Rh2CG008500
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012205
Physical Location & Seq
Forward (+)
744865 .. 748256
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG008500.1

Sequence Viewer

Length: 2985 bp
ATGGGTTTTCTTCGTCATAGAACTTTCATGTTCAATTTTCTCTGTTTGATCATCATTTTCTTGGCTGCTGCGGTGTTTTCAAGCCAAGAAAATGTTCATCAAGCAATGAGAGACAAGGCATCGTTGGTTTCATTCATGTCCGGGATTGTTTCAGACCCTCACCATGCTCTAGAGGACTGGAATTCTAATTTGGACGATGTTCATTTTTGTAATTGGACTGGCATCAGATGCAACAATGCCAGAGACCAAGTTGTGGAGCTTGATCTTAGTAGAAAATCCTTAAGAGGCACTATTTCACCAGCTCTCTCTAATCTTTCTTCCTTAACCATTCTTGATTTGTCAAGCAACTTCTTTGAAGGTCACATTCCAAGAGAGTTAGGCTCTCTTTCTCGGCTCACACAACTCAGCTTGTCTTCAAATCTTCTTGAAGGAAACATTGCAGCTGAGCTTGGTTTCCTTCACAGATTGGTGTATCTTGATTTGGGAAGCAACCGGCTAGGGGGAGAAATTCCAATGCCCCTTTTCTGCAATCACTCTTCTAATTCTCTGCAGTACATAGACTTGTCTAACAATTCTTTAAGCGGCAAAATTCCTTTAATACATGGATGTGAGCTGAAAAAACTGAGGTTTCTTCTACTTTGGTCGAATCATCTAGTTGGACAGGTTCCTGCAGCACTTTCAGACTCCTCGAAACTAGAATGGCTCGACTTAGAATCCAACATGCTAAGCGGGGAGTTGCCATCGGAGATTGTAGCCAGAATGCCAAAGTTGCAGTACCTCTACTTGTCCTATAATGACTTTGTGAGCCATGACGGTAACACTAACCTTGAACCTTTTTTAACCTCTTTACTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGGAATAATCTTGGGGGAGAGATACCGCCTATTATTGGTGATCTTTCAACCAATCTTGTACAAGTTCATTTAGATAGTAATCTTCTTTATGGTTCAATTCCTCCTCACATTTCAAACCTTGTCAACCTCACCCTGTTGAACTTGTCTAGTAACCATTTGAATGGAACCATCCCATCCAAACTATGCCTAATGACAAAGCTGGAACGGGTATATCTCTCGAACAATTCACTCTCTGGTGAAATTCCATCTGAACTGGGTGGCATTTCCCATCTAGGCCTTCTGGATTTATCAAGAAACAAGTTTTCTGGTTCAATCCCAGATAGTTTTGAAAACCTTTCCCAGTTACGAAGGCTATTGCTCTACGAGAATCAACTCTCGGGGACAATACCAGCTAGTCTAGGAAAGTGCATCAACTTAGAGATTCTAGACCTTTCTCACAATCAAATGACCGGGGTGATTCCTAGTGAAGTTGCAGGGTTAAGGAGCTTGAAGTTATACTTGAACTTGTCAAGTAATTACTTAAATGGAGAGGTACCAATGGAGCTAAGCAAAATGGACATGGTGCTAGCAATAGATCTGTCTTCCAATAATCTTTCTGGCACAATCCCATCACAACTTGGAAGCTGCATTGCCCTCGAGTCCCTCAACATTTCATCAAATTCCTTACAAGGCCCTGTCCCGGTTTCAATAGGAAAGTTGCCTTTCCTACAGAAACTTGATGTATCTTCAAACCAGTTGGCTGGACAAATACCAGAATCTCTGCAGCAATCCTTAACTCTCAAGCAACTTAACTTCTCTTTCAACAACTTCTCTGGGAATGTATCAAACAAAGGCGCCTTTTCTTTGCTGAGCTTAGACTCTTTCCTCGGAAATGCTGGCCTCTGTGGCTCAATAAAAGGCATGCCAAGCTGCAAAAAGAAGAAATCTCATCATTTGGCTATACTCTCAGTCCTGCTGTCATTTATTATCACTCCCATTTTTTGCATAGTTGGTTACCCCCTCATGCACAGGTCAAAAATCCGAAGGCACCTGGGAATTTTCGAGGATGGGAAATTGCGAGATGATGATGAAGAAGTAGGAAAAGAAGAGCATAAGTACCCGAGAATCTCATATGAGCAGCTCATTGAAGCCACTGGTGGTTTCAGTGCTTCAAGCCTGATTGGTTCAGGCCGATTTGGGCGCGTCTACAAGGGTATCCTTCATGATAACACAGAAATAGCAGTCAAAGTTTTGGATTTGAAGACAGAAGAAGAGATTTCAGGAAGCTTTAAAAGGGAATGCCAAGTCCTGAAGAGAACCAGACACAGAAATTTGATTAGAATCATCACAACTTGCAGTAAACCGGACTTCAAGGCTCTTGTTCTGCCATTGATGTCTAATGGGAGCCTTGAGAGGCATCTTTACTCGTCGTCATCAAGCCATTGTGGATTGAATCTGATTCAGCTGGTGAGCATCTGCAGTGATGTAGCTGAAGGAGTTGCCTATTTGCACCATCATTCTCCTGTTAGAATTGTACACTGTGATCTTAAACCGAGCAACATTCTACTAGATGATGACATGACAGCTTTGGTTACTGATTTTGGAATTGCAAGACTAGTTAAAGGTGAAGACGAGAGAATTCCCATGAATGACTCGACATCCGTCAGCTCAGCAGATGGCTTGTTATGTGGATCCATTGGCTATATAGCTCCTGAGTATGGAATGGGAAAATGTGCTTCGACTCAGGGAGATGTATTCAGCTTTGGGGTGCTTCTGTTAGAAATTGTTACAGGAAGGCGGCCAACGGATGTCGATTCTCACGAAGGTTCAAGCTTACAGGAATGGATCAAAACCCAGTACCCCCAGAGGCTTGAGCCTATAGTTCAACAAGCAGTAGAAAAGTGTGCCCCAGCTTCAATGCCAAAGCAATATAACAAAATATGGGGGGATGTAGTTCTGGAGTTGATTGAGATTGGTCTTATGTGCACACAGCACAATCCTTCATTGCGCCCAAATATGCAGGATGTTGCTCATGAAATGAGCCAGGTGAAGGAGTACATCTCCAATCCGTCGTCAGCGTTGTTGATTGAAGAAGTGGACACTAGGGTTGATTCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

994

Amino Acids

109.31

Weight (kDa)

5.82

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 38 - 78 1.4e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 207 - 267 1.3e-07 Leucine rich repeat
LRR_8 PF13855 361 - 420 3.3e-06 Leucine rich repeat
LRR_14 PF23598 428 - 578 6.6e-06 Leucine-rich repeat region
LRR_8 PF13855 486 - 541 5.2e-07 Leucine rich repeat
Pkinase PF00069 678 - 966 3.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 680 - 967 1.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1417
AccB1I GGYRCC 3 cut(s) 1417, 1718, 1911
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 1 cut(s) 2070
AccII CGCG 1 cut(s) 2067
AciI CCGC 5 cut(s) 71, 582, 729, 911, 2662
AclWI GGATC 3 cut(s) 2550, 2563, 2717
AcoI YGGCCR 1 cut(s) 2663
AcsI RAATTY 8 cut(s) 181, 507, 588, 1125, 1543, 1920, 2194, 2502
AcuI CTGAAG 2 cut(s) 2195, 2376
AcyI GRCGYC 1 cut(s) 1719
AfaI GTAC 8 cut(s) 554, 776, 945, 1419, 1982, 2400, 2723, 2921
AfiI CCNNNNNNNGG 6 cut(s) 253, 499, 920, 1564, 2582, 2914
AflII CTTAAG 1 cut(s) 280
AhlI ACTAGT 1 cut(s) 2479
AjnI CCWGG 2 cut(s) 1914, 2907
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 173, 205
Alw21I GWGCWC 1 cut(s) 2852
Alw26I GTCTC 2 cut(s) 105, 237
Alw44I GTGCAC 1 cut(s) 2848
AlwI GGATC 3 cut(s) 2550, 2563, 2717
Ama87I CYCGRG 3 cut(s) 1261, 1520, 1984
AoxI GGCC 5 cut(s) 1159, 1555, 1762, 2053, 2663
ApaLI GTGCAC 1 cut(s) 2848
ApeKI GCWGC 8 cut(s) 65, 68, 440, 671, 1509, 1648, 1794, 2002
ApoI RAATTY 8 cut(s) 181, 507, 588, 1125, 1543, 1920, 2194, 2502
Asp700I GAANNNNTTC 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 1417
AspLEI GCGC 3 cut(s) 1721, 2067, 2874
AspS9I GGNCC 1 cut(s) 1556
AsuC2I CCSGG 3 cut(s) 142, 1336, 1565
AsuHPI GGTGA 9 cut(s) 152, 288, 935, 1006, 1133, 1351, 2344, 2501, 2923
AsuNHI GCTAGC 1 cut(s) 1450
AvaI CYCGRG 3 cut(s) 1261, 1520, 1984
BaeGI GKGCMC 2 cut(s) 2773, 2852
BaeI ACNNNNGTAYC 2 cut(s) 2062, 2095
BamHI GGATCC 1 cut(s) 2555
BanI GGYRCC 3 cut(s) 1417, 1718, 1911
BbsI GAAGAC 4 cut(s) 405, 1458, 2132, 2499
Bbv12I GWGCWC 1 cut(s) 2852
BbvI GCAGC 8 cut(s) 52, 55, 452, 683, 1496, 1660, 1781, 2014
BccI CCATC 9 cut(s) 748, 1061, 1066, 1138, 1161, 1501, 1925, 2385, 2534
BcgI CGANNNNNNTGC 2 cut(s) 1501, 1535
BciT130I CCWGG 2 cut(s) 1916, 2909
BciVI GTATCC 1 cut(s) 2090
BclI TGATCA 1 cut(s) 48
BcnI CCSGG 3 cut(s) 142, 1336, 1565
BcoDI GTCTC 2 cut(s) 105, 237
BcuI ACTAGT 1 cut(s) 2479
BfmI CTRYAG 6 cut(s) 548, 669, 1592, 1646, 2341, 2742
BfoI RGCGCY 1 cut(s) 1722
BfrI CTTAAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 2090
BglI GCCNNNNNGGC 1 cut(s) 1770
BglII AGATCT 1 cut(s) 1459
BlpI GCTNAGC 5 cut(s) 444, 725, 1430, 1733, 2533
Bme1390I CCNGG 5 cut(s) 142, 1336, 1565, 1916, 2909
BmeT110I CYCGRG 3 cut(s) 1261, 1520, 1984
BmgT120I GGNCC 1 cut(s) 1556
BmiI GGNNCC 7 cut(s) 666, 1051, 1419, 1720, 1913, 2270, 2557
BmrFI CCNGG 5 cut(s) 142, 1336, 1565, 1916, 2909
BmrI ACTGGG 3 cut(s) 1148, 1219, 2713
BmsI GCATC 6 cut(s) 128, 218, 231, 1302, 2290, 2346
BmtI GCTAGC 1 cut(s) 1454
BmuI ACTGGG 3 cut(s) 1148, 1219, 2713
BoxI GACNNNNGTC 1 cut(s) 2525
BpiI GAAGAC 4 cut(s) 405, 1458, 2132, 2499
BplI GAGNNNNNCTC 4 cut(s) 365, 397, 2909, 2941
BpmI CTGGAG 1 cut(s) 2843
Bpu1102I GCTNAGC 5 cut(s) 444, 725, 1430, 1733, 2533
BpuEI CTTGAG 3 cut(s) 1649, 2294, 2756
BpuMI CCSGG 3 cut(s) 142, 1336, 1565
BsaBI GATNNNNATC 2 cut(s) 963, 2204
BsaHI GRCGYC 1 cut(s) 1719
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 1335, 1750, 1915
BsaWI WCCGGW 1 cut(s) 2227
Bsc4I CCNNNNNNNGG 6 cut(s) 253, 499, 920, 1564, 2582, 2914
Bse118I RCCGGY 1 cut(s) 492
Bse1I ACTGG 7 cut(s) 182, 223, 1143, 1225, 1618, 2023, 2719
Bse3DI GCAATG 4 cut(s) 111, 435, 1512, 2867
Bse8I GATNNNNATC 2 cut(s) 963, 2204
BseBI CCWGG 2 cut(s) 1916, 2909
BseDI CCNNGG 3 cut(s) 1335, 1750, 1915
BseGI GGATG 8 cut(s) 611, 1053, 1058, 1936, 2522, 2677, 2818, 2893
BseJI GATNNNNATC 2 cut(s) 963, 2204
BseLI CCNNNNNNNGG 6 cut(s) 253, 499, 920, 1564, 2582, 2914
BseMI GCAATG 4 cut(s) 111, 435, 1512, 2867
BseMII CTCAG 8 cut(s) 418, 435, 614, 1724, 1845, 2547, 2568, 2621
BseNI ACTGG 7 cut(s) 182, 223, 1143, 1225, 1618, 2023, 2719
BseRI GAGGAG 2 cut(s) 676, 978
BseSI GKGCMC 2 cut(s) 2773, 2852
BseXI GCAGC 8 cut(s) 52, 55, 452, 683, 1496, 1660, 1781, 2014
BseYI CCCAGC 2 cut(s) 884, 2773
Bsh1236I CGCG 1 cut(s) 2067
BshFI GGCC 5 cut(s) 1161, 1557, 1764, 2055, 2665
BshNI GGYRCC 3 cut(s) 1417, 1718, 1911
BsiHKAI GWGCWC 1 cut(s) 2852
BsiHKCI CYCGRG 3 cut(s) 1261, 1520, 1984
BsiSI CCGG 5 cut(s) 141, 493, 1335, 1565, 2228
BslFI GGGAC 3 cut(s) 1279, 1510, 1547
BslI CCNNNNNNNGG 6 cut(s) 253, 499, 920, 1564, 2582, 2914
BsmAI GTCTC 2 cut(s) 105, 237
BsmFI GGGAC 3 cut(s) 1279, 1510, 1547
BsmI GAATGC 2 cut(s) 765, 2168
BsnI GGCC 5 cut(s) 1161, 1557, 1764, 2055, 2665
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 3 cut(s) 1261, 1520, 1984
Bsp1286I GDGCHC 2 cut(s) 2773, 2852
Bsp1407I TGTACA 2 cut(s) 943, 2398
Bsp143I GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
Bsp1720I GCTNAGC 5 cut(s) 444, 725, 1430, 1733, 2533
BspACI CCGC 5 cut(s) 71, 582, 729, 911, 2662
BspANI GGCC 5 cut(s) 1161, 1557, 1764, 2055, 2665
BspCNI CTCAG 8 cut(s) 417, 436, 615, 1725, 1844, 2546, 2569, 2620
BspFNI CGCG 1 cut(s) 2067
BspHI TCATGA 2 cut(s) 2086, 2896
BspLI GGNNCC 7 cut(s) 666, 1051, 1419, 1720, 1913, 2270, 2557
BspMAI CTGCAG 4 cut(s) 552, 673, 1650, 2345
BspOI GCTAGC 1 cut(s) 1454
BspPI GGATC 3 cut(s) 2550, 2563, 2717
BspQI GCTCTTC 1 cut(s) 1965
BspT107I GGYRCC 3 cut(s) 1417, 1718, 1911
BspTI CTTAAG 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 4 cut(s) 111, 435, 1512, 2867
BsrFI RCCGGY 1 cut(s) 492
BsrGI TGTACA 2 cut(s) 943, 2398
BsrI ACTGG 7 cut(s) 182, 223, 1143, 1225, 1618, 2023, 2719
BssAI RCCGGY 1 cut(s) 492
BssECI CCNNGG 3 cut(s) 1335, 1750, 1915
BssMI GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
BssNI GRCGYC 1 cut(s) 1719
Bst2UI CCWGG 2 cut(s) 1916, 2909
Bst4CI ACNGT 2 cut(s) 815, 2405
Bst6I CTCTTC 4 cut(s) 541, 1965, 2130, 2171
BstACI GRCGYC 1 cut(s) 1719
BstAFI CTTAAG 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 228
BstAUI TGTACA 2 cut(s) 943, 2398
BstC8I GCNNGC 3 cut(s) 1452, 1762, 1787
BstEII GGTNACC 1 cut(s) 1877
BstF5I GGATG 8 cut(s) 611, 1053, 1058, 1936, 2522, 2677, 2818, 2893
BstFNI CGCG 1 cut(s) 2067
BstH2I RGCGCY 1 cut(s) 1722
BstHHI GCGC 3 cut(s) 1721, 2067, 2874
BstKTI GATC 7 cut(s) 51, 265, 928, 1462, 2410, 2558, 2712
BstMAI GTCTC 2 cut(s) 105, 237
BstMBI GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
BstMWI GCNNNNNNNGC 4 cut(s) 228, 769, 1770, 1791
BstNI CCWGG 2 cut(s) 1916, 2909
BstNSI RCATGY 2 cut(s) 724, 1789
BstPAI GACNNNNGTC 1 cut(s) 2525
BstPI GGTNACC 1 cut(s) 1877
BstSCI CCNGG 5 cut(s) 140, 1334, 1563, 1914, 2907
BstSFI CTRYAG 6 cut(s) 548, 669, 1592, 1646, 2341, 2742
BstSLI GKGCMC 2 cut(s) 2773, 2852
BstUI CGCG 1 cut(s) 2067
BstV1I GCAGC 8 cut(s) 52, 55, 452, 683, 1496, 1660, 1781, 2014
BstV2I GAAGAC 4 cut(s) 405, 1458, 2132, 2499
BstX2I RGATCY 2 cut(s) 1459, 2555
BstXI CCANNNNNNTGG 2 cut(s) 1046, 1625
BstYI RGATCY 2 cut(s) 1459, 2555
BsuI GTATCC 1 cut(s) 2090
BsuRI GGCC 5 cut(s) 1161, 1557, 1764, 2055, 2665
BtsCI GGATG 8 cut(s) 611, 1053, 1058, 1936, 2522, 2677, 2818, 2893
BtsI GCAGTG 1 cut(s) 2350
BtsIMutI CAGTG 4 cut(s) 2016, 2035, 2350, 2401
Cac8I GCNNGC 3 cut(s) 1452, 1762, 1787
CciI TCATGA 2 cut(s) 2086, 2896
CfoI GCGC 3 cut(s) 1721, 2067, 2874
Cfr10I RCCGGY 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 1556
CseI GACGC 1 cut(s) 2056
Csp6I GTAC 8 cut(s) 553, 775, 944, 1418, 1981, 2399, 2722, 2920
CviQI GTAC 8 cut(s) 553, 775, 944, 1418, 1981, 2399, 2722, 2920
DinI GGCGCC 1 cut(s) 1720
DpnI GATC 7 cut(s) 50, 264, 927, 1461, 2409, 2557, 2711
DpnII GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
DraI TTTAAA 1 cut(s) 2155
EaeI YGGCCR 1 cut(s) 2663
Eam1104I CTCTTC 4 cut(s) 541, 1965, 2130, 2171
EarI CTCTTC 4 cut(s) 541, 1965, 2130, 2171
Eco147I AGGCCT 1 cut(s) 1161
Eco31I GGTCTC 1 cut(s) 237
Eco57I CTGAAG 2 cut(s) 2195, 2376
Eco88I CYCGRG 3 cut(s) 1261, 1520, 1984
Eco91I GGTNACC 1 cut(s) 1877
EcoO109I RGGNCCY 1 cut(s) 1556
EcoO65I GGTNACC 1 cut(s) 1877
EcoRI GAATTC 2 cut(s) 181, 2502
EcoRII CCWGG 2 cut(s) 1914, 2907
EgeI GGCGCC 1 cut(s) 1720
EheI GGCGCC 1 cut(s) 1720
FalI AAGNNNNNCTT 2 cut(s) 1367, 1399
FaqI GGGAC 3 cut(s) 1279, 1510, 1547
FauI CCCGC 1 cut(s) 722
FauNDI CATATG 1 cut(s) 1996
FbaI TGATCA 1 cut(s) 48
FblI GTMKAC 1 cut(s) 2070
FokI GGATG 8 cut(s) 618, 1040, 1045, 1943, 2509, 2684, 2825, 2900
GlaI GCGC 3 cut(s) 1720, 2066, 2873
GsaI CCCAGC 2 cut(s) 888, 2777
GsuI CTGGAG 1 cut(s) 2843
HaeII RGCGCY 1 cut(s) 1722
HaeIII GGCC 5 cut(s) 1161, 1557, 1764, 2055, 2665
HapII CCGG 5 cut(s) 141, 493, 1335, 1565, 2228
HgaI GACGC 1 cut(s) 2056
HhaI GCGC 3 cut(s) 1721, 2067, 2874
Hin1I GRCGYC 1 cut(s) 1719
Hin6I GCGC 3 cut(s) 1719, 2065, 2872
HinP1I GCGC 3 cut(s) 1719, 2065, 2872
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HindIII AAGCTT 2 cut(s) 2149, 2695
HpaII CCGG 5 cut(s) 141, 493, 1335, 1565, 2228
HphI GGTGA 9 cut(s) 152, 288, 935, 1006, 1133, 1351, 2344, 2501, 2923
Hpy166II GTNNAC 6 cut(s) 1009, 2071, 2225, 2401, 2850, 2962
Hpy188I TCNGA 9 cut(s) 154, 227, 682, 745, 1135, 1754, 1907, 2322, 2984
Hpy8I GTNNAC 6 cut(s) 1009, 2071, 2225, 2401, 2850, 2962
Hpy99I CGWCG 2 cut(s) 2296, 2938
HpyCH4III ACNGT 2 cut(s) 815, 2405
HpyF10VI GCNNNNNNNGC 4 cut(s) 228, 769, 1770, 1791
Hsp92I GRCGYC 1 cut(s) 1719
HspAI GCGC 3 cut(s) 1719, 2065, 2872
KasI GGCGCC 1 cut(s) 1718
KpnI GGTACC 1 cut(s) 1421
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
LguI GCTCTTC 1 cut(s) 1965
LmnI GCTCC 5 cut(s) 256, 1368, 1426, 2268, 2578
Lsp1109I GCAGC 8 cut(s) 52, 55, 452, 683, 1496, 1660, 1781, 2014
LweI GCATC 6 cut(s) 128, 218, 231, 1302, 2290, 2346
MaeIII GTNAC 7 cut(s) 359, 815, 1034, 1227, 1877, 2455, 2650
MalI GATC 7 cut(s) 50, 264, 927, 1461, 2409, 2557, 2711
MboI GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
MflI RGATCY 2 cut(s) 1459, 2555
MhlI GDGCHC 2 cut(s) 2773, 2852
Mly113I GGCGCC 1 cut(s) 1719
MlyI GAGTC 5 cut(s) 677, 1532, 1736, 2510, 2599
MmeI TCCRAC 2 cut(s) 637, 741
MroXI GAANNNNTTC 1 cut(s) 93
MslI CAYNNNNRTG 2 cut(s) 606, 1044
MspA1I CMGCKG 2 cut(s) 443, 2329
MspCI CTTAAG 1 cut(s) 280
MspI CCGG 5 cut(s) 141, 493, 1335, 1565, 2228
MspR9I CCNGG 5 cut(s) 142, 1336, 1565, 1916, 2909
Mva1269I GAATGC 2 cut(s) 765, 2168
MvaI CCWGG 2 cut(s) 1916, 2909
MvnI CGCG 1 cut(s) 2067
MwoI GCNNNNNNNGC 4 cut(s) 228, 769, 1770, 1791
NarI GGCGCC 1 cut(s) 1719
NciI CCSGG 3 cut(s) 142, 1336, 1565
NdeI CATATG 1 cut(s) 1996
NdeII GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
NheI GCTAGC 1 cut(s) 1450
NlaIV GGNNCC 7 cut(s) 666, 1051, 1419, 1720, 1913, 2270, 2557
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 2 cut(s) 724, 1789
PaeI GCATGC 1 cut(s) 1789
PaeR7I CTCGAG 1 cut(s) 1520
PagI TCATGA 2 cut(s) 2086, 2896
PceI AGGCCT 1 cut(s) 1161
PciSI GCTCTTC 1 cut(s) 1965
PcsI WCGNNNNNNNCGW 1 cut(s) 2682
PctI GAATGC 2 cut(s) 765, 2168
PdmI GAANNNNTTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 253
PfoI TCCNGGA 1 cut(s) 140
PleI GAGTC 5 cut(s) 677, 1531, 1736, 2510, 2599
PluTI GGCGCC 1 cut(s) 1722
PpsI GAGTC 5 cut(s) 677, 1531, 1736, 2510, 2599
PshAI GACNNNNGTC 1 cut(s) 2525
Psp6I CCWGG 2 cut(s) 1914, 2907
PspEI GGTNACC 1 cut(s) 1877
PspFI CCCAGC 2 cut(s) 884, 2773
PspGI CCWGG 2 cut(s) 1914, 2907
PspN4I GGNNCC 7 cut(s) 666, 1051, 1419, 1720, 1913, 2270, 2557
PspPI GGNCC 1 cut(s) 1556
PspXI VCTCGAGB 1 cut(s) 1520
PstI CTGCAG 4 cut(s) 552, 673, 1650, 2345
PsuI RGATCY 2 cut(s) 1459, 2555
PvuII CAGCTG 2 cut(s) 443, 2329
RsaI GTAC 8 cut(s) 554, 776, 945, 1419, 1982, 2400, 2723, 2921
RsaNI GTAC 8 cut(s) 553, 775, 944, 1418, 1981, 2399, 2722, 2920
RseI CAYNNNNRTG 2 cut(s) 606, 1044
SapI GCTCTTC 1 cut(s) 1965
Sau3AI GATC 7 cut(s) 48, 262, 925, 1459, 2407, 2555, 2709
Sau96I GGNCC 1 cut(s) 1556
SchI GAGTC 5 cut(s) 677, 1532, 1736, 2510, 2599
ScrFI CCNGG 5 cut(s) 142, 1336, 1565, 1916, 2909
SduI GDGCHC 2 cut(s) 2773, 2852
SfaNI GCATC 6 cut(s) 128, 218, 231, 1302, 2290, 2346
SfcI CTRYAG 6 cut(s) 548, 669, 1592, 1646, 2341, 2742
SfoI GGCGCC 1 cut(s) 1720
Sfr274I CTCGAG 1 cut(s) 1520
SlaI CTCGAG 1 cut(s) 1520
SmiMI CAYNNNNRTG 2 cut(s) 606, 1044
SmlI CTYRAG 5 cut(s) 280, 1520, 1664, 2273, 2735
SmoI CTYRAG 5 cut(s) 280, 1520, 1664, 2273, 2735
SpeI ACTAGT 1 cut(s) 2479
SphI GCATGC 1 cut(s) 1789
SseBI AGGCCT 1 cut(s) 1161
SsiI CCGC 5 cut(s) 71, 582, 729, 911, 2662
SspDI GGCGCC 1 cut(s) 1718
StuI AGGCCT 1 cut(s) 1161
StyD4I CCNGG 5 cut(s) 140, 1334, 1563, 1914, 2907
TaaI ACNGT 2 cut(s) 815, 2405
TaqI TCGA 9 cut(s) 644, 689, 705, 1103, 1521, 1926, 2519, 2603, 2676
TatI WGTACW 4 cut(s) 552, 943, 2398, 2919
TauI GCSGC 2 cut(s) 585, 2665
TscAI CASTG 4 cut(s) 2023, 2035, 2350, 2408
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 8 cut(s) 65, 68, 440, 671, 1509, 1648, 1794, 2002
Tsp45I GTSAC 1 cut(s) 359
TspGWI ACGGA 3 cut(s) 2515, 2684, 2922
TspRI CASTG 4 cut(s) 2023, 2035, 2350, 2408
Van91I CCANNNNNTGG 1 cut(s) 253
Vha464I CTTAAG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 2848
XapI RAATTY 8 cut(s) 181, 507, 588, 1125, 1543, 1920, 2194, 2502
XbaI TCTAGA 2 cut(s) 169, 1309
XceI RCATGY 2 cut(s) 724, 1789
XhoI CTCGAG 1 cut(s) 1520
XmiI GTMKAC 1 cut(s) 2070
XmnI GAANNNNTTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.