pycom02g07200

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015446
Physical Location & Seq
Reverse (-)
5043943 .. 5047229
3287 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07200.1

Sequence Viewer

Length: 1947 bp
ATGGGTCACCACCACCACCTTCACAACGCCAGCGATGGCGTCTCGCAGCGAGTCAACAGCCCGAGATTCTCGGGCGCAATGACTCGCCGAGCTCACTCGTTCAAGCGCAACCCCAATGCCAGCGCTGCTGCCGCCAACAATGGAAGTTCTCACAGCAGCAACATCGGAAGCAGCAGTAACACCAACGGCGGCAATGGCGGGTTCGTCGGCGAGTACGAGATTGATCTGCAGCTGAACTCGCCCAGATCTGAAATCGGTGGGAACCCGGTTCCGGCTGATGGGTTTGACTCGTTTTTGGAAAGGAAGCAGACCCACAACCATGTGAGCCAGAGAGTTGCGGTGAGGGGGTTCCTGAGGAGGCCAATTGGATCTGTGGTGGTGGACTTGGGGTTGAGGGAGAAGAGGAAGCTTGGCCATTGGATGTTCTTCGCGTTTTGTGGGGTTTGTTTGTTTCTCGGGATTCTGAAGATTTGTGCTACTGGTTGGTTTGGATCTGCTATTGAAACTGCTAGGTCCAACCAGGATGGTTCCGACTCCATGAATCTTTTAAAACGGATGGATCAAGGCTCTCACGAATATGGATATAGTGATGGAGGAAGTCATGTTGAACAAACAATAATGATGGCGTCGGGTGTAAACCGTGTGGTCGTTGAAGAGAAAAGCGCTGAATACACAGGTATCTGGTCCCGACCGAACAGTGAGAATTTCAGTCAGTGCATTGAGTTGCCAAAAACTCATAAACAGCTGGACGCAAAGACCAACGGTTACATTCTTATAAATGCTAATGGCGGCTTGAATCAGATGAGATTTGGGATCTGTGATATGGTTGCTGTTGCTAAGATTATGAAGGCGACGCTTGTCCTCCCTTCACTTGATCACACTAACTACTGGGCTGATGAGAGTGGTTTTAAAGATCTGTTCAATTGGCAACACTTCATTGAGACTCTAAAGGATGATATCCACATAGTAGAGACATTACCCCCTGCCTATGCTGGAATCGAGCCTTTCAACAAAACACTGATATCTTGGTCTAAGGCTAGTTATTATAAGTCAGAGGTTCTCCCACTGTTGAAGCAGCACAAAGTAATCTATTTCACTCATACGGATTCTCGAATTGCCAACAATGACCTCTCAATTTCCATTCAAAGGCTGAGGTGCCGTGTGAATTACAGGGCATTGAAGTATTCAGCTCCAATAGAAGAACTTGGAAGAACCTTGATTTCTAGAATGCGGAAAAATGGAGGCCCTTATCTTGCTCTTCATTTGAGGTATGAGAAGGATATGCTTGCATTTACGGGCTGCAGTCATAGTTTGACAGCAGAGGAGGATGAAGAACTACGTAGAATGCGATACGAAGTCAGCCACTGGAAGGAGAAAGAAATTAATGGGACAGAGAGAAGGTTGCTTGGGGGCTGCCCATTGACCCCAAGGGAGACTTCGCTTTTGCTCAGAGGGCTCGGATTTCCATCCAGTACCAGGATTTACTTGGTAGCTGGTGAAGCTTATGGGAATGGAAGTATGCAACATCTTGAGGATGAATTCCCGAACATATTTTCCCATTCCACTCTCGCTAAAGAGGAAGAGCTGAATCCATTCAGGAATCATCAGAACATGTTGGCTGGTATAGACTATGTTGTTGCCTTACAGAGTGATGTTTTTCTTTATACATATGACGGAAACATGGCAAAAGCAGTGCAAGGTCATAGGCGCTTTGAGAACTTTAAGAAGACCATTAATCCAGACAGGATGAATTTTGTAAAACTTGTCGATGACTTTGACGAAGGAAAAATCTCTTGGAACAAATTCAGTTCCAAGGTGAAAAAGCTTCACATCGACAGAGTTGGAGCTCCATATCCGAGAGAGGCTGGCGAGTTTCCAAAACTGGAGGAAAGCTTTTACGCAAATCCATATCCAGGCTGTATATGTGAAGCAAGAAGGAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

649

Amino Acids

73.04

Weight (kDa)

8.77

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 256 - 571 2e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35570
fragaria_vesca FvH4_1g08590
malus_domestica MD02G1090100.v1.1 MD15G1216700.v1.1
prunus_persica Prupe.7G199900_v2.0.a1
pyrus_communis pycom02g07200
rosa_chinensis RchiOBHm_Chr2g0094811
rosa_laevigata RLG00000016523
rosa_multiflora Rmu_sc0006422.1_g000026 Rmu_sc0011074.1_g000001
rosa_roxburghii Rroxscaffold_2G00146850
rosa_rugosa Rorug02G0047100
rosa_samantha Rh2AG094600 Rh2BG095200 Rh2CG097000 Rh2DG094000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 776, 1047
AccB1I GGYRCC 1 cut(s) 1155
AccII CGCG 1 cut(s) 431
AciI CCGC 6 cut(s) 132, 189, 198, 338, 789, 1231
AclWI GGATC 4 cut(s) 376, 499, 567, 821
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 4 cut(s) 703, 1538, 1750, 1802
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 2 cut(s) 39, 626
AfaI GTAC 2 cut(s) 215, 1474
AfeI AGCGCT 2 cut(s) 124, 664
AfiI CCNNNNNNNGG 6 cut(s) 271, 278, 1146, 1369, 1476, 1913
AflIII ACRYGT 1 cut(s) 1611
AjnI CCWGG 3 cut(s) 519, 1475, 1912
AjuI GAANNNNNNNTTGG 4 cut(s) 1420, 1452, 1777, 1809
AloI GAACNNNNNNTCC 2 cut(s) 1538, 1570
Alw21I GWGCWC 2 cut(s) 94, 1849
Alw26I GTCTC 4 cut(s) 46, 935, 965, 1427
AlwI GGATC 4 cut(s) 376, 499, 567, 821
AlwNI CAGNNNCTG 1 cut(s) 1365
Ama87I CYCGRG 3 cut(s) 61, 70, 455
Aor51HI AGCGCT 2 cut(s) 124, 664
AoxI GGCC 3 cut(s) 359, 412, 1243
ApeKI GCWGC 9 cut(s) 46, 125, 128, 156, 171, 229, 1075, 1299, 1413
ApoI RAATTY 4 cut(s) 703, 1538, 1750, 1802
ArsI GACNNNNNNTTYG 2 cut(s) 1426, 1458
AseI ATTAAT 2 cut(s) 1383, 1734
Asp700I GAANNNNTTC 2 cut(s) 1592, 1802
AspLEI GCGC 5 cut(s) 77, 108, 125, 665, 1710
AspS9I GGNCC 3 cut(s) 513, 684, 1244
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 352, 1508, 1828
AvaI CYCGRG 3 cut(s) 61, 70, 455
AvaII GGWCC 2 cut(s) 513, 684
AxyI CCTNAGG 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 661, 694
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 1 cut(s) 1155
BanII GRGCYC 3 cut(s) 94, 1458, 1849
BbsI GAAGAC 1 cut(s) 1733
Bbv12I GWGCWC 2 cut(s) 94, 1849
BbvCI CCTCAGC 1 cut(s) 1151
BbvI GCAGC 9 cut(s) 58, 112, 115, 168, 183, 241, 1087, 1286, 1400
BccI CCATC 7 cut(s) 29, 272, 518, 550, 584, 616, 1474
BceAI ACGGC 2 cut(s) 202, 1143
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 3 cut(s) 521, 1477, 1914
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 4 cut(s) 46, 935, 965, 1427
BfaI CTAG 3 cut(s) 510, 1038, 1224
BfmI CTRYAG 2 cut(s) 227, 1300
BfoI RGCGCY 3 cut(s) 126, 666, 1711
BglII AGATCT 2 cut(s) 245, 913
Bme1390I CCNGG 4 cut(s) 266, 521, 1477, 1914
Bme18I GGWCC 2 cut(s) 513, 684
BmeT110I CYCGRG 3 cut(s) 61, 70, 455
BmgT120I GGNCC 3 cut(s) 513, 684, 1244
BmiI GGNNCC 6 cut(s) 263, 270, 350, 529, 686, 1157
BmrFI CCNGG 4 cut(s) 266, 521, 1477, 1914
BmrI ACTGGG 1 cut(s) 898
BmuI ACTGGG 1 cut(s) 898
BoxI GACNNNNGTC 1 cut(s) 857
BpiI GAAGAC 1 cut(s) 1733
BpmI CTGGAG 1 cut(s) 1904
Bpu10I CCTNAGC 1 cut(s) 1151
BpuEI CTTGAG 1 cut(s) 1550
BpuMI CCSGG 1 cut(s) 266
BsaAI YACGTR 1 cut(s) 1340
BsaBI GATNNNNATC 1 cut(s) 1465
BsaHI GRCGYC 2 cut(s) 39, 626
BsaJI CCNNGG 2 cut(s) 1427, 1812
Bsc4I CCNNNNNNNGG 6 cut(s) 271, 278, 1146, 1369, 1476, 1913
Bse1I ACTGG 5 cut(s) 484, 893, 1370, 1470, 1887
Bse21I CCTNAGG 1 cut(s) 353
Bse3DI GCAATG 2 cut(s) 84, 199
Bse8I GATNNNNATC 1 cut(s) 1465
BseBI CCWGG 3 cut(s) 521, 1477, 1914
BseDI CCNNGG 2 cut(s) 1427, 1812
BseGI GGATG 8 cut(s) 426, 529, 561, 958, 1333, 1466, 1540, 1752
BseJI GATNNNNATC 1 cut(s) 1465
BseLI CCNNNNNNNGG 6 cut(s) 271, 278, 1146, 1369, 1476, 1913
BseMI GCAATG 2 cut(s) 84, 199
BseMII CTCAG 3 cut(s) 344, 1142, 1462
BseNI ACTGG 5 cut(s) 484, 893, 1370, 1470, 1887
BseRI GAGGAG 2 cut(s) 370, 1337
BseXI GCAGC 9 cut(s) 58, 112, 115, 168, 183, 241, 1087, 1286, 1400
Bsh1236I CGCG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 3 cut(s) 361, 414, 1245
BshNI GGYRCC 1 cut(s) 1155
BsiEI CGRYCG 1 cut(s) 692
BsiHKAI GWGCWC 2 cut(s) 94, 1849
BsiHKCI CYCGRG 3 cut(s) 61, 70, 455
BsiSI CCGG 2 cut(s) 266, 272
BslFI GGGAC 2 cut(s) 670, 1402
BslI CCNNNNNNNGG 6 cut(s) 271, 278, 1146, 1369, 1476, 1913
BsmAI GTCTC 4 cut(s) 46, 935, 965, 1427
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 670, 1402
BsmI GAATGC 2 cut(s) 1233, 1350
BsnI GGCC 3 cut(s) 361, 414, 1245
BsoBI CYCGRG 3 cut(s) 61, 70, 455
Bsp1286I GDGCHC 3 cut(s) 94, 1458, 1849
Bsp143I GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
BspACI CCGC 6 cut(s) 132, 189, 198, 338, 789, 1231
BspANI GGCC 3 cut(s) 361, 414, 1245
BspCNI CTCAG 3 cut(s) 345, 1143, 1461
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 6 cut(s) 263, 270, 350, 529, 686, 1157
BspMAI CTGCAG 2 cut(s) 231, 1304
BspPI GGATC 4 cut(s) 376, 499, 567, 821
BspQI GCTCTTC 2 cut(s) 1263, 1575
BspT107I GGYRCC 1 cut(s) 1155
BsrDI GCAATG 2 cut(s) 84, 199
BsrI ACTGG 5 cut(s) 484, 893, 1370, 1470, 1887
BssECI CCNNGG 2 cut(s) 1427, 1812
BssMI GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
BssNI GRCGYC 2 cut(s) 39, 626
BssT1I CCWWGG 2 cut(s) 1427, 1812
Bst2UI CCWGG 3 cut(s) 521, 1477, 1914
Bst4CI ACNGT 4 cut(s) 641, 698, 764, 1068
Bst6I CTCTTC 4 cut(s) 395, 648, 1263, 1575
BstACI GRCGYC 2 cut(s) 39, 626
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 4 cut(s) 31, 121, 1287, 1867
BstDEI CTNAG 5 cut(s) 353, 837, 1032, 1151, 1448
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 8 cut(s) 426, 529, 561, 958, 1333, 1466, 1540, 1752
BstFNI CGCG 1 cut(s) 431
BstH2I RGCGCY 3 cut(s) 126, 666, 1711
BstHHI GCGC 5 cut(s) 77, 108, 125, 665, 1710
BstKTI GATC 8 cut(s) 226, 248, 371, 494, 562, 816, 877, 916
BstMAI GTCTC 4 cut(s) 46, 935, 965, 1427
BstMBI GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 6 cut(s) 125, 131, 195, 238, 1453, 1499
BstNI CCWGG 3 cut(s) 521, 1477, 1914
BstNSI RCATGY 1 cut(s) 1615
BstPAI GACNNNNGTC 1 cut(s) 857
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 4 cut(s) 264, 519, 1475, 1912
BstSFI CTRYAG 2 cut(s) 227, 1300
BstSNI TACGTA 1 cut(s) 1340
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 9 cut(s) 58, 112, 115, 168, 183, 241, 1087, 1286, 1400
BstV2I GAAGAC 1 cut(s) 1733
BstX2I RGATCY 5 cut(s) 245, 368, 491, 813, 913
BstYI RGATCY 5 cut(s) 245, 368, 491, 813, 913
Bsu36I CCTNAGG 1 cut(s) 353
BsuRI GGCC 3 cut(s) 361, 414, 1245
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 8 cut(s) 426, 529, 561, 958, 1333, 1466, 1540, 1752
BtsI GCAGTG 1 cut(s) 1698
BtsIMutI CAGTG 6 cut(s) 703, 719, 1016, 1064, 1363, 1698
Cac8I GCNNGC 4 cut(s) 31, 121, 1287, 1867
CaiI CAGNNNCTG 1 cut(s) 1365
CfoI GCGC 5 cut(s) 77, 108, 125, 665, 1710
Cfr13I GGNCC 3 cut(s) 513, 684, 1244
CseI GACGC 4 cut(s) 28, 615, 758, 862
Csp6I GTAC 2 cut(s) 214, 1473
CviAII CATG 5 cut(s) 320, 538, 602, 1612, 1681
CviQI GTAC 2 cut(s) 214, 1473
DdeI CTNAG 5 cut(s) 353, 837, 1032, 1151, 1448
DpnI GATC 8 cut(s) 225, 247, 370, 493, 561, 815, 876, 915
DpnII GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
DraI TTTAAA 2 cut(s) 549, 910
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 4 cut(s) 395, 648, 1263, 1575
EarI CTCTTC 4 cut(s) 395, 648, 1263, 1575
Ecl136II GAGCTC 2 cut(s) 92, 1847
Eco105I TACGTA 1 cut(s) 1340
Eco130I CCWWGG 2 cut(s) 1427, 1812
Eco24I GRGCYC 3 cut(s) 94, 1458, 1849
Eco32I GATATC 2 cut(s) 958, 1023
Eco47I GGWCC 2 cut(s) 513, 684
Eco47III AGCGCT 2 cut(s) 124, 664
Eco53kI GAGCTC 2 cut(s) 92, 1847
Eco57I CTGAAG 1 cut(s) 485
Eco81I CCTNAGG 1 cut(s) 353
Eco88I CYCGRG 3 cut(s) 61, 70, 455
Eco91I GGTNACC 1 cut(s) 5
EcoICRI GAGCTC 2 cut(s) 92, 1847
EcoO109I RGGNCCY 1 cut(s) 1244
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 1538
EcoRII CCWGG 3 cut(s) 519, 1475, 1912
EcoRV GATATC 2 cut(s) 958, 1023
EcoT14I CCWWGG 2 cut(s) 1427, 1812
EcoT38I GRGCYC 3 cut(s) 94, 1458, 1849
ErhI CCWWGG 2 cut(s) 1427, 1812
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 5 cut(s) 323, 541, 605, 1615, 1684
FalI AAGNNNNNCTT 2 cut(s) 1420, 1452
FaqI GGGAC 2 cut(s) 670, 1402
FatI CATG 5 cut(s) 319, 537, 601, 1611, 1680
FauI CCCGC 1 cut(s) 191
FauNDI CATATG 1 cut(s) 1669
FbaI TGATCA 1 cut(s) 874
FokI GGATG 8 cut(s) 433, 536, 568, 965, 1340, 1453, 1547, 1759
FriOI GRGCYC 3 cut(s) 94, 1458, 1849
FspBI CTAG 3 cut(s) 510, 1038, 1224
GlaI GCGC 5 cut(s) 76, 107, 124, 664, 1709
GsuI CTGGAG 1 cut(s) 1904
HaeII RGCGCY 3 cut(s) 126, 666, 1711
HaeIII GGCC 3 cut(s) 361, 414, 1245
HapII CCGG 2 cut(s) 266, 272
HgaI GACGC 4 cut(s) 28, 615, 758, 862
HhaI GCGC 5 cut(s) 77, 108, 125, 665, 1710
Hin1I GRCGYC 2 cut(s) 39, 626
Hin1II CATG 5 cut(s) 323, 541, 605, 1615, 1684
Hin6I GCGC 5 cut(s) 75, 106, 123, 663, 1708
HinP1I GCGC 5 cut(s) 75, 106, 123, 663, 1708
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 4 cut(s) 407, 1500, 1823, 1891
HpaII CCGG 2 cut(s) 266, 272
HphI GGTGA 3 cut(s) 352, 1508, 1828
Hpy166II GTNNAC 3 cut(s) 55, 382, 637
Hpy8I GTNNAC 3 cut(s) 55, 382, 637
Hpy99I CGWCG 3 cut(s) 209, 631, 856
HpyAV CCTTC 8 cut(s) 29, 841, 876, 1270, 1363, 1392, 1775, 1929
HpyCH4III ACNGT 4 cut(s) 641, 698, 764, 1068
HpyCH4IV ACGT 1 cut(s) 1339
HpyCH4V TGCA 6 cut(s) 229, 717, 1289, 1302, 1522, 1696
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 131, 195, 238, 1453, 1499
HpyF3I CTNAG 5 cut(s) 353, 837, 1032, 1151, 1448
HpySE526I ACGT 1 cut(s) 1339
Hsp92I GRCGYC 2 cut(s) 39, 626
Hsp92II CATG 5 cut(s) 323, 541, 605, 1615, 1684
HspAI GCGC 5 cut(s) 75, 106, 123, 663, 1708
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
LguI GCTCTTC 2 cut(s) 1263, 1575
LmnI GCTCC 3 cut(s) 1195, 1844, 1852
Lsp1109I GCAGC 9 cut(s) 58, 112, 115, 168, 183, 241, 1087, 1286, 1400
MaeI CTAG 3 cut(s) 510, 1038, 1224
MaeII ACGT 1 cut(s) 1339
MaeIII GTNAC 3 cut(s) 5, 176, 764
MalI GATC 8 cut(s) 225, 247, 370, 493, 561, 815, 876, 915
MboI GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
MfeI CAATTG 2 cut(s) 363, 922
MflI RGATCY 5 cut(s) 245, 368, 491, 813, 913
MhlI GDGCHC 3 cut(s) 94, 1458, 1849
MlsI TGGCCA 1 cut(s) 414
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 5 cut(s) 60, 76, 281, 527, 937
MmeI TCCRAC 3 cut(s) 540, 555, 1822
Mox20I TGGCCA 1 cut(s) 414
MroXI GAANNNNTTC 2 cut(s) 1592, 1802
MscI TGGCCA 1 cut(s) 414
MseI TTAA 5 cut(s) 548, 909, 1383, 1722, 1734
MslI CAYNNNNRTG 2 cut(s) 318, 576
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 2 cut(s) 232, 745
MspI CCGG 2 cut(s) 266, 272
MspR9I CCNGG 4 cut(s) 266, 521, 1477, 1914
MunI CAATTG 2 cut(s) 363, 922
Mva1269I GAATGC 2 cut(s) 1233, 1350
MvaI CCWGG 3 cut(s) 521, 1477, 1914
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 6 cut(s) 125, 131, 195, 238, 1453, 1499
NciI CCSGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 1669
NdeII GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
NlaIII CATG 5 cut(s) 323, 541, 605, 1615, 1684
NlaIV GGNNCC 6 cut(s) 263, 270, 350, 529, 686, 1157
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 5
NspI RCATGY 1 cut(s) 1615
PciI ACATGT 1 cut(s) 1611
PciSI GCTCTTC 2 cut(s) 1263, 1575
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 1345
PctI GAATGC 2 cut(s) 1233, 1350
PdmI GAANNNNTTC 2 cut(s) 1592, 1802
PfeI GAWTC 8 cut(s) 66, 460, 541, 796, 996, 1106, 1588, 1600
PleI GAGTC 5 cut(s) 59, 76, 281, 527, 937
PpsI GAGTC 5 cut(s) 59, 76, 281, 527, 937
Ppu21I YACGTR 1 cut(s) 1340
PscI ACATGT 1 cut(s) 1611
PshAI GACNNNNGTC 1 cut(s) 857
PshBI ATTAAT 2 cut(s) 1383, 1734
PsiI TTATAA 2 cut(s) 776, 1047
Psp124BI GAGCTC 2 cut(s) 94, 1849
Psp6I CCWGG 3 cut(s) 519, 1475, 1912
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 519, 1475, 1912
PspN4I GGNNCC 6 cut(s) 263, 270, 350, 529, 686, 1157
PspPI GGNCC 3 cut(s) 513, 684, 1244
PstI CTGCAG 2 cut(s) 231, 1304
PstNI CAGNNNCTG 1 cut(s) 1365
PsuI RGATCY 5 cut(s) 245, 368, 491, 813, 913
PvuII CAGCTG 2 cut(s) 232, 745
RsaI GTAC 2 cut(s) 215, 1474
RsaNI GTAC 2 cut(s) 214, 1473
RseI CAYNNNNRTG 2 cut(s) 318, 576
SacI GAGCTC 2 cut(s) 94, 1849
SapI GCTCTTC 2 cut(s) 1263, 1575
SaqAI TTAA 5 cut(s) 548, 909, 1383, 1722, 1734
Sau3AI GATC 8 cut(s) 223, 245, 368, 491, 559, 813, 874, 913
Sau96I GGNCC 3 cut(s) 513, 684, 1244
SchI GAGTC 5 cut(s) 60, 76, 281, 527, 937
ScrFI CCNGG 4 cut(s) 266, 521, 1477, 1914
SduI GDGCHC 3 cut(s) 94, 1458, 1849
SfcI CTRYAG 2 cut(s) 227, 1300
SinI GGWCC 2 cut(s) 513, 684
SmiMI CAYNNNNRTG 2 cut(s) 318, 576
SmlI CTYRAG 1 cut(s) 1529
SmoI CTYRAG 1 cut(s) 1529
SnaBI TACGTA 1 cut(s) 1340
SsiI CCGC 6 cut(s) 132, 189, 198, 338, 789, 1231
SspMI CTAG 3 cut(s) 510, 1038, 1224
SstI GAGCTC 2 cut(s) 94, 1849
StyD4I CCNGG 4 cut(s) 264, 519, 1475, 1912
StyI CCWWGG 2 cut(s) 1427, 1812
TaaI ACNGT 4 cut(s) 641, 698, 764, 1068
TaiI ACGT 1 cut(s) 1342
TaqI TCGA 4 cut(s) 999, 1111, 1767, 1833
TaqII GACCGA 1 cut(s) 706
TauI GCSGC 3 cut(s) 134, 192, 792
TfiI GAWTC 8 cut(s) 66, 460, 541, 796, 996, 1106, 1588, 1600
Tru1I TTAA 5 cut(s) 548, 909, 1383, 1722, 1734
Tru9I TTAA 5 cut(s) 548, 909, 1383, 1722, 1734
TscAI CASTG 6 cut(s) 703, 719, 1023, 1071, 1370, 1698
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 9 cut(s) 46, 125, 128, 156, 171, 229, 1075, 1299, 1413
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 7 cut(s) 554, 860, 925, 1250, 1344, 1551, 1763
TspGWI ACGGA 3 cut(s) 568, 1118, 1689
TspRI CASTG 6 cut(s) 703, 719, 1023, 1071, 1370, 1698
VpaK11BI GGWCC 2 cut(s) 513, 684
VspI ATTAAT 2 cut(s) 1383, 1734
XapI RAATTY 4 cut(s) 703, 1538, 1750, 1802
XbaI TCTAGA 1 cut(s) 1223
XceI RCATGY 1 cut(s) 1615
XcmI CCANNNNNNNNNTGG 1 cut(s) 1483
XmnI GAANNNNTTC 2 cut(s) 1592, 1802
XspI CTAG 3 cut(s) 510, 1038, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.