RLG00000016163

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013076
Physical Location & Seq
Forward (+)
4387390 .. 4388021
632 bp
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UTR
Exon/CDS
Intron
RLM00000016163

Sequence Viewer

Length: 405 bp
ATGGGGAAGCTTAGTTTTGGAAAGGTACTAGGCTCTCTTTGTCTTTCTTCTGGATCAAGTTCTTGTTTCTGCATGAACTACTCCGAGGAAAACCAAGACGAGTTTGAGAAAAGTCCCTTGATTCCTGGTGAAAAGGATCAGTTTATGAGACTCAAGGATGTTGTTGCCGGCAAACAAACATTGGCATTTCAACTGAAACCCAAGATGGTAATGTTAAGGGTCTCCATGCACTGCAATGGATGTGCCAGGAAAGTCGAAAAACATATCTCAAAGATGGAAGGGGTGACTTCGTACAAAGTAGACCTGGAAAGCAAGATGGTGGTTGTGATCGGAGATGTTCTGCCTTATGAGGTGGTGCAGAGTGTGTCTAAGGTCAAAAATGCCGAGATTTGGAACTCGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04978 map04978
Pfam Domains
Protein Families
HMA

Protein Analysis

135

Amino Acids

14.9

Weight (kDa)

8.58

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 73 - 124 6.2e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24450
fragaria_vesca FvH4_1g05260
malus_domestica MD02G1063000.v1.1 MD15G1188800.v1.1
prunus_persica Prupe.7G227800_v2.0.a1
pyrus_communis pycom02g04280 pycom15g16920
rosa_chinensis RchiOBHm_Chr2g0090981
rosa_laevigata RLG00000016163
rosa_multiflora Rmu_sc0000792.1_g000021 Rmu_sc0003305.1_g000002
rosa_roxburghii Rroxscaffold_2G00150710
rosa_rugosa Rorug02G0011400
rosa_samantha Rh2AG057700 Rh2BG055900 Rh2CG058200 Rh2DG057300
rosa_wichuraiana Rw2G005180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 300
AclWI GGATC 2 cut(s) 61, 144
AfaI GTAC 2 cut(s) 27, 293
AfiI CCNNNNNNNGG 1 cut(s) 390
AgsI TTSAA 1 cut(s) 191
AjnI CCWGG 3 cut(s) 124, 245, 303
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 2 cut(s) 142, 226
AlwI GGATC 2 cut(s) 61, 144
AsuHPI GGTGA 2 cut(s) 140, 295
BccI CCATC 3 cut(s) 199, 268, 310
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 3 cut(s) 126, 247, 305
BcoDI GTCTC 2 cut(s) 142, 226
BfaI CTAG 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 126, 247, 305
BmrFI CCNGG 3 cut(s) 126, 247, 305
BpuEI CTTGAG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse118I RCCGGY 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 241
BseBI CCWGG 3 cut(s) 126, 247, 305
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 2 cut(s) 163, 245
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMI GCAATG 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 2 cut(s) 142, 226
BsmFI GGGAC 1 cut(s) 99
Bso31I GGTCTC 1 cut(s) 226
Bsp143I GATC 3 cut(s) 53, 136, 327
BspPI GGATC 2 cut(s) 61, 144
BspTNI GGTCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 241
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 3 cut(s) 53, 136, 327
Bst2UI CCWGG 3 cut(s) 126, 247, 305
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 2 cut(s) 11, 369
BstF5I GGATG 2 cut(s) 163, 245
BstKTI GATC 3 cut(s) 56, 139, 330
BstMAI GTCTC 2 cut(s) 142, 226
BstMBI GATC 3 cut(s) 53, 136, 327
BstNI CCWGG 3 cut(s) 126, 247, 305
BstSCI CCNGG 3 cut(s) 124, 245, 303
BtsCI GGATG 2 cut(s) 163, 245
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 167
Csp6I GTAC 2 cut(s) 26, 292
CviAII CATG 2 cut(s) 73, 226
CviJI RGCY 2 cut(s) 10, 33
CviKI_1 RGCY 2 cut(s) 10, 33
CviQI GTAC 2 cut(s) 26, 292
DdeI CTNAG 2 cut(s) 11, 369
DpnI GATC 3 cut(s) 55, 138, 329
DpnII GATC 3 cut(s) 53, 136, 327
Eco31I GGTCTC 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 124, 245, 303
FaeI CATG 2 cut(s) 76, 229
FaiI YATR 5 cut(s) 74, 146, 227, 264, 348
FaqI GGGAC 1 cut(s) 99
FatI CATG 2 cut(s) 72, 225
FblI GTMKAC 1 cut(s) 300
FokI GGATG 2 cut(s) 170, 252
FspBI CTAG 1 cut(s) 29
HapII CCGG 1 cut(s) 168
Hin1II CATG 2 cut(s) 76, 229
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 121, 150
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 140, 295
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 2 cut(s) 85, 332
Hpy188III TCNNGA 1 cut(s) 51
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 272
HpyCH4V TGCA 4 cut(s) 72, 229, 234, 358
HpyF3I CTNAG 2 cut(s) 11, 369
Hsp92II CATG 2 cut(s) 76, 229
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 53, 136, 327
LpnPI CCDG 8 cut(s) 36, 111, 138, 181, 232, 259, 290, 317
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 1 cut(s) 283
MalI GATC 3 cut(s) 55, 138, 329
MboI GATC 3 cut(s) 53, 136, 327
MboII GAAGA 1 cut(s) 39
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 2 cut(s) 79, 343
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 3 cut(s) 126, 247, 305
MvaI CCWGG 3 cut(s) 126, 247, 305
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 3 cut(s) 53, 136, 327
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 2 cut(s) 76, 229
NmuCI GTSAC 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 121
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Psp6I CCWGG 3 cut(s) 124, 245, 303
PspGI CCWGG 3 cut(s) 124, 245, 303
RsaI GTAC 2 cut(s) 27, 293
RsaNI GTAC 2 cut(s) 26, 292
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 215
Sau3AI GATC 3 cut(s) 53, 136, 327
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 3 cut(s) 126, 247, 305
SetI ASST 5 cut(s) 12, 27, 306, 354, 375
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 124, 245, 303
TaqI TCGA 1 cut(s) 255
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 236
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 89
TspRI CASTG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.