Rroxscaffold_2G00155540
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012205
Physical Location & Seq
Reverse (-)
91950988 .. 91954025
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155540.1

Sequence Viewer

Length: 2958 bp
ATGTTCAATTTTCTCTGTTTGATCATCATTTTCTTGGCTGCTGCGGTGTTTTCGAGCCAAGAAAATGTTCATCAAGCACTGAGAGACAAGGCATCGTTGGTTTCATTCATGTCTGGGATTGTTTCAGACCCTCACCATGCTCTAGAGGACTGGAATTCTAATTTGGACGATGTTCATTTTTGTAATTGGACTGGCATCAGATGCAACAATGCCGGAGACCAAGTTGTGGAGCTTGATCTTAGTGGAAAGTCCTTAAGAGGCACTATTTCACCAGCTCTCTCTAATCTTTCTTCCTTAACCATTCTTGATTTGTCAAGCAACTTCTTTGAAGGTCACATTCCAAGAGAGTTAGGCTCTCTTTCTCGGCTCACACAACTCAGCTTGTCTTCAAATCTTCTTGAAGGAAACATTGCAGCTGAGCTTGGTTTCCTTCACAGATTGGTGTATCTTAGTTTGGGAAGCAACCGGCTGGAGGGAGAAATTCCAATGCCGCTTTTCTGCAATCACTCTTCTAATTCTCTGCAGTACATAGACTTGTCTAACAATTCTATAAGCGGCAAAATTCCTTTAATACATGGATGTGAGCTGAAAAAACTGAGGTTTCTTCTACTTTGGTCCAATCATCTAGTTGGACAGGTTCCTGCAGCACTTTCAAACTCCTCGAAACTAGAATGGCTCGACTTAGAATCCAACATGCTAAGTGGGGAGCTCCCATCGGAGATTGTAGCCAGAATGCCAAAGTTGCAGTACCTCTACTTGTCCTACAATGACTTTGTGAGCCATGACGGTAACACTAACCTTGAACCTTTTTTAACCTCTTTACTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGGAATAATCTTGGGGGAGAGATACCGCCTATTATTGGTGATCTTTCGACCAATCTTGTACAAGTTCATTTAGATGGTAATCTTCTTTATGGTTCAATCCCTCCTCACATTTCAAACCTTGTCAACCTCACCCTCTTGAACTTGTCTAGTAACCATTTGAATGGAACCATCCCATCCAAACTATGCCTAATGAGACAGCTGGAACGGGTATATCTCTCGAACAATTCACTCTCTGGTGAAATTCCATCTGAACTGGGTGGCATTCCCCATCTAGGCCTTCTAGATTTATCAAGAAACAAGTTTTCTGGTTCAATCCCAGATAGTTTCGAAAACCTTTCCCAGCTACGAAGGCTATTGCTCTACGAGAATCAACTCTCGGGGACAATACCACCAAGTCTAGGAAAGTGCATCAACTTAGAGATTCTAGACCTTTCTCACAATCAAATGACCGGGGTGATTCCTAGTGAAGTTGCAGGGTTAAGGAGCTTGAAGTTATACTTGAACTTGTCAAGTAATTACTTCAATGGAGAGGTACCAATGGAGCTAAGCAAAATGGACATGGTGCTAGCAATAGATCTGTCTTCCAATAATCTTTCTGGCACAATCCCATCACAACTTGGGAGCTGCATTGCCCTTGAGTCCCTCAACATTTCATCAAATTCCTTACAAGGCCCTGTCCCGGTTTCAATAGGAAAGTTGCCTTTTCTACAGAAACTTGATGTATCTTCAAACCAGTTGGCTGGAGAAATACCAGAATCTCTGCAGCAATCCTTAACTCTCAAGCAACTTAACTTCTCTTTCAACAACTTCTCTGGGAATGTATCAAACAAAGGCGCCTTTTCTTTGCTGAGCTTAGACTCTTTCCTCGGAAATGCTGGCCTCTGTGGCTCAATAAAAGGCATGCCAAGCTGCAAAAAGAAAAAATCTCATCATTTGGCTATACTCTCAGTCCTGCTGTCATTAATCATCACTCCCATTTTTTGCATAGTTGGTTACCCCCTCATGCACAGGTCAAAAATCCGAAGGCACCTGGGAATTTTCGATGACAGGAAATTGCGAGATGATGATGAAGAATTAGGAAAAGAAGAGCATAAGTACCCGAGAATCTCATATGAGCAGCTCATTGAAGCCACTGGTGGTTTCAGTGCTTCAAGCCTGATTGGTTCAGGACGATTTGGGCGCGTCTACAAGGGTATCCTTCATGATAACACAGAAATAGCAGTCAAAGTTTTGGATTTGAAGACAGAAGAAGAAATTTCAGGAAGCTTTAAAAGGGAATGCCAAGTCCTGAAGAGAACCAGGCACAGAAATTTGATTAGAATCATCACAACTTGCAGTAGACCGGACTTTAAGGCTCTTGTTCTGCCATTGATGTCGAATGGGAGCCTTGAGAGGCATCTTTACTCGTCGTCATCAAGCCATTGTGGATTGAATCTGATTCAGCTGGTGAGCATCTGCAGTGATGTAGCTGAAGGAGTTGCCTATTTGCACCATCATTCTCCTGTTAGAATTGTACACTGTGATCTTAAGCCGAGCAACATTCTACTAGATGATGACATGACAGCTTTGGTTACTGATTTTGGAATTGCAAGACTAGTTAAAGGTGAAGACGAGAGTATTCTAATGAATGACTCAACATCCTTCAGCTCAGCAGATGGCTTGTTATGTGGATCCATTGGCTATATAGCTCCTGAGTATGGAATGGGAAAATGTGCTTCGACTCAGGGAGATGTATTCAGCTTTGGGGTGCTTCTGTTAGAAATTGTTACAGGTAGGCGGCCAACGGATGTCGATTTTCACGAAGGTTCAAGCTTACAGGAATGGATCAAAACCCAGTACCCCCAGAGGCTCGAGCCTATAGTTCAACAAGCAGTAGAAAGGTGTGCCCCAGCTTCAATGCCAAAGCAATATAACAAAATATGGGGGGATGTAGTTCTGGAGTTGATTGAGATTGGTCTTATGTGCACACAGCACAATCCTTCATTGCGCCCAAATATGCAGGATGTTGCTTATGAAATGAGCCAGGTGAAGGAGTACATCTCCAATCCTTCGTCAGCGTTGTTGATTGAAGAAGTGGACACTAAGGTTGATTCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

985

Amino Acids

108.26

Weight (kDa)

5.61

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 69 1.2e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 198 - 258 1.2e-07 Leucine rich repeat
LRR_8 PF13855 351 - 411 1e-06 Leucine rich repeat
LRR_14 PF23598 417 - 569 1.4e-06 Leucine-rich repeat region
LRR_8 PF13855 477 - 532 5.1e-07 Leucine rich repeat
Pkinase PF00069 669 - 954 2.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 671 - 956 3.8e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1390
AccB1I GGYRCC 3 cut(s) 1390, 1691, 1884
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 2 cut(s) 2043, 2197
AccII CGCG 1 cut(s) 2040
AciI CCGC 5 cut(s) 44, 491, 555, 884, 2635
AclWI GGATC 3 cut(s) 2523, 2536, 2690
AcoI YGGCCR 1 cut(s) 2636
AcsI RAATTY 8 cut(s) 154, 480, 561, 1098, 1516, 1893, 2112, 2167
AcuI CTGAAG 3 cut(s) 2168, 2349, 2485
AcyI GRCGYC 1 cut(s) 1692
AfaI GTAC 8 cut(s) 527, 749, 918, 1392, 1955, 2373, 2696, 2894
AfiI CCNNNNNNNGG 8 cut(s) 226, 472, 893, 1130, 1256, 1537, 2555, 2887
AflII CTTAAG 2 cut(s) 253, 2384
AhlI ACTAGT 1 cut(s) 2452
AjnI CCWGG 3 cut(s) 1887, 2156, 2880
AjuI GAANNNNNNNTTGG 4 cut(s) 51, 83, 146, 178
Alw21I GWGCWC 2 cut(s) 711, 2825
Alw26I GTCTC 3 cut(s) 78, 210, 1045
Alw44I GTGCAC 1 cut(s) 2821
AlwI GGATC 3 cut(s) 2523, 2536, 2690
Ama87I CYCGRG 3 cut(s) 1234, 1957, 2708
AoxI GGCC 4 cut(s) 1132, 1528, 1735, 2636
ApaLI GTGCAC 1 cut(s) 2821
ApeKI GCWGC 8 cut(s) 38, 41, 413, 644, 1482, 1621, 1767, 1975
ApoI RAATTY 8 cut(s) 154, 480, 561, 1098, 1516, 1893, 2112, 2167
AseI ATTAAT 1 cut(s) 1820
Asp700I GAANNNNTTC 1 cut(s) 66
Asp718I GGTACC 1 cut(s) 1390
AspLEI GCGC 3 cut(s) 1694, 2040, 2847
AspS9I GGNCC 2 cut(s) 615, 1529
AsuC2I CCSGG 2 cut(s) 1309, 1538
AsuHPI GGTGA 9 cut(s) 125, 261, 908, 979, 1106, 1324, 2317, 2474, 2896
AsuII TTCGAA 1 cut(s) 1185
AsuNHI GCTAGC 1 cut(s) 1423
AvaI CYCGRG 3 cut(s) 1234, 1957, 2708
AvaII GGWCC 1 cut(s) 615
BaeGI GKGCMC 2 cut(s) 2746, 2825
BaeI ACNNNNGTAYC 2 cut(s) 2035, 2068
BamHI GGATCC 1 cut(s) 2528
BanI GGYRCC 3 cut(s) 1390, 1691, 1884
BanII GRGCYC 1 cut(s) 711
BbsI GAAGAC 4 cut(s) 378, 1431, 2105, 2472
Bbv12I GWGCWC 2 cut(s) 711, 2825
BbvI GCAGC 8 cut(s) 25, 28, 425, 656, 1469, 1633, 1754, 1987
BccI CCATC 9 cut(s) 721, 926, 1034, 1039, 1111, 1134, 1474, 2358, 2507
BciT130I CCWGG 3 cut(s) 1889, 2158, 2882
BciVI GTATCC 1 cut(s) 2063
BclI TGATCA 1 cut(s) 21
BcnI CCSGG 2 cut(s) 1309, 1538
BcoDI GTCTC 3 cut(s) 78, 210, 1045
BcuI ACTAGT 1 cut(s) 2452
BfmI CTRYAG 6 cut(s) 521, 642, 1565, 1619, 2314, 2715
BfoI RGCGCY 1 cut(s) 1695
BfrI CTTAAG 2 cut(s) 253, 2384
BfuI GTATCC 1 cut(s) 2063
BglI GCCNNNNNGGC 1 cut(s) 1743
BglII AGATCT 1 cut(s) 1432
BlpI GCTNAGC 4 cut(s) 417, 1403, 1706, 2506
Bme1390I CCNGG 5 cut(s) 1309, 1538, 1889, 2158, 2882
Bme18I GGWCC 1 cut(s) 615
BmeT110I CYCGRG 3 cut(s) 1234, 1957, 2708
BmgT120I GGNCC 2 cut(s) 615, 1529
BmiI GGNNCC 7 cut(s) 639, 1024, 1392, 1693, 1886, 2243, 2530
BmrFI CCNGG 5 cut(s) 1309, 1538, 1889, 2158, 2882
BmrI ACTGGG 2 cut(s) 1121, 2686
BmsI GCATC 6 cut(s) 101, 191, 204, 1275, 2263, 2319
BmtI GCTAGC 1 cut(s) 1427
BmuI ACTGGG 2 cut(s) 1121, 2686
BpiI GAAGAC 4 cut(s) 378, 1431, 2105, 2472
BplI GAGNNNNNCTC 4 cut(s) 338, 370, 2882, 2914
BpmI CTGGAG 3 cut(s) 491, 1620, 2816
Bpu1102I GCTNAGC 4 cut(s) 417, 1403, 1706, 2506
Bpu14I TTCGAA 1 cut(s) 1185
BpuEI CTTGAG 3 cut(s) 1514, 1622, 2267
BpuMI CCSGG 2 cut(s) 1309, 1538
BsaBI GATNNNNATC 2 cut(s) 936, 2177
BsaHI GRCGYC 1 cut(s) 1692
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 1308, 1723, 1888
BsaWI WCCGGW 1 cut(s) 2200
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 8 cut(s) 226, 472, 893, 1130, 1256, 1537, 2555, 2887
Bse118I RCCGGY 1 cut(s) 465
Bse1I ACTGG 6 cut(s) 155, 196, 1116, 1591, 1996, 2692
Bse3DI GCAATG 3 cut(s) 408, 1485, 2840
Bse8I GATNNNNATC 2 cut(s) 936, 2177
BseBI CCWGG 3 cut(s) 1889, 2158, 2882
BseDI CCNNGG 3 cut(s) 1308, 1723, 1888
BseGI GGATG 7 cut(s) 584, 1026, 1031, 2495, 2650, 2791, 2866
BseJI GATNNNNATC 2 cut(s) 936, 2177
BseLI CCNNNNNNNGG 8 cut(s) 226, 472, 893, 1130, 1256, 1537, 2555, 2887
BseMI GCAATG 3 cut(s) 408, 1485, 2840
BseMII CTCAG 9 cut(s) 71, 391, 408, 587, 1697, 1818, 2520, 2541, 2594
BseNI ACTGG 6 cut(s) 155, 196, 1116, 1591, 1996, 2692
BseRI GAGGAG 2 cut(s) 649, 951
BseSI GKGCMC 2 cut(s) 2746, 2825
BseXI GCAGC 8 cut(s) 25, 28, 425, 656, 1469, 1633, 1754, 1987
BseYI CCCAGC 3 cut(s) 857, 1197, 2746
Bsh1236I CGCG 1 cut(s) 2040
BshFI GGCC 4 cut(s) 1134, 1530, 1737, 2638
BshNI GGYRCC 3 cut(s) 1390, 1691, 1884
BsiHKAI GWGCWC 2 cut(s) 711, 2825
BsiHKCI CYCGRG 3 cut(s) 1234, 1957, 2708
BsiSI CCGG 5 cut(s) 213, 466, 1308, 1538, 2201
BslFI GGGAC 3 cut(s) 1252, 1483, 1520
BslI CCNNNNNNNGG 8 cut(s) 226, 472, 893, 1130, 1256, 1537, 2555, 2887
BsmAI GTCTC 3 cut(s) 78, 210, 1045
BsmFI GGGAC 3 cut(s) 1252, 1483, 1520
BsmI GAATGC 3 cut(s) 738, 1119, 2141
BsnI GGCC 4 cut(s) 1134, 1530, 1737, 2638
Bso31I GGTCTC 1 cut(s) 210
BsoBI CYCGRG 3 cut(s) 1234, 1957, 2708
Bsp119I TTCGAA 1 cut(s) 1185
Bsp1286I GDGCHC 3 cut(s) 711, 2746, 2825
Bsp1407I TGTACA 2 cut(s) 916, 2371
Bsp143I GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
Bsp1720I GCTNAGC 4 cut(s) 417, 1403, 1706, 2506
BspACI CCGC 5 cut(s) 44, 491, 555, 884, 2635
BspANI GGCC 4 cut(s) 1134, 1530, 1737, 2638
BspCNI CTCAG 9 cut(s) 72, 390, 409, 588, 1698, 1817, 2519, 2542, 2593
BspFNI CGCG 1 cut(s) 2040
BspHI TCATGA 1 cut(s) 2059
BspLI GGNNCC 7 cut(s) 639, 1024, 1392, 1693, 1886, 2243, 2530
BspMAI CTGCAG 4 cut(s) 525, 646, 1623, 2318
BspOI GCTAGC 1 cut(s) 1427
BspPI GGATC 3 cut(s) 2523, 2536, 2690
BspQI GCTCTTC 1 cut(s) 1938
BspT104I TTCGAA 1 cut(s) 1185
BspT107I GGYRCC 3 cut(s) 1390, 1691, 1884
BspTI CTTAAG 2 cut(s) 253, 2384
BspTNI GGTCTC 1 cut(s) 210
BsrDI GCAATG 3 cut(s) 408, 1485, 2840
BsrFI RCCGGY 1 cut(s) 465
BsrGI TGTACA 2 cut(s) 916, 2371
BsrI ACTGG 6 cut(s) 155, 196, 1116, 1591, 1996, 2692
BssAI RCCGGY 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 1308, 1723, 1888
BssMI GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
BssNI GRCGYC 1 cut(s) 1692
Bst2UI CCWGG 3 cut(s) 1889, 2158, 2882
Bst4CI ACNGT 2 cut(s) 788, 2378
Bst6I CTCTTC 3 cut(s) 514, 1938, 2144
BstACI GRCGYC 1 cut(s) 1692
BstAFI CTTAAG 2 cut(s) 253, 2384
BstAPI GCANNNNNTGC 1 cut(s) 201
BstAUI TGTACA 2 cut(s) 916, 2371
BstBI TTCGAA 1 cut(s) 1185
BstC8I GCNNGC 3 cut(s) 1425, 1735, 1760
BstEII GGTNACC 1 cut(s) 1850
BstF5I GGATG 7 cut(s) 584, 1026, 1031, 2495, 2650, 2791, 2866
BstFNI CGCG 1 cut(s) 2040
BstH2I RGCGCY 1 cut(s) 1695
BstHHI GCGC 3 cut(s) 1694, 2040, 2847
BstKTI GATC 7 cut(s) 24, 238, 901, 1435, 2383, 2531, 2685
BstMAI GTCTC 3 cut(s) 78, 210, 1045
BstMBI GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
BstMWI GCNNNNNNNGC 5 cut(s) 201, 742, 1207, 1743, 1764
BstNI CCWGG 3 cut(s) 1889, 2158, 2882
BstNSI RCATGY 2 cut(s) 697, 1762
BstPI GGTNACC 1 cut(s) 1850
BstSCI CCNGG 5 cut(s) 1307, 1536, 1887, 2156, 2880
BstSFI CTRYAG 6 cut(s) 521, 642, 1565, 1619, 2314, 2715
BstSLI GKGCMC 2 cut(s) 2746, 2825
BstUI CGCG 1 cut(s) 2040
BstV1I GCAGC 8 cut(s) 25, 28, 425, 656, 1469, 1633, 1754, 1987
BstV2I GAAGAC 4 cut(s) 378, 1431, 2105, 2472
BstX2I RGATCY 2 cut(s) 1432, 2528
BstXI CCANNNNNNTGG 2 cut(s) 1019, 1598
BstYI RGATCY 2 cut(s) 1432, 2528
BsuI GTATCC 1 cut(s) 2063
BsuRI GGCC 4 cut(s) 1134, 1530, 1737, 2638
BtsCI GGATG 7 cut(s) 584, 1026, 1031, 2495, 2650, 2791, 2866
BtsI GCAGTG 1 cut(s) 2323
BtsIMutI CAGTG 5 cut(s) 77, 1989, 2008, 2323, 2374
Cac8I GCNNGC 3 cut(s) 1425, 1735, 1760
CciI TCATGA 1 cut(s) 2059
CfoI GCGC 3 cut(s) 1694, 2040, 2847
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 615, 1529
CseI GACGC 1 cut(s) 2029
Csp6I GTAC 8 cut(s) 526, 748, 917, 1391, 1954, 2372, 2695, 2893
CviQI GTAC 8 cut(s) 526, 748, 917, 1391, 1954, 2372, 2695, 2893
DinI GGCGCC 1 cut(s) 1693
DpnI GATC 7 cut(s) 23, 237, 900, 1434, 2382, 2530, 2684
DpnII GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
DraI TTTAAA 1 cut(s) 2128
EaeI YGGCCR 1 cut(s) 2636
Eam1104I CTCTTC 3 cut(s) 514, 1938, 2144
EarI CTCTTC 3 cut(s) 514, 1938, 2144
Ecl136II GAGCTC 1 cut(s) 709
Eco147I AGGCCT 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 711
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 615
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 3 cut(s) 2168, 2349, 2485
Eco88I CYCGRG 3 cut(s) 1234, 1957, 2708
Eco91I GGTNACC 1 cut(s) 1850
EcoICRI GAGCTC 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 1529
EcoO65I GGTNACC 1 cut(s) 1850
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 1887, 2156, 2880
EcoT38I GRGCYC 1 cut(s) 711
EgeI GGCGCC 1 cut(s) 1693
EheI GGCGCC 1 cut(s) 1693
FalI AAGNNNNNCTT 2 cut(s) 1340, 1372
FaqI GGGAC 3 cut(s) 1252, 1483, 1520
FauNDI CATATG 1 cut(s) 1969
FbaI TGATCA 1 cut(s) 21
FblI GTMKAC 2 cut(s) 2043, 2197
FokI GGATG 7 cut(s) 591, 1013, 1018, 2482, 2657, 2798, 2873
FriOI GRGCYC 1 cut(s) 711
GlaI GCGC 3 cut(s) 1693, 2039, 2846
GsaI CCCAGC 3 cut(s) 861, 1201, 2750
GsuI CTGGAG 3 cut(s) 491, 1620, 2816
HaeII RGCGCY 1 cut(s) 1695
HaeIII GGCC 4 cut(s) 1134, 1530, 1737, 2638
HapII CCGG 5 cut(s) 213, 466, 1308, 1538, 2201
HgaI GACGC 1 cut(s) 2029
HhaI GCGC 3 cut(s) 1694, 2040, 2847
Hin1I GRCGYC 1 cut(s) 1692
Hin6I GCGC 3 cut(s) 1692, 2038, 2845
HinP1I GCGC 3 cut(s) 1692, 2038, 2845
HincII GTYRAC 1 cut(s) 982
HindII GTYRAC 1 cut(s) 982
HindIII AAGCTT 2 cut(s) 2122, 2668
HpaII CCGG 5 cut(s) 213, 466, 1308, 1538, 2201
HphI GGTGA 9 cut(s) 125, 261, 908, 979, 1106, 1324, 2317, 2474, 2896
Hpy166II GTNNAC 6 cut(s) 982, 2044, 2198, 2374, 2823, 2935
Hpy188I TCNGA 8 cut(s) 127, 200, 718, 1108, 1727, 1880, 2295, 2957
Hpy8I GTNNAC 6 cut(s) 982, 2044, 2198, 2374, 2823, 2935
Hpy99I CGWCG 1 cut(s) 2269
HpyCH4III ACNGT 2 cut(s) 788, 2378
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 742, 1207, 1743, 1764
Hsp92I GRCGYC 1 cut(s) 1692
HspAI GCGC 3 cut(s) 1692, 2038, 2845
KasI GGCGCC 1 cut(s) 1691
KpnI GGTACC 1 cut(s) 1394
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
LguI GCTCTTC 1 cut(s) 1938
LmnI GCTCC 8 cut(s) 229, 706, 714, 1341, 1399, 1479, 2241, 2551
Lsp1109I GCAGC 8 cut(s) 25, 28, 425, 656, 1469, 1633, 1754, 1987
LweI GCATC 6 cut(s) 101, 191, 204, 1275, 2263, 2319
MaeIII GTNAC 6 cut(s) 332, 788, 1007, 1850, 2428, 2623
MalI GATC 7 cut(s) 23, 237, 900, 1434, 2382, 2530, 2684
MboI GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
MflI RGATCY 2 cut(s) 1432, 2528
MhlI GDGCHC 3 cut(s) 711, 2746, 2825
Mly113I GGCGCC 1 cut(s) 1692
MlyI GAGTC 4 cut(s) 1505, 1709, 2483, 2572
MmeI TCCRAC 2 cut(s) 610, 714
MroXI GAANNNNTTC 1 cut(s) 66
MslI CAYNNNNRTG 3 cut(s) 579, 930, 1017
MspA1I CMGCKG 3 cut(s) 416, 1057, 2302
MspCI CTTAAG 2 cut(s) 253, 2384
MspI CCGG 5 cut(s) 213, 466, 1308, 1538, 2201
MspR9I CCNGG 5 cut(s) 1309, 1538, 1889, 2158, 2882
Mva1269I GAATGC 3 cut(s) 738, 1119, 2141
MvaI CCWGG 3 cut(s) 1889, 2158, 2882
MvnI CGCG 1 cut(s) 2040
MwoI GCNNNNNNNGC 5 cut(s) 201, 742, 1207, 1743, 1764
NarI GGCGCC 1 cut(s) 1692
NciI CCSGG 2 cut(s) 1309, 1538
NdeI CATATG 1 cut(s) 1969
NdeII GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
NheI GCTAGC 1 cut(s) 1423
NlaIV GGNNCC 7 cut(s) 639, 1024, 1392, 1693, 1886, 2243, 2530
NmeAIII GCCGAG 2 cut(s) 343, 2415
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 2 cut(s) 697, 1762
NspV TTCGAA 1 cut(s) 1185
PaeI GCATGC 1 cut(s) 1762
PaeR7I CTCGAG 1 cut(s) 2708
PagI TCATGA 1 cut(s) 2059
PceI AGGCCT 1 cut(s) 1134
PciSI GCTCTTC 1 cut(s) 1938
PcsI WCGNNNNNNNCGW 1 cut(s) 2655
PctI GAATGC 3 cut(s) 738, 1119, 2141
PdmI GAANNNNTTC 1 cut(s) 66
PflMI CCANNNNNTGG 1 cut(s) 226
PleI GAGTC 4 cut(s) 1504, 1709, 2483, 2572
PluTI GGCGCC 1 cut(s) 1695
PpsI GAGTC 4 cut(s) 1504, 1709, 2483, 2572
PshBI ATTAAT 1 cut(s) 1820
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 1887, 2156, 2880
PspEI GGTNACC 1 cut(s) 1850
PspFI CCCAGC 3 cut(s) 857, 1197, 2746
PspGI CCWGG 3 cut(s) 1887, 2156, 2880
PspN4I GGNNCC 7 cut(s) 639, 1024, 1392, 1693, 1886, 2243, 2530
PspPI GGNCC 2 cut(s) 615, 1529
PspXI VCTCGAGB 1 cut(s) 2708
PstI CTGCAG 4 cut(s) 525, 646, 1623, 2318
PsuI RGATCY 2 cut(s) 1432, 2528
PvuII CAGCTG 3 cut(s) 416, 1057, 2302
RsaI GTAC 8 cut(s) 527, 749, 918, 1392, 1955, 2373, 2696, 2894
RsaNI GTAC 8 cut(s) 526, 748, 917, 1391, 1954, 2372, 2695, 2893
RseI CAYNNNNRTG 3 cut(s) 579, 930, 1017
SacI GAGCTC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 1938
Sau3AI GATC 7 cut(s) 21, 235, 898, 1432, 2380, 2528, 2682
Sau96I GGNCC 2 cut(s) 615, 1529
SchI GAGTC 4 cut(s) 1505, 1709, 2483, 2572
ScrFI CCNGG 5 cut(s) 1309, 1538, 1889, 2158, 2882
SduI GDGCHC 3 cut(s) 711, 2746, 2825
SfaNI GCATC 6 cut(s) 101, 191, 204, 1275, 2263, 2319
SfcI CTRYAG 6 cut(s) 521, 642, 1565, 1619, 2314, 2715
SfoI GGCGCC 1 cut(s) 1693
Sfr274I CTCGAG 1 cut(s) 2708
SfuI TTCGAA 1 cut(s) 1185
SinI GGWCC 1 cut(s) 615
SlaI CTCGAG 1 cut(s) 2708
SmiMI CAYNNNNRTG 3 cut(s) 579, 930, 1017
SmlI CTYRAG 6 cut(s) 253, 1493, 1637, 2246, 2384, 2708
SmoI CTYRAG 6 cut(s) 253, 1493, 1637, 2246, 2384, 2708
SpeI ACTAGT 1 cut(s) 2452
SphI GCATGC 1 cut(s) 1762
SseBI AGGCCT 1 cut(s) 1134
SsiI CCGC 5 cut(s) 44, 491, 555, 884, 2635
SspDI GGCGCC 1 cut(s) 1691
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 1134
StyD4I CCNGG 5 cut(s) 1307, 1536, 1887, 2156, 2880
TaaI ACNGT 2 cut(s) 788, 2378
TatI WGTACW 4 cut(s) 525, 916, 2371, 2892
TauI GCSGC 3 cut(s) 493, 558, 2638
TscAI CASTG 5 cut(s) 84, 1996, 2008, 2323, 2381
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 8 cut(s) 38, 41, 413, 644, 1482, 1621, 1767, 1975
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 2657
TspRI CASTG 5 cut(s) 84, 1996, 2008, 2323, 2381
Van91I CCANNNNNTGG 1 cut(s) 226
Vha464I CTTAAG 2 cut(s) 253, 2384
VneI GTGCAC 1 cut(s) 2821
VpaK11BI GGWCC 1 cut(s) 615
VspI ATTAAT 1 cut(s) 1820
XapI RAATTY 8 cut(s) 154, 480, 561, 1098, 1516, 1893, 2112, 2167
XbaI TCTAGA 3 cut(s) 142, 1138, 1282
XceI RCATGY 2 cut(s) 697, 1762
XhoI CTCGAG 1 cut(s) 2708
XmiI GTMKAC 2 cut(s) 2043, 2197
XmnI GAANNNNTTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.