MD02G1055800.v1.1

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019760
Physical Location & Seq
Reverse (-)
4511734 .. 4514514
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1055800.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCCTCCGCCGACGCGTTCGTCAAGGGCAGCGTCCACCCAATTGGCGTCGCCGTCATCACCCTCGGTCGCCCCCCGAGCTCTCAACGCCAAGAACCTAGGTATCTTGAACTTTATGATACGGATGTGAAATACAAGAGCTATCTGGATGAATGGGAATCTGACCCAAATGTGAAGTGTGACATTAAAGTGGTTATTGCCGAAATTCAAAAGGACAAAAACACACCTCTTGTGTTTAAGGTATTCACTGCTGAGTATTCTCTAATATGCAAAATCTCTGAGTACAAGAAGCCCTATATAAGCTTCATGGATGGCATAACAATGGGCTTTGGTGTTGGCCTATCAGGTCACTGTCGCTACCGGATCATTAGAGAGAGGACTGTTCTTGCTAATGCCAGAGAATGTAATTTGTTTGTTCCGAGATGGTGGGTTTTCGCGTATAGCAGCAAAGAGTCCAGGAGGAGGATCTGTAGGATTTCAACACCATCGGATGCACTGTATGTGGGTCTTGGGACCCATTTTGTCCCATCTCAAAACTTGGCTATTTGTGCCAAAGAATCAAGGTGGAGAGGTGGAGAGCTTCAAGTTGATTCGGTGATGCATGTTGCAAGCATCATAAGGAGGACACAATGTTTCAATCCAGACACGGGTACATTAGCCCCGAGTCCTTCGGATACCTGGGTCTACTACAAAGTTTGA

Protein Analysis

233

Amino Acids

26.38

Weight (kDa)

9.01

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 61 - 130 5.6e-19 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019760)

Species Orthologous Gene IDs
malus_domestica MD02G1051100.v1.1 MD02G1054100.v1.1 MD02G1054200.v1.1 MD02G1055400.v1.1 MD02G1055800.v1.1 MD15G1189700.v1.1
pyrus_communis pycom02g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
AccI GTMKAC 1 cut(s) 684
AccII CGCG 2 cut(s) 17, 437
AciI CCGC 1 cut(s) 9
AclWI GGATC 2 cut(s) 371, 473
AcsI RAATTY 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 284, 652
AfiI CCNNNNNNNGG 2 cut(s) 462, 647
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 5 cut(s) 110, 209, 480, 584, 637
AjnI CCWGG 2 cut(s) 455, 677
AluBI AGCT 4 cut(s) 81, 141, 303, 580
AluI AGCT 4 cut(s) 81, 141, 303, 580
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 2 cut(s) 371, 473
Ama87I CYCGRG 2 cut(s) 76, 661
AoxI GGCC 2 cut(s) 3, 337
ApeKI GCWGC 2 cut(s) 30, 444
ApoI RAATTY 1 cut(s) 204
AspA2I CCTAGG 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 513
AsuHPI GGTGA 2 cut(s) 52, 607
AvaI CYCGRG 2 cut(s) 76, 661
AvaII GGWCC 1 cut(s) 513
AvrII CCTAGG 1 cut(s) 98
BaeI ACNNNNGTAYC 6 cut(s) 86, 111, 119, 144, 666, 699
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 42, 456
BccI CCATC 4 cut(s) 305, 417, 493, 535
BceAI ACGGC 1 cut(s) 38
BciT130I CCWGG 2 cut(s) 457, 679
BciVI GTATCC 1 cut(s) 667
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 469
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 2 cut(s) 31, 445
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 2 cut(s) 32, 446
Bme1390I CCNGG 2 cut(s) 457, 679
Bme18I GGWCC 1 cut(s) 513
BmeT110I CYCGRG 2 cut(s) 76, 661
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 2 cut(s) 514, 515
BmrFI CCNGG 2 cut(s) 457, 679
BmsI GCATC 3 cut(s) 481, 588, 621
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 64, 98, 678
BsaWI WCCGGW 1 cut(s) 360
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 647
BseBI CCWGG 2 cut(s) 457, 679
BseDI CCNNGG 3 cut(s) 64, 98, 678
BseGI GGATG 4 cut(s) 130, 154, 316, 496
BseLI CCNNNNNNNGG 2 cut(s) 462, 647
BseMII CTCAG 2 cut(s) 243, 270
BseRI GAGGAG 1 cut(s) 475
BseXI GCAGC 2 cut(s) 42, 456
Bsh1236I CGCG 2 cut(s) 17, 437
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 5, 339
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 2 cut(s) 76, 661
BsiSI CCGG 1 cut(s) 361
BslFI GGGAC 2 cut(s) 509, 526
BslI CCNNNNNNNGG 2 cut(s) 462, 647
BsmFI GGGAC 2 cut(s) 509, 526
BsnI GGCC 2 cut(s) 5, 339
BsoBI CYCGRG 2 cut(s) 76, 661
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 2 cut(s) 363, 465
BspACI CCGC 1 cut(s) 9
BspANI GGCC 2 cut(s) 5, 339
BspCNI CTCAG 2 cut(s) 244, 271
BspFNI CGCG 2 cut(s) 17, 437
BspLI GGNNCC 2 cut(s) 514, 515
BspPI GGATC 2 cut(s) 371, 473
BssECI CCNNGG 3 cut(s) 64, 98, 678
BssMI GATC 2 cut(s) 363, 465
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 457, 679
Bst4CI ACNGT 3 cut(s) 353, 382, 498
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 2 cut(s) 252, 279
BstF5I GGATG 4 cut(s) 130, 154, 316, 496
BstFNI CGCG 2 cut(s) 17, 437
BstKTI GATC 2 cut(s) 366, 468
BstMBI GATC 2 cut(s) 363, 465
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 3 cut(s) 78, 87, 548
BstNI CCWGG 2 cut(s) 457, 679
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 2 cut(s) 455, 677
BstSFI CTRYAG 1 cut(s) 469
BstUI CGCG 2 cut(s) 17, 437
BstV1I GCAGC 2 cut(s) 42, 456
BstX2I RGATCY 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 465
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 2 cut(s) 5, 339
BtsCI GGATG 4 cut(s) 130, 154, 316, 496
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 3 cut(s) 246, 349, 494
Cac8I GCNNGC 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 513
CseI GACGC 3 cut(s) 22, 23, 37
Csp6I GTAC 2 cut(s) 283, 651
CviAII CATG 2 cut(s) 307, 602
CviQI GTAC 2 cut(s) 283, 651
DdeI CTNAG 2 cut(s) 252, 279
DpnI GATC 2 cut(s) 365, 467
DpnII GATC 2 cut(s) 363, 465
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 513
Eco53kI GAGCTC 1 cut(s) 81
Eco88I CYCGRG 2 cut(s) 76, 661
EcoICRI GAGCTC 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 455, 677
EcoT14I CCWWGG 1 cut(s) 98
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 2 cut(s) 310, 605
FaqI GGGAC 2 cut(s) 509, 526
FatI CATG 2 cut(s) 306, 601
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 2 cut(s) 31, 445
FokI GGATG 4 cut(s) 137, 161, 323, 503
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 31, 445
FspBI CTAG 1 cut(s) 99
GluI GCNGC 2 cut(s) 31, 445
HaeIII GGCC 2 cut(s) 5, 339
HapII CCGG 1 cut(s) 361
HgaI GACGC 3 cut(s) 22, 23, 37
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 310, 605
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 5 cut(s) 158, 452, 557, 590, 664
HpaII CCGG 1 cut(s) 361
HphI GGTGA 2 cut(s) 52, 607
Hpy166II GTNNAC 2 cut(s) 37, 685
Hpy188I TCNGA 5 cut(s) 163, 280, 420, 490, 673
Hpy188III TCNNGA 3 cut(s) 107, 146, 641
Hpy8I GTNNAC 2 cut(s) 37, 685
Hpy99I CGWCG 2 cut(s) 17, 53
HpyAV CCTTC 1 cut(s) 678
HpyCH4III ACNGT 3 cut(s) 353, 382, 498
HpyCH4V TGCA 4 cut(s) 270, 494, 601, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 87, 548
HpyF3I CTNAG 2 cut(s) 252, 279
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 310, 605
KflI GGGWCCC 1 cut(s) 513
Kzo9I GATC 2 cut(s) 363, 465
LpnPI CCDG 9 cut(s) 131, 330, 374, 409, 442, 469, 654, 664, 691
Lsp1109I GCAGC 2 cut(s) 42, 456
LweI GCATC 3 cut(s) 481, 588, 621
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 179, 347
MalI GATC 2 cut(s) 365, 467
MboI GATC 2 cut(s) 363, 465
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 3 cut(s) 42, 204, 406
MluI ACGCGT 1 cut(s) 15
MlyI GAGTC 2 cut(s) 461, 673
MnlI CCTC 8 cut(s) 16, 74, 237, 369, 453, 456, 563, 615
Mph1103I ATGCAT 1 cut(s) 603
MseI TTAA 2 cut(s) 186, 237
MslI CAYNNNNRTG 2 cut(s) 188, 320
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 2 cut(s) 457, 679
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 2 cut(s) 457, 679
MvnI CGCG 2 cut(s) 17, 437
MwoI GCNNNNNNNGC 3 cut(s) 78, 87, 548
NdeII GATC 2 cut(s) 363, 465
NlaIII CATG 2 cut(s) 310, 605
NlaIV GGNNCC 2 cut(s) 514, 515
NmuCI GTSAC 2 cut(s) 179, 347
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 1 cut(s) 605
PfeI GAWTC 3 cut(s) 158, 557, 590
PfoI TCCNGGA 1 cut(s) 455
PkrI GCNGC 2 cut(s) 32, 446
PleI GAGTC 2 cut(s) 460, 672
PpsI GAGTC 2 cut(s) 460, 672
PpuMI RGGWCCY 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 513
Psp6I CCWGG 2 cut(s) 455, 677
PspGI CCWGG 2 cut(s) 455, 677
PspN4I GGNNCC 2 cut(s) 514, 515
PspPI GGNCC 1 cut(s) 513
PspPPI RGGWCCY 1 cut(s) 513
PsuI RGATCY 1 cut(s) 465
RsaI GTAC 2 cut(s) 284, 652
RsaNI GTAC 2 cut(s) 283, 651
RseI CAYNNNNRTG 2 cut(s) 188, 320
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 186, 237
SatI GCNGC 2 cut(s) 31, 445
Sau3AI GATC 2 cut(s) 363, 465
Sau96I GGNCC 1 cut(s) 513
SchI GAGTC 2 cut(s) 461, 673
ScrFI CCNGG 2 cut(s) 457, 679
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 3 cut(s) 481, 588, 621
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 2 cut(s) 188, 320
Sse9I AATT 3 cut(s) 42, 204, 406
SsiI CCGC 1 cut(s) 9
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 455, 677
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 353, 382, 498
TaqII GACCGA 1 cut(s) 56
TasI AATT 3 cut(s) 42, 204, 406
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 3 cut(s) 158, 557, 590
Tru1I TTAA 2 cut(s) 186, 237
Tru9I TTAA 2 cut(s) 186, 237
TscAI CASTG 3 cut(s) 253, 356, 501
TseFI GTSAC 2 cut(s) 179, 347
TseI GCWGC 2 cut(s) 30, 444
Tsp45I GTSAC 2 cut(s) 179, 347
TspDTI ATGAA 2 cut(s) 165, 295
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 3 cut(s) 253, 356, 501
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 1 cut(s) 204
XceI RCATGY 1 cut(s) 605
XmaJI CCTAGG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.