pycom02g00360
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012205
Physical Location & Seq
Forward (+)
222391 .. 225442
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00360.1

Sequence Viewer

Length: 2949 bp
ATGATATGTTTTCAAAGATTTTCCATGTTTAATCTTCTCTGTTTGAACATTTTGTTGGAAGCGGTTTTATCCCAAGAAAATGCTCAAACAATGAGGGACAAGGCATCCTTGCTTTCTTTCAGGGAAGGGATTGTTTCAGATCCCCACCGTGCTTTGGAGGACTGGAATTCTTTTGGTGTTCATGTTTGTAATTGGTCAGGCATCAAGTGTAACAAAGCTAGAGACCACGTCGTGGAGCTTGATCTTAGTAGAAAGGCACTCGGAGGCACCATTTTCCCGTCTCTCGCTAATCTTTCTTCCTTACACATTCTTGATTTGTCAAGCAACTTTTTCGAAGGTCGCATCCCACCCGAGTTAGGCTCTCTTTCTCTACTTGAACAACTCAGCTTATCTTCTAATCTTCTCGAAGGAAGAATTGCTGCTGAACTTGGTTTTCTTCGCAAAATAGTATATCTTGATTTGGGAAGCAACCGGCTCACTGGTGAAATCCCAATGCCGCTTTTCTGCAATCATTCTTCGTCCTCTCTGCAGTACATAGACCTCTCCAACAATTCTTTAAGCGGCGAAATTCCTTTGAAAGATGGATGTGAGCTTAAACAACTGAGGTTTCTTCTGTTGTGGTCCAACCGTCTAGTTGGACAGGTTCCTGCGGCCATGTCGAACTCCTCAAAACTTGAATGGATTGATTTGGAGTCTAACATGCTGAGTGGGGAGTTGCCATCGGAGATTATACAGAAACTGCCAAAACTGCAGTACCTCTACTTGTCCTACAATCGCTTTATGAGTCATGACGGTAACACTAACCTTGAACCTTTTTTCTCCTCTTTGGTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGAAATAATCTTGGAGGAGATTTACCTCCTATTATTGGTAATCTTTCTACCAATCTTGTACAGCTCCACTTAGATGCTAATCGGATTTATGGTTCAATTCCTCCTCACATTTCAAATCTTGTCAACCTCACCCTGTTGAATTTGTCTAGTAACCTTCTAAATGGAACCATCCCGTCCAAACTGTGCCGAATGACAATGCTAGAGAGGGTGTTTTTGTCAAACAATTCACTCTCTGGTGAGATTCCATCCGCCTTTGGTGACATTCCCCATTTAGGCCTTCTAGATTTATCGAAAAATAAGCTTTCCGGTTCCATTCCAGATAGTTTTGCAAATCTTTTCCAGTTAAGAAGGCTCTTGCTTGATGAAAACCAACTCTCGGGGACTATACCACCGAGCCTAGGTAAGTGCATCAATTTAGAGATTCTAGACCTTTCTCACAACCAAATTTCTGGAGTGATTCCGAGCGAAGTTGCAGGGCTGAGGAGCTTGAAGTTGTACTTGAACCTGTCTAACAATCACTTGCATGGACTGTTACCGATGGAGTTGAGCAAAATGGACATGGTGCTAGCAGTTGACCTATCTTCCAACAATCTTTCCGGCACAATCCCATCACAACTTGGGAGCTGCATTGCCCTCGAGTACCTCGACCTTTCTGCGAATTCCTTAGAAGGCTCTCTACCGGATTCAATAGGAAAGCTGCCTTATCTTCAAAAACTTGATGTATCCTCAAACCAACTAAATGGAGAAATACCAGAGTCTCTGCAGGAATCATTAACTCTGAAGAAACTCAACTTCTCTTTCAACAATTTCTCTGGGAATGTATCAAGCAATGGGGCCTTTTCTTCGCTGACTATAGACTCTTTCCTCGGAAATGCTGGTCTCTGTGGATCAATAGAAGGCATGTCAAACTGCCGGAAGAAGCGTACTCATCATTTGGCTATTCTGTCAATTATCTTGTCATTACTTATCACCCCCATATTTTGTGTATTCGGGTACCCCCTCATGCACAGGTCGAAATTCCAAAGGCACCAGGCAGTTTTCGATCAAGTGGATTTGGGGGATGATGAAGAAGAAGGGAAGAAAGAACACAAGTACCCAAGAATCTCTTACGAGCAGCTCATTGAAGCCACTGGCGGTTTCAGCCCTTCTAGCCTGATTGGTTCAGGCCGGTTTGGGCATGTCTATAAGGGTGTCCTCCAAGACAACTCAGTAATTGCTGTGAAGGTATTGGATTTGAAAACGGATGAAGAGATCTTAGGAAGCTTCAAAAGGGAATGCAGAGTTCTGAAGAGGACTAGGCACAGAAATTTGATCAGAATTATCACGGTGTGTAGTAGGCCGGACTTTAAGGCTCTTGTTCTGCCATTGATGTCAAATGGGAGCCTCGAGAGGCATCTATACTCAAACCATGGACTGAGCGTTCGGTTGAATTTGATTCAGTTGGTGAGCATCTGCAGTGATGTAGCTGAAGGCGTGTCCTATTTGCACCACCATTCTCCTGTTAGAGTTGTGCACTGTGATCTCAAACCGAGCAACATTCTACTAGATGAGGACATGACGGCGTTGATAACTGATTTTGGAATTGCGAGATTGGGGAAAGGCGATGATATGAATGTTCCAACGAATGATTCAGCATCCTTCAGTTCAGCAGATGGCTTGTTATGTGGATCCATTGGCTACATTGCTCCTGTGGGAAAAACCATTTCTACTGAGGGAGACGTGTTCAGCTTCGGGGTCCTTTTGCTAGAAATCGTTATAGGAAGGCGACCAACGGATGTCCTTGCTCACGAAGGTTCAACTCTTCATGAATGGGTGAAAAGGCAGTACCCGCACAGGCTCGATCCAATAGTTCAACAAGCGCTCGATAGGTGCGCTGCGGATTCGATGCCTAAACAGGACAACAAAGTCTGGAGGGATGTTGTTTTTGAACTGATTGAGCTTGGTCTGATGTGCACTCAGTACACTCCTTCATTGAGACCTAATATGCAGGATGTAGCTTACGAAATCGGTCGGTTGAAGGATTACATCTCCAATCCTTCATCGTTTCTGATTGAAGAAATTGATCTTAAAGTTGAAGCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

983

Amino Acids

108.47

Weight (kDa)

5.76

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 3.2e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 200 - 260 2.3e-08 Leucine rich repeat
LRR_8 PF13855 355 - 413 2.6e-08 Leucine rich repeat
LRR_8 PF13855 424 - 462 4.3e-06 Leucine rich repeat
LRR_8 PF13855 479 - 534 4.2e-08 Leucine rich repeat
Pkinase PF00069 671 - 953 4.4e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 673 - 953 9.7e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2768
Acc65I GGTACC 1 cut(s) 1857
AccB1I GGYRCC 3 cut(s) 266, 1857, 1890
AccB7I CCANNNNNTGG 1 cut(s) 232
AciI CCGC 8 cut(s) 62, 497, 561, 650, 1113, 1998, 2693, 2741
AclWI GGATC 5 cut(s) 134, 1759, 2526, 2539, 2699
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
AcuI CTGAAG 4 cut(s) 1664, 2171, 2352, 2488
AdeI CACNNNGTG 2 cut(s) 232, 2193
AfeI AGCGCT 1 cut(s) 2724
AfiI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
AflIII ACRYGT 1 cut(s) 2583
AjiI CACGTC 2 cut(s) 229, 2584
AjnI CCWGG 1 cut(s) 1893
AjuI GAANNNNNNNTTGG 4 cut(s) 38, 70, 2476, 2508
AloI GAACNNNNNNTCC 4 cut(s) 940, 972, 2268, 2300
Alw21I GWGCWC 2 cut(s) 2379, 2819
Alw26I GTCTC 6 cut(s) 216, 285, 1626, 1748, 2574, 2833
Alw44I GTGCAC 2 cut(s) 2375, 2815
AlwI GGATC 5 cut(s) 134, 1759, 2526, 2539, 2699
AlwNI CAGNNNCTG 1 cut(s) 739
Ama87I CYCGRG 4 cut(s) 350, 1240, 1499, 2249
Aor51HI AGCGCT 1 cut(s) 2724
AoxI GGCC 5 cut(s) 651, 1138, 1698, 2029, 2201
ApaLI GTGCAC 2 cut(s) 2375, 2815
ApeKI GCWGC 5 cut(s) 419, 1488, 1561, 1978, 2738
ApoI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
ArsI GACNNNNNNTTYG 2 cut(s) 2268, 2300
Asp718I GGTACC 1 cut(s) 1857
AspA2I CCTAGG 1 cut(s) 1261
AspLEI GCGC 2 cut(s) 2725, 2738
AspS9I GGNCC 3 cut(s) 621, 1698, 2599
AsuHPI GGTGA 7 cut(s) 494, 985, 1112, 1133, 1825, 2320, 2689
AsuII TTCGAA 1 cut(s) 333
AsuNHI GCTAGC 1 cut(s) 1429
AvaI CYCGRG 4 cut(s) 350, 1240, 1499, 2249
AvaII GGWCC 2 cut(s) 621, 2599
AvrII CCTAGG 1 cut(s) 1261
BaeGI GKGCMC 2 cut(s) 2379, 2819
BaeI ACNNNNGTAYC 4 cut(s) 737, 770, 1940, 1973
BamHI GGATCC 1 cut(s) 2531
BanI GGYRCC 3 cut(s) 266, 1857, 1890
BarI GAAGNNNNNNTAC 2 cut(s) 1524, 1556
Bbv12I GWGCWC 2 cut(s) 2379, 2819
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 5 cut(s) 406, 1475, 1548, 1990, 2725
BccI CCATC 7 cut(s) 575, 727, 1040, 1117, 1396, 1480, 2510
BceAI ACGGC 1 cut(s) 2439
BcgI CGANNNNNNTGC 6 cut(s) 313, 347, 1480, 1499, 1514, 1533
BciT130I CCWGG 1 cut(s) 1895
BciVI GTATCC 1 cut(s) 1597
BclI TGATCA 1 cut(s) 2175
BcoDI GTCTC 6 cut(s) 216, 285, 1626, 1748, 2574, 2833
BfmI CTRYAG 5 cut(s) 527, 749, 1625, 1716, 2317
BfoI RGCGCY 1 cut(s) 2726
BfuI GTATCC 1 cut(s) 1597
BglII AGATCT 1 cut(s) 2115
BisI GCNGC 8 cut(s) 420, 497, 562, 651, 1489, 1562, 1979, 2739
BlnI CCTAGG 1 cut(s) 1261
BlsI GCNGC 8 cut(s) 421, 498, 563, 652, 1490, 1563, 1980, 2740
Bme1390I CCNGG 1 cut(s) 1895
Bme18I GGWCC 2 cut(s) 621, 2599
BmeT110I CYCGRG 4 cut(s) 350, 1240, 1499, 2249
BmgBI CACGTC 2 cut(s) 229, 2584
BmgT120I GGNCC 3 cut(s) 621, 1698, 2599
BmrFI CCNGG 1 cut(s) 1895
BmsI GCATC 9 cut(s) 113, 210, 351, 928, 1281, 2266, 2322, 2507, 2739
BmtI GCTAGC 1 cut(s) 1433
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 2 cut(s) 1335, 2794
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu14I TTCGAA 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 942
BsaI GGTCTC 3 cut(s) 216, 1748, 2833
BsaJI CCNNGG 3 cut(s) 1261, 1729, 2272
BsaWI WCCGGW 2 cut(s) 1169, 1543
Bsc4I CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
Bse118I RCCGGY 2 cut(s) 471, 2031
Bse1I ACTGG 4 cut(s) 167, 484, 1204, 1999
Bse3DI GCAATG 3 cut(s) 1491, 1699, 2544
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 1 cut(s) 1895
BseDI CCNNGG 3 cut(s) 1261, 1729, 2272
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
BseMI GCAATG 3 cut(s) 1491, 1699, 2544
BseMII CTCAG 8 cut(s) 397, 593, 695, 1334, 2085, 2270, 2565, 2834
BseNI ACTGG 4 cut(s) 167, 484, 1204, 1999
BseRI GAGGAG 5 cut(s) 655, 811, 894, 957, 1360
BseSI GKGCMC 2 cut(s) 2379, 2819
BseXI GCAGC 5 cut(s) 406, 1475, 1548, 1990, 2725
Bsh1285I CGRYCG 1 cut(s) 2875
BshFI GGCC 5 cut(s) 653, 1140, 1700, 2031, 2203
BshNI GGYRCC 3 cut(s) 266, 1857, 1890
BsiEI CGRYCG 1 cut(s) 2875
BsiHKAI GWGCWC 2 cut(s) 2379, 2819
BsiHKCI CYCGRG 4 cut(s) 350, 1240, 1499, 2249
BsiSI CCGG 7 cut(s) 472, 1170, 1461, 1544, 1777, 2032, 2204
BslFI GGGAC 2 cut(s) 110, 1258
BslI CCNNNNNNNGG 7 cut(s) 154, 232, 356, 899, 1136, 1240, 1262
BsmAI GTCTC 6 cut(s) 216, 285, 1626, 1748, 2574, 2833
BsmBI CGTCTC 2 cut(s) 285, 2574
BsmFI GGGAC 2 cut(s) 110, 1258
BsmI GAATGC 1 cut(s) 2144
BsnI GGCC 5 cut(s) 653, 1140, 1700, 2031, 2203
Bso31I GGTCTC 3 cut(s) 216, 1748, 2833
BsoBI CYCGRG 4 cut(s) 350, 1240, 1499, 2249
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 2379, 2819
Bsp1407I TGTACA 1 cut(s) 922
Bsp19I CCATGG 1 cut(s) 2272
BspACI CCGC 8 cut(s) 62, 497, 561, 650, 1113, 1998, 2693, 2741
BspANI GGCC 5 cut(s) 653, 1140, 1700, 2031, 2203
BspCNI CTCAG 8 cut(s) 396, 594, 696, 1335, 2084, 2271, 2566, 2833
BspHI TCATGA 2 cut(s) 787, 2668
BspMAI CTGCAG 4 cut(s) 531, 753, 1629, 2321
BspOI GCTAGC 1 cut(s) 1433
BspPI GGATC 5 cut(s) 134, 1759, 2526, 2539, 2699
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 3 cut(s) 266, 1857, 1890
BspTNI GGTCTC 3 cut(s) 216, 1748, 2833
BsrDI GCAATG 3 cut(s) 1491, 1699, 2544
BsrFI RCCGGY 2 cut(s) 471, 2031
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 4 cut(s) 167, 484, 1204, 1999
BssAI RCCGGY 2 cut(s) 471, 2031
BssECI CCNNGG 3 cut(s) 1261, 1729, 2272
BssT1I CCWWGG 2 cut(s) 1261, 2272
Bst2UI CCWGG 1 cut(s) 1895
Bst4CI ACNGT 7 cut(s) 149, 629, 794, 1047, 1395, 2191, 2381
Bst6I CTCTTC 3 cut(s) 2106, 2147, 2670
BstAUI TGTACA 1 cut(s) 922
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 1431
BstDSI CCRYGG 1 cut(s) 2272
BstH2I RGCGCY 1 cut(s) 2726
BstHHI GCGC 2 cut(s) 2725, 2738
BstMAI GTCTC 6 cut(s) 216, 285, 1626, 1748, 2574, 2833
BstMCI CGRYCG 1 cut(s) 2875
BstMWI GCNNNNNNNGC 4 cut(s) 748, 2004, 2013, 2692
BstNI CCWGG 1 cut(s) 1895
BstNSI RCATGY 3 cut(s) 703, 1768, 2045
BstSCI CCNGG 1 cut(s) 1893
BstSFI CTRYAG 5 cut(s) 527, 749, 1625, 1716, 2317
BstSLI GKGCMC 2 cut(s) 2379, 2819
BstV1I GCAGC 5 cut(s) 406, 1475, 1548, 1990, 2725
BstX2I RGATCY 3 cut(s) 139, 2115, 2531
BstXI CCANNNNNNTGG 2 cut(s) 1313, 1604
BstYI RGATCY 3 cut(s) 139, 2115, 2531
BsuI GTATCC 1 cut(s) 1597
BsuRI GGCC 5 cut(s) 653, 1140, 1700, 2031, 2203
BtgI CCRYGG 1 cut(s) 2272
BtgZI GCGATG 1 cut(s) 2481
BtrI CACGTC 2 cut(s) 229, 2584
BtsI GCAGTG 1 cut(s) 2326
BtsIMutI CAGTG 4 cut(s) 477, 1992, 2326, 2377
Cac8I GCNNGC 1 cut(s) 1431
CaiI CAGNNNCTG 1 cut(s) 739
CciI TCATGA 2 cut(s) 787, 2668
CfoI GCGC 2 cut(s) 2725, 2738
Cfr10I RCCGGY 2 cut(s) 471, 2031
Cfr13I GGNCC 3 cut(s) 621, 1698, 2599
DraIII CACNNNGTG 2 cut(s) 232, 2193
DrdI GACNNNNNNGTC 1 cut(s) 2768
DseDI GACNNNNNNGTC 1 cut(s) 2768
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 3 cut(s) 2106, 2147, 2670
EarI CTCTTC 3 cut(s) 2106, 2147, 2670
EciI GGCGGA 1 cut(s) 1102
Eco130I CCWWGG 2 cut(s) 1261, 2272
Eco147I AGGCCT 1 cut(s) 1140
Eco31I GGTCTC 3 cut(s) 216, 1748, 2833
Eco47I GGWCC 2 cut(s) 621, 2599
Eco47III AGCGCT 1 cut(s) 2724
Eco57I CTGAAG 4 cut(s) 1664, 2171, 2352, 2488
Eco88I CYCGRG 4 cut(s) 350, 1240, 1499, 2249
EcoO109I RGGNCCY 2 cut(s) 1698, 2599
EcoRI GAATTC 2 cut(s) 166, 1522
EcoRII CCWGG 1 cut(s) 1893
EcoT14I CCWWGG 2 cut(s) 1261, 2272
ErhI CCWWGG 2 cut(s) 1261, 2272
Esp3I CGTCTC 2 cut(s) 285, 2574
FalI AAGNNNNNCTT 6 cut(s) 92, 124, 1346, 1378, 1954, 1986
FaqI GGGAC 2 cut(s) 110, 1258
FauI CCCGC 1 cut(s) 2700
FbaI TGATCA 1 cut(s) 2175
Fnu4HI GCNGC 8 cut(s) 420, 497, 562, 651, 1489, 1562, 1979, 2739
Fsp4HI GCNGC 8 cut(s) 420, 497, 562, 651, 1489, 1562, 1979, 2739
GlaI GCGC 2 cut(s) 2724, 2737
GluI GCNGC 8 cut(s) 420, 497, 562, 651, 1489, 1562, 1979, 2739
GsuI CTGGAG 2 cut(s) 1335, 2794
HaeII RGCGCY 1 cut(s) 2726
HaeIII GGCC 5 cut(s) 653, 1140, 1700, 2031, 2203
HapII CCGG 7 cut(s) 472, 1170, 1461, 1544, 1777, 2032, 2204
HhaI GCGC 2 cut(s) 2725, 2738
Hin6I GCGC 2 cut(s) 2723, 2736
HinP1I GCGC 2 cut(s) 2723, 2736
HincII GTYRAC 2 cut(s) 988, 1438
HindII GTYRAC 2 cut(s) 988, 1438
HindIII AAGCTT 2 cut(s) 1163, 2125
HpaII CCGG 7 cut(s) 472, 1170, 1461, 1544, 1777, 2032, 2204
HphI GGTGA 7 cut(s) 494, 985, 1112, 1133, 1825, 2320, 2689
Hpy166II GTNNAC 5 cut(s) 988, 1438, 2377, 2817, 2826
Hpy8I GTNNAC 5 cut(s) 988, 1438, 2377, 2817, 2826
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 7 cut(s) 149, 629, 794, 1047, 1395, 2191, 2381
HpyCH4IV ACGT 2 cut(s) 228, 2583
HpyF10VI GCNNNNNNNGC 4 cut(s) 748, 2004, 2013, 2692
HpySE526I ACGT 2 cut(s) 228, 2583
HspAI GCGC 2 cut(s) 2723, 2736
KpnI GGTACC 1 cut(s) 1861
Ksp22I TGATCA 1 cut(s) 2175
LmnI GCTCC 6 cut(s) 235, 933, 1347, 1485, 2244, 2554
Lsp1109I GCAGC 5 cut(s) 406, 1475, 1548, 1990, 2725
LweI GCATC 9 cut(s) 113, 210, 351, 928, 1281, 2266, 2322, 2507, 2739
MaeII ACGT 2 cut(s) 228, 2583
MaeIII GTNAC 5 cut(s) 209, 794, 1013, 1121, 1395
MflI RGATCY 3 cut(s) 139, 2115, 2531
MhlI GDGCHC 2 cut(s) 2379, 2819
MlyI GAGTC 4 cut(s) 701, 793, 1628, 1715
MmeI TCCRAC 6 cut(s) 36, 570, 616, 648, 1473, 2507
MseI TTAA 8 cut(s) 30, 557, 594, 831, 1208, 1637, 2211, 2931
MslI CAYNNNNRTG 2 cut(s) 936, 1386
MspI CCGG 7 cut(s) 472, 1170, 1461, 1544, 1777, 2032, 2204
MspR9I CCNGG 1 cut(s) 1895
Mva1269I GAATGC 1 cut(s) 2144
MvaI CCWGG 1 cut(s) 1895
MwoI GCNNNNNNNGC 4 cut(s) 748, 2004, 2013, 2692
NcoI CCATGG 1 cut(s) 2272
NheI GCTAGC 1 cut(s) 1429
NmuCI GTSAC 1 cut(s) 1121
NspI RCATGY 3 cut(s) 703, 1768, 2045
NspV TTCGAA 1 cut(s) 333
PaeR7I CTCGAG 2 cut(s) 1499, 2249
PagI TCATGA 2 cut(s) 787, 2668
PceI AGGCCT 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 1506
PctI GAATGC 1 cut(s) 2144
PfeI GAWTC 9 cut(s) 1105, 1285, 1321, 1547, 1631, 1965, 2299, 2492, 2744
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 8 cut(s) 421, 498, 563, 652, 1490, 1563, 1980, 2740
PleI GAGTC 4 cut(s) 700, 792, 1627, 1715
PpsI GAGTC 4 cut(s) 700, 792, 1627, 1715
PpuMI RGGWCCY 1 cut(s) 2599
Psp5II RGGWCCY 1 cut(s) 2599
Psp6I CCWGG 1 cut(s) 1893
PspGI CCWGG 1 cut(s) 1893
PspPI GGNCC 3 cut(s) 621, 1698, 2599
PspPPI RGGWCCY 1 cut(s) 2599
PspXI VCTCGAGB 1 cut(s) 1499
PstI CTGCAG 4 cut(s) 531, 753, 1629, 2321
PstNI CAGNNNCTG 1 cut(s) 739
PsuI RGATCY 3 cut(s) 139, 2115, 2531
PsyI GACNNNGTC 1 cut(s) 227
RseI CAYNNNNRTG 2 cut(s) 936, 1386
SaqAI TTAA 8 cut(s) 30, 557, 594, 831, 1208, 1637, 2211, 2931
SatI GCNGC 8 cut(s) 420, 497, 562, 651, 1489, 1562, 1979, 2739
Sau96I GGNCC 3 cut(s) 621, 1698, 2599
SchI GAGTC 4 cut(s) 701, 793, 1628, 1715
ScrFI CCNGG 1 cut(s) 1895
SduI GDGCHC 2 cut(s) 2379, 2819
SfaNI GCATC 9 cut(s) 113, 210, 351, 928, 1281, 2266, 2322, 2507, 2739
SfcI CTRYAG 5 cut(s) 527, 749, 1625, 1716, 2317
Sfr274I CTCGAG 2 cut(s) 1499, 2249
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 2 cut(s) 621, 2599
SlaI CTCGAG 2 cut(s) 1499, 2249
SmiMI CAYNNNNRTG 2 cut(s) 936, 1386
SmlI CTYRAG 2 cut(s) 1499, 2249
SmoI CTYRAG 2 cut(s) 1499, 2249
SseBI AGGCCT 1 cut(s) 1140
SsiI CCGC 8 cut(s) 62, 497, 561, 650, 1113, 1998, 2693, 2741
StuI AGGCCT 1 cut(s) 1140
StyD4I CCNGG 1 cut(s) 1893
StyI CCWWGG 2 cut(s) 1261, 2272
TaaI ACNGT 7 cut(s) 149, 629, 794, 1047, 1395, 2191, 2381
TaiI ACGT 2 cut(s) 231, 2586
TaqII GACCGA 1 cut(s) 2861
TatI WGTACW 4 cut(s) 531, 922, 1359, 2823
TauI GCSGC 3 cut(s) 499, 564, 653
TfiI GAWTC 9 cut(s) 1105, 1285, 1321, 1547, 1631, 1965, 2299, 2492, 2744
Tru1I TTAA 8 cut(s) 30, 557, 594, 831, 1208, 1637, 2211, 2931
Tru9I TTAA 8 cut(s) 30, 557, 594, 831, 1208, 1637, 2211, 2931
TscAI CASTG 4 cut(s) 484, 1999, 2326, 2384
TseFI GTSAC 1 cut(s) 1121
TseI GCWGC 5 cut(s) 419, 1488, 1561, 1978, 2738
Tsp45I GTSAC 1 cut(s) 1121
TspDTI ATGAA 9 cut(s) 170, 1242, 1944, 2124, 2489, 2657, 2685, 2823, 2892
TspGWI ACGGA 2 cut(s) 2120, 2651
TspRI CASTG 4 cut(s) 484, 1999, 2326, 2384
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 232
VneI GTGCAC 2 cut(s) 2375, 2815
VpaK11BI GGWCC 2 cut(s) 621, 2599
XapI RAATTY 8 cut(s) 166, 567, 1003, 1308, 1522, 1880, 2170, 2293
XbaI TCTAGA 2 cut(s) 1144, 1288
XceI RCATGY 3 cut(s) 703, 1768, 2045
XhoI CTCGAG 2 cut(s) 1499, 2249
XmaJI CCTAGG 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.