RLG00000016523

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015446
Physical Location & Seq
Reverse (-)
7313329 .. 7316889
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016523

Sequence Viewer

Length: 1914 bp
ATGGGCCACCACCACCACCTCCACAACACCACCGCCGACGGCGGCGTCTCCCAGCGAGTCAACAGCCCCAGGTTCTCCGGCGCCATGACCCGCCGCGCGCACTCCTTCAAGCGCAACCCTTTCTCCTCCTCCGCCGGCGCCGGCCACAATGACGACGGCGGCGGCGGCGGGTTCAGTACCCAGTACGAGGTGGACCTGCAGATGACCTCGCCGAGAGCGGAGATCGGCGGGAGCTCCGGCTCCGGCGAGGGGTTTGGTGAGTCGGCGGCGGAGAGGAAGCAGCAGAGCAGCCACGTGACGCAGAGAGCGGCGGTGAGAGGGTTTCTGAGGAAGCCGATTGAGGCGGTTATGGTGGAGATGGGTTTGAAGGAAAGGAAGAGACTTGGGCATTGGATGTTCTTTGCGTTTTGTGGGCTTTGTTTGTTTCTGGGGATTCTCAAGATTTGTGCTACTGGCTGGTTTGGATCTGCTATTGAAACTGCAAGGTCCAATCAGGATAATTCTGGCTCCACGGCTCATTCAAATCGGATTGATCAAAGCTCTCATGATTATGGGTATAGAGAGGGAGGAGGTGATGTTGAACGGACTCTAATGATGGTATCGTCTGGTGTGGTTGGTAGAGAGAACAGAGCTGAATACACAGGTATCTGGTCGCAACCCAACAGTGCGAATTACACTCAGTGCATTGACCATCCAAAAAGCCACAAAAAGCTAGATCCAAAGACCAATGGTTACATTCTTATAAATGCCAATGGTGGCTTGAATCAGATGAGATTTGGGATTTGTGATATGGTTGCTGTTGCTAAGATTATGAAGGCAACGCTTGTCCTACCATCACTTGATCACACCTCCTACTGGGCTGATGAGAGTGGCTTTAAAGATCTGTTTGATTGGCAACACTTCATTGAGACATTGAAGGATGATATCCACATAGTTGAGACATTACCACCTGAATATGCAGGAATTGAACCTTTCAACAAAACACCAATATCTTGGTCCAAGGCAAGTTATTATAAGTCAGAGGTTCTCCCACTGCTGAAGCAGCACACGGCAGTCTATCTCACTCATACTGATTCTCGGCTTTCCAACAATGGGCTCCCAAGTTCCATTCAAAAACTGAGGTGTCGTGTGAATTACAGGGCATTAAAATATTCAGCTCCAATAGAAGAACTCGGAAAAACCTTGGTTTGTAGAATGAGGCAGAATGGAGGTCCTTACCTTGCTCTCCACTTGAGGTATGAGAAGGATATGCTTGCATTTACGGGCTGCAGTCATAGTTTGACAGCAGAAGAGGATGAAGAACTACGCAGAATGCGCTATGAAGTCAGCCACTGGAAGGAGAAAGAGATTAATGGGACAGAGAGAAGGTTGCTTGGTGGTTGTCCATTGACCCCTAGGGAGACTTCTCTTTTGCTCCGAGGGCTGGGTTTTCCTTCAAGCACCAGGATTTACTTGGTAGCTGGTGAAGCTTATGGGAATGGTAGCATGCGACATCTTGAGGATGACTTCCCGAACATCTTCTCCCATTCGACTCTCGCCTCAGAAGAAGAACTGAGTCCATTCAGGGATCATCAGAACATGTTGGCTGGTATAGATTATGCTGTTGCCTTAGAGAGTGATGCTTTTCTTTATACATATGATGGAAACATGGCAAAGGCTGTTCAAGGTCATAGGCGCTTTGAGAACTTTAAGAAGACCATCAATCCAGACAAGATGAATTTTGTGAAACTTGTTGATGATTTTGACCAAGGAAAAATCTCTTGGAAGAAGTTTAGTTCTAAGGTGAAGAAGCTTCACCATGACAGAGATGGGTCTCCATATTTGAGGGAGCCTGGAGAGTTTCCGAAGCTGGAAGAGAGTTTCTATGCAAATCCTTTTCCAGGCTGTATTTGTGAAACAAGATGGAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

638

Amino Acids

71.54

Weight (kDa)

8.32

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 245 - 560 2.5e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35570
fragaria_vesca FvH4_1g08590
malus_domestica MD02G1090100.v1.1 MD15G1216700.v1.1
prunus_persica Prupe.7G199900_v2.0.a1
pyrus_communis pycom02g07200
rosa_chinensis RchiOBHm_Chr2g0094811
rosa_laevigata RLG00000016523
rosa_multiflora Rmu_sc0006422.1_g000026 Rmu_sc0011074.1_g000001
rosa_roxburghii Rroxscaffold_2G00146850
rosa_rugosa Rorug02G0047100
rosa_samantha Rh2AG094600 Rh2BG095200 Rh2CG097000 Rh2DG094000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 743, 1014
AasI GACNNNNNNGTC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 204
AccB1I GGYRCC 2 cut(s) 80, 137
AccBSI CCGCTC 2 cut(s) 218, 308
AccII CGCG 2 cut(s) 96, 98
AclWI GGATC 3 cut(s) 472, 710, 1575
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 1717
AcuI CTGAAG 1 cut(s) 1058
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 3 cut(s) 45, 81, 138
AdeI CACNNNGTG 1 cut(s) 681
AfaI GTAC 2 cut(s) 178, 185
AfiI CCNNNNNNNGG 7 cut(s) 187, 249, 855, 1092, 1336, 1423, 1880
AflIII ACRYGT 1 cut(s) 1578
AjnI CCWGG 4 cut(s) 68, 1442, 1831, 1879
AjuI GAANNNNNNNTTGG 2 cut(s) 1744, 1776
AloI GAACNNNNNNTCC 2 cut(s) 1505, 1537
AluBI AGCT 9 cut(s) 234, 540, 632, 712, 1157, 1460, 1469, 1792, 1849
AluI AGCT 9 cut(s) 234, 540, 632, 712, 1157, 1460, 1469, 1792, 1849
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 6 cut(s) 52, 373, 902, 932, 1394, 1818
AlwI GGATC 3 cut(s) 472, 710, 1575
AlwNI CAGNNNCTG 1 cut(s) 1332
AoxI GGCC 2 cut(s) 4, 142
ApeKI GCWGC 4 cut(s) 280, 288, 1042, 1266
ApoI RAATTY 1 cut(s) 1717
ArsI GACNNNNNNTTYG 2 cut(s) 1393, 1425
AseI ATTAAT 1 cut(s) 1350
Asp700I GAANNNNTTC 1 cut(s) 1517
AspA2I CCTAGG 1 cut(s) 1394
AspLEI GCGC 7 cut(s) 83, 98, 100, 114, 140, 1317, 1677
AspS9I GGNCC 5 cut(s) 4, 193, 486, 996, 1211
AsuHPI GGTGA 6 cut(s) 269, 325, 584, 1475, 1787, 1795
AvaII GGWCC 4 cut(s) 193, 486, 996, 1211
AvrII CCTAGG 1 cut(s) 1394
BaeI ACNNNNGTAYC 2 cut(s) 628, 661
BanI GGYRCC 2 cut(s) 80, 137
BanII GRGCYC 2 cut(s) 236, 1098
BbrPI CACGTG 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 1700
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 4 cut(s) 292, 300, 1054, 1253
BccI CCATC 8 cut(s) 352, 589, 699, 841, 1634, 1706, 1802, 1896
BceAI ACGGC 4 cut(s) 55, 172, 528, 1065
BciT130I CCWGG 4 cut(s) 70, 1444, 1833, 1881
BclI TGATCA 2 cut(s) 532, 841
BcoDI GTCTC 6 cut(s) 52, 373, 902, 932, 1394, 1818
BfaI CTAG 2 cut(s) 713, 1395
BfmI CTRYAG 2 cut(s) 197, 1267
BfoI RGCGCY 3 cut(s) 84, 141, 1678
BfuAI ACCTGC 1 cut(s) 204
BglII AGATCT 1 cut(s) 880
BlnI CCTAGG 1 cut(s) 1394
Bme1390I CCNGG 4 cut(s) 70, 1444, 1833, 1881
Bme18I GGWCC 4 cut(s) 193, 486, 996, 1211
BmgT120I GGNCC 5 cut(s) 4, 193, 486, 996, 1211
BmiI GGNNCC 6 cut(s) 82, 139, 241, 508, 1097, 1830
BmrFI CCNGG 4 cut(s) 70, 1444, 1833, 1881
BmrI ACTGGG 2 cut(s) 175, 865
BmsI GCATC 1 cut(s) 1609
BmuI ACTGGG 2 cut(s) 175, 865
BpiI GAAGAC 1 cut(s) 1700
BpmI CTGGAG 1 cut(s) 1854
BpuEI CTTGAG 3 cut(s) 422, 1252, 1517
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 3 cut(s) 45, 81, 138
BsaI GGTCTC 1 cut(s) 1818
BsaJI CCNNGG 7 cut(s) 68, 510, 999, 1182, 1394, 1417, 1747
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 561, 591, 1505, 1535
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 249, 855, 1092, 1336, 1423, 1880
Bse118I RCCGGY 2 cut(s) 134, 140
Bse1I ACTGG 4 cut(s) 181, 457, 860, 1337
BseBI CCWGG 4 cut(s) 70, 1444, 1833, 1881
BseDI CCNNGG 7 cut(s) 68, 510, 999, 1182, 1394, 1417, 1747
BseGI GGATG 5 cut(s) 399, 691, 925, 1300, 1507
BseLI CCNNNNNNNGG 7 cut(s) 187, 249, 855, 1092, 1336, 1423, 1880
BseMII CTCAG 5 cut(s) 317, 692, 1109, 1544, 1554
BseNI ACTGG 4 cut(s) 181, 457, 860, 1337
BsePI GCGCGC 1 cut(s) 96
BseRI GAGGAG 3 cut(s) 115, 118, 582
BseXI GCAGC 4 cut(s) 292, 300, 1054, 1253
BseYI CCCAGC 2 cut(s) 51, 1423
Bsh1236I CGCG 2 cut(s) 96, 98
BshFI GGCC 2 cut(s) 6, 144
BshNI GGYRCC 2 cut(s) 80, 137
BsiHKAI GWGCWC 1 cut(s) 236
BsiSI CCGG 5 cut(s) 78, 135, 141, 237, 243
BslFI GGGAC 1 cut(s) 1369
BslI CCNNNNNNNGG 7 cut(s) 187, 249, 855, 1092, 1336, 1423, 1880
BsmAI GTCTC 6 cut(s) 52, 373, 902, 932, 1394, 1818
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 1369
BsmI GAATGC 1 cut(s) 1317
BsnI GGCC 2 cut(s) 6, 144
Bso31I GGTCTC 1 cut(s) 1818
Bsp1286I GDGCHC 2 cut(s) 236, 1098
Bsp143I GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
BspANI GGCC 2 cut(s) 6, 144
BspCNI CTCAG 5 cut(s) 318, 691, 1110, 1545, 1553
BspFNI CGCG 2 cut(s) 96, 98
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 6 cut(s) 82, 139, 241, 508, 1097, 1830
BspMAI CTGCAG 2 cut(s) 201, 1271
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 3 cut(s) 472, 710, 1575
BspT107I GGYRCC 2 cut(s) 80, 137
BspTNI GGTCTC 1 cut(s) 1818
BsrBI CCGCTC 2 cut(s) 218, 308
BsrFI RCCGGY 2 cut(s) 134, 140
BsrI ACTGG 4 cut(s) 181, 457, 860, 1337
BssAI RCCGGY 2 cut(s) 134, 140
BssECI CCNNGG 7 cut(s) 68, 510, 999, 1182, 1394, 1417, 1747
BssHII GCGCGC 1 cut(s) 96
BssMI GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
BssNI GRCGYC 3 cut(s) 45, 81, 138
BssT1I CCWWGG 4 cut(s) 999, 1182, 1394, 1747
Bst2UI CCWGG 4 cut(s) 70, 1444, 1833, 1881
Bst4CI ACNGT 1 cut(s) 665
Bst6I CTCTTC 3 cut(s) 371, 1284, 1848
BstACI GRCGYC 3 cut(s) 45, 81, 138
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 5 cut(s) 98, 136, 142, 1254, 1487
BstDEI CTNAG 8 cut(s) 326, 678, 804, 1118, 1540, 1553, 1609, 1779
BstDSI CCRYGG 1 cut(s) 510
BstENI CCTNNNNNAGG 1 cut(s) 1878
BstF5I GGATG 5 cut(s) 399, 691, 925, 1300, 1507
BstFNI CGCG 2 cut(s) 96, 98
BstH2I RGCGCY 3 cut(s) 84, 141, 1678
BstHHI GCGC 7 cut(s) 83, 98, 100, 114, 140, 1317, 1677
BstKTI GATC 7 cut(s) 225, 467, 535, 718, 844, 883, 1570
BstMAI GTCTC 6 cut(s) 52, 373, 902, 932, 1394, 1818
BstMBI GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
BstMWI GCNNNNNNNGC 5 cut(s) 165, 1042, 1314, 1420, 1466
BstNI CCWGG 4 cut(s) 70, 1444, 1833, 1881
BstNSI RCATGY 2 cut(s) 1489, 1582
BstSCI CCNGG 4 cut(s) 68, 1442, 1831, 1879
BstSFI CTRYAG 2 cut(s) 197, 1267
BstUI CGCG 2 cut(s) 96, 98
BstV1I GCAGC 4 cut(s) 292, 300, 1054, 1253
BstV2I GAAGAC 1 cut(s) 1700
BstX2I RGATCY 3 cut(s) 464, 715, 880
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 3 cut(s) 464, 715, 880
BsuRI GGCC 2 cut(s) 6, 144
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 5 cut(s) 399, 691, 925, 1300, 1507
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 4 cut(s) 670, 686, 1031, 1330
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 5 cut(s) 98, 136, 142, 1254, 1487
CaiI CAGNNNCTG 1 cut(s) 1332
CciI TCATGA 1 cut(s) 544
CfoI GCGC 7 cut(s) 83, 98, 100, 114, 140, 1317, 1677
Cfr10I RCCGGY 2 cut(s) 134, 140
Cfr13I GGNCC 5 cut(s) 4, 193, 486, 996, 1211
CseI GACGC 2 cut(s) 34, 307
Csp6I GTAC 2 cut(s) 177, 184
CviAII CATG 6 cut(s) 85, 545, 1486, 1579, 1648, 1799
CviQI GTAC 2 cut(s) 177, 184
DdeI CTNAG 8 cut(s) 326, 678, 804, 1118, 1540, 1553, 1609, 1779
DinI GGCGCC 2 cut(s) 82, 139
DpnI GATC 7 cut(s) 224, 466, 534, 717, 843, 882, 1569
DpnII GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
DraI TTTAAA 1 cut(s) 877
DraIII CACNNNGTG 1 cut(s) 681
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 371, 1284, 1848
EarI CTCTTC 3 cut(s) 371, 1284, 1848
EciI GGCGGA 2 cut(s) 121, 284
Ecl136II GAGCTC 1 cut(s) 234
Eco130I CCWWGG 4 cut(s) 999, 1182, 1394, 1747
Eco24I GRGCYC 2 cut(s) 236, 1098
Eco31I GGTCTC 1 cut(s) 1818
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 4 cut(s) 193, 486, 996, 1211
Eco53kI GAGCTC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 1058
Eco72I CACGTG 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 234
EcoNI CCTNNNNNAGG 1 cut(s) 1878
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRII CCWGG 4 cut(s) 68, 1442, 1831, 1879
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 4 cut(s) 999, 1182, 1394, 1747
EcoT38I GRGCYC 2 cut(s) 236, 1098
EgeI GGCGCC 2 cut(s) 82, 139
EheI GGCGCC 2 cut(s) 82, 139
ErhI CCWWGG 4 cut(s) 999, 1182, 1394, 1747
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 6 cut(s) 88, 548, 1489, 1582, 1651, 1802
FaqI GGGAC 1 cut(s) 1369
FatI CATG 6 cut(s) 84, 544, 1485, 1578, 1647, 1798
FauI CCCGC 3 cut(s) 98, 161, 221
FauNDI CATATG 1 cut(s) 1636
FbaI TGATCA 2 cut(s) 532, 841
FokI GGATG 5 cut(s) 406, 678, 932, 1307, 1514
FriOI GRGCYC 2 cut(s) 236, 1098
FspBI CTAG 2 cut(s) 713, 1395
GlaI GCGC 7 cut(s) 82, 97, 99, 113, 139, 1316, 1676
GsaI CCCAGC 2 cut(s) 55, 1427
GsuI CTGGAG 1 cut(s) 1854
HaeII RGCGCY 3 cut(s) 84, 141, 1678
HaeIII GGCC 2 cut(s) 6, 144
HapII CCGG 5 cut(s) 78, 135, 141, 237, 243
HgaI GACGC 2 cut(s) 34, 307
HhaI GCGC 7 cut(s) 83, 98, 100, 114, 140, 1317, 1677
Hin1I GRCGYC 3 cut(s) 45, 81, 138
Hin1II CATG 6 cut(s) 88, 548, 1489, 1582, 1651, 1802
Hin6I GCGC 7 cut(s) 81, 96, 98, 112, 138, 1315, 1675
HinP1I GCGC 7 cut(s) 81, 96, 98, 112, 138, 1315, 1675
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 2 cut(s) 1467, 1790
HinfI GANTC 8 cut(s) 57, 260, 433, 586, 763, 1073, 1531, 1555
HpaII CCGG 5 cut(s) 78, 135, 141, 237, 243
HphI GGTGA 6 cut(s) 269, 325, 584, 1475, 1787, 1795
Hpy166II GTNNAC 2 cut(s) 61, 193
Hpy188I TCNGA 9 cut(s) 327, 528, 768, 1021, 1175, 1418, 1543, 1575, 1845
Hpy188III TCNNGA 6 cut(s) 439, 494, 545, 1496, 1510, 1706
Hpy8I GTNNAC 2 cut(s) 61, 193
Hpy99I CGWCG 2 cut(s) 41, 158
HpyAV CCTTC 8 cut(s) 115, 361, 808, 910, 1237, 1330, 1359, 1443
HpyCH4III ACNGT 1 cut(s) 665
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 7 cut(s) 199, 482, 684, 959, 1256, 1269, 1868
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 1042, 1314, 1420, 1466
HpyF3I CTNAG 8 cut(s) 326, 678, 804, 1118, 1540, 1553, 1609, 1779
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 3 cut(s) 45, 81, 138
Hsp92II CATG 6 cut(s) 88, 548, 1489, 1582, 1651, 1802
HspAI GCGC 7 cut(s) 81, 96, 98, 112, 138, 1315, 1675
KasI GGCGCC 2 cut(s) 80, 137
KroI GCCGGC 2 cut(s) 134, 140
KroNI GCCGGC 2 cut(s) 136, 142
Ksp22I TGATCA 2 cut(s) 532, 841
Kzo9I GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
LmnI GCTCC 8 cut(s) 231, 239, 245, 512, 1101, 1162, 1419, 1828
Lsp1109I GCAGC 4 cut(s) 292, 300, 1054, 1253
LweI GCATC 1 cut(s) 1609
MaeI CTAG 2 cut(s) 713, 1395
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 295, 731
MalI GATC 7 cut(s) 224, 466, 534, 717, 843, 882, 1569
MbiI CCGCTC 2 cut(s) 218, 308
MboI GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
MflI RGATCY 3 cut(s) 464, 715, 880
MhlI GDGCHC 2 cut(s) 236, 1098
MluCI AATT 5 cut(s) 499, 670, 963, 1132, 1717
Mly113I GGCGCC 2 cut(s) 81, 138
MlyI GAGTC 5 cut(s) 66, 269, 580, 1525, 1564
MmeI TCCRAC 1 cut(s) 1110
MreI CGCCGGCG 1 cut(s) 134
MroNI GCCGGC 2 cut(s) 134, 140
MroXI GAANNNNTTC 1 cut(s) 1517
MseI TTAA 4 cut(s) 876, 1145, 1350, 1689
MslI CAYNNNNRTG 1 cut(s) 549
MspI CCGG 5 cut(s) 78, 135, 141, 237, 243
MspR9I CCNGG 4 cut(s) 70, 1444, 1833, 1881
Mva1269I GAATGC 1 cut(s) 1317
MvaI CCWGG 4 cut(s) 70, 1444, 1833, 1881
MvnI CGCG 2 cut(s) 96, 98
MwoI GCNNNNNNNGC 5 cut(s) 165, 1042, 1314, 1420, 1466
NaeI GCCGGC 2 cut(s) 136, 142
NarI GGCGCC 2 cut(s) 81, 138
NdeI CATATG 1 cut(s) 1636
NdeII GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
NgoMIV GCCGGC 2 cut(s) 134, 140
NlaIII CATG 6 cut(s) 88, 548, 1489, 1582, 1651, 1802
NlaIV GGNNCC 6 cut(s) 82, 139, 241, 508, 1097, 1830
NmeAIII GCCGAG 2 cut(s) 237, 1057
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 2 cut(s) 1489, 1582
PaeI GCATGC 1 cut(s) 1489
PagI TCATGA 1 cut(s) 544
PauI GCGCGC 1 cut(s) 96
PciI ACATGT 1 cut(s) 1578
PctI GAATGC 1 cut(s) 1317
PdiI GCCGGC 2 cut(s) 136, 142
PdmI GAANNNNTTC 1 cut(s) 1517
PfeI GAWTC 3 cut(s) 433, 763, 1073
PleI GAGTC 5 cut(s) 65, 268, 580, 1525, 1563
PluTI GGCGCC 2 cut(s) 84, 141
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 5 cut(s) 65, 268, 580, 1525, 1563
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 1211
PscI ACATGT 1 cut(s) 1578
PshBI ATTAAT 1 cut(s) 1350
PsiI TTATAA 2 cut(s) 743, 1014
Psp124BI GAGCTC 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 4 cut(s) 68, 1442, 1831, 1879
PspCI CACGTG 1 cut(s) 295
PspFI CCCAGC 2 cut(s) 51, 1423
PspGI CCWGG 4 cut(s) 68, 1442, 1831, 1879
PspN4I GGNNCC 6 cut(s) 82, 139, 241, 508, 1097, 1830
PspPI GGNCC 5 cut(s) 4, 193, 486, 996, 1211
PspPPI RGGWCCY 1 cut(s) 1211
PstI CTGCAG 2 cut(s) 201, 1271
PstNI CAGNNNCTG 1 cut(s) 1332
PsuI RGATCY 3 cut(s) 464, 715, 880
PteI GCGCGC 1 cut(s) 96
RsaI GTAC 2 cut(s) 178, 185
RsaNI GTAC 2 cut(s) 177, 184
RseI CAYNNNNRTG 1 cut(s) 549
SacI GAGCTC 1 cut(s) 236
SaqAI TTAA 4 cut(s) 876, 1145, 1350, 1689
Sau3AI GATC 7 cut(s) 222, 464, 532, 715, 841, 880, 1567
Sau96I GGNCC 5 cut(s) 4, 193, 486, 996, 1211
SchI GAGTC 5 cut(s) 66, 269, 580, 1525, 1564
ScrFI CCNGG 4 cut(s) 70, 1444, 1833, 1881
SduI GDGCHC 2 cut(s) 236, 1098
SfaNI GCATC 1 cut(s) 1609
SfcI CTRYAG 2 cut(s) 197, 1267
SfoI GGCGCC 2 cut(s) 82, 139
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 4 cut(s) 193, 486, 996, 1211
SmiMI CAYNNNNRTG 1 cut(s) 549
SmlI CTYRAG 3 cut(s) 437, 1231, 1496
SmoI CTYRAG 3 cut(s) 437, 1231, 1496
SphI GCATGC 1 cut(s) 1489
Sse9I AATT 5 cut(s) 499, 670, 963, 1132, 1717
SspDI GGCGCC 2 cut(s) 80, 137
SspI AATATT 1 cut(s) 1151
SspMI CTAG 2 cut(s) 713, 1395
SstI GAGCTC 1 cut(s) 236
StyD4I CCNGG 4 cut(s) 68, 1442, 1831, 1879
StyI CCWWGG 4 cut(s) 999, 1182, 1394, 1747
TaaI ACNGT 1 cut(s) 665
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 1529
TasI AATT 5 cut(s) 499, 670, 963, 1132, 1717
TauI GCSGC 7 cut(s) 45, 96, 162, 165, 168, 269, 311
TfiI GAWTC 3 cut(s) 433, 763, 1073
Tru1I TTAA 4 cut(s) 876, 1145, 1350, 1689
Tru9I TTAA 4 cut(s) 876, 1145, 1350, 1689
TscAI CASTG 4 cut(s) 670, 686, 1038, 1337
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 4 cut(s) 280, 288, 1042, 1266
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 5 cut(s) 827, 892, 1311, 1335, 1730
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 4 cut(s) 670, 686, 1038, 1337
VpaK11BI GGWCC 4 cut(s) 193, 486, 996, 1211
VspI ATTAAT 1 cut(s) 1350
XagI CCTNNNNNAGG 1 cut(s) 1878
XapI RAATTY 1 cut(s) 1717
XceI RCATGY 2 cut(s) 1489, 1582
XcmI CCANNNNNNNNNTGG 2 cut(s) 1450, 1805
XmaJI CCTAGG 1 cut(s) 1394
XmnI GAANNNNTTC 1 cut(s) 1517
XspI CTAG 2 cut(s) 713, 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.