Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0008624

Physical Location & Seq

Genomic Coordinates Forward (+)

5055098 .. 5059075

Gene Structure 3978 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG067200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGATCATCAAAACAGCCACCGCGGCTTTCCTAGCCATACTCACCTCCTTCACCTTCCTCCTCTTTTCTCCGACCCCAAAATCCTCCTACCACTCCCTCTTCATCTCCGACACCCTCTCCTCCAACACCTCCATCTCCCGCCACCTCCACACCCTCACGCGCCGCCCCCACGTCGCCGGAACCCCCGCCAACGCCGACGCCGCCGCCTACGTCCTCTCCATCTTCACCTCCTCCAATCTCCTATCCCGCATCACCCCCTACCACGTGGCCCTCACTTACCCCATCTCCCGCTCCCTAACCCTAACCCCTTCACCTTCATCACCACCCATCACTTTCGACCTCCAACAAGAAACCTACCCCGGCGACCCTTACGCTGACGTGGCAGCCGAGGTCCTCCCCACTTTCCACGCTTTTGCCAAGTCCGGGGATGTCACCGGTCACGTGGTTTACGTCAACTACGGGAGGGTAGAGGACTATGAGACACTGAAGAAAATGGGAGTCAACGTTTCTGGTACAATCGTGTTGGCTAGGTATGGAGAGATTTATAGAGGGAGCATAGTGCAGATTGCTTATGAGCAAGGGGCGGTTGGCGCTTTGGTGTATACGGATAGGAAGGACTACGGCGGAGGAGGCGGAGGGAGGTGGTTTCCGGAGGAGAAGTGGCTGCCGCCGAGTGGGGTACAGGTGGGGACGGTTTACAATGGGATAGGGGACCCGACTACGCCGGGGTGGGCGAGTAGTGAAGAGTGTGAGAGGCTTTCTGATGAGGAAGTGGAGAAGGGTGGGGAATTGACTTTGATACCTTCACTGCCTATTTCTGGTGCTGATGGTGAGACTATTTTGAGGGCCATTGGAGGACAGGTGGCGAATGATGATTGGCAGGGGAGTGGGGATGCCCCAACTTATAGGGTTGGTCCAGGGCCTGGGGTTGTACATCTTAGTTACACTGGGAAGCAAGTTATAGAACAGATTGAGAATGTGATTGGTGTCATAGAAGGTGAAGAAGAACCTGATAGATTTGTCATTCTAGGTAATCACCGGGATGCATGGACATTTGGAGCTGTTGACCCAAATAGTGGCACTGCAGCACTGCTAGAGATTGCTCAAAGATTGGGGAAGTTGCAGGAAAAAGGGTGGAAACCTCGACGCACAATTATCTTGTGCAATTGGGATGCTGAAGAATACGGCCTGATAGGATCAACTGAATGGGTAGAAGAAAACAGAGACATGCTAGCTTCGAGGGCTATTGCTTACTTGAATGTTGACAGTGCAGTATATGCGCCAGGATTCTGGGCCTCTGCCACTCCACAGCTTGATGAACTTCTTAAGCAAGCTACTCAACAGGTCAAAGATCCTGATAACTCATCACAAACTCTCTACCAAGCATGGATTGGTTCCAGCAGTTCTCCTACAATTGGCAGGTTGGGAAGTGGAGAATCTGATTTTGCAGCTTTTGTTCAATATATTGGAATTCCGTCAGCTGACATGGCATTCGGTAAAGGATACCCAGTATACCATTCCATGTATGATGACTTTGTATGGATGGAAAAATTTGGTGATCCTTTCTTTCATAGGCATGTTGCAGTGGCAAGTTTATGGGGTTTGATAGCTCTTTGGCTAGCAGATGCAGAGTTTGTGCCTTTTGATTATACATCCTATGCGCTGGAGCTACAGGTAAGGCAACACTATCTTTCAGTTAACCACTGTATGTCTGTATGTACTTTCGAGATAATTAGACGTGTTAGTTACATTCAGTCAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0008150 GO:0009888 GO:0010073 GO:0012505 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048507 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

587

Amino Acids

64.27

Weight (kDa)

4.94

Isoelectric Point (pI)

45.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	144 - 228	5.4e-07	PA domain
Peptidase_M28	PF04389	326 - 512	4.3e-24	Peptidase family M28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008624)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19740
fragaria_vesca	FvH4_1g06360 FvH4_1g06360 FvH4_6g31420 FvH4_6g31420 FvH4_6g31420
malus_domestica	MD02G1067100.v1.1 MD02G1067200.v1.1
prunus_persica	Prupe.7G218300_v2.0.a1 Prupe.7G218300_v2.0.a1 Prupe.7G218300_v2.0.a1
pyrus_communis	pycom02g05310
rosa_chinensis	RchiOBHm_Chr2g0092051
rosa_laevigata	RLG00000016272
rosa_multiflora	Rmu_sc0000524.1_g000005
rosa_roxburghii	Rroxscaffold_2G00149510
rosa_rugosa	Rorug02G0022600 Rorug02G0022700
rosa_samantha	Rh2AG068300 Rh2BG067600 Rh2CG068900 Rh2DG067200
rosa_wichuraiana	Rw2G005290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1410
AccB7I	CCANNNNNTGG	2 cut(s)	923, 1076
AccBSI	CCGCTC	1 cut(s)	291
AccI	GTMKAC	2 cut(s)	602, 1512
AccII	CGCG	2 cut(s)	23, 160
AccIII	TCCGGA	1 cut(s)	649
AclI	AACGTT	1 cut(s)	504
AclWI	GGATC	3 cut(s)	1204, 1346, 1553
AcsI	RAATTY	2 cut(s)	1470, 1550
AcuI	CTGAAG	2 cut(s)	506, 1197
AcvI	CACGTG	2 cut(s)	265, 442
AcyI	GRCGYC	1 cut(s)	198
AfaI	GTAC	4 cut(s)	514, 681, 933, 1720
AfiI	CCNNNNNNNGG	6 cut(s)	423, 674, 818, 923, 1076, 1415
AflII	CTTAAG	1 cut(s)	1325
AflIII	ACRYGT	1 cut(s)	1738
AgeI	ACCGGT	1 cut(s)	434
AgsI	TTSAA	2 cut(s)	1258, 1460
AjiI	CACGTC	3 cut(s)	172, 379, 1739
AjnI	CCWGG	3 cut(s)	916, 922, 1282
AluBI	AGCT	8 cut(s)	1061, 1235, 1312, 1334, 1451, 1481, 1610, 1669
AluI	AGCT	8 cut(s)	1061, 1235, 1312, 1334, 1451, 1481, 1610, 1669
Alw26I	GTCTC	3 cut(s)	473, 827, 1218
AlwI	GGATC	3 cut(s)	1204, 1346, 1553
AlwNI	CAGNNNCTG	1 cut(s)	923
Aor13HI	TCCGGA	1 cut(s)	649
AoxI	GGCC	5 cut(s)	267, 846, 920, 1186, 1293
ApeKI	GCWGC	4 cut(s)	383, 664, 1085, 1448
ApoI	RAATTY	2 cut(s)	1470, 1550
ArsI	GACNNNNNNTTYG	2 cut(s)	1475, 1507
AsiGI	ACCGGT	1 cut(s)	434
AspLEI	GCGC	4 cut(s)	162, 593, 1282, 1663
AspS9I	GGNCC	7 cut(s)	268, 391, 712, 846, 914, 920, 1293
AsuC2I	CCSGG	4 cut(s)	360, 424, 726, 1040
AsuNHI	GCTAGC	2 cut(s)	1231, 1618
AvaII	GGWCC	3 cut(s)	391, 712, 914
BaeI	ACNNNNGTAYC	2 cut(s)	504, 537
BbrPI	CACGTG	2 cut(s)	265, 442
BbvI	GCAGC	4 cut(s)	395, 651, 1097, 1460
BccI	CCATC	6 cut(s)	140, 227, 290, 335, 821, 1537
BceAI	ACGGC	2 cut(s)	637, 1201
BciT130I	CCWGG	3 cut(s)	918, 924, 1284
BciVI	GTATCC	1 cut(s)	1496
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	4 cut(s)	360, 424, 726, 1040
BcoDI	GTCTC	3 cut(s)	473, 827, 1218
BfaI	CTAG	6 cut(s)	32, 528, 1028, 1094, 1232, 1619
BfmI	CTRYAG	2 cut(s)	1083, 1670
BfoI	RGCGCY	1 cut(s)	594
BfrI	CTTAAG	1 cut(s)	1325
BfuAI	ACCTGC	1 cut(s)	1410
BfuI	GTATCC	1 cut(s)	1496
BglI	GCCNNNNNGGC	1 cut(s)	23
BisI	GCNGC	9 cut(s)	24, 163, 201, 204, 384, 665, 668, 1086, 1449
BlsI	GCNGC	9 cut(s)	25, 164, 202, 205, 385, 666, 669, 1087, 1450
Bme1390I	CCNGG	7 cut(s)	360, 424, 726, 918, 924, 1040, 1284
Bme18I	GGWCC	3 cut(s)	391, 712, 914
BmgBI	CACGTC	3 cut(s)	172, 379, 1739
BmgT120I	GGNCC	7 cut(s)	268, 391, 712, 846, 914, 920, 1293
BmiI	GGNNCC	4 cut(s)	181, 713, 714, 1396
BmrFI	CCNGG	7 cut(s)	360, 424, 726, 918, 924, 1040, 1284
BmrI	ACTGGG	2 cut(s)	957, 1502
BmsI	GCATC	5 cut(s)	258, 883, 1033, 1162, 1615
BmtI	GCTAGC	2 cut(s)	1235, 1622
BmuI	ACTGGG	2 cut(s)	957, 1502
BpmI	CTGGAG	1 cut(s)	1685
BpuMI	CCSGG	4 cut(s)	360, 424, 726, 1040
BsaAI	YACGTR	2 cut(s)	265, 442
BsaHI	GRCGYC	1 cut(s)	198
BsaJI	CCNNGG	7 cut(s)	21, 358, 387, 423, 725, 917, 923
BsaWI	WCCGGW	2 cut(s)	434, 649
Bsc4I	CCNNNNNNNGG	6 cut(s)	423, 674, 818, 923, 1076, 1415
Bse118I	RCCGGY	1 cut(s)	434
Bse1I	ACTGG	2 cut(s)	952, 1508
BseAI	TCCGGA	1 cut(s)	649
BseBI	CCWGG	3 cut(s)	918, 924, 1284
BseDI	CCNNGG	7 cut(s)	21, 358, 387, 423, 725, 917, 923
BseGI	GGATG	6 cut(s)	433, 898, 1048, 1177, 1548, 1652
BseLI	CCNNNNNNNGG	6 cut(s)	423, 674, 818, 923, 1076, 1415
BseNI	ACTGG	2 cut(s)	952, 1508
BseRI	GAGGAG	5 cut(s)	50, 109, 220, 642, 668
BseXI	GCAGC	4 cut(s)	395, 651, 1097, 1460
BsgI	GTGCAG	2 cut(s)	581, 1290
Bsh1236I	CGCG	2 cut(s)	23, 160
BshFI	GGCC	5 cut(s)	269, 848, 922, 1188, 1295
BshTI	ACCGGT	1 cut(s)	434
BsiSI	CCGG	7 cut(s)	177, 360, 423, 435, 650, 725, 1039
BslFI	GGGAC	2 cut(s)	703, 725
BslI	CCNNNNNNNGG	6 cut(s)	423, 674, 818, 923, 1076, 1415
BsmAI	GTCTC	3 cut(s)	473, 827, 1218
BsmFI	GGGAC	2 cut(s)	703, 725
BsmI	GAATGC	1 cut(s)	1490
BsnI	GGCC	5 cut(s)	269, 848, 922, 1188, 1295
Bsp13I	TCCGGA	1 cut(s)	649
Bsp1407I	TGTACA	1 cut(s)	931
Bsp143I	GATC	4 cut(s)	3, 1196, 1351, 1558
BspANI	GGCC	5 cut(s)	269, 848, 922, 1188, 1295
BspEI	TCCGGA	1 cut(s)	649
BspFNI	CGCG	2 cut(s)	23, 160
BspLI	GGNNCC	4 cut(s)	181, 713, 714, 1396
BspMAI	CTGCAG	1 cut(s)	1087
BspMI	ACCTGC	1 cut(s)	1410
BspOI	GCTAGC	2 cut(s)	1235, 1622
BspPI	GGATC	3 cut(s)	1204, 1346, 1553
BspTI	CTTAAG	1 cut(s)	1325
BsrBI	CCGCTC	1 cut(s)	291
BsrFI	RCCGGY	1 cut(s)	434
BsrGI	TGTACA	1 cut(s)	931
BsrI	ACTGG	2 cut(s)	952, 1508
BssAI	RCCGGY	1 cut(s)	434
BssECI	CCNNGG	7 cut(s)	21, 358, 387, 423, 725, 917, 923
BssMI	GATC	4 cut(s)	3, 1196, 1351, 1558
BssNAI	GTATAC	2 cut(s)	603, 1513
BssNI	GRCGYC	1 cut(s)	198
Bst1107I	GTATAC	2 cut(s)	603, 1513
Bst2UI	CCWGG	3 cut(s)	918, 924, 1284
Bst4CI	ACNGT	3 cut(s)	694, 1268, 1706
Bst6I	CTCTTC	2 cut(s)	104, 738
BstACI	GRCGYC	1 cut(s)	198
BstAFI	CTTAAG	1 cut(s)	1325
BstAPI	GCANNNNNTGC	1 cut(s)	1277
BstAUI	TGTACA	1 cut(s)	931
BstBAI	YACGTR	2 cut(s)	265, 442
BstC8I	GCNNGC	3 cut(s)	1233, 1332, 1620
BstDEI	CTNAG	1 cut(s)	938
BstDSI	CCRYGG	1 cut(s)	21
BstF5I	GGATG	6 cut(s)	433, 898, 1048, 1177, 1548, 1652
BstFNI	CGCG	2 cut(s)	23, 160
BstH2I	RGCGCY	1 cut(s)	594
BstHHI	GCGC	4 cut(s)	162, 593, 1282, 1663
BstKTI	GATC	4 cut(s)	6, 1199, 1354, 1561
BstMAI	GTCTC	3 cut(s)	473, 827, 1218
BstMBI	GATC	4 cut(s)	3, 1196, 1351, 1558
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 32, 200, 380, 590, 630, 1241, 1277, 1487
BstNI	CCWGG	3 cut(s)	918, 924, 1284
BstNSI	RCATGY	2 cut(s)	1231, 1580
BstSCI	CCNGG	7 cut(s)	358, 422, 724, 916, 922, 1038, 1282
BstSFI	CTRYAG	2 cut(s)	1083, 1670
BstUI	CGCG	2 cut(s)	23, 160
BstV1I	GCAGC	4 cut(s)	395, 651, 1097, 1460
BstX2I	RGATCY	1 cut(s)	1351
BstXI	CCANNNNNNTGG	1 cut(s)	1290
BstYI	RGATCY	1 cut(s)	1351
BstZ17I	GTATAC	2 cut(s)	603, 1513
BsuI	GTATCC	1 cut(s)	1496
BsuRI	GGCC	5 cut(s)	269, 848, 922, 1188, 1295
BtgI	CCRYGG	1 cut(s)	21
BtrI	CACGTC	3 cut(s)	172, 379, 1739
BtsCI	GGATG	6 cut(s)	433, 898, 1048, 1177, 1548, 1652
BtsI	GCAGTG	4 cut(s)	806, 1080, 1088, 1590
BtsIMutI	CAGTG	8 cut(s)	482, 806, 945, 1080, 1088, 1273, 1590, 1702
BveI	ACCTGC	1 cut(s)	1410
Cac8I	GCNNGC	3 cut(s)	1233, 1332, 1620
CaiI	CAGNNNCTG	1 cut(s)	923
CfoI	GCGC	4 cut(s)	162, 593, 1282, 1663
Cfr10I	RCCGGY	1 cut(s)	434
Cfr13I	GGNCC	7 cut(s)	268, 391, 712, 846, 914, 920, 1293
Cfr42I	CCGCGG	1 cut(s)	24
CseI	GACGC	2 cut(s)	206, 1155
Csp6I	GTAC	4 cut(s)	513, 680, 932, 1719
CspAI	ACCGGT	1 cut(s)	434
CviAII	CATG	6 cut(s)	1047, 1228, 1386, 1486, 1522, 1577
CviQI	GTAC	4 cut(s)	513, 680, 932, 1719
DdeI	CTNAG	1 cut(s)	938
DpnI	GATC	4 cut(s)	5, 1198, 1353, 1560
DpnII	GATC	4 cut(s)	3, 1196, 1351, 1558
Eam1104I	CTCTTC	2 cut(s)	104, 738
EarI	CTCTTC	2 cut(s)	104, 738
EciI	GGCGGA	2 cut(s)	639, 648
Eco47I	GGWCC	3 cut(s)	391, 712, 914
Eco57I	CTGAAG	2 cut(s)	506, 1197
Eco72I	CACGTG	2 cut(s)	265, 442
EcoO109I	RGGNCCY	3 cut(s)	391, 712, 920
EcoRI	GAATTC	1 cut(s)	1470
EcoRII	CCWGG	3 cut(s)	916, 922, 1282
EcoT22I	ATGCAT	1 cut(s)	1048
FaeI	CATG	6 cut(s)	1050, 1231, 1389, 1489, 1525, 1580
FaqI	GGGAC	2 cut(s)	703, 725
FatI	CATG	6 cut(s)	1046, 1227, 1385, 1485, 1521, 1576
FauI	CCCGC	4 cut(s)	146, 193, 254, 296
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	602, 1512
Fnu4HI	GCNGC	9 cut(s)	24, 163, 201, 204, 384, 665, 668, 1086, 1449
FokI	GGATG	6 cut(s)	440, 905, 1055, 1184, 1555, 1639
Fsp4HI	GCNGC	9 cut(s)	24, 163, 201, 204, 384, 665, 668, 1086, 1449
FspBI	CTAG	6 cut(s)	32, 528, 1028, 1094, 1232, 1619
GlaI	GCGC	4 cut(s)	161, 592, 1281, 1662
GluI	GCNGC	9 cut(s)	24, 163, 201, 204, 384, 665, 668, 1086, 1449
GsuI	CTGGAG	1 cut(s)	1685
HaeII	RGCGCY	1 cut(s)	594
HaeIII	GGCC	5 cut(s)	269, 848, 922, 1188, 1295
HapII	CCGG	7 cut(s)	177, 360, 423, 435, 650, 725, 1039
HgaI	GACGC	2 cut(s)	206, 1155
HhaI	GCGC	4 cut(s)	162, 593, 1282, 1663
Hin1I	GRCGYC	1 cut(s)	198
Hin1II	CATG	6 cut(s)	1050, 1231, 1389, 1489, 1525, 1580
Hin6I	GCGC	4 cut(s)	160, 591, 1280, 1661
HinP1I	GCGC	4 cut(s)	160, 591, 1280, 1661
HincII	GTYRAC	6 cut(s)	454, 502, 1066, 1264, 1699, 1758
HindII	GTYRAC	6 cut(s)	454, 502, 1066, 1264, 1699, 1758
HinfI	GANTC	3 cut(s)	498, 1287, 1436
HpaI	GTTAAC	1 cut(s)	1699
HpaII	CCGG	7 cut(s)	177, 360, 423, 435, 650, 725, 1039
Hpy188I	TCNGA	4 cut(s)	72, 109, 763, 1441
Hpy188III	TCNNGA	3 cut(s)	650, 1355, 1726
Hpy99I	CGWCG	3 cut(s)	176, 200, 1149
HpyAV	CCTTC	8 cut(s)	58, 64, 318, 324, 607, 772, 813, 989
HpyCH4III	ACNGT	3 cut(s)	694, 1268, 1706
HpyCH4IV	ACGT	8 cut(s)	171, 210, 264, 378, 441, 450, 504, 1738
HpyCH4V	TGCA	9 cut(s)	562, 1046, 1085, 1123, 1164, 1271, 1448, 1583, 1628
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 32, 200, 380, 590, 630, 1241, 1277, 1487
HpyF3I	CTNAG	1 cut(s)	938
HpySE526I	ACGT	8 cut(s)	171, 210, 264, 378, 441, 450, 504, 1738
Hsp92I	GRCGYC	1 cut(s)	198
Hsp92II	CATG	6 cut(s)	1050, 1231, 1389, 1489, 1525, 1580
HspAI	GCGC	4 cut(s)	160, 591, 1280, 1661
KflI	GGGWCCC	1 cut(s)	712
Kpn2I	TCCGGA	1 cut(s)	649
Ksp22I	TGATCA	1 cut(s)	3
KspAI	GTTAAC	1 cut(s)	1699
KspI	CCGCGG	1 cut(s)	24
Kzo9I	GATC	4 cut(s)	3, 1196, 1351, 1558
LmnI	GCTCC	4 cut(s)	296, 552, 1058, 1666
Lsp1109I	GCAGC	4 cut(s)	395, 651, 1097, 1460
LweI	GCATC	5 cut(s)	258, 883, 1033, 1162, 1615
MaeI	CTAG	6 cut(s)	32, 528, 1028, 1094, 1232, 1619
MaeII	ACGT	8 cut(s)	171, 210, 264, 378, 441, 450, 504, 1738
MaeIII	GTNAC	4 cut(s)	430, 437, 941, 1745
MalI	GATC	4 cut(s)	5, 1198, 1353, 1560
MbiI	CCGCTC	1 cut(s)	291
MboI	GATC	4 cut(s)	3, 1196, 1351, 1558
MboII	GAAGA	8 cut(s)	91, 214, 499, 755, 1013, 1016, 1190, 1226
MfeI	CAATTG	2 cut(s)	1165, 1413
MflI	RGATCY	1 cut(s)	1351
MluCI	AATT	7 cut(s)	788, 1152, 1165, 1413, 1470, 1550, 1731
MlyI	GAGTC	1 cut(s)	507
MmeI	TCCRAC	4 cut(s)	95, 132, 147, 367
Mph1103I	ATGCAT	1 cut(s)	1048
MroI	TCCGGA	1 cut(s)	649
MseI	TTAA	2 cut(s)	1326, 1698
MslI	CAYNNNNRTG	2 cut(s)	1041, 1575
MspA1I	CMGCKG	2 cut(s)	23, 1481
MspCI	CTTAAG	1 cut(s)	1325
MspI	CCGG	7 cut(s)	177, 360, 423, 435, 650, 725, 1039
MspR9I	CCNGG	7 cut(s)	360, 424, 726, 918, 924, 1040, 1284
MunI	CAATTG	2 cut(s)	1165, 1413
Mva1269I	GAATGC	1 cut(s)	1490
MvaI	CCWGG	3 cut(s)	918, 924, 1284
MvnI	CGCG	2 cut(s)	23, 160
MwoI	GCNNNNNNNGC	9 cut(s)	23, 32, 200, 380, 590, 630, 1241, 1277, 1487
NciI	CCSGG	4 cut(s)	360, 424, 726, 1040
NdeII	GATC	4 cut(s)	3, 1196, 1351, 1558
NheI	GCTAGC	2 cut(s)	1231, 1618
NlaIII	CATG	6 cut(s)	1050, 1231, 1389, 1489, 1525, 1580
NlaIV	GGNNCC	4 cut(s)	181, 713, 714, 1396
NmeAIII	GCCGAG	2 cut(s)	412, 696
NmuCI	GTSAC	2 cut(s)	430, 437
NsiI	ATGCAT	1 cut(s)	1048
NspI	RCATGY	2 cut(s)	1231, 1580
PcsI	WCGNNNNNNNCGW	2 cut(s)	384, 447
PctI	GAATGC	1 cut(s)	1490
PfeI	GAWTC	2 cut(s)	1287, 1436
PflMI	CCANNNNNTGG	2 cut(s)	923, 1076
PinAI	ACCGGT	1 cut(s)	434
PkrI	GCNGC	9 cut(s)	25, 164, 202, 205, 385, 666, 669, 1087, 1450
PleI	GAGTC	1 cut(s)	506
PmaCI	CACGTG	2 cut(s)	265, 442
PmlI	CACGTG	2 cut(s)	265, 442
PpsI	GAGTC	1 cut(s)	506
Ppu21I	YACGTR	2 cut(s)	265, 442
PpuMI	RGGWCCY	2 cut(s)	391, 712
Psp1406I	AACGTT	1 cut(s)	504
Psp5II	RGGWCCY	2 cut(s)	391, 712
Psp6I	CCWGG	3 cut(s)	916, 922, 1282
PspCI	CACGTG	2 cut(s)	265, 442
PspGI	CCWGG	3 cut(s)	916, 922, 1282
PspN4I	GGNNCC	4 cut(s)	181, 713, 714, 1396
PspPI	GGNCC	7 cut(s)	268, 391, 712, 846, 914, 920, 1293
PspPPI	RGGWCCY	2 cut(s)	391, 712
PstI	CTGCAG	1 cut(s)	1087
PstNI	CAGNNNCTG	1 cut(s)	923
PsuI	RGATCY	1 cut(s)	1351
PvuII	CAGCTG	1 cut(s)	1481
RsaI	GTAC	4 cut(s)	514, 681, 933, 1720
RsaNI	GTAC	4 cut(s)	513, 680, 932, 1719
RseI	CAYNNNNRTG	2 cut(s)	1041, 1575
SacII	CCGCGG	1 cut(s)	24
SaqAI	TTAA	2 cut(s)	1326, 1698
SatI	GCNGC	9 cut(s)	24, 163, 201, 204, 384, 665, 668, 1086, 1449
Sau3AI	GATC	4 cut(s)	3, 1196, 1351, 1558
Sau96I	GGNCC	7 cut(s)	268, 391, 712, 846, 914, 920, 1293
SchI	GAGTC	1 cut(s)	507
ScrFI	CCNGG	7 cut(s)	360, 424, 726, 918, 924, 1040, 1284
SfaNI	GCATC	5 cut(s)	258, 883, 1033, 1162, 1615
SfcI	CTRYAG	2 cut(s)	1083, 1670
Sfr303I	CCGCGG	1 cut(s)	24
SgrBI	CCGCGG	1 cut(s)	24
SinI	GGWCC	3 cut(s)	391, 712, 914
SmiMI	CAYNNNNRTG	2 cut(s)	1041, 1575
SmlI	CTYRAG	1 cut(s)	1325
SmoI	CTYRAG	1 cut(s)	1325
Sse9I	AATT	7 cut(s)	788, 1152, 1165, 1413, 1470, 1550, 1731
SspMI	CTAG	6 cut(s)	32, 528, 1028, 1094, 1232, 1619
StyD4I	CCNGG	7 cut(s)	358, 422, 724, 916, 922, 1038, 1282
TaaI	ACNGT	3 cut(s)	694, 1268, 1706
TaiI	ACGT	8 cut(s)	174, 213, 267, 381, 444, 453, 507, 1741
TaqI	TCGA	4 cut(s)	336, 1144, 1238, 1725
TasI	AATT	7 cut(s)	788, 1152, 1165, 1413, 1470, 1550, 1731
TatI	WGTACW	2 cut(s)	931, 1718
TauI	GCSGC	5 cut(s)	26, 165, 203, 206, 670
TfiI	GAWTC	2 cut(s)	1287, 1436
Tru1I	TTAA	2 cut(s)	1326, 1698
Tru9I	TTAA	2 cut(s)	1326, 1698
TscAI	CASTG	8 cut(s)	489, 813, 952, 1087, 1095, 1273, 1590, 1709
TseFI	GTSAC	2 cut(s)	430, 437
TseI	GCWGC	4 cut(s)	383, 664, 1085, 1448
Tsp45I	GTSAC	2 cut(s)	430, 437
TspDTI	ATGAA	4 cut(s)	91, 306, 1332, 1559
TspGWI	ACGGA	2 cut(s)	620, 1464
TspRI	CASTG	8 cut(s)	489, 813, 952, 1087, 1095, 1273, 1590, 1709
Van91I	CCANNNNNTGG	2 cut(s)	923, 1076
Vha464I	CTTAAG	1 cut(s)	1325
VpaK11BI	GGWCC	3 cut(s)	391, 712, 914
XapI	RAATTY	2 cut(s)	1470, 1550
XceI	RCATGY	2 cut(s)	1231, 1580
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1388
XmiI	GTMKAC	2 cut(s)	602, 1512
XspI	CTAG	6 cut(s)	32, 528, 1028, 1094, 1232, 1619
Zsp2I	ATGCAT	1 cut(s)	1048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.