Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007974

Physical Location & Seq

Genomic Coordinates Reverse (-)

32480646 .. 32484522

Gene Structure 3877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1238500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGACCATGCTGAGCTCACGACTGAACAGGTTTTGAATAGGGACATCCCATGGGAGACTTACATGACCACTAAGCTCATCACTGGAACCTGCCTTCAGCTCTTGAGGCGCTATGATAAAAGATCCGAGAGCTACAGATCACAGCTTCTCGATGATGATGGCCCTGCTTATGTTCAAGTGTTCATTGGCATTTTACGTGATATTTTCAAGGAAGAAACGGTAGAATATGTCCTTGCTTTAATTGATGAAATGCTTACAGCTAACCCTAAAAGAGCAAGATTGTTCCATGATAGTAGTCTTGCTGATAAAGATATCTATGAGCCTTTCTTGAGATTGCTCTGGAAGGGTAATTGGTTCATACAAGAGAAGAGCTGTAAGATACTTGCTGTAATAGTGAGTGCCAGGCCAAAACCCCAAGATGGCTCTGCTGCAAATGGAGAAGCCTCAAATTCAAAGAGAAAAATAACTACTATTGATGATGTGTTGAAAGGATTGGTGGAATGGCTTTGTGCACAGTTGAAGAAGCCTTCCCATCCTAGTCGTGGTATCCCAACTGCCATCAATTGCCTTGCAACCTTACTAAAGGAACCTATAGTTAGATCTTCCTTTGTTCAATTAGATGGGGTGAAGTTGCTTGTTCCCTTAATTTCTCCAGCTTCCACCCAACAATCTATTCAGCTTCTTTATGAAACATGTCTATGCATCTGGCTCTTATCTTATTATGAACCTGCAATTGAGTACTTGGCTACTTCTAGAACTCTTCCACGACTCATAGAAGTTGTCAAGAGTTCCACAAAGGAGAAGGTTGTCAGAGTTGTTGTTTTGACCCTGAGGAACTTGCTCTCAAAAGGGGCATTTGGCGCTCAAATGGTGGACCTTGGACTGCCGCAAATCGTTCAGAATTTGAAAGCACAAGCATGGAGTGATGAGGATCTCCTGGAGGGATTAAATCAACTGGAAGAGGGTCTGAAGGATAACATCAAGAAATTAAGTTCTTTTGACAAGTATAAGCAAGAAGTCCTCCTTGGCCATCTTGACTGGTCACCCATGCACAAGGATCCAATATTCTGGCGAGAGAATATTACCAACTTTGAAGATAATGACTTTCAGATTCTAAGAGTCCTGATTACTATCTTGGACACATCCAGTGATCCTAGGGCATTGGCCGTAGCCTGCTTTGATATCTCACAGTTTGTCCAGCAGCATCCGGCTGGAAGAAGCATAGTGACAGACCTCAAAGCCAAGGAACGTGTGATGAAACTGATGAATCATGAGAGTGCCGAGGTCACCAAAAATGCGCTGCTGTGCATCCAAAGGCTTTTCCTAGGTGCCAAGTATGCTAGCTTTTTGCAGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000221 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008324 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0032991 GO:0033176 GO:0033178 GO:0033180 GO:0034220 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046961 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1902600

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04142 ko04145 ko04150 ko04721 ko05110 ko05120 ko05152 ko05323 map00190 map01100 map04142 map04145 map04150 map04721 map05110 map05120 map05152 map05323

Pfam Domains

Protein Families

V-ATPase_H_C V-ATPase_H_N

Protein Analysis

453

Amino Acids

51.31

Weight (kDa)

6.68

Isoelectric Point (pI)

43.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
V-ATPase_H_N	PF03224	3 - 323	7.4e-86	V-ATPase subunit H
V-ATPase_H_C	PF11698	329 - 441	5.7e-41	V-ATPase subunit H

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42050
fragaria_vesca	FvH4_5g39390 FvH4_7g34170 FvH4_7g34170 FvH4_7g34170 FvH4_7g34170
malus_domestica	MD01G1238500.v1.1 MD07G1310400.v1.1
prunus_persica	Prupe.2G328400_v2.0.a1 Prupe.2G328400_v2.0.a1
pyrus_communis	pycom01g24610 pycom07g28260
rosa_chinensis	RchiOBHm_Chr1g0384521
rosa_laevigata	RLG00000026035
rosa_multiflora	Rmu_co8438647.1_g000001 Rmu_sc0006005.1_g000006
rosa_roxburghii	Rroxscaffold_4G00276630
rosa_rugosa	Rorug01G0448500 Rorug01G0448600
rosa_samantha	Rh1AG473700 Rh1BG431800 Rh1CG444900 Rh1DG464300
rosa_wichuraiana	Rw1G039890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	98, 736
AccB1I	GGYRCC	1 cut(s)	1328
AciI	CCGC	1 cut(s)	887
AclWI	GGATC	5 cut(s)	117, 939, 1052, 1065, 1145
AcoI	YGGCCR	2 cut(s)	1027, 1164
AcsI	RAATTY	2 cut(s)	448, 901
AcuI	CTGAAG	2 cut(s)	80, 989
AfaI	GTAC	1 cut(s)	740
AfiI	CCNNNNNNNGG	2 cut(s)	419, 542
AflIII	ACRYGT	2 cut(s)	692, 1249
AgsI	TTSAA	9 cut(s)	37, 176, 208, 453, 487, 520, 614, 907, 1094
AjnI	CCWGG	2 cut(s)	401, 936
AjuI	GAANNNNNNNTTGG	4 cut(s)	657, 689, 1008, 1040
Alw21I	GWGCWC	2 cut(s)	18, 514
Alw26I	GTCTC	1 cut(s)	50
Alw44I	GTGCAC	1 cut(s)	510
AlwI	GGATC	5 cut(s)	117, 939, 1052, 1065, 1145
AoxI	GGCC	4 cut(s)	160, 404, 1027, 1164
ApaLI	GTGCAC	1 cut(s)	510
ApeKI	GCWGC	3 cut(s)	428, 1201, 1300
ApoI	RAATTY	2 cut(s)	448, 901
AspA2I	CCTAGG	2 cut(s)	1154, 1324
AspLEI	GCGC	3 cut(s)	111, 863, 1300
AspS9I	GGNCC	3 cut(s)	4, 161, 874
AsuHPI	GGTGA	3 cut(s)	637, 1035, 1279
AsuNHI	GCTAGC	1 cut(s)	1340
AvaII	GGWCC	2 cut(s)	4, 874
AvrII	CCTAGG	2 cut(s)	1154, 1324
AxyI	CCTNAGG	1 cut(s)	830
BaeGI	GKGCMC	1 cut(s)	514
BalI	TGGCCA	1 cut(s)	1029
BamHI	GGATCC	1 cut(s)	1057
BanI	GGYRCC	1 cut(s)	1328
BanII	GRGCYC	1 cut(s)	18
Bbv12I	GWGCWC	2 cut(s)	18, 514
BbvI	GCAGC	3 cut(s)	415, 1213, 1287
BccI	CCATC	6 cut(s)	152, 413, 540, 566, 614, 1038
BceAI	ACGGC	1 cut(s)	1151
BciT130I	CCWGG	2 cut(s)	403, 938
BciVI	GTATCC	1 cut(s)	557
BcoDI	GTCTC	1 cut(s)	50
BfaI	CTAG	5 cut(s)	537, 753, 1155, 1325, 1341
BfmI	CTRYAG	2 cut(s)	133, 591
BfoI	RGCGCY	2 cut(s)	112, 864
BfuAI	ACCTGC	2 cut(s)	98, 736
BfuI	GTATCC	1 cut(s)	557
BglII	AGATCT	1 cut(s)	599
BisI	GCNGC	4 cut(s)	429, 887, 1202, 1301
BlnI	CCTAGG	2 cut(s)	1154, 1324
BlpI	GCTNAGC	1 cut(s)	12
BlsI	GCNGC	4 cut(s)	430, 888, 1203, 1302
BmcAI	AGTACT	1 cut(s)	740
Bme1390I	CCNGG	2 cut(s)	403, 938
Bme18I	GGWCC	2 cut(s)	4, 874
BmgT120I	GGNCC	3 cut(s)	4, 161, 874
BmiI	GGNNCC	4 cut(s)	88, 588, 1059, 1330
BmrFI	CCNGG	2 cut(s)	403, 938
BmsI	GCATC	3 cut(s)	711, 1213, 1317
BmtI	GCTAGC	1 cut(s)	1344
BpmI	CTGGAG	2 cut(s)	636, 959
Bpu1102I	GCTNAGC	1 cut(s)	12
BpuEI	CTTGAG	2 cut(s)	124, 349
BsaAI	YACGTR	1 cut(s)	197
BsaBI	GATNNNNATC	2 cut(s)	930, 1130
BsaJI	CCNNGG	7 cut(s)	50, 877, 1024, 1154, 1242, 1281, 1324
Bsc4I	CCNNNNNNNGG	2 cut(s)	419, 542
Bse1I	ACTGG	4 cut(s)	88, 960, 1043, 1146
Bse21I	CCTNAGG	1 cut(s)	830
Bse8I	GATNNNNATC	2 cut(s)	930, 1130
BseBI	CCWGG	2 cut(s)	403, 938
BseDI	CCNNGG	7 cut(s)	50, 877, 1024, 1154, 1242, 1281, 1324
BseGI	GGATG	5 cut(s)	45, 532, 1142, 1204, 1308
BseJI	GATNNNNATC	2 cut(s)	930, 1130
BseLI	CCNNNNNNNGG	2 cut(s)	419, 542
BseMII	CTCAG	1 cut(s)	821
BseNI	ACTGG	4 cut(s)	88, 960, 1043, 1146
BseSI	GKGCMC	1 cut(s)	514
BseXI	GCAGC	3 cut(s)	415, 1213, 1287
BshFI	GGCC	4 cut(s)	162, 406, 1029, 1166
BshNI	GGYRCC	1 cut(s)	1328
BsiHKAI	GWGCWC	2 cut(s)	18, 514
BsiSI	CCGG	1 cut(s)	1208
BslFI	GGGAC	1 cut(s)	56
BslI	CCNNNNNNNGG	2 cut(s)	419, 542
BsmAI	GTCTC	1 cut(s)	50
BsmFI	GGGAC	1 cut(s)	56
BsnI	GGCC	4 cut(s)	162, 406, 1029, 1166
Bsp1286I	GDGCHC	2 cut(s)	18, 514
Bsp143I	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
Bsp1720I	GCTNAGC	1 cut(s)	12
Bsp19I	CCATGG	1 cut(s)	50
BspACI	CCGC	1 cut(s)	887
BspANI	GGCC	4 cut(s)	162, 406, 1029, 1166
BspCNI	CTCAG	2 cut(s)	4, 822
BspHI	TCATGA	1 cut(s)	1270
BspLI	GGNNCC	4 cut(s)	88, 588, 1059, 1330
BspMI	ACCTGC	2 cut(s)	98, 736
BspOI	GCTAGC	1 cut(s)	1344
BspPI	GGATC	5 cut(s)	117, 939, 1052, 1065, 1145
BspQI	GCTCTTC	1 cut(s)	362
BspT107I	GGYRCC	1 cut(s)	1328
BsrI	ACTGG	4 cut(s)	88, 960, 1043, 1146
BssECI	CCNNGG	7 cut(s)	50, 877, 1024, 1154, 1242, 1281, 1324
BssMI	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
BssT1I	CCWWGG	6 cut(s)	50, 877, 1024, 1154, 1242, 1324
Bst2UI	CCWGG	2 cut(s)	403, 938
Bst4CI	ACNGT	3 cut(s)	220, 516, 1191
Bst6I	CTCTTC	3 cut(s)	362, 765, 954
BstBAI	YACGTR	1 cut(s)	197
BstC8I	GCNNGC	3 cut(s)	1174, 1342, 1353
BstDEI	CTNAG	5 cut(s)	12, 72, 830, 1115, 1356
BstDSI	CCRYGG	1 cut(s)	50
BstEII	GGTNACC	2 cut(s)	1041, 1285
BstF5I	GGATG	5 cut(s)	45, 532, 1142, 1204, 1308
BstH2I	RGCGCY	2 cut(s)	112, 864
BstHHI	GCGC	3 cut(s)	111, 863, 1300
BstKTI	GATC	6 cut(s)	125, 140, 602, 934, 1060, 1153
BstMAI	GTCTC	1 cut(s)	50
BstMBI	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
BstMWI	GCNNNNNNNGC	3 cut(s)	106, 860, 1337
BstNI	CCWGG	2 cut(s)	403, 938
BstNSI	RCATGY	1 cut(s)	696
BstPI	GGTNACC	2 cut(s)	1041, 1285
BstSCI	CCNGG	2 cut(s)	401, 936
BstSFI	CTRYAG	2 cut(s)	133, 591
BstSLI	GKGCMC	1 cut(s)	514
BstV1I	GCAGC	3 cut(s)	415, 1213, 1287
BstX2I	RGATCY	4 cut(s)	122, 599, 931, 1057
BstXI	CCANNNNNNTGG	1 cut(s)	1068
BstYI	RGATCY	4 cut(s)	122, 599, 931, 1057
Bsu36I	CCTNAGG	1 cut(s)	830
BsuI	GTATCC	1 cut(s)	557
BsuRI	GGCC	4 cut(s)	162, 406, 1029, 1166
BtgI	CCRYGG	1 cut(s)	50
BtsCI	GGATG	5 cut(s)	45, 532, 1142, 1204, 1308
BtsIMutI	CAGTG	2 cut(s)	81, 1153
BveI	ACCTGC	2 cut(s)	98, 736
Cac8I	GCNNGC	3 cut(s)	1174, 1342, 1353
CciI	TCATGA	1 cut(s)	1270
CfoI	GCGC	3 cut(s)	111, 863, 1300
Cfr13I	GGNCC	3 cut(s)	4, 161, 874
Csp6I	GTAC	1 cut(s)	739
CviAII	CATG	8 cut(s)	8, 51, 64, 287, 693, 918, 1048, 1271
CviQI	GTAC	1 cut(s)	739
DdeI	CTNAG	5 cut(s)	12, 72, 830, 1115, 1356
DpnI	GATC	6 cut(s)	124, 139, 601, 933, 1059, 1152
DpnII	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
EaeI	YGGCCR	2 cut(s)	1027, 1164
Eam1104I	CTCTTC	3 cut(s)	362, 765, 954
EarI	CTCTTC	3 cut(s)	362, 765, 954
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	6 cut(s)	50, 877, 1024, 1154, 1242, 1324
Eco24I	GRGCYC	1 cut(s)	18
Eco32I	GATATC	2 cut(s)	313, 1183
Eco47I	GGWCC	2 cut(s)	4, 874
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	2 cut(s)	80, 989
Eco81I	CCTNAGG	1 cut(s)	830
Eco91I	GGTNACC	2 cut(s)	1041, 1285
EcoICRI	GAGCTC	1 cut(s)	16
EcoO65I	GGTNACC	2 cut(s)	1041, 1285
EcoRII	CCWGG	2 cut(s)	401, 936
EcoRV	GATATC	2 cut(s)	313, 1183
EcoT14I	CCWWGG	6 cut(s)	50, 877, 1024, 1154, 1242, 1324
EcoT22I	ATGCAT	1 cut(s)	704
EcoT38I	GRGCYC	1 cut(s)	18
ErhI	CCWWGG	6 cut(s)	50, 877, 1024, 1154, 1242, 1324
FaeI	CATG	8 cut(s)	11, 54, 67, 290, 696, 921, 1051, 1274
FalI	AAGNNNNNCTT	2 cut(s)	1008, 1040
FaqI	GGGAC	1 cut(s)	56
FatI	CATG	8 cut(s)	7, 50, 63, 286, 692, 917, 1047, 1270
Fnu4HI	GCNGC	4 cut(s)	429, 887, 1202, 1301
FokI	GGATG	5 cut(s)	32, 519, 1129, 1191, 1295
FriOI	GRGCYC	1 cut(s)	18
Fsp4HI	GCNGC	4 cut(s)	429, 887, 1202, 1301
FspBI	CTAG	5 cut(s)	537, 753, 1155, 1325, 1341
GlaI	GCGC	3 cut(s)	110, 862, 1299
GluI	GCNGC	4 cut(s)	429, 887, 1202, 1301
GsuI	CTGGAG	2 cut(s)	636, 959
HaeII	RGCGCY	2 cut(s)	112, 864
HaeIII	GGCC	4 cut(s)	162, 406, 1029, 1166
HapII	CCGG	1 cut(s)	1208
HhaI	GCGC	3 cut(s)	111, 863, 1300
Hin1II	CATG	8 cut(s)	11, 54, 67, 290, 696, 921, 1051, 1274
Hin6I	GCGC	3 cut(s)	109, 861, 1298
HinP1I	GCGC	3 cut(s)	109, 861, 1298
HinfI	GANTC	4 cut(s)	768, 1111, 1119, 1267
HpaII	CCGG	1 cut(s)	1208
HphI	GGTGA	3 cut(s)	637, 1035, 1279
Hpy166II	GTNNAC	2 cut(s)	512, 874
Hpy188I	TCNGA	5 cut(s)	127, 812, 900, 969, 1110
Hpy8I	GTNNAC	2 cut(s)	512, 874
HpyAV	CCTTC	5 cut(s)	104, 337, 537, 796, 964
HpyCH4III	ACNGT	3 cut(s)	220, 516, 1191
HpyCH4IV	ACGT	2 cut(s)	196, 1249
HpyCH4V	TGCA	8 cut(s)	431, 512, 572, 702, 731, 1051, 1308, 1351
HpyF10VI	GCNNNNNNNGC	3 cut(s)	106, 860, 1337
HpyF3I	CTNAG	5 cut(s)	12, 72, 830, 1115, 1356
HpySE526I	ACGT	2 cut(s)	196, 1249
Hsp92II	CATG	8 cut(s)	11, 54, 67, 290, 696, 921, 1051, 1274
HspAI	GCGC	3 cut(s)	109, 861, 1298
Kzo9I	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
LguI	GCTCTTC	1 cut(s)	362
Lsp1109I	GCAGC	3 cut(s)	415, 1213, 1287
LweI	GCATC	3 cut(s)	711, 1213, 1317
MaeI	CTAG	5 cut(s)	537, 753, 1155, 1325, 1341
MaeII	ACGT	2 cut(s)	196, 1249
MaeIII	GTNAC	3 cut(s)	1041, 1225, 1285
MalI	GATC	6 cut(s)	124, 139, 601, 933, 1059, 1152
MboI	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
MboII	GAAGA	8 cut(s)	224, 379, 532, 594, 752, 971, 1106, 1227
MfeI	CAATTG	2 cut(s)	562, 732
MflI	RGATCY	4 cut(s)	122, 599, 931, 1057
MhlI	GDGCHC	2 cut(s)	18, 514
MlsI	TGGCCA	1 cut(s)	1029
MluCI	AATT	9 cut(s)	240, 349, 448, 562, 614, 645, 732, 901, 986
MluNI	TGGCCA	1 cut(s)	1029
MlyI	GAGTC	2 cut(s)	762, 1128
MnlI	CCTC	9 cut(s)	99, 454, 825, 922, 934, 955, 1031, 1244, 1276
Mox20I	TGGCCA	1 cut(s)	1029
Mph1103I	ATGCAT	1 cut(s)	704
MscI	TGGCCA	1 cut(s)	1029
MseI	TTAA	4 cut(s)	239, 644, 947, 989
MslI	CAYNNNNRTG	3 cut(s)	697, 916, 1275
Msp20I	TGGCCA	1 cut(s)	1029
MspI	CCGG	1 cut(s)	1208
MspR9I	CCNGG	2 cut(s)	403, 938
MunI	CAATTG	2 cut(s)	562, 732
MvaI	CCWGG	2 cut(s)	403, 938
MwoI	GCNNNNNNNGC	3 cut(s)	106, 860, 1337
NcoI	CCATGG	1 cut(s)	50
NdeII	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
NheI	GCTAGC	1 cut(s)	1340
NlaIII	CATG	8 cut(s)	11, 54, 67, 290, 696, 921, 1051, 1274
NlaIV	GGNNCC	4 cut(s)	88, 588, 1059, 1330
NmeAIII	GCCGAG	1 cut(s)	1306
NmuCI	GTSAC	3 cut(s)	1041, 1225, 1285
NsiI	ATGCAT	1 cut(s)	704
NspI	RCATGY	1 cut(s)	696
PagI	TCATGA	1 cut(s)	1270
PciI	ACATGT	1 cut(s)	692
PciSI	GCTCTTC	1 cut(s)	362
PfeI	GAWTC	2 cut(s)	1111, 1267
PfoI	TCCNGGA	1 cut(s)	936
PkrI	GCNGC	4 cut(s)	430, 888, 1203, 1302
PleI	GAGTC	2 cut(s)	762, 1127
PpsI	GAGTC	2 cut(s)	762, 1127
Ppu21I	YACGTR	1 cut(s)	197
PscI	ACATGT	1 cut(s)	692
Psp124BI	GAGCTC	1 cut(s)	18
Psp6I	CCWGG	2 cut(s)	401, 936
PspEI	GGTNACC	2 cut(s)	1041, 1285
PspGI	CCWGG	2 cut(s)	401, 936
PspN4I	GGNNCC	4 cut(s)	88, 588, 1059, 1330
PspPI	GGNCC	3 cut(s)	4, 161, 874
PsuI	RGATCY	4 cut(s)	122, 599, 931, 1057
RsaI	GTAC	1 cut(s)	740
RsaNI	GTAC	1 cut(s)	739
RseI	CAYNNNNRTG	3 cut(s)	697, 916, 1275
SacI	GAGCTC	1 cut(s)	18
SapI	GCTCTTC	1 cut(s)	362
SaqAI	TTAA	4 cut(s)	239, 644, 947, 989
SatI	GCNGC	4 cut(s)	429, 887, 1202, 1301
Sau3AI	GATC	6 cut(s)	122, 137, 599, 931, 1057, 1150
Sau96I	GGNCC	3 cut(s)	4, 161, 874
ScaI	AGTACT	1 cut(s)	740
SchI	GAGTC	2 cut(s)	762, 1128
ScrFI	CCNGG	2 cut(s)	403, 938
SduI	GDGCHC	2 cut(s)	18, 514
SfaNI	GCATC	3 cut(s)	711, 1213, 1317
SfcI	CTRYAG	2 cut(s)	133, 591
SinI	GGWCC	2 cut(s)	4, 874
SmiMI	CAYNNNNRTG	3 cut(s)	697, 916, 1275
SmlI	CTYRAG	2 cut(s)	103, 328
SmoI	CTYRAG	2 cut(s)	103, 328
Sse9I	AATT	9 cut(s)	240, 349, 448, 562, 614, 645, 732, 901, 986
SsiI	CCGC	1 cut(s)	887
SspI	AATATT	2 cut(s)	1065, 1081
SspMI	CTAG	5 cut(s)	537, 753, 1155, 1325, 1341
SstI	GAGCTC	1 cut(s)	18
StyD4I	CCNGG	2 cut(s)	401, 936
StyI	CCWWGG	6 cut(s)	50, 877, 1024, 1154, 1242, 1324
TaaI	ACNGT	3 cut(s)	220, 516, 1191
TaiI	ACGT	2 cut(s)	199, 1252
TaqI	TCGA	1 cut(s)	150
TasI	AATT	9 cut(s)	240, 349, 448, 562, 614, 645, 732, 901, 986
TatI	WGTACW	1 cut(s)	738
TauI	GCSGC	1 cut(s)	889
TfiI	GAWTC	2 cut(s)	1111, 1267
Tru1I	TTAA	4 cut(s)	239, 644, 947, 989
Tru9I	TTAA	4 cut(s)	239, 644, 947, 989
TscAI	CASTG	2 cut(s)	88, 1153
TseFI	GTSAC	3 cut(s)	1041, 1225, 1285
TseI	GCWGC	3 cut(s)	428, 1201, 1300
Tsp45I	GTSAC	3 cut(s)	1041, 1225, 1285
TspDTI	ATGAA	7 cut(s)	172, 261, 346, 702, 738, 1271, 1280
TspRI	CASTG	2 cut(s)	88, 1153
VneI	GTGCAC	1 cut(s)	510
VpaK11BI	GGWCC	2 cut(s)	4, 874
XapI	RAATTY	2 cut(s)	448, 901
XbaI	TCTAGA	1 cut(s)	752
XceI	RCATGY	1 cut(s)	696
XcmI	CCANNNNNNNNNTGG	1 cut(s)	539
XmaJI	CCTAGG	2 cut(s)	1154, 1324
XspI	CTAG	5 cut(s)	537, 753, 1155, 1325, 1341
ZrmI	AGTACT	1 cut(s)	740
Zsp2I	ATGCAT	1 cut(s)	704

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.