pycom15g13460
HSP70 Family

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008186
Physical Location & Seq
Reverse (-)
9168225 .. 9169460
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13460.1

Sequence Viewer

Length: 1236 bp
ATGGTGAATCACTTCGTGGCGGAGTTCCGGAGGAAGCATAAGAAGGATATTAGCGGAAACGCCAGGGCGTTGCGGCGGCTGAGGACAGCATGCGAACGGGCGAAGAGGACTCTATCTTCCACCACCCAGACGGCAATCGAAATTGATTCCTTGTACGAAGGTATCGATTTCTACTCGACGATTACTCGCGCGAGGTTCGAGGAATTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCCGTCGAGAAATGTCTGAGGGATGCGAAGATGGACAAGTCGCAGATTCATGAGGTAGTTCTTGTCGGCGGGTCGACAAGAATCCCGAAAGTCCAGCAGCTTTTGCAGGACTTTTTTAACGGGAAGGAACTTTGTAAGAGTATAAACCCCGACGAGGCTGTCGCTTACGGCGCGGCGGTGCAAGCGGCGATCTTGACCGGCGAAGGGGATCAGAAAGTGCAGGACTTGCTGCTGCTCGATGTCACGCCGCTCAGTCTCGGTCTCGAGACCGCCGGCGGGGTTATGACTGTGCTGATTCCGAGGAACACGACAATTCCGACAAAGAAAGAGCAGATCTTTTCGACTTATTCGGATAATCAACCCGGAGTTTTGATCCAGGTTTATGAAGGAGAGAGAGCGCGAACGAAGGACAACAACTTGTTGGGGAAGTTTGAGCTCTCGGGCATCCCTCCGGCTCCGAGAGGCGTGCCGCAGATCAATGTGGTCTTCGACATTGATGCGAATGGGATTCTGAATGTGTCGGCTGAGGACAAGACGGCAGGGGTGAAGAACAAGATTACGATTACGAATGACAAGGGACGACTGAGCAAGGAGGAGATCGAGAAGATGGTGCAGGATGCGGAGAAGTACAGGGCGGAGGACGAGGAGGTGAAGAGGAAGGTGGATGCAAAGAATGCGTTGGAGAATTATGCGTACAACATGAGGAACACCGTGAGGGATGATAAATTTGCCGGGAAATTGGACCCCGCGGACAAGGAGAAGATCGAGAAGGCGGTGGACGAGGTGATCAATTGGCTGGAGGGGAACCAGTTGGCGGAAGTTGATGAGTTTGAGCACAAGTTGAAGGAGCTGGAGGGCCTGTGCAACCCTATTATTGCGAAGATGTACCAGGGTGCCGGCGGTGCTGGAGGCGGTATGCCGATGGGAGATGACATGCCTGGTGGCGCTTCCACTGGCTCCGCCAATGGACCCGGCCCCAAGATTGAGGAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

412

Amino Acids

45.38

Weight (kDa)

5.34

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 376 2.7e-137 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 1130
AccBSI CCGCTC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 311
AccII CGCG 5 cut(s) 189, 191, 410, 637, 986
AccIII TCCGGA 1 cut(s) 27
AclWI GGATC 2 cut(s) 453, 604
AcsI RAATTY 1 cut(s) 962
AdeI CACNNNGTG 1 cut(s) 16
AfaI GTAC 4 cut(s) 155, 866, 932, 1124
AfiI CCNNNNNNNGG 4 cut(s) 391, 441, 513, 1051
AgsI TTSAA 2 cut(s) 208, 1081
AjnI CCWGG 4 cut(s) 62, 612, 1125, 1174
AluBI AGCT 3 cut(s) 337, 673, 1087
AluI AGCT 3 cut(s) 337, 673, 1087
Alw21I GWGCWC 2 cut(s) 675, 1074
Alw26I GTCTC 3 cut(s) 497, 497, 503
AlwI GGATC 2 cut(s) 453, 604
Ama87I CYCGRG 2 cut(s) 500, 676
Aor13HI TCCGGA 1 cut(s) 27
AoxI GGCC 2 cut(s) 1093, 1210
ApeKI GCWGC 3 cut(s) 334, 466, 469
ApoI RAATTY 1 cut(s) 962
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 4 cut(s) 191, 410, 637, 1184
AspS9I GGNCC 4 cut(s) 979, 1093, 1205, 1211
AsuC2I CCSGG 3 cut(s) 600, 970, 1209
AsuHPI GGTGA 4 cut(s) 16, 793, 898, 1033
AvaI CYCGRG 2 cut(s) 500, 676
AvaII GGWCC 2 cut(s) 979, 1205
BaeI ACNNNNGTAYC 2 cut(s) 145, 178
BanI GGYRCC 1 cut(s) 1130
BanII GRGCYC 2 cut(s) 240, 675
BbsI GAAGAC 1 cut(s) 715
Bbv12I GWGCWC 2 cut(s) 675, 1074
BbvCI CCTCAGC 2 cut(s) 80, 762
BbvI GCAGC 3 cut(s) 346, 453, 456
BccI CCATC 3 cut(s) 262, 838, 1153
BceAI ACGGC 3 cut(s) 147, 421, 789
BcgI CGANNNNNNTGC 4 cut(s) 685, 716, 719, 750
BciT130I CCWGG 4 cut(s) 64, 614, 1127, 1176
BclI TGATCA 1 cut(s) 1023
BcnI CCSGG 3 cut(s) 600, 970, 1209
BcoDI GTCTC 3 cut(s) 497, 497, 503
BfoI RGCGCY 1 cut(s) 1185
BglII AGATCT 1 cut(s) 570
BisI GCNGC 9 cut(s) 74, 77, 335, 411, 423, 467, 470, 485, 707
BlsI GCNGC 9 cut(s) 75, 78, 336, 412, 424, 468, 471, 486, 708
Bme1390I CCNGG 7 cut(s) 64, 600, 614, 970, 1127, 1176, 1209
Bme18I GGWCC 2 cut(s) 979, 1205
BmeT110I CYCGRG 2 cut(s) 500, 676
BmgT120I GGNCC 4 cut(s) 979, 1093, 1205, 1211
BmiI GGNNCC 8 cut(s) 237, 693, 981, 1043, 1132, 1195, 1207, 1213
BmrFI CCNGG 7 cut(s) 64, 600, 614, 970, 1127, 1176, 1209
BmsI GCATC 5 cut(s) 250, 690, 724, 844, 892
BpiI GAAGAC 1 cut(s) 715
BpmI CTGGAG 3 cut(s) 1055, 1109, 1164
Bpu10I CCTNAGC 2 cut(s) 80, 762
BpuMI CCSGG 3 cut(s) 600, 970, 1209
Bsa29I ATCGAT 1 cut(s) 165
BsaI GGTCTC 2 cut(s) 497, 503
BsaJI CCNNGG 4 cut(s) 63, 536, 984, 1126
BsaWI WCCGGW 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 441, 513, 1051
Bse118I RCCGGY 3 cut(s) 434, 509, 1133
Bse1I ACTGG 2 cut(s) 1045, 1195
BseAI TCCGGA 1 cut(s) 27
BseBI CCWGG 4 cut(s) 64, 614, 1127, 1176
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 4 cut(s) 63, 536, 984, 1126
BseGI GGATG 5 cut(s) 265, 681, 859, 907, 961
BseLI CCNNNNNNNGG 4 cut(s) 391, 441, 513, 1051
BseMII CTCAG 5 cut(s) 71, 245, 502, 753, 812
BseNI ACTGG 2 cut(s) 1045, 1195
BseRI GAGGAG 2 cut(s) 845, 896
BseXI GCAGC 3 cut(s) 346, 453, 456
BsgI GTGCAG 2 cut(s) 476, 869
Bsh1236I CGCG 5 cut(s) 189, 191, 410, 637, 986
BshFI GGCC 2 cut(s) 1095, 1212
BshNI GGYRCC 1 cut(s) 1130
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 675, 1074
BsiHKCI CYCGRG 2 cut(s) 500, 676
BsiSI CCGG 8 cut(s) 28, 435, 510, 600, 689, 969, 1134, 1209
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 4 cut(s) 391, 441, 513, 1051
BsmAI GTCTC 3 cut(s) 497, 497, 503
BsmFI GGGAC 1 cut(s) 828
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 2 cut(s) 1095, 1212
Bso31I GGTCTC 2 cut(s) 497, 503
BsoBI CYCGRG 2 cut(s) 500, 676
Bsp1286I GDGCHC 3 cut(s) 240, 675, 1074
Bsp13I TCCGGA 1 cut(s) 27
Bsp143I GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
BspANI GGCC 2 cut(s) 1095, 1212
BspCNI CTCAG 5 cut(s) 72, 246, 501, 754, 813
BspDI ATCGAT 1 cut(s) 165
BspEI TCCGGA 1 cut(s) 27
BspFNI CGCG 5 cut(s) 189, 191, 410, 637, 986
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 8 cut(s) 237, 693, 981, 1043, 1132, 1195, 1207, 1213
BspPI GGATC 2 cut(s) 453, 604
BspT107I GGYRCC 1 cut(s) 1130
BspTNI GGTCTC 2 cut(s) 497, 503
BsrBI CCGCTC 1 cut(s) 487
BsrFI RCCGGY 3 cut(s) 434, 509, 1133
BsrI ACTGG 2 cut(s) 1045, 1195
BssAI RCCGGY 3 cut(s) 434, 509, 1133
BssECI CCNNGG 4 cut(s) 63, 536, 984, 1126
BssMI GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
Bst2UI CCWGG 4 cut(s) 64, 614, 1127, 1176
Bst4CI ACNGT 2 cut(s) 526, 949
Bst6I CTCTTC 2 cut(s) 98, 884
BstAPI GCANNNNNTGC 3 cut(s) 340, 463, 911
BstC8I GCNNGC 5 cut(s) 91, 420, 511, 704, 1135
BstDEI CTNAG 5 cut(s) 80, 254, 488, 762, 821
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 5 cut(s) 265, 681, 859, 907, 961
BstFNI CGCG 5 cut(s) 189, 191, 410, 637, 986
BstH2I RGCGCY 1 cut(s) 1185
BstHHI GCGC 4 cut(s) 191, 410, 637, 1184
BstKTI GATC 8 cut(s) 429, 448, 573, 612, 714, 837, 1002, 1026
BstMAI GTCTC 3 cut(s) 497, 497, 503
BstMBI GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
BstMWI GCNNNNNNNGC 6 cut(s) 340, 407, 419, 463, 911, 1139
BstNI CCWGG 4 cut(s) 64, 614, 1127, 1176
BstNSI RCATGY 2 cut(s) 93, 1174
BstSCI CCNGG 7 cut(s) 62, 598, 612, 968, 1125, 1174, 1207
BstUI CGCG 5 cut(s) 189, 191, 410, 637, 986
BstV1I GCAGC 3 cut(s) 346, 453, 456
BstV2I GAAGAC 1 cut(s) 715
BstX2I RGATCY 1 cut(s) 570
BstYI RGATCY 1 cut(s) 570
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 2 cut(s) 1095, 1212
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 5 cut(s) 265, 681, 859, 907, 961
BtsIMutI CAGTG 1 cut(s) 1188
Cac8I GCNNGC 5 cut(s) 91, 420, 511, 704, 1135
CciI TCATGA 1 cut(s) 286
CfoI GCGC 4 cut(s) 191, 410, 637, 1184
Cfr10I RCCGGY 3 cut(s) 434, 509, 1133
Cfr13I GGNCC 4 cut(s) 979, 1093, 1205, 1211
Cfr42I CCGCGG 1 cut(s) 987
ClaI ATCGAT 1 cut(s) 165
Csp6I GTAC 4 cut(s) 154, 865, 931, 1123
CviAII CATG 5 cut(s) 90, 211, 287, 937, 1171
CviQI GTAC 4 cut(s) 154, 865, 931, 1123
DdeI CTNAG 5 cut(s) 80, 254, 488, 762, 821
DpnI GATC 8 cut(s) 428, 447, 572, 611, 713, 836, 1001, 1025
DpnII GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
DraIII CACNNNGTG 1 cut(s) 16
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 98, 884
EarI CTCTTC 2 cut(s) 98, 884
EciI GGCGGA 4 cut(s) 35, 887, 1067, 1186
Ecl136II GAGCTC 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 240, 675
Eco31I GGTCTC 2 cut(s) 497, 503
Eco47I GGWCC 2 cut(s) 979, 1205
Eco53kI GAGCTC 1 cut(s) 673
Eco88I CYCGRG 2 cut(s) 500, 676
EcoICRI GAGCTC 1 cut(s) 673
EcoO109I RGGNCCY 1 cut(s) 1093
EcoRII CCWGG 4 cut(s) 62, 612, 1125, 1174
EcoT38I GRGCYC 2 cut(s) 240, 675
FaeI CATG 5 cut(s) 93, 214, 290, 940, 1174
FaqI GGGAC 1 cut(s) 828
FatI CATG 5 cut(s) 89, 210, 286, 936, 1170
FauI CCCGC 3 cut(s) 299, 506, 991
FbaI TGATCA 1 cut(s) 1023
FblI GTMKAC 1 cut(s) 311
Fnu4HI GCNGC 9 cut(s) 74, 77, 335, 411, 423, 467, 470, 485, 707
FokI GGATG 5 cut(s) 272, 668, 866, 914, 968
FriOI GRGCYC 2 cut(s) 240, 675
Fsp4HI GCNGC 9 cut(s) 74, 77, 335, 411, 423, 467, 470, 485, 707
GlaI GCGC 4 cut(s) 190, 409, 636, 1183
GluI GCNGC 9 cut(s) 74, 77, 335, 411, 423, 467, 470, 485, 707
GsuI CTGGAG 3 cut(s) 1055, 1109, 1164
HaeII RGCGCY 1 cut(s) 1185
HaeIII GGCC 2 cut(s) 1095, 1212
HapII CCGG 8 cut(s) 28, 435, 510, 600, 689, 969, 1134, 1209
HhaI GCGC 4 cut(s) 191, 410, 637, 1184
Hin1II CATG 5 cut(s) 93, 214, 290, 940, 1174
Hin6I GCGC 4 cut(s) 189, 408, 635, 1182
HinP1I GCGC 4 cut(s) 189, 408, 635, 1182
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HinfI GANTC 7 cut(s) 7, 109, 146, 283, 318, 532, 745
HpaII CCGG 8 cut(s) 28, 435, 510, 600, 689, 969, 1134, 1209
HphI GGTGA 4 cut(s) 16, 793, 898, 1033
Hpy166II GTNNAC 2 cut(s) 312, 1015
Hpy188I TCNGA 7 cut(s) 255, 450, 537, 555, 589, 696, 750
Hpy8I GTNNAC 2 cut(s) 312, 1015
Hpy99I CGWCG 3 cut(s) 181, 245, 392
HpyAV CCTTC 9 cut(s) 37, 152, 355, 434, 617, 637, 889, 1000, 1075
HpyCH4III ACNGT 2 cut(s) 526, 949
HpyCH4V TGCA 6 cut(s) 343, 418, 457, 850, 905, 1101
HpyF10VI GCNNNNNNNGC 6 cut(s) 340, 407, 419, 463, 911, 1139
HpyF3I CTNAG 5 cut(s) 80, 254, 488, 762, 821
Hsp92II CATG 5 cut(s) 93, 214, 290, 940, 1174
HspAI GCGC 4 cut(s) 189, 408, 635, 1182
Kpn2I TCCGGA 1 cut(s) 27
KroI GCCGGC 2 cut(s) 509, 1133
KroNI GCCGGC 2 cut(s) 511, 1135
Ksp22I TGATCA 1 cut(s) 1023
KspI CCGCGG 1 cut(s) 987
Kzo9I GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
LmnI GCTCC 4 cut(s) 235, 697, 1084, 1199
Lsp1109I GCAGC 3 cut(s) 346, 453, 456
LweI GCATC 5 cut(s) 250, 690, 724, 844, 892
MaeIII GTNAC 1 cut(s) 478
MalI GATC 8 cut(s) 428, 447, 572, 611, 713, 836, 1001, 1025
MbiI CCGCTC 1 cut(s) 487
MboI GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
MboII GAAGA 9 cut(s) 108, 115, 277, 715, 796, 853, 901, 1009, 1129
MfeI CAATTG 1 cut(s) 1027
MflI RGATCY 1 cut(s) 570
MhlI GDGCHC 3 cut(s) 240, 675, 1074
MluCI AATT 7 cut(s) 141, 203, 549, 922, 962, 974, 1027
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 2 cut(s) 578, 897
MreI CGCCGGCG 1 cut(s) 509
MroI TCCGGA 1 cut(s) 27
MroNI GCCGGC 2 cut(s) 509, 1133
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 986
MspI CCGG 8 cut(s) 28, 435, 510, 600, 689, 969, 1134, 1209
MspR9I CCNGG 7 cut(s) 64, 600, 614, 970, 1127, 1176, 1209
MunI CAATTG 1 cut(s) 1027
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 4 cut(s) 64, 614, 1127, 1176
MvnI CGCG 5 cut(s) 189, 191, 410, 637, 986
MwoI GCNNNNNNNGC 6 cut(s) 340, 407, 419, 463, 911, 1139
NaeI GCCGGC 2 cut(s) 511, 1135
NciI CCSGG 3 cut(s) 600, 970, 1209
NdeII GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
NgoMIV GCCGGC 2 cut(s) 509, 1133
NlaIII CATG 5 cut(s) 93, 214, 290, 940, 1174
NlaIV GGNNCC 8 cut(s) 237, 693, 981, 1043, 1132, 1195, 1207, 1213
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 2 cut(s) 93, 1174
PaeI GCATGC 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 500
PagI TCATGA 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 551
PctI GAATGC 1 cut(s) 916
PdiI GCCGGC 2 cut(s) 511, 1135
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 6 cut(s) 7, 146, 283, 318, 532, 745
PkrI GCNGC 9 cut(s) 75, 78, 336, 412, 424, 468, 471, 486, 708
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 675
Psp6I CCWGG 4 cut(s) 62, 612, 1125, 1174
PspGI CCWGG 4 cut(s) 62, 612, 1125, 1174
PspN4I GGNNCC 8 cut(s) 237, 693, 981, 1043, 1132, 1195, 1207, 1213
PspPI GGNCC 4 cut(s) 979, 1093, 1205, 1211
PsuI RGATCY 1 cut(s) 570
RsaI GTAC 4 cut(s) 155, 866, 932, 1124
RsaNI GTAC 4 cut(s) 154, 865, 931, 1123
SacI GAGCTC 1 cut(s) 675
SacII CCGCGG 1 cut(s) 987
SalI GTCGAC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 9 cut(s) 74, 77, 335, 411, 423, 467, 470, 485, 707
Sau3AI GATC 8 cut(s) 426, 445, 570, 609, 711, 834, 999, 1023
Sau96I GGNCC 4 cut(s) 979, 1093, 1205, 1211
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 7 cut(s) 64, 600, 614, 970, 1127, 1176, 1209
SduI GDGCHC 3 cut(s) 240, 675, 1074
SfaNI GCATC 5 cut(s) 250, 690, 724, 844, 892
Sfr274I CTCGAG 1 cut(s) 500
Sfr303I CCGCGG 1 cut(s) 987
SgrAI CRCCGGYG 1 cut(s) 509
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 2 cut(s) 979, 1205
SlaI CTCGAG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 1 cut(s) 93
Sse9I AATT 7 cut(s) 141, 203, 549, 922, 962, 974, 1027
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 7 cut(s) 62, 598, 612, 968, 1125, 1174, 1207
TaaI ACNGT 2 cut(s) 526, 949
TaqII GACCGA 1 cut(s) 485
TasI AATT 7 cut(s) 141, 203, 549, 922, 962, 974, 1027
TatI WGTACW 1 cut(s) 864
TauI GCSGC 6 cut(s) 76, 79, 413, 425, 487, 709
TfiI GAWTC 6 cut(s) 7, 146, 283, 318, 532, 745
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 1195
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 3 cut(s) 334, 466, 469
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 275, 636
TspRI CASTG 1 cut(s) 1195
VpaK11BI GGWCC 2 cut(s) 979, 1205
XapI RAATTY 1 cut(s) 962
XceI RCATGY 2 cut(s) 93, 1174
XhoI CTCGAG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.