MD02G1051100.v1.1

3-hydroxyisobutyryl-CoA hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019760
Physical Location & Seq
Reverse (-)
4213529 .. 4216223
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1051100.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGATGGCATAACAGTGGGCTTTGGTATTGGCCTATCAGGTCACGGTCGCTACCGGATCATTACAGAGAGGACTGTTCTTGCTATGCCAGAGAATGTAATTTGTTTGTTCCCAGATGTTGGGTTTTCACGTATAGAGGCAAAGAATCCAGGAGGAGGATCTGTAGGTATATCCCCACAAACAGATGGAACTTTAACAATGCTGCTTATGAAACATAACAGATGGAGAGGTGGAGAGCTTCAAGTTGATTCCGTGATGCATGTTGCAAGCTCCATACGGGGACACAATGTTTCAAGCCAAATTGCTTTTTTGTCATTGACTTCCTTTCCCGACACAGGTACATTAGCCCCGAGTCCTTCGGATACCTGGATCTACTACAAAGTTTGA

Protein Analysis

129

Amino Acids

13.79

Weight (kDa)

6.9

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 1 - 51 2.2e-17 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019760)

Species Orthologous Gene IDs
malus_domestica MD02G1051100.v1.1 MD02G1054100.v1.1 MD02G1054200.v1.1 MD02G1055400.v1.1 MD02G1055800.v1.1 MD15G1189700.v1.1
pyrus_communis pycom02g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 119
AclWI GGATC 3 cut(s) 65, 166, 377
AfaI GTAC 1 cut(s) 340
AfiI CCNNNNNNNGG 3 cut(s) 119, 155, 335
AgsI TTSAA 2 cut(s) 242, 294
AjnI CCWGG 2 cut(s) 148, 365
AluBI AGCT 2 cut(s) 238, 270
AluI AGCT 2 cut(s) 238, 270
AlwI GGATC 3 cut(s) 65, 166, 377
Ama87I CYCGRG 1 cut(s) 349
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 349
BbvI GCAGC 1 cut(s) 189
BccI CCATC 2 cut(s) 179, 216
BciT130I CCWGG 2 cut(s) 150, 367
BciVI GTATCC 1 cut(s) 355
BfmI CTRYAG 1 cut(s) 162
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
Bme1390I CCNGG 2 cut(s) 150, 367
BmeT110I CYCGRG 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 150, 367
BmsI GCATC 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 131
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 155, 335
BseBI CCWGG 2 cut(s) 150, 367
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 119, 155, 335
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 1 cut(s) 33
BsiEI CGRYCG 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 3 cut(s) 119, 155, 335
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 349
Bsp143I GATC 3 cut(s) 57, 158, 369
BspANI GGCC 1 cut(s) 33
BspPI GGATC 3 cut(s) 65, 166, 377
BssMI GATC 3 cut(s) 57, 158, 369
Bst2UI CCWGG 2 cut(s) 150, 367
Bst4CI ACNGT 3 cut(s) 16, 47, 76
BstBAI YACGTR 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 268
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 3 cut(s) 60, 161, 372
BstMBI GATC 3 cut(s) 57, 158, 369
BstMCI CGRYCG 1 cut(s) 49
BstNI CCWGG 2 cut(s) 150, 367
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 148, 365
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 158, 369
BstYI RGATCY 2 cut(s) 158, 369
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 1 cut(s) 33
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 339
CviAII CATG 1 cut(s) 260
CviJI RGCY 6 cut(s) 21, 33, 238, 270, 297, 347
CviKI_1 RGCY 6 cut(s) 21, 33, 238, 270, 297, 347
CviQI GTAC 1 cut(s) 339
DpnI GATC 3 cut(s) 59, 160, 371
DpnII GATC 3 cut(s) 57, 158, 369
Eco88I CYCGRG 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 148, 365
EcoT22I ATGCAT 1 cut(s) 261
FaeI CATG 1 cut(s) 263
FaiI YATR 8 cut(s) 11, 86, 134, 170, 209, 216, 261, 275
FaqI GGGAC 1 cut(s) 294
FatI CATG 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 203
GluI GCNGC 1 cut(s) 203
HaeIII GGCC 1 cut(s) 33
HapII CCGG 1 cut(s) 55
Hin1II CATG 1 cut(s) 263
HinfI GANTC 3 cut(s) 145, 248, 352
HpaII CCGG 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 366
HpyCH4III ACNGT 3 cut(s) 16, 47, 76
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 2 cut(s) 259, 266
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 1 cut(s) 263
Kzo9I GATC 3 cut(s) 57, 158, 369
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 9 cut(s) 24, 68, 102, 126, 135, 162, 321, 352, 379
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 246
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 1 cut(s) 41
MalI GATC 3 cut(s) 59, 160, 371
MboI GATC 3 cut(s) 57, 158, 369
MflI RGATCY 2 cut(s) 158, 369
MluCI AATT 2 cut(s) 99, 300
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 5 cut(s) 63, 130, 146, 149, 221
Mph1103I ATGCAT 1 cut(s) 261
MseI TTAA 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 2 cut(s) 150, 367
MvaI CCWGG 2 cut(s) 150, 367
NdeII GATC 3 cut(s) 57, 158, 369
NlaIII CATG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 261
NspI RCATGY 1 cut(s) 263
PfeI GAWTC 2 cut(s) 145, 248
PflMI CCANNNNNTGG 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 148
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
Ppu21I YACGTR 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 148, 365
PspGI CCWGG 2 cut(s) 148, 365
PsuI RGATCY 2 cut(s) 158, 369
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 3 cut(s) 57, 158, 369
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 2 cut(s) 150, 367
SetI ASST 8 cut(s) 43, 133, 169, 232, 240, 272, 340, 368
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 2 cut(s) 99, 300
StyD4I CCNGG 2 cut(s) 148, 365
TaaI ACNGT 3 cut(s) 16, 47, 76
TaiI ACGT 1 cut(s) 133
TasI AATT 2 cut(s) 99, 300
TfiI GAWTC 2 cut(s) 145, 248
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 224
TspGWI ACGGA 1 cut(s) 241
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 119
XceI RCATGY 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.