MD02G1048900.v1.1

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013585
Physical Location & Seq
Forward (+)
3846149 .. 3849403
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1048900.v1.1.491

Sequence Viewer

Length: 1641 bp
ATGGGGACCGAAGTGGTGGTCACGACGGACCGAGTCCAGAAGTTCTTCGAGGACCTGGAGGCGCAGAGGGCGATTCTCTCCACCTCTACCAAGCTTTTCACAACCCTCTCCGACCACTTTGATTCCCTCCAGAAATCCATTTCCGAGAATTCCCATTCCCTCGAATCCAAAATCCAATCCCTGGAATCCCGTTCCCGCGAAACCTTACAATCCCTCGACCACCGCGAGGCCTCCATCCCGGAGCGCGAGTCCTCCGCCGCCGCTCGAATCGAGGAGCAGAAAGCCGCGGCCTTGGCCGAGGTGGAGAAGAATGTTTCCGGGGATTTGGATTTGCCGGAGACCCTGAAATCGTTTTGCAGGAAAATGGACTCCCAGGGTTTGGTGAAGTTCATCGTTTCGAAGAGGAAGGAATCAATATCGCTCAGGGCAGAGATTGTACAGGCGATTGCGGAGGCGGTGGATCCGCCAAGATTGGTGCTTGATGCATTGGAAGATTTTTTGGATTCTAAAGCTAAGAAATTCGGGGTTACCGATAAGCGGTGGGCATGCGGGCTGTTAGTTCAGGCTCTGTTTCCGGAGGGTAAGCCCAATGCTGCCGCGAAAGGGCCGGAGTTTTCTAGGAGCATGGTGGAGAGAGCGGCGGGGATTGCAGATACGTGGAAGGCGCAGATGGATGGAGATTCAAACGGTGGTGGCGGGACATTGGGGGCTGCGGAGGCGGTGATGTTTGTGCAAATGCTCGTTGGGTTTCGATTGAAAGAGAAGTTCGGTGAGGAGTTATTTAGGAAGTTGGTAATGGAGCACGCAGCGAGGAGGGATATGGCAAAGCTAGCAAGGGCTCTAGAATTCGGAGAGAAAATGGGAGATGTCATTGATGAGTTAGTGAAGAATGGCAAGGAGATAGAGGCTGTATATTTTGCTTCTGAGTCTGATTTGACTGAGAGATTTCCTCCTGTTTCTCTGCTCAAGTCATATCTCAAGAACTCCAGAAAGAATGCTGCGACCATTTTGAAGAATGGCAATAATTGTGTGGCTGCTGCTGAGGAGTCGAGCACATTTGAGTTGAATTCCATCAAAGCAATCATCAAATGTGTAGAAGACCACAAGCTTGAATCAGAGTTTTCTCTTGAGAACTTAAGAAAGCGGGCCACTCATCTGGAGAAGGCCAGGACAGAAAGGAAGAAGAGATCAGCATCTTCCAGCAGGCCTCATAATAATAAGCGAGCCTACGGTGGCAGTCGAGGTGGTGGCAGCGGCAGTGGCGGGGGTGGTGGCCGAGGGGTTGGACAGTCTTCTTCCCGCCCTGCCAAAGCCGCCAAGTTTTCCAATGCGTACCCCTCCACCCGCAGGAACTCAAATCCTCTTCCACAGCATAGCCCCGCAACAAGATACTCTGGTCCTTATAATTACCCGAGCCAAAGTGTGTACGATGGTCCAACAGTAGCATCATATGGATCAACATACGGAGCTCCCCCTGCTCAAAGCCCCGCTGCTCTTCCGTCGCAACACTACTCTGGGGACAGCATGGGTGCTGCCGGTTTCCGAAGTAGTGGTGCTTATGGTGGCCAGACCAACTACGGGCCATATGATTACGCAAGTGCTGCTCCACCAGCATACCAGCCTCCTTCGTATCCGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

547

Amino Acids

59.18

Weight (kDa)

8.91

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 113 - 396 7.1e-94 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013585)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05070 FvH4_1g05070 FvH4_1g05070
malus_domestica MD02G1048900.v1.1 MD15G1187200.v1.1
prunus_persica Prupe.7G229200_v2.0.a1
pyrus_communis pycom02g04100 pycom15g16770
rosa_chinensis RchiOBHm_Chr2g0090711
rosa_laevigata RLG00000016136
rosa_multiflora Rmu_ssc0000367.1_g000001
rosa_roxburghii Rroxscaffold_2G00150890
rosa_rugosa Rorug02G0009900
rosa_samantha Rh2AG055900 Rh2BG054300 Rh2CG056600 Rh2DG055400
rosa_wichuraiana Rw2G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1404
AccB7I CCANNNNNTGG 2 cut(s) 181, 379
AccBSI CCGCTC 2 cut(s) 263, 638
AccII CGCG 5 cut(s) 198, 225, 246, 287, 599
AccIII TCCGGA 1 cut(s) 574
AclWI GGATC 3 cut(s) 455, 468, 1462
AcoI YGGCCR 3 cut(s) 294, 1273, 1564
AcsI RAATTY 4 cut(s) 148, 518, 845, 1066
AfaI GTAC 3 cut(s) 438, 1334, 1427
AfiI CCNNNNNNNGG 7 cut(s) 181, 226, 379, 537, 603, 1347, 1578
AflII CTTAAG 1 cut(s) 1135
AgsI TTSAA 5 cut(s) 684, 757, 1012, 1066, 1112
AjnI CCWGG 4 cut(s) 54, 180, 372, 1166
AluBI AGCT 5 cut(s) 94, 512, 829, 1108, 1469
AluI AGCT 5 cut(s) 94, 512, 829, 1108, 1469
Alw21I GWGCWC 3 cut(s) 804, 1055, 1471
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 3 cut(s) 455, 468, 1462
AlwNI CAGNNNCTG 1 cut(s) 568
Ama87I CYCGRG 1 cut(s) 1411
Aor13HI TCCGGA 1 cut(s) 574
ApoI RAATTY 4 cut(s) 148, 518, 845, 1066
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 44
AspLEI GCGC 3 cut(s) 64, 246, 667
AspS9I GGNCC 8 cut(s) 6, 28, 52, 605, 1146, 1397, 1433, 1580
AsuC2I CCSGG 2 cut(s) 239, 319
AsuHPI GGTGA 3 cut(s) 394, 733, 782
AsuII TTCGAA 1 cut(s) 398
AsuNHI GCTAGC 1 cut(s) 829
AvaI CYCGRG 1 cut(s) 1411
AvaII GGWCC 5 cut(s) 6, 28, 52, 1397, 1433
BaeI ACNNNNGTAYC 2 cut(s) 1381, 1414
BalI TGGCCA 1 cut(s) 1566
BamHI GGATCC 1 cut(s) 460
BanII GRGCYC 2 cut(s) 841, 1471
BbsI GAAGAC 2 cut(s) 1104, 1284
Bbv12I GWGCWC 3 cut(s) 804, 1055, 1471
BbvCI CCTCAGC 1 cut(s) 1041
BccI CCATC 5 cut(s) 242, 664, 668, 1079, 1424
BciT130I CCWGG 4 cut(s) 56, 182, 374, 1168
BciVI GTATCC 1 cut(s) 1641
BcnI CCSGG 2 cut(s) 239, 319
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 3 cut(s) 618, 830, 842
BfrI CTTAAG 1 cut(s) 1135
BfuI GTATCC 1 cut(s) 1641
Bme1390I CCNGG 6 cut(s) 56, 182, 239, 319, 374, 1168
Bme18I GGWCC 5 cut(s) 6, 28, 52, 1397, 1433
BmeT110I CYCGRG 1 cut(s) 1411
BmgT120I GGNCC 8 cut(s) 6, 28, 52, 605, 1146, 1397, 1433, 1580
BmiI GGNNCC 2 cut(s) 7, 462
BmrFI CCNGG 6 cut(s) 56, 182, 239, 319, 374, 1168
BmsI GCATC 3 cut(s) 472, 1202, 1454
BmtI GCTAGC 1 cut(s) 833
BpiI GAAGAC 2 cut(s) 1104, 1284
BplI GAGNNNNNCTC 2 cut(s) 934, 966
BpmI CTGGAG 4 cut(s) 77, 113, 970, 1178
Bpu10I CCTNAGC 2 cut(s) 422, 1041
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 3 cut(s) 950, 962, 1148
BpuMI CCSGG 2 cut(s) 239, 319
BsaAI YACGTR 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 1192
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 8 cut(s) 180, 285, 291, 297, 318, 372, 373, 1276
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 226, 379, 537, 603, 1347, 1578
Bse118I RCCGGY 1 cut(s) 1535
Bse8I GATNNNNATC 1 cut(s) 1192
BseAI TCCGGA 1 cut(s) 574
BseBI CCWGG 4 cut(s) 56, 182, 374, 1168
BseDI CCNNGG 8 cut(s) 180, 285, 291, 297, 318, 372, 373, 1276
BseGI GGATG 2 cut(s) 234, 679
BseJI GATNNNNATC 1 cut(s) 1192
BseLI CCNNNNNNNGG 7 cut(s) 181, 226, 379, 537, 603, 1347, 1578
BseMII CTCAG 4 cut(s) 436, 915, 930, 1032
BseRI GAGGAG 4 cut(s) 287, 788, 826, 1058
Bsh1236I CGCG 5 cut(s) 198, 225, 246, 287, 599
BsiHKAI GWGCWC 3 cut(s) 804, 1055, 1471
BsiHKCI CYCGRG 1 cut(s) 1411
BsiSI CCGG 6 cut(s) 239, 318, 335, 575, 608, 1536
BslFI GGGAC 3 cut(s) 19, 712, 1532
BslI CCNNNNNNNGG 7 cut(s) 181, 226, 379, 537, 603, 1347, 1578
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 3 cut(s) 19, 712, 1532
BsmI GAATGC 1 cut(s) 1000
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 1411
Bsp119I TTCGAA 1 cut(s) 398
Bsp1286I GDGCHC 4 cut(s) 804, 841, 1055, 1471
Bsp13I TCCGGA 1 cut(s) 574
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 3 cut(s) 460, 1187, 1454
BspCNI CTCAG 4 cut(s) 435, 916, 931, 1033
BspEI TCCGGA 1 cut(s) 574
BspFNI CGCG 5 cut(s) 198, 225, 246, 287, 599
BspLI GGNNCC 2 cut(s) 7, 462
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 3 cut(s) 455, 468, 1462
BspQI GCTCTTC 1 cut(s) 1500
BspT104I TTCGAA 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 332
BsrBI CCGCTC 2 cut(s) 263, 638
BsrFI RCCGGY 1 cut(s) 1535
BsrGI TGTACA 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 1535
BssECI CCNNGG 8 cut(s) 180, 285, 291, 297, 318, 372, 373, 1276
BssMI GATC 3 cut(s) 460, 1187, 1454
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 4 cut(s) 56, 182, 374, 1168
Bst4CI ACNGT 4 cut(s) 689, 1232, 1290, 1441
Bst6I CTCTTC 4 cut(s) 395, 1178, 1368, 1500
BstAFI CTTAAG 1 cut(s) 1135
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 1 cut(s) 436
BstBAI YACGTR 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 7 cut(s) 547, 551, 804, 831, 1146, 1205, 1224
BstDEI CTNAG 5 cut(s) 422, 513, 924, 939, 1041
BstDSI CCRYGG 1 cut(s) 285
BstEII GGTNACC 1 cut(s) 526
BstF5I GGATG 2 cut(s) 234, 679
BstFNI CGCG 5 cut(s) 198, 225, 246, 287, 599
BstHHI GCGC 3 cut(s) 64, 246, 667
BstKTI GATC 3 cut(s) 463, 1190, 1457
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 3 cut(s) 460, 1187, 1454
BstNI CCWGG 4 cut(s) 56, 182, 374, 1168
BstNSI RCATGY 1 cut(s) 549
BstPI GGTNACC 1 cut(s) 526
BstSCI CCNGG 6 cut(s) 54, 180, 237, 317, 372, 1166
BstUI CGCG 5 cut(s) 198, 225, 246, 287, 599
BstV2I GAAGAC 2 cut(s) 1104, 1284
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 1156
BstYI RGATCY 1 cut(s) 460
BsuI GTATCC 1 cut(s) 1641
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 234, 679
BtsI GCAGTG 1 cut(s) 1264
BtsIMutI CAGTG 1 cut(s) 1264
Cac8I GCNNGC 7 cut(s) 547, 551, 804, 831, 1146, 1205, 1224
CaiI CAGNNNCTG 1 cut(s) 568
CfoI GCGC 3 cut(s) 64, 246, 667
Cfr10I RCCGGY 1 cut(s) 1535
Cfr13I GGNCC 8 cut(s) 6, 28, 52, 605, 1146, 1397, 1433, 1580
Cfr42I CCGCGG 1 cut(s) 288
CpoI CGGWCCG 1 cut(s) 28
Csp6I GTAC 3 cut(s) 437, 1333, 1426
CspI CGGWCCG 1 cut(s) 28
CviAII CATG 3 cut(s) 546, 625, 1525
CviQI GTAC 3 cut(s) 437, 1333, 1426
DdeI CTNAG 5 cut(s) 422, 513, 924, 939, 1041
DpnI GATC 3 cut(s) 462, 1189, 1456
DpnII GATC 3 cut(s) 460, 1187, 1454
EaeI YGGCCR 3 cut(s) 294, 1273, 1564
Eam1104I CTCTTC 4 cut(s) 395, 1178, 1368, 1500
EarI CTCTTC 4 cut(s) 395, 1178, 1368, 1500
EciI GGCGGA 2 cut(s) 244, 453
Ecl136II GAGCTC 1 cut(s) 1469
Eco130I CCWWGG 1 cut(s) 291
Eco147I AGGCCT 2 cut(s) 230, 1207
Eco24I GRGCYC 2 cut(s) 841, 1471
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 5 cut(s) 6, 28, 52, 1397, 1433
Eco53kI GAGCTC 1 cut(s) 1469
Eco88I CYCGRG 1 cut(s) 1411
Eco91I GGTNACC 1 cut(s) 526
EcoICRI GAGCTC 1 cut(s) 1469
EcoO109I RGGNCCY 1 cut(s) 52
EcoO65I GGTNACC 1 cut(s) 526
EcoRI GAATTC 3 cut(s) 148, 845, 1066
EcoRII CCWGG 4 cut(s) 54, 180, 372, 1166
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 2 cut(s) 841, 1471
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 3 cut(s) 549, 628, 1528
FaqI GGGAC 3 cut(s) 19, 712, 1532
FatI CATG 3 cut(s) 545, 624, 1524
FauNDI CATATG 2 cut(s) 1450, 1585
FokI GGATG 2 cut(s) 221, 686
FriOI GRGCYC 2 cut(s) 841, 1471
FspBI CTAG 3 cut(s) 618, 830, 842
GlaI GCGC 3 cut(s) 63, 245, 666
GsuI CTGGAG 4 cut(s) 77, 113, 970, 1178
HapII CCGG 6 cut(s) 239, 318, 335, 575, 608, 1536
HhaI GCGC 3 cut(s) 64, 246, 667
Hin1II CATG 3 cut(s) 549, 628, 1528
Hin6I GCGC 3 cut(s) 62, 244, 665
HinP1I GCGC 3 cut(s) 62, 244, 665
HindIII AAGCTT 2 cut(s) 92, 1106
HpaII CCGG 6 cut(s) 239, 318, 335, 575, 608, 1536
HphI GGTGA 3 cut(s) 394, 733, 782
Hpy166II GTNNAC 1 cut(s) 1426
Hpy188I TCNGA 7 cut(s) 112, 145, 851, 925, 931, 1117, 1544
Hpy188III TCNNGA 9 cut(s) 22, 37, 130, 575, 842, 979, 987, 1127, 1157
Hpy8I GTNNAC 1 cut(s) 1426
Hpy99I CGWCG 2 cut(s) 28, 1504
HpyAV CCTTC 4 cut(s) 400, 655, 1156, 1635
HpyCH4III ACNGT 4 cut(s) 689, 1232, 1290, 1441
HpyCH4IV ACGT 1 cut(s) 656
HpyCH4V TGCA 4 cut(s) 357, 485, 650, 733
HpyF3I CTNAG 5 cut(s) 422, 513, 924, 939, 1041
HpySE526I ACGT 1 cut(s) 656
Hsp92II CATG 3 cut(s) 549, 628, 1528
HspAI GCGC 3 cut(s) 62, 244, 665
Kpn2I TCCGGA 1 cut(s) 574
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 3 cut(s) 460, 1187, 1454
LguI GCTCTTC 1 cut(s) 1500
LmnI GCTCC 7 cut(s) 241, 274, 621, 799, 1466, 1474, 1609
LweI GCATC 3 cut(s) 472, 1202, 1454
MaeI CTAG 3 cut(s) 618, 830, 842
MaeII ACGT 1 cut(s) 656
MaeIII GTNAC 2 cut(s) 19, 526
MalI GATC 3 cut(s) 462, 1189, 1456
MbiI CCGCTC 2 cut(s) 263, 638
MboI GATC 3 cut(s) 460, 1187, 1454
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 4 cut(s) 804, 841, 1055, 1471
MlsI TGGCCA 1 cut(s) 1566
MluCI AATT 6 cut(s) 148, 518, 845, 1024, 1066, 1405
MluNI TGGCCA 1 cut(s) 1566
MlyI GAGTC 5 cut(s) 42, 257, 362, 935, 1055
MmeI TCCRAC 3 cut(s) 135, 1264, 1460
Mox20I TGGCCA 1 cut(s) 1566
Mph1103I ATGCAT 1 cut(s) 487
MroI TCCGGA 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 44
MscI TGGCCA 1 cut(s) 1566
MseI TTAA 1 cut(s) 1136
Msp20I TGGCCA 1 cut(s) 1566
MspA1I CMGCKG 3 cut(s) 287, 1254, 1490
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 6 cut(s) 239, 318, 335, 575, 608, 1536
MspR9I CCNGG 6 cut(s) 56, 182, 239, 319, 374, 1168
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 4 cut(s) 56, 182, 374, 1168
MvnI CGCG 5 cut(s) 198, 225, 246, 287, 599
NciI CCSGG 2 cut(s) 239, 319
NdeI CATATG 2 cut(s) 1450, 1585
NdeII GATC 3 cut(s) 460, 1187, 1454
NheI GCTAGC 1 cut(s) 829
NlaIII CATG 3 cut(s) 549, 628, 1528
NlaIV GGNNCC 2 cut(s) 7, 462
NmeAIII GCCGAG 2 cut(s) 322, 1301
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 487
NspI RCATGY 1 cut(s) 549
NspV TTCGAA 1 cut(s) 398
PaeI GCATGC 1 cut(s) 549
PasI CCCWGGG 1 cut(s) 373
PceI AGGCCT 2 cut(s) 230, 1207
PciSI GCTCTTC 1 cut(s) 1500
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 528
PctI GAATGC 1 cut(s) 1000
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 9 cut(s) 73, 122, 164, 185, 267, 410, 503, 680, 1112
PflFI GACNNNGTC 1 cut(s) 32
PflMI CCANNNNNTGG 2 cut(s) 181, 379
PfoI TCCNGGA 1 cut(s) 237
PleI GAGTC 5 cut(s) 41, 256, 362, 934, 1054
PpsI GAGTC 5 cut(s) 41, 256, 362, 934, 1054
Ppu21I YACGTR 1 cut(s) 657
PpuMI RGGWCCY 1 cut(s) 52
PsiI TTATAA 1 cut(s) 1404
Psp124BI GAGCTC 1 cut(s) 1471
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 4 cut(s) 54, 180, 372, 1166
PspEI GGTNACC 1 cut(s) 526
PspGI CCWGG 4 cut(s) 54, 180, 372, 1166
PspN4I GGNNCC 2 cut(s) 7, 462
PspPI GGNCC 8 cut(s) 6, 28, 52, 605, 1146, 1397, 1433, 1580
PspPPI RGGWCCY 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 568
PsuI RGATCY 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 3 cut(s) 438, 1334, 1427
RsaNI GTAC 3 cut(s) 437, 1333, 1426
Rsr2I CGGWCCG 1 cut(s) 28
RsrII CGGWCCG 1 cut(s) 28
SacI GAGCTC 1 cut(s) 1471
SacII CCGCGG 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 1500
SaqAI TTAA 1 cut(s) 1136
Sau3AI GATC 3 cut(s) 460, 1187, 1454
Sau96I GGNCC 8 cut(s) 6, 28, 52, 605, 1146, 1397, 1433, 1580
SchI GAGTC 5 cut(s) 42, 257, 362, 935, 1055
ScrFI CCNGG 6 cut(s) 56, 182, 239, 319, 374, 1168
SduI GDGCHC 4 cut(s) 804, 841, 1055, 1471
SfaNI GCATC 3 cut(s) 472, 1202, 1454
Sfr303I CCGCGG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 398
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 5 cut(s) 6, 28, 52, 1397, 1433
SmlI CTYRAG 4 cut(s) 965, 977, 1127, 1135
SmoI CTYRAG 4 cut(s) 965, 977, 1127, 1135
SphI GCATGC 1 cut(s) 549
Sse9I AATT 6 cut(s) 148, 518, 845, 1024, 1066, 1405
SseBI AGGCCT 2 cut(s) 230, 1207
SspMI CTAG 3 cut(s) 618, 830, 842
SstI GAGCTC 1 cut(s) 1471
StuI AGGCCT 2 cut(s) 230, 1207
StyD4I CCNGG 6 cut(s) 54, 180, 237, 317, 372, 1166
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 4 cut(s) 689, 1232, 1290, 1441
TaiI ACGT 1 cut(s) 659
TaqI TCGA 9 cut(s) 48, 162, 216, 265, 270, 398, 751, 1049, 1240
TaqII GACCGA 2 cut(s) 23, 45
TasI AATT 6 cut(s) 148, 518, 845, 1024, 1066, 1405
TatI WGTACW 1 cut(s) 436
TauI GCSGC 8 cut(s) 260, 263, 287, 290, 599, 641, 1257, 1316
TfiI GAWTC 9 cut(s) 73, 122, 164, 185, 267, 410, 503, 680, 1112
Tru1I TTAA 1 cut(s) 1136
Tru9I TTAA 1 cut(s) 1136
TscAI CASTG 1 cut(s) 1264
TseFI GTSAC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 3 cut(s) 41, 1479, 1488
TspRI CASTG 1 cut(s) 1264
Tth111I GACNNNGTC 1 cut(s) 32
Van91I CCANNNNNTGG 2 cut(s) 181, 379
Vha464I CTTAAG 1 cut(s) 1135
VpaK11BI GGWCC 5 cut(s) 6, 28, 52, 1397, 1433
XapI RAATTY 4 cut(s) 148, 518, 845, 1066
XbaI TCTAGA 1 cut(s) 841
XceI RCATGY 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 3 cut(s) 618, 830, 842
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.