MD02G1036800.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011506
Physical Location & Seq
Forward (+)
2788861 .. 2791828
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1036800.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGGGTCGGAGAGCACCTCTCAACGCGGCGGCGGGGTTGAAAACAACCGCAAGGGCTACGAGACTCAGGTGGTGTTGAATGTCTACGACCTCACCCCCCTCAATAATTACACCGTCTGGTTCGGTCTCGGAATCTTTCACTCCGGTATTGAAGTCCATGGCAAAGAGTACGGTTTTGGAGCCCATGACTTCCCAGTTAGCGGAGTTTTTGAAGTGGAACCAAGGAGCTGCCCAGGTTTTATTTATCGATGTTCCATTCAACTTGGTCGCATAAATATGCCTCCCTCTGAATTTCGGACATTTATTGAGAATGTTGCGGCAGAGTATCATGGGGATACCTATCATCTCATCACCAAGAATTGCAACCATTTCACAGATGAGATAGCATATAGGTTGACAGAAAAACGGATTCCTGGATGGGTGAATCGGCTTGCTCGTCTAGGTGCCTTATGCAGTTGTCTTCTCCCCGAAAGCCTTCAAGTGACCACTGTTAAACAAATACCTGAGTACCATCACGAGTCAGATGGTACTGAAACTCTATCAATCACAATGCCTCGTGAGTCAGGGGAAGTTGATGACGATCAAGAAAAACGCCTGCTGTCCCCACTGGCTTCACTTTCACCGGTGGCGGGAAGTGGAGATGTAACTTTTGTTAAAGAGGCTCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

224

Amino Acids

24.86

Weight (kDa)

5.37

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 23 - 157 3.4e-48 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 27
AciI CCGC 7 cut(s) 27, 30, 33, 49, 201, 317, 629
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 3 cut(s) 170, 509, 529
AfiI CCNNNNNNNGG 2 cut(s) 200, 629
AgeI ACCGGT 1 cut(s) 622
AgsI TTSAA 6 cut(s) 41, 79, 152, 212, 260, 479
AjnI CCWGG 2 cut(s) 232, 412
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 2 cut(s) 56, 131
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 1 cut(s) 290
AsiGI ACCGGT 1 cut(s) 622
Asp700I GAANNNNTTC 1 cut(s) 474
AsuHPI GGTGA 4 cut(s) 85, 343, 433, 612
BaeI ACNNNNGTAYC 2 cut(s) 491, 524
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 184
BauI CACGAG 2 cut(s) 515, 555
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 215
BccI CCATC 3 cut(s) 411, 518, 519
BciT130I CCWGG 2 cut(s) 234, 414
BciVI GTATCC 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 56, 131
BfaI CTAG 1 cut(s) 440
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 4 cut(s) 28, 31, 229, 318
BlsI GCNGC 4 cut(s) 29, 32, 230, 319
Bme1390I CCNGG 2 cut(s) 234, 414
BmiI GGNNCC 3 cut(s) 181, 219, 445
BmrFI CCNGG 2 cut(s) 234, 414
BmrI ACTGGG 1 cut(s) 188
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 452
BplI GAGNNNNNCTC 2 cut(s) 34, 36
Bsa29I ATCGAT 1 cut(s) 247
BsaBI GATNNNNATC 2 cut(s) 339, 579
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 157, 221, 232
BsaWI WCCGGW 2 cut(s) 143, 622
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 629
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 2 cut(s) 194, 612
Bse8I GATNNNNATC 2 cut(s) 339, 579
BseBI CCWGG 2 cut(s) 234, 414
BseCI ATCGAT 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 157, 221, 232
BseGI GGATG 1 cut(s) 422
BseJI GATNNNNATC 2 cut(s) 339, 579
BseLI CCNNNNNNNGG 2 cut(s) 200, 629
BseMII CTCAG 2 cut(s) 80, 495
BseNI ACTGG 2 cut(s) 194, 612
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 443
BshTI ACCGGT 1 cut(s) 622
BshVI ATCGAT 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 2 cut(s) 144, 623
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 2 cut(s) 200, 629
BsmAI GTCTC 2 cut(s) 56, 131
BsmFI GGGAC 1 cut(s) 586
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 17, 184
Bsp143I GATC 1 cut(s) 580
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 7 cut(s) 27, 30, 33, 49, 201, 317, 629
BspCNI CTCAG 2 cut(s) 79, 496
BspDI ATCGAT 1 cut(s) 247
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 181, 219, 445
BspT107I GGYRCC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 622
BsrI ACTGG 2 cut(s) 194, 612
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 3 cut(s) 157, 221, 232
BssMI GATC 1 cut(s) 580
BssSI CACGAG 2 cut(s) 515, 555
BssT1I CCWWGG 2 cut(s) 157, 221
Bst2BI CACGAG 2 cut(s) 515, 555
Bst2UI CCWGG 2 cut(s) 234, 414
Bst4CI ACNGT 3 cut(s) 115, 173, 490
BstC8I GCNNGC 2 cut(s) 432, 596
BstDEI CTNAG 2 cut(s) 66, 504
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 422
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 1 cut(s) 583
BstMAI GTCTC 2 cut(s) 56, 131
BstMBI GATC 1 cut(s) 580
BstNI CCWGG 2 cut(s) 234, 414
BstSCI CCNGG 2 cut(s) 232, 412
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 452
Bsu15I ATCGAT 1 cut(s) 247
BsuI GTATCC 1 cut(s) 328
BsuTUI ATCGAT 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 422
BtsIMutI CAGTG 2 cut(s) 486, 605
Cac8I GCNNGC 2 cut(s) 432, 596
Cfr10I RCCGGY 1 cut(s) 622
ClaI ATCGAT 1 cut(s) 247
Csp6I GTAC 3 cut(s) 169, 508, 528
CspAI ACCGGT 1 cut(s) 622
CviAII CATG 3 cut(s) 158, 185, 329
CviJI RGCY 7 cut(s) 57, 182, 228, 430, 474, 611, 662
CviKI_1 RGCY 7 cut(s) 57, 182, 228, 430, 474, 611, 662
CviQI GTAC 3 cut(s) 169, 508, 528
DdeI CTNAG 2 cut(s) 66, 504
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 157, 221
Eco24I GRGCYC 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 232, 412
EcoT14I CCWWGG 2 cut(s) 157, 221
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 157, 221
FaeI CATG 3 cut(s) 161, 188, 332
FaiI YATR 8 cut(s) 159, 186, 272, 278, 330, 388, 390, 451
FaqI GGGAC 1 cut(s) 586
FatI CATG 3 cut(s) 157, 184, 328
FauI CCCGC 2 cut(s) 26, 622
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 4 cut(s) 28, 31, 229, 318
FokI GGATG 1 cut(s) 429
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 28, 31, 229, 318
FspBI CTAG 1 cut(s) 440
GluI GCNGC 4 cut(s) 28, 31, 229, 318
HapII CCGG 2 cut(s) 144, 623
Hin1II CATG 3 cut(s) 161, 188, 332
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 6 cut(s) 64, 132, 409, 424, 518, 560
HpaII CCGG 2 cut(s) 144, 623
HphI GGTGA 4 cut(s) 85, 343, 433, 612
Hpy166II GTNNAC 2 cut(s) 85, 396
Hpy188I TCNGA 5 cut(s) 10, 131, 289, 297, 523
Hpy188III TCNNGA 3 cut(s) 515, 557, 584
Hpy8I GTNNAC 2 cut(s) 85, 396
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 3 cut(s) 115, 173, 490
HpyCH4V TGCA 2 cut(s) 363, 453
HpyF3I CTNAG 2 cut(s) 66, 504
Hsp92II CATG 3 cut(s) 161, 188, 332
Kzo9I GATC 1 cut(s) 580
LmnI GCTCC 2 cut(s) 179, 225
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 1 cut(s) 440
MaeIII GTNAC 2 cut(s) 481, 643
MalI GATC 1 cut(s) 582
MboI GATC 1 cut(s) 580
MboII GAAGA 1 cut(s) 452
MhlI GDGCHC 2 cut(s) 17, 184
MluCI AATT 3 cut(s) 106, 290, 358
MlyI GAGTC 3 cut(s) 58, 527, 569
MnlI CCTC 7 cut(s) 28, 101, 110, 291, 295, 564, 652
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 2 cut(s) 492, 654
MslI CAYNNNNRTG 1 cut(s) 275
MspI CCGG 2 cut(s) 144, 623
MspR9I CCNGG 2 cut(s) 234, 414
MvaI CCWGG 2 cut(s) 234, 414
MvnI CGCG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 157
NdeII GATC 1 cut(s) 580
NlaIII CATG 3 cut(s) 161, 188, 332
NlaIV GGNNCC 3 cut(s) 181, 219, 445
NmuCI GTSAC 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 3 cut(s) 132, 409, 424
PfoI TCCNGGA 1 cut(s) 412
PinAI ACCGGT 1 cut(s) 622
PkrI GCNGC 4 cut(s) 29, 32, 230, 319
PleI GAGTC 3 cut(s) 58, 526, 568
PpsI GAGTC 3 cut(s) 58, 526, 568
Psp6I CCWGG 2 cut(s) 232, 412
PspGI CCWGG 2 cut(s) 232, 412
PspN4I GGNNCC 3 cut(s) 181, 219, 445
RsaI GTAC 3 cut(s) 170, 509, 529
RsaNI GTAC 3 cut(s) 169, 508, 528
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 492, 654
SatI GCNGC 4 cut(s) 28, 31, 229, 318
Sau3AI GATC 1 cut(s) 580
SchI GAGTC 3 cut(s) 58, 527, 569
ScrFI CCNGG 2 cut(s) 234, 414
SduI GDGCHC 2 cut(s) 17, 184
SetI ASST 9 cut(s) 20, 72, 93, 230, 238, 341, 395, 445, 505
SgrAI CRCCGGYG 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 3 cut(s) 106, 290, 358
SsiI CCGC 7 cut(s) 27, 30, 33, 49, 201, 317, 629
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 2 cut(s) 232, 412
StyI CCWWGG 2 cut(s) 157, 221
TaaI ACNGT 3 cut(s) 115, 173, 490
TaqI TCGA 1 cut(s) 247
TaqII GACCGA 1 cut(s) 113
TasI AATT 3 cut(s) 106, 290, 358
TauI GCSGC 3 cut(s) 30, 33, 320
TfiI GAWTC 3 cut(s) 132, 409, 424
Tru1I TTAA 2 cut(s) 492, 654
Tru9I TTAA 2 cut(s) 492, 654
TscAI CASTG 2 cut(s) 493, 612
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 481
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 2 cut(s) 493, 612
XapI RAATTY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.