MD15G1148900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012205
Physical Location & Seq
Forward (+)
11014902 .. 11018089
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1148900.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGATCATGATGTGTTTTGGAAGGTCTTCTGTGTTTAAGCTTCTCTGTTTGATCATTTTGTTGGAAGCGGTTTTATGCCAAGAAACTGCTCAAATAATGAAGGACAAGGCATCCTTGCTTTCTTTCAGAGAAGGGATTGTATCGGACCCCCACCGGGCTTTAGAGGACTGGAATTCCTTGGGTGTTCATCTTTGTAATTGGTCAGGCATCAAGTGTAACAAAGCTAGAGACCGCGTCGTGGAGCTTGATCTTAGTGGAAAGGCACTCGGAGGCACCATTTCCCCGTCTCTCGCTAATCTTTCTTCCTTACACATTCTTGATCTATCAAGTAATTTTTTCAAAGGTCGCATTCCAGGCGAGTTAGGCACTCTTTCTCAACTTGGAGAACTCAGCTTATCTTCCAATCTTCTAGAGGGAAGCATCGCTGCTGAACTTGGTTTTCTTCGCAAATTAGTGTATCTCGATTTGGGAAGCAACCGGCTCACTGGTGAAATCCCAATGCCGCTTTTCTGCAATCATTCTTCGTCTTCTCTGCAGTACTTAGACCTCTCTAACAATTCTTTAAGCGGTGAAATTCCTTTGCCAGATGAATGTGAGCTTATACACCTGAGGTTTCTTCTACTGTGGTCCAACCGTCTAGTTGGACTTGTTCCTGCAGCCATTTCAAACTCCTCAAAACTTAAATGGCTCGATTTAGAGTCTAACATGCTGAAAGGGGAGTTGCCATCGGAGATTATACGAAAAATGCCACAACTGCAGTACCTCTACTTGTCCTACAATCGCTTTGTGAGCCATGACGGTAACACTAACCTTGAACCCTTTTTTGCCTCTTTGGTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGAAACAATCTTGGAGGAGATTTACCTCCTATTATTGGTAATCTTTCTACCAATCTTGTACAACTTCACTTAGATACTAATCGTATTTACGGTTCAATTCCCCCTCATATTTCAAATCTTGTCAACCTCACCCTGTTGAATTTGTCTAGTAACCTTCTGAATGGAACCATCCCACCCAAACTGTGCCAAATGACAAAGCTAGAGAGGGTGTTTTTCTCAAACAATTCACTGTCTGGTGAGATTCCATCTGCCTTTGGTGACATTCCCCATCTAGGCCTTCTAGATTTATCCAAAAACAAGCTTTCCGGTTCCATTCCAGATAGTTTTTCAAACCTTTCCCAGTTAAGAAGGCTCTTTCTTTATGAGAACCAACTCACAGGGACCATACCGCCAAGTCTAGGAAAGTGCATCAATTTAGAGATTCTAGACCTTTCTCAGAACCAAATTTCAGGGGTGATTCCTAGCGAAGTTGCAGGGCTGAGGAGCTTGAAGTTGTACTTGAACCTGTCTAGCAATCACTTGCATGGACTGTTACCGATGGAGTTGAGCAAAATGGACATGGTGCTAGCAGTTGACCTATCTTCCAACAATCTTTCCGGCACAATACCATCACAACTTGGGAGCTGCATTGCCCTCGAGTACCTCAACCTTTCGGTGAATTCCTTAGAAGGCCCTCTTCCGGATTCAATAGGAAAGCTACCTTATCTTCAAATACTTGACGTATCCTCAAACCAACTGAATGGACAAATACCAGTGTCTCTGCAGGAATCATTAACTCTGAAGAAACTCAACTTCTCTTTCAACAACTTATCCGGGAATGTATCAAACAATGGGGCCTTTTCTTTGCTGACCATAGACTCATTCCTGGGTAATGCTGGTCTCTGTGGATCAATAAAGGGCATGTCAAACTGCCGGAAGAAGCATACTCATCATTTGGCTATTCTGCCAATTATCCTGTCATTACTTATCACCCCCATATTTTGTGTATTCGGGTACCCCCTCATGCATGGGTCGAAATTCCAAAAACACTTAGCAGTTTTCAATCAAGAGGATTCGGGGGATGATGAAAAAGAAGGGAAGAAAGAACACAAGTGTCCGAAAATCTCATACGAGCAGCTCATTGAAGCCACGGGTGGTTTCAGTGCTTCCAGCCTGATTGGTTCAGGTGGGTTTGGGCATGTCTATAAGGGTGTCCTTCAAGACAACTCAGTAATTGCTGTCAAGGTATTGGATTTGAAAACGGATGGAGATAATTTGGGAACCTTCAAAAGGGAATGCAGAGTTCTGAAGAGGACTAGGCACAGAAATTTGATCAGAATCATCACGGCGTGCAGTAGGCCGGACTTTAAGGCTCTTGTTCTGCCATTGATGTCAAATGGGAGCCTCGAGAGTCACCTATACCCGAGCCATGGGCTGAAGGATCAGTTGAATTTGATTCAGTTGGTGAGCATCTGCAGTGATGTAGCTGAAGGAGTGGCCTATTTACACCATCACTCTCCTATTAGAGTTGTGCACTGTGATCTCAAACCGAGCAACATTCTACTAGATGAGGACATGACGGCGTTGGTAACTGATTTTGGAATTGCGAGATTGGTGAAGGGCGGCGATGAGAAGGGTCCAACGAATGATTCAGCATCCTTCAGTTCAGCAGATGGCTTGTTATGCGGATCCATTGGCTACATTGCTCCTGAGTATGCGATGGGAAAAACCATTTCTACTGAGGGAGACGTGTTCAGTTACGGGGTCCTTTTGCTAGAAATCGTTACAGGAAGGCGACCAACGGATGTCCTTGTTCACGAAGGTTCAACCCTTCACGAATGGGTGAAAAGTCAGTACCCGCGCAGGCTCGATCCAATAGTTCAACAAGCGCTCGATAGGTGTGCTGCGGATTTGATGCCTAATCAGTACAACAAAGTATGGAGGGATGTTGTTTTGGAATTGATAGAGCTTGGTCTGATCTGCACTCAGTACAATCCTTCAATGAGACCAAATATGCAGGATATAGCTCACGAAATCGGTCGGTTGAAGGATTACATCTCCAGTCCTGCATCGTTTCTGATTGATGAAATTGATCTTAAAGTTGAAGCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

988

Amino Acids

108.13

Weight (kDa)

5.97

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 73 6e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 177 - 263 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 202 - 262 4.6e-10 Leucine rich repeat
LRR_8 PF13855 356 - 415 5.7e-08 Leucine rich repeat
LRR_4 PF12799 404 - 440 1.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 481 - 536 7e-07 Leucine rich repeat
Pkinase PF00069 672 - 960 1.5e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 674 - 961 3.8e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1863
AccB1I GGYRCC 2 cut(s) 272, 1863
AccII CGCG 2 cut(s) 234, 2710
AccIII TCCGGA 1 cut(s) 1549
AciI CCGC 9 cut(s) 68, 232, 503, 567, 1259, 2472, 2535, 2708, 2756
AclWI GGATC 5 cut(s) 1765, 2298, 2532, 2545, 2714
AcsI RAATTY 8 cut(s) 172, 573, 1009, 1314, 1528, 1886, 2176, 2299
AcuI CTGAAG 5 cut(s) 1670, 2177, 2306, 2358, 2494
AdeI CACNNNGTG 1 cut(s) 2199
AfaI GTAC 9 cut(s) 539, 761, 930, 1367, 1511, 1865, 2705, 2777, 2840
AfeI AGCGCT 1 cut(s) 2739
AfiI CCNNNNNNNGG 8 cut(s) 154, 238, 905, 1142, 1268, 1549, 2139, 2279
AflIII ACRYGT 1 cut(s) 2598
AjiI CACGTC 1 cut(s) 2599
AjnI CCWGG 2 cut(s) 352, 1734
AjuI GAANNNNNNNTTGG 2 cut(s) 2482, 2514
Alw21I GWGCWC 1 cut(s) 2385
Alw26I GTCTC 6 cut(s) 222, 291, 1632, 1754, 2589, 2848
Alw44I GTGCAC 1 cut(s) 2381
AlwI GGATC 5 cut(s) 1765, 2298, 2532, 2545, 2714
Ama87I CYCGRG 3 cut(s) 1505, 2255, 2272
Aor13HI TCCGGA 1 cut(s) 1549
Aor51HI AGCGCT 1 cut(s) 2739
AoxI GGCC 5 cut(s) 1144, 1540, 1704, 2207, 2346
ApaLI GTGCAC 1 cut(s) 2381
ApeKI GCWGC 5 cut(s) 425, 656, 1494, 1984, 2753
ApoI RAATTY 8 cut(s) 172, 573, 1009, 1314, 1528, 1886, 2176, 2299
Asp700I GAANNNNTTC 1 cut(s) 25
Asp718I GGTACC 1 cut(s) 1863
AspLEI GCGC 2 cut(s) 2712, 2740
AspS9I GGNCC 7 cut(s) 145, 627, 1251, 1541, 1704, 2486, 2614
AsuC2I CCSGG 2 cut(s) 155, 1684
AsuNHI GCTAGC 1 cut(s) 1435
AvaI CYCGRG 3 cut(s) 1505, 2255, 2272
AvaII GGWCC 5 cut(s) 145, 627, 1251, 2486, 2614
AxyI CCTNAGG 1 cut(s) 608
BaeGI GKGCMC 1 cut(s) 2385
BaeI ACNNNNGTAYC 2 cut(s) 743, 776
BamHI GGATCC 1 cut(s) 2537
BanI GGYRCC 2 cut(s) 272, 1863
BbsI GAAGAC 2 cut(s) 18, 519
Bbv12I GWGCWC 1 cut(s) 2385
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 5 cut(s) 412, 668, 1481, 1996, 2740
BceAI ACGGC 2 cut(s) 2211, 2445
BcgI CGANNNNNNTGC 4 cut(s) 1486, 1520, 2732, 2766
BciT130I CCWGG 2 cut(s) 354, 1736
BciVI GTATCC 1 cut(s) 1603
BclI TGATCA 3 cut(s) 3, 51, 2181
BcnI CCSGG 2 cut(s) 155, 1684
BcoDI GTCTC 6 cut(s) 222, 291, 1632, 1754, 2589, 2848
BfmI CTRYAG 5 cut(s) 533, 654, 755, 1631, 2323
BfoI RGCGCY 1 cut(s) 2741
BfuI GTATCC 1 cut(s) 1603
BisI GCNGC 7 cut(s) 426, 503, 657, 1495, 1985, 2473, 2754
BlsI GCNGC 7 cut(s) 427, 504, 658, 1496, 1986, 2474, 2755
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 4 cut(s) 155, 354, 1684, 1736
Bme18I GGWCC 5 cut(s) 145, 627, 1251, 2486, 2614
BmeT110I CYCGRG 3 cut(s) 1505, 2255, 2272
BmgBI CACGTC 1 cut(s) 2599
BmgT120I GGNCC 7 cut(s) 145, 627, 1251, 1541, 1704, 2486, 2614
BmrFI CCNGG 4 cut(s) 155, 354, 1684, 1736
BmrI ACTGGG 1 cut(s) 1204
BmsI GCATC 8 cut(s) 119, 216, 429, 1287, 2328, 2513, 2754, 2927
BmtI GCTAGC 1 cut(s) 1439
BmuI ACTGGG 1 cut(s) 1204
BpiI GAAGAC 2 cut(s) 18, 519
BpmI CTGGAG 1 cut(s) 2893
Bpu10I CCTNAGC 1 cut(s) 1349
BpuMI CCSGG 2 cut(s) 155, 1684
BsaBI GATNNNNATC 2 cut(s) 948, 2186
BsaI GGTCTC 3 cut(s) 222, 1754, 2848
BsaJI CCNNGG 4 cut(s) 177, 1735, 1998, 2278
BsaWI WCCGGW 2 cut(s) 1175, 1549
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 238, 905, 1142, 1268, 1549, 2139, 2279
Bse118I RCCGGY 1 cut(s) 477
Bse1I ACTGG 5 cut(s) 173, 490, 1210, 1622, 2910
Bse21I CCTNAGG 1 cut(s) 608
Bse3DI GCAATG 2 cut(s) 1497, 2550
Bse8I GATNNNNATC 2 cut(s) 948, 2186
BseAI TCCGGA 1 cut(s) 1549
BseBI CCWGG 2 cut(s) 354, 1736
BseDI CCNNGG 4 cut(s) 177, 1735, 1998, 2278
BseGI GGATG 7 cut(s) 110, 1038, 1936, 2119, 2504, 2659, 2800
BseJI GATNNNNATC 2 cut(s) 948, 2186
BseLI CCNNNNNNNGG 8 cut(s) 154, 238, 905, 1142, 1268, 1549, 2139, 2279
BseMI GCAATG 2 cut(s) 1497, 2550
BseMII CTCAG 8 cut(s) 403, 599, 1319, 1340, 2091, 2550, 2580, 2849
BseNI ACTGG 5 cut(s) 173, 490, 1210, 1622, 2910
BseRI GAGGAG 3 cut(s) 661, 900, 1366
BseSI GKGCMC 1 cut(s) 2385
BseXI GCAGC 5 cut(s) 412, 668, 1481, 1996, 2740
BsgI GTGCAG 2 cut(s) 2221, 2815
Bsh1236I CGCG 2 cut(s) 234, 2710
Bsh1285I CGRYCG 1 cut(s) 2890
BshFI GGCC 5 cut(s) 1146, 1542, 1706, 2209, 2348
BshNI GGYRCC 2 cut(s) 272, 1863
BsiEI CGRYCG 1 cut(s) 2890
BsiHKAI GWGCWC 1 cut(s) 2385
BsiHKCI CYCGRG 3 cut(s) 1505, 2255, 2272
BsiSI CCGG 8 cut(s) 154, 478, 1176, 1467, 1550, 1683, 1783, 2210
BslFI GGGAC 1 cut(s) 1264
BslI CCNNNNNNNGG 8 cut(s) 154, 238, 905, 1142, 1268, 1549, 2139, 2279
BsmAI GTCTC 6 cut(s) 222, 291, 1632, 1754, 2589, 2848
BsmBI CGTCTC 2 cut(s) 291, 2589
BsmFI GGGAC 1 cut(s) 1264
BsmI GAATGC 2 cut(s) 348, 2150
BsnI GGCC 5 cut(s) 1146, 1542, 1706, 2209, 2348
Bso31I GGTCTC 3 cut(s) 222, 1754, 2848
BsoBI CYCGRG 3 cut(s) 1505, 2255, 2272
Bsp1286I GDGCHC 1 cut(s) 2385
Bsp13I TCCGGA 1 cut(s) 1549
Bsp1407I TGTACA 1 cut(s) 928
Bsp19I CCATGG 1 cut(s) 2278
BspACI CCGC 9 cut(s) 68, 232, 503, 567, 1259, 2472, 2535, 2708, 2756
BspANI GGCC 5 cut(s) 1146, 1542, 1706, 2209, 2348
BspCNI CTCAG 8 cut(s) 402, 600, 1318, 1341, 2090, 2551, 2581, 2848
BspEI TCCGGA 1 cut(s) 1549
BspFNI CGCG 2 cut(s) 234, 2710
BspHI TCATGA 1 cut(s) 6
BspMAI CTGCAG 5 cut(s) 537, 658, 759, 1635, 2327
BspOI GCTAGC 1 cut(s) 1439
BspPI GGATC 5 cut(s) 1765, 2298, 2532, 2545, 2714
BspT107I GGYRCC 2 cut(s) 272, 1863
BspTNI GGTCTC 3 cut(s) 222, 1754, 2848
BsrDI GCAATG 2 cut(s) 1497, 2550
BsrFI RCCGGY 1 cut(s) 477
BsrGI TGTACA 1 cut(s) 928
BsrI ACTGG 5 cut(s) 173, 490, 1210, 1622, 2910
BssAI RCCGGY 1 cut(s) 477
BssECI CCNNGG 4 cut(s) 177, 1735, 1998, 2278
BssT1I CCWWGG 2 cut(s) 177, 2278
Bst2UI CCWGG 2 cut(s) 354, 1736
Bst4CI ACNGT 8 cut(s) 624, 635, 800, 962, 1053, 1101, 1401, 2387
Bst6I CTCTTC 2 cut(s) 1551, 2153
BstAUI TGTACA 1 cut(s) 928
BstC8I GCNNGC 3 cut(s) 1437, 2200, 2714
BstDSI CCRYGG 2 cut(s) 1998, 2278
BstENI CCTNNNNNAGG 1 cut(s) 2137
BstF5I GGATG 7 cut(s) 110, 1038, 1936, 2119, 2504, 2659, 2800
BstFNI CGCG 2 cut(s) 234, 2710
BstH2I RGCGCY 1 cut(s) 2741
BstHHI GCGC 2 cut(s) 2712, 2740
BstMAI GTCTC 6 cut(s) 222, 291, 1632, 1754, 2589, 2848
BstMCI CGRYCG 1 cut(s) 2890
BstMWI GCNNNNNNNGC 5 cut(s) 354, 363, 754, 789, 2532
BstNI CCWGG 2 cut(s) 354, 1736
BstNSI RCATGY 3 cut(s) 709, 1774, 2051
BstSCI CCNGG 4 cut(s) 153, 352, 1682, 1734
BstSFI CTRYAG 5 cut(s) 533, 654, 755, 1631, 2323
BstSLI GKGCMC 1 cut(s) 2385
BstUI CGCG 2 cut(s) 234, 2710
BstV1I GCAGC 5 cut(s) 412, 668, 1481, 1996, 2740
BstV2I GAAGAC 2 cut(s) 18, 519
BstX2I RGATCY 1 cut(s) 2537
BstXI CCANNNNNNTGG 1 cut(s) 1610
BstYI RGATCY 1 cut(s) 2537
Bsu36I CCTNAGG 1 cut(s) 608
BsuI GTATCC 1 cut(s) 1603
BsuRI GGCC 5 cut(s) 1146, 1542, 1706, 2209, 2348
BtgI CCRYGG 2 cut(s) 1998, 2278
BtgZI GCGATG 3 cut(s) 406, 2490, 2582
BtrI CACGTC 1 cut(s) 2599
BtsCI GGATG 7 cut(s) 110, 1038, 1936, 2119, 2504, 2659, 2800
BtsI GCAGTG 1 cut(s) 2332
BtsIMutI CAGTG 6 cut(s) 483, 1097, 1629, 2017, 2332, 2383
Cac8I GCNNGC 3 cut(s) 1437, 2200, 2714
CciI TCATGA 1 cut(s) 6
CfoI GCGC 2 cut(s) 2712, 2740
Cfr10I RCCGGY 1 cut(s) 477
Cfr13I GGNCC 7 cut(s) 145, 627, 1251, 1541, 1704, 2486, 2614
CseI GACGC 1 cut(s) 223
Csp6I GTAC 9 cut(s) 538, 760, 929, 1366, 1510, 1864, 2704, 2776, 2839
CviQI GTAC 9 cut(s) 538, 760, 929, 1366, 1510, 1864, 2704, 2776, 2839
DraIII CACNNNGTG 1 cut(s) 2199
Eam1104I CTCTTC 2 cut(s) 1551, 2153
EarI CTCTTC 2 cut(s) 1551, 2153
Eco130I CCWWGG 2 cut(s) 177, 2278
Eco147I AGGCCT 1 cut(s) 1146
Eco31I GGTCTC 3 cut(s) 222, 1754, 2848
Eco47I GGWCC 5 cut(s) 145, 627, 1251, 2486, 2614
Eco47III AGCGCT 1 cut(s) 2739
Eco57I CTGAAG 5 cut(s) 1670, 2177, 2306, 2358, 2494
Eco81I CCTNAGG 1 cut(s) 608
Eco88I CYCGRG 3 cut(s) 1505, 2255, 2272
EcoNI CCTNNNNNAGG 1 cut(s) 2137
EcoO109I RGGNCCY 3 cut(s) 1541, 1704, 2614
EcoRI GAATTC 2 cut(s) 172, 1528
EcoRII CCWGG 2 cut(s) 352, 1734
EcoT14I CCWWGG 2 cut(s) 177, 2278
EcoT22I ATGCAT 1 cut(s) 1878
ErhI CCWWGG 2 cut(s) 177, 2278
Esp3I CGTCTC 2 cut(s) 291, 2589
FalI AAGNNNNNCTT 6 cut(s) 98, 130, 1352, 1384, 1530, 1562
FaqI GGGAC 1 cut(s) 1264
FauI CCCGC 1 cut(s) 2715
FbaI TGATCA 3 cut(s) 3, 51, 2181
Fnu4HI GCNGC 7 cut(s) 426, 503, 657, 1495, 1985, 2473, 2754
FokI GGATG 7 cut(s) 97, 1025, 1943, 2126, 2491, 2666, 2807
Fsp4HI GCNGC 7 cut(s) 426, 503, 657, 1495, 1985, 2473, 2754
GlaI GCGC 2 cut(s) 2711, 2739
GluI GCNGC 7 cut(s) 426, 503, 657, 1495, 1985, 2473, 2754
GsuI CTGGAG 1 cut(s) 2893
HaeII RGCGCY 1 cut(s) 2741
HaeIII GGCC 5 cut(s) 1146, 1542, 1706, 2209, 2348
HapII CCGG 8 cut(s) 154, 478, 1176, 1467, 1550, 1683, 1783, 2210
HgaI GACGC 1 cut(s) 223
HhaI GCGC 2 cut(s) 2712, 2740
Hin6I GCGC 2 cut(s) 2710, 2738
HinP1I GCGC 2 cut(s) 2710, 2738
HincII GTYRAC 2 cut(s) 994, 1444
HindII GTYRAC 2 cut(s) 994, 1444
HindIII AAGCTT 2 cut(s) 38, 1169
HpaII CCGG 8 cut(s) 154, 478, 1176, 1467, 1550, 1683, 1783, 2210
Hpy166II GTNNAC 4 cut(s) 994, 1444, 2383, 2665
Hpy8I GTNNAC 4 cut(s) 994, 1444, 2383, 2665
Hpy99I CGWCG 1 cut(s) 239
HpyCH4III ACNGT 8 cut(s) 624, 635, 800, 962, 1053, 1101, 1401, 2387
HpyCH4IV ACGT 2 cut(s) 1590, 2598
HpyF10VI GCNNNNNNNGC 5 cut(s) 354, 363, 754, 789, 2532
HpySE526I ACGT 2 cut(s) 1590, 2598
HspAI GCGC 2 cut(s) 2710, 2738
Kpn2I TCCGGA 1 cut(s) 1549
KpnI GGTACC 1 cut(s) 1867
Ksp22I TGATCA 3 cut(s) 3, 51, 2181
LmnI GCTCC 5 cut(s) 241, 1353, 1491, 2250, 2560
Lsp1109I GCAGC 5 cut(s) 412, 668, 1481, 1996, 2740
LweI GCATC 8 cut(s) 119, 216, 429, 1287, 2328, 2513, 2754, 2927
MaeII ACGT 2 cut(s) 1590, 2598
MaeIII GTNAC 9 cut(s) 215, 800, 1019, 1127, 1401, 2261, 2437, 2606, 2632
MflI RGATCY 1 cut(s) 2537
MhlI GDGCHC 1 cut(s) 2385
MlyI GAGTC 3 cut(s) 707, 1721, 2269
MmeI TCCRAC 5 cut(s) 42, 622, 654, 1479, 2513
Mph1103I ATGCAT 1 cut(s) 1878
MroI TCCGGA 1 cut(s) 1549
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 8 cut(s) 36, 563, 681, 837, 1214, 1643, 2217, 2946
MslI CAYNNNNRTG 1 cut(s) 1392
MspI CCGG 8 cut(s) 154, 478, 1176, 1467, 1550, 1683, 1783, 2210
MspR9I CCNGG 4 cut(s) 155, 354, 1684, 1736
Mva1269I GAATGC 2 cut(s) 348, 2150
MvaI CCWGG 2 cut(s) 354, 1736
MvnI CGCG 2 cut(s) 234, 2710
MwoI GCNNNNNNNGC 5 cut(s) 354, 363, 754, 789, 2532
NciI CCSGG 2 cut(s) 155, 1684
NcoI CCATGG 1 cut(s) 2278
NheI GCTAGC 1 cut(s) 1435
NmuCI GTSAC 2 cut(s) 1127, 2261
NsiI ATGCAT 1 cut(s) 1878
NspI RCATGY 3 cut(s) 709, 1774, 2051
PaeR7I CTCGAG 2 cut(s) 1505, 2255
PagI TCATGA 1 cut(s) 6
PceI AGGCCT 1 cut(s) 1146
PctI GAATGC 2 cut(s) 348, 2150
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 9 cut(s) 1111, 1291, 1327, 1553, 1637, 1922, 2187, 2305, 2498
PflFI GACNNNGTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 1682
PkrI GCNGC 7 cut(s) 427, 504, 658, 1496, 1986, 2474, 2755
PleI GAGTC 3 cut(s) 706, 1721, 2268
PpsI GAGTC 3 cut(s) 706, 1721, 2268
PpuMI RGGWCCY 1 cut(s) 2614
Psp5II RGGWCCY 1 cut(s) 2614
Psp6I CCWGG 2 cut(s) 352, 1734
PspGI CCWGG 2 cut(s) 352, 1734
PspPI GGNCC 7 cut(s) 145, 627, 1251, 1541, 1704, 2486, 2614
PspPPI RGGWCCY 1 cut(s) 2614
PspXI VCTCGAGB 1 cut(s) 1505
PsrI GAACNNNNNNTAC 2 cut(s) 946, 978
PstI CTGCAG 5 cut(s) 537, 658, 759, 1635, 2327
PsuI RGATCY 1 cut(s) 2537
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 9 cut(s) 539, 761, 930, 1367, 1511, 1865, 2705, 2777, 2840
RsaNI GTAC 9 cut(s) 538, 760, 929, 1366, 1510, 1864, 2704, 2776, 2839
RseI CAYNNNNRTG 1 cut(s) 1392
SaqAI TTAA 8 cut(s) 36, 563, 681, 837, 1214, 1643, 2217, 2946
SatI GCNGC 7 cut(s) 426, 503, 657, 1495, 1985, 2473, 2754
Sau96I GGNCC 7 cut(s) 145, 627, 1251, 1541, 1704, 2486, 2614
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 3 cut(s) 707, 1721, 2269
ScrFI CCNGG 4 cut(s) 155, 354, 1684, 1736
SduI GDGCHC 1 cut(s) 2385
SfaNI GCATC 8 cut(s) 119, 216, 429, 1287, 2328, 2513, 2754, 2927
SfcI CTRYAG 5 cut(s) 533, 654, 755, 1631, 2323
Sfr274I CTCGAG 2 cut(s) 1505, 2255
SinI GGWCC 5 cut(s) 145, 627, 1251, 2486, 2614
SlaI CTCGAG 2 cut(s) 1505, 2255
SmiMI CAYNNNNRTG 1 cut(s) 1392
SmlI CTYRAG 2 cut(s) 1505, 2255
SmoI CTYRAG 2 cut(s) 1505, 2255
SseBI AGGCCT 1 cut(s) 1146
SsiI CCGC 9 cut(s) 68, 232, 503, 567, 1259, 2472, 2535, 2708, 2756
StuI AGGCCT 1 cut(s) 1146
StyD4I CCNGG 4 cut(s) 153, 352, 1682, 1734
StyI CCWWGG 2 cut(s) 177, 2278
TaaI ACNGT 8 cut(s) 624, 635, 800, 962, 1053, 1101, 1401, 2387
TaiI ACGT 2 cut(s) 1593, 2601
TaqI TCGA 7 cut(s) 462, 690, 1506, 1883, 2256, 2718, 2742
TaqII GACCGA 1 cut(s) 2876
TatI WGTACW 5 cut(s) 537, 928, 1365, 2775, 2838
TauI GCSGC 2 cut(s) 505, 2475
TfiI GAWTC 9 cut(s) 1111, 1291, 1327, 1553, 1637, 1922, 2187, 2305, 2498
Tru1I TTAA 8 cut(s) 36, 563, 681, 837, 1214, 1643, 2217, 2946
Tru9I TTAA 8 cut(s) 36, 563, 681, 837, 1214, 1643, 2217, 2946
TscAI CASTG 6 cut(s) 490, 1104, 1629, 2017, 2332, 2390
TseFI GTSAC 2 cut(s) 1127, 2261
TseI GCWGC 5 cut(s) 425, 656, 1494, 1984, 2753
Tsp45I GTSAC 2 cut(s) 1127, 2261
TspDTI ATGAA 5 cut(s) 113, 176, 603, 1950, 2949
TspGWI ACGGA 2 cut(s) 2126, 2666
TspRI CASTG 6 cut(s) 490, 1104, 1629, 2017, 2332, 2390
Tth111I GACNNNGTC 1 cut(s) 233
VneI GTGCAC 1 cut(s) 2381
VpaK11BI GGWCC 5 cut(s) 145, 627, 1251, 2486, 2614
XagI CCTNNNNNAGG 1 cut(s) 2137
XapI RAATTY 8 cut(s) 172, 573, 1009, 1314, 1528, 1886, 2176, 2299
XbaI TCTAGA 3 cut(s) 409, 1150, 1294
XceI RCATGY 3 cut(s) 709, 1774, 2051
XhoI CTCGAG 2 cut(s) 1505, 2255
XmnI GAANNNNTTC 1 cut(s) 25
ZrmI AGTACT 1 cut(s) 539
Zsp2I ATGCAT 1 cut(s) 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.