MD07G1306800.v1.1

ARF-like small GTPases; ARF, ADP-ribosylation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009939
Physical Location & Seq
Reverse (-)
36322514 .. 36325256
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1306800.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGGTCAAGCTTTTCGGAAGCTCTTCGACACCTTCTTCGGCAACACTGAGATGCGAGTTGTAATGCTTGGCCTCGACGCAGCTGGTAAAACAACCATCCTTTACAAGCTGCACATTGGTGAAGTTTTGTCAACTGTCCCTACAATAGGTTTCAATGTGGAGAAAATTCAGTATAAGAATGTGATGTTCACTGTTTGGGATGTCGGTGGACAAGAGAAACTAAGGCCACTCTGGAGGCATTACTTTAATAACACGGACGGGCTGATTTACGTTGTTGATTCCTTGGACAGAGAGAGAATTAGAAGAGCGAAGGAGGAATTTCAGGTTGCCATCATCAGTGATCCTTTTATGCTCAGCAGTGTCATCTTGGTGTTTGCTAATAAACAGGATATGAAAGGAGCTATGTCGCCAATGGAAGTGTGTGAAGGACTAGGCCTATTTGATCTCAAGAACAGAAAATGGCACATACAAGGGACTTGTGCCCTTCGAGGCGATGGCCTTTACGAGGGCTTGGATTGGTTAGCTAGCACGCTGAAAGAGATGAGAGCTGCTGGATACTCTTCAGTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

191

Amino Acids

21.65

Weight (kDa)

7.75

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 6 - 177 1.2e-68 ADP-ribosylation factor family
SRPRB PF09439 17 - 141 2.3e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 130 3.6e-12 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 133 1.3e-09 Ras family
Gtr1_RagA PF04670 20 - 112 1.7e-07 Gtr1/RagA G protein conserved region
G-alpha PF00503 44 - 130 1.6e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 2 cut(s) 165, 317
AcuI CTGAAG 1 cut(s) 546
AfiI CCNNNNNNNGG 2 cut(s) 146, 505
AgsI TTSAA 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 117
AluBI AGCT 7 cut(s) 11, 22, 83, 109, 401, 524, 548
AluI AGCT 7 cut(s) 11, 22, 83, 109, 401, 524, 548
AlwI GGATC 1 cut(s) 335
AoxI GGCC 4 cut(s) 70, 224, 433, 496
ApeKI GCWGC 3 cut(s) 80, 109, 548
ApoI RAATTY 2 cut(s) 165, 317
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
Asp700I GAANNNNTTC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 484
BbvI GCAGC 3 cut(s) 92, 96, 535
BccI CCATC 3 cut(s) 104, 338, 488
BciVI GTATCC 1 cut(s) 548
BfaI CTAG 2 cut(s) 431, 525
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 3 cut(s) 81, 110, 549
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 3 cut(s) 82, 111, 550
BmsI GCATC 1 cut(s) 42
BmtI GCTAGC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 253
Bpu1102I GCTNAGC 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 505
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 2 cut(s) 96, 205
BseLI CCNNNNNNNGG 2 cut(s) 146, 505
BseMII CTCAG 2 cut(s) 39, 367
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 3 cut(s) 92, 96, 535
BsgI GTGCAG 1 cut(s) 95
BshFI GGCC 4 cut(s) 72, 226, 435, 498
BslFI GGGAC 2 cut(s) 122, 487
BslI CCNNNNNNNGG 2 cut(s) 146, 505
BsmFI GGGAC 2 cut(s) 122, 487
BsnI GGCC 4 cut(s) 72, 226, 435, 498
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 2 cut(s) 340, 442
Bsp1720I GCTNAGC 1 cut(s) 353
BspANI GGCC 4 cut(s) 72, 226, 435, 498
BspCNI CTCAG 2 cut(s) 40, 366
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 2 cut(s) 29, 298
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 2 cut(s) 340, 442
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 136, 193
Bst6I CTCTTC 3 cut(s) 29, 298, 565
BstC8I GCNNGC 2 cut(s) 526, 530
BstDEI CTNAG 3 cut(s) 48, 221, 353
BstENI CCTNNNNNAGG 2 cut(s) 144, 503
BstF5I GGATG 2 cut(s) 96, 205
BstKTI GATC 2 cut(s) 343, 445
BstMBI GATC 2 cut(s) 340, 442
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 92, 96, 535
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 4 cut(s) 72, 226, 435, 498
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 2 cut(s) 96, 205
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 4 cut(s) 45, 189, 343, 364
Cac8I GCNNGC 2 cut(s) 526, 530
CseI GACGC 1 cut(s) 86
DdeI CTNAG 3 cut(s) 48, 221, 353
DpnI GATC 2 cut(s) 342, 444
DpnII GATC 2 cut(s) 340, 442
Eam1104I CTCTTC 3 cut(s) 29, 298, 565
EarI CTCTTC 3 cut(s) 29, 298, 565
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 546
EcoNI CCTNNNNNAGG 2 cut(s) 144, 503
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaiI YATR 5 cut(s) 174, 350, 392, 404, 467
FaqI GGGAC 2 cut(s) 122, 487
Fnu4HI GCNGC 3 cut(s) 81, 110, 549
FokI GGATG 2 cut(s) 83, 212
Fsp4HI GCNGC 3 cut(s) 81, 110, 549
FspBI CTAG 2 cut(s) 431, 525
GluI GCNGC 3 cut(s) 81, 110, 549
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 4 cut(s) 72, 226, 435, 498
HgaI GACGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 9
HinfI GANTC 1 cut(s) 278
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 132, 189, 209
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 232, 448
Hpy8I GTNNAC 3 cut(s) 132, 189, 209
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 4 cut(s) 43, 304, 419, 494
HpyCH4III ACNGT 2 cut(s) 136, 193
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 112
HpyF3I CTNAG 3 cut(s) 48, 221, 353
HpySE526I ACGT 1 cut(s) 270
Kzo9I GATC 2 cut(s) 340, 442
LguI GCTCTTC 2 cut(s) 29, 298
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 5 cut(s) 69, 217, 308, 371, 537
Lsp1109I GCAGC 3 cut(s) 92, 96, 535
LweI GCATC 1 cut(s) 42
MaeI CTAG 2 cut(s) 431, 525
MaeII ACGT 1 cut(s) 270
MalI GATC 2 cut(s) 342, 444
MboI GATC 2 cut(s) 340, 442
MboII GAAGA 4 cut(s) 16, 28, 315, 552
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 3 cut(s) 165, 297, 317
MnlI CCTC 5 cut(s) 83, 228, 307, 482, 499
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 3 cut(s) 50, 117, 368
MspA1I CMGCKG 1 cut(s) 83
NdeII GATC 2 cut(s) 340, 442
NheI GCTAGC 1 cut(s) 524
OliI CACNNNNGTG 1 cut(s) 117
PceI AGGCCT 1 cut(s) 435
PciSI GCTCTTC 2 cut(s) 29, 298
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 3 cut(s) 82, 111, 550
PvuII CAGCTG 1 cut(s) 83
RseI CAYNNNNRTG 3 cut(s) 50, 117, 368
SapI GCTCTTC 2 cut(s) 29, 298
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 3 cut(s) 81, 110, 549
Sau3AI GATC 2 cut(s) 340, 442
SduI GDGCHC 1 cut(s) 484
SfaNI GCATC 1 cut(s) 42
SmiMI CAYNNNNRTG 3 cut(s) 50, 117, 368
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 3 cut(s) 165, 297, 317
SseBI AGGCCT 1 cut(s) 435
SspMI CTAG 2 cut(s) 431, 525
StuI AGGCCT 1 cut(s) 435
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 136, 193
TaiI ACGT 1 cut(s) 273
TaqI TCGA 3 cut(s) 27, 75, 487
TasI AATT 3 cut(s) 165, 297, 317
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 4 cut(s) 52, 196, 343, 364
TseI GCWGC 3 cut(s) 80, 109, 548
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 4 cut(s) 52, 196, 343, 364
XagI CCTNNNNNAGG 2 cut(s) 144, 503
XapI RAATTY 2 cut(s) 165, 317
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 431, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.