 1379
Ppu21I YACGTR 1 cut(s) 388
PshAI GACNNNNGTC 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 1464
PspCI CACGTG 1 cut(s) 388
PspEI GGTNACC 1 cut(s) 947
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 911, 1828, 1897, 2099
PspPI GGNCC 5 cut(s) 21, 32, 728, 929, 1896
PstNI CAGNNNCTG 1 cut(s) 1745
PsuI RGATCY 3 cut(s) 437, 1447, 1850
PvuII CAGCTG 1 cut(s) 637
RsaI GTAC 2 cut(s) 491, 1828
RsaNI GTAC 2 cut(s) 490, 1827
RseI CAYNNNNRTG 5 cut(s) 668, 1188, 1779, 1970, 2120
SacI GAGCTC 1 cut(s) 1464
SaqAI TTAA 8 cut(s) 222, 255, 846, 1056, 1170, 1248, 1368, 2004
SatI GCNGC 6 cut(s) 204, 448, 451, 456, 473, 2096
Sau3AI GATC 9 cut(s) 417, 437, 561, 631, 753, 811, 885, 1447, 1850
Sau96I GGNCC 5 cut(s) 21, 32, 728, 929, 1896
SchI GAGTC 2 cut(s) 608, 1379
ScrFI CCNGG 2 cut(s) 1071, 1894
SduI GDGCHC 2 cut(s) 68, 1464
SfaNI GCATC 2 cut(s) 1291, 1427
SinI GGWCC 4 cut(s) 21, 728, 929, 1896
SmiMI CAYNNNNRTG 5 cut(s) 668, 1188, 1779, 1970, 2120
SmlI CTYRAG 1 cut(s) 1169
SmoI CTYRAG 1 cut(s) 1169
SseBI AGGCCT 1 cut(s) 688
SspMI CTAG 1 cut(s) 1176
SstI GAGCTC 1 cut(s) 1464
StuI AGGCCT 1 cut(s) 688
StyD4I CCNGG 2 cut(s) 1069, 1892
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 3 cut(s) 42, 1702, 2020
TaiI ACGT 6 cut(s) 54, 97, 250, 390, 703, 1362
TaqI TCGA 5 cut(s) 73, 126, 416, 837, 1406
TauI GCSGC 6 cut(s) 206, 450, 453, 458, 475, 2098
TfiI GAWTC 4 cut(s) 333, 406, 1133, 1988
Tru1I TTAA 8 cut(s) 222, 255, 846, 1056, 1170, 1248, 1368, 2004
Tru9I TTAA 8 cut(s) 222, 255, 846, 1056, 1170, 1248, 1368, 2004
TscAI CASTG 6 cut(s) 675, 1143, 1231, 1689, 1705, 1746
TseFI GTSAC 3 cut(s) 47, 52, 947
Tsp45I GTSAC 3 cut(s) 47, 52, 947
TspGWI ACGGA 2 cut(s) 602, 1948
TspRI CASTG 6 cut(s) 675, 1143, 1231, 1689, 1705, 1746
Vha464I CTTAAG 1 cut(s) 1169
VpaK11BI GGWCC 4 cut(s) 21, 728, 929, 1896
XapI RAATTY 5 cut(s) 196, 986, 1250, 1396, 2130
XceI RCATGY 1 cut(s) 1081
XspI CTAG 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.