Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008186

Physical Location & Seq

Genomic Coordinates Reverse (-)

27397764 .. 27402054

Gene Structure 4291 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1225700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGGCTTCAAAGACGCAAGGTAAGGCCATCGGCATCGACCTCGGTACCACCTACAGCTGCGTCGGGGTGTGGCAGAACGACCGCGTTGAGATCATAGCCAATGACCAGGGCAACAGAACCACCCCTTCCTATGTCGCCTTCACCGACACCGAGCGTTTGATCGGCGACGCAGCCAAGAACCAGGTCGCTATGAACCCCCAAAACACCGTTTTCGACGCCAAGAGACTCATCGGCAGGCGGTTCTCCGACCCTTCTGTCCAGAGCGACATGAAGCACTGGCCTTTCAAGGTCGTAGCTGGCCCTGGCGACAAGCCCATGATTGTGGTAAATTACAAAGGCGAAGAGAAGCAGTTTTCACCGGAAGAGATTTCGTCAATGGTGCTGATTAAGATGAGGGAGATTGCCGAGGCTTATTTGGGTCACCCTTGCAACAACGCTGTGGTCACTGTACCTGCTTACTTCAATGACTCGCAGCGGCAGGCCACAAAGGATGCTGGCTCCATTGCCGGGCTCAATGTGATGAGGATTATCAACGAGCCTACTGCTGCTGCCATTGCTTACGGTCTTGACAAGAAGGGATCCCGGAGTGGCGAGAAGAATGTTCTTATTTTCGATCTTGGTGGAGGGACTTTCGATGTTTCTTTGCTCACAATTGAGGAAGGCATCTTCGAAGTGAAGGCTACTGCTGGCGATACTCATCTTGGTGGTGAGGATTTTGACAACAGGCTTGTTAATCACTTTGTACAAGAGTTTAAGAGAAAGCACAAGAAGGATATTAGTAGCAATGCCAGAGCTTTGAGGAGGTTGAGGACTGCATGCGAGAGGGCGAAAAGGACTCTGTCTTCCACCTCTCAGACTACCATTGAGATCGATTCGCTCTACGAAGGTATTGATTTCTATTCTACAATTACTAGGGCGAGATTTGAAGAGATGAATATGGATTTGTTTAGGAAGTGTATGGAACCTGTTGAGAAGTGTTTGAGGGATTCCAAAATCGACAAGAGTCGGGTTGATGAGGTTGTTTTGGTTGGTGGGTCGACGAGAATTCCCAAAGTTCAGCAGCTTTTGCAAGATTTCTTCAATGGCAAGGAATTGTGCAAGAGCATAAACCCTGACGAAGCCGTGGCTTATGGTGCTGCTGTTCAAGCTGCAATTTTAACCGGCGAAGGAAATGAGAAGGTCCAAGATCTGTTGCTTCTTGATGTCACTCCTCTCAGCCTCGGTCTTGAGACTGCAGGTGGTAAGGTGGATGCCAAGAACTCACTCGAGAACTACGCCTACAATATGCGGAACACTGTCAAGGATGAGAAGGTTGCAGGAAAATTAGACCCTGCAGACAAGCAGAAGATTGAGAAGGCCATTGACGAGGCAATTGAGTGGCTAGACAGGAACCAGTTGGCCGAGGTGGAGGAGTTTGAGGACAAGCAAAAGGAGTTGGAGGGATTGTGCAACCCGATTATTGCCAAAATGTATCAAGGTGCTGGTGGGGATGTGCCGATGGGCGGTGGTGCTCAGATGCCTGGAGGTGGATTTGGAAACGCTAGCTCCGGTGGTGGATCAGGAGCCGGACCTAAGATTGAAGAGGTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169

Pfam Domains

Protein Families

HSP70

Protein Analysis

531

Amino Acids

57.87

Weight (kDa)

5.26

Isoelectric Point (pI)

38.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	9 - 416	9.4e-197	Hsp70 protein
MreB_Mbl	PF06723	143 - 380	1e-13	MreB/Mbl protein
HSP70	PF00012	416 - 492	3.2e-06	Hsp70 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008186)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16030
fragaria_vesca	FvH4_1g00710
malus_domestica	MD02G1005800.v1.1 MD15G1150500.v1.1 MD17G1225700.v1.1 MD17G1226000.v1.1 MD17G1226100.v1.1
prunus_persica	Prupe.3G102200_v2.0.a1 Prupe.3G102300_v2.0.a1 Prupe.7G265200_v2.0.a1
pyrus_communis	pycom15g13460 pycom17g22940
rosa_chinensis	RchiOBHm_Chr2g0085421
rosa_laevigata	RLG00000015686
rosa_multiflora	Rmu_sc0004926.1_g000018 Rmu_sc0007908.1_g000014
rosa_roxburghii	Rroxscaffold_2G00155390
rosa_rugosa	Rorug01G0460300 Rorug01G0460400 Rorug01G0460400
rosa_samantha	Rh2AG008500 Rh2CG010300
rosa_wichuraiana	Rw2G000820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1226
AasI	GACNNNNNNGTC	1 cut(s)	254
Acc36I	ACCTGC	2 cut(s)	460, 1226
Acc65I	GGTACC	1 cut(s)	44
AccB1I	GGYRCC	1 cut(s)	44
AccI	GTMKAC	1 cut(s)	1037
AccII	CGCG	1 cut(s)	84
AciI	CCGC	5 cut(s)	82, 238, 475, 1288, 1503
AclWI	GGATC	3 cut(s)	573, 586, 1564
AcoI	YGGCCR	1 cut(s)	1398
AcsI	RAATTY	1 cut(s)	1044
AcyI	GRCGYC	1 cut(s)	216
AfaI	GTAC	3 cut(s)	46, 450, 744
AfiI	CCNNNNNNNGG	3 cut(s)	507, 1502, 1526
AgsI	TTSAA	7 cut(s)	9, 286, 463, 926, 1081, 1145, 1580
AjnI	CCWGG	4 cut(s)	105, 180, 301, 1519
AluBI	AGCT	6 cut(s)	57, 296, 794, 1063, 1148, 1545
AluI	AGCT	6 cut(s)	57, 296, 794, 1063, 1148, 1545
Alw21I	GWGCWC	1 cut(s)	1513
Alw26I	GTCTC	2 cut(s)	217, 1223
AlwI	GGATC	3 cut(s)	573, 586, 1564
Ama87I	CYCGRG	1 cut(s)	1265
AoxI	GGCC	6 cut(s)	24, 278, 298, 480, 1356, 1398
ApeKI	GCWGC	8 cut(s)	57, 170, 472, 545, 548, 1060, 1136, 1148
ApoI	RAATTY	1 cut(s)	1044
Asp718I	GGTACC	1 cut(s)	44
AspS9I	GGNCC	3 cut(s)	299, 1180, 1568
AsuC2I	CCSGG	2 cut(s)	508, 583
AsuHPI	GGTGA	4 cut(s)	133, 348, 413, 719
AsuII	TTCGAA	1 cut(s)	669
AsuNHI	GCTAGC	1 cut(s)	1541
AvaI	CYCGRG	1 cut(s)	1265
AvaII	GGWCC	2 cut(s)	1180, 1568
BaeI	ACNNNNGTAYC	2 cut(s)	28, 61
BamHI	GGATCC	1 cut(s)	578
BanI	GGYRCC	1 cut(s)	44
BanII	GRGCYC	1 cut(s)	513
BbsI	GAAGAC	1 cut(s)	834
Bbv12I	GWGCWC	1 cut(s)	1513
BbvI	GCAGC	8 cut(s)	44, 182, 484, 532, 535, 1072, 1123, 1135
BccI	CCATC	2 cut(s)	35, 1492
BceAI	ACGGC	1 cut(s)	1106
BcgI	CGANNNNNNTGC	4 cut(s)	22, 56, 524, 558
BciT130I	CCWGG	4 cut(s)	107, 182, 303, 1521
BcnI	CCSGG	2 cut(s)	508, 583
BcoDI	GTCTC	2 cut(s)	217, 1223
BfaI	CTAG	3 cut(s)	912, 1382, 1542
BfmI	CTRYAG	3 cut(s)	52, 1233, 1332
BfuAI	ACCTGC	2 cut(s)	460, 1226
BglII	AGATCT	1 cut(s)	1186
BisI	GCNGC	9 cut(s)	58, 171, 473, 476, 546, 549, 1061, 1137, 1149
BlsI	GCNGC	9 cut(s)	59, 172, 474, 477, 547, 550, 1062, 1138, 1150
Bme1390I	CCNGG	6 cut(s)	107, 182, 303, 508, 583, 1521
Bme18I	GGWCC	2 cut(s)	1180, 1568
BmeT110I	CYCGRG	1 cut(s)	1265
BmgT120I	GGNCC	3 cut(s)	299, 1180, 1568
BmiI	GGNNCC	6 cut(s)	46, 499, 580, 963, 1391, 1564
BmrFI	CCNGG	6 cut(s)	107, 182, 303, 508, 583, 1521
BmsI	GCATC	5 cut(s)	42, 481, 672, 1240, 1506
BmtI	GCTAGC	1 cut(s)	1545
BoxI	GACNNNNGTC	1 cut(s)	1002
BpiI	GAAGAC	1 cut(s)	834
BpmI	CTGGAG	1 cut(s)	1542
Bpu14I	TTCGAA	1 cut(s)	669
BpuEI	CTTGAG	1 cut(s)	1247
BpuMI	CCSGG	2 cut(s)	508, 583
Bsa29I	ATCGAT	1 cut(s)	870
BsaBI	GATNNNNATC	1 cut(s)	696
BsaHI	GRCGYC	1 cut(s)	216
BsaJI	CCNNGG	7 cut(s)	40, 106, 301, 405, 1122, 1219, 1401
BsaWI	WCCGGW	2 cut(s)	358, 1547
BsaXI	ACNNNNNCTCC	4 cut(s)	1430, 1460, 1529, 1559
Bsc4I	CCNNNNNNNGG	3 cut(s)	507, 1502, 1526
Bse118I	RCCGGY	1 cut(s)	1160
Bse1I	ACTGG	2 cut(s)	281, 1393
Bse3DI	GCAATG	3 cut(s)	501, 552, 790
Bse8I	GATNNNNATC	1 cut(s)	696
BseBI	CCWGG	4 cut(s)	107, 182, 303, 1521
BseCI	ATCGAT	1 cut(s)	870
BseDI	CCNNGG	7 cut(s)	40, 106, 301, 405, 1122, 1219, 1401
BseGI	GGATG	4 cut(s)	496, 1255, 1309, 1495
BseJI	GATNNNNATC	1 cut(s)	696
BseLI	CCNNNNNNNGG	3 cut(s)	507, 1502, 1526
BseMI	GCAATG	3 cut(s)	501, 552, 790
BseMII	CTCAG	3 cut(s)	866, 1228, 1526
BseNI	ACTGG	2 cut(s)	281, 1393
BseRI	GAGGAG	3 cut(s)	814, 1200, 1424
BseXI	GCAGC	8 cut(s)	44, 182, 484, 532, 535, 1072, 1123, 1135
Bsh1236I	CGCG	1 cut(s)	84
Bsh1285I	CGRYCG	1 cut(s)	82
BshFI	GGCC	6 cut(s)	26, 280, 300, 482, 1358, 1400
BshNI	GGYRCC	1 cut(s)	44
BshVI	ATCGAT	1 cut(s)	870
BsiEI	CGRYCG	1 cut(s)	82
BsiHKAI	GWGCWC	1 cut(s)	1513
BsiHKCI	CYCGRG	1 cut(s)	1265
BsiSI	CCGG	6 cut(s)	359, 507, 583, 1161, 1548, 1566
BslFI	GGGAC	1 cut(s)	640
BslI	CCNNNNNNNGG	3 cut(s)	507, 1502, 1526
BsmAI	GTCTC	2 cut(s)	217, 1223
BsmFI	GGGAC	1 cut(s)	640
BsnI	GGCC	6 cut(s)	26, 280, 300, 482, 1358, 1400
BsoBI	CYCGRG	1 cut(s)	1265
Bsp119I	TTCGAA	1 cut(s)	669
Bsp1286I	GDGCHC	2 cut(s)	513, 1513
Bsp1407I	TGTACA	1 cut(s)	742
Bsp143I	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
BspACI	CCGC	5 cut(s)	82, 238, 475, 1288, 1503
BspANI	GGCC	6 cut(s)	26, 280, 300, 482, 1358, 1400
BspCNI	CTCAG	3 cut(s)	865, 1227, 1525
BspDI	ATCGAT	1 cut(s)	870
BspFNI	CGCG	1 cut(s)	84
BspLI	GGNNCC	6 cut(s)	46, 499, 580, 963, 1391, 1564
BspMAI	CTGCAG	2 cut(s)	1237, 1336
BspMI	ACCTGC	2 cut(s)	460, 1226
BspOI	GCTAGC	1 cut(s)	1545
BspPI	GGATC	3 cut(s)	573, 586, 1564
BspT104I	TTCGAA	1 cut(s)	669
BspT107I	GGYRCC	1 cut(s)	44
BsrDI	GCAATG	3 cut(s)	501, 552, 790
BsrFI	RCCGGY	1 cut(s)	1160
BsrGI	TGTACA	1 cut(s)	742
BsrI	ACTGG	2 cut(s)	281, 1393
BssAI	RCCGGY	1 cut(s)	1160
BssECI	CCNNGG	7 cut(s)	40, 106, 301, 405, 1122, 1219, 1401
BssMI	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
BssNI	GRCGYC	1 cut(s)	216
Bst2UI	CCWGG	4 cut(s)	107, 182, 303, 1521
Bst4CI	ACNGT	4 cut(s)	208, 448, 563, 1297
Bst6I	CTCTTC	4 cut(s)	336, 357, 921, 1575
BstACI	GRCGYC	1 cut(s)	216
BstAPI	GCANNNNNTGC	1 cut(s)	1066
BstAUI	TGTACA	1 cut(s)	742
BstBI	TTCGAA	1 cut(s)	669
BstC8I	GCNNGC	7 cut(s)	236, 298, 480, 496, 688, 817, 1543
BstDEI	CTNAG	4 cut(s)	852, 1214, 1512, 1572
BstDSI	CCRYGG	1 cut(s)	1122
BstEII	GGTNACC	1 cut(s)	419
BstF5I	GGATG	4 cut(s)	496, 1255, 1309, 1495
BstFNI	CGCG	1 cut(s)	84
BstKTI	GATC	7 cut(s)	93, 162, 581, 616, 870, 1189, 1559
BstMAI	GTCTC	2 cut(s)	217, 1223
BstMBI	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
BstMCI	CGRYCG	1 cut(s)	82
BstMWI	GCNNNNNNNGC	4 cut(s)	554, 1066, 1133, 1145
BstNI	CCWGG	4 cut(s)	107, 182, 303, 1521
BstNSI	RCATGY	1 cut(s)	819
BstPAI	GACNNNNGTC	1 cut(s)	1002
BstPI	GGTNACC	1 cut(s)	419
BstSCI	CCNGG	6 cut(s)	105, 180, 301, 506, 581, 1519
BstSFI	CTRYAG	3 cut(s)	52, 1233, 1332
BstUI	CGCG	1 cut(s)	84
BstV1I	GCAGC	8 cut(s)	44, 182, 484, 532, 535, 1072, 1123, 1135
BstV2I	GAAGAC	1 cut(s)	834
BstX2I	RGATCY	2 cut(s)	578, 1186
BstXI	CCANNNNNNTGG	1 cut(s)	322
BstYI	RGATCY	2 cut(s)	578, 1186
Bsu15I	ATCGAT	1 cut(s)	870
BsuRI	GGCC	6 cut(s)	26, 280, 300, 482, 1358, 1400
BsuTUI	ATCGAT	1 cut(s)	870
BtgI	CCRYGG	1 cut(s)	1122
BtsCI	GGATG	4 cut(s)	496, 1255, 1309, 1495
BtsIMutI	CAGTG	3 cut(s)	274, 444, 1293
BveI	ACCTGC	2 cut(s)	460, 1226
Cac8I	GCNNGC	7 cut(s)	236, 298, 480, 496, 688, 817, 1543
Cfr10I	RCCGGY	1 cut(s)	1160
Cfr13I	GGNCC	3 cut(s)	299, 1180, 1568
ClaI	ATCGAT	1 cut(s)	870
CseI	GACGC	4 cut(s)	22, 49, 176, 224
CsiI	ACCWGGT	1 cut(s)	180
Csp6I	GTAC	3 cut(s)	45, 449, 743
CviAII	CATG	3 cut(s)	268, 316, 816
CviQI	GTAC	3 cut(s)	45, 449, 743
DdeI	CTNAG	4 cut(s)	852, 1214, 1512, 1572
DpnI	GATC	7 cut(s)	92, 161, 580, 615, 869, 1188, 1558
DpnII	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
DrdI	GACNNNNNNGTC	1 cut(s)	254
DseDI	GACNNNNNNGTC	1 cut(s)	254
EaeI	YGGCCR	1 cut(s)	1398
Eam1104I	CTCTTC	4 cut(s)	336, 357, 921, 1575
EarI	CTCTTC	4 cut(s)	336, 357, 921, 1575
Eco24I	GRGCYC	1 cut(s)	513
Eco47I	GGWCC	2 cut(s)	1180, 1568
Eco88I	CYCGRG	1 cut(s)	1265
Eco91I	GGTNACC	1 cut(s)	419
EcoO65I	GGTNACC	1 cut(s)	419
EcoRI	GAATTC	1 cut(s)	1044
EcoRII	CCWGG	4 cut(s)	105, 180, 301, 1519
EcoT38I	GRGCYC	1 cut(s)	513
FaeI	CATG	3 cut(s)	271, 319, 819
FaqI	GGGAC	1 cut(s)	640
FatI	CATG	3 cut(s)	267, 315, 815
FblI	GTMKAC	1 cut(s)	1037
Fnu4HI	GCNGC	9 cut(s)	58, 171, 473, 476, 546, 549, 1061, 1137, 1149
FokI	GGATG	4 cut(s)	503, 1262, 1316, 1502
FriOI	GRGCYC	1 cut(s)	513
Fsp4HI	GCNGC	9 cut(s)	58, 171, 473, 476, 546, 549, 1061, 1137, 1149
FspBI	CTAG	3 cut(s)	912, 1382, 1542
GluI	GCNGC	9 cut(s)	58, 171, 473, 476, 546, 549, 1061, 1137, 1149
GsuI	CTGGAG	1 cut(s)	1542
HaeIII	GGCC	6 cut(s)	26, 280, 300, 482, 1358, 1400
HapII	CCGG	6 cut(s)	359, 507, 583, 1161, 1548, 1566
HgaI	GACGC	4 cut(s)	22, 49, 176, 224
Hin1I	GRCGYC	1 cut(s)	216
Hin1II	CATG	3 cut(s)	271, 319, 819
HincII	GTYRAC	1 cut(s)	1038
HindII	GTYRAC	1 cut(s)	1038
HinfI	GANTC	6 cut(s)	225, 467, 835, 872, 986, 1003
HpaII	CCGG	6 cut(s)	359, 507, 583, 1161, 1548, 1566
HphI	GGTGA	4 cut(s)	133, 348, 413, 719
Hpy166II	GTNNAC	1 cut(s)	1038
Hpy188I	TCNGA	3 cut(s)	247, 855, 1515
Hpy188III	TCNNGA	6 cut(s)	259, 566, 1199, 1226, 1267, 1560
Hpy8I	GTNNAC	1 cut(s)	1038
Hpy99I	CGWCG	4 cut(s)	65, 170, 218, 1042
HpyCH4III	ACNGT	4 cut(s)	208, 448, 563, 1297
HpyCH4V	TGCA	9 cut(s)	429, 815, 1069, 1098, 1151, 1235, 1316, 1334, 1449
HpyF10VI	GCNNNNNNNGC	4 cut(s)	554, 1066, 1133, 1145
HpyF3I	CTNAG	4 cut(s)	852, 1214, 1512, 1572
Hsp92I	GRCGYC	1 cut(s)	216
Hsp92II	CATG	3 cut(s)	271, 319, 819
KpnI	GGTACC	1 cut(s)	48
Kzo9I	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
LmnI	GCTCC	3 cut(s)	503, 1550, 1562
Lsp1109I	GCAGC	8 cut(s)	44, 182, 484, 532, 535, 1072, 1123, 1135
LweI	GCATC	5 cut(s)	42, 481, 672, 1240, 1506
MabI	ACCWGGT	1 cut(s)	180
MaeI	CTAG	3 cut(s)	912, 1382, 1542
MaeIII	GTNAC	3 cut(s)	419, 442, 1204
MalI	GATC	7 cut(s)	92, 161, 580, 615, 869, 1188, 1558
MboI	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
MboII	GAAGA	9 cut(s)	353, 374, 607, 658, 834, 938, 1069, 1357, 1592
MfeI	CAATTG	2 cut(s)	651, 1371
MflI	RGATCY	2 cut(s)	578, 1186
MhlI	GDGCHC	2 cut(s)	513, 1513
MluCI	AATT	8 cut(s)	328, 651, 906, 1044, 1091, 1152, 1322, 1371
MlyI	GAGTC	4 cut(s)	219, 461, 829, 1012
MmeI	TCCRAC	2 cut(s)	270, 1416
MseI	TTAA	5 cut(s)	387, 732, 753, 1157, 1591
MslI	CAYNNNNRTG	2 cut(s)	320, 702
MspA1I	CMGCKG	2 cut(s)	57, 475
MspI	CCGG	6 cut(s)	359, 507, 583, 1161, 1548, 1566
MspR9I	CCNGG	6 cut(s)	107, 182, 303, 508, 583, 1521
MunI	CAATTG	2 cut(s)	651, 1371
MvaI	CCWGG	4 cut(s)	107, 182, 303, 1521
MvnI	CGCG	1 cut(s)	84
MwoI	GCNNNNNNNGC	4 cut(s)	554, 1066, 1133, 1145
NciI	CCSGG	2 cut(s)	508, 583
NdeII	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
NheI	GCTAGC	1 cut(s)	1541
NlaIII	CATG	3 cut(s)	271, 319, 819
NlaIV	GGNNCC	6 cut(s)	46, 499, 580, 963, 1391, 1564
NmeAIII	GCCGAG	2 cut(s)	430, 1426
NmuCI	GTSAC	3 cut(s)	419, 442, 1204
NspI	RCATGY	1 cut(s)	819
NspV	TTCGAA	1 cut(s)	669
PaeI	GCATGC	1 cut(s)	819
PaeR7I	CTCGAG	1 cut(s)	1265
PaqCI	CACCTGC	1 cut(s)	1226
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PfeI	GAWTC	2 cut(s)	872, 986
PflFI	GACNNNGTC	1 cut(s)	838
PfoI	TCCNGGA	1 cut(s)	581
PkrI	GCNGC	9 cut(s)	59, 172, 474, 477, 547, 550, 1062, 1138, 1150
PleI	GAGTC	4 cut(s)	219, 461, 829, 1011
PpsI	GAGTC	4 cut(s)	219, 461, 829, 1011
PshAI	GACNNNNGTC	1 cut(s)	1002
Psp6I	CCWGG	4 cut(s)	105, 180, 301, 1519
PspEI	GGTNACC	1 cut(s)	419
PspGI	CCWGG	4 cut(s)	105, 180, 301, 1519
PspN4I	GGNNCC	6 cut(s)	46, 499, 580, 963, 1391, 1564
PspPI	GGNCC	3 cut(s)	299, 1180, 1568
PsrI	GAACNNNNNNTAC	2 cut(s)	1262, 1294
PstI	CTGCAG	2 cut(s)	1237, 1336
PsuI	RGATCY	2 cut(s)	578, 1186
PsyI	GACNNNGTC	1 cut(s)	838
PvuII	CAGCTG	1 cut(s)	57
RsaI	GTAC	3 cut(s)	46, 450, 744
RsaNI	GTAC	3 cut(s)	45, 449, 743
RseI	CAYNNNNRTG	2 cut(s)	320, 702
SalI	GTCGAC	1 cut(s)	1036
SaqAI	TTAA	5 cut(s)	387, 732, 753, 1157, 1591
SatI	GCNGC	9 cut(s)	58, 171, 473, 476, 546, 549, 1061, 1137, 1149
Sau3AI	GATC	7 cut(s)	90, 159, 578, 613, 867, 1186, 1556
Sau96I	GGNCC	3 cut(s)	299, 1180, 1568
SchI	GAGTC	4 cut(s)	219, 461, 829, 1012
ScrFI	CCNGG	6 cut(s)	107, 182, 303, 508, 583, 1521
SduI	GDGCHC	2 cut(s)	513, 1513
SexAI	ACCWGGT	1 cut(s)	180
SfaNI	GCATC	5 cut(s)	42, 481, 672, 1240, 1506
SfcI	CTRYAG	3 cut(s)	52, 1233, 1332
Sfr274I	CTCGAG	1 cut(s)	1265
SfuI	TTCGAA	1 cut(s)	669
SinI	GGWCC	2 cut(s)	1180, 1568
SlaI	CTCGAG	1 cut(s)	1265
SmiMI	CAYNNNNRTG	2 cut(s)	320, 702
SmlI	CTYRAG	2 cut(s)	1226, 1265
SmoI	CTYRAG	2 cut(s)	1226, 1265
SphI	GCATGC	1 cut(s)	819
Sse9I	AATT	8 cut(s)	328, 651, 906, 1044, 1091, 1152, 1322, 1371
SsiI	CCGC	5 cut(s)	82, 238, 475, 1288, 1503
SspMI	CTAG	3 cut(s)	912, 1382, 1542
StyD4I	CCNGG	6 cut(s)	105, 180, 301, 506, 581, 1519
TaaI	ACNGT	4 cut(s)	208, 448, 563, 1297
TaqI	TCGA	9 cut(s)	36, 213, 612, 633, 669, 870, 996, 1037, 1266
TaqII	GACCGA	1 cut(s)	1211
TasI	AATT	8 cut(s)	328, 651, 906, 1044, 1091, 1152, 1322, 1371
TatI	WGTACW	1 cut(s)	742
TauI	GCSGC	1 cut(s)	478
TfiI	GAWTC	2 cut(s)	872, 986
Tru1I	TTAA	5 cut(s)	387, 732, 753, 1157, 1591
Tru9I	TTAA	5 cut(s)	387, 732, 753, 1157, 1591
TscAI	CASTG	3 cut(s)	281, 451, 1300
TseFI	GTSAC	3 cut(s)	419, 442, 1204
TseI	GCWGC	8 cut(s)	57, 170, 472, 545, 548, 1060, 1136, 1148
Tsp45I	GTSAC	3 cut(s)	419, 442, 1204
TspDTI	ATGAA	3 cut(s)	206, 284, 947
TspRI	CASTG	3 cut(s)	281, 451, 1300
Tth111I	GACNNNGTC	1 cut(s)	838
VpaK11BI	GGWCC	2 cut(s)	1180, 1568
XapI	RAATTY	1 cut(s)	1044
XceI	RCATGY	1 cut(s)	819
XhoI	CTCGAG	1 cut(s)	1265
XmiI	GTMKAC	1 cut(s)	1037
XspI	CTAG	3 cut(s)	912, 1382, 1542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.