Rh2DG094000

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015446
Physical Location & Seq
Reverse (-)
7389745 .. 7393928
4184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG094000.1

Sequence Viewer

Length: 1923 bp
ATGGGCCACCACCACCACCTCCACAACACCACCGCCGACGGCGGCGTCTCCCAGCGAGTCAACAGCCCCAGGTTCTCCGGCGCCATGACCCGCCGCGCCCACTCCTTCAAGCGCAACCCTTTCTCCTCCTCCGCCGGCGCCGGCCACAATGACGACGGCGGCGGCGGAAGTGGCGGCGGGTTCAGTACCCAGTACGAGGTGGACCTGCAGATGACCTCGCCCAGAGCGGAGATCGGCGGGAGCTCCGGCTCCGGCGAGGGGTTTGGTGAGTCGGCGGCGGAGAGGAAGCAGCAGAGCAGCCACGTGACGCAGAGGGCGGCGGTGAGAGGGTTTCTGAGGAAGCCGATTGAGGCGGTTATGGTGGAGATGGGTTTGAGGGAAAGGAAGAGACTTGGGCATTGGATGTTCTTTGCGTTTTGTGGGCTTTGTTTGTTTCTGGGGATTCTCAAGATTTGTGCTACTGGCTGGTTTGGATCTGCTATTGAAACTGCAAGGTCCAATCAGGATAATTCTGGCTCCACGGCTCATTCAAATCGGATTGATCAAAGCTCTCATGATTATGGGTATAGAGAGGGAGGAACTGATGTTGAACGGACTCTAATGATGGTATCGTCTGGTGTGGTTGGTGGAGAGAACAGAGCTGAATACACAGGTATCTGGTCCCAACCCAACAGTGCAAATTACACTCAGTGCATTGACCATCCAAAAAGCCACAAAAAGCTAGATCCAAAGACCAATGGTTACGTTCTTATAAATGCCAATGGTGGCTTGAATCAGATGAGATTTGGGATTTGTGATATGGTTGCTGTTGCTAAGATTATGAAGGCAACACTTGTCCTACCTTCACTTGATCACACCTCCTATTGGGCTGATGAGAGTGGCTTTAAAGATCTGTTTGATTGGCAACACTTCATTGAGACATTGAAGGATGATATCCACATAGTTGAGACATTACCACCTGAATATGCAGGAATTGAACCTTTCAACAAAACACCAATATCTTGGTCTAAGGCAAGTTATTATAAGTCAGAGGTTCTCCCACTGCTGAAGCAGCACACGGCAGTCTATCTCACTCATACTGATTCTCGGCTTTCCAACAATGGGCTTCCAAGTTCCATTCAAAAACTGAGGTGTCGTGTGAATTACAGGGCATTGAAATATTCAGCTCCAATAGAAGAACTTGGAAAAACCTTGGTTTCTAGAATGAGGCAGAATGGAGGTCCTTACCTTGCTCTCCACTTGAGGTATGAGAAGGATATGCTTGCATTTACGGGCTGCAGTCATAGTTTGACAGCAGAAGAGGATGAAGAACTACGCAGAATGCGCTATGAAGTCAGCCACTGGAAGGAGAAAGAGATTAATGGGACAGAGAGAAGGTTGCTTGGTGGTTGTCCATTGACCCCTAGGGAGACTTCTCTTTTGCTCCGAGGGCTGGGTTTTCCTTCAAGCACCAGGATTTACTTGGTAGCTGGTGAAGCTTATGGGAATGGTAGCATGCGACATCTTGAGGATGACTTCCCGAACATCTTCTCCCATTCGACTCTCGCCTCAGAAGAAGAACTGAGTCCATTCAGGGATCATCAGAACATGTTGGCTGGTATAGACTATGCTGTTGCCTTAGAGAGTGATGCTTTTCTTTATACATATGATGGAAACATGGCAAAAGCTGTTCAAGGGCATAGGCGCTTTGAGAACTTTAAGAAGACCATCAATCCAGACAAGATGAATTTTGTGAAACTTGTTGATGATTTTGACCAGGGAAAAATCTCTTGGAAGAAGTTTAGTTCTAAGGTGAAGAAGCTTCACCATGACAGAGATGGGTCTCCATATTTGAGGGAGCCTGGAGAGTTTCCGAAGCTGGAAGAGAGTTTCTATGCAAATCCTTTTCCAGGCTGTATTTGTGAAACAAGATGGAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

640

Amino Acids

71.69

Weight (kDa)

8.18

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 248 - 563 7.8e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35570
fragaria_vesca FvH4_1g08590
malus_domestica MD02G1090100.v1.1 MD15G1216700.v1.1
prunus_persica Prupe.7G199900_v2.0.a1
pyrus_communis pycom02g07200
rosa_chinensis RchiOBHm_Chr2g0094811
rosa_laevigata RLG00000016523
rosa_multiflora Rmu_sc0006422.1_g000026 Rmu_sc0011074.1_g000001
rosa_roxburghii Rroxscaffold_2G00146850
rosa_rugosa Rorug02G0047100
rosa_samantha Rh2AG094600 Rh2BG095200 Rh2CG097000 Rh2DG094000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 752, 1023
AasI GACNNNNNNGTC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 2 cut(s) 80, 137
AccBSI CCGCTC 1 cut(s) 227
AccII CGCG 1 cut(s) 96
AclWI GGATC 3 cut(s) 481, 719, 1584
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 1726
AcuI CTGAAG 1 cut(s) 1067
AcvI CACGTG 1 cut(s) 304
AcyI GRCGYC 3 cut(s) 45, 81, 138
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 2 cut(s) 187, 194
AfiI CCNNNNNNNGG 7 cut(s) 196, 258, 864, 1101, 1345, 1432, 1889
AflIII ACRYGT 1 cut(s) 1587
AjnI CCWGG 5 cut(s) 68, 1451, 1755, 1840, 1888
AjuI GAANNNNNNNTTGG 2 cut(s) 1753, 1785
AloI GAACNNNNNNTCC 2 cut(s) 1514, 1546
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 6 cut(s) 52, 382, 911, 941, 1403, 1827
AlwI GGATC 3 cut(s) 481, 719, 1584
AlwNI CAGNNNCTG 1 cut(s) 1341
AoxI GGCC 2 cut(s) 4, 142
ApeKI GCWGC 4 cut(s) 289, 297, 1051, 1275
ApoI RAATTY 1 cut(s) 1726
ArsI GACNNNNNNTTYG 2 cut(s) 1402, 1434
AseI ATTAAT 1 cut(s) 1359
Asp700I GAANNNNTTC 1 cut(s) 1526
AspA2I CCTAGG 1 cut(s) 1403
AspLEI GCGC 6 cut(s) 83, 98, 114, 140, 1326, 1686
AspS9I GGNCC 5 cut(s) 4, 202, 495, 660, 1220
AsuHPI GGTGA 5 cut(s) 278, 334, 1484, 1796, 1804
AvaII GGWCC 4 cut(s) 202, 495, 660, 1220
AvrII CCTAGG 1 cut(s) 1403
BaeI ACNNNNGTAYC 2 cut(s) 637, 670
BanI GGYRCC 2 cut(s) 80, 137
BanII GRGCYC 1 cut(s) 245
BbrPI CACGTG 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 1709
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 4 cut(s) 301, 309, 1063, 1262
BccI CCATC 7 cut(s) 361, 598, 708, 1643, 1715, 1811, 1905
BceAI ACGGC 4 cut(s) 55, 172, 537, 1074
BciT130I CCWGG 5 cut(s) 70, 1453, 1757, 1842, 1890
BclI TGATCA 2 cut(s) 541, 850
BcoDI GTCTC 6 cut(s) 52, 382, 911, 941, 1403, 1827
BfaI CTAG 3 cut(s) 722, 1200, 1404
BfmI CTRYAG 2 cut(s) 206, 1276
BfoI RGCGCY 3 cut(s) 84, 141, 1687
BfuAI ACCTGC 1 cut(s) 213
BglII AGATCT 1 cut(s) 889
BlnI CCTAGG 1 cut(s) 1403
Bme1390I CCNGG 5 cut(s) 70, 1453, 1757, 1842, 1890
Bme18I GGWCC 4 cut(s) 202, 495, 660, 1220
BmgT120I GGNCC 5 cut(s) 4, 202, 495, 660, 1220
BmiI GGNNCC 6 cut(s) 82, 139, 250, 517, 662, 1839
BmrFI CCNGG 5 cut(s) 70, 1453, 1757, 1842, 1890
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 1618
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 1709
BpmI CTGGAG 1 cut(s) 1863
BpuEI CTTGAG 3 cut(s) 431, 1261, 1526
BsaAI YACGTR 1 cut(s) 304
BsaHI GRCGYC 3 cut(s) 45, 81, 138
BsaI GGTCTC 1 cut(s) 1827
BsaJI CCNNGG 6 cut(s) 68, 519, 1191, 1403, 1426, 1756
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 1514, 1544
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 258, 864, 1101, 1345, 1432, 1889
Bse118I RCCGGY 2 cut(s) 134, 140
Bse1I ACTGG 3 cut(s) 190, 466, 1346
BseBI CCWGG 5 cut(s) 70, 1453, 1757, 1842, 1890
BseDI CCNNGG 6 cut(s) 68, 519, 1191, 1403, 1426, 1756
BseGI GGATG 5 cut(s) 408, 700, 934, 1309, 1516
BseLI CCNNNNNNNGG 7 cut(s) 196, 258, 864, 1101, 1345, 1432, 1889
BseMII CTCAG 5 cut(s) 326, 701, 1118, 1553, 1563
BseNI ACTGG 3 cut(s) 190, 466, 1346
BseRI GAGGAG 2 cut(s) 115, 118
BseXI GCAGC 4 cut(s) 301, 309, 1063, 1262
BseYI CCCAGC 2 cut(s) 51, 1432
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 6, 144
BshNI GGYRCC 2 cut(s) 80, 137
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 5 cut(s) 78, 135, 141, 246, 252
BslFI GGGAC 2 cut(s) 646, 1378
BslI CCNNNNNNNGG 7 cut(s) 196, 258, 864, 1101, 1345, 1432, 1889
BsmAI GTCTC 6 cut(s) 52, 382, 911, 941, 1403, 1827
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 646, 1378
BsmI GAATGC 1 cut(s) 1326
BsnI GGCC 2 cut(s) 6, 144
Bso31I GGTCTC 1 cut(s) 1827
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
BspANI GGCC 2 cut(s) 6, 144
BspCNI CTCAG 5 cut(s) 327, 700, 1119, 1554, 1562
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 6 cut(s) 82, 139, 250, 517, 662, 1839
BspMAI CTGCAG 2 cut(s) 210, 1280
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 481, 719, 1584
BspT107I GGYRCC 2 cut(s) 80, 137
BspTNI GGTCTC 1 cut(s) 1827
BsrBI CCGCTC 1 cut(s) 227
BsrFI RCCGGY 2 cut(s) 134, 140
BsrI ACTGG 3 cut(s) 190, 466, 1346
BssAI RCCGGY 2 cut(s) 134, 140
BssECI CCNNGG 6 cut(s) 68, 519, 1191, 1403, 1426, 1756
BssMI GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
BssNI GRCGYC 3 cut(s) 45, 81, 138
BssT1I CCWWGG 2 cut(s) 1191, 1403
Bst2UI CCWGG 5 cut(s) 70, 1453, 1757, 1842, 1890
Bst4CI ACNGT 1 cut(s) 674
Bst6I CTCTTC 3 cut(s) 380, 1293, 1857
BstACI GRCGYC 3 cut(s) 45, 81, 138
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 4 cut(s) 136, 142, 1263, 1496
BstDEI CTNAG 9 cut(s) 335, 687, 813, 1008, 1127, 1549, 1562, 1618, 1788
BstDSI CCRYGG 1 cut(s) 519
BstENI CCTNNNNNAGG 1 cut(s) 1887
BstF5I GGATG 5 cut(s) 408, 700, 934, 1309, 1516
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 3 cut(s) 84, 141, 1687
BstHHI GCGC 6 cut(s) 83, 98, 114, 140, 1326, 1686
BstKTI GATC 7 cut(s) 234, 476, 544, 727, 853, 892, 1579
BstMAI GTCTC 6 cut(s) 52, 382, 911, 941, 1403, 1827
BstMBI GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
BstMWI GCNNNNNNNGC 5 cut(s) 171, 1051, 1323, 1429, 1475
BstNI CCWGG 5 cut(s) 70, 1453, 1757, 1842, 1890
BstNSI RCATGY 2 cut(s) 1498, 1591
BstSCI CCNGG 5 cut(s) 68, 1451, 1755, 1840, 1888
BstSFI CTRYAG 2 cut(s) 206, 1276
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 301, 309, 1063, 1262
BstV2I GAAGAC 1 cut(s) 1709
BstX2I RGATCY 3 cut(s) 473, 724, 889
BstXI CCANNNNNNTGG 1 cut(s) 1002
BstYI RGATCY 3 cut(s) 473, 724, 889
BsuRI GGCC 2 cut(s) 6, 144
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 5 cut(s) 408, 700, 934, 1309, 1516
BtsI GCAGTG 1 cut(s) 1040
BtsIMutI CAGTG 4 cut(s) 679, 695, 1040, 1339
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 4 cut(s) 136, 142, 1263, 1496
CaiI CAGNNNCTG 1 cut(s) 1341
CciI TCATGA 1 cut(s) 553
CfoI GCGC 6 cut(s) 83, 98, 114, 140, 1326, 1686
Cfr10I RCCGGY 2 cut(s) 134, 140
Cfr13I GGNCC 5 cut(s) 4, 202, 495, 660, 1220
CseI GACGC 2 cut(s) 34, 316
Csp6I GTAC 2 cut(s) 186, 193
CviAII CATG 6 cut(s) 85, 554, 1495, 1588, 1657, 1808
CviQI GTAC 2 cut(s) 186, 193
DdeI CTNAG 9 cut(s) 335, 687, 813, 1008, 1127, 1549, 1562, 1618, 1788
DinI GGCGCC 2 cut(s) 82, 139
DpnI GATC 7 cut(s) 233, 475, 543, 726, 852, 891, 1578
DpnII GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
DraI TTTAAA 1 cut(s) 886
DraIII CACNNNGTG 1 cut(s) 690
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 380, 1293, 1857
EarI CTCTTC 3 cut(s) 380, 1293, 1857
EciI GGCGGA 3 cut(s) 121, 180, 293
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 1191, 1403
Eco24I GRGCYC 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 1827
Eco32I GATATC 1 cut(s) 934
Eco47I GGWCC 4 cut(s) 202, 495, 660, 1220
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 1067
Eco72I CACGTG 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 1887
EcoO109I RGGNCCY 1 cut(s) 1220
EcoRII CCWGG 5 cut(s) 68, 1451, 1755, 1840, 1888
EcoRV GATATC 1 cut(s) 934
EcoT14I CCWWGG 2 cut(s) 1191, 1403
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 2 cut(s) 82, 139
EheI GGCGCC 2 cut(s) 82, 139
ErhI CCWWGG 2 cut(s) 1191, 1403
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 6 cut(s) 88, 557, 1498, 1591, 1660, 1811
FaqI GGGAC 2 cut(s) 646, 1378
FatI CATG 6 cut(s) 84, 553, 1494, 1587, 1656, 1807
FauI CCCGC 3 cut(s) 98, 170, 230
FauNDI CATATG 1 cut(s) 1645
FbaI TGATCA 2 cut(s) 541, 850
FokI GGATG 5 cut(s) 415, 687, 941, 1316, 1523
FriOI GRGCYC 1 cut(s) 245
FspBI CTAG 3 cut(s) 722, 1200, 1404
GlaI GCGC 6 cut(s) 82, 97, 113, 139, 1325, 1685
GsaI CCCAGC 2 cut(s) 55, 1436
GsuI CTGGAG 1 cut(s) 1863
HaeII RGCGCY 3 cut(s) 84, 141, 1687
HaeIII GGCC 2 cut(s) 6, 144
HapII CCGG 5 cut(s) 78, 135, 141, 246, 252
HgaI GACGC 2 cut(s) 34, 316
HhaI GCGC 6 cut(s) 83, 98, 114, 140, 1326, 1686
Hin1I GRCGYC 3 cut(s) 45, 81, 138
Hin1II CATG 6 cut(s) 88, 557, 1498, 1591, 1660, 1811
Hin6I GCGC 6 cut(s) 81, 96, 112, 138, 1324, 1684
HinP1I GCGC 6 cut(s) 81, 96, 112, 138, 1324, 1684
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 2 cut(s) 1476, 1799
HinfI GANTC 8 cut(s) 57, 269, 442, 595, 772, 1082, 1540, 1564
HpaII CCGG 5 cut(s) 78, 135, 141, 246, 252
HphI GGTGA 5 cut(s) 278, 334, 1484, 1796, 1804
Hpy166II GTNNAC 2 cut(s) 61, 202
Hpy188I TCNGA 8 cut(s) 336, 537, 777, 1030, 1427, 1552, 1584, 1854
Hpy188III TCNNGA 7 cut(s) 448, 503, 554, 1200, 1505, 1519, 1715
Hpy8I GTNNAC 2 cut(s) 61, 202
Hpy99I CGWCG 2 cut(s) 41, 158
HpyAV CCTTC 8 cut(s) 115, 817, 852, 919, 1246, 1339, 1368, 1452
HpyCH4III ACNGT 1 cut(s) 674
HpyCH4IV ACGT 2 cut(s) 303, 744
HpyCH4V TGCA 8 cut(s) 208, 491, 677, 693, 968, 1265, 1278, 1877
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 1051, 1323, 1429, 1475
HpyF3I CTNAG 9 cut(s) 335, 687, 813, 1008, 1127, 1549, 1562, 1618, 1788
HpySE526I ACGT 2 cut(s) 303, 744
Hsp92I GRCGYC 3 cut(s) 45, 81, 138
Hsp92II CATG 6 cut(s) 88, 557, 1498, 1591, 1660, 1811
HspAI GCGC 6 cut(s) 81, 96, 112, 138, 1324, 1684
KasI GGCGCC 2 cut(s) 80, 137
KroI GCCGGC 2 cut(s) 134, 140
KroNI GCCGGC 2 cut(s) 136, 142
Ksp22I TGATCA 2 cut(s) 541, 850
Kzo9I GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
LmnI GCTCC 7 cut(s) 240, 248, 254, 521, 1171, 1428, 1837
Lsp1109I GCAGC 4 cut(s) 301, 309, 1063, 1262
LweI GCATC 1 cut(s) 1618
MaeI CTAG 3 cut(s) 722, 1200, 1404
MaeII ACGT 2 cut(s) 303, 744
MaeIII GTNAC 2 cut(s) 304, 740
MalI GATC 7 cut(s) 233, 475, 543, 726, 852, 891, 1578
MbiI CCGCTC 1 cut(s) 227
MboI GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
MflI RGATCY 3 cut(s) 473, 724, 889
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 5 cut(s) 508, 679, 972, 1141, 1726
Mly113I GGCGCC 2 cut(s) 81, 138
MlyI GAGTC 5 cut(s) 66, 278, 589, 1534, 1573
MmeI TCCRAC 1 cut(s) 1119
MreI CGCCGGCG 1 cut(s) 134
MroNI GCCGGC 2 cut(s) 134, 140
MroXI GAANNNNTTC 1 cut(s) 1526
MseI TTAA 3 cut(s) 885, 1359, 1698
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 5 cut(s) 78, 135, 141, 246, 252
MspR9I CCNGG 5 cut(s) 70, 1453, 1757, 1842, 1890
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 5 cut(s) 70, 1453, 1757, 1842, 1890
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 5 cut(s) 171, 1051, 1323, 1429, 1475
NaeI GCCGGC 2 cut(s) 136, 142
NarI GGCGCC 2 cut(s) 81, 138
NdeI CATATG 1 cut(s) 1645
NdeII GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
NgoMIV GCCGGC 2 cut(s) 134, 140
NlaIII CATG 6 cut(s) 88, 557, 1498, 1591, 1660, 1811
NlaIV GGNNCC 6 cut(s) 82, 139, 250, 517, 662, 1839
NmeAIII GCCGAG 1 cut(s) 1066
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 2 cut(s) 1498, 1591
PaeI GCATGC 1 cut(s) 1498
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 1587
PctI GAATGC 1 cut(s) 1326
PdiI GCCGGC 2 cut(s) 136, 142
PdmI GAANNNNTTC 1 cut(s) 1526
PfeI GAWTC 3 cut(s) 442, 772, 1082
PleI GAGTC 5 cut(s) 65, 277, 589, 1534, 1572
PluTI GGCGCC 2 cut(s) 84, 141
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 5 cut(s) 65, 277, 589, 1534, 1572
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 1220
PscI ACATGT 1 cut(s) 1587
PshBI ATTAAT 1 cut(s) 1359
PsiI TTATAA 2 cut(s) 752, 1023
Psp124BI GAGCTC 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 1220
Psp6I CCWGG 5 cut(s) 68, 1451, 1755, 1840, 1888
PspCI CACGTG 1 cut(s) 304
PspFI CCCAGC 2 cut(s) 51, 1432
PspGI CCWGG 5 cut(s) 68, 1451, 1755, 1840, 1888
PspN4I GGNNCC 6 cut(s) 82, 139, 250, 517, 662, 1839
PspPI GGNCC 5 cut(s) 4, 202, 495, 660, 1220
PspPPI RGGWCCY 1 cut(s) 1220
PstI CTGCAG 2 cut(s) 210, 1280
PstNI CAGNNNCTG 1 cut(s) 1341
PsuI RGATCY 3 cut(s) 473, 724, 889
RsaI GTAC 2 cut(s) 187, 194
RsaNI GTAC 2 cut(s) 186, 193
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 885, 1359, 1698
Sau3AI GATC 7 cut(s) 231, 473, 541, 724, 850, 889, 1576
Sau96I GGNCC 5 cut(s) 4, 202, 495, 660, 1220
SchI GAGTC 5 cut(s) 66, 278, 589, 1534, 1573
ScrFI CCNGG 5 cut(s) 70, 1453, 1757, 1842, 1890
SduI GDGCHC 1 cut(s) 245
SfaNI GCATC 1 cut(s) 1618
SfcI CTRYAG 2 cut(s) 206, 1276
SfoI GGCGCC 2 cut(s) 82, 139
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 4 cut(s) 202, 495, 660, 1220
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 3 cut(s) 446, 1240, 1505
SmoI CTYRAG 3 cut(s) 446, 1240, 1505
SphI GCATGC 1 cut(s) 1498
Sse9I AATT 5 cut(s) 508, 679, 972, 1141, 1726
SspDI GGCGCC 2 cut(s) 80, 137
SspI AATATT 1 cut(s) 1160
SspMI CTAG 3 cut(s) 722, 1200, 1404
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 5 cut(s) 68, 1451, 1755, 1840, 1888
StyI CCWWGG 2 cut(s) 1191, 1403
TaaI ACNGT 1 cut(s) 674
TaiI ACGT 2 cut(s) 306, 747
TaqI TCGA 1 cut(s) 1538
TasI AATT 5 cut(s) 508, 679, 972, 1141, 1726
TauI GCSGC 7 cut(s) 45, 96, 162, 165, 177, 278, 320
TfiI GAWTC 3 cut(s) 442, 772, 1082
Tru1I TTAA 3 cut(s) 885, 1359, 1698
Tru9I TTAA 3 cut(s) 885, 1359, 1698
TscAI CASTG 4 cut(s) 679, 695, 1047, 1346
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 289, 297, 1051, 1275
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 5 cut(s) 836, 901, 1320, 1344, 1739
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 4 cut(s) 679, 695, 1047, 1346
VpaK11BI GGWCC 4 cut(s) 202, 495, 660, 1220
VspI ATTAAT 1 cut(s) 1359
XagI CCTNNNNNAGG 1 cut(s) 1887
XapI RAATTY 1 cut(s) 1726
XbaI TCTAGA 1 cut(s) 1199
XceI RCATGY 2 cut(s) 1498, 1591
XcmI CCANNNNNNNNNTGG 2 cut(s) 1459, 1814
XmaJI CCTAGG 1 cut(s) 1403
XmnI GAANNNNTTC 1 cut(s) 1526
XspI CTAG 3 cut(s) 722, 1200, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.