MD01G1215200.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015416
Physical Location & Seq
Forward (+)
30847251 .. 30849505
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1215200.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGCCATGGACGATCGCGACCGTTCATCTGATTTTCTCGAAGAACATGAGCAGTACTACCACGGCGATACCGATTGTAACTTAGGCACTCTGGGAGACGATCTTTTTAGCATGTTAGAGAGCTTAGATGATCATGGGTTTGGAGGAGGAGGAGGCGGGGGAATTGTGGACTTTCCTCAGCCGTCAGCTACAGCTCCATTGGAATATGAACAACTACTTGCTGTTTTTAGCGCATCACCAAAAGACTTCTCCTCCTCAGCTGATCAGACCGAGCTTGATGAAACTAACTCTGCCCCCAAAACCAAGAGACTCAAGTTCTCATCAGCTGCCACCCCCACCGCCACTGCCACCACAACAACCATGAATCCTTCAGATCAGGATGGAGGGCGGATGTCTCATACAACAGTGGAGCGCAACCGTAGAAAGCAAATGAACGACCACTTGAGTGTCTTGCGCTCTCTTATGCCTTGCTTTTATGTTAAACGAGGAGATCAAGCTTCAATTATTGGAGGGGTGGTTGATTACATCAATGAGCTGCAGCAAGTTCTTCAATCACTTGAAGCCAAAAAGCAACGAAAAGCATACAGCTGTGAAGTGTTAAGCAGCCCTAGGCTGCAGCAAGTTTCCAGTCCAAGGCCACCTGGTCCTTCACCATCAGTCCTCAGCCCTAGAAAGCCACCTCTCAGCCCTAGGCTCGGCGGCAGCAGCTTACCACCCATCAGCCCAAGAACTCCACAACCCACAAGCCCTTATAAGCCAACGGCAGCCAGGACAATATTGCAACAACCACAAATATTACCTGCAGCTCCTAATATTTTTGGTAATTATCTGATTTCTAGTCCAACAATGGTTGCTAGCTCATCACTTGATCCATCCCCTACTTCTTCAAGTGCTACTTCCTCCATTAATAATAGTATCGACAATCTCAACGAGCTTTTCGCCAACTCCAAGTCCGCCATTGCGGATGTGGAGGTGAAATTTTCGGGTCCAAATGTTCTTTTGAAAACAGTGTCTCCTCGGATACCTGGACAGGCTCTAAAAATCATTTCCACTCTTGAAGACCTTTCGCTAGAAATTCTCCATGTCAGTATCACCACCGCTGACGAAACCATGCTTAATTCCTTCACCATCAAGATTGGAATTGAATGCCAACTTAGTGCCGAGGAACTCGTTCACCAAATCCAGCAAACATTCTGCTAA

Protein Analysis

400

Amino Acids

43.06

Weight (kDa)

5.17

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 130 - 180 2.4e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53210 AT5G53210
fragaria_vesca FvH4_7g31020
malus_domestica MD01G1215200.v1.1 MD07G1285100.v1.1
prunus_persica Prupe.2G307300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0380101
rosa_laevigata RLG00000026332
rosa_multiflora Rmu_sc0001232.1_g000008
rosa_roxburghii Rroxscaffold_4G00279700
rosa_rugosa Rorug01G0417400
rosa_samantha Rh1AG442600 Rh1BG397600 Rh1CG411000 Rh1DG428400
rosa_wichuraiana Rw1G038430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
Acc36I ACCTGC 1 cut(s) 808
AccII CGCG 1 cut(s) 18
AciI CCGC 7 cut(s) 156, 339, 388, 699, 954, 962, 1098
AclWI GGATC 1 cut(s) 863
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 977, 1074
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 633, 695
AgsI TTSAA 7 cut(s) 501, 551, 560, 888, 1003, 1058, 1145
AjnI CCWGG 3 cut(s) 640, 767, 1024
AleI CACNNNNGTG 1 cut(s) 444
Alw26I GTCTC 4 cut(s) 90, 301, 399, 1017
AlwI GGATC 1 cut(s) 863
AoxI GGCC 2 cut(s) 3, 635
ApoI RAATTY 2 cut(s) 977, 1074
AseI ATTAAT 1 cut(s) 906
Asp700I GAANNNNTTC 1 cut(s) 1170
AspA2I CCTAGG 2 cut(s) 608, 689
AspLEI GCGC 3 cut(s) 233, 414, 456
AspS9I GGNCC 2 cut(s) 644, 986
AsuHPI GGTGA 6 cut(s) 228, 642, 985, 1084, 1117, 1166
AsuNHI GCTAGC 1 cut(s) 854
AvaII GGWCC 2 cut(s) 644, 986
AvrII CCTAGG 2 cut(s) 608, 689
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 1065
BbvCI CCTCAGC 3 cut(s) 177, 256, 662
BccI CCATC 5 cut(s) 374, 661, 725, 880, 1136
BceAI ACGGC 3 cut(s) 79, 166, 777
BciT130I CCWGG 3 cut(s) 642, 769, 1026
BciVI GTATCC 1 cut(s) 1014
BclI TGATCA 2 cut(s) 130, 262
BcoDI GTCTC 4 cut(s) 90, 301, 399, 1017
BfaI CTAG 6 cut(s) 609, 669, 690, 837, 855, 1070
BfmI CTRYAG 4 cut(s) 189, 536, 614, 801
BfuAI ACCTGC 1 cut(s) 808
BfuI GTATCC 1 cut(s) 1014
BlnI CCTAGG 2 cut(s) 608, 689
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 3 cut(s) 642, 769, 1026
Bme18I GGWCC 2 cut(s) 644, 986
BmgT120I GGNCC 2 cut(s) 644, 986
BmiI GGNNCC 1 cut(s) 987
BmrFI CCNGG 3 cut(s) 642, 769, 1026
BmsI GCATC 1 cut(s) 242
BmtI GCTAGC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 1065
Bpu10I CCTNAGC 3 cut(s) 177, 256, 662
BpuEI CTTGAG 2 cut(s) 296, 463
BsaJI CCNNGG 7 cut(s) 6, 61, 608, 632, 689, 1016, 1161
BsaXI ACNNNNNCTCC 6 cut(s) 236, 266, 501, 531, 997, 1027
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 695
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 957
BseBI CCWGG 3 cut(s) 642, 769, 1026
BseDI CCNNGG 7 cut(s) 6, 61, 608, 632, 689, 1016, 1161
BseGI GGATG 4 cut(s) 385, 396, 872, 970
BseLI CCNNNNNNNGG 2 cut(s) 633, 695
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 4 cut(s) 191, 270, 676, 697
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 7 cut(s) 159, 162, 165, 241, 244, 501, 1005
Bsh1236I CGCG 1 cut(s) 18
Bsh1285I CGRYCG 2 cut(s) 16, 22
BshFI GGCC 2 cut(s) 5, 637
BsiEI CGRYCG 2 cut(s) 16, 22
BslI CCNNNNNNNGG 2 cut(s) 633, 695
BsmAI GTCTC 4 cut(s) 90, 301, 399, 1017
BsmBI CGTCTC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 2 cut(s) 5, 637
Bsp143I GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
Bsp19I CCATGG 1 cut(s) 6
Bsp68I TCGCGA 1 cut(s) 18
BspACI CCGC 7 cut(s) 156, 339, 388, 699, 954, 962, 1098
BspANI GGCC 2 cut(s) 5, 637
BspCNI CTCAG 4 cut(s) 190, 269, 675, 696
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 987
BspMAI CTGCAG 3 cut(s) 540, 618, 805
BspMI ACCTGC 1 cut(s) 808
BspOI GCTAGC 1 cut(s) 858
BspPI GGATC 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 7 cut(s) 6, 61, 608, 632, 689, 1016, 1161
BssMI GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
BssT1I CCWWGG 4 cut(s) 6, 608, 632, 689
Bst2UI CCWGG 3 cut(s) 642, 769, 1026
Bst4CI ACNGT 4 cut(s) 23, 406, 419, 1009
BstC8I GCNNGC 1 cut(s) 856
BstDEI CTNAG 7 cut(s) 82, 124, 177, 256, 662, 683, 1154
BstDSI CCRYGG 2 cut(s) 6, 61
BstF5I GGATG 4 cut(s) 385, 396, 872, 970
BstFNI CGCG 1 cut(s) 18
BstHHI GCGC 3 cut(s) 233, 414, 456
BstKTI GATC 7 cut(s) 16, 103, 133, 265, 376, 493, 871
BstMAI GTCTC 4 cut(s) 90, 301, 399, 1017
BstMBI GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
BstMCI CGRYCG 2 cut(s) 16, 22
BstMWI GCNNNNNNNGC 1 cut(s) 705
BstNI CCWGG 3 cut(s) 642, 769, 1026
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 640, 767, 1024
BstSFI CTRYAG 4 cut(s) 189, 536, 614, 801
BstUI CGCG 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 1065
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 5, 637
BtgI CCRYGG 2 cut(s) 6, 61
BtsCI GGATG 4 cut(s) 385, 396, 872, 970
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 342, 411, 1014
BtuMI TCGCGA 1 cut(s) 18
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 1 cut(s) 856
CfoI GCGC 3 cut(s) 233, 414, 456
Cfr13I GGNCC 2 cut(s) 644, 986
CsiI ACCWGGT 1 cut(s) 640
Csp6I GTAC 1 cut(s) 55
CviAII CATG 7 cut(s) 7, 47, 112, 134, 361, 1082, 1111
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 7 cut(s) 82, 124, 177, 256, 662, 683, 1154
DpnI GATC 7 cut(s) 15, 102, 132, 264, 375, 492, 870
DpnII GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 2 cut(s) 403, 943
Eco130I CCWWGG 4 cut(s) 6, 608, 632, 689
Eco47I GGWCC 2 cut(s) 644, 986
Eco57I CTGAAG 1 cut(s) 354
EcoRII CCWGG 3 cut(s) 640, 767, 1024
EcoT14I CCWWGG 4 cut(s) 6, 608, 632, 689
ErhI CCWWGG 4 cut(s) 6, 608, 632, 689
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 7 cut(s) 10, 50, 115, 137, 364, 1085, 1114
FalI AAGNNNNNCTT 2 cut(s) 880, 912
FatI CATG 7 cut(s) 6, 46, 111, 133, 360, 1081, 1110
FauI CCCGC 1 cut(s) 149
FbaI TGATCA 2 cut(s) 130, 262
FokI GGATG 4 cut(s) 392, 403, 859, 977
FspBI CTAG 6 cut(s) 609, 669, 690, 837, 855, 1070
GlaI GCGC 3 cut(s) 232, 413, 455
HaeIII GGCC 2 cut(s) 5, 637
HhaI GCGC 3 cut(s) 233, 414, 456
Hin1II CATG 7 cut(s) 10, 50, 115, 137, 364, 1085, 1114
Hin6I GCGC 3 cut(s) 231, 412, 454
HinP1I GCGC 3 cut(s) 231, 412, 454
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 2 cut(s) 309, 364
HphI GGTGA 6 cut(s) 228, 642, 985, 1084, 1117, 1166
Hpy166II GTNNAC 2 cut(s) 169, 1174
Hpy188I TCNGA 5 cut(s) 31, 267, 373, 831, 1020
Hpy188III TCNNGA 5 cut(s) 17, 38, 377, 1055, 1132
Hpy8I GTNNAC 2 cut(s) 169, 1174
HpyAV CCTTC 3 cut(s) 378, 657, 1132
HpyCH4III ACNGT 4 cut(s) 23, 406, 419, 1009
HpyCH4V TGCA 4 cut(s) 538, 616, 781, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 705
HpyF3I CTNAG 7 cut(s) 82, 124, 177, 256, 662, 683, 1154
Hsp92II CATG 7 cut(s) 10, 50, 115, 137, 364, 1085, 1114
HspAI GCGC 3 cut(s) 231, 412, 454
Ksp22I TGATCA 2 cut(s) 130, 262
Kzo9I GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
LmnI GCTCC 3 cut(s) 199, 409, 811
LweI GCATC 1 cut(s) 242
MabI ACCWGGT 1 cut(s) 640
MaeI CTAG 6 cut(s) 609, 669, 690, 837, 855, 1070
MaeIII GTNAC 1 cut(s) 77
MalI GATC 7 cut(s) 15, 102, 132, 264, 375, 492, 870
MboI GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
MboII GAAGA 4 cut(s) 53, 539, 876, 1070
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 162, 501, 823, 977, 1074, 1117, 1140
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 866
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1170
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 480, 599, 906, 1116
MslI CAYNNNNRTG 1 cut(s) 444
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 4 cut(s) 260, 326, 588, 1100
MspR9I CCNGG 3 cut(s) 642, 769, 1026
Mva1269I GAATGC 1 cut(s) 1151
MvaI CCWGG 3 cut(s) 642, 769, 1026
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 705
NcoI CCATGG 1 cut(s) 6
NdeII GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 7 cut(s) 10, 50, 115, 137, 364, 1085, 1114
NlaIV GGNNCC 1 cut(s) 987
NmeAIII GCCGAG 2 cut(s) 675, 1186
NruI TCGCGA 1 cut(s) 18
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 1 cut(s) 1151
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 1 cut(s) 364
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 906
PsiI TTATAA 1 cut(s) 753
Psp6I CCWGG 3 cut(s) 640, 767, 1024
PspGI CCWGG 3 cut(s) 640, 767, 1024
PspN4I GGNNCC 1 cut(s) 987
PspPI GGNCC 2 cut(s) 644, 986
PstI CTGCAG 3 cut(s) 540, 618, 805
PvuI CGATCG 1 cut(s) 16
PvuII CAGCTG 3 cut(s) 260, 326, 588
RruI TCGCGA 1 cut(s) 18
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 4 cut(s) 480, 599, 906, 1116
Sau3AI GATC 7 cut(s) 13, 100, 130, 262, 373, 490, 868
Sau96I GGNCC 2 cut(s) 644, 986
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 3 cut(s) 642, 769, 1026
SexAI ACCWGGT 1 cut(s) 640
SfaNI GCATC 1 cut(s) 242
SfcI CTRYAG 4 cut(s) 189, 536, 614, 801
SinI GGWCC 2 cut(s) 644, 986
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 2 cut(s) 311, 442
SmoI CTYRAG 2 cut(s) 311, 442
Sse9I AATT 7 cut(s) 162, 501, 823, 977, 1074, 1117, 1140
SsiI CCGC 7 cut(s) 156, 339, 388, 699, 954, 962, 1098
SspI AATATT 3 cut(s) 777, 795, 814
SspMI CTAG 6 cut(s) 609, 669, 690, 837, 855, 1070
StyD4I CCNGG 3 cut(s) 640, 767, 1024
StyI CCWWGG 4 cut(s) 6, 608, 632, 689
TaaI ACNGT 4 cut(s) 23, 406, 419, 1009
TaqI TCGA 2 cut(s) 39, 918
TaqII GACCGA 1 cut(s) 284
TasI AATT 7 cut(s) 162, 501, 823, 977, 1074, 1117, 1140
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 4 cut(s) 480, 599, 906, 1116
Tru9I TTAA 4 cut(s) 480, 599, 906, 1116
TscAI CASTG 3 cut(s) 349, 411, 1014
TspDTI ATGAA 5 cut(s) 15, 222, 294, 377, 446
TspRI CASTG 3 cut(s) 349, 411, 1014
VpaK11BI GGWCC 2 cut(s) 644, 986
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 2 cut(s) 977, 1074
XceI RCATGY 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 964
XmaJI CCTAGG 2 cut(s) 608, 689
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 6 cut(s) 609, 669, 690, 837, 855, 1070
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.