MD15G1188800.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013076
Physical Location & Seq
Forward (+)
14907590 .. 14910367
2778 bp
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UTR
Exon/CDS
Intron
MD15G1188800.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGGGAAGCTAAGTATGGGCAGGGTGCTGGACAGGTTTTGTATTTCTTCAACTGGCTCTGGTTCTTGTTTCTGCATGAACTGGTCGGAAAATCTTGACGAATTCGAAAGCAGTCCGTTGGTAACCGGCGAAAAGGATCAGTTTCTAAAACTGAAGGATGTTGTCGCCGGAAAACAGACGTTGGCCTTTCAGTTGAAGCCCAAGATGGTAATGCTAAGGGTTTCCATGCACTGCAATGGCTGTGCCAAAAAAGTTGAGAAACATATCTCAAAGATAGAAGGCGTGACGTCGTACAAAGTAGATTTGGAGAGCAAGATGGTGGTTGTGATCGGAGACGTTCTTCCTTTCGAAGTGCTAGAGAGCATTTCCAAGGTTAAAAATGCAGAGATCTGGAACTCCACATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04978 map04978
Pfam Domains
Protein Families
HMA

Protein Analysis

136

Amino Acids

15.0

Weight (kDa)

8.2

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 73 - 124 2.4e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24450
fragaria_vesca FvH4_1g05260
malus_domestica MD02G1063000.v1.1 MD15G1188800.v1.1
prunus_persica Prupe.7G227800_v2.0.a1
pyrus_communis pycom02g04280 pycom15g16920
rosa_chinensis RchiOBHm_Chr2g0090981
rosa_laevigata RLG00000016163
rosa_multiflora Rmu_sc0000792.1_g000021 Rmu_sc0003305.1_g000002
rosa_roxburghii Rroxscaffold_2G00150710
rosa_rugosa Rorug02G0011400
rosa_samantha Rh2AG057700 Rh2BG055900 Rh2CG058200 Rh2DG057300
rosa_wichuraiana Rw2G005180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 290
AclWI GGATC 1 cut(s) 144
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 1 cut(s) 293
AgsI TTSAA 2 cut(s) 51, 196
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 1 cut(s) 144
AoxI GGCC 1 cut(s) 183
ApoI RAATTY 1 cut(s) 101
AsuII TTCGAA 2 cut(s) 105, 348
BccI CCATC 2 cut(s) 199, 310
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 1 cut(s) 356
BglII AGATCT 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 215
Bpu14I TTCGAA 2 cut(s) 105, 348
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 369
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 58, 86
Bse3DI GCAATG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 241
BseNI ACTGG 2 cut(s) 58, 86
BshFI GGCC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 126, 168
BsmAI GTCTC 1 cut(s) 327
BsmBI CGTCTC 1 cut(s) 327
BsnI GGCC 1 cut(s) 185
Bsp119I TTCGAA 2 cut(s) 105, 348
Bsp143I GATC 3 cut(s) 136, 327, 387
BspANI GGCC 1 cut(s) 185
BspPI GGATC 1 cut(s) 144
BspT104I TTCGAA 2 cut(s) 105, 348
BsrDI GCAATG 1 cut(s) 241
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 58, 86
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 3 cut(s) 136, 327, 387
BssNI GRCGYC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 287
BstBI TTCGAA 2 cut(s) 105, 348
BstDEI CTNAG 2 cut(s) 11, 215
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 3 cut(s) 139, 330, 390
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 3 cut(s) 136, 327, 387
BstNSI RCATGY 1 cut(s) 405
BstPI GGTNACC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 125
Csp6I GTAC 1 cut(s) 292
CviAII CATG 3 cut(s) 76, 226, 402
CviJI RGCY 5 cut(s) 10, 57, 185, 199, 240
CviKI_1 RGCY 5 cut(s) 10, 57, 185, 199, 240
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 2 cut(s) 11, 215
DpnI GATC 3 cut(s) 138, 329, 389
DpnII GATC 3 cut(s) 136, 327, 387
Eco130I CCWWGG 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 3 cut(s) 79, 229, 405
FaiI YATR 5 cut(s) 17, 77, 227, 264, 403
FatI CATG 3 cut(s) 75, 225, 401
FokI GGATG 1 cut(s) 170
FspBI CTAG 1 cut(s) 356
HaeIII GGCC 1 cut(s) 185
HapII CCGG 2 cut(s) 126, 168
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 3 cut(s) 79, 229, 405
HpaII CCGG 2 cut(s) 126, 168
Hpy188I TCNGA 2 cut(s) 88, 332
Hpy188III TCNNGA 2 cut(s) 95, 391
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 148, 272
HpyCH4IV ACGT 3 cut(s) 179, 287, 336
HpyCH4V TGCA 4 cut(s) 75, 229, 234, 383
HpyF3I CTNAG 2 cut(s) 11, 215
HpySE526I ACGT 3 cut(s) 179, 287, 336
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 3 cut(s) 79, 229, 405
Kzo9I GATC 3 cut(s) 136, 327, 387
LpnPI CCDG 9 cut(s) 7, 14, 19, 39, 45, 67, 139, 181, 376
MaeI CTAG 1 cut(s) 356
MaeII ACGT 3 cut(s) 179, 287, 336
MaeIII GTNAC 2 cut(s) 121, 283
MalI GATC 3 cut(s) 138, 329, 389
MboI GATC 3 cut(s) 136, 327, 387
MboII GAAGA 2 cut(s) 39, 332
MflI RGATCY 1 cut(s) 387
MluCI AATT 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 66
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 2 cut(s) 126, 168
NdeII GATC 3 cut(s) 136, 327, 387
NlaIII CATG 3 cut(s) 79, 229, 405
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 1 cut(s) 405
NspV TTCGAA 2 cut(s) 105, 348
PspEI GGTNACC 1 cut(s) 121
PsuI RGATCY 1 cut(s) 387
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 3 cut(s) 136, 327, 387
SetI ASST 6 cut(s) 12, 38, 182, 290, 339, 375
SfuI TTCGAA 2 cut(s) 105, 348
SmiMI CAYNNNNRTG 1 cut(s) 234
Sse9I AATT 1 cut(s) 101
SspMI CTAG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 3 cut(s) 182, 290, 339
TaqI TCGA 2 cut(s) 105, 348
TasI AATT 1 cut(s) 101
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 236
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 92
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 236
XapI RAATTY 1 cut(s) 101
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 356
ZraI GACGTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.