MD02G1090100.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015446
Physical Location & Seq
Reverse (-)
7145330 .. 7149840
4511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1090100.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGGGTCACCACCACCACCTCCACAACTCCGGCGATGGCGTCTCGCAGCGAGTCAACAGCCCGAGATTCTCGGGCGCAATGACTCGCCGAGCTCACTCGTTCAAGCGCAACCCCAACGCCGGCGCTGCTGCTGCCTCCAACAATGGAAGTTCTCACAGCAGCAACATCGGAAGTAGCAGTAACACCAACGGCGGCAATGGCGGGTTCGTCGGCGAGTACGAGATTGATCTCCAGCTGAACTCGCCCAGATCTGAAATCGGCGGGAACCCGGTTCCGGCTGATGGGTTTGACTCATTTTTGGAAAGGAAGCAGACCCACAACCATGTAAGCCAGAGAGTTGCGGTGAGAGGGTTCCTGAGGAGGCCAATTGGATCTGTGGTGGCGGACTTGGGGTTGAGGGAGAAGCGGAAGCTTGGCCATTGGATGTTCTTTGCGTTTTGTGGGGTTTGTTTGTTTCTCGGGATTCTGAAGATTTGTGCTACTGGTTGGTTTGGATCTGCTATTGAAACTGCTTCGTCCAACCAGGATGGTGCCGACTCCATGAATCTTTTAAAACGGATGGATCAAAGCTCTCACGAATATGGATATAGGGATGGAGGAAGTCATGTTGAACAAACAATAATGATGGCGTCTGGTGTGAACCGTGTGGTCGTTGAAGAGAAAAGCGCTGAATACACAGGTGTCTGGTCCCGACCGAACAATGAGAATTTCAGTCAGTGCATTGAGTTGCCAAAAACTTACAAACAGCTGGACGCAAAGACCAACGGTTACATTCTTATAAATGCTAATGGCGGCTTGAATCAGATGAGATTTGGGATCTGTGATATGGTTGCTGTTGCTAAGATCTTGAAGGCGACGCTTGTCCTACCTTCACTTGATCACACTAGCTACTGGGCTGATGAGAGTGGTTTTAAAGATCTGTTCAATTGGCAACACTTCATTGAGACTCTAAAGGATGATATCCACATAGTAGAGACATTACCCCCTGCCTATGCTGGAATCGAGCCTTTCAACAAAACACTGATATCTTGGTCTAAGGCTAGTTATTATAAGTCAGAGGTTCTCCCACTGTTGAAGCAGCACAAAGTAATCTATTTCACTCATACGGATTCTCGAATTGCCAACAATGACCTCTCAATTTCCATTCAAAGGCTGAGGTGCCGTGTGAATTACAGGGCATTGAAGTATTCAGCTCCAATAGAAGAACTTGGAAGAACCTTGATTTCTAGAATGCGGAAAAGTGGAGGCCCTTATCTTGCTCTTCATTTGAGGTATGAGAAGGATATGCTTGCATTTACGGGGTGCAGTCATAGTTTGACAGCAGAAGAGGATGAAGAACTACGTAGAATGCGATATGAAGTCAGCCACTGGAAGGAGAAAGAAATTAATGGGACAGAGAGAAGGTTGCTTGGGGGCTGCCCATTGACCCCAAGGGAGACTTCGCTTTTGCTCAGAGGGCTCGGATTTTCATCCAGTACCAGGATTTACTTGGTAGCTGGTGAAGCTTATGGGAATGGAAGTATGCAACATCTTGAGGATGAATTCCCAAACATATTTTCCCATTCCACTCTTGCTAAAGAGGAAGAGCTGAATCCATTCAGGAATCATCAGAACATGTTGGCTGGTATAGACTATGTTGTTGCCTTACAGAGTGACGTTTTTCTTTATACATATGATGGAAACATGGCAAAAGCAGTGCAAGGTCATAGGCGCTTTGAGAACTTTAAGAAGACCATTAATCCAGACAGGATGAATTTTGTGAAACTTGTTGATGAGTTTGACGAAGGAAAAATCTCTTGGAACAAGTTCAGTTCCAAGGTGAAAAAGCTTCACATAGACAGAGTTGGAGCTCCATATCCGAGAGAGGCTGGCGAGTTTCCAAAACTGGAGGAAAGCTTTTACGCAAATCCATATCCAGGCTGTATATGTGAAGCAAGAAGGAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

650

Amino Acids

73.04

Weight (kDa)

8.77

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 257 - 572 1.3e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35570
fragaria_vesca FvH4_1g08590
malus_domestica MD02G1090100.v1.1 MD15G1216700.v1.1
prunus_persica Prupe.7G199900_v2.0.a1
pyrus_communis pycom02g07200
rosa_chinensis RchiOBHm_Chr2g0094811
rosa_laevigata RLG00000016523
rosa_multiflora Rmu_sc0006422.1_g000026 Rmu_sc0011074.1_g000001
rosa_roxburghii Rroxscaffold_2G00146850
rosa_rugosa Rorug02G0047100
rosa_samantha Rh2AG094600 Rh2BG095200 Rh2CG097000 Rh2DG094000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 779, 1050
AccB1I GGYRCC 2 cut(s) 530, 1158
AciI CCGC 8 cut(s) 192, 201, 261, 341, 383, 406, 792, 1234
AclWI GGATC 4 cut(s) 379, 502, 570, 824
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 3 cut(s) 706, 1541, 1753
AcuI CTGAAG 1 cut(s) 488
AcyI GRCGYC 2 cut(s) 39, 629
AfaI GTAC 2 cut(s) 218, 1477
AfeI AGCGCT 1 cut(s) 667
AfiI CCNNNNNNNGG 7 cut(s) 119, 274, 281, 1149, 1372, 1479, 1916
AflIII ACRYGT 1 cut(s) 1614
AjnI CCWGG 3 cut(s) 522, 1478, 1915
AjuI GAANNNNNNNTTGG 6 cut(s) 1423, 1455, 1540, 1572, 1780, 1812
Alw21I GWGCWC 2 cut(s) 94, 1852
Alw26I GTCTC 4 cut(s) 46, 938, 968, 1430
AlwI GGATC 4 cut(s) 379, 502, 570, 824
AlwNI CAGNNNCTG 1 cut(s) 1368
Ama87I CYCGRG 3 cut(s) 61, 70, 458
Aor51HI AGCGCT 1 cut(s) 667
AoxI GGCC 3 cut(s) 362, 415, 1246
ApeKI GCWGC 7 cut(s) 46, 125, 128, 131, 159, 1078, 1416
ApoI RAATTY 3 cut(s) 706, 1541, 1753
ArsI GACNNNNNNTTYG 2 cut(s) 1429, 1461
AseI ATTAAT 2 cut(s) 1386, 1737
Asp700I GAANNNNTTC 2 cut(s) 1595, 1805
AspLEI GCGC 5 cut(s) 77, 108, 125, 668, 1713
AspS9I GGNCC 2 cut(s) 687, 1247
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 3 cut(s) 355, 1511, 1831
AvaI CYCGRG 3 cut(s) 61, 70, 458
AvaII GGWCC 1 cut(s) 687
AxyI CCTNAGG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 417
BanI GGYRCC 2 cut(s) 530, 1158
BanII GRGCYC 3 cut(s) 94, 1461, 1852
BbsI GAAGAC 1 cut(s) 1736
Bbv12I GWGCWC 2 cut(s) 94, 1852
BbvCI CCTCAGC 1 cut(s) 1154
BbvI GCAGC 7 cut(s) 58, 112, 115, 118, 171, 1090, 1403
BccI CCATC 7 cut(s) 29, 275, 521, 553, 587, 619, 1670
BceAI ACGGC 2 cut(s) 205, 1146
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 3 cut(s) 524, 1480, 1917
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 4 cut(s) 46, 938, 968, 1430
BfaI CTAG 3 cut(s) 885, 1041, 1227
BfoI RGCGCY 3 cut(s) 126, 669, 1714
BglII AGATCT 3 cut(s) 248, 843, 916
BisI GCNGC 9 cut(s) 47, 126, 129, 132, 160, 193, 793, 1079, 1417
BlsI GCNGC 9 cut(s) 48, 127, 130, 133, 161, 194, 794, 1080, 1418
Bme1390I CCNGG 4 cut(s) 269, 524, 1480, 1917
Bme18I GGWCC 1 cut(s) 687
BmeT110I CYCGRG 3 cut(s) 61, 70, 458
BmgT120I GGNCC 2 cut(s) 687, 1247
BmiI GGNNCC 6 cut(s) 266, 273, 353, 532, 689, 1160
BmrFI CCNGG 4 cut(s) 269, 524, 1480, 1917
BmrI ACTGGG 1 cut(s) 901
BmuI ACTGGG 1 cut(s) 901
BoxI GACNNNNGTC 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 1736
BpmI CTGGAG 2 cut(s) 215, 1907
Bpu10I CCTNAGC 1 cut(s) 1154
BpuEI CTTGAG 1 cut(s) 1553
BpuMI CCSGG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 1343
BsaBI GATNNNNATC 1 cut(s) 1468
BsaHI GRCGYC 2 cut(s) 39, 629
BsaJI CCNNGG 2 cut(s) 1430, 1815
Bsc4I CCNNNNNNNGG 7 cut(s) 119, 274, 281, 1149, 1372, 1479, 1916
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 5 cut(s) 487, 896, 1373, 1473, 1890
Bse21I CCTNAGG 1 cut(s) 356
Bse3DI GCAATG 2 cut(s) 84, 202
Bse8I GATNNNNATC 1 cut(s) 1468
BseBI CCWGG 3 cut(s) 524, 1480, 1917
BseDI CCNNGG 2 cut(s) 1430, 1815
BseGI GGATG 9 cut(s) 429, 532, 564, 598, 961, 1336, 1469, 1543, 1755
BseJI GATNNNNATC 1 cut(s) 1468
BseLI CCNNNNNNNGG 7 cut(s) 119, 274, 281, 1149, 1372, 1479, 1916
BseMI GCAATG 2 cut(s) 84, 202
BseMII CTCAG 3 cut(s) 347, 1145, 1465
BseNI ACTGG 5 cut(s) 487, 896, 1373, 1473, 1890
BseRI GAGGAG 1 cut(s) 373
BseXI GCAGC 7 cut(s) 58, 112, 115, 118, 171, 1090, 1403
BsgI GTGCAG 1 cut(s) 1324
Bsh1285I CGRYCG 1 cut(s) 695
BshFI GGCC 3 cut(s) 364, 417, 1248
BshNI GGYRCC 2 cut(s) 530, 1158
BsiEI CGRYCG 1 cut(s) 695
BsiHKAI GWGCWC 2 cut(s) 94, 1852
BsiHKCI CYCGRG 3 cut(s) 61, 70, 458
BsiSI CCGG 4 cut(s) 30, 120, 269, 275
BslFI GGGAC 2 cut(s) 673, 1405
BslI CCNNNNNNNGG 7 cut(s) 119, 274, 281, 1149, 1372, 1479, 1916
BsmAI GTCTC 4 cut(s) 46, 938, 968, 1430
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 673, 1405
BsmI GAATGC 2 cut(s) 1236, 1353
BsnI GGCC 3 cut(s) 364, 417, 1248
BsoBI CYCGRG 3 cut(s) 61, 70, 458
Bsp1286I GDGCHC 3 cut(s) 94, 1461, 1852
Bsp143I GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
BspACI CCGC 8 cut(s) 192, 201, 261, 341, 383, 406, 792, 1234
BspANI GGCC 3 cut(s) 364, 417, 1248
BspCNI CTCAG 3 cut(s) 348, 1146, 1464
BspLI GGNNCC 6 cut(s) 266, 273, 353, 532, 689, 1160
BspPI GGATC 4 cut(s) 379, 502, 570, 824
BspQI GCTCTTC 2 cut(s) 1266, 1578
BspT107I GGYRCC 2 cut(s) 530, 1158
BsrDI GCAATG 2 cut(s) 84, 202
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 5 cut(s) 487, 896, 1373, 1473, 1890
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 1430, 1815
BssMI GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
BssNI GRCGYC 2 cut(s) 39, 629
BssT1I CCWWGG 2 cut(s) 1430, 1815
Bst2UI CCWGG 3 cut(s) 524, 1480, 1917
Bst4CI ACNGT 3 cut(s) 644, 767, 1071
Bst6I CTCTTC 4 cut(s) 651, 1266, 1320, 1578
BstACI GRCGYC 2 cut(s) 39, 629
BstBAI YACGTR 1 cut(s) 1343
BstC8I GCNNGC 3 cut(s) 121, 1290, 1870
BstDEI CTNAG 5 cut(s) 356, 840, 1035, 1154, 1451
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 9 cut(s) 429, 532, 564, 598, 961, 1336, 1469, 1543, 1755
BstH2I RGCGCY 3 cut(s) 126, 669, 1714
BstHHI GCGC 5 cut(s) 77, 108, 125, 668, 1713
BstKTI GATC 9 cut(s) 229, 251, 374, 497, 565, 819, 846, 880, 919
BstMAI GTCTC 4 cut(s) 46, 938, 968, 1430
BstMBI GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
BstMCI CGRYCG 1 cut(s) 695
BstMWI GCNNNNNNNGC 6 cut(s) 125, 131, 198, 241, 1456, 1502
BstNI CCWGG 3 cut(s) 524, 1480, 1917
BstNSI RCATGY 1 cut(s) 1618
BstPAI GACNNNNGTC 1 cut(s) 860
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 4 cut(s) 267, 522, 1478, 1915
BstSNI TACGTA 1 cut(s) 1343
BstV1I GCAGC 7 cut(s) 58, 112, 115, 118, 171, 1090, 1403
BstV2I GAAGAC 1 cut(s) 1736
BstX2I RGATCY 6 cut(s) 248, 371, 494, 816, 843, 916
BstYI RGATCY 6 cut(s) 248, 371, 494, 816, 843, 916
Bsu36I CCTNAGG 1 cut(s) 356
BsuRI GGCC 3 cut(s) 364, 417, 1248
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 9 cut(s) 429, 532, 564, 598, 961, 1336, 1469, 1543, 1755
BtsI GCAGTG 1 cut(s) 1701
BtsIMutI CAGTG 5 cut(s) 722, 1019, 1067, 1366, 1701
Cac8I GCNNGC 3 cut(s) 121, 1290, 1870
CaiI CAGNNNCTG 1 cut(s) 1368
CfoI GCGC 5 cut(s) 77, 108, 125, 668, 1713
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 687, 1247
CseI GACGC 4 cut(s) 28, 618, 761, 865
Csp6I GTAC 2 cut(s) 217, 1476
CviAII CATG 5 cut(s) 323, 541, 605, 1615, 1684
CviQI GTAC 2 cut(s) 217, 1476
DdeI CTNAG 5 cut(s) 356, 840, 1035, 1154, 1451
DpnI GATC 9 cut(s) 228, 250, 373, 496, 564, 818, 845, 879, 918
DpnII GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
DraI TTTAAA 2 cut(s) 552, 913
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 4 cut(s) 651, 1266, 1320, 1578
EarI CTCTTC 4 cut(s) 651, 1266, 1320, 1578
EciI GGCGGA 1 cut(s) 398
Ecl136II GAGCTC 2 cut(s) 92, 1850
Eco105I TACGTA 1 cut(s) 1343
Eco130I CCWWGG 2 cut(s) 1430, 1815
Eco24I GRGCYC 3 cut(s) 94, 1461, 1852
Eco32I GATATC 2 cut(s) 961, 1026
Eco47I GGWCC 1 cut(s) 687
Eco47III AGCGCT 1 cut(s) 667
Eco53kI GAGCTC 2 cut(s) 92, 1850
Eco57I CTGAAG 1 cut(s) 488
Eco81I CCTNAGG 1 cut(s) 356
Eco88I CYCGRG 3 cut(s) 61, 70, 458
Eco91I GGTNACC 1 cut(s) 5
EcoICRI GAGCTC 2 cut(s) 92, 1850
EcoO109I RGGNCCY 1 cut(s) 1247
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 1541
EcoRII CCWGG 3 cut(s) 522, 1478, 1915
EcoRV GATATC 2 cut(s) 961, 1026
EcoT14I CCWWGG 2 cut(s) 1430, 1815
EcoT38I GRGCYC 3 cut(s) 94, 1461, 1852
ErhI CCWWGG 2 cut(s) 1430, 1815
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 5 cut(s) 326, 544, 608, 1618, 1687
FalI AAGNNNNNCTT 2 cut(s) 1423, 1455
FaqI GGGAC 2 cut(s) 673, 1405
FatI CATG 5 cut(s) 322, 540, 604, 1614, 1683
FauI CCCGC 2 cut(s) 194, 254
FauNDI CATATG 1 cut(s) 1672
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 9 cut(s) 47, 126, 129, 132, 160, 193, 793, 1079, 1417
FokI GGATG 9 cut(s) 436, 539, 571, 605, 968, 1343, 1456, 1550, 1762
FriOI GRGCYC 3 cut(s) 94, 1461, 1852
Fsp4HI GCNGC 9 cut(s) 47, 126, 129, 132, 160, 193, 793, 1079, 1417
FspBI CTAG 3 cut(s) 885, 1041, 1227
GlaI GCGC 5 cut(s) 76, 107, 124, 667, 1712
GluI GCNGC 9 cut(s) 47, 126, 129, 132, 160, 193, 793, 1079, 1417
GsuI CTGGAG 2 cut(s) 215, 1907
HaeII RGCGCY 3 cut(s) 126, 669, 1714
HaeIII GGCC 3 cut(s) 364, 417, 1248
HapII CCGG 4 cut(s) 30, 120, 269, 275
HgaI GACGC 4 cut(s) 28, 618, 761, 865
HhaI GCGC 5 cut(s) 77, 108, 125, 668, 1713
Hin1I GRCGYC 2 cut(s) 39, 629
Hin1II CATG 5 cut(s) 326, 544, 608, 1618, 1687
Hin6I GCGC 5 cut(s) 75, 106, 123, 666, 1711
HinP1I GCGC 5 cut(s) 75, 106, 123, 666, 1711
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 4 cut(s) 410, 1503, 1826, 1894
HpaII CCGG 4 cut(s) 30, 120, 269, 275
HphI GGTGA 3 cut(s) 355, 1511, 1831
Hpy166II GTNNAC 2 cut(s) 55, 640
Hpy188I TCNGA 9 cut(s) 170, 253, 468, 804, 1057, 1454, 1463, 1611, 1860
Hpy8I GTNNAC 2 cut(s) 55, 640
Hpy99I CGWCG 2 cut(s) 212, 859
HpyAV CCTTC 7 cut(s) 844, 879, 1273, 1366, 1395, 1778, 1932
HpyCH4III ACNGT 3 cut(s) 644, 767, 1071
HpyCH4IV ACGT 2 cut(s) 1342, 1656
HpyCH4V TGCA 5 cut(s) 720, 1292, 1305, 1525, 1699
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 131, 198, 241, 1456, 1502
HpyF3I CTNAG 5 cut(s) 356, 840, 1035, 1154, 1451
HpySE526I ACGT 2 cut(s) 1342, 1656
Hsp92I GRCGYC 2 cut(s) 39, 629
Hsp92II CATG 5 cut(s) 326, 544, 608, 1618, 1687
HspAI GCGC 5 cut(s) 75, 106, 123, 666, 1711
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
LguI GCTCTTC 2 cut(s) 1266, 1578
LmnI GCTCC 3 cut(s) 1198, 1847, 1855
Lsp1109I GCAGC 7 cut(s) 58, 112, 115, 118, 171, 1090, 1403
MaeI CTAG 3 cut(s) 885, 1041, 1227
MaeII ACGT 2 cut(s) 1342, 1656
MaeIII GTNAC 4 cut(s) 5, 179, 767, 1652
MalI GATC 9 cut(s) 228, 250, 373, 496, 564, 818, 845, 879, 918
MboI GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
MboII GAAGA 9 cut(s) 481, 668, 1214, 1224, 1253, 1337, 1346, 1595, 1741
MfeI CAATTG 2 cut(s) 366, 925
MflI RGATCY 6 cut(s) 248, 371, 494, 816, 843, 916
MhlI GDGCHC 3 cut(s) 94, 1461, 1852
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 9 cut(s) 366, 706, 925, 1116, 1137, 1168, 1383, 1541, 1753
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 5 cut(s) 60, 76, 284, 530, 940
MmeI TCCRAC 3 cut(s) 162, 543, 1825
Mox20I TGGCCA 1 cut(s) 417
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 1595, 1805
MscI TGGCCA 1 cut(s) 417
MseI TTAA 5 cut(s) 551, 912, 1386, 1725, 1737
MslI CAYNNNNRTG 2 cut(s) 321, 579
Msp20I TGGCCA 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 235, 748
MspI CCGG 4 cut(s) 30, 120, 269, 275
MspR9I CCNGG 4 cut(s) 269, 524, 1480, 1917
MunI CAATTG 2 cut(s) 366, 925
Mva1269I GAATGC 2 cut(s) 1236, 1353
MvaI CCWGG 3 cut(s) 524, 1480, 1917
MwoI GCNNNNNNNGC 6 cut(s) 125, 131, 198, 241, 1456, 1502
NaeI GCCGGC 1 cut(s) 121
NciI CCSGG 1 cut(s) 269
NdeI CATATG 1 cut(s) 1672
NdeII GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 5 cut(s) 326, 544, 608, 1618, 1687
NlaIV GGNNCC 6 cut(s) 266, 273, 353, 532, 689, 1160
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 2 cut(s) 5, 1652
NspI RCATGY 1 cut(s) 1618
PciI ACATGT 1 cut(s) 1614
PciSI GCTCTTC 2 cut(s) 1266, 1578
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 1348
PctI GAATGC 2 cut(s) 1236, 1353
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 1595, 1805
PfeI GAWTC 8 cut(s) 66, 463, 544, 799, 999, 1109, 1591, 1603
PkrI GCNGC 9 cut(s) 48, 127, 130, 133, 161, 194, 794, 1080, 1418
PleI GAGTC 5 cut(s) 59, 76, 284, 530, 940
PpsI GAGTC 5 cut(s) 59, 76, 284, 530, 940
Ppu21I YACGTR 1 cut(s) 1343
PscI ACATGT 1 cut(s) 1614
PshAI GACNNNNGTC 1 cut(s) 860
PshBI ATTAAT 2 cut(s) 1386, 1737
PsiI TTATAA 2 cut(s) 779, 1050
Psp124BI GAGCTC 2 cut(s) 94, 1852
Psp6I CCWGG 3 cut(s) 522, 1478, 1915
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 522, 1478, 1915
PspN4I GGNNCC 6 cut(s) 266, 273, 353, 532, 689, 1160
PspPI GGNCC 2 cut(s) 687, 1247
PstNI CAGNNNCTG 1 cut(s) 1368
PsuI RGATCY 6 cut(s) 248, 371, 494, 816, 843, 916
PvuII CAGCTG 2 cut(s) 235, 748
RsaI GTAC 2 cut(s) 218, 1477
RsaNI GTAC 2 cut(s) 217, 1476
RseI CAYNNNNRTG 2 cut(s) 321, 579
SacI GAGCTC 2 cut(s) 94, 1852
SapI GCTCTTC 2 cut(s) 1266, 1578
SaqAI TTAA 5 cut(s) 551, 912, 1386, 1725, 1737
SatI GCNGC 9 cut(s) 47, 126, 129, 132, 160, 193, 793, 1079, 1417
Sau3AI GATC 9 cut(s) 226, 248, 371, 494, 562, 816, 843, 877, 916
Sau96I GGNCC 2 cut(s) 687, 1247
SchI GAGTC 5 cut(s) 60, 76, 284, 530, 940
ScrFI CCNGG 4 cut(s) 269, 524, 1480, 1917
SduI GDGCHC 3 cut(s) 94, 1461, 1852
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 1 cut(s) 687
SmiMI CAYNNNNRTG 2 cut(s) 321, 579
SmlI CTYRAG 1 cut(s) 1532
SmoI CTYRAG 1 cut(s) 1532
SnaBI TACGTA 1 cut(s) 1343
Sse9I AATT 9 cut(s) 366, 706, 925, 1116, 1137, 1168, 1383, 1541, 1753
SsiI CCGC 8 cut(s) 192, 201, 261, 341, 383, 406, 792, 1234
SspMI CTAG 3 cut(s) 885, 1041, 1227
SstI GAGCTC 2 cut(s) 94, 1852
StyD4I CCNGG 4 cut(s) 267, 522, 1478, 1915
StyI CCWWGG 2 cut(s) 1430, 1815
TaaI ACNGT 3 cut(s) 644, 767, 1071
TaiI ACGT 2 cut(s) 1345, 1659
TaqI TCGA 2 cut(s) 1002, 1114
TaqII GACCGA 1 cut(s) 709
TasI AATT 9 cut(s) 366, 706, 925, 1116, 1137, 1168, 1383, 1541, 1753
TauI GCSGC 2 cut(s) 195, 795
TfiI GAWTC 8 cut(s) 66, 463, 544, 799, 999, 1109, 1591, 1603
Tru1I TTAA 5 cut(s) 551, 912, 1386, 1725, 1737
Tru9I TTAA 5 cut(s) 551, 912, 1386, 1725, 1737
TscAI CASTG 5 cut(s) 722, 1026, 1074, 1373, 1701
TseFI GTSAC 2 cut(s) 5, 1652
TseI GCWGC 7 cut(s) 46, 125, 128, 131, 159, 1078, 1416
Tsp45I GTSAC 2 cut(s) 5, 1652
TspDTI ATGAA 8 cut(s) 557, 928, 1253, 1347, 1371, 1458, 1554, 1766
TspGWI ACGGA 2 cut(s) 571, 1121
TspRI CASTG 5 cut(s) 722, 1026, 1074, 1373, 1701
VpaK11BI GGWCC 1 cut(s) 687
VspI ATTAAT 2 cut(s) 1386, 1737
XapI RAATTY 3 cut(s) 706, 1541, 1753
XbaI TCTAGA 1 cut(s) 1226
XceI RCATGY 1 cut(s) 1618
XcmI CCANNNNNNNNNTGG 1 cut(s) 1486
XmnI GAANNNNTTC 2 cut(s) 1595, 1805
XspI CTAG 3 cut(s) 885, 1041, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.