MD02G1054100.v1.1

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019760
Physical Location & Seq
Reverse (-)
4382015 .. 4383994
1980 bp
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UTR
Exon/CDS
Intron
MD02G1054100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGATTGAGGTATTCACTGCTGAGTATTCTCTAATATGCAAAATCTCAGAGTACAAGAAGCCCTATATAAGCTTCATGGATGGCATAAGAATGGGCTTTGGTGTTGGCCTATCAGGTCACGGTCGCTACTGGATCATTACAGAGAGGACTGTTCTTGCTAATGCCAGAGAATGTAATTTGTTTGTTCCCAGATGTTGGGTTTTCACGTATAGCGGCAAAGAGTCCAGGAGGAGGATCTGTAGGATTTCAACACCATCGGATGCACTGTATGTGGGTCTTGGGACCCATTTTGTCCCATCTCAAAACTTAGCTATTTGTGCCAAAGAATCAAGATGGAGAGGTGGAGAGCTTCAAGTTGATTCGGTGATGCATGTTGCAAGCATCATACGGAGGACACAATTTTTCAATACCGACGCTGGTATTTCACTTTCACTACTGATATTCTTACAATAA

Protein Analysis

151

Amino Acids

17.07

Weight (kDa)

9.27

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 3 - 53 5.3e-17 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019760)

Species Orthologous Gene IDs
malus_domestica MD02G1051100.v1.1 MD02G1054100.v1.1 MD02G1054200.v1.1 MD02G1055400.v1.1 MD02G1055800.v1.1 MD15G1189700.v1.1
pyrus_communis pycom02g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 195
AciI CCGC 1 cut(s) 213
AclWI GGATC 2 cut(s) 140, 242
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 2 cut(s) 195, 231
AgsI TTSAA 3 cut(s) 249, 353, 406
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 3 cut(s) 72, 311, 349
AluI AGCT 3 cut(s) 72, 311, 349
AlwI GGATC 2 cut(s) 140, 242
AoxI GGCC 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 282
BccI CCATC 4 cut(s) 74, 262, 304, 327
BciT130I CCWGG 1 cut(s) 226
BfmI CTRYAG 1 cut(s) 238
BisI GCNGC 1 cut(s) 214
BlsI GCNGC 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 283, 284
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 250, 357, 390
BsaAI YACGTR 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 231
Bse1I ACTGG 1 cut(s) 134
BseBI CCWGG 1 cut(s) 226
BseGI GGATG 2 cut(s) 85, 265
BseLI CCNNNNNNNGG 2 cut(s) 195, 231
BseMII CTCAG 2 cut(s) 12, 60
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 124
BslFI GGGAC 2 cut(s) 278, 295
BslI CCNNNNNNNGG 2 cut(s) 195, 231
BsmFI GGGAC 2 cut(s) 278, 295
BsnI GGCC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 132, 234
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 13, 59
BspLI GGNNCC 2 cut(s) 283, 284
BspPI GGATC 2 cut(s) 140, 242
BsrI ACTGG 1 cut(s) 134
BssMI GATC 2 cut(s) 132, 234
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 3 cut(s) 122, 151, 267
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 3 cut(s) 21, 46, 307
BstF5I GGATG 2 cut(s) 85, 265
BstKTI GATC 2 cut(s) 135, 237
BstMBI GATC 2 cut(s) 132, 234
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 85, 265
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 263
Cac8I GCNNGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 52
CviAII CATG 2 cut(s) 76, 371
CviJI RGCY 6 cut(s) 61, 72, 96, 108, 311, 349
CviKI_1 RGCY 6 cut(s) 61, 72, 96, 108, 311, 349
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 3 cut(s) 21, 46, 307
DpnI GATC 2 cut(s) 134, 236
DpnII GATC 2 cut(s) 132, 234
Eco47I GGWCC 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 224
EcoT22I ATGCAT 1 cut(s) 372
FaeI CATG 2 cut(s) 79, 374
FaiI YATR 9 cut(s) 37, 66, 68, 77, 86, 210, 270, 372, 386
FaqI GGGAC 2 cut(s) 278, 295
FatI CATG 2 cut(s) 75, 370
Fnu4HI GCNGC 1 cut(s) 214
FokI GGATG 2 cut(s) 92, 272
Fsp4HI GCNGC 1 cut(s) 214
GluI GCNGC 1 cut(s) 214
HaeIII GGCC 1 cut(s) 108
HgaI GACGC 1 cut(s) 422
Hin1II CATG 2 cut(s) 79, 374
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 3 cut(s) 221, 326, 359
HphI GGTGA 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 49, 259
Hpy188III TCNNGA 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 416
HpyCH4III ACNGT 3 cut(s) 122, 151, 267
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 4 cut(s) 39, 263, 370, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 3 cut(s) 21, 46, 307
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 79, 374
KflI GGGWCCC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 132, 234
LpnPI CCDG 7 cut(s) 99, 115, 178, 202, 211, 238, 402
LweI GCATC 3 cut(s) 250, 357, 390
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 116
MalI GATC 2 cut(s) 134, 236
MboI GATC 2 cut(s) 132, 234
MflI RGATCY 1 cut(s) 234
MluCI AATT 2 cut(s) 175, 398
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 5 cut(s) 138, 222, 225, 332, 384
Mph1103I ATGCAT 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 2 cut(s) 132, 234
NlaIII CATG 2 cut(s) 79, 374
NlaIV GGNNCC 2 cut(s) 283, 284
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 372
NspI RCATGY 1 cut(s) 374
PfeI GAWTC 2 cut(s) 326, 359
PflMI CCANNNNNTGG 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 1 cut(s) 215
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Ppu21I YACGTR 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 2 cut(s) 283, 284
PspPI GGNCC 1 cut(s) 282
PspPPI RGGWCCY 1 cut(s) 282
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 1 cut(s) 214
Sau3AI GATC 2 cut(s) 132, 234
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 226
SetI ASST 7 cut(s) 12, 74, 118, 209, 313, 343, 351
SfaNI GCATC 3 cut(s) 250, 357, 390
SfcI CTRYAG 1 cut(s) 238
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 2 cut(s) 175, 398
SsiI CCGC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 122, 151, 267
TaiI ACGT 1 cut(s) 209
TasI AATT 2 cut(s) 175, 398
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 2 cut(s) 326, 359
TscAI CASTG 2 cut(s) 22, 270
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 2 cut(s) 22, 270
Van91I CCANNNNNTGG 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 282
XceI RCATGY 1 cut(s) 374
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.