Rroxscaffold_2G00152120

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011506
Physical Location & Seq
Reverse (-)
89203077 .. 89205669
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152120.1

Sequence Viewer

Length: 669 bp
ATGGGGGCCGAGGGCACGTCCAGTGGGATTGATCGGAACGGCAAGAGCTACGAGAGTCCGGTGGTCTTGAATGTCTACGACCTCACCCCCATCAACAATTATTCTGTTTGGTTCGGTCTCGGAATATTCCACTCCGGTATTGAAGTTCATGGTAAAGAGTATGGTTTTGGAGCCCATGACTTCCCAGTTAGTGGAGTTTTTGAAGTGGAACCAAAGGCCTGCCCGGGTTTCATTTACCGATGCTCTATTTCACTTGGTCGCATAAACATGCCTCCTTCTGAATTTCGGACATTTATTGAGAATGTTGCTGCAGAGTATCATGGGGATACATATCACCTCATCTCCAAGAATTGCAACCATTTTACCGATGACATAGCATGTCGGTTGACAGAAAAACGGATTCCTGGATGGGTGAACCGGCTTGCTCGGCTAGGTTCCTTGTGCAGCTGTCTTCTTCCAGAAAGCCTTCAAGTGACTACTGTTAAACAGACTCCTGAATACCATCACGAGTCCGAAGAAGATGGTACTGAATCTCTAACAGTCACCACGCCTCGTGAGTCGACAGAAGTTGATGACGATCAAGAAAAGCGTCTGCTGTCACCATTGGCTGCAGCGTCACCGATGTCTGGATGCGGGGATGTGACTTTTGTTAAAGAGACTCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

222

Amino Acids

24.55

Weight (kDa)

5.15

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 20 - 154 7.9e-48 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 191
AccI GTMKAC 2 cut(s) 75, 560
AciI CCGC 1 cut(s) 633
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 1 cut(s) 526
AfiI CCNNNNNNNGG 2 cut(s) 191, 626
AgsI TTSAA 4 cut(s) 70, 143, 203, 470
AjiI CACGTC 1 cut(s) 18
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 2 cut(s) 48, 447
AluI AGCT 2 cut(s) 48, 447
Alw26I GTCTC 2 cut(s) 122, 650
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 2 cut(s) 6, 216
ApeKI GCWGC 4 cut(s) 308, 444, 608, 611
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 465
AspS9I GGNCC 1 cut(s) 6
AsuC2I CCSGG 2 cut(s) 224, 225
AsuHPI GGTGA 6 cut(s) 76, 326, 424, 535, 591, 609
AvaI CYCGRG 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 175
BauI CACGAG 2 cut(s) 506, 552
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 4 cut(s) 295, 456, 595, 623
BccI CCATC 4 cut(s) 98, 402, 510, 515
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 319
BcnI CCSGG 2 cut(s) 224, 225
BcoDI GTCTC 2 cut(s) 122, 650
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 2 cut(s) 309, 609
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 4 cut(s) 309, 445, 609, 612
BlsI GCNGC 4 cut(s) 310, 446, 610, 613
Bme1390I CCNGG 3 cut(s) 224, 225, 405
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 4 cut(s) 7, 172, 210, 436
BmrFI CCNGG 3 cut(s) 224, 225, 405
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 230, 620
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 443
BpuMI CCSGG 2 cut(s) 224, 225
BsaBI GATNNNNATC 2 cut(s) 330, 576
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 9, 223
BsaWI WCCGGW 2 cut(s) 58, 134
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 626
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 2 cut(s) 21, 185
Bse8I GATNNNNATC 2 cut(s) 330, 576
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 9, 223
BseGI GGATG 3 cut(s) 413, 635, 643
BseJI GATNNNNATC 2 cut(s) 330, 576
BseLI CCNNNNNNNGG 2 cut(s) 191, 626
BseNI ACTGG 2 cut(s) 21, 185
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 4 cut(s) 295, 456, 595, 623
BsgI GTGCAG 1 cut(s) 463
BshFI GGCC 2 cut(s) 8, 218
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 4 cut(s) 59, 135, 224, 418
BslI CCNNNNNNNGG 2 cut(s) 191, 626
BsmAI GTCTC 2 cut(s) 122, 650
BsnI GGCC 2 cut(s) 8, 218
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 17, 175
Bsp143I GATC 2 cut(s) 31, 577
BspACI CCGC 1 cut(s) 633
BspANI GGCC 2 cut(s) 8, 218
BspLI GGNNCC 4 cut(s) 7, 172, 210, 436
BspMAI CTGCAG 2 cut(s) 313, 613
BspTNI GGTCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 417
BsrI ACTGG 2 cut(s) 21, 185
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 9, 223
BssMI GATC 2 cut(s) 31, 577
BssSI CACGAG 2 cut(s) 506, 552
Bst2BI CACGAG 2 cut(s) 506, 552
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 481, 541
BstC8I GCNNGC 2 cut(s) 220, 423
BstF5I GGATG 3 cut(s) 413, 635, 643
BstKTI GATC 2 cut(s) 34, 580
BstMAI GTCTC 2 cut(s) 122, 650
BstMBI GATC 2 cut(s) 31, 577
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 2 cut(s) 271, 381
BstSCI CCNGG 3 cut(s) 222, 223, 403
BstSFI CTRYAG 2 cut(s) 309, 609
BstSLI GKGCMC 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 295, 456, 595, 623
BstV2I GAAGAC 1 cut(s) 443
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 2 cut(s) 8, 218
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 3 cut(s) 413, 635, 643
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 220, 423
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 6
Cfr9I CCCGGG 1 cut(s) 223
CseI GACGC 2 cut(s) 578, 603
Csp6I GTAC 1 cut(s) 525
CviAII CATG 5 cut(s) 149, 176, 268, 320, 378
CviJI RGCY 9 cut(s) 8, 48, 173, 218, 421, 430, 447, 465, 608
CviKI_1 RGCY 9 cut(s) 8, 48, 173, 218, 421, 430, 447, 465, 608
CviQI GTAC 1 cut(s) 525
DpnI GATC 2 cut(s) 33, 579
DpnII GATC 2 cut(s) 31, 577
Eco147I AGGCCT 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 175
FaeI CATG 5 cut(s) 152, 179, 271, 323, 381
FaiI YATR 9 cut(s) 150, 162, 177, 263, 269, 321, 331, 374, 379
FatI CATG 5 cut(s) 148, 175, 267, 319, 377
FauI CCCGC 1 cut(s) 626
FblI GTMKAC 2 cut(s) 75, 560
Fnu4HI GCNGC 4 cut(s) 309, 445, 609, 612
FokI GGATG 3 cut(s) 420, 642, 650
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 309, 445, 609, 612
FspBI CTAG 1 cut(s) 431
GluI GCNGC 4 cut(s) 309, 445, 609, 612
HaeIII GGCC 2 cut(s) 8, 218
HapII CCGG 4 cut(s) 59, 135, 224, 418
HgaI GACGC 2 cut(s) 578, 603
Hin1II CATG 5 cut(s) 152, 179, 271, 323, 381
HincII GTYRAC 2 cut(s) 387, 561
HindII GTYRAC 2 cut(s) 387, 561
HinfI GANTC 7 cut(s) 55, 400, 490, 509, 530, 557, 658
HpaII CCGG 4 cut(s) 59, 135, 224, 418
HphI GGTGA 6 cut(s) 76, 326, 424, 535, 591, 609
Hpy166II GTNNAC 4 cut(s) 76, 387, 415, 561
Hpy188I TCNGA 5 cut(s) 36, 122, 280, 288, 514
Hpy188III TCNNGA 7 cut(s) 67, 458, 494, 506, 554, 581, 627
Hpy8I GTNNAC 4 cut(s) 76, 387, 415, 561
HpyAV CCTTC 2 cut(s) 285, 476
HpyCH4III ACNGT 2 cut(s) 481, 541
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 4 cut(s) 311, 354, 444, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 5 cut(s) 152, 179, 271, 323, 381
Kzo9I GATC 2 cut(s) 31, 577
LmnI GCTCC 1 cut(s) 170
Lsp1109I GCAGC 4 cut(s) 295, 456, 595, 623
LweI GCATC 2 cut(s) 230, 620
MaeI CTAG 1 cut(s) 431
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 5 cut(s) 472, 541, 597, 615, 640
MalI GATC 2 cut(s) 33, 579
MboI GATC 2 cut(s) 31, 577
MboII GAAGA 4 cut(s) 443, 446, 527, 530
MhlI GDGCHC 2 cut(s) 17, 175
MluCI AATT 3 cut(s) 97, 281, 349
MlyI GAGTC 5 cut(s) 64, 484, 518, 566, 652
MnlI CCTC 5 cut(s) 4, 92, 282, 347, 561
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 2 cut(s) 483, 651
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 4 cut(s) 59, 135, 224, 418
MspR9I CCNGG 3 cut(s) 224, 225, 405
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 427
NciI CCSGG 2 cut(s) 224, 225
NdeII GATC 2 cut(s) 31, 577
NlaIII CATG 5 cut(s) 152, 179, 271, 323, 381
NlaIV GGNNCC 4 cut(s) 7, 172, 210, 436
NmeAIII GCCGAG 2 cut(s) 34, 406
NmuCI GTSAC 5 cut(s) 472, 541, 597, 615, 640
NspI RCATGY 2 cut(s) 271, 381
PceI AGGCCT 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 2 cut(s) 400, 530
PflMI CCANNNNNTGG 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 4 cut(s) 310, 446, 610, 613
PleI GAGTC 5 cut(s) 63, 484, 517, 565, 652
PpsI GAGTC 5 cut(s) 63, 484, 517, 565, 652
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 4 cut(s) 7, 172, 210, 436
PspPI GGNCC 1 cut(s) 6
PstI CTGCAG 2 cut(s) 313, 613
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
RseI CAYNNNNRTG 1 cut(s) 266
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 2 cut(s) 483, 651
SatI GCNGC 4 cut(s) 309, 445, 609, 612
Sau3AI GATC 2 cut(s) 31, 577
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 5 cut(s) 64, 484, 518, 566, 652
ScrFI CCNGG 3 cut(s) 224, 225, 405
SduI GDGCHC 2 cut(s) 17, 175
SetI ASST 6 cut(s) 20, 50, 84, 339, 436, 449
SfaNI GCATC 2 cut(s) 230, 620
SfcI CTRYAG 2 cut(s) 309, 609
SmaI CCCGGG 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 3 cut(s) 97, 281, 349
SseBI AGGCCT 1 cut(s) 218
SsiI CCGC 1 cut(s) 633
SspI AATATT 1 cut(s) 126
SspMI CTAG 1 cut(s) 431
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 3 cut(s) 222, 223, 403
TaaI ACNGT 2 cut(s) 481, 541
TaiI ACGT 1 cut(s) 20
TaqI TCGA 1 cut(s) 560
TaqII GACCGA 1 cut(s) 104
TasI AATT 3 cut(s) 97, 281, 349
TfiI GAWTC 2 cut(s) 400, 530
Tru1I TTAA 2 cut(s) 483, 651
Tru9I TTAA 2 cut(s) 483, 651
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 5 cut(s) 472, 541, 597, 615, 640
TseI GCWGC 4 cut(s) 308, 444, 608, 611
Tsp45I GTSAC 5 cut(s) 472, 541, 597, 615, 640
TspDTI ATGAA 2 cut(s) 137, 220
TspGWI ACGGA 1 cut(s) 412
TspMI CCCGGG 1 cut(s) 223
TspRI CASTG 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 191
XapI RAATTY 1 cut(s) 281
XceI RCATGY 2 cut(s) 271, 381
XmaI CCCGGG 1 cut(s) 223
XmiI GTMKAC 2 cut(s) 75, 560
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.