MD01G1234300.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009939
Physical Location & Seq
Reverse (-)
32200594 .. 32203791
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1234300.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGGTCAAGCTTTTCGCAAGCTCTTCGACACCTTCTTCGGCAACACGGAGATGCGAGTTGTAATGCTCGGCCTCGACGCAGCTGGTAAAACAACCATCCTTTACAAGCTGCACATTGGAGAAGTTTTGTCAACTGTTCCTACAATAGGTTTCAATGTGGAGAAAGTTCAATACAAGAATGTGATGTTCACTGTTTGGGATGTCGGTGGACAAGAGAAACTAAGGCCACTCTGGAGGCATTACTTTAATAACACGGACGGACTGATTTACGTTGTTGATTCCTTGGACAGAGAGAGAATTGGAAGAGCAAAGGCAGAATTTCAGGTTGCCATCATCAGTGATCCATTTATGCGCAACAGTGTCATCTTGGTGTTTGCTAATAAACAGGATATGAAAGGAGCCATGTCGCCAATGGAAGTGTGTGAAGGACTAGGCCTCTATGATCTCAAGAACAGAAAATGGCACATACAAGGGACTTGTGCCCTTAGAGGTGATGGCCTTTACGAAGGCTTGGATTGGTTATCTAGCACGCTGAAAGAGATGAGAGCTGCTGGATACTCTTCAGTGGGCACCTCGTTTATTAGGATTCTTGGGTCTGTCAATCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

204

Amino Acids

22.9

Weight (kDa)

8.83

Isoelectric Point (pI)

31.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 6 - 177 5.4e-70 ADP-ribosylation factor family
SRPRB PF09439 17 - 141 6.1e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 130 3.3e-12 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 133 8.3e-10 Ras family
Gtr1_RagA PF04670 20 - 131 1.2e-07 Gtr1/RagA G protein conserved region
G-alpha PF00503 44 - 130 1.8e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 569
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 546
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 2 cut(s) 154, 170
AluBI AGCT 5 cut(s) 11, 22, 83, 109, 548
AluI AGCT 5 cut(s) 11, 22, 83, 109, 548
AlwI GGATC 1 cut(s) 335
AoxI GGCC 4 cut(s) 70, 224, 433, 496
ApeKI GCWGC 3 cut(s) 80, 109, 548
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AspLEI GCGC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 503
BaeGI GKGCMC 2 cut(s) 484, 572
BanI GGYRCC 1 cut(s) 569
BbvI GCAGC 3 cut(s) 92, 96, 535
BccI CCATC 3 cut(s) 104, 338, 488
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciVI GTATCC 1 cut(s) 548
BfaI CTAG 2 cut(s) 431, 525
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 3 cut(s) 81, 110, 549
BlsI GCNGC 3 cut(s) 82, 111, 550
BmiI GGNNCC 2 cut(s) 400, 571
BmsI GCATC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 2 cut(s) 96, 205
BseLI CCNNNNNNNGG 1 cut(s) 146
BseSI GKGCMC 2 cut(s) 484, 572
BseXI GCAGC 3 cut(s) 92, 96, 535
BsgI GTGCAG 1 cut(s) 95
BshFI GGCC 4 cut(s) 72, 226, 435, 498
BshNI GGYRCC 1 cut(s) 569
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 4 cut(s) 72, 226, 435, 498
Bsp1286I GDGCHC 2 cut(s) 484, 572
Bsp143I GATC 2 cut(s) 340, 442
BspANI GGCC 4 cut(s) 72, 226, 435, 498
BspLI GGNNCC 2 cut(s) 400, 571
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 2 cut(s) 29, 298
BspT107I GGYRCC 1 cut(s) 569
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 2 cut(s) 340, 442
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 3 cut(s) 136, 193, 359
Bst6I CTCTTC 3 cut(s) 29, 298, 565
BstC8I GCNNGC 2 cut(s) 20, 530
BstDEI CTNAG 2 cut(s) 221, 485
BstENI CCTNNNNNAGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 96, 205
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 2 cut(s) 343, 445
BstMBI GATC 2 cut(s) 340, 442
BstSLI GKGCMC 2 cut(s) 484, 572
BstV1I GCAGC 3 cut(s) 92, 96, 535
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 4 cut(s) 72, 226, 435, 498
BtsCI GGATG 2 cut(s) 96, 205
BtsIMutI CAGTG 4 cut(s) 189, 343, 364, 570
Cac8I GCNNGC 2 cut(s) 20, 530
CfoI GCGC 1 cut(s) 354
CseI GACGC 1 cut(s) 86
CviAII CATG 1 cut(s) 403
DdeI CTNAG 2 cut(s) 221, 485
DpnI GATC 2 cut(s) 342, 444
DpnII GATC 2 cut(s) 340, 442
Eam1104I CTCTTC 3 cut(s) 29, 298, 565
EarI CTCTTC 3 cut(s) 29, 298, 565
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 546
EcoNI CCTNNNNNAGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 1 cut(s) 406
FaiI YATR 5 cut(s) 350, 392, 404, 441, 467
FaqI GGGAC 1 cut(s) 487
FatI CATG 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 81, 110, 549
FokI GGATG 2 cut(s) 83, 212
Fsp4HI GCNGC 3 cut(s) 81, 110, 549
FspBI CTAG 2 cut(s) 431, 525
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 1 cut(s) 353
GluI GCNGC 3 cut(s) 81, 110, 549
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 4 cut(s) 72, 226, 435, 498
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 354
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 9
HinfI GANTC 2 cut(s) 278, 586
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 3 cut(s) 132, 189, 209
Hpy188III TCNNGA 2 cut(s) 232, 448
Hpy8I GTNNAC 3 cut(s) 132, 189, 209
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 43, 419, 500
HpyCH4III ACNGT 3 cut(s) 136, 193, 359
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 112
HpyF3I CTNAG 2 cut(s) 221, 485
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 1 cut(s) 352
Kzo9I GATC 2 cut(s) 340, 442
LguI GCTCTTC 2 cut(s) 29, 298
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 5 cut(s) 69, 217, 308, 371, 537
Lsp1109I GCAGC 3 cut(s) 92, 96, 535
LweI GCATC 1 cut(s) 42
MaeI CTAG 2 cut(s) 431, 525
MaeII ACGT 1 cut(s) 270
MalI GATC 2 cut(s) 342, 444
MboI GATC 2 cut(s) 340, 442
MboII GAAGA 4 cut(s) 16, 28, 315, 552
MhlI GDGCHC 2 cut(s) 484, 572
MluCI AATT 3 cut(s) 297, 317, 607
MnlI CCTC 5 cut(s) 83, 228, 446, 482, 583
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 50, 368
MspA1I CMGCKG 1 cut(s) 83
NdeII GATC 2 cut(s) 340, 442
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 400, 571
NmeAIII GCCGAG 1 cut(s) 48
NsbI TGCGCA 1 cut(s) 353
PceI AGGCCT 1 cut(s) 435
PciSI GCTCTTC 2 cut(s) 29, 298
PfeI GAWTC 2 cut(s) 278, 586
PkrI GCNGC 3 cut(s) 82, 111, 550
PspN4I GGNNCC 2 cut(s) 400, 571
PvuII CAGCTG 1 cut(s) 83
RseI CAYNNNNRTG 2 cut(s) 50, 368
SapI GCTCTTC 2 cut(s) 29, 298
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 3 cut(s) 81, 110, 549
Sau3AI GATC 2 cut(s) 340, 442
SduI GDGCHC 2 cut(s) 484, 572
SfaNI GCATC 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 50, 368
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 3 cut(s) 297, 317, 607
SseBI AGGCCT 1 cut(s) 435
SspMI CTAG 2 cut(s) 431, 525
StuI AGGCCT 1 cut(s) 435
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 3 cut(s) 136, 193, 359
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 27, 75
TasI AATT 3 cut(s) 297, 317, 607
TfiI GAWTC 2 cut(s) 278, 586
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 4 cut(s) 196, 343, 364, 570
TseI GCWGC 3 cut(s) 80, 109, 548
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 3 cut(s) 62, 269, 273
TspRI CASTG 4 cut(s) 196, 343, 364, 570
XagI CCTNNNNNAGG 1 cut(s) 144
XapI RAATTY 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XspI CTAG 2 cut(s) 431, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.