Rh2DG055400

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013585
Physical Location & Seq
Forward (+)
4028303 .. 4031555
3253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG055400.1

Sequence Viewer

Length: 1656 bp
ATGGGGACCGAACTGGTGGTCGCGACGGACCGAGTCCAGAAGTTCTTCGACGACCTAGAGGCCCAAAAGGCCATCCTCTCCACCTCCACCAAGCTGTACGCGACCCTCTCCGATCACTTCAACGCCCTCCACAAATCGCTCGCCGAGAAATCCCAATCCCTCGACGCCAAAATCGAATCCCTAGAATCGCGTTCCCGCCAAACTCTAGAGTCCCTCGACCTCCGGGAGATCTCGCTCCCGGAGCGCGAGGCCTCAGCCGCGGCCCGCGTTTCGGAGCAGAAAGGCGCCGCCTTGGCCGAGTTCGAGCGAAACGTTTCGGGGAGTTTGGACATCGCGGAAACGCTGAAGTCGTTGTGTAGAAAAATGGACTCGGAGTGGTTGCTGAAGTTCATAGTTTCGAAGCGGAAGGAGTCGGTGTCGCTGAGGGCGGAGATTGTTCAGGCCATGGCGGAGGCAGTGGACGCGCCGAGGCTGGTGTTGGATGCGTTGGAGGGGTTTCTGGAGACCAAGGCCAAGAAAGTTGGGGTCACTGATAAGCGGTGGGCCTGTGGGCTTTTGGTTCAGGCTTTGTTTCCGGATGTGAGGCCGAATTCGGGCCCGAATGGGCCGGCATTTGCCCGGAGTGTGGTGGAGAGAGCGGCGGGGGTTGCGGACACGTGGAAGGGGCAGATGGATGAGCTGAACAGGAGCGGTGGAGGGACGTTGGGGGCGGCGGAGGCGGTGATGTTTGTGCAGATGGTGATTGGGTTTCAGTTGAGAGAGAGGTACAATGAGGAGTTTTATAAGAAGTTGGTGATGGAACATGCTGCTAGGAGAGATATGGCAAGGCTTGCAGGGGCTTTACAATTCGGAGATATCGGAGATGTTATTGATGAGTTAGTGAAGAATGGCAAGGAGATAGAGGCAATATATTTTGCTTTTGAGTCTGGTTTGACAGAGAGGTTTCCTCCGATTTCCCTTCTTAAGTCCTATTTGAAGAACTTGAAGAAGAATGCTTCAACCATAATGAAGAATGGAAACAATAGTGTGGCTGCATCTGAGGAGTCGAGCACTTTGGAGTTGAATTCCATCAAAGCAATGATCAAATGTGTAGAGGACCACAAGCTTGAATCAGAGTTTTCACTCGAATCCCTAAGGAAGCGAGCCACTGCTCTAGAGAAAGCCAAAGCAGATAGAAAGAAGAATTCAGCAGCAGCTTCCGGTAAGTCTAATAACAAGCGTTCCTATGGTGGCGGTGGCGGTGGCGGTGGCGGCGGCCGTGGAGGGGTTGGATCATCTTCTTTCCGGCCAGCCAAAGCAGCCAAATATTCCAATGCATACCCGTCATCCTTCAACCGGAGGATCCCAAATCCTCCTCCACAGCACAGTCCTGTAACAAGATATCCTGGGCCGTACAATTACCCAAGCCCAACCCCAACTGTGTATGACGGCCCAACATCAGTCTCTTACGGAACCTCATATGGAGTAGCCCGTCCTCAAAGCCCTGTTGCTCTTCCACAACAACATATCTACTCCCTCTCTGGGGACAACAGCATGGCTGCTAGTTCTGTATTCCGAAGTGGTGGTTCATATGGTGGCCAGACCAGCTATGGAGGGCCATACGATTATGCAAATGGGACGCCGCCATCTTACCAACCGCTTCCTTATGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

551

Amino Acids

59.64

Weight (kDa)

9.14

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 111 - 396 2.7e-87 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013585)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05070 FvH4_1g05070 FvH4_1g05070
malus_domestica MD02G1048900.v1.1 MD15G1187200.v1.1
prunus_persica Prupe.7G229200_v2.0.a1
pyrus_communis pycom02g04100 pycom15g16770
rosa_chinensis RchiOBHm_Chr2g0090711
rosa_laevigata RLG00000016136
rosa_multiflora Rmu_ssc0000367.1_g000001
rosa_roxburghii Rroxscaffold_2G00150890
rosa_rugosa Rorug02G0009900
rosa_samantha Rh2AG055900 Rh2BG054300 Rh2CG056600 Rh2DG055400
rosa_wichuraiana Rw2G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 284
AccBSI CCGCTC 2 cut(s) 638, 690
AccII CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
AccIII TCCGGA 1 cut(s) 574
AclI AACGTT 1 cut(s) 312
AclWI GGATC 3 cut(s) 1279, 1336, 1349
AcoI YGGCCR 4 cut(s) 294, 1255, 1286, 1576
AcsI RAATTY 3 cut(s) 589, 1063, 1183
AcuI CTGAAG 2 cut(s) 365, 404
AcvI CACGTG 1 cut(s) 657
AcyI GRCGYC 3 cut(s) 165, 285, 1619
AfaI GTAC 3 cut(s) 98, 767, 1394
AfiI CCNNNNNNNGG 7 cut(s) 271, 593, 625, 1264, 1335, 1521, 1522
AflII CTTAAG 1 cut(s) 962
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 7 cut(s) 121, 976, 985, 999, 1063, 1109, 1333
AjnI CCWGG 1 cut(s) 1384
AluBI AGCT 5 cut(s) 94, 679, 1105, 1196, 1587
AluI AGCT 5 cut(s) 94, 679, 1105, 1196, 1587
Alw21I GWGCWC 1 cut(s) 1052
Alw26I GTCTC 2 cut(s) 497, 1447
AlwI GGATC 3 cut(s) 1279, 1336, 1349
Aor13HI TCCGGA 1 cut(s) 574
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 6 cut(s) 806, 1031, 1190, 1193, 1298, 1538
ApoI RAATTY 3 cut(s) 589, 1063, 1183
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 2 cut(s) 44, 313
AspLEI GCGC 3 cut(s) 246, 287, 466
AsuC2I CCSGG 3 cut(s) 224, 239, 619
AsuHPI GGTGA 3 cut(s) 733, 751, 805
AsuII TTCGAA 1 cut(s) 398
AvaII GGWCC 3 cut(s) 6, 28, 1096
AxyI CCTNAGG 1 cut(s) 1133
BaeGI GKGCMC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 1578
BamHI GGATCC 1 cut(s) 1341
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 599
BbrPI CACGTG 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 1052
BbvCI CCTCAGC 2 cut(s) 253, 422
BbvI GCAGC 6 cut(s) 793, 1018, 1202, 1205, 1310, 1525
BccI CCATC 6 cut(s) 80, 664, 730, 790, 1076, 1633
BceAI ACGGC 3 cut(s) 1242, 1375, 1444
BciT130I CCWGG 1 cut(s) 1386
BclI TGATCA 1 cut(s) 1080
BcnI CCSGG 3 cut(s) 224, 239, 619
BcoDI GTCTC 2 cut(s) 497, 1447
BfaI CTAG 6 cut(s) 56, 182, 206, 810, 1154, 1542
BfoI RGCGCY 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 962
BglI GCCNNNNNGGC 2 cut(s) 68, 293
BglII AGATCT 1 cut(s) 228
Bme1390I CCNGG 4 cut(s) 224, 239, 619, 1386
Bme18I GGWCC 3 cut(s) 6, 28, 1096
BmiI GGNNCC 5 cut(s) 7, 286, 597, 1343, 1453
BmrFI CCNGG 4 cut(s) 224, 239, 619, 1386
BmsI GCATC 2 cut(s) 472, 1043
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 2 cut(s) 253, 422
Bpu14I TTCGAA 1 cut(s) 398
BpuMI CCSGG 3 cut(s) 224, 239, 619
BsaAI YACGTR 1 cut(s) 657
BsaHI GRCGYC 3 cut(s) 165, 285, 1619
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 7 cut(s) 258, 291, 444, 467, 507, 1258, 1385
BsaWI WCCGGW 3 cut(s) 574, 1199, 1335
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 7 cut(s) 271, 593, 625, 1264, 1335, 1521, 1522
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 18
Bse21I CCTNAGG 1 cut(s) 1133
Bse3DI GCAATG 1 cut(s) 1083
BseAI TCCGGA 1 cut(s) 574
BseBI CCWGG 1 cut(s) 1386
BseDI CCNNGG 7 cut(s) 258, 291, 444, 467, 507, 1258, 1385
BseGI GGATG 5 cut(s) 72, 487, 583, 679, 1325
BseLI CCNNNNNNNGG 7 cut(s) 271, 593, 625, 1264, 1335, 1521, 1522
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 3 cut(s) 267, 413, 1029
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 3 cut(s) 788, 1055, 1344
BseSI GKGCMC 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 1255
BseXI GCAGC 6 cut(s) 793, 1018, 1202, 1205, 1310, 1525
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
Bsh1285I CGRYCG 1 cut(s) 1258
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 1258
BsiHKAI GWGCWC 1 cut(s) 1052
BsiSI CCGG 8 cut(s) 223, 239, 575, 608, 619, 1200, 1285, 1336
BslFI GGGAC 5 cut(s) 19, 196, 712, 1538, 1630
BslI CCNNNNNNNGG 7 cut(s) 271, 593, 625, 1264, 1335, 1521, 1522
BsmAI GTCTC 2 cut(s) 497, 1447
BsmFI GGGAC 5 cut(s) 19, 196, 712, 1538, 1630
BsmI GAATGC 1 cut(s) 997
Bso31I GGTCTC 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 398
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 599, 1052
Bsp13I TCCGGA 1 cut(s) 574
Bsp143I GATC 5 cut(s) 112, 228, 1080, 1271, 1341
Bsp19I CCATGG 1 cut(s) 444
Bsp68I TCGCGA 1 cut(s) 23
BspCNI CTCAG 3 cut(s) 266, 414, 1030
BspEI TCCGGA 1 cut(s) 574
BspFNI CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
BspLI GGNNCC 5 cut(s) 7, 286, 597, 1343, 1453
BspPI GGATC 3 cut(s) 1279, 1336, 1349
BspQI GCTCTTC 1 cut(s) 1497
BspT104I TTCGAA 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 284
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 497
BsrBI CCGCTC 2 cut(s) 638, 690
BsrDI GCAATG 1 cut(s) 1083
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 7 cut(s) 258, 291, 444, 467, 507, 1258, 1385
BssMI GATC 5 cut(s) 112, 228, 1080, 1271, 1341
BssNI GRCGYC 3 cut(s) 165, 285, 1619
BssT1I CCWWGG 3 cut(s) 291, 444, 507
Bst2UI CCWGG 1 cut(s) 1386
Bst4CI ACNGT 2 cut(s) 1367, 1420
Bst6I CTCTTC 1 cut(s) 1497
BstACI GRCGYC 3 cut(s) 165, 285, 1619
BstAFI CTTAAG 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 830
BstBAI YACGTR 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 6 cut(s) 141, 265, 609, 831, 1143, 1290
BstDEI CTNAG 4 cut(s) 253, 422, 1038, 1133
BstDSI CCRYGG 3 cut(s) 258, 444, 1258
BstF5I GGATG 5 cut(s) 72, 487, 583, 679, 1325
BstFNI CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 3 cut(s) 246, 287, 466
BstKTI GATC 5 cut(s) 115, 231, 1083, 1274, 1344
BstMAI GTCTC 2 cut(s) 497, 1447
BstMBI GATC 5 cut(s) 112, 228, 1080, 1271, 1341
BstMCI CGRYCG 1 cut(s) 1258
BstNI CCWGG 1 cut(s) 1386
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 222, 237, 617, 1384
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
BstV1I GCAGC 6 cut(s) 793, 1018, 1202, 1205, 1310, 1525
BstX2I RGATCY 2 cut(s) 228, 1341
BstYI RGATCY 2 cut(s) 228, 1341
BstZI CGGCCG 1 cut(s) 1255
Bsu36I CCTNAGG 1 cut(s) 1133
BtgI CCRYGG 3 cut(s) 258, 444, 1258
BtgZI GCGATG 1 cut(s) 316
BtsCI GGATG 5 cut(s) 72, 487, 583, 679, 1325
BtsI GCAGTG 2 cut(s) 462, 1146
BtsIMutI CAGTG 3 cut(s) 462, 528, 1146
BtuMI TCGCGA 1 cut(s) 23
Cac8I GCNNGC 6 cut(s) 141, 265, 609, 831, 1143, 1290
CfoI GCGC 3 cut(s) 246, 287, 466
Cfr10I RCCGGY 1 cut(s) 607
Cfr42I CCGCGG 1 cut(s) 261
CpoI CGGWCCG 1 cut(s) 28
CseI GACGC 3 cut(s) 173, 470, 1627
Csp6I GTAC 3 cut(s) 97, 766, 1393
CspI CGGWCCG 1 cut(s) 28
CviAII CATG 3 cut(s) 445, 803, 1534
CviQI GTAC 3 cut(s) 97, 766, 1393
DdeI CTNAG 4 cut(s) 253, 422, 1038, 1133
DinI GGCGCC 1 cut(s) 286
DpnI GATC 5 cut(s) 114, 230, 1082, 1273, 1343
DpnII GATC 5 cut(s) 112, 228, 1080, 1271, 1341
EaeI YGGCCR 4 cut(s) 294, 1255, 1286, 1576
EagI CGGCCG 1 cut(s) 1255
Eam1104I CTCTTC 1 cut(s) 1497
EarI CTCTTC 1 cut(s) 1497
EciI GGCGGA 3 cut(s) 443, 464, 728
EclXI CGGCCG 1 cut(s) 1255
Eco130I CCWWGG 3 cut(s) 291, 444, 507
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 599
Eco31I GGTCTC 1 cut(s) 497
Eco32I GATATC 2 cut(s) 856, 1382
Eco47I GGWCC 3 cut(s) 6, 28, 1096
Eco52I CGGCCG 1 cut(s) 1255
Eco57I CTGAAG 2 cut(s) 365, 404
Eco72I CACGTG 1 cut(s) 657
Eco81I CCTNAGG 1 cut(s) 1133
EcoRI GAATTC 3 cut(s) 589, 1063, 1183
EcoRII CCWGG 1 cut(s) 1384
EcoRV GATATC 2 cut(s) 856, 1382
EcoT14I CCWWGG 3 cut(s) 291, 444, 507
EcoT22I ATGCAT 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 599
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 3 cut(s) 291, 444, 507
FaeI CATG 3 cut(s) 448, 806, 1537
FaqI GGGAC 5 cut(s) 19, 196, 712, 1538, 1630
FatI CATG 3 cut(s) 444, 802, 1533
FauI CCCGC 3 cut(s) 203, 272, 634
FauNDI CATATG 2 cut(s) 1459, 1570
FbaI TGATCA 1 cut(s) 1080
FokI GGATG 5 cut(s) 59, 494, 590, 686, 1312
FriOI GRGCYC 1 cut(s) 599
FspBI CTAG 6 cut(s) 56, 182, 206, 810, 1154, 1542
GlaI GCGC 3 cut(s) 245, 286, 465
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 288
HapII CCGG 8 cut(s) 223, 239, 575, 608, 619, 1200, 1285, 1336
HgaI GACGC 3 cut(s) 173, 470, 1627
HhaI GCGC 3 cut(s) 246, 287, 466
Hin1I GRCGYC 3 cut(s) 165, 285, 1619
Hin1II CATG 3 cut(s) 448, 806, 1537
Hin6I GCGC 3 cut(s) 244, 285, 464
HinP1I GCGC 3 cut(s) 244, 285, 464
HindIII AAGCTT 1 cut(s) 1103
HpaII CCGG 8 cut(s) 223, 239, 575, 608, 619, 1200, 1285, 1336
HphI GGTGA 3 cut(s) 733, 751, 805
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 9 cut(s) 112, 274, 373, 851, 860, 951, 1039, 1114, 1556
Hpy188III TCNNGA 6 cut(s) 22, 37, 206, 500, 575, 1154
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 3 cut(s) 28, 53, 167
HpyAV CCTTC 4 cut(s) 400, 655, 968, 1339
HpyCH4III ACNGT 2 cut(s) 1367, 1420
HpyCH4IV ACGT 3 cut(s) 312, 656, 701
HpyCH4V TGCA 6 cut(s) 733, 833, 1034, 1316, 1610, 1649
HpyF3I CTNAG 4 cut(s) 253, 422, 1038, 1133
HpySE526I ACGT 3 cut(s) 312, 656, 701
Hsp92I GRCGYC 3 cut(s) 165, 285, 1619
Hsp92II CATG 3 cut(s) 448, 806, 1537
HspAI GCGC 3 cut(s) 244, 285, 464
KasI GGCGCC 1 cut(s) 284
Kpn2I TCCGGA 1 cut(s) 574
KroI GCCGGC 1 cut(s) 607
KroNI GCCGGC 1 cut(s) 609
Ksp22I TGATCA 1 cut(s) 1080
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 5 cut(s) 112, 228, 1080, 1271, 1341
LguI GCTCTTC 1 cut(s) 1497
LmnI GCTCC 4 cut(s) 240, 241, 274, 687
Lsp1109I GCAGC 6 cut(s) 793, 1018, 1202, 1205, 1310, 1525
LweI GCATC 2 cut(s) 472, 1043
MaeI CTAG 6 cut(s) 56, 182, 206, 810, 1154, 1542
MaeII ACGT 3 cut(s) 312, 656, 701
MaeIII GTNAC 2 cut(s) 526, 1372
MalI GATC 5 cut(s) 114, 230, 1082, 1273, 1343
MbiI CCGCTC 2 cut(s) 638, 690
MboI GATC 5 cut(s) 112, 228, 1080, 1271, 1341
MboII GAAGA 9 cut(s) 37, 895, 988, 997, 1000, 1021, 1192, 1269, 1484
MflI RGATCY 2 cut(s) 228, 1341
MhlI GDGCHC 2 cut(s) 599, 1052
MlsI TGGCCA 1 cut(s) 1578
MluCI AATT 5 cut(s) 589, 845, 1063, 1183, 1396
MluNI TGGCCA 1 cut(s) 1578
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 6 cut(s) 42, 218, 362, 419, 932, 1052
MmeI TCCRAC 3 cut(s) 459, 468, 1249
Mox20I TGGCCA 1 cut(s) 1578
Mph1103I ATGCAT 1 cut(s) 1318
MroI TCCGGA 1 cut(s) 574
MroNI GCCGGC 1 cut(s) 607
MroXI GAANNNNTTC 2 cut(s) 44, 313
MscI TGGCCA 1 cut(s) 1578
MseI TTAA 1 cut(s) 963
Msp20I TGGCCA 1 cut(s) 1578
MspA1I CMGCKG 1 cut(s) 260
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 8 cut(s) 223, 239, 575, 608, 619, 1200, 1285, 1336
MspR9I CCNGG 4 cut(s) 224, 239, 619, 1386
Mva1269I GAATGC 1 cut(s) 997
MvaI CCWGG 1 cut(s) 1386
MvnI CGCG 8 cut(s) 23, 101, 190, 246, 260, 267, 335, 464
NaeI GCCGGC 1 cut(s) 609
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 3 cut(s) 224, 239, 619
NcoI CCATGG 1 cut(s) 444
NdeI CATATG 2 cut(s) 1459, 1570
NdeII GATC 5 cut(s) 112, 228, 1080, 1271, 1341
NgoMIV GCCGGC 1 cut(s) 607
NlaIII CATG 3 cut(s) 448, 806, 1537
NlaIV GGNNCC 5 cut(s) 7, 286, 597, 1343, 1453
NmeAIII GCCGAG 3 cut(s) 169, 322, 492
NmuCI GTSAC 1 cut(s) 526
NruI TCGCGA 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 1 cut(s) 806
NspV TTCGAA 1 cut(s) 398
PceI AGGCCT 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 1497
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 309, 347
PctI GAATGC 1 cut(s) 997
PdiI GCCGGC 1 cut(s) 609
PdmI GAANNNNTTC 2 cut(s) 44, 313
PfeI GAWTC 4 cut(s) 176, 185, 1109, 1127
PflFI GACNNNGTC 1 cut(s) 32
PfoI TCCNGGA 2 cut(s) 222, 237
PleI GAGTC 6 cut(s) 41, 217, 362, 418, 931, 1051
PluTI GGCGCC 1 cut(s) 288
PmaCI CACGTG 1 cut(s) 657
PmlI CACGTG 1 cut(s) 657
PpsI GAGTC 6 cut(s) 41, 217, 362, 418, 931, 1051
Ppu21I YACGTR 1 cut(s) 657
PsiI TTATAA 1 cut(s) 783
Psp1406I AACGTT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 1384
PspCI CACGTG 1 cut(s) 657
PspGI CCWGG 1 cut(s) 1384
PspN4I GGNNCC 5 cut(s) 7, 286, 597, 1343, 1453
PspOMI GGGCCC 1 cut(s) 595
PsuI RGATCY 2 cut(s) 228, 1341
PsyI GACNNNGTC 1 cut(s) 32
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 3 cut(s) 98, 767, 1394
RsaNI GTAC 3 cut(s) 97, 766, 1393
Rsr2I CGGWCCG 1 cut(s) 28
RsrII CGGWCCG 1 cut(s) 28
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1497
SaqAI TTAA 1 cut(s) 963
Sau3AI GATC 5 cut(s) 112, 228, 1080, 1271, 1341
SchI GAGTC 6 cut(s) 42, 218, 362, 419, 932, 1052
ScrFI CCNGG 4 cut(s) 224, 239, 619, 1386
SduI GDGCHC 2 cut(s) 599, 1052
SfaNI GCATC 2 cut(s) 472, 1043
SfiI GGCCNNNNNGGCC 1 cut(s) 68
SfoI GGCGCC 1 cut(s) 286
Sfr303I CCGCGG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 398
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 3 cut(s) 6, 28, 1096
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
Sse9I AATT 5 cut(s) 589, 845, 1063, 1183, 1396
SseBI AGGCCT 1 cut(s) 251
SspDI GGCGCC 1 cut(s) 284
SspI AATATT 1 cut(s) 1307
SspMI CTAG 6 cut(s) 56, 182, 206, 810, 1154, 1542
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 4 cut(s) 222, 237, 617, 1384
StyI CCWWGG 3 cut(s) 291, 444, 507
TaaI ACNGT 2 cut(s) 1367, 1420
TaiI ACGT 3 cut(s) 315, 659, 704
TaqI TCGA 8 cut(s) 48, 162, 174, 216, 303, 398, 1046, 1125
TaqII GACCGA 2 cut(s) 23, 45
TasI AATT 5 cut(s) 589, 845, 1063, 1183, 1396
TauI GCSGC 8 cut(s) 260, 263, 290, 641, 713, 1254, 1257, 1624
TfiI GAWTC 4 cut(s) 176, 185, 1109, 1127
Tru1I TTAA 1 cut(s) 963
Tru9I TTAA 1 cut(s) 963
TscAI CASTG 3 cut(s) 462, 535, 1153
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 6 cut(s) 806, 1031, 1190, 1193, 1298, 1538
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 3 cut(s) 379, 1022, 1557
TspGWI ACGGA 2 cut(s) 41, 1464
TspRI CASTG 3 cut(s) 462, 535, 1153
Tth111I GACNNNGTC 1 cut(s) 32
Vha464I CTTAAG 1 cut(s) 962
VpaK11BI GGWCC 3 cut(s) 6, 28, 1096
XapI RAATTY 3 cut(s) 589, 1063, 1183
XbaI TCTAGA 2 cut(s) 205, 1153
XceI RCATGY 1 cut(s) 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 1586
XmnI GAANNNNTTC 2 cut(s) 44, 313
XspI CTAG 6 cut(s) 56, 182, 206, 810, 1154, 1542
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.