Prupe.7G265200_v2.0.a1
HSP70 Family

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0008186
Physical Location & Seq
Forward (+)
21893033 .. 21895488
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G265200.1

Sequence Viewer

Length: 1965 bp
ATGGCGACCAAGACTGAAGGCAAGGCCATAGGCATCGACCTTGGCACAACCTATAGCTGCGTCGGTGTGTGGCAAAACGACCGCGTTGAGATCATCGCCAACGACCAGGGCAACCGCACCACTCCCTCTTACGTGGCCTTCACAGACACCGAGCGGCTCATCGGCGATGCAGCCAAAAACCAAGTGGCCATGAATCCACAGAACACCGTCTTCGACGCCAAGCGTCTCATTGGTCGGCGATTCTCCGACCCATCAGTCCAAAGCGACATGAAGCTCTGGCCTTTCAAGGTCATCCCTGGTCCCGGCGACAAACCCATGATCGTCGTCAGGTACAAAGGCGAAGAAAAGACCTTCTCTGCGGAGGAGATATCTTCCATGGTGCTGACGAAGATGAGGGAGATAGCTGAGGCTTATTTGGGTCAGACCATAAAGAACGCTGTGGTGACTGTCCCTGCTTATTTCAACGACTCCCAGAGACAGGCTACCAAGGACGCTGGCGCTATTTCTGGGCTCAACGTCATGCGAATTATCAACGAGCCCACCGCCGCCGCTATAGCCTATGGGCTGGACAAGAAGGCCTCGAGAAAAGGTGAGCAGAACGTCCTTATTTTCGATCTCGGCGGAGGCACTTTTGATGTCTCGCTTTTGACTATTGAAGAGGGGATATTTGAGGTGAAGGCCACCGCCGGCGATACTCATCTGGGTGGTGAAGATTTTGATAATAGAATGGTGAATCACTTTGTGGCCGAGTTCAGGAGGAAGCATAAGAAGGATATCAGTGGAAATGCCAGAGCTTTGAGGAGGTTGAGGACGGCGTGTGAGAGGGCTAAGAGGACCCTGTCTTCGACCTCTCAGACCACAATTGAGATTGATTCTTTGTACGAAGGGATCGATTTCTATGCGACGATTACAAGGGCCAGGTTCGAGGAGTTGAATATGGATTTGTTCAGGAAGTGTATGGAGCCGGTTGAAAAGTGTCTGAGGGATGCTAAGATTGACAAGAGTCAGGTTCACGACGTGGTTCTGGTTGGTGGGTCGACTAGAATTCCCAAGGTTCAGCAACTTTTGCAGGATTTTTTCAATGGGAAAGAGCTGTGCAAGAGCATAAACCCTGACGAAGCGGTTGCCTATGGTGCTGCGGTTCAAGCTGCGATCTTGAGCGGCGAAGGAGATGAGAAAGTGCAGGACCTGTTGTTGCTCGATGTCACGCCTCTCAGTCTTGGGCTTGAGACTGCTGGTGGTGTTATGACTACTCTCATCCCGAGGAACACAACAATTCCAACCAAGAAGGAGCAGATTTTCTCTACTTACTCCGATAATCAGCCCGGGGTTTTGATCCAAGTCTACGAAGGAGAGCGCGCCCGAACCAAGGACAACAATTTATTGGGGAAGTTTGAGCTCACTGGGATTCCTCCTGCTCCTAGAGGCGTGCCCCAAATCAATGTGTGCTTTGACATTGATGCGAATGGCATTTTGAATGTGTCTGCTGAGGACAAGACTGCTGGAGTGAAGAACAAGATCACCATCACCAACGACAAGGGAAGGCTGAGCAAGGAAGAAATAGAGAAGATGGTGCAAGATGCAGAGAAGTACAAGGCAGAGGACGAGGAGGTGAAGAAGAAAGTGGACGCCAAGAATGCGTTGGAAAACTACGCATATAACATGAGGAACACGGTGAAGGACGACAAGTTTGCAGGGAAACTGGACCCTGCGGACAAGGAGAAGATCGAGAAGGCAGTGGAGGATGCCATTAATTGGCTGGAGGGGAACCAGTTGGCTGAGGTTGATGAGTTGGAGGATAAGTTGAAGGAGTTGGAGGGGTTATGCAATCCAATTATTGCGAAGATGTACCAGGGCGGTGGTGGTGCTGGAGGCATGCCGCCTATGGGGGATGATGACATGTCTGGTGGTGCTTCTACTGGCTCTGCGACTGGACCTGGCCCTAAGATTGAAGAAGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

655

Amino Acids

71.63

Weight (kDa)

5.15

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 1755
AccBSI CCGCTC 2 cut(s) 154, 1161
AccI GTMKAC 2 cut(s) 1037, 1344
AccII CGCG 2 cut(s) 84, 1359
AclWI GGATC 2 cut(s) 896, 1330
AcoI YGGCCR 2 cut(s) 186, 744
AcsI RAATTY 1 cut(s) 1044
AcuI CTGAAG 1 cut(s) 36
AcyI GRCGYC 2 cut(s) 216, 1629
AdeI CACNNNGTG 2 cut(s) 742, 1018
AfaI GTAC 4 cut(s) 332, 881, 1592, 1850
AfiI CCNNNNNNNGG 6 cut(s) 302, 478, 753, 1369, 1755, 1886
AflIII ACRYGT 1 cut(s) 1899
AjiI CACGTC 1 cut(s) 1018
AjnI CCWGG 5 cut(s) 105, 295, 917, 1851, 1936
AluBI AGCT 7 cut(s) 57, 274, 404, 794, 1093, 1148, 1399
AluI AGCT 7 cut(s) 57, 274, 404, 794, 1093, 1148, 1399
Alw21I GWGCWC 1 cut(s) 1401
Alw26I GTCTC 4 cut(s) 230, 469, 643, 1223
AlwI GGATC 2 cut(s) 896, 1330
AlwNI CAGNNNCTG 1 cut(s) 1189
Ama87I CYCGRG 3 cut(s) 580, 1261, 1325
AoxI GGCC 9 cut(s) 24, 135, 186, 278, 576, 678, 744, 915, 1939
ApeKI GCWGC 4 cut(s) 57, 170, 1136, 1148
ApoI RAATTY 1 cut(s) 1044
ArsI GACNNNNNNTTYG 4 cut(s) 826, 858, 1673, 1705
AseI ATTAAT 1 cut(s) 1752
AspLEI GCGC 3 cut(s) 500, 1359, 1361
AspS9I GGNCC 7 cut(s) 299, 834, 915, 1186, 1705, 1934, 1940
AsuC2I CCSGG 3 cut(s) 303, 1326, 1327
AsuHPI GGTGA 9 cut(s) 454, 602, 685, 719, 742, 1513, 1519, 1624, 1687
AvaI CYCGRG 3 cut(s) 580, 1261, 1325
AvaII GGWCC 5 cut(s) 299, 834, 1186, 1705, 1934
BaeGI GKGCMC 1 cut(s) 1434
BalI TGGCCA 1 cut(s) 188
BanII GRGCYC 3 cut(s) 513, 540, 1401
BbsI GAAGAC 2 cut(s) 202, 834
Bbv12I GWGCWC 1 cut(s) 1401
BbvCI CCTCAGC 3 cut(s) 405, 1488, 1779
BbvI GCAGC 4 cut(s) 44, 182, 1123, 1135
BccI CCATC 3 cut(s) 259, 1532, 1564
BceAI ACGGC 1 cut(s) 828
BcgI CGANNNNNNTGC 6 cut(s) 881, 915, 1106, 1140, 1673, 1707
BciT130I CCWGG 5 cut(s) 107, 297, 919, 1853, 1938
BcnI CCSGG 3 cut(s) 303, 1326, 1327
BcoDI GTCTC 4 cut(s) 230, 469, 643, 1223
BfaI CTAG 2 cut(s) 1041, 1422
BfmI CTRYAG 2 cut(s) 52, 552
BfoI RGCGCY 1 cut(s) 501
BisI GCNGC 9 cut(s) 58, 155, 171, 546, 549, 1137, 1149, 1162, 1880
BlpI GCTNAGC 1 cut(s) 1547
BlsI GCNGC 9 cut(s) 59, 156, 172, 547, 550, 1138, 1150, 1163, 1881
Bme1390I CCNGG 8 cut(s) 107, 297, 303, 919, 1326, 1327, 1853, 1938
Bme18I GGWCC 5 cut(s) 299, 834, 1186, 1705, 1934
BmeT110I CYCGRG 3 cut(s) 580, 1261, 1325
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 7 cut(s) 299, 834, 915, 1186, 1705, 1934, 1940
BmiI GGNNCC 5 cut(s) 301, 836, 963, 1707, 1769
BmrFI CCNGG 8 cut(s) 107, 297, 303, 919, 1326, 1327, 1853, 1938
BmrI ACTGGG 1 cut(s) 1413
BmsI GCATC 6 cut(s) 42, 157, 976, 1450, 1570, 1735
BmuI ACTGGG 1 cut(s) 1413
BoxI GACNNNNGTC 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 202, 834
BpmI CTGGAG 3 cut(s) 1524, 1781, 1890
Bpu10I CCTNAGC 3 cut(s) 405, 1488, 1779
Bpu1102I GCTNAGC 1 cut(s) 1547
BpuEI CTTGAG 2 cut(s) 1177, 1247
BpuMI CCSGG 3 cut(s) 303, 1326, 1327
Bsa29I ATCGAT 1 cut(s) 891
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 696, 1523
BsaHI GRCGYC 2 cut(s) 216, 1629
BsaXI ACNNNNNCTCC 2 cut(s) 1787, 1817
Bsc4I CCNNNNNNNGG 6 cut(s) 302, 478, 753, 1369, 1755, 1886
Bse118I RCCGGY 2 cut(s) 686, 964
Bse1I ACTGG 5 cut(s) 1408, 1707, 1771, 1924, 1936
Bse8I GATNNNNATC 2 cut(s) 696, 1523
BseBI CCWGG 5 cut(s) 107, 297, 919, 1853, 1938
BseCI ATCGAT 1 cut(s) 891
BseGI GGATG 5 cut(s) 291, 991, 1257, 1750, 1897
BseJI GATNNNNATC 2 cut(s) 696, 1523
BseLI CCNNNNNNNGG 6 cut(s) 302, 478, 753, 1369, 1755, 1886
BseMII CTCAG 7 cut(s) 396, 866, 971, 1228, 1479, 1538, 1770
BseNI ACTGG 5 cut(s) 1408, 1707, 1771, 1924, 1936
BsePI GCGCGC 1 cut(s) 1357
BseRI GAGGAG 4 cut(s) 377, 814, 941, 1622
BseSI GKGCMC 1 cut(s) 1434
BseXI GCAGC 4 cut(s) 44, 182, 1123, 1135
BsgI GTGCAG 1 cut(s) 1202
Bsh1236I CGCG 2 cut(s) 84, 1359
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 9 cut(s) 26, 137, 188, 280, 578, 680, 746, 917, 1941
BshVI ATCGAT 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 1401
BsiHKCI CYCGRG 3 cut(s) 580, 1261, 1325
BsiSI CCGG 4 cut(s) 303, 687, 965, 1326
BslFI GGGAC 2 cut(s) 285, 434
BslI CCNNNNNNNGG 6 cut(s) 302, 478, 753, 1369, 1755, 1886
BsmAI GTCTC 4 cut(s) 230, 469, 643, 1223
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 285, 434
BsmI GAATGC 1 cut(s) 1642
BsnI GGCC 9 cut(s) 26, 137, 188, 280, 578, 680, 746, 917, 1941
BsoBI CYCGRG 3 cut(s) 580, 1261, 1325
Bsp1286I GDGCHC 4 cut(s) 513, 540, 1401, 1434
Bsp143I GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
Bsp1720I GCTNAGC 1 cut(s) 1547
Bsp19I CCATGG 1 cut(s) 375
BspANI GGCC 9 cut(s) 26, 137, 188, 280, 578, 680, 746, 917, 1941
BspCNI CTCAG 7 cut(s) 397, 865, 972, 1227, 1480, 1539, 1771
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 2 cut(s) 84, 1359
BspLI GGNNCC 5 cut(s) 301, 836, 963, 1707, 1769
BspPI GGATC 2 cut(s) 896, 1330
BsrBI CCGCTC 2 cut(s) 154, 1161
BsrFI RCCGGY 2 cut(s) 686, 964
BsrI ACTGG 5 cut(s) 1408, 1707, 1771, 1924, 1936
BssAI RCCGGY 2 cut(s) 686, 964
BssHII GCGCGC 1 cut(s) 1357
BssMI GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
BssNI GRCGYC 2 cut(s) 216, 1629
BssT1I CCWWGG 5 cut(s) 40, 375, 486, 1050, 1368
Bst2UI CCWGG 5 cut(s) 107, 297, 919, 1853, 1938
Bst4CI ACNGT 3 cut(s) 208, 448, 1675
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 2 cut(s) 216, 1629
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 5 cut(s) 496, 688, 1359, 1430, 1877
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 5 cut(s) 291, 991, 1257, 1750, 1897
BstFNI CGCG 2 cut(s) 84, 1359
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 3 cut(s) 500, 1359, 1361
BstKTI GATC 8 cut(s) 93, 321, 616, 891, 1155, 1338, 1521, 1728
BstMAI GTCTC 4 cut(s) 230, 469, 643, 1223
BstMBI GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 554, 1066, 1133, 1145, 1637
BstNI CCWGG 5 cut(s) 107, 297, 919, 1853, 1938
BstNSI RCATGY 2 cut(s) 1879, 1903
BstPAI GACNNNNGTC 1 cut(s) 1002
BstSCI CCNGG 8 cut(s) 105, 295, 301, 917, 1324, 1325, 1851, 1936
BstSFI CTRYAG 2 cut(s) 52, 552
BstSLI GKGCMC 1 cut(s) 1434
BstUI CGCG 2 cut(s) 84, 1359
BstV1I GCAGC 4 cut(s) 44, 182, 1123, 1135
BstV2I GAAGAC 2 cut(s) 202, 834
BstXI CCANNNNNNTGG 1 cut(s) 1859
Bsu15I ATCGAT 1 cut(s) 891
BsuRI GGCC 9 cut(s) 26, 137, 188, 280, 578, 680, 746, 917, 1941
BsuTUI ATCGAT 1 cut(s) 891
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 2 cut(s) 79, 180
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 5 cut(s) 291, 991, 1257, 1750, 1897
BtsI GCAGTG 1 cut(s) 1743
BtsIMutI CAGTG 3 cut(s) 784, 1401, 1743
Cac8I GCNNGC 5 cut(s) 496, 688, 1359, 1430, 1877
CaiI CAGNNNCTG 1 cut(s) 1189
CfoI GCGC 3 cut(s) 500, 1359, 1361
Cfr10I RCCGGY 2 cut(s) 686, 964
Cfr13I GGNCC 7 cut(s) 299, 834, 915, 1186, 1705, 1934, 1940
Cfr9I CCCGGG 1 cut(s) 1325
ClaI ATCGAT 1 cut(s) 891
CseI GACGC 5 cut(s) 49, 212, 224, 500, 1637
Csp6I GTAC 4 cut(s) 331, 880, 1591, 1849
CviAII CATG 8 cut(s) 190, 268, 316, 376, 520, 1663, 1876, 1900
CviQI GTAC 4 cut(s) 331, 880, 1591, 1849
DpnI GATC 8 cut(s) 92, 320, 615, 890, 1154, 1337, 1520, 1727
DpnII GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
DraIII CACNNNGTG 2 cut(s) 742, 1018
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 2 cut(s) 186, 744
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 1399
Eco130I CCWWGG 5 cut(s) 40, 375, 486, 1050, 1368
Eco147I AGGCCT 1 cut(s) 578
Eco24I GRGCYC 3 cut(s) 513, 540, 1401
Eco32I GATATC 2 cut(s) 369, 775
Eco47I GGWCC 5 cut(s) 299, 834, 1186, 1705, 1934
Eco53kI GAGCTC 1 cut(s) 1399
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 3 cut(s) 580, 1261, 1325
EcoICRI GAGCTC 1 cut(s) 1399
EcoO109I RGGNCCY 2 cut(s) 834, 1186
EcoRI GAATTC 1 cut(s) 1044
EcoRII CCWGG 5 cut(s) 105, 295, 917, 1851, 1936
EcoRV GATATC 2 cut(s) 369, 775
EcoT14I CCWWGG 5 cut(s) 40, 375, 486, 1050, 1368
EcoT38I GRGCYC 3 cut(s) 513, 540, 1401
ErhI CCWWGG 5 cut(s) 40, 375, 486, 1050, 1368
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 8 cut(s) 193, 271, 319, 379, 523, 1666, 1879, 1903
FaqI GGGAC 2 cut(s) 285, 434
FatI CATG 8 cut(s) 189, 267, 315, 375, 519, 1662, 1875, 1899
FblI GTMKAC 2 cut(s) 1037, 1344
Fnu4HI GCNGC 9 cut(s) 58, 155, 171, 546, 549, 1137, 1149, 1162, 1880
FokI GGATG 5 cut(s) 278, 998, 1244, 1757, 1904
FriOI GRGCYC 3 cut(s) 513, 540, 1401
Fsp4HI GCNGC 9 cut(s) 58, 155, 171, 546, 549, 1137, 1149, 1162, 1880
FspBI CTAG 2 cut(s) 1041, 1422
GlaI GCGC 3 cut(s) 499, 1358, 1360
GluI GCNGC 9 cut(s) 58, 155, 171, 546, 549, 1137, 1149, 1162, 1880
GsuI CTGGAG 3 cut(s) 1524, 1781, 1890
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 9 cut(s) 26, 137, 188, 280, 578, 680, 746, 917, 1941
HapII CCGG 4 cut(s) 303, 687, 965, 1326
HgaI GACGC 5 cut(s) 49, 212, 224, 500, 1637
HhaI GCGC 3 cut(s) 500, 1359, 1361
Hin1I GRCGYC 2 cut(s) 216, 1629
Hin1II CATG 8 cut(s) 193, 271, 319, 379, 523, 1666, 1879, 1903
Hin6I GCGC 3 cut(s) 498, 1357, 1359
HinP1I GCGC 3 cut(s) 498, 1357, 1359
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 7 cut(s) 193, 240, 467, 733, 872, 1003, 1408
HpaII CCGG 4 cut(s) 303, 687, 965, 1326
HphI GGTGA 9 cut(s) 454, 602, 685, 719, 742, 1513, 1519, 1624, 1687
Hpy166II GTNNAC 4 cut(s) 1012, 1038, 1345, 1627
Hpy188I TCNGA 5 cut(s) 247, 423, 855, 981, 1315
Hpy188III TCNNGA 7 cut(s) 582, 754, 949, 1013, 1156, 1261, 1729
Hpy8I GTNNAC 4 cut(s) 1012, 1038, 1345, 1627
Hpy99I CGWCG 5 cut(s) 65, 218, 326, 907, 1019
HpyCH4III ACNGT 3 cut(s) 208, 448, 1675
HpyCH4IV ACGT 4 cut(s) 132, 516, 600, 1017
HpyCH4V TGCA 8 cut(s) 170, 1069, 1098, 1183, 1576, 1583, 1694, 1827
HpyF10VI GCNNNNNNNGC 5 cut(s) 554, 1066, 1133, 1145, 1637
HpySE526I ACGT 4 cut(s) 132, 516, 600, 1017
Hsp92I GRCGYC 2 cut(s) 216, 1629
Hsp92II CATG 8 cut(s) 193, 271, 319, 379, 523, 1666, 1879, 1903
HspAI GCGC 3 cut(s) 498, 1357, 1359
KroI GCCGGC 1 cut(s) 686
KroNI GCCGGC 1 cut(s) 688
Kzo9I GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
LmnI GCTCC 3 cut(s) 961, 1291, 1423
Lsp1109I GCAGC 4 cut(s) 44, 182, 1123, 1135
LweI GCATC 6 cut(s) 42, 157, 976, 1450, 1570, 1735
MaeI CTAG 2 cut(s) 1041, 1422
MaeII ACGT 4 cut(s) 132, 516, 600, 1017
MaeIII GTNAC 2 cut(s) 442, 1204
MalI GATC 8 cut(s) 92, 320, 615, 890, 1154, 1337, 1520, 1727
MbiI CCGCTC 2 cut(s) 154, 1161
MboI GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
MfeI CAATTG 1 cut(s) 861
MhlI GDGCHC 4 cut(s) 513, 540, 1401, 1434
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 7 cut(s) 525, 861, 1044, 1275, 1378, 1753, 1833
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 461, 1012
MmeI TCCRAC 5 cut(s) 270, 1304, 1623, 1773, 1794
Mox20I TGGCCA 1 cut(s) 188
MreI CGCCGGCG 1 cut(s) 686
MroNI GCCGGC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 1752, 1963
MslI CAYNNNNRTG 1 cut(s) 702
Msp20I TGGCCA 1 cut(s) 188
MspI CCGG 4 cut(s) 303, 687, 965, 1326
MspR9I CCNGG 8 cut(s) 107, 297, 303, 919, 1326, 1327, 1853, 1938
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 1642
MvaI CCWGG 5 cut(s) 107, 297, 919, 1853, 1938
MvnI CGCG 2 cut(s) 84, 1359
MwoI GCNNNNNNNGC 5 cut(s) 554, 1066, 1133, 1145, 1637
NaeI GCCGGC 1 cut(s) 688
NciI CCSGG 3 cut(s) 303, 1326, 1327
NcoI CCATGG 1 cut(s) 375
NdeII GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
NgoMIV GCCGGC 1 cut(s) 686
NlaIII CATG 8 cut(s) 193, 271, 319, 379, 523, 1666, 1879, 1903
NlaIV GGNNCC 5 cut(s) 301, 836, 963, 1707, 1769
NmeAIII GCCGAG 2 cut(s) 597, 772
NmuCI GTSAC 2 cut(s) 442, 1204
NspI RCATGY 2 cut(s) 1879, 1903
PaeI GCATGC 1 cut(s) 1879
PaeR7I CTCGAG 1 cut(s) 580
PauI GCGCGC 1 cut(s) 1357
PceI AGGCCT 1 cut(s) 578
PciI ACATGT 1 cut(s) 1899
PcsI WCGNNNNNNNCGW 1 cut(s) 888
PctI GAATGC 1 cut(s) 1642
PdiI GCCGGC 1 cut(s) 688
PfeI GAWTC 5 cut(s) 193, 240, 733, 872, 1408
PflFI GACNNNGTC 1 cut(s) 838
PflMI CCANNNNNTGG 1 cut(s) 1755
PkrI GCNGC 9 cut(s) 59, 156, 172, 547, 550, 1138, 1150, 1163, 1881
PleI GAGTC 2 cut(s) 461, 1011
PpsI GAGTC 2 cut(s) 461, 1011
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 2 cut(s) 834, 1186
PscI ACATGT 1 cut(s) 1899
PshAI GACNNNNGTC 1 cut(s) 1002
PshBI ATTAAT 1 cut(s) 1752
Psp124BI GAGCTC 1 cut(s) 1401
Psp5II RGGWCCY 2 cut(s) 834, 1186
Psp6I CCWGG 5 cut(s) 105, 295, 917, 1851, 1936
PspGI CCWGG 5 cut(s) 105, 295, 917, 1851, 1936
PspN4I GGNNCC 5 cut(s) 301, 836, 963, 1707, 1769
PspPI GGNCC 7 cut(s) 299, 834, 915, 1186, 1705, 1934, 1940
PspPPI RGGWCCY 2 cut(s) 834, 1186
PstNI CAGNNNCTG 1 cut(s) 1189
PsyI GACNNNGTC 1 cut(s) 838
PteI GCGCGC 1 cut(s) 1357
RsaI GTAC 4 cut(s) 332, 881, 1592, 1850
RsaNI GTAC 4 cut(s) 331, 880, 1591, 1849
RseI CAYNNNNRTG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 1401
SalI GTCGAC 1 cut(s) 1036
SaqAI TTAA 2 cut(s) 1752, 1963
SatI GCNGC 9 cut(s) 58, 155, 171, 546, 549, 1137, 1149, 1162, 1880
Sau3AI GATC 8 cut(s) 90, 318, 613, 888, 1152, 1335, 1518, 1725
Sau96I GGNCC 7 cut(s) 299, 834, 915, 1186, 1705, 1934, 1940
SchI GAGTC 2 cut(s) 461, 1012
ScrFI CCNGG 8 cut(s) 107, 297, 303, 919, 1326, 1327, 1853, 1938
SduI GDGCHC 4 cut(s) 513, 540, 1401, 1434
SfaNI GCATC 6 cut(s) 42, 157, 976, 1450, 1570, 1735
SfcI CTRYAG 2 cut(s) 52, 552
Sfr274I CTCGAG 1 cut(s) 580
SgrAI CRCCGGYG 1 cut(s) 686
SinI GGWCC 5 cut(s) 299, 834, 1186, 1705, 1934
SlaI CTCGAG 1 cut(s) 580
SmaI CCCGGG 1 cut(s) 1327
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 3 cut(s) 580, 1156, 1226
SmoI CTYRAG 3 cut(s) 580, 1156, 1226
SphI GCATGC 1 cut(s) 1879
Sse9I AATT 7 cut(s) 525, 861, 1044, 1275, 1378, 1753, 1833
SseBI AGGCCT 1 cut(s) 578
SspMI CTAG 2 cut(s) 1041, 1422
SstI GAGCTC 1 cut(s) 1401
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 8 cut(s) 105, 295, 301, 917, 1324, 1325, 1851, 1936
StyI CCWWGG 5 cut(s) 40, 375, 486, 1050, 1368
TaaI ACNGT 3 cut(s) 208, 448, 1675
TaiI ACGT 4 cut(s) 135, 519, 603, 1020
TasI AATT 7 cut(s) 525, 861, 1044, 1275, 1378, 1753, 1833
TatI WGTACW 1 cut(s) 1590
TauI GCSGC 5 cut(s) 157, 548, 551, 1164, 1882
TfiI GAWTC 5 cut(s) 193, 240, 733, 872, 1408
Tru1I TTAA 2 cut(s) 1752, 1963
Tru9I TTAA 2 cut(s) 1752, 1963
TscAI CASTG 3 cut(s) 784, 1408, 1743
TseFI GTSAC 2 cut(s) 442, 1204
TseI GCWGC 4 cut(s) 57, 170, 1136, 1148
Tsp45I GTSAC 2 cut(s) 442, 1204
TspDTI ATGAA 2 cut(s) 206, 284
TspMI CCCGGG 1 cut(s) 1325
TspRI CASTG 3 cut(s) 784, 1408, 1743
Tth111I GACNNNGTC 1 cut(s) 838
Van91I CCANNNNNTGG 1 cut(s) 1755
VpaK11BI GGWCC 5 cut(s) 299, 834, 1186, 1705, 1934
VspI ATTAAT 1 cut(s) 1752
XapI RAATTY 1 cut(s) 1044
XceI RCATGY 2 cut(s) 1879, 1903
XcmI CCANNNNNNNNNTGG 4 cut(s) 181, 1639, 1756, 1859
XhoI CTCGAG 1 cut(s) 580
XmaI CCCGGG 1 cut(s) 1325
XmiI GTMKAC 2 cut(s) 1037, 1344
XspI CTAG 2 cut(s) 1041, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.