MD02G1063000.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013076
Physical Location & Seq
Reverse (-)
5043282 .. 5044264
983 bp
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UTR
Exon/CDS
Intron
MD02G1063000.v1.1.491

Sequence Viewer

Length: 405 bp
ATGGGGAAGCTAAGTTTGGGCGGGGTGCTGGACAGGTTTTGTCTTTCTTCTGCTGGCTCAGGTTCTTGTTTCTGCATGAACTGGTCGGAACACCTAGATGAGTTCGAAAGAAGTCCGTTGGTAACCGGCGAAAAGGATCAGTTTCTAAAACTGAAGGATGTCGTTGCCGGAAAACAGACGTTGGCCTTTCAGTTGAAGCCCAAGATGGTTATGCTAAGGGTTTCCATGCACTGTTATGGCTGTGCCAAAAAAGTTGAGAAGCATATCTCAAAGATGGAAGGCGTAACTTCTTACAAAGTAGACTTGGAGAGCAAGATGGTGGTTGTGATTGGAGACGTTCTTCCTCTCGAAGTGCTAGAGAGCATCTCCAAGGTTAAAAGTGCTGAGATTTGGAACTCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04978 map04978
Pfam Domains
Protein Families
HMA

Protein Analysis

135

Amino Acids

14.83

Weight (kDa)

8.26

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 73 - 124 1.6e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24450
fragaria_vesca FvH4_1g05260
malus_domestica MD02G1063000.v1.1 MD15G1188800.v1.1
prunus_persica Prupe.7G227800_v2.0.a1
pyrus_communis pycom02g04280 pycom15g16920
rosa_chinensis RchiOBHm_Chr2g0090981
rosa_laevigata RLG00000016163
rosa_multiflora Rmu_sc0000792.1_g000021 Rmu_sc0003305.1_g000002
rosa_roxburghii Rroxscaffold_2G00150710
rosa_rugosa Rorug02G0011400
rosa_samantha Rh2AG057700 Rh2BG055900 Rh2CG058200 Rh2DG057300
rosa_wichuraiana Rw2G005180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 300
AciI CCGC 1 cut(s) 21
AclWI GGATC 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 173
AgsI TTSAA 1 cut(s) 196
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 1 cut(s) 144
AoxI GGCC 1 cut(s) 183
AsuII TTCGAA 1 cut(s) 105
BccI CCATC 3 cut(s) 199, 268, 310
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 2 cut(s) 95, 356
BmsI GCATC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu10I CCTNAGC 2 cut(s) 58, 215
Bpu14I TTCGAA 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 369
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 72, 375
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 388
BshFI GGCC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 126, 168
BsmAI GTCTC 1 cut(s) 327
BsmBI CGTCTC 1 cut(s) 327
BsnI GGCC 1 cut(s) 185
Bsp119I TTCGAA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 136
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 2 cut(s) 71, 376
BspHI TCATGA 1 cut(s) 401
BspPI GGATC 1 cut(s) 144
BspT104I TTCGAA 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 233
BstBI TTCGAA 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 4 cut(s) 11, 58, 215, 384
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 1 cut(s) 136
BstPI GGTNACC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 55
CciI TCATGA 1 cut(s) 401
Cfr10I RCCGGY 1 cut(s) 125
CviAII CATG 3 cut(s) 76, 226, 402
CviJI RGCY 5 cut(s) 10, 57, 185, 199, 240
CviKI_1 RGCY 5 cut(s) 10, 57, 185, 199, 240
DdeI CTNAG 4 cut(s) 11, 58, 215, 384
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 3 cut(s) 79, 229, 405
FaiI YATR 6 cut(s) 77, 212, 227, 237, 264, 403
FatI CATG 3 cut(s) 75, 225, 401
FauI CCCGC 1 cut(s) 14
FblI GTMKAC 1 cut(s) 300
FokI GGATG 1 cut(s) 170
FspBI CTAG 2 cut(s) 95, 356
HaeIII GGCC 1 cut(s) 185
HapII CCGG 2 cut(s) 126, 168
Hin1II CATG 3 cut(s) 79, 229, 405
HpaII CCGG 2 cut(s) 126, 168
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 2 cut(s) 347, 402
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 2 cut(s) 148, 272
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 2 cut(s) 179, 336
HpyCH4V TGCA 2 cut(s) 75, 229
HpyF3I CTNAG 4 cut(s) 11, 58, 215, 384
HpySE526I ACGT 2 cut(s) 179, 336
Hsp92II CATG 3 cut(s) 79, 229, 405
Kzo9I GATC 1 cut(s) 136
LpnPI CCDG 7 cut(s) 14, 19, 39, 45, 67, 139, 181
LweI GCATC 1 cut(s) 372
MaeI CTAG 2 cut(s) 95, 356
MaeII ACGT 2 cut(s) 179, 336
MaeIII GTNAC 2 cut(s) 121, 283
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MboII GAAGA 2 cut(s) 39, 332
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 1 cut(s) 354
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 96, 234
MspI CCGG 2 cut(s) 126, 168
NdeII GATC 1 cut(s) 136
NlaIII CATG 3 cut(s) 79, 229, 405
NspV TTCGAA 1 cut(s) 105
PagI TCATGA 1 cut(s) 401
PspEI GGTNACC 1 cut(s) 121
RseI CAYNNNNRTG 2 cut(s) 96, 234
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 1 cut(s) 136
SetI ASST 7 cut(s) 12, 38, 64, 96, 182, 339, 375
SfaNI GCATC 1 cut(s) 372
SfuI TTCGAA 1 cut(s) 105
SmiMI CAYNNNNRTG 2 cut(s) 96, 234
SsiI CCGC 1 cut(s) 21
SspMI CTAG 2 cut(s) 95, 356
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 2 cut(s) 182, 339
TaqI TCGA 2 cut(s) 105, 348
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 92
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 2 cut(s) 95, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.