MD07G1285100.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015416
Physical Location & Seq
Forward (+)
34741331 .. 34743913
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1285100.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGGCCATGGAAGATCACGACCGTTCATCTGATTTTCTCGAAGAACATGAGCAGTACTGCTACAGCGATACCAATTGTAACTTGGGAACTCCGGGGGATGATCTTTTTAGCATATTACAGAGCTTAGATGATCATGGGTTTGGAGGAGGAGGCGGCGGAGTCGTAGACTTTCCTTCCCCGTTAGCTTCAGCACCATTCGATGATGACCAACTACTTGCTGTTTTTAGCTCATCACCAAAAGACCTCTCCTCCTCAGCTGATAAAACCGAGTTTGATGAAACTAACTCTCCACCCAAAACCAAGAGACTCAAGCTCTCATCAGCTGCCACCCCCACCGCCACTGCCACCACAACAACCACAAATTCTTCAGATCAGGATGGAGGGCGGATGTCTCATATAACGGTGGAGCGCAACCGCAGAAAGCAAATGAACGACCACTTGACTGTCTTGCGCTCTCTTATGCCTTGTTTCTATGTTAAACGAGGAGATCAAGCATCAATCATTGGAGGGGTGGTTGATTACATCAATGAGCTGCAGCAAGTTCTTCAATCACTTGAAGCCAAAAAGCAACGAAAAGCATACAGCTGTGAAGTGTTAAGCAGCCCTAGGTTGCAGCAGGTTTCAAGTCCAAGGCCACCTGGTCCCTCACCACCAGTTCTCAGCCCTAGAAAGCCACCTCTCAGCCCTAGGCTCGGCGGCAGCAGCTTACCACCCATCAGCCCAAGAACTCCACAACCCACCAGCCCTTATAAGTCCACGGCAGCCAGGACAATATTGCAACAACCACAAATATTACCTGCAGCTGCTAATATTATTAGTAATTATCTGATTTCTAATCCAACAATGGCTGCTAGCTCATCACTTGAGCCATCCCCTACTTCTTCAAGTGCTACTTCCTCTATTAATAATAGTGTCGACAATCTGAACGAGCTTTTTGCCAACTCCAAGTCCGCCATTGCGGACGTGGAGGTGAAATTTTCCGGTCCAAATGTTCTTTTGAAAACAGTGTCTCCTCCGATACCCGGACAGGCTCTCAAAATCATTTCCGCTCTTGAAGACCTTTCGCTTGAAATTCTCCATGTCAGTGTCACCACCGTTGACGAAACCATGCTTAATTCCTTCACCATCAAGATTGGAATTGAATGCCAACTTAGTGCCGAGGAACTCGCTCACCAAATCCAGCAAACATTCTGCTAA

Protein Analysis

399

Amino Acids

42.73

Weight (kDa)

5.15

Isoelectric Point (pI)

65.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 129 - 179 7.4e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53210 AT5G53210
fragaria_vesca FvH4_7g31020
malus_domestica MD01G1215200.v1.1 MD07G1285100.v1.1
prunus_persica Prupe.2G307300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0380101
rosa_laevigata RLG00000026332
rosa_multiflora Rmu_sc0001232.1_g000008
rosa_roxburghii Rroxscaffold_4G00279700
rosa_rugosa Rorug01G0417400
rosa_samantha Rh1AG442600 Rh1BG397600 Rh1CG411000 Rh1DG428400
rosa_wichuraiana Rw1G038430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 2 cut(s) 607, 805
AccBSI CCGCTC 1 cut(s) 1049
AccI GTMKAC 2 cut(s) 165, 915
AciI CCGC 9 cut(s) 153, 156, 336, 385, 415, 696, 951, 959, 1047
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 361, 974, 1071
AcuI CTGAAG 2 cut(s) 171, 351
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 692, 1022
AgsI TTSAA 8 cut(s) 548, 557, 624, 885, 1000, 1055, 1070, 1142
AjiI CACGTC 1 cut(s) 964
AjnI CCWGG 2 cut(s) 637, 764
Alw26I GTCTC 3 cut(s) 298, 396, 1014
AoxI GGCC 2 cut(s) 3, 632
ApoI RAATTY 3 cut(s) 361, 974, 1071
AseI ATTAAT 1 cut(s) 903
AspA2I CCTAGG 2 cut(s) 605, 686
AspLEI GCGC 2 cut(s) 411, 453
AspS9I GGNCC 2 cut(s) 641, 983
AsuC2I CCSGG 2 cut(s) 93, 1023
AsuHPI GGTGA 6 cut(s) 225, 639, 982, 1081, 1114, 1163
AsuNHI GCTAGC 1 cut(s) 851
AvaII GGWCC 2 cut(s) 641, 983
AvrII CCTAGG 2 cut(s) 605, 686
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 1062
BbvCI CCTCAGC 1 cut(s) 253
BccI CCATC 4 cut(s) 371, 722, 877, 1133
BceAI ACGGC 1 cut(s) 774
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BciT130I CCWGG 2 cut(s) 639, 766
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 2 cut(s) 93, 1023
BcoDI GTCTC 3 cut(s) 298, 396, 1014
BfaI CTAG 4 cut(s) 606, 666, 687, 852
BfmI CTRYAG 3 cut(s) 61, 533, 798
BfuAI ACCTGC 2 cut(s) 607, 805
BlnI CCTAGG 2 cut(s) 605, 686
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 4 cut(s) 93, 639, 766, 1023
Bme18I GGWCC 2 cut(s) 641, 983
BmgBI CACGTC 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 641, 983
BmiI GGNNCC 1 cut(s) 643
BmrFI CCNGG 4 cut(s) 93, 639, 766, 1023
BmsI GCATC 1 cut(s) 503
BmtI GCTAGC 1 cut(s) 855
BpiI GAAGAC 1 cut(s) 1062
Bpu10I CCTNAGC 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 293, 884
BpuMI CCSGG 2 cut(s) 93, 1023
BsaJI CCNNGG 7 cut(s) 6, 92, 605, 629, 686, 756, 1158
BsaWI WCCGGW 1 cut(s) 980
BsaXI ACNNNNNCTCC 8 cut(s) 233, 263, 271, 301, 498, 528, 994, 1024
Bsc4I CCNNNNNNNGG 2 cut(s) 692, 1022
Bse1I ACTGG 1 cut(s) 653
Bse3DI GCAATG 1 cut(s) 954
BseBI CCWGG 2 cut(s) 639, 766
BseDI CCNNGG 7 cut(s) 6, 92, 605, 629, 686, 756, 1158
BseGI GGATG 4 cut(s) 103, 382, 393, 869
BseLI CCNNNNNNNGG 2 cut(s) 692, 1022
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 3 cut(s) 267, 673, 694
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 6 cut(s) 159, 162, 238, 241, 498, 1002
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 5, 634
BsiEI CGRYCG 1 cut(s) 22
BsiSI CCGG 3 cut(s) 92, 981, 1023
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 2 cut(s) 692, 1022
BsmAI GTCTC 3 cut(s) 298, 396, 1014
BsmFI GGGAC 1 cut(s) 627
BsmI GAATGC 1 cut(s) 1148
BsnI GGCC 2 cut(s) 5, 634
Bsp143I GATC 5 cut(s) 13, 100, 130, 370, 487
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 9 cut(s) 153, 156, 336, 385, 415, 696, 951, 959, 1047
BspANI GGCC 2 cut(s) 5, 634
BspCNI CTCAG 3 cut(s) 266, 672, 693
BspLI GGNNCC 1 cut(s) 643
BspMAI CTGCAG 2 cut(s) 537, 802
BspMI ACCTGC 2 cut(s) 607, 805
BspOI GCTAGC 1 cut(s) 855
BsrBI CCGCTC 1 cut(s) 1049
BsrDI GCAATG 1 cut(s) 954
BsrI ACTGG 1 cut(s) 653
BssECI CCNNGG 7 cut(s) 6, 92, 605, 629, 686, 756, 1158
BssMI GATC 5 cut(s) 13, 100, 130, 370, 487
BssT1I CCWWGG 4 cut(s) 6, 605, 629, 686
Bst2UI CCWGG 2 cut(s) 639, 766
Bst4CI ACNGT 5 cut(s) 23, 403, 445, 1006, 1096
BstC8I GCNNGC 1 cut(s) 853
BstDEI CTNAG 5 cut(s) 124, 253, 659, 680, 1151
BstDSI CCRYGG 2 cut(s) 6, 756
BstF5I GGATG 4 cut(s) 103, 382, 393, 869
BstHHI GCGC 2 cut(s) 411, 453
BstKTI GATC 5 cut(s) 16, 103, 133, 373, 490
BstMAI GTCTC 3 cut(s) 298, 396, 1014
BstMBI GATC 5 cut(s) 13, 100, 130, 370, 487
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 702
BstNI CCWGG 2 cut(s) 639, 766
BstSCI CCNGG 4 cut(s) 91, 637, 764, 1021
BstSFI CTRYAG 3 cut(s) 61, 533, 798
BstV2I GAAGAC 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 5, 634
BtgI CCRYGG 2 cut(s) 6, 756
BtrI CACGTC 1 cut(s) 964
BtsCI GGATG 4 cut(s) 103, 382, 393, 869
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 339, 1011, 1090
BveI ACCTGC 2 cut(s) 607, 805
Cac8I GCNNGC 1 cut(s) 853
CfoI GCGC 2 cut(s) 411, 453
Cfr13I GGNCC 2 cut(s) 641, 983
CsiI ACCWGGT 1 cut(s) 637
Csp6I GTAC 1 cut(s) 55
CviAII CATG 5 cut(s) 7, 47, 134, 1079, 1108
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 5 cut(s) 124, 253, 659, 680, 1151
DpnI GATC 5 cut(s) 15, 102, 132, 372, 489
DpnII GATC 5 cut(s) 13, 100, 130, 370, 487
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 3 cut(s) 171, 400, 940
Eco130I CCWWGG 4 cut(s) 6, 605, 629, 686
Eco47I GGWCC 2 cut(s) 641, 983
Eco57I CTGAAG 2 cut(s) 171, 351
EcoRII CCWGG 2 cut(s) 637, 764
EcoT14I CCWWGG 4 cut(s) 6, 605, 629, 686
ErhI CCWWGG 4 cut(s) 6, 605, 629, 686
FaeI CATG 5 cut(s) 10, 50, 137, 1082, 1111
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 1 cut(s) 627
FatI CATG 5 cut(s) 6, 46, 133, 1078, 1107
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 2 cut(s) 165, 915
FokI GGATG 4 cut(s) 110, 389, 400, 856
FspBI CTAG 4 cut(s) 606, 666, 687, 852
GlaI GCGC 2 cut(s) 410, 452
HaeIII GGCC 2 cut(s) 5, 634
HapII CCGG 3 cut(s) 92, 981, 1023
HhaI GCGC 2 cut(s) 411, 453
Hin1II CATG 5 cut(s) 10, 50, 137, 1082, 1111
Hin6I GCGC 2 cut(s) 409, 451
HinP1I GCGC 2 cut(s) 409, 451
HincII GTYRAC 2 cut(s) 916, 1099
HindII GTYRAC 2 cut(s) 916, 1099
HinfI GANTC 2 cut(s) 159, 306
HpaII CCGG 3 cut(s) 92, 981, 1023
HphI GGTGA 6 cut(s) 225, 639, 982, 1081, 1114, 1163
Hpy166II GTNNAC 4 cut(s) 166, 756, 916, 1099
Hpy188I TCNGA 5 cut(s) 31, 370, 828, 924, 1017
Hpy188III TCNNGA 5 cut(s) 17, 38, 374, 1052, 1129
Hpy8I GTNNAC 4 cut(s) 166, 756, 916, 1099
HpyAV CCTTC 2 cut(s) 183, 1129
HpyCH4III ACNGT 5 cut(s) 23, 403, 445, 1006, 1096
HpyCH4IV ACGT 1 cut(s) 963
HpyCH4V TGCA 4 cut(s) 535, 613, 778, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 702
HpyF3I CTNAG 5 cut(s) 124, 253, 659, 680, 1151
HpySE526I ACGT 1 cut(s) 963
Hsp92II CATG 5 cut(s) 10, 50, 137, 1082, 1111
HspAI GCGC 2 cut(s) 409, 451
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 5 cut(s) 13, 100, 130, 370, 487
LmnI GCTCC 1 cut(s) 406
LweI GCATC 1 cut(s) 503
MabI ACCWGGT 1 cut(s) 637
MaeI CTAG 4 cut(s) 606, 666, 687, 852
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 2 cut(s) 77, 1087
MalI GATC 5 cut(s) 15, 102, 132, 372, 489
MbiI CCGCTC 1 cut(s) 1049
MboI GATC 5 cut(s) 13, 100, 130, 370, 487
MboII GAAGA 6 cut(s) 23, 53, 357, 536, 873, 1067
MfeI CAATTG 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 73, 361, 820, 974, 1071, 1114, 1137
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 168, 300
MmeI TCCRAC 1 cut(s) 863
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 477, 596, 903, 1113
MslI CAYNNNNRTG 1 cut(s) 1083
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 4 cut(s) 257, 323, 585, 803
MspI CCGG 3 cut(s) 92, 981, 1023
MspR9I CCNGG 4 cut(s) 93, 639, 766, 1023
MunI CAATTG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 1148
MvaI CCWGG 2 cut(s) 639, 766
MwoI GCNNNNNNNGC 1 cut(s) 702
NciI CCSGG 2 cut(s) 93, 1023
NcoI CCATGG 1 cut(s) 6
NdeII GATC 5 cut(s) 13, 100, 130, 370, 487
NheI GCTAGC 1 cut(s) 851
NlaIII CATG 5 cut(s) 10, 50, 137, 1082, 1111
NlaIV GGNNCC 1 cut(s) 643
NmeAIII GCCGAG 2 cut(s) 672, 1183
NmuCI GTSAC 1 cut(s) 1087
PctI GAATGC 1 cut(s) 1148
PleI GAGTC 2 cut(s) 167, 300
PpsI GAGTC 2 cut(s) 167, 300
PshBI ATTAAT 1 cut(s) 903
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 637, 764
PspGI CCWGG 2 cut(s) 637, 764
PspN4I GGNNCC 1 cut(s) 643
PspPI GGNCC 2 cut(s) 641, 983
PstI CTGCAG 2 cut(s) 537, 802
PvuII CAGCTG 4 cut(s) 257, 323, 585, 803
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 1083
SalI GTCGAC 1 cut(s) 914
SaqAI TTAA 4 cut(s) 477, 596, 903, 1113
Sau3AI GATC 5 cut(s) 13, 100, 130, 370, 487
Sau96I GGNCC 2 cut(s) 641, 983
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 2 cut(s) 168, 300
ScrFI CCNGG 4 cut(s) 93, 639, 766, 1023
SexAI ACCWGGT 1 cut(s) 637
SfaNI GCATC 1 cut(s) 503
SfcI CTRYAG 3 cut(s) 61, 533, 798
SinI GGWCC 2 cut(s) 641, 983
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 2 cut(s) 308, 863
SmoI CTYRAG 2 cut(s) 308, 863
Sse9I AATT 7 cut(s) 73, 361, 820, 974, 1071, 1114, 1137
SsiI CCGC 9 cut(s) 153, 156, 336, 385, 415, 696, 951, 959, 1047
SspI AATATT 3 cut(s) 774, 792, 811
SspMI CTAG 4 cut(s) 606, 666, 687, 852
StyD4I CCNGG 4 cut(s) 91, 637, 764, 1021
StyI CCWWGG 4 cut(s) 6, 605, 629, 686
TaaI ACNGT 5 cut(s) 23, 403, 445, 1006, 1096
TaiI ACGT 1 cut(s) 966
TaqI TCGA 3 cut(s) 39, 198, 915
TasI AATT 7 cut(s) 73, 361, 820, 974, 1071, 1114, 1137
TatI WGTACW 1 cut(s) 54
TauI GCSGC 2 cut(s) 156, 699
Tru1I TTAA 4 cut(s) 477, 596, 903, 1113
Tru9I TTAA 4 cut(s) 477, 596, 903, 1113
TscAI CASTG 3 cut(s) 346, 1011, 1090
TseFI GTSAC 1 cut(s) 1087
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 3 cut(s) 15, 291, 443
TspRI CASTG 3 cut(s) 346, 1011, 1090
VpaK11BI GGWCC 2 cut(s) 641, 983
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 3 cut(s) 361, 974, 1071
XcmI CCANNNNNNNNNTGG 2 cut(s) 79, 961
XmaJI CCTAGG 2 cut(s) 605, 686
XmiI GTMKAC 2 cut(s) 165, 915
XspI CTAG 4 cut(s) 606, 666, 687, 852
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.