MD02G1005800.v1.1
HSP70 Family

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008186
Physical Location & Seq
Reverse (-)
400695 .. 402656
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005800.v1.1.491

Sequence Viewer

Length: 1962 bp
ATGGCGGCCAAGACCGAAGGAAAGGCTATCGGCATCGACCTCGGCACAACTTACAGCTGCGTTGGGGTCTGGCAGAACGACCGAGTCGAGATCATCGCCAATGATCAGGGCAACCGGACAACCCCGTCCTACGTGGCGTTCACCGACACCGAGCGGCTTATCGGTGACGCTGCGAAGAACCAAGTGGCGATGAACCCGCATAACACAGTATTTGACGCGAAACGGCTCATCGGGAGGAAGTTCTCCGACGCGTCGGTGCAGAGCGACATGAAGCTCTGGCCTTTTAAGGTTATTCCGGGCCCCGGAGACAAGCCCTTGATCGTCGTGAACTATAAGGGAGAGGAGAAGAAGTTCTCGGCGGAGGAGATTTCGTCGATGGTGCTGACGAAGATGAGGGAGATCGCGGAGGCGTATCTGGGTCAGACGGTGAAGAACGCTGTCGTGACTGTCCCTGCCTACTTCAACGACTCTCAGCGGCAGGCCACCAAGGACGCTGGTGCGATTTCCGGGCTCAACGTCATGCGGATCATCAACGAGCCGACTGCCGCCGCCATCGCCTACGGGCTTGATAAGAAGGCGTCCAGAAAGGGGGAGCAGAACGTCCTGATTTTCGACCTTGGCGGAGGGACTTTCGATGTCTCGCTTCTGACGATCGAGGAGGGGATTTTCGAGGTGAAGGCCACCGCCGGAGACACCCATCTGGGTGGAGAGGACTTCGACAATAGGATGGTGAATCACTTCGTGTCGGAGTTCCGGCGGAAGCATAAGAAGGATATTAGCGGAAACGCCAGGGCGTTGCGGCGGCTGAGGACGGCGTGCGAGCGGGCGAAGAGGACTCTGTCTTCCACTTCCCAGACAACAATCGAAATTGATTCTCTGTACGAAGGGATCGACTTCTACGCGACGATTACCCGGGCGAGGTTCGAGGAAATGAACATGGATTTGTTCAGGAAGTGTATGGAGCCGGTAGAGAAATGTTTGAGGGACGCGAAGATCGACAAGTCGCTGGTTCATGAAGTTGTTCTCGTCGGCGGGTCGACGAGGATCCCGAAAGTCCAGCAGCTTTTGCAGGATTTTTTTAACGGGAAGGAGCTCTGTAAGAGTATAAACCCCGATGAGGCTGTCGCTTACGGCGCGGCGGTACAAGCGGCGATTTTGAGCGGCGATGGGGATGAGAAAGTGCAGGACTTGCTGCTGCTCGATGTCACGCCACTCAGTCTCGGGCTGGAGACTGCCGGCGGTGTTATGACGGTGCTGATTCCGAGGAACACGACTATTCCGACGAAGAAAGAGCAGATCTTCTCGACTTACTCGGATAATCAGCCGGGAGTTTTGATTCAGGTTTATGAAGGAGAGCGGGCGAGGACGAAGGATAATAATCTGTTGGGGAAATTCGAGCTCACCGGAATCCCTCCTGCTCCCAGGGGCGTGCCTCAGATCAATGTGTGTTTCGACATTGATGCGAATGGGATCTTGAACGTGTCGGCGGAGGACAAGACGGCGGGGGTGAAGAATAAAATCACGATTACGAATGACAAGGGCCGGCTGAGCAAGGAGGAGATAGAGAAGATGGTACAGGATGCGGAGAAGTACAGGGCGGAAGACGAGGAGGTGAAGAAGAAGGTGGAGGCAAAGAATGCGTTGGAGAATTATGCGTACAACATGAGGAACACCGTGAGGGATGATAAATTTGCCGGGAAATTGGACCCGGCGGACAAGGAGAAGATCGAGAAGGCGGTGGACGACGCGATTAATTGGCTGGAGGGGAACCAGTTGGCAGAAGTTGATGAGTTGGAGGACAAGTTGAAGGAGCTGGAAGGGCTGTGCAATCCTATTATTGCAAAGATGTACCAGGGTGCCGGCGGCGCTGGAGGTGGCATGCCGATGGGTGATGACATGCCTGGTGGCGCTTCCACTGGCTCCGCCAGTGGACCCGGCCCCAAGATTGAGGAGGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

654

Amino Acids

71.44

Weight (kDa)

5.22

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 618 1.6e-261 Hsp70 protein
MreB_Mbl PF06723 141 - 380 5e-14 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 124
AccB1I GGYRCC 1 cut(s) 1856
AccBSI CCGCTC 4 cut(s) 154, 823, 1161, 1357
AccI GTMKAC 1 cut(s) 1037
AccII CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
AclWI GGATC 5 cut(s) 533, 896, 1039, 1052, 1478
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 1391, 1688
AcyI GRCGYC 1 cut(s) 578
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 6 cut(s) 881, 1143, 1575, 1592, 1658, 1850
AfiI CCNNNNNNNGG 4 cut(s) 302, 588, 918, 1117
AflIII ACRYGT 2 cut(s) 249, 1479
AgsI TTSAA 3 cut(s) 463, 1477, 1807
AjnI CCWGG 4 cut(s) 788, 1421, 1851, 1900
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 6 cut(s) 57, 274, 1063, 1093, 1399, 1813
AluI AGCT 6 cut(s) 57, 274, 1063, 1093, 1399, 1813
Alw21I GWGCWC 2 cut(s) 1095, 1401
Alw26I GTCTC 5 cut(s) 300, 643, 684, 1223, 1223
AlwI GGATC 5 cut(s) 533, 896, 1039, 1052, 1478
Ama87I CYCGRG 2 cut(s) 912, 1220
AoxI GGCC 7 cut(s) 6, 278, 298, 480, 678, 1540, 1936
ApaI GGGCCC 1 cut(s) 302
ApeKI GCWGC 5 cut(s) 57, 170, 1060, 1192, 1195
ApoI RAATTY 2 cut(s) 1391, 1688
AseI ATTAAT 1 cut(s) 1752
Asp700I GAANNNNTTC 3 cut(s) 350, 737, 1020
AspLEI GCGC 3 cut(s) 1136, 1868, 1910
AspS9I GGNCC 6 cut(s) 298, 299, 1540, 1705, 1931, 1937
AsuC2I CCSGG 9 cut(s) 297, 303, 508, 913, 914, 1326, 1696, 1709, 1935
AsuHPI GGTGA 9 cut(s) 133, 176, 439, 685, 742, 1393, 1519, 1624, 1901
AvaI CYCGRG 2 cut(s) 912, 1220
AvaII GGWCC 2 cut(s) 1705, 1931
BaeGI GKGCMC 1 cut(s) 302
BamHI GGATCC 1 cut(s) 1044
BanI GGYRCC 1 cut(s) 1856
BanII GRGCYC 4 cut(s) 302, 513, 1095, 1401
BbsI GAAGAC 2 cut(s) 834, 1608
Bbv12I GWGCWC 2 cut(s) 1095, 1401
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 5 cut(s) 44, 157, 1072, 1179, 1182
BccI CCATC 7 cut(s) 370, 560, 705, 721, 1160, 1564, 1879
BceAI ACGGC 4 cut(s) 239, 828, 1147, 1515
BcgI CGANNNNNNTGC 6 cut(s) 22, 56, 524, 558, 1442, 1476
BciT130I CCWGG 4 cut(s) 790, 1423, 1853, 1902
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 9 cut(s) 297, 303, 508, 913, 914, 1326, 1696, 1709, 1935
BcoDI GTCTC 5 cut(s) 300, 643, 684, 1223, 1223
BfoI RGCGCY 2 cut(s) 1869, 1911
BglII AGATCT 1 cut(s) 1296
BlpI GCTNAGC 1 cut(s) 1547
Bme18I GGWCC 2 cut(s) 1705, 1931
BmeT110I CYCGRG 2 cut(s) 912, 1220
BmgT120I GGNCC 6 cut(s) 298, 299, 1540, 1705, 1931, 1937
BmsI GCATC 3 cut(s) 42, 1450, 1570
BpiI GAAGAC 2 cut(s) 834, 1608
BpmI CTGGAG 3 cut(s) 1247, 1781, 1890
Bpu10I CCTNAGC 1 cut(s) 806
Bpu1102I GCTNAGC 1 cut(s) 1547
BpuMI CCSGG 9 cut(s) 297, 303, 508, 913, 914, 1326, 1696, 1709, 1935
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 1377
BsaHI GRCGYC 1 cut(s) 578
BsaWI WCCGGW 2 cut(s) 114, 1403
BsaXI ACNNNNNCTCC 6 cut(s) 335, 356, 365, 386, 1787, 1817
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 588, 918, 1117
Bse118I RCCGGY 4 cut(s) 964, 1235, 1542, 1859
Bse1I ACTGG 3 cut(s) 1771, 1921, 1926
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 4 cut(s) 790, 1423, 1853, 1902
BseGI GGATG 4 cut(s) 732, 1177, 1585, 1687
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 4 cut(s) 302, 588, 918, 1117
BseMII CTCAG 5 cut(s) 485, 797, 1228, 1448, 1538
BseNI ACTGG 3 cut(s) 1771, 1921, 1926
BseRI GAGGAG 5 cut(s) 356, 377, 671, 1571, 1622
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 5 cut(s) 44, 157, 1072, 1179, 1182
BsgI GTGCAG 2 cut(s) 278, 1202
Bsh1236I CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
Bsh1285I CGRYCG 2 cut(s) 82, 654
BshFI GGCC 7 cut(s) 8, 280, 300, 482, 680, 1542, 1938
BshNI GGYRCC 1 cut(s) 1856
BsiEI CGRYCG 2 cut(s) 82, 654
BsiHKAI GWGCWC 2 cut(s) 1095, 1401
BsiHKCI CYCGRG 2 cut(s) 912, 1220
BslFI GGGAC 3 cut(s) 434, 640, 998
BslI CCNNNNNNNGG 4 cut(s) 302, 588, 918, 1117
BsmAI GTCTC 5 cut(s) 300, 643, 684, 1223, 1223
BsmFI GGGAC 3 cut(s) 434, 640, 998
BsmI GAATGC 1 cut(s) 1642
BsnI GGCC 7 cut(s) 8, 280, 300, 482, 680, 1542, 1938
BsoBI CYCGRG 2 cut(s) 912, 1220
Bsp120I GGGCCC 1 cut(s) 298
Bsp1286I GDGCHC 4 cut(s) 302, 513, 1095, 1401
Bsp1720I GCTNAGC 1 cut(s) 1547
BspANI GGCC 7 cut(s) 8, 280, 300, 482, 680, 1542, 1938
BspCNI CTCAG 5 cut(s) 484, 798, 1227, 1447, 1539
BspFNI CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
BspHI TCATGA 1 cut(s) 1012
BspPI GGATC 5 cut(s) 533, 896, 1039, 1052, 1478
BspT107I GGYRCC 1 cut(s) 1856
BsrBI CCGCTC 4 cut(s) 154, 823, 1161, 1357
BsrFI RCCGGY 4 cut(s) 964, 1235, 1542, 1859
BsrI ACTGG 3 cut(s) 1771, 1921, 1926
BssAI RCCGGY 4 cut(s) 964, 1235, 1542, 1859
BssNI GRCGYC 1 cut(s) 578
BssT1I CCWWGG 2 cut(s) 486, 616
Bst2UI CCWGG 4 cut(s) 790, 1423, 1853, 1902
Bst4CI ACNGT 5 cut(s) 208, 427, 448, 1252, 1675
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 578
BstAPI GCANNNNNTGC 3 cut(s) 1066, 1189, 1637
BstBAI YACGTR 1 cut(s) 133
BstDEI CTNAG 5 cut(s) 471, 806, 1214, 1434, 1547
BstF5I GGATG 4 cut(s) 732, 1177, 1585, 1687
BstFNI CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
BstH2I RGCGCY 2 cut(s) 1869, 1911
BstHHI GCGC 3 cut(s) 1136, 1868, 1910
BstMAI GTCTC 5 cut(s) 300, 643, 684, 1223, 1223
BstMCI CGRYCG 2 cut(s) 82, 654
BstMWI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1189, 1548, 1637, 1819, 1865
BstNI CCWGG 4 cut(s) 790, 1423, 1853, 1902
BstNSI RCATGY 2 cut(s) 1882, 1900
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
BstV1I GCAGC 5 cut(s) 44, 157, 1072, 1179, 1182
BstV2I GAAGAC 2 cut(s) 834, 1608
BstX2I RGATCY 3 cut(s) 1044, 1296, 1470
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 3 cut(s) 1044, 1296, 1470
BsuRI GGCC 7 cut(s) 8, 280, 300, 482, 680, 1542, 1938
BtgZI GCGATG 4 cut(s) 79, 203, 538, 1179
BtsCI GGATG 4 cut(s) 732, 1177, 1585, 1687
BtsIMutI CAGTG 2 cut(s) 1914, 1933
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 3 cut(s) 1136, 1868, 1910
Cfr10I RCCGGY 4 cut(s) 964, 1235, 1542, 1859
Cfr13I GGNCC 6 cut(s) 298, 299, 1540, 1705, 1931, 1937
Cfr9I CCCGGG 1 cut(s) 912
CseI GACGC 8 cut(s) 176, 224, 240, 257, 500, 567, 995, 1754
Csp6I GTAC 6 cut(s) 880, 1142, 1574, 1591, 1657, 1849
CviAII CATG 7 cut(s) 268, 520, 937, 1013, 1663, 1879, 1897
CviQI GTAC 6 cut(s) 880, 1142, 1574, 1591, 1657, 1849
DdeI CTNAG 5 cut(s) 471, 806, 1214, 1434, 1547
DraIII CACNNNGTG 1 cut(s) 742
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
EciI GGCGGA 7 cut(s) 374, 636, 772, 1502, 1613, 1727, 1912
Ecl136II GAGCTC 2 cut(s) 1093, 1399
Eco130I CCWWGG 2 cut(s) 486, 616
Eco24I GRGCYC 4 cut(s) 302, 513, 1095, 1401
Eco47I GGWCC 2 cut(s) 1705, 1931
Eco53kI GAGCTC 2 cut(s) 1093, 1399
Eco88I CYCGRG 2 cut(s) 912, 1220
EcoICRI GAGCTC 2 cut(s) 1093, 1399
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 4 cut(s) 788, 1421, 1851, 1900
EcoT14I CCWWGG 2 cut(s) 486, 616
EcoT38I GRGCYC 4 cut(s) 302, 513, 1095, 1401
ErhI CCWWGG 2 cut(s) 486, 616
FaeI CATG 7 cut(s) 271, 523, 940, 1016, 1666, 1882, 1900
FaqI GGGAC 3 cut(s) 434, 640, 998
FatI CATG 7 cut(s) 267, 519, 936, 1012, 1662, 1878, 1896
FauI CCCGC 5 cut(s) 204, 816, 1025, 1350, 1495
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 1037
FokI GGATG 4 cut(s) 739, 1184, 1592, 1694
FriOI GRGCYC 4 cut(s) 302, 513, 1095, 1401
GlaI GCGC 3 cut(s) 1135, 1867, 1909
GsuI CTGGAG 3 cut(s) 1247, 1781, 1890
HaeII RGCGCY 2 cut(s) 1869, 1911
HaeIII GGCC 7 cut(s) 8, 280, 300, 482, 680, 1542, 1938
HgaI GACGC 8 cut(s) 176, 224, 240, 257, 500, 567, 995, 1754
HhaI GCGC 3 cut(s) 1136, 1868, 1910
Hin1I GRCGYC 1 cut(s) 578
Hin1II CATG 7 cut(s) 271, 523, 940, 1016, 1666, 1882, 1900
Hin6I GCGC 3 cut(s) 1134, 1866, 1908
HinP1I GCGC 3 cut(s) 1134, 1866, 1908
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 8 cut(s) 84, 467, 733, 835, 872, 1258, 1336, 1407
HphI GGTGA 9 cut(s) 133, 176, 439, 685, 742, 1393, 1519, 1624, 1901
Hpy166II GTNNAC 5 cut(s) 141, 328, 1038, 1741, 1931
Hpy188I TCNGA 8 cut(s) 247, 423, 648, 748, 1263, 1281, 1315, 1437
Hpy8I GTNNAC 5 cut(s) 141, 328, 1038, 1741, 1931
Hpy99I CGWCG 9 cut(s) 251, 256, 326, 376, 907, 1031, 1042, 1285, 1748
HpyCH4III ACNGT 5 cut(s) 208, 427, 448, 1252, 1675
HpyCH4IV ACGT 4 cut(s) 132, 516, 600, 1479
HpyCH4V TGCA 5 cut(s) 259, 1069, 1183, 1827, 1841
HpyF10VI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1189, 1548, 1637, 1819, 1865
HpyF3I CTNAG 5 cut(s) 471, 806, 1214, 1434, 1547
HpySE526I ACGT 4 cut(s) 132, 516, 600, 1479
Hsp92I GRCGYC 1 cut(s) 578
Hsp92II CATG 7 cut(s) 271, 523, 940, 1016, 1666, 1882, 1900
HspAI GCGC 3 cut(s) 1134, 1866, 1908
KroI GCCGGC 3 cut(s) 1235, 1542, 1859
KroNI GCCGGC 3 cut(s) 1237, 1544, 1861
Ksp22I TGATCA 1 cut(s) 103
LmnI GCTCC 6 cut(s) 592, 961, 1090, 1423, 1810, 1925
Lsp1109I GCAGC 5 cut(s) 44, 157, 1072, 1179, 1182
LweI GCATC 3 cut(s) 42, 1450, 1570
MaeII ACGT 4 cut(s) 132, 516, 600, 1479
MaeIII GTNAC 3 cut(s) 164, 442, 1204
MbiI CCGCTC 4 cut(s) 154, 823, 1161, 1357
MflI RGATCY 3 cut(s) 1044, 1296, 1470
MhlI GDGCHC 4 cut(s) 302, 513, 1095, 1401
MluCI AATT 6 cut(s) 867, 1391, 1648, 1688, 1700, 1753
MluI ACGCGT 1 cut(s) 249
MlyI GAGTC 3 cut(s) 93, 461, 829
MmeI TCCRAC 5 cut(s) 270, 726, 1304, 1623, 1773
MroNI GCCGGC 3 cut(s) 1235, 1542, 1859
MroXI GAANNNNTTC 3 cut(s) 350, 737, 1020
MseI TTAA 3 cut(s) 285, 1080, 1752
MslI CAYNNNNRTG 2 cut(s) 702, 1883
MspA1I CMGCKG 2 cut(s) 57, 475
Mva1269I GAATGC 1 cut(s) 1642
MvaI CCWGG 4 cut(s) 790, 1423, 1853, 1902
MvnI CGCG 7 cut(s) 218, 251, 404, 902, 989, 1136, 1748
MwoI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1189, 1548, 1637, 1819, 1865
NaeI GCCGGC 3 cut(s) 1237, 1544, 1861
NciI CCSGG 9 cut(s) 297, 303, 508, 913, 914, 1326, 1696, 1709, 1935
NgoMIV GCCGGC 3 cut(s) 1235, 1542, 1859
NlaIII CATG 7 cut(s) 271, 523, 940, 1016, 1666, 1882, 1900
NmeAIII GCCGAG 2 cut(s) 21, 335
NmuCI GTSAC 3 cut(s) 164, 442, 1204
NspI RCATGY 2 cut(s) 1882, 1900
PaeI GCATGC 1 cut(s) 1882
PagI TCATGA 1 cut(s) 1012
PasI CCCWGGG 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 6 cut(s) 84, 647, 888, 993, 1046, 1277
PctI GAATGC 1 cut(s) 1642
PdiI GCCGGC 3 cut(s) 1237, 1544, 1861
PdmI GAANNNNTTC 3 cut(s) 350, 737, 1020
PfeI GAWTC 5 cut(s) 733, 872, 1258, 1336, 1407
PflFI GACNNNGTC 2 cut(s) 83, 838
Ple19I CGATCG 1 cut(s) 654
PleI GAGTC 3 cut(s) 92, 461, 829
PpsI GAGTC 3 cut(s) 92, 461, 829
Ppu21I YACGTR 1 cut(s) 133
PshBI ATTAAT 1 cut(s) 1752
Psp124BI GAGCTC 2 cut(s) 1095, 1401
Psp6I CCWGG 4 cut(s) 788, 1421, 1851, 1900
PspGI CCWGG 4 cut(s) 788, 1421, 1851, 1900
PspOMI GGGCCC 1 cut(s) 298
PspPI GGNCC 6 cut(s) 298, 299, 1540, 1705, 1931, 1937
PsuI RGATCY 3 cut(s) 1044, 1296, 1470
PsyI GACNNNGTC 2 cut(s) 83, 838
PvuI CGATCG 1 cut(s) 654
PvuII CAGCTG 1 cut(s) 57
RsaI GTAC 6 cut(s) 881, 1143, 1575, 1592, 1658, 1850
RsaNI GTAC 6 cut(s) 880, 1142, 1574, 1591, 1657, 1849
RseI CAYNNNNRTG 2 cut(s) 702, 1883
SacI GAGCTC 2 cut(s) 1095, 1401
SalI GTCGAC 1 cut(s) 1036
SaqAI TTAA 3 cut(s) 285, 1080, 1752
Sau96I GGNCC 6 cut(s) 298, 299, 1540, 1705, 1931, 1937
SchI GAGTC 3 cut(s) 93, 461, 829
SduI GDGCHC 4 cut(s) 302, 513, 1095, 1401
SfaNI GCATC 3 cut(s) 42, 1450, 1570
SinI GGWCC 2 cut(s) 1705, 1931
SmaI CCCGGG 1 cut(s) 914
SmiMI CAYNNNNRTG 2 cut(s) 702, 1883
SphI GCATGC 1 cut(s) 1882
Sse9I AATT 6 cut(s) 867, 1391, 1648, 1688, 1700, 1753
SstI GAGCTC 2 cut(s) 1095, 1401
StyI CCWWGG 2 cut(s) 486, 616
TaaI ACNGT 5 cut(s) 208, 427, 448, 1252, 1675
TaiI ACGT 4 cut(s) 135, 519, 603, 1482
TaqII GACCGA 2 cut(s) 29, 96
TasI AATT 6 cut(s) 867, 1391, 1648, 1688, 1700, 1753
TatI WGTACW 1 cut(s) 1590
TfiI GAWTC 5 cut(s) 733, 872, 1258, 1336, 1407
Tru1I TTAA 3 cut(s) 285, 1080, 1752
Tru9I TTAA 3 cut(s) 285, 1080, 1752
TscAI CASTG 2 cut(s) 1921, 1933
TseFI GTSAC 3 cut(s) 164, 442, 1204
TseI GCWGC 5 cut(s) 57, 170, 1060, 1192, 1195
Tsp45I GTSAC 3 cut(s) 164, 442, 1204
TspDTI ATGAA 6 cut(s) 206, 284, 947, 1001, 1029, 1362
TspMI CCCGGG 1 cut(s) 912
TspRI CASTG 2 cut(s) 1921, 1933
Tth111I GACNNNGTC 2 cut(s) 83, 838
VpaK11BI GGWCC 2 cut(s) 1705, 1931
VspI ATTAAT 1 cut(s) 1752
XapI RAATTY 2 cut(s) 1391, 1688
XceI RCATGY 2 cut(s) 1882, 1900
XmaI CCCGGG 1 cut(s) 912
XmiI GTMKAC 1 cut(s) 1037
XmnI GAANNNNTTC 3 cut(s) 350, 737, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.