MD02G1054200.v1.1

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019760
Physical Location & Seq
Reverse (-)
4384068 .. 4384964
897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1054200.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCCTCCGCCGAGGCGTTCGTCAAGGGCAGCGTCCACCCAAATGGCGTCGCCGACATCACCCTCGGTCGCCCCCCGAGCTCTCAACGCCATGAACCTAGGTATCTTGAACTTTATGGCATCTTATTGAGTACAAATCTATGTTCTTGCATTTGTTATAAGACGAAATGTTGGATTCAAATTTCGAAGAAGCTAGCTTCTTTTTTTTTTAAGAATTTTGTGTTTCTGTTTAAGTGCTATCTGGATGAATGGGAATCTGACCCAAATGTGAAGTATATGGATGTGGAATACAAGAGCTATCTGGATGAATGGGAATCTGACCCAAATGTGAAGTGTGTTCTTGTTGATAGCCGCTCCGCTCGTGCCTTTTGTGGCGGTAACGCCCAGAATTCGTATCTGATTAGTTTCTTATCTTTCTTGGCAATTTTACACTTCCATTTTAGCATTCAGTTAAATAAGTTTTTCTAG

Protein Analysis

156

Amino Acids

17.89

Weight (kDa)

7.57

Isoelectric Point (pI)

23.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019760)

Species Orthologous Gene IDs
malus_domestica MD02G1051100.v1.1 MD02G1054100.v1.1 MD02G1054200.v1.1 MD02G1055400.v1.1 MD02G1055800.v1.1 MD15G1189700.v1.1
pyrus_communis pycom02g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 159
AccBSI CCGCTC 2 cut(s) 354, 359
AciI CCGC 4 cut(s) 9, 352, 357, 375
AcsI RAATTY 3 cut(s) 180, 214, 388
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 2 cut(s) 110, 179
AluBI AGCT 4 cut(s) 81, 193, 197, 297
AluI AGCT 4 cut(s) 81, 193, 197, 297
Alw21I GWGCWC 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 3 cut(s) 180, 214, 388
AspA2I CCTAGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 52
AsuII TTCGAA 1 cut(s) 185
AsuNHI GCTAGC 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 76
AvrII CCTAGG 1 cut(s) 98
BaeI ACNNNNGTAYC 2 cut(s) 86, 119
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 1 cut(s) 42
BfaI CTAG 3 cut(s) 99, 194, 466
BisI GCNGC 2 cut(s) 31, 352
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 2 cut(s) 32, 353
BmeT110I CYCGRG 1 cut(s) 76
BmsI GCATC 1 cut(s) 129
BmtI GCTAGC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 12, 64, 98
BseDI CCNNGG 3 cut(s) 12, 64, 98
BseGI GGATG 3 cut(s) 250, 286, 310
BseXI GCAGC 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 76
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 83
BspACI CCGC 4 cut(s) 9, 352, 357, 375
BspANI GGCC 1 cut(s) 5
BspOI GCTAGC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 185
BsrBI CCGCTC 2 cut(s) 354, 359
BssECI CCNNGG 3 cut(s) 12, 64, 98
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 48
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 195
BstF5I GGATG 3 cut(s) 250, 286, 310
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 78, 87
BstV1I GCAGC 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 44
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 250, 286, 310
Cac8I GCNNGC 1 cut(s) 195
CseI GACGC 2 cut(s) 22, 37
Csp6I GTAC 1 cut(s) 132
CviAII CATG 1 cut(s) 92
CviJI RGCY 6 cut(s) 5, 81, 193, 197, 297, 351
CviKI_1 RGCY 6 cut(s) 5, 81, 193, 197, 297, 351
CviQI GTAC 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 1 cut(s) 95
FaiI YATR 6 cut(s) 93, 117, 142, 159, 276, 278
FatI CATG 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 31, 352
FokI GGATG 3 cut(s) 257, 293, 317
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 31, 352
FspBI CTAG 3 cut(s) 99, 194, 466
GluI GCNGC 2 cut(s) 31, 352
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 22, 37
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 95
HinfI GANTC 3 cut(s) 175, 254, 314
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 259, 319, 399
Hpy188III TCNNGA 3 cut(s) 107, 242, 302
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 53
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 87
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 95
LmnI GCTCC 1 cut(s) 359
LpnPI CCDG 3 cut(s) 227, 287, 398
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 1 cut(s) 129
MaeI CTAG 3 cut(s) 99, 194, 466
MaeIII GTNAC 1 cut(s) 377
MbiI CCGCTC 2 cut(s) 354, 359
MboII GAAGA 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 180, 214, 388, 423
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 3 cut(s) 7, 16, 74
MseI TTAA 3 cut(s) 210, 231, 452
MslI CAYNNNNRTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 78, 87
NheI GCTAGC 1 cut(s) 193
NlaIII CATG 1 cut(s) 95
NmeAIII GCCGAG 1 cut(s) 37
NspV TTCGAA 1 cut(s) 185
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 3 cut(s) 175, 254, 314
PkrI GCNGC 2 cut(s) 32, 353
PsiI TTATAA 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 83
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 42
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 3 cut(s) 210, 231, 452
SatI GCNGC 2 cut(s) 31, 352
SduI GDGCHC 1 cut(s) 83
SetI ASST 6 cut(s) 83, 100, 104, 195, 199, 299
SfaNI GCATC 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 42
Sse9I AATT 4 cut(s) 180, 214, 388, 423
SsiI CCGC 4 cut(s) 9, 352, 357, 375
SspMI CTAG 3 cut(s) 99, 194, 466
SstI GAGCTC 1 cut(s) 83
StyI CCWWGG 1 cut(s) 98
TaqI TCGA 1 cut(s) 185
TaqII GACCGA 1 cut(s) 56
TasI AATT 4 cut(s) 180, 214, 388, 423
TatI WGTACW 1 cut(s) 131
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 3 cut(s) 175, 254, 314
Tru1I TTAA 3 cut(s) 210, 231, 452
Tru9I TTAA 3 cut(s) 210, 231, 452
TseI GCWGC 1 cut(s) 30
TspDTI ATGAA 3 cut(s) 108, 261, 321
XapI RAATTY 3 cut(s) 180, 214, 388
XmaJI CCTAGG 1 cut(s) 98
XspI CTAG 3 cut(s) 99, 194, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.