Rroxscaffold_2G00146850

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015446
Physical Location & Seq
Forward (+)
84734378 .. 84738660
4283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146850.1

Sequence Viewer

Length: 1140 bp
ATGCAGATTTGTGATATGGTTGCTGTTGCTAAGATTATGAAGGCAACGCTTGTCCTACCTTCACTTGATCACACCTCCTACTGGGCTGATGAGAGTGGCTTTAAAGATCTGTTTGATTGGCAACACTTCATTGAGACATTGAAGGATGATATCCACATAATTGAGACATTACCACCTGAATATGCAGGAATTGAACCTTTCAACAAAACACCAATATCTTGGTCTAAGGCAAGTTATTATAAGTCAGAGGTTCTCCCACTGCTGAAGCAGCACACGGCAGTCTATCTCACTCATACTGATTCTCGGCTTTCCAACAATGGGCTTCCAAGTTCCATTCAAAAACTGAGGTGTCGTGTGAATTACAGGGCATTGAAATATTCAGCTCCAATAGAAGAACTCGGAAAAACCTTGATTTCTAGAATGAGGCAGAATGGAGGTCCTTACCTTGCTCTCCACTTGAGGTACGAGAAGGATATGCTTGCATTTACGGGCTGCAGTCATAGTTTGACAGCAGAAGAGGATGAAGAACTACGCAGAATGCGCTATGAAGTCAGCCACTGGAAGGAGAAAGAGATTAATGGGACAGAGAGAAGGTTGCTTGGTGGTTGTCCATTGACCCCTAGGGAGACTTCTCTTTTGCTCCGAGGGCTGGGTTTTCCTTCAAGCACCAGGATGTACTTGGTAGCTGGTGAAGCTTATGGGAATGGTAGCATGCGACATCTTGAAGATGACTTCCCGAACATCTTCTCCCATACGACTCTCGCCTCAGAAGAAGAACTGAGTCCATTCAGGGATCATCAGAACATGTTGGCTGGTATAGACTATGCTGTTGCCTTAGAGAGTGATGCTTTTCTTTATACATATGATGGAAACATGGCAAAAGCTGTTCAAGGGCATAGGCGCTTTGAGAACTTTAAGAAGACCATCAATCCAGACAAGATGAATTTTGTGAAACTTGTTGATGATTTTGACCAAGGAAAAATCTCTTGGAAGAAGTTTAGTTCTAAGGTGAAGAAGCTTCACCATGACAGAGATGGGTCTCCATATTTGAGGGAGCCTGGAGAGGTTCCGAAGCTGGAAGAGAGTTTCTATGCAAATCCTTTTCCAGGCTGTATTTGTGAAACAAGATGGAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

379

Amino Acids

43.59

Weight (kDa)

6.71

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 302 3.3e-95 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35570
fragaria_vesca FvH4_1g08590
malus_domestica MD02G1090100.v1.1 MD15G1216700.v1.1
prunus_persica Prupe.7G199900_v2.0.a1
pyrus_communis pycom02g07200
rosa_chinensis RchiOBHm_Chr2g0094811
rosa_laevigata RLG00000016523
rosa_multiflora Rmu_sc0006422.1_g000026 Rmu_sc0011074.1_g000001
rosa_roxburghii Rroxscaffold_2G00146850
rosa_rugosa Rorug02G0047100
rosa_samantha Rh2AG094600 Rh2BG095200 Rh2CG097000 Rh2DG094000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AclWI GGATC 1 cut(s) 801
AcsI RAATTY 1 cut(s) 943
AcuI CTGAAG 1 cut(s) 284
AfaI GTAC 2 cut(s) 464, 677
AfiI CCNNNNNNNGG 5 cut(s) 81, 318, 562, 649, 1106
AflIII ACRYGT 1 cut(s) 804
AgsI TTSAA 8 cut(s) 142, 194, 202, 338, 373, 663, 725, 890
AjnI CCWGG 3 cut(s) 668, 1057, 1105
AjuI GAANNNNNNNTTGG 2 cut(s) 970, 1002
AloI GAACNNNNNNTCC 2 cut(s) 731, 763
AluBI AGCT 6 cut(s) 383, 686, 695, 884, 1018, 1075
AluI AGCT 6 cut(s) 383, 686, 695, 884, 1018, 1075
Alw26I GTCTC 4 cut(s) 128, 158, 620, 1044
AlwI GGATC 1 cut(s) 801
AlwNI CAGNNNCTG 1 cut(s) 558
ApeKI GCWGC 2 cut(s) 268, 492
ApoI RAATTY 1 cut(s) 943
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 743
AspA2I CCTAGG 1 cut(s) 620
AspLEI GCGC 2 cut(s) 543, 903
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 701, 1013, 1021
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 620
BbsI GAAGAC 1 cut(s) 926
BbvI GCAGC 2 cut(s) 280, 479
BccI CCATC 4 cut(s) 860, 932, 1028, 1122
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 3 cut(s) 670, 1059, 1107
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 4 cut(s) 128, 158, 620, 1044
BfaI CTAG 2 cut(s) 417, 621
BfmI CTRYAG 1 cut(s) 493
BfoI RGCGCY 1 cut(s) 904
BglII AGATCT 1 cut(s) 106
BisI GCNGC 2 cut(s) 269, 493
BlnI CCTAGG 1 cut(s) 620
BlsI GCNGC 2 cut(s) 270, 494
Bme1390I CCNGG 3 cut(s) 670, 1059, 1107
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 2 cut(s) 1056, 1068
BmrFI CCNGG 3 cut(s) 670, 1059, 1107
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 835
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 926
BpmI CTGGAG 1 cut(s) 1080
BpuEI CTTGAG 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 3 cut(s) 620, 643, 973
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 318, 562, 649, 1106
Bse1I ACTGG 2 cut(s) 86, 563
BseBI CCWGG 3 cut(s) 670, 1059, 1107
BseDI CCNNGG 3 cut(s) 620, 643, 973
BseGI GGATG 3 cut(s) 151, 526, 678
BseLI CCNNNNNNNGG 5 cut(s) 81, 318, 562, 649, 1106
BseMII CTCAG 3 cut(s) 335, 770, 780
BseNI ACTGG 2 cut(s) 86, 563
BseXI GCAGC 2 cut(s) 280, 479
BseYI CCCAGC 1 cut(s) 649
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 5 cut(s) 81, 318, 562, 649, 1106
BsmAI GTCTC 4 cut(s) 128, 158, 620, 1044
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 543
Bso31I GGTCTC 1 cut(s) 1044
Bsp143I GATC 3 cut(s) 67, 106, 793
BspCNI CTCAG 3 cut(s) 336, 771, 779
BspLI GGNNCC 2 cut(s) 1056, 1068
BspMAI CTGCAG 1 cut(s) 497
BspPI GGATC 1 cut(s) 801
BspTNI GGTCTC 1 cut(s) 1044
BsrI ACTGG 2 cut(s) 86, 563
BssECI CCNNGG 3 cut(s) 620, 643, 973
BssMI GATC 3 cut(s) 67, 106, 793
BssT1I CCWWGG 2 cut(s) 620, 973
Bst2UI CCWGG 3 cut(s) 670, 1059, 1107
Bst6I CTCTTC 2 cut(s) 510, 1074
BstC8I GCNNGC 2 cut(s) 480, 713
BstDEI CTNAG 7 cut(s) 30, 225, 344, 766, 779, 835, 1005
BstENI CCTNNNNNAGG 1 cut(s) 1104
BstF5I GGATG 3 cut(s) 151, 526, 678
BstH2I RGCGCY 1 cut(s) 904
BstHHI GCGC 2 cut(s) 543, 903
BstKTI GATC 3 cut(s) 70, 109, 796
BstMAI GTCTC 4 cut(s) 128, 158, 620, 1044
BstMBI GATC 3 cut(s) 67, 106, 793
BstMWI GCNNNNNNNGC 4 cut(s) 268, 540, 646, 692
BstNI CCWGG 3 cut(s) 670, 1059, 1107
BstNSI RCATGY 2 cut(s) 715, 808
BstSCI CCNGG 3 cut(s) 668, 1057, 1105
BstSFI CTRYAG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 280, 479
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 1 cut(s) 106
BstXI CCANNNNNNTGG 1 cut(s) 219
BstYI RGATCY 1 cut(s) 106
BtsCI GGATG 3 cut(s) 151, 526, 678
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 556
Cac8I GCNNGC 2 cut(s) 480, 713
CaiI CAGNNNCTG 1 cut(s) 558
CfoI GCGC 2 cut(s) 543, 903
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 463, 676
CviAII CATG 4 cut(s) 712, 805, 874, 1025
CviQI GTAC 2 cut(s) 463, 676
DdeI CTNAG 7 cut(s) 30, 225, 344, 766, 779, 835, 1005
DpnI GATC 3 cut(s) 69, 108, 795
DpnII GATC 3 cut(s) 67, 106, 793
DraI TTTAAA 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 510, 1074
EarI CTCTTC 2 cut(s) 510, 1074
Eco130I CCWWGG 2 cut(s) 620, 973
Eco31I GGTCTC 1 cut(s) 1044
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 1104
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 3 cut(s) 668, 1057, 1105
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 620, 973
ErhI CCWWGG 2 cut(s) 620, 973
FaeI CATG 4 cut(s) 715, 808, 877, 1028
FaqI GGGAC 1 cut(s) 595
FatI CATG 4 cut(s) 711, 804, 873, 1024
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 269, 493
FokI GGATG 3 cut(s) 158, 533, 685
Fsp4HI GCNGC 2 cut(s) 269, 493
FspBI CTAG 2 cut(s) 417, 621
GlaI GCGC 2 cut(s) 542, 902
GluI GCNGC 2 cut(s) 269, 493
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 1080
HaeII RGCGCY 1 cut(s) 904
HhaI GCGC 2 cut(s) 543, 903
Hin1II CATG 4 cut(s) 715, 808, 877, 1028
Hin6I GCGC 2 cut(s) 541, 901
HinP1I GCGC 2 cut(s) 541, 901
HindIII AAGCTT 2 cut(s) 693, 1016
HinfI GANTC 3 cut(s) 299, 757, 781
HphI GGTGA 3 cut(s) 701, 1013, 1021
Hpy188I TCNGA 6 cut(s) 247, 401, 644, 769, 801, 1071
Hpy188III TCNNGA 4 cut(s) 417, 722, 736, 932
HpyAV CCTTC 7 cut(s) 34, 69, 136, 463, 556, 585, 669
HpyCH4V TGCA 5 cut(s) 4, 185, 482, 495, 1094
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 540, 646, 692
HpyF3I CTNAG 7 cut(s) 30, 225, 344, 766, 779, 835, 1005
Hsp92II CATG 4 cut(s) 715, 808, 877, 1028
HspAI GCGC 2 cut(s) 541, 901
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 3 cut(s) 67, 106, 793
LmnI GCTCC 3 cut(s) 388, 645, 1054
Lsp1109I GCAGC 2 cut(s) 280, 479
LweI GCATC 1 cut(s) 835
MaeI CTAG 2 cut(s) 417, 621
MalI GATC 3 cut(s) 69, 108, 795
MboI GATC 3 cut(s) 67, 106, 793
MflI RGATCY 1 cut(s) 106
MluCI AATT 4 cut(s) 159, 189, 358, 943
MlyI GAGTC 2 cut(s) 751, 790
MmeI TCCRAC 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 743
MseI TTAA 3 cut(s) 102, 576, 915
MslI CAYNNNNRTG 1 cut(s) 671
MspR9I CCNGG 3 cut(s) 670, 1059, 1107
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 3 cut(s) 670, 1059, 1107
MwoI GCNNNNNNNGC 4 cut(s) 268, 540, 646, 692
NdeI CATATG 1 cut(s) 862
NdeII GATC 3 cut(s) 67, 106, 793
NlaIII CATG 4 cut(s) 715, 808, 877, 1028
NlaIV GGNNCC 2 cut(s) 1056, 1068
NmeAIII GCCGAG 1 cut(s) 283
NspI RCATGY 2 cut(s) 715, 808
PaeI GCATGC 1 cut(s) 715
PciI ACATGT 1 cut(s) 804
PctI GAATGC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 2 cut(s) 270, 494
PleI GAGTC 2 cut(s) 751, 789
PpsI GAGTC 2 cut(s) 751, 789
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 804
PshBI ATTAAT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 3 cut(s) 668, 1057, 1105
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 3 cut(s) 668, 1057, 1105
PspN4I GGNNCC 2 cut(s) 1056, 1068
PspPI GGNCC 1 cut(s) 437
PspPPI RGGWCCY 1 cut(s) 437
PstI CTGCAG 1 cut(s) 497
PstNI CAGNNNCTG 1 cut(s) 558
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 2 cut(s) 464, 677
RsaNI GTAC 2 cut(s) 463, 676
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 3 cut(s) 102, 576, 915
SatI GCNGC 2 cut(s) 269, 493
Sau3AI GATC 3 cut(s) 67, 106, 793
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 2 cut(s) 751, 790
ScrFI CCNGG 3 cut(s) 670, 1059, 1107
SfaNI GCATC 1 cut(s) 835
SfcI CTRYAG 1 cut(s) 493
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
SphI GCATGC 1 cut(s) 715
Sse9I AATT 4 cut(s) 159, 189, 358, 943
SspI AATATT 1 cut(s) 377
SspMI CTAG 2 cut(s) 417, 621
StyD4I CCNGG 3 cut(s) 668, 1057, 1105
StyI CCWWGG 2 cut(s) 620, 973
TasI AATT 4 cut(s) 159, 189, 358, 943
TatI WGTACW 1 cut(s) 675
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 3 cut(s) 102, 576, 915
Tru9I TTAA 3 cut(s) 102, 576, 915
TscAI CASTG 2 cut(s) 264, 563
TseI GCWGC 2 cut(s) 268, 492
TspDTI ATGAA 5 cut(s) 53, 118, 537, 561, 956
TspRI CASTG 2 cut(s) 264, 563
VpaK11BI GGWCC 1 cut(s) 437
VspI ATTAAT 1 cut(s) 576
XagI CCTNNNNNAGG 1 cut(s) 1104
XapI RAATTY 1 cut(s) 943
XbaI TCTAGA 1 cut(s) 416
XceI RCATGY 2 cut(s) 715, 808
XcmI CCANNNNNNNNNTGG 2 cut(s) 676, 1031
XmaJI CCTAGG 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 2 cut(s) 417, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.