MD02G1004900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012205
Physical Location & Seq
Forward (+)
346786 .. 349837
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1004900.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGATATGTTTTCAAAGATTTTCCGTGTTCAATCTTCTCTGTTTGATCATTTTGTTGGATGCGGTTTTATCCCAAGAAAATGCTCAAACAATGAGGGACAAGGCATCCTTGCTTTCTTTCAGGGAAGGGATTGTTTCAGACCCCCACCGCACTTTGGAGGACTGGAATTCTTGGGGTGTTCATGTTTGTAATTGGTCAGGCATCAAGTGTAACAAAGCTAGAGACCGCGTCGTGGAGCTTGATCTTAGTGGAAAGGCACTCGGAGGCACCATTTCCCCGTCTCTCGCTAATCTTTCTTCCTTGCACATTCTTGATTTGTCAAGCAACTTTTTTGAAGGTCGCATCCCACCCGAGTTGGGCTCTCTTTCCCTACTTGAACAACTCAGCTTATCTTCTAATCTTCTCGAAGGAAGAATTGCTGCTGAACTTGGTTTTCTTCGCAAAATAGTATATCTTGATTTGGGAAGCAACCGGCTCACTGGTGGAATCCCAATGCCGCTTTTCTGCAATCATTCTTCGTCCTCTCTGCAGTACATAGACCTCTCTAACAATTCTTTAAGCGGCGAAATTCCTTTGAAAGATGGATGTGAGCTTAAACAACTGAGGTTTCTTCTGTTGTGGTCCAACCGTCTAGTTGGACAGGTTCCTGCAGCCATGTCGAACTCCTCAAAACTTAAATGGATCGATTTGGAGTCTAACATGCTGAGTGGGGAGTTGCCATCGGAGATTATACAAAAACTGCCTAAACTGCAGTACCTCTACTTGTCCTACAATCGCTTTATGAGCCATGACGGTAACACTAACCTTGAACCTTTTTTCTCCTCTTTGGTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGAAATAATCTTGGAGGAGATTTACCTCCTATTATTGGTAATCTTTCTACCAATCTTGTACAGCTCCACTTAGATGCTAATCGCATTTATGGTTCAATTCCTCCTCACATTTCAAATCTTGTCAACCTCACCCTGTTGAATTTGTCTAGTAACCTTCTAAATGGAACCATCCCGCCCAAACTGTGCCGAATGACAATGCTAGAGAGGGTGTTTTTGTCAAACAATTCACTCTCCGGTGAGATTCCATCCGCCTTTGGTGACATTCCCCATCTAGGCCTTCTAGATTTATCGAAAAATAAGCTTTCCGGTTCCATTCCAGATAGTTTTGCAAATCTTTTCCAGTTAAGAAGGCTCCTGCTTTATGAAAACCAACTCTCGGGGACTATACCACCGAGCCTAGGGAAGTGCATCAATTTAGAGATTCTAGACCTTTCTCACAACCAAATTTCTGGAGTGATTCCGAGTGAAGTTGCAGGGCTGAGGAGCTTGAAGTTGTACTTGAACCTGTCTAGCAATCACTTGCATGGATTGTTACCGATGGAGTTGAGCAAAATGGACATGGTGCTAGCAGTTGACCTATCTTCCAACAATCTTTCCGGCACAATCCCATCACAACTTGGGAGCTGCATTGCCCTTGAGTACCTCAACCTTTCTGCGAATTCCTTAGAAGGCTCTCTACCGGATTCAATAGGAAAGTTGCCTTATCTTCAAAGTCTTGATGTATCCTCGAACCAACTAAATGGAGAAATACCAGAATCTCTGCAGGAATCATTAACTCTGAAGAAACTCAACTTCTCTTTCAACAACTTCTCTGGGAATGTATCAAGCAATGGGGCCTTTTCTTCGCTGACTATAGACTCTTTCCTCGGAAATCCTGGTCTCTGTGGATCAATAAAGGGCATGTCACACTGCCGGAAGAAGCGTACTCATCATTTGGCTATTCTGTCAATTATCTTGTCATTACTTATCACCCCCATATTTTGTGTATTCGGGTACCCCCTCATGCACAGGTCGAAATTCCAAAGGCACCAGGCAGTTTTCGATCAAGAGGAATTGGGGGATGATGAAGAAGAAGGGAAGAAAGAACACAAGTACCCGAGAATCTCTTACGACCAGCTCATTGAAGCCACTGGCGGTTTCAGCCCTTCGAGCCTGATTGGTTCAGGCCGGTTTGGGCATGTCTATAAGGGTGTCCTCCAAGACAACTCAGTAATTGCTGTGAAGGTATTGGATTTGAAAACGGATGAAGAGATCTTAGGAAGCTTCAAAAGGGAATGCAGAGTTCTGAAGAGGACTAGGCACAGAAATTTGATCAGAATCATCACGGCGTGTAGTAGGCCGGACTTTAAGGCTCTTGTTCTGCCATTGATGTCAAATGGGAGCCTCGAGAGGCATCTATACTCAAACCATGGACTGAGCGTTCGGTTGAATTTGATTCAGTTGGTAAGCATCTGCAGTGACGTAGCTGAAGCCATGTCCTATTTACATCACCATTCTCCTGTTAGAGTTGTACACTGTGATCTCAAACCGAGCAACATTCTACTAGATGAGGACATGACGGCGTTGGTAACTGATTTTGGAATTGCGAGATTGGTGAAGGGCGATGATGGGAATGTTCCAACGAATGATTCAGCATCCTTCAGTTCAGCAGATGGCTTTTTATGCGGATCCATTGGCTACATTGCTCCTGAGTATGCATTGGGAAAAACCATTTCTACTGAGGGAGACGTGTTCAGCTTCGGGGTCCTTTTGCTAGAAATCGTTACAGGAAGGCGACCAACGGATGTCCTTGCTCACGAAGGTTCAACTCTTCATGAATGGGTGAAAAGGCAGTACCTGCACAGGCTCGATCCAATAGTTCAACAAGCGCTCGATAGGTGCGCTGCTGATTCGATGCCTAAACAGTACAACAAAGTCTGGAGGGATGTTGTTTTGGAACTGATTGAGCTTGGTCTGATGTGCACTCGGTACACTCCTTCATTGAGACCTAATATGCAGGACGTAGCTCACGAAATCGGTCGGTTGAAGGATTACATCTCCAATCCTTCATCGTTTCTGATTGAAGAAATTGATCTTAAAGTTGAAGCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

986

Amino Acids

108.75

Weight (kDa)

6.05

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 3.6e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 200 - 260 1.7e-08 Leucine rich repeat
LRR_8 PF13855 355 - 413 1.4e-07 Leucine rich repeat
LRR_4 PF12799 402 - 438 8.8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 424 - 462 4.7e-06 Leucine rich repeat
LRR_14 PF23598 469 - 544 5.1e-06 Leucine-rich repeat region
LRR_8 PF13855 479 - 534 4.9e-08 Leucine rich repeat
Pkinase PF00069 671 - 958 9.5e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 673 - 957 6.2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2709
Acc65I GGTACC 1 cut(s) 1857
AccB1I GGYRCC 3 cut(s) 266, 1857, 1890
AccII CGCG 1 cut(s) 228
AciI CCGC 9 cut(s) 62, 148, 226, 497, 561, 1037, 1113, 1998, 2529
AclWI GGATC 5 cut(s) 689, 1759, 2526, 2539, 2708
AcsI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
AcuI CTGAAG 4 cut(s) 1664, 2171, 2352, 2488
AdeI CACNNNGTG 1 cut(s) 2193
AfeI AGCGCT 1 cut(s) 2733
AfiI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
AflIII ACRYGT 1 cut(s) 2592
AjiI CACGTC 1 cut(s) 2593
AjnI CCWGG 2 cut(s) 1738, 1893
AjuI GAANNNNNNNTTGG 2 cut(s) 2476, 2508
AloI GAACNNNNNNTCC 2 cut(s) 2268, 2300
Alw21I GWGCWC 1 cut(s) 2828
Alw26I GTCTC 5 cut(s) 216, 285, 1748, 2583, 2842
Alw44I GTGCAC 1 cut(s) 2824
AlwI GGATC 5 cut(s) 689, 1759, 2526, 2539, 2708
AlwNI CAGNNNCTG 1 cut(s) 2701
Ama87I CYCGRG 4 cut(s) 350, 1240, 1960, 2249
Aor51HI AGCGCT 1 cut(s) 2733
AoxI GGCC 4 cut(s) 1138, 1698, 2029, 2201
ApaLI GTGCAC 1 cut(s) 2824
ApeKI GCWGC 4 cut(s) 419, 650, 1488, 2747
ApoI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
ArsI GACNNNNNNTTYG 2 cut(s) 2268, 2300
Asp718I GGTACC 1 cut(s) 1857
AspA2I CCTAGG 1 cut(s) 1261
AspLEI GCGC 2 cut(s) 2734, 2747
AspS9I GGNCC 3 cut(s) 621, 1698, 2608
AsuHPI GGTGA 7 cut(s) 985, 1112, 1133, 1825, 2345, 2470, 2698
AsuNHI GCTAGC 1 cut(s) 1429
AvaI CYCGRG 4 cut(s) 350, 1240, 1960, 2249
AvaII GGWCC 2 cut(s) 621, 2608
AvrII CCTAGG 1 cut(s) 1261
BaeGI GKGCMC 1 cut(s) 2828
BaeI ACNNNNGTAYC 4 cut(s) 737, 770, 1940, 1973
BamHI GGATCC 1 cut(s) 2531
BanI GGYRCC 3 cut(s) 266, 1857, 1890
BanII GRGCYC 1 cut(s) 362
BarI GAAGNNNNNNTAC 2 cut(s) 1524, 1556
Bbv12I GWGCWC 1 cut(s) 2828
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 4 cut(s) 406, 662, 1475, 2734
BccI CCATC 9 cut(s) 575, 727, 1040, 1117, 1140, 1396, 1480, 2465, 2510
BceAI ACGGC 2 cut(s) 2205, 2439
BcgI CGANNNNNNTGC 2 cut(s) 639, 673
BciT130I CCWGG 2 cut(s) 1740, 1895
BciVI GTATCC 1 cut(s) 1597
BclI TGATCA 2 cut(s) 45, 2175
BcoDI GTCTC 5 cut(s) 216, 285, 1748, 2583, 2842
BfmI CTRYAG 6 cut(s) 527, 648, 749, 1625, 1716, 2317
BfoI RGCGCY 1 cut(s) 2735
BfuAI ACCTGC 1 cut(s) 2709
BfuI GTATCC 1 cut(s) 1597
BglII AGATCT 1 cut(s) 2115
BisI GCNGC 6 cut(s) 420, 497, 562, 651, 1489, 2748
BlnI CCTAGG 1 cut(s) 1261
BlsI GCNGC 6 cut(s) 421, 498, 563, 652, 1490, 2749
Bme1390I CCNGG 2 cut(s) 1740, 1895
Bme18I GGWCC 2 cut(s) 621, 2608
BmeT110I CYCGRG 4 cut(s) 350, 1240, 1960, 2249
BmgBI CACGTC 1 cut(s) 2593
BmgT120I GGNCC 3 cut(s) 621, 1698, 2608
BmrFI CCNGG 2 cut(s) 1740, 1895
BmtI GCTAGC 1 cut(s) 1433
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 2 cut(s) 1335, 2803
Bpu10I CCTNAGC 1 cut(s) 1343
BpuEI CTTGAG 1 cut(s) 1520
Bsa29I ATCGAT 1 cut(s) 684
BsaBI GATNNNNATC 2 cut(s) 942, 2180
BsaI GGTCTC 3 cut(s) 216, 1748, 2842
BsaJI CCNNGG 3 cut(s) 1261, 1729, 2272
BsaWI WCCGGW 3 cut(s) 1097, 1169, 1543
Bsc4I CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
Bse118I RCCGGY 2 cut(s) 471, 2031
Bse1I ACTGG 4 cut(s) 167, 484, 1204, 1999
Bse3DI GCAATG 3 cut(s) 1491, 1699, 2544
Bse8I GATNNNNATC 2 cut(s) 942, 2180
BseBI CCWGG 2 cut(s) 1740, 1895
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 1261, 1729, 2272
BseJI GATNNNNATC 2 cut(s) 942, 2180
BseLI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
BseMI GCAATG 3 cut(s) 1491, 1699, 2544
BseMII CTCAG 8 cut(s) 397, 593, 695, 1334, 2085, 2270, 2544, 2574
BseNI ACTGG 4 cut(s) 167, 484, 1204, 1999
BseRI GAGGAG 5 cut(s) 655, 811, 894, 957, 1360
BseSI GKGCMC 1 cut(s) 2828
BseXI GCAGC 4 cut(s) 406, 662, 1475, 2734
BsgI GTGCAG 1 cut(s) 2687
Bsh1236I CGCG 1 cut(s) 228
Bsh1285I CGRYCG 1 cut(s) 2884
BshFI GGCC 4 cut(s) 1140, 1700, 2031, 2203
BshNI GGYRCC 3 cut(s) 266, 1857, 1890
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 2884
BsiHKAI GWGCWC 1 cut(s) 2828
BsiHKCI CYCGRG 4 cut(s) 350, 1240, 1960, 2249
BsiSI CCGG 8 cut(s) 472, 1098, 1170, 1461, 1544, 1777, 2032, 2204
BslFI GGGAC 2 cut(s) 110, 1258
BslI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
BsmAI GTCTC 5 cut(s) 216, 285, 1748, 2583, 2842
BsmBI CGTCTC 2 cut(s) 285, 2583
BsmFI GGGAC 2 cut(s) 110, 1258
BsmI GAATGC 1 cut(s) 2144
BsnI GGCC 4 cut(s) 1140, 1700, 2031, 2203
Bso31I GGTCTC 3 cut(s) 216, 1748, 2842
BsoBI CYCGRG 4 cut(s) 350, 1240, 1960, 2249
Bsp1286I GDGCHC 2 cut(s) 362, 2828
Bsp1407I TGTACA 2 cut(s) 922, 2374
Bsp19I CCATGG 1 cut(s) 2272
BspACI CCGC 9 cut(s) 62, 148, 226, 497, 561, 1037, 1113, 1998, 2529
BspANI GGCC 4 cut(s) 1140, 1700, 2031, 2203
BspCNI CTCAG 8 cut(s) 396, 594, 696, 1335, 2084, 2271, 2545, 2575
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 2677
BspMAI CTGCAG 5 cut(s) 531, 652, 753, 1629, 2321
BspMI ACCTGC 1 cut(s) 2709
BspOI GCTAGC 1 cut(s) 1433
BspPI GGATC 5 cut(s) 689, 1759, 2526, 2539, 2708
BspT107I GGYRCC 3 cut(s) 266, 1857, 1890
BspTNI GGTCTC 3 cut(s) 216, 1748, 2842
BsrDI GCAATG 3 cut(s) 1491, 1699, 2544
BsrFI RCCGGY 2 cut(s) 471, 2031
BsrGI TGTACA 2 cut(s) 922, 2374
BsrI ACTGG 4 cut(s) 167, 484, 1204, 1999
BssAI RCCGGY 2 cut(s) 471, 2031
BssECI CCNNGG 3 cut(s) 1261, 1729, 2272
BssT1I CCWWGG 2 cut(s) 1261, 2272
Bst2UI CCWGG 2 cut(s) 1740, 1895
Bst4CI ACNGT 5 cut(s) 629, 794, 1047, 2381, 2769
Bst6I CTCTTC 3 cut(s) 2106, 2147, 2679
BstAPI GCANNNNNTGC 1 cut(s) 2701
BstAUI TGTACA 2 cut(s) 922, 2374
BstC8I GCNNGC 1 cut(s) 1431
BstDSI CCRYGG 1 cut(s) 2272
BstFNI CGCG 1 cut(s) 228
BstH2I RGCGCY 1 cut(s) 2735
BstHHI GCGC 2 cut(s) 2734, 2747
BstMAI GTCTC 5 cut(s) 216, 285, 1748, 2583, 2842
BstMCI CGRYCG 1 cut(s) 2884
BstMWI GCNNNNNNNGC 6 cut(s) 748, 783, 2004, 2013, 2526, 2701
BstNI CCWGG 2 cut(s) 1740, 1895
BstNSI RCATGY 3 cut(s) 703, 1768, 2045
BstSCI CCNGG 2 cut(s) 1738, 1893
BstSFI CTRYAG 6 cut(s) 527, 648, 749, 1625, 1716, 2317
BstSLI GKGCMC 1 cut(s) 2828
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 4 cut(s) 406, 662, 1475, 2734
BstX2I RGATCY 2 cut(s) 2115, 2531
BstXI CCANNNNNNTGG 2 cut(s) 1313, 1604
BstYI RGATCY 2 cut(s) 2115, 2531
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 1 cut(s) 1597
BsuRI GGCC 4 cut(s) 1140, 1700, 2031, 2203
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 2272
BtgZI GCGATG 1 cut(s) 2481
BtrI CACGTC 1 cut(s) 2593
BtsI GCAGTG 2 cut(s) 1771, 2326
BtsIMutI CAGTG 5 cut(s) 477, 1771, 1992, 2326, 2377
BveI ACCTGC 1 cut(s) 2709
Cac8I GCNNGC 1 cut(s) 1431
CaiI CAGNNNCTG 1 cut(s) 2701
CciI TCATGA 1 cut(s) 2677
CfoI GCGC 2 cut(s) 2734, 2747
Cfr10I RCCGGY 2 cut(s) 471, 2031
Cfr13I GGNCC 3 cut(s) 621, 1698, 2608
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 217
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
DraIII CACNNNGTG 1 cut(s) 2193
Eam1104I CTCTTC 3 cut(s) 2106, 2147, 2679
EarI CTCTTC 3 cut(s) 2106, 2147, 2679
EciI GGCGGA 1 cut(s) 1102
Eco130I CCWWGG 2 cut(s) 1261, 2272
Eco147I AGGCCT 1 cut(s) 1140
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 3 cut(s) 216, 1748, 2842
Eco47I GGWCC 2 cut(s) 621, 2608
Eco47III AGCGCT 1 cut(s) 2733
Eco57I CTGAAG 4 cut(s) 1664, 2171, 2352, 2488
Eco88I CYCGRG 4 cut(s) 350, 1240, 1960, 2249
EcoO109I RGGNCCY 2 cut(s) 1698, 2608
EcoRI GAATTC 2 cut(s) 166, 1522
EcoRII CCWGG 2 cut(s) 1738, 1893
EcoT14I CCWWGG 2 cut(s) 1261, 2272
EcoT22I ATGCAT 1 cut(s) 2563
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 1261, 2272
Esp3I CGTCTC 2 cut(s) 285, 2583
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 1346, 1378
FaqI GGGAC 2 cut(s) 110, 1258
FauI CCCGC 1 cut(s) 1044
FbaI TGATCA 2 cut(s) 45, 2175
Fnu4HI GCNGC 6 cut(s) 420, 497, 562, 651, 1489, 2748
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 6 cut(s) 420, 497, 562, 651, 1489, 2748
GlaI GCGC 2 cut(s) 2733, 2746
GluI GCNGC 6 cut(s) 420, 497, 562, 651, 1489, 2748
GsuI CTGGAG 2 cut(s) 1335, 2803
HaeII RGCGCY 1 cut(s) 2735
HaeIII GGCC 4 cut(s) 1140, 1700, 2031, 2203
HapII CCGG 8 cut(s) 472, 1098, 1170, 1461, 1544, 1777, 2032, 2204
HgaI GACGC 1 cut(s) 217
HhaI GCGC 2 cut(s) 2734, 2747
Hin6I GCGC 2 cut(s) 2732, 2745
HinP1I GCGC 2 cut(s) 2732, 2745
HincII GTYRAC 2 cut(s) 988, 1438
HindII GTYRAC 2 cut(s) 988, 1438
HindIII AAGCTT 2 cut(s) 1163, 2125
HpaII CCGG 8 cut(s) 472, 1098, 1170, 1461, 1544, 1777, 2032, 2204
HphI GGTGA 7 cut(s) 985, 1112, 1133, 1825, 2345, 2470, 2698
Hpy166II GTNNAC 5 cut(s) 988, 1438, 2377, 2826, 2835
Hpy8I GTNNAC 5 cut(s) 988, 1438, 2377, 2826, 2835
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 5 cut(s) 629, 794, 1047, 2381, 2769
HpyCH4IV ACGT 3 cut(s) 2325, 2592, 2865
HpyF10VI GCNNNNNNNGC 6 cut(s) 748, 783, 2004, 2013, 2526, 2701
HpySE526I ACGT 3 cut(s) 2325, 2592, 2865
HspAI GCGC 2 cut(s) 2732, 2745
KpnI GGTACC 1 cut(s) 1861
Ksp22I TGATCA 2 cut(s) 45, 2175
LmnI GCTCC 7 cut(s) 235, 933, 1221, 1347, 1485, 2244, 2554
Lsp1109I GCAGC 4 cut(s) 406, 662, 1475, 2734
MaeII ACGT 3 cut(s) 2325, 2592, 2865
MaeIII GTNAC 9 cut(s) 209, 794, 1013, 1121, 1395, 1767, 2321, 2431, 2626
MflI RGATCY 2 cut(s) 2115, 2531
MhlI GDGCHC 2 cut(s) 362, 2828
MlyI GAGTC 2 cut(s) 701, 1715
MmeI TCCRAC 5 cut(s) 36, 616, 648, 1473, 2507
Mph1103I ATGCAT 1 cut(s) 2563
MseI TTAA 8 cut(s) 557, 594, 675, 831, 1208, 1637, 2211, 2940
MslI CAYNNNNRTG 2 cut(s) 936, 1386
MspI CCGG 8 cut(s) 472, 1098, 1170, 1461, 1544, 1777, 2032, 2204
MspR9I CCNGG 2 cut(s) 1740, 1895
Mva1269I GAATGC 1 cut(s) 2144
MvaI CCWGG 2 cut(s) 1740, 1895
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 6 cut(s) 748, 783, 2004, 2013, 2526, 2701
NcoI CCATGG 1 cut(s) 2272
NheI GCTAGC 1 cut(s) 1429
NmuCI GTSAC 3 cut(s) 1121, 1767, 2321
NsiI ATGCAT 1 cut(s) 2563
NspI RCATGY 3 cut(s) 703, 1768, 2045
PaeR7I CTCGAG 1 cut(s) 2249
PagI TCATGA 1 cut(s) 2677
PceI AGGCCT 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 2871
PctI GAATGC 1 cut(s) 2144
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 6 cut(s) 421, 498, 563, 652, 1490, 2749
PleI GAGTC 2 cut(s) 700, 1715
PpsI GAGTC 2 cut(s) 700, 1715
PpuMI RGGWCCY 1 cut(s) 2608
Psp5II RGGWCCY 1 cut(s) 2608
Psp6I CCWGG 2 cut(s) 1738, 1893
PspGI CCWGG 2 cut(s) 1738, 1893
PspPI GGNCC 3 cut(s) 621, 1698, 2608
PspPPI RGGWCCY 1 cut(s) 2608
PstI CTGCAG 5 cut(s) 531, 652, 753, 1629, 2321
PstNI CAGNNNCTG 1 cut(s) 2701
PsuI RGATCY 2 cut(s) 2115, 2531
PsyI GACNNNGTC 1 cut(s) 227
RseI CAYNNNNRTG 2 cut(s) 936, 1386
SaqAI TTAA 8 cut(s) 557, 594, 675, 831, 1208, 1637, 2211, 2940
SatI GCNGC 6 cut(s) 420, 497, 562, 651, 1489, 2748
Sau96I GGNCC 3 cut(s) 621, 1698, 2608
SchI GAGTC 2 cut(s) 701, 1715
ScrFI CCNGG 2 cut(s) 1740, 1895
SduI GDGCHC 2 cut(s) 362, 2828
SfcI CTRYAG 6 cut(s) 527, 648, 749, 1625, 1716, 2317
Sfr274I CTCGAG 1 cut(s) 2249
SinI GGWCC 2 cut(s) 621, 2608
SlaI CTCGAG 1 cut(s) 2249
SmiMI CAYNNNNRTG 2 cut(s) 936, 1386
SmlI CTYRAG 2 cut(s) 1499, 2249
SmoI CTYRAG 2 cut(s) 1499, 2249
SseBI AGGCCT 1 cut(s) 1140
SsiI CCGC 9 cut(s) 62, 148, 226, 497, 561, 1037, 1113, 1998, 2529
StuI AGGCCT 1 cut(s) 1140
StyD4I CCNGG 2 cut(s) 1738, 1893
StyI CCWWGG 2 cut(s) 1261, 2272
TaaI ACNGT 5 cut(s) 629, 794, 1047, 2381, 2769
TaiI ACGT 3 cut(s) 2328, 2595, 2868
TaqII GACCGA 1 cut(s) 2870
TatI WGTACW 5 cut(s) 531, 922, 1359, 2374, 2769
TauI GCSGC 2 cut(s) 499, 564
Tru1I TTAA 8 cut(s) 557, 594, 675, 831, 1208, 1637, 2211, 2940
Tru9I TTAA 8 cut(s) 557, 594, 675, 831, 1208, 1637, 2211, 2940
TscAI CASTG 5 cut(s) 484, 1778, 1999, 2326, 2384
TseFI GTSAC 3 cut(s) 1121, 1767, 2321
TseI GCWGC 4 cut(s) 419, 650, 1488, 2747
Tsp45I GTSAC 3 cut(s) 1121, 1767, 2321
TspDTI ATGAA 8 cut(s) 170, 1242, 1944, 2124, 2666, 2694, 2832, 2901
TspGWI ACGGA 3 cut(s) 13, 2120, 2660
TspRI CASTG 5 cut(s) 484, 1778, 1999, 2326, 2384
Tth111I GACNNNGTC 1 cut(s) 227
VneI GTGCAC 1 cut(s) 2824
VpaK11BI GGWCC 2 cut(s) 621, 2608
XapI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
XbaI TCTAGA 2 cut(s) 1144, 1288
XceI RCATGY 3 cut(s) 703, 1768, 2045
XhoI CTCGAG 1 cut(s) 2249
XmaJI CCTAGG 1 cut(s) 1261
Zsp2I ATGCAT 1 cut(s) 2563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.