Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008624

Physical Location & Seq

Genomic Coordinates Forward (+)

5486786 .. 5497144

Gene Structure 10359 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1067100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGGTGAACAAAACAGCCAAAGCCAATTTGCTAGCAATAGCCACCTCCTTCACTTTCCTTCTCTTTTATCCTACTCCAAAATCCTCCTACCACTCTCTCTTCATTTCCGATTCGCTTTCAGACAATGCCTCCATATCTCACCACCTCTACACCCTCACCCGCCGGCCCCATGTGGCAGGCCTTGAAGCCAACGCCGAAGCTGCAGCTTATGTCCTCTCCGTTTTCGCTTCCTCCAATATCAAATCCCACATTGCCCCTTACCGTGTGGCCTTGCATTATCCGGTGTCACGCTCCTTAACACTCACGCCGTCACCACAAGAATCCCCTATCAGTTTCTGTCTTGAGCAAGAAATCTATGAGGGTGATCCTTATGCTGATGTAGCACATGAAGTCCTGCCCACTTTTCATGCTTATGCAAAGTCAGGAAATGTCACTGCCCCTGTGGTTTATGTCAATTATGGACGTTCGGAGGACTATGCAAGATTAGAACAAATGGGAGTAAATGTTTCGGGTTCGATTGTGTTGGCAAAGTATGGAAAAATTTATAGAGGGGGCATTGTGCAGATTGCATATGAGGCAGGTGCTGTAGGGGTTCTAGTGTATTCAGATAGGAAGGACTATGGCGGTGGTGGAGGTGAGGCAAAGTGGTTTCCGGATAATAAGTGGTTGCCACCAAGCGGGGTTCAAGTGGGATCAGTTTACAATGGACTTGGTGACCCAACAACACCAGGATGGGCAAGTACTGAAGAGTGTGAGAGGCTTTCGGATGATGAGGTGGAGAAGTCAGGGGATGTTCCATTGATTCCTTCCCTTCCAATTTCAGCAGTAGATGGAGAAACCATTTTGAGGTCTATCGGAGGGCCAGTTGCAGATGAGGATTGGCAAGGGAGTGAAGATGCCCCAACTTACAACGTTGGACCAGGTCCCGGGATTGTTCATCTCAGTTACACAGGGAAGCAAGTCATAGGGAGAATTGAGAATGTGATTGGTGTCATAGAAGGAGCAGAGGAACCTGACCGATTGGTCATTCTGGGTAATCACCGGGATGCATGGACATTTGGAGCCGTTGACCCAAATAGTGGCACTGCAGCACTGCTAGAGGTTGCCCAAAGATTAGGGAAGCTGCAGAAAAAAGGGTGGAAACCTCGACGTACGATTGTCTTGTGCAATTGGGATGCAGAGGAATACGGCCTGATAGGATCAACTGAATGGGTAGAAGAAAACAGGGAAATGCTAGCTTCAAGGGCTATTGCATACTTGAATGTTGACAGTGGAGTACACGGTCCAGGATTCTTCGCCTCTGCAACTCCGCAGCTTGATGAACTTCTTAAACAAGCTACTGAACAGGTTAAAGACCCAGATAACTCATCACAAACTCTTTACCAATCGTGGGTTGGTTCCAGAACTTCGCCTAAGATTGACAGGTTGGGAAGTGCGGGATCAGATTTTGCAGCTTTTGTTCACCATCTTGGAATTCCGGCAACTCACATGGCTTTCGGTGAAGGATACCCAGTGTACCATTCAATGTATGATGACTTTATATGGGTGAAGAAATTTGGTGATCCGATGTTTCATAGGCATGTTGCAGTTGCAAGTGTGTGGGGTTTGGTAACTCTTTGGCTCGCAGATGCAGAACTTTTGCCTTTTAATTATCTCTCGTACGCAATGGAGCTACAGACTTATGCGAATGACTTGGAAGATGATATTTCGGACACGAACATAAACCTAACCCCTCTGTTGAAGTCCATTGAAGAGCTCAAAAGAGCAGCCACAGAAATAAACAACGAGAGAAAGGAAATAGAACAAAACAAAGGTTGGCTGTTGACATGGAAGAAAGATCATGTGAAGGTGAGAGAGCTGAATGACAGACTAATGATGGCAGAGCGTGCATTTACAGATCAAGACGGACTATTTGGAAGGTCATGGTATAAGCATTTGATTTATGGGCCTTCGAAGTATGACGACTATGCGTCGGAATCCTTCCCTGGCATATATGATGCTATTGCAAAGGCGAAAATTTCGAACACCGCAGAGTCATGGAAATTTGTACAGCATGAAGTTTATAGGGTCTCCAGAGCTGTGAAACATGCATCACAAATCATCAATGGCGAACTAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0008150 GO:0009888 GO:0010073 GO:0012505 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048507 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

707

Amino Acids

77.97

Weight (kDa)

5.28

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	144 - 230	1e-09	PA domain
Peptidase_M28	PF04389	327 - 513	9.1e-25	Peptidase family M28
TFR_dimer	PF04253	574 - 701	8.1e-36	Transferrin receptor-like dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008624)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19740
fragaria_vesca	FvH4_1g06360 FvH4_1g06360 FvH4_6g31420 FvH4_6g31420 FvH4_6g31420
malus_domestica	MD02G1067100.v1.1 MD02G1067200.v1.1
prunus_persica	Prupe.7G218300_v2.0.a1 Prupe.7G218300_v2.0.a1 Prupe.7G218300_v2.0.a1
pyrus_communis	pycom02g05310
rosa_chinensis	RchiOBHm_Chr2g0092051
rosa_laevigata	RLG00000016272
rosa_multiflora	Rmu_sc0000524.1_g000005
rosa_roxburghii	Rroxscaffold_2G00149510
rosa_rugosa	Rorug02G0022600 Rorug02G0022700
rosa_samantha	Rh2AG068300 Rh2BG067600 Rh2CG068900 Rh2DG067200
rosa_wichuraiana	Rw2G005290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	569
AasI	GACNNNNNNGTC	1 cut(s)	1022
Acc36I	ACCTGC	1 cut(s)	569
AccB7I	CCANNNNNTGG	1 cut(s)	1079
AccIII	TCCGGA	1 cut(s)	652
AciI	CCGC	6 cut(s)	160, 624, 678, 1310, 1436, 2028
AclI	AACGTT	1 cut(s)	912
AclWI	GGATC	5 cut(s)	359, 700, 1207, 1447, 1556
AcsI	RAATTY	5 cut(s)	540, 1473, 1553, 2016, 2042
AcuI	CTGAAG	1 cut(s)	765
AfaI	GTAC	6 cut(s)	742, 1153, 1278, 1517, 1661, 2049
AfiI	CCNNNNNNNGG	5 cut(s)	677, 846, 926, 1079, 1390
AgsI	TTSAA	7 cut(s)	185, 686, 1242, 1261, 1524, 1741, 1751
AhdI	GACNNNNNGTC	1 cut(s)	1969
AjnI	CCWGG	4 cut(s)	727, 919, 1285, 1984
Alw21I	GWGCWC	1 cut(s)	1758
Alw26I	GTCTC	1 cut(s)	2074
AlwI	GGATC	5 cut(s)	359, 700, 1207, 1447, 1556
AlwNI	CAGNNNCTG	2 cut(s)	336, 584
Ama87I	CYCGRG	1 cut(s)	926
Aor13HI	TCCGGA	1 cut(s)	652
AoxI	GGCC	6 cut(s)	164, 178, 267, 860, 1189, 1946
ApeKI	GCWGC	7 cut(s)	200, 203, 1088, 1123, 1312, 1451, 1766
ApoI	RAATTY	5 cut(s)	540, 1473, 1553, 2016, 2042
ArsI	GACNNNNNNTTYG	2 cut(s)	1895, 1927
AspS9I	GGNCC	6 cut(s)	165, 860, 917, 923, 1283, 1946
AsuC2I	CCSGG	3 cut(s)	927, 928, 1043
AsuII	TTCGAA	2 cut(s)	1952, 2021
AsuNHI	GCTAGC	2 cut(s)	31, 1234
AvaI	CYCGRG	1 cut(s)	926
AvaII	GGWCC	3 cut(s)	917, 923, 1283
BanII	GRGCYC	1 cut(s)	1758
Bbv12I	GWGCWC	1 cut(s)	1758
BbvI	GCAGC	7 cut(s)	187, 215, 1100, 1110, 1324, 1463, 1778
BccI	CCATC	4 cut(s)	726, 824, 1473, 1870
BceAI	ACGGC	3 cut(s)	292, 1049, 1204
BciT130I	CCWGG	4 cut(s)	729, 921, 1287, 1986
BciVI	GTATCC	1 cut(s)	1499
BcnI	CCSGG	3 cut(s)	927, 928, 1043
BcoDI	GTCTC	1 cut(s)	2074
BfaI	CTAG	4 cut(s)	32, 596, 1097, 1235
BfmI	CTRYAG	5 cut(s)	201, 585, 1086, 1124, 1673
BfuAI	ACCTGC	1 cut(s)	569
BfuI	GTATCC	1 cut(s)	1499
BisI	GCNGC	7 cut(s)	201, 204, 1089, 1124, 1313, 1452, 1767
BlsI	GCNGC	7 cut(s)	202, 205, 1090, 1125, 1314, 1453, 1768
BmcAI	AGTACT	1 cut(s)	742
Bme1390I	CCNGG	7 cut(s)	729, 921, 927, 928, 1043, 1287, 1986
Bme18I	GGWCC	3 cut(s)	917, 923, 1283
BmeRI	GACNNNNNGTC	1 cut(s)	1969
BmeT110I	CYCGRG	1 cut(s)	926
BmgT120I	GGNCC	6 cut(s)	165, 860, 917, 923, 1283, 1946
BmiI	GGNNCC	5 cut(s)	167, 925, 1011, 1063, 1399
BmrFI	CCNGG	7 cut(s)	729, 921, 927, 928, 1043, 1287, 1986
BmrI	ACTGGG	1 cut(s)	1505
BmsI	GCATC	6 cut(s)	886, 1036, 1165, 1618, 1987, 2099
BmtI	GCTAGC	2 cut(s)	35, 1238
BmuI	ACTGGG	1 cut(s)	1505
BpmI	CTGGAG	1 cut(s)	2056
Bpu14I	TTCGAA	2 cut(s)	1952, 2021
BpuEI	CTTGAG	1 cut(s)	362
BpuMI	CCSGG	3 cut(s)	927, 928, 1043
BsaI	GGTCTC	1 cut(s)	2074
BsaJI	CCNNGG	2 cut(s)	926, 1984
BsaWI	WCCGGW	2 cut(s)	280, 652
BsaXI	ACNNNNNCTCC	6 cut(s)	113, 143, 489, 519, 849, 879
Bsc4I	CCNNNNNNNGG	5 cut(s)	677, 846, 926, 1079, 1390
Bse118I	RCCGGY	1 cut(s)	162
Bse1I	ACTGG	2 cut(s)	863, 1511
Bse3DI	GCAATG	2 cut(s)	249, 1671
BseAI	TCCGGA	1 cut(s)	652
BseBI	CCWGG	4 cut(s)	729, 921, 1287, 1986
BseDI	CCNNGG	2 cut(s)	926, 1984
BseGI	GGATG	5 cut(s)	737, 772, 796, 1051, 1180
BseLI	CCNNNNNNNGG	5 cut(s)	677, 846, 926, 1079, 1390
BseMI	GCAATG	2 cut(s)	249, 1671
BseMII	CTCAG	1 cut(s)	955
BseNI	ACTGG	2 cut(s)	863, 1511
BseXI	GCAGC	7 cut(s)	187, 215, 1100, 1110, 1324, 1463, 1778
BsgI	GTGCAG	1 cut(s)	581
BshFI	GGCC	6 cut(s)	166, 180, 269, 862, 1191, 1948
BsiHKAI	GWGCWC	1 cut(s)	1758
BsiHKCI	CYCGRG	1 cut(s)	926
BsiSI	CCGG	6 cut(s)	163, 281, 653, 927, 1042, 1478
BsiWI	CGTACG	2 cut(s)	1151, 1659
BslFI	GGGAC	1 cut(s)	909
BslI	CCNNNNNNNGG	5 cut(s)	677, 846, 926, 1079, 1390
BsmAI	GTCTC	1 cut(s)	2074
BsmFI	GGGAC	1 cut(s)	909
BsnI	GGCC	6 cut(s)	166, 180, 269, 862, 1191, 1948
Bso31I	GGTCTC	1 cut(s)	2074
BsoBI	CYCGRG	1 cut(s)	926
Bsp119I	TTCGAA	2 cut(s)	1952, 2021
Bsp1286I	GDGCHC	1 cut(s)	1758
Bsp13I	TCCGGA	1 cut(s)	652
Bsp1407I	TGTACA	1 cut(s)	2047
Bsp143I	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
BspACI	CCGC	6 cut(s)	160, 624, 678, 1310, 1436, 2028
BspANI	GGCC	6 cut(s)	166, 180, 269, 862, 1191, 1948
BspCNI	CTCAG	1 cut(s)	954
BspEI	TCCGGA	1 cut(s)	652
BspLI	GGNNCC	5 cut(s)	167, 925, 1011, 1063, 1399
BspMAI	CTGCAG	3 cut(s)	205, 1090, 1128
BspMI	ACCTGC	1 cut(s)	569
BspOI	GCTAGC	2 cut(s)	35, 1238
BspPI	GGATC	5 cut(s)	359, 700, 1207, 1447, 1556
BspQI	GCTCTTC	1 cut(s)	1746
BspT104I	TTCGAA	2 cut(s)	1952, 2021
BspTNI	GGTCTC	1 cut(s)	2074
BsrDI	GCAATG	2 cut(s)	249, 1671
BsrFI	RCCGGY	1 cut(s)	162
BsrGI	TGTACA	1 cut(s)	2047
BsrI	ACTGG	2 cut(s)	863, 1511
BssAI	RCCGGY	1 cut(s)	162
BssECI	CCNNGG	2 cut(s)	926, 1984
BssMI	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
Bst2UI	CCWGG	4 cut(s)	729, 921, 1287, 1986
Bst4CI	ACNGT	3 cut(s)	263, 1271, 1283
Bst6I	CTCTTC	3 cut(s)	104, 741, 1746
BstAPI	GCANNNNNTGC	1 cut(s)	1886
BstAUI	TGTACA	1 cut(s)	2047
BstBI	TTCGAA	2 cut(s)	1952, 2021
BstC8I	GCNNGC	6 cut(s)	33, 164, 178, 1236, 1623, 1887
BstDEI	CTNAG	2 cut(s)	941, 1413
BstEII	GGTNACC	1 cut(s)	713
BstF5I	GGATG	5 cut(s)	737, 772, 796, 1051, 1180
BstKTI	GATC	7 cut(s)	367, 695, 1202, 1442, 1564, 1840, 1900
BstMAI	GTCTC	1 cut(s)	2074
BstMBI	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
BstMWI	GCNNNNNNNGC	5 cut(s)	200, 380, 575, 1244, 1886
BstNI	CCWGG	4 cut(s)	729, 921, 1287, 1986
BstNSI	RCATGY	2 cut(s)	1583, 2090
BstPI	GGTNACC	1 cut(s)	713
BstSCI	CCNGG	7 cut(s)	727, 919, 925, 926, 1041, 1285, 1984
BstSFI	CTRYAG	5 cut(s)	201, 585, 1086, 1124, 1673
BstV1I	GCAGC	7 cut(s)	187, 215, 1100, 1110, 1324, 1463, 1778
BsuI	GTATCC	1 cut(s)	1499
BsuRI	GGCC	6 cut(s)	166, 180, 269, 862, 1191, 1948
BtsCI	GGATG	5 cut(s)	737, 772, 796, 1051, 1180
BtsI	GCAGTG	3 cut(s)	432, 1083, 1091
BtsIMutI	CAGTG	5 cut(s)	432, 1083, 1091, 1276, 1518
BveI	ACCTGC	1 cut(s)	569
Cac8I	GCNNGC	6 cut(s)	33, 164, 178, 1236, 1623, 1887
CaiI	CAGNNNCTG	2 cut(s)	336, 584
Cfr10I	RCCGGY	1 cut(s)	162
Cfr13I	GGNCC	6 cut(s)	165, 860, 917, 923, 1283, 1946
Cfr9I	CCCGGG	1 cut(s)	926
CseI	GACGC	1 cut(s)	1959
CsiI	ACCWGGT	1 cut(s)	919
Csp6I	GTAC	6 cut(s)	741, 1152, 1277, 1516, 1660, 2048
CviQI	GTAC	6 cut(s)	741, 1152, 1277, 1516, 1660, 2048
DdeI	CTNAG	2 cut(s)	941, 1413
DpnI	GATC	7 cut(s)	366, 694, 1201, 1441, 1563, 1839, 1899
DpnII	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
DrdI	GACNNNNNNGTC	1 cut(s)	1022
DriI	GACNNNNNGTC	1 cut(s)	1969
DseDI	GACNNNNNNGTC	1 cut(s)	1022
Eam1104I	CTCTTC	3 cut(s)	104, 741, 1746
Eam1105I	GACNNNNNGTC	1 cut(s)	1969
EarI	CTCTTC	3 cut(s)	104, 741, 1746
Ecl136II	GAGCTC	1 cut(s)	1756
Eco147I	AGGCCT	1 cut(s)	180
Eco24I	GRGCYC	1 cut(s)	1758
Eco31I	GGTCTC	1 cut(s)	2074
Eco47I	GGWCC	3 cut(s)	917, 923, 1283
Eco53kI	GAGCTC	1 cut(s)	1756
Eco57I	CTGAAG	1 cut(s)	765
Eco88I	CYCGRG	1 cut(s)	926
Eco91I	GGTNACC	1 cut(s)	713
EcoICRI	GAGCTC	1 cut(s)	1756
EcoO109I	RGGNCCY	1 cut(s)	923
EcoO65I	GGTNACC	1 cut(s)	713
EcoRI	GAATTC	1 cut(s)	1473
EcoRII	CCWGG	4 cut(s)	727, 919, 1285, 1984
EcoT22I	ATGCAT	2 cut(s)	1051, 2092
EcoT38I	GRGCYC	1 cut(s)	1758
FaqI	GGGAC	1 cut(s)	909
FauI	CCCGC	3 cut(s)	167, 671, 1429
FauNDI	CATATG	1 cut(s)	571
Fnu4HI	GCNGC	7 cut(s)	201, 204, 1089, 1124, 1313, 1452, 1767
FokI	GGATG	5 cut(s)	744, 779, 803, 1058, 1187
FriOI	GRGCYC	1 cut(s)	1758
Fsp4HI	GCNGC	7 cut(s)	201, 204, 1089, 1124, 1313, 1452, 1767
FspBI	CTAG	4 cut(s)	32, 596, 1097, 1235
GluI	GCNGC	7 cut(s)	201, 204, 1089, 1124, 1313, 1452, 1767
GsuI	CTGGAG	1 cut(s)	2056
HaeIII	GGCC	6 cut(s)	166, 180, 269, 862, 1191, 1948
HapII	CCGG	6 cut(s)	163, 281, 653, 927, 1042, 1478
HgaI	GACGC	1 cut(s)	1959
HincII	GTYRAC	3 cut(s)	1069, 1267, 1824
HindII	GTYRAC	3 cut(s)	1069, 1267, 1824
HinfI	GANTC	6 cut(s)	110, 320, 802, 1290, 1976, 2033
HpaII	CCGG	6 cut(s)	163, 281, 653, 927, 1042, 1478
Hpy166II	GTNNAC	8 cut(s)	7, 700, 1069, 1267, 1279, 1462, 1516, 1824
Hpy188III	TCNNGA	6 cut(s)	341, 423, 653, 1401, 1901, 2073
Hpy8I	GTNNAC	8 cut(s)	7, 700, 1069, 1267, 1279, 1462, 1516, 1824
Hpy99I	CGWCG	2 cut(s)	1152, 1975
HpyCH4III	ACNGT	3 cut(s)	263, 1271, 1283
HpyCH4IV	ACGT	3 cut(s)	463, 912, 1150
HpyF10VI	GCNNNNNNNGC	5 cut(s)	200, 380, 575, 1244, 1886
HpyF3I	CTNAG	2 cut(s)	941, 1413
HpySE526I	ACGT	3 cut(s)	463, 912, 1150
Kpn2I	TCCGGA	1 cut(s)	652
KroI	GCCGGC	1 cut(s)	162
KroNI	GCCGGC	1 cut(s)	164
Kzo9I	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
LguI	GCTCTTC	1 cut(s)	1746
LmnI	GCTCC	4 cut(s)	296, 1001, 1061, 1669
Lsp1109I	GCAGC	7 cut(s)	187, 215, 1100, 1110, 1324, 1463, 1778
LweI	GCATC	6 cut(s)	886, 1036, 1165, 1618, 1987, 2099
MabI	ACCWGGT	1 cut(s)	919
MaeI	CTAG	4 cut(s)	32, 596, 1097, 1235
MaeII	ACGT	3 cut(s)	463, 912, 1150
MaeIII	GTNAC	6 cut(s)	285, 309, 430, 713, 944, 1609
MalI	GATC	7 cut(s)	366, 694, 1201, 1441, 1563, 1839, 1899
MboI	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
MboII	GAAGA	9 cut(s)	91, 758, 905, 1229, 1285, 1561, 1709, 1763, 1843
MfeI	CAATTG	1 cut(s)	1168
MhlI	GDGCHC	1 cut(s)	1758
MlyI	GAGTC	1 cut(s)	2042
MmeI	TCCRAC	2 cut(s)	895, 1953
Mph1103I	ATGCAT	2 cut(s)	1051, 2092
MroI	TCCGGA	1 cut(s)	652
MroNI	GCCGGC	1 cut(s)	162
MseI	TTAA	4 cut(s)	296, 1329, 1350, 1647
MslI	CAYNNNNRTG	4 cut(s)	411, 730, 1044, 1578
MspI	CCGG	6 cut(s)	163, 281, 653, 927, 1042, 1478
MspR9I	CCNGG	7 cut(s)	729, 921, 927, 928, 1043, 1287, 1986
MunI	CAATTG	1 cut(s)	1168
MvaI	CCWGG	4 cut(s)	729, 921, 1287, 1986
MwoI	GCNNNNNNNGC	5 cut(s)	200, 380, 575, 1244, 1886
NaeI	GCCGGC	1 cut(s)	164
NciI	CCSGG	3 cut(s)	927, 928, 1043
NdeI	CATATG	1 cut(s)	571
NdeII	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
NgoMIV	GCCGGC	1 cut(s)	162
NheI	GCTAGC	2 cut(s)	31, 1234
NlaIV	GGNNCC	5 cut(s)	167, 925, 1011, 1063, 1399
NmuCI	GTSAC	4 cut(s)	285, 309, 430, 713
NsiI	ATGCAT	2 cut(s)	1051, 2092
NspI	RCATGY	2 cut(s)	1583, 2090
NspV	TTCGAA	2 cut(s)	1952, 2021
PaqCI	CACCTGC	1 cut(s)	569
PceI	AGGCCT	1 cut(s)	180
PciSI	GCTCTTC	1 cut(s)	1746
PdiI	GCCGGC	1 cut(s)	164
PfeI	GAWTC	5 cut(s)	110, 320, 802, 1290, 1976
Pfl23II	CGTACG	2 cut(s)	1151, 1659
PflFI	GACNNNGTC	1 cut(s)	921
PflMI	CCANNNNNTGG	1 cut(s)	1079
PfoI	TCCNGGA	1 cut(s)	1285
PkrI	GCNGC	7 cut(s)	202, 205, 1090, 1125, 1314, 1453, 1768
PleI	GAGTC	1 cut(s)	2041
PpsI	GAGTC	1 cut(s)	2041
PpuMI	RGGWCCY	1 cut(s)	923
Psp124BI	GAGCTC	1 cut(s)	1758
Psp1406I	AACGTT	1 cut(s)	912
Psp5II	RGGWCCY	1 cut(s)	923
Psp6I	CCWGG	4 cut(s)	727, 919, 1285, 1984
PspEI	GGTNACC	1 cut(s)	713
PspGI	CCWGG	4 cut(s)	727, 919, 1285, 1984
PspLI	CGTACG	2 cut(s)	1151, 1659
PspN4I	GGNNCC	5 cut(s)	167, 925, 1011, 1063, 1399
PspPI	GGNCC	6 cut(s)	165, 860, 917, 923, 1283, 1946
PspPPI	RGGWCCY	1 cut(s)	923
PstI	CTGCAG	3 cut(s)	205, 1090, 1128
PstNI	CAGNNNCTG	2 cut(s)	336, 584
PsyI	GACNNNGTC	1 cut(s)	921
RsaI	GTAC	6 cut(s)	742, 1153, 1278, 1517, 1661, 2049
RsaNI	GTAC	6 cut(s)	741, 1152, 1277, 1516, 1660, 2048
RseI	CAYNNNNRTG	4 cut(s)	411, 730, 1044, 1578
SacI	GAGCTC	1 cut(s)	1758
SapI	GCTCTTC	1 cut(s)	1746
SaqAI	TTAA	4 cut(s)	296, 1329, 1350, 1647
SatI	GCNGC	7 cut(s)	201, 204, 1089, 1124, 1313, 1452, 1767
Sau3AI	GATC	7 cut(s)	364, 692, 1199, 1439, 1561, 1837, 1897
Sau96I	GGNCC	6 cut(s)	165, 860, 917, 923, 1283, 1946
ScaI	AGTACT	1 cut(s)	742
SchI	GAGTC	1 cut(s)	2042
ScrFI	CCNGG	7 cut(s)	729, 921, 927, 928, 1043, 1287, 1986
SduI	GDGCHC	1 cut(s)	1758
SexAI	ACCWGGT	1 cut(s)	919
SfaNI	GCATC	6 cut(s)	886, 1036, 1165, 1618, 1987, 2099
SfcI	CTRYAG	5 cut(s)	201, 585, 1086, 1124, 1673
SfuI	TTCGAA	2 cut(s)	1952, 2021
SinI	GGWCC	3 cut(s)	917, 923, 1283
SmaI	CCCGGG	1 cut(s)	928
SmiMI	CAYNNNNRTG	4 cut(s)	411, 730, 1044, 1578
SmlI	CTYRAG	1 cut(s)	341
SmoI	CTYRAG	1 cut(s)	341
SseBI	AGGCCT	1 cut(s)	180
SsiI	CCGC	6 cut(s)	160, 624, 678, 1310, 1436, 2028
SspMI	CTAG	4 cut(s)	32, 596, 1097, 1235
SstI	GAGCTC	1 cut(s)	1758
StuI	AGGCCT	1 cut(s)	180
StyD4I	CCNGG	7 cut(s)	727, 919, 925, 926, 1041, 1285, 1984
TaaI	ACNGT	3 cut(s)	263, 1271, 1283
TaiI	ACGT	3 cut(s)	466, 915, 1153
TaqI	TCGA	4 cut(s)	515, 1147, 1952, 2021
TaqII	GACCGA	1 cut(s)	1032
TatI	WGTACW	3 cut(s)	740, 1276, 2047
TfiI	GAWTC	5 cut(s)	110, 320, 802, 1290, 1976
Tru1I	TTAA	4 cut(s)	296, 1329, 1350, 1647
Tru9I	TTAA	4 cut(s)	296, 1329, 1350, 1647
TscAI	CASTG	5 cut(s)	439, 1090, 1098, 1276, 1518
TseFI	GTSAC	4 cut(s)	285, 309, 430, 713
TseI	GCWGC	7 cut(s)	200, 203, 1088, 1123, 1312, 1451, 1766
Tsp45I	GTSAC	4 cut(s)	285, 309, 430, 713
TspDTI	ATGAA	7 cut(s)	91, 395, 402, 926, 1335, 1562, 2070
TspGWI	ACGGA	2 cut(s)	208, 1920
TspMI	CCCGGG	1 cut(s)	926
TspRI	CASTG	5 cut(s)	439, 1090, 1098, 1276, 1518
Tth111I	GACNNNGTC	1 cut(s)	921
Van91I	CCANNNNNTGG	1 cut(s)	1079
VpaK11BI	GGWCC	3 cut(s)	917, 923, 1283
XapI	RAATTY	5 cut(s)	540, 1473, 1553, 2016, 2042
XceI	RCATGY	2 cut(s)	1583, 2090
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1391
XmaI	CCCGGG	1 cut(s)	926
XspI	CTAG	4 cut(s)	32, 596, 1097, 1235
ZrmI	AGTACT	1 cut(s)	742
Zsp2I	ATGCAT	2 cut(s)	1051, 2092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.