MD02G1055400.v1.1

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019760
Physical Location & Seq
Reverse (-)
4478042 .. 4480089
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1055400.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGCCTCCACCGACGTGTTCGTCAAGGGCAGCGTCCACCCAAATGGCGTCGCCGACATCACCCTCGGTCGCCCCCCGAGCTCTCAACGCCATGAACCTAGGTATGTAGATAAACACACACACACGCATATGTATATATATATGGATGTGAAATACAAGAGCTATCTGGATGAATGGGAATCTGACCCAAATGTGAAGTGTGTTCTTGTTGATAGCCGCTCCGCTCGTGCCTTTTGTGGTGCCCACATAAGCTTCATGGATGGCATAACAGTGGGCTTTGGTATTGGCCTATCAGGTCACGGTCGCTACCGGATCATTACAGAGAGGACTGTTCTTGCTATGCCAGAGAACGGAATTGGTTTGTTCCCAAATGGGTTTTCACATATAGAGGCAAAGAGTCCGGGAGGAGGATCTGCAGTTCCCAGGCTCCCCATCAGGACATTGAAACAACTGTGGTGA

Protein Analysis

153

Amino Acids

16.81

Weight (kDa)

8.95

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 82 - 135 2.4e-17 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019760)

Species Orthologous Gene IDs
malus_domestica MD02G1051100.v1.1 MD02G1054100.v1.1 MD02G1054200.v1.1 MD02G1055400.v1.1 MD02G1055800.v1.1 MD15G1189700.v1.1
pyrus_communis pycom02g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 239
AccBSI CCGCTC 2 cut(s) 219, 224
AciI CCGC 2 cut(s) 217, 222
AclWI GGATC 2 cut(s) 320, 418
AcyI GRCGYC 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 350, 407
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 1 cut(s) 445
AjiI CACGTC 1 cut(s) 16
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AleI CACNNNNGTG 1 cut(s) 14
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 3 cut(s) 81, 162, 252
AluI AGCT 3 cut(s) 81, 162, 252
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 2 cut(s) 320, 418
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 2 cut(s) 3, 286
ApeKI GCWGC 1 cut(s) 30
AspA2I CCTAGG 1 cut(s) 98
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 76
AvrII CCTAGG 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 1 cut(s) 42
BccI CCATC 2 cut(s) 254, 440
BciT130I CCWGG 1 cut(s) 424
BcnI CCSGG 1 cut(s) 402
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 2 cut(s) 31, 217
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 2 cut(s) 32, 218
Bme1390I CCNGG 2 cut(s) 402, 424
BmeT110I CYCGRG 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 241, 428
BmrFI CCNGG 2 cut(s) 402, 424
BpuMI CCSGG 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 64, 98, 422
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 407
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 3 cut(s) 64, 98, 422
BseGI GGATG 3 cut(s) 151, 175, 265
BseLI CCNNNNNNNGG 2 cut(s) 350, 407
BseRI GAGGAG 1 cut(s) 420
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 1 cut(s) 42
Bsh1285I CGRYCG 2 cut(s) 70, 304
BshFI GGCC 2 cut(s) 5, 288
BshNI GGYRCC 1 cut(s) 239
BsiEI CGRYCG 2 cut(s) 70, 304
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 2 cut(s) 310, 401
BslI CCNNNNNNNGG 2 cut(s) 350, 407
BsnI GGCC 2 cut(s) 5, 288
BsoBI CYCGRG 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 83, 244
Bsp143I GATC 2 cut(s) 312, 410
BspACI CCGC 2 cut(s) 217, 222
BspANI GGCC 2 cut(s) 5, 288
BspLI GGNNCC 2 cut(s) 241, 428
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 2 cut(s) 320, 418
BspT107I GGYRCC 1 cut(s) 239
BsrBI CCGCTC 2 cut(s) 219, 224
BssECI CCNNGG 3 cut(s) 64, 98, 422
BssMI GATC 2 cut(s) 312, 410
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 4 cut(s) 271, 302, 331, 453
BstACI GRCGYC 1 cut(s) 48
BstF5I GGATG 3 cut(s) 151, 175, 265
BstKTI GATC 2 cut(s) 315, 413
BstMBI GATC 2 cut(s) 312, 410
BstMCI CGRYCG 2 cut(s) 70, 304
BstMWI GCNNNNNNNGC 2 cut(s) 78, 87
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 400, 422
BstSFI CTRYAG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 410
BsuRI GGCC 2 cut(s) 5, 288
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 3 cut(s) 151, 175, 265
BtsIMutI CAGTG 1 cut(s) 276
CseI GACGC 2 cut(s) 22, 37
CviAII CATG 2 cut(s) 92, 256
CviJI RGCY 8 cut(s) 5, 81, 162, 216, 252, 276, 288, 427
CviKI_1 RGCY 8 cut(s) 5, 81, 162, 216, 252, 276, 288, 427
DpnI GATC 2 cut(s) 314, 412
DpnII GATC 2 cut(s) 312, 410
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 2 cut(s) 95, 259
FatI CATG 2 cut(s) 91, 255
FauNDI CATATG 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 31, 217
FokI GGATG 3 cut(s) 158, 182, 272
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 31, 217
FspBI CTAG 1 cut(s) 99
GluI GCNGC 2 cut(s) 31, 217
HaeIII GGCC 2 cut(s) 5, 288
HapII CCGG 2 cut(s) 310, 401
HgaI GACGC 2 cut(s) 22, 37
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 95, 259
HindIII AAGCTT 1 cut(s) 250
HinfI GANTC 2 cut(s) 179, 397
HpaII CCGG 2 cut(s) 310, 401
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 2 cut(s) 167, 436
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 2 cut(s) 17, 53
HpyCH4III ACNGT 4 cut(s) 271, 302, 331, 453
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 87
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 95, 259
Kzo9I GATC 2 cut(s) 312, 410
LmnI GCTCC 2 cut(s) 224, 432
LpnPI CCDG 8 cut(s) 152, 279, 323, 357, 409, 414, 421, 436
Lsp1109I GCAGC 1 cut(s) 42
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 296
MalI GATC 2 cut(s) 314, 412
MbiI CCGCTC 2 cut(s) 219, 224
MboI GATC 2 cut(s) 312, 410
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 2 cut(s) 83, 244
MluCI AATT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 6 cut(s) 16, 74, 318, 382, 398, 401
MslI CAYNNNNRTG 4 cut(s) 14, 42, 128, 269
MspI CCGG 2 cut(s) 310, 401
MspR9I CCNGG 2 cut(s) 402, 424
MvaI CCWGG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 78, 87
NciI CCSGG 1 cut(s) 402
NdeI CATATG 1 cut(s) 129
NdeII GATC 2 cut(s) 312, 410
NlaIII CATG 2 cut(s) 95, 259
NlaIV GGNNCC 2 cut(s) 241, 428
NmuCI GTSAC 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 14
PfeI GAWTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 400
PkrI GCNGC 2 cut(s) 32, 218
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 241, 428
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 410
RseI CAYNNNNRTG 4 cut(s) 14, 42, 128, 269
SacI GAGCTC 1 cut(s) 83
SatI GCNGC 2 cut(s) 31, 217
Sau3AI GATC 2 cut(s) 312, 410
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 402, 424
SduI GDGCHC 2 cut(s) 83, 244
SetI ASST 7 cut(s) 18, 83, 100, 104, 164, 254, 298
SfcI CTRYAG 1 cut(s) 414
SmiMI CAYNNNNRTG 4 cut(s) 14, 42, 128, 269
Sse9I AATT 1 cut(s) 354
SsiI CCGC 2 cut(s) 217, 222
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 400, 422
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 4 cut(s) 271, 302, 331, 453
TaiI ACGT 1 cut(s) 18
TaqII GACCGA 1 cut(s) 56
TasI AATT 1 cut(s) 354
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 179
TscAI CASTG 1 cut(s) 276
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 108, 186, 244
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 276
XmaJI CCTAGG 1 cut(s) 98
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.