MD02G1106300.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009582
Physical Location & Seq
Forward (+)
8603057 .. 8611583
8527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1106300.v1.1.491

Sequence Viewer

Length: 3819 bp
ATGACAATGGACCTGCACCCGTCAATGGCGCAAAGTGTGAGAACAAGTAGGTCTTCTTTTAGCTCTAGCAATGGCAATGAGAATACAACTCCTTTACACAGCTCTGCTGCTGTTGCATTTGCAAATGGGGATGATTATGACAGTGACAGCTCCAGCTTGGCACCGCAAACACCAAGGACCTTGTCCATGGACATCCCAGCAGAACTTGCTGGTGCAATACCCTTGATTGATAGATTTCAGGTAGAAGGATTTTTGAGGTTGATGCAGAAACAGATTCAGTCTGCTGGAAAACGTGGATTCTTTACAAAAAAATCCATTGGTCCTCAACCCCGAGAGAGGTTCACTTTTGAGGATATGCTGTGCTACCAAAAGGATCCAATACCTACGTCATTACTGAAAATCAATAGTGACCTGGTAAGCCGGGCAATGAAACTGTTCCAAATCATTTTGAAGTACATGGGAGTTGATTCATCTGATGATCGAGTTACTCCAGCAAGCTTAGATGAACGGGTAGAGCTTGTTGGAAAGATGTTTAAGCAAACCTTGAAGCGGACAGAGCTTCGGGATGAGCTTTTTGTTCAGATTTCAAAACAAACAAGAAATAATCCCGATAAGCAATATTTGATCAAAGCATGGGAATTAATGTATCTGTGTTCATCCTCTATGCCTCCGAGCAAGGACATTGGGGGGTATTTGTCCGAGTATGTCCACAATGTAGCACATGGTGTGAATATTGACTCTGAGGTTCAAGTCCTAGCAATTAATACATTAAATGCTTTAAAGCGTTCTGTAAAGGCTGGTCCTAGACATACAATACCAGGCCGCGAAGAGATTGAAGCGCTTTTAACTGGTCGAAAGCTAACAACAATTGTGTTTTTCTTGGATGAAACCTTTGAAGAAATCACATATGATATGGCAACAACAGTGGCTGATGCTGTTGAGGAACTTGCAGGGGTAATTAAACTCTCAGCTCCTTCTAGCTTCAGTCTGTTTGAATGCCGTAAAGTTGTAACTGGCTCAAAATCACCTGATCCTGGAAATGAGGAGTATATTGGGTTAGATGACAACAAGTACATAGGGGATCTATTAGCAGAACTCAAGGCCGCAAAGGATCGAAGTAAAGGAGAAATATTGCATTGCAAGCTGACATTTAAGAAAAAGCTGTTTCGGGAGTCGGATGAAGCTGTCACTGATCCAATGTTTGTGCAGTTGTCCTATGTTCAGTTGCAACATGATTATATACTGGGAAATTATCCTGTCGGAAGAGATGATGCTGCCCAGCTGTCTGCATTGCAAATCTTGGTTGACATCGGATTTCTGCGCAATCCTGAATCATGCACTGACTGGAATTTGCTTTTGGAACGCTTCCTACCCAGACAAATCGCAATCACACGAGCAAAACGAGAGTGGGAGTTTGATATTCTTTCTCGTTACCATTCAATGGAAAATTTGACAAAAGATGATGCAAGACAACAATTTTTGAGAATACTGAGACAACTTCCTTACGGGAATTCAGTCTTCTTCAGTGTTCGCAAGATTGATGATCCCATTGGACTTCTTCCAGGGAAAATTATTTTGGGCATTAACAAAAGAGGGGTTCATTTTTTCCGTCCAGTTCCAAAGGAATATCTACATTCAGCTGAGCTAAGAGACATCATGCAATTTGGTAGTAGCAACACTGCTGTTTTCTTTAAGATGAGAGTTGCAGGTGTTCTTCACATTTTCCAGTTTGAAACAAAGCAGGGTGAAGAAATTTGTGTTGCTCTTCAAACACACATAAATGATGTCATGTTGAGGCGCTATTCTAAAGCTCGGCCTGCTGCTAGTGGTTCAACAAACGGAGATCTTTCTAACAATGTTAAGCCCTCTGATGGTGAAGTTTATGAAAAACGTGTACAGCATTTGTCAAAAGCTGTTGAAGAGTCTCAGAGGAATGCTGATCAATTGCTGGAAGAATTGCGTGAAAAGCAAAAACAAGAAGCAAACATGCAAGAAGAATTAGAAAGTTTGAAGCAATCCTTGGCATCTGAGAAGCAAAACCTGACAGAAGTTAGATGTGATCATGATAGGCTTAGATTATTGTGTGATGAAAAGGATATGGCACTTCAGGCTGCACTACTAGAGAAAAAAAGCGTGGAAGCAAGGCTGCAAAACTTGGGTAATCAATTGGCAGAGAAAAATAACAAAACACAGCAAGTTGGTGGAAATAACCACGCATTGCAGAAGCTCGAAGACGAAATAAAGCTTCGTGTTGAGGAGTTGTTAGCAAAAGAAAAAACTATAAGGAGACTATCTGATGAAAAAATATCATTGGAGCAAAGACTTTCTGGGCTCAAAAAGACTAAATCTGTTGAGATTGATTCTGTTGAGAAAAAATTTGAGCAAGAGCGTAGAGCGTTAAAGCTTCAAGTGTTTGAACTTGAAAAGAAGCTTGAAGGAGTTAACCAAGAATTAGCAGGTCTTAAGTCAACTCTTGCCACCAAAAACTCTGAGGTTGCTGAATTACAAAGTAGCTTAAAGGAATTGGAGGAACTGAGAGAAATGAAAGAGGACATTGATAGAAAGAACGAGCAAACTGCTGCCATCTTGAGGATGCAAGGAGCTCAGCTAGCTGAGATGGAAGCGCTTTACAAGGAAGAACAACTTTTGAGGAAGCGATATTTTAATACTATAGAAGATATGAAAGGCAAGATTAGAGTCTTCTGTCGATTAAGACCTTTGAGTGAAAAAGAAATTACAGAAAAGGAAAGAGATACAGTTAAAAGTGTAGATGAGTTCACAATTGAACATCCATGGAAAGATGACAAACTGAAACAACATATGTATGATCGTGTATTTGATGGTAATGCCACCCAAGAGGATGTCTTTGAAGATACCAGGTATTTGGTACAATCTGCAGTTGATGGGTATAATGTGTGCATATTTGCATATGGGCAAACCGGTTCCGGAAAGACATATACCATATACGGTTCAGAAACTAATCCTGGACTTACTCCACGAGCTACTGCAGAGCTGTTTAAAATATTGAGGCGAGAGAACAACAAATTCTCATTTTCGTTAAAGGCATACATGTTGGAGTTGTATCAAGATACACTTGTAGACCTTCTCTTACCGAAAAATGGCAAGCGTTTGAAATTGGAGATCAAGAAAGATTCAAAGGGTATGGTCTCGGTTGAGAATGTAACAGTTCTCTCAATTTCATCACATGACGAACTGAAAAGTGTCATTCAAAGAGGTTCTGAACGAAGGCATACTGCTGGAACTCAGATGAACGATGAAAGTTCAAGATCACACCTCATAGTTTCTGTCGTCATCGAAAGTACCAACCTTCAAACCCAATCTGTTGCAAGAGGAAAGCTAAGTTTTGTGGATCTTGCTGGTTCGGAAAGAATAAAGAAGTCGGGCTCTTCAGGTAGTCAACTTAAAGAAGCGCAAAGCATCAACAAATCGCTTTCAGCACTTGGGGATGTTATTAGTTCTTTATCTTCCGGTGGTCAACACATACCGTACAGGAACCATAAGTTAACGATGCTTATGAGTGATTCACTTGGTGGCAACGCCAAGACGCTTATGTTTGTAAACGTTTCTCCTGCTGAATCAAATGTGGACGAGACGTACAATTCTCTAATGTATGCATCAAGAGTTCGATCAATCGTGAATGATCCAAGCAAAAACGTTTCTTCAAAAGAAATCATGCGGCTGAAGAAGTTGGTTGCATATTGGAAAGAACAAGCAGGCAAGAGAGAAGACGACGAAGATTTGGAAGAAATTCAGGATGAGCGACCTGTGAAAGATAGAGATGGCCGCCATTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

1273

Amino Acids

144.25

Weight (kDa)

6.61

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 176 - 280 9.8e-28 MyTH4 domain
RA_2 PF21989 286 - 335 2e-06 RA like domain
FERM_M PF00373 392 - 508 5.7e-22 FERM central domain
IRS PF02174 518 - 594 8.6e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 878 - 1036 1.3e-46 Microtubule binding
Kinesin PF00225 903 - 1218 2.2e-109 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1697
Acc16I TGCGCA 1 cut(s) 1322
Acc36I ACCTGC 3 cut(s) 21, 1697, 2447
AccB1I GGYRCC 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 1441
AccI GTMKAC 1 cut(s) 3099
AccII CGCG 1 cut(s) 825
AccIII TCCGGA 1 cut(s) 2945
AciI CCGC 6 cut(s) 164, 550, 823, 1104, 3697, 3805
AclI AACGTT 2 cut(s) 3582, 3675
AclWI GGATC 9 cut(s) 368, 381, 1025, 1089, 1119, 1187, 1538, 3378, 3656
AcoI YGGCCR 1 cut(s) 3802
AcsI RAATTY 7 cut(s) 1348, 1447, 1510, 1752, 2375, 3044, 3768
AcuI CTGAAG 5 cut(s) 967, 1507, 2090, 3393, 3722
AdeI CACNNNGTG 2 cut(s) 725, 3551
AfaI GTAC 7 cut(s) 455, 1073, 1896, 2889, 3322, 3509, 3617
AfeI AGCGCT 2 cut(s) 840, 2625
AfiI CCNNNNNNNGG 8 cut(s) 25, 336, 549, 1034, 1441, 1871, 2589, 3119
AflII CTTAAG 1 cut(s) 2462
AflIII ACRYGT 2 cut(s) 1891, 3069
AgeI ACCGGT 1 cut(s) 2939
AjnI CCWGG 6 cut(s) 411, 817, 1033, 1561, 2876, 2983
AjuI GAANNNNNNNTTGG 6 cut(s) 1340, 1372, 1559, 1591, 2003, 2035
Alw21I GWGCWC 1 cut(s) 2605
Alw26I GTCTC 6 cut(s) 1486, 1644, 1929, 2281, 3172, 3605
AlwI GGATC 9 cut(s) 368, 381, 1025, 1089, 1119, 1187, 1538, 3378, 3656
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 1 cut(s) 330
Aor13HI TCCGGA 1 cut(s) 2945
Aor51HI AGCGCT 2 cut(s) 840, 2625
AoxI GGCC 4 cut(s) 820, 1101, 1814, 3802
ApeKI GCWGC 6 cut(s) 107, 1274, 1820, 2111, 2146, 2579
ApoI RAATTY 7 cut(s) 1348, 1447, 1510, 1752, 2375, 3044, 3768
AseI ATTAAT 2 cut(s) 641, 762
AsiGI ACCGGT 1 cut(s) 2939
Asp700I GAANNNNTTC 2 cut(s) 434, 3768
AspLEI GCGC 6 cut(s) 31, 841, 1323, 1800, 2626, 3433
AspS9I GGNCC 4 cut(s) 10, 177, 320, 800
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 1017, 1757, 1886
AsuNHI GCTAGC 1 cut(s) 2608
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 4 cut(s) 10, 177, 320, 800
BaeI ACNNNNGTAYC 2 cut(s) 3081, 3114
BamHI GGATCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 3 cut(s) 2334, 2605, 3407
BauI CACGAG 2 cut(s) 1392, 2997
BbsI GAAGAC 5 cut(s) 45, 1510, 2238, 2692, 3753
Bbv12I GWGCWC 1 cut(s) 2605
BbvI GCAGC 6 cut(s) 94, 1261, 1807, 2098, 2133, 2566
BccI CCATC 6 cut(s) 1865, 2591, 2611, 2834, 2897, 3794
BceAI ACGGC 1 cut(s) 984
BcgI CGANNNNNNTGC 2 cut(s) 2558, 2592
BciT130I CCWGG 6 cut(s) 413, 819, 1035, 1563, 2878, 2985
BclI TGATCA 3 cut(s) 624, 1939, 2059
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 6 cut(s) 1486, 1644, 1929, 2281, 3172, 3605
BfaI CTAG 7 cut(s) 66, 755, 804, 978, 1824, 2120, 2609
BfmI CTRYAG 3 cut(s) 2670, 2895, 3006
BfoI RGCGCY 3 cut(s) 842, 1801, 2627
BfrI CTTAAG 1 cut(s) 2462
BfuAI ACCTGC 3 cut(s) 21, 1697, 2447
BglII AGATCT 1 cut(s) 1843
BlpI GCTNAGC 2 cut(s) 1641, 2604
Bme1390I CCNGG 7 cut(s) 413, 422, 819, 1035, 1563, 2878, 2985
Bme18I GGWCC 4 cut(s) 10, 177, 320, 800
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 4 cut(s) 10, 177, 320, 800
BmiI GGNNCC 4 cut(s) 162, 375, 2944, 3515
BmrFI CCNGG 7 cut(s) 413, 422, 819, 1035, 1563, 2878, 2985
BmrI ACTGGG 1 cut(s) 1253
BmsI GCATC 9 cut(s) 252, 922, 1261, 1453, 2033, 2583, 3447, 3519, 3644
BmtI GCTAGC 1 cut(s) 2612
BmuI ACTGGG 1 cut(s) 1253
BpiI GAAGAC 5 cut(s) 45, 1510, 2238, 2692, 3753
BpmI CTGGAG 2 cut(s) 136, 474
Bpu1102I GCTNAGC 2 cut(s) 1641, 2604
BpuEI CTTGAG 2 cut(s) 1082, 2608
BpuMI CCSGG 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 3172
BsaJI CCNNGG 5 cut(s) 173, 186, 1562, 2019, 2792
BsaWI WCCGGW 3 cut(s) 2939, 2945, 3488
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 336, 549, 1034, 1441, 1871, 2589, 3119
Bse118I RCCGGY 1 cut(s) 2939
Bse1I ACTGG 6 cut(s) 853, 1018, 1248, 1349, 1613, 1726
Bse3DI GCAATG 6 cut(s) 76, 82, 432, 1135, 1289, 2216
BseAI TCCGGA 1 cut(s) 2945
BseBI CCWGG 6 cut(s) 413, 819, 1035, 1563, 2878, 2985
BseDI CCNNGG 5 cut(s) 173, 186, 1562, 2019, 2792
BseLI CCNNNNNNNGG 8 cut(s) 25, 336, 549, 1034, 1441, 1871, 2589, 3119
BseMI GCAATG 6 cut(s) 76, 82, 432, 1135, 1289, 2216
BseNI ACTGG 6 cut(s) 853, 1018, 1248, 1349, 1613, 1726
BseRI GAGGAG 2 cut(s) 1058, 2270
BseXI GCAGC 6 cut(s) 94, 1261, 1807, 2098, 2133, 2566
BseYI CCCAGC 2 cut(s) 196, 1278
BsgI GTGCAG 2 cut(s) 1226, 2097
Bsh1236I CGCG 1 cut(s) 825
BshFI GGCC 4 cut(s) 822, 1103, 1816, 3804
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 2939
BsiHKAI GWGCWC 1 cut(s) 2605
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 4 cut(s) 421, 2940, 2946, 3489
BslI CCNNNNNNNGG 8 cut(s) 25, 336, 549, 1034, 1441, 1871, 2589, 3119
BsmAI GTCTC 6 cut(s) 1486, 1644, 1929, 2281, 3172, 3605
BsmBI CGTCTC 1 cut(s) 3605
BsmI GAATGC 2 cut(s) 1001, 1939
BsnI GGCC 4 cut(s) 822, 1103, 1816, 3804
Bso31I GGTCTC 1 cut(s) 3172
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 2334, 2605, 3407
Bsp13I TCCGGA 1 cut(s) 2945
Bsp1407I TGTACA 1 cut(s) 1894
Bsp1720I GCTNAGC 2 cut(s) 1641, 2604
Bsp19I CCATGG 2 cut(s) 186, 2792
BspACI CCGC 6 cut(s) 164, 550, 823, 1104, 3697, 3805
BspANI GGCC 4 cut(s) 822, 1103, 1816, 3804
BspEI TCCGGA 1 cut(s) 2945
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 1 cut(s) 2062
BspLI GGNNCC 4 cut(s) 162, 375, 2944, 3515
BspMAI CTGCAG 2 cut(s) 2899, 3010
BspMI ACCTGC 3 cut(s) 21, 1697, 2447
BspOI GCTAGC 1 cut(s) 2612
BspPI GGATC 9 cut(s) 368, 381, 1025, 1089, 1119, 1187, 1538, 3378, 3656
BspQI GCTCTTC 2 cut(s) 1770, 3412
BspT107I GGYRCC 1 cut(s) 160
BspTI CTTAAG 1 cut(s) 2462
BspTNI GGTCTC 1 cut(s) 3172
BsrDI GCAATG 6 cut(s) 76, 82, 432, 1135, 1289, 2216
BsrFI RCCGGY 1 cut(s) 2939
BsrGI TGTACA 1 cut(s) 1894
BsrI ACTGG 6 cut(s) 853, 1018, 1248, 1349, 1613, 1726
BssAI RCCGGY 1 cut(s) 2939
BssECI CCNNGG 5 cut(s) 173, 186, 1562, 2019, 2792
BssSI CACGAG 2 cut(s) 1392, 2997
BssT1I CCWWGG 4 cut(s) 173, 186, 2019, 2792
Bst2BI CACGAG 2 cut(s) 1392, 2997
Bst2UI CCWGG 6 cut(s) 413, 819, 1035, 1563, 2878, 2985
Bst4CI ACNGT 7 cut(s) 143, 435, 925, 2758, 2969, 3187, 3507
Bst6I CTCTTC 5 cut(s) 822, 1258, 1770, 1914, 3412
BstAFI CTTAAG 1 cut(s) 2462
BstAPI GCANNNNNTGC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 1894
BstC8I GCNNGC 6 cut(s) 496, 1142, 1818, 2610, 3125, 3736
BstDSI CCRYGG 2 cut(s) 186, 2792
BstFNI CGCG 1 cut(s) 825
BstH2I RGCGCY 3 cut(s) 842, 1801, 2627
BstHHI GCGC 6 cut(s) 31, 841, 1323, 1800, 2626, 3433
BstMAI GTCTC 6 cut(s) 1486, 1644, 1929, 2281, 3172, 3605
BstMWI GCNNNNNNNGC 8 cut(s) 113, 206, 556, 1141, 1817, 1966, 2108, 2609
BstNI CCWGG 6 cut(s) 413, 819, 1035, 1563, 2878, 2985
BstNSI RCATGY 2 cut(s) 1990, 3073
BstSCI CCNGG 7 cut(s) 411, 420, 817, 1033, 1561, 2876, 2983
BstSFI CTRYAG 3 cut(s) 2670, 2895, 3006
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 6 cut(s) 94, 1261, 1807, 2098, 2133, 2566
BstV2I GAAGAC 5 cut(s) 45, 1510, 2238, 2692, 3753
BstX2I RGATCY 4 cut(s) 373, 1081, 1843, 3370
BstXI CCANNNNNNTGG 1 cut(s) 2884
BstYI RGATCY 4 cut(s) 373, 1081, 1843, 3370
BsuRI GGCC 4 cut(s) 822, 1103, 1816, 3804
BtgI CCRYGG 2 cut(s) 186, 2792
BtsI GCAGTG 1 cut(s) 1677
BtsIMutI CAGTG 6 cut(s) 148, 930, 1188, 1338, 1531, 1677
BveI ACCTGC 3 cut(s) 21, 1697, 2447
Cac8I GCNNGC 6 cut(s) 496, 1142, 1818, 2610, 3125, 3736
CaiI CAGNNNCTG 1 cut(s) 929
CciI TCATGA 1 cut(s) 2062
CfoI GCGC 6 cut(s) 31, 841, 1323, 1800, 2626, 3433
Cfr10I RCCGGY 1 cut(s) 2939
Cfr13I GGNCC 4 cut(s) 10, 177, 320, 800
CseI GACGC 1 cut(s) 3574
CsiI ACCWGGT 2 cut(s) 411, 2876
Csp6I GTAC 7 cut(s) 454, 1072, 1895, 2888, 3321, 3508, 3616
CspAI ACCGGT 1 cut(s) 2939
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
CviQI GTAC 7 cut(s) 454, 1072, 1895, 2888, 3321, 3508, 3616
DraI TTTAAA 2 cut(s) 780, 3019
DraIII CACNNNGTG 2 cut(s) 725, 3551
EaeI YGGCCR 1 cut(s) 3802
Eam1104I CTCTTC 5 cut(s) 822, 1258, 1770, 1914, 3412
EarI CTCTTC 5 cut(s) 822, 1258, 1770, 1914, 3412
Ecl136II GAGCTC 1 cut(s) 2603
Eco130I CCWWGG 4 cut(s) 173, 186, 2019, 2792
Eco24I GRGCYC 3 cut(s) 2334, 2605, 3407
Eco31I GGTCTC 1 cut(s) 3172
Eco47I GGWCC 4 cut(s) 10, 177, 320, 800
Eco47III AGCGCT 2 cut(s) 840, 2625
Eco53kI GAGCTC 1 cut(s) 2603
Eco57I CTGAAG 5 cut(s) 967, 1507, 2090, 3393, 3722
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 2603
EcoO109I RGGNCCY 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 1510
EcoRII CCWGG 6 cut(s) 411, 817, 1033, 1561, 2876, 2983
EcoT14I CCWWGG 4 cut(s) 173, 186, 2019, 2792
EcoT22I ATGCAT 1 cut(s) 3637
EcoT38I GRGCYC 3 cut(s) 2334, 2605, 3407
ErhI CCWWGG 4 cut(s) 173, 186, 2019, 2792
Esp3I CGTCTC 1 cut(s) 3605
FauNDI CATATG 3 cut(s) 907, 2820, 2929
FbaI TGATCA 3 cut(s) 624, 1939, 2059
FblI GTMKAC 1 cut(s) 3099
FriOI GRGCYC 3 cut(s) 2334, 2605, 3407
FspBI CTAG 7 cut(s) 66, 755, 804, 978, 1824, 2120, 2609
FspI TGCGCA 1 cut(s) 1322
GlaI GCGC 6 cut(s) 30, 840, 1322, 1799, 2625, 3432
GsaI CCCAGC 2 cut(s) 200, 1282
GsuI CTGGAG 2 cut(s) 136, 474
HaeII RGCGCY 3 cut(s) 842, 1801, 2627
HaeIII GGCC 4 cut(s) 822, 1103, 1816, 3804
HapII CCGG 4 cut(s) 421, 2940, 2946, 3489
HgaI GACGC 1 cut(s) 3574
HhaI GCGC 6 cut(s) 31, 841, 1323, 1800, 2626, 3433
Hin6I GCGC 6 cut(s) 29, 839, 1321, 1798, 2624, 3431
HinP1I GCGC 6 cut(s) 29, 839, 1321, 1798, 2624, 3431
HincII GTYRAC 6 cut(s) 1306, 2443, 2469, 3419, 3497, 3525
HindII GTYRAC 6 cut(s) 1306, 2443, 2469, 3419, 3497, 3525
HindIII AAGCTT 4 cut(s) 496, 2243, 2402, 2429
HpaI GTTAAC 2 cut(s) 2443, 3525
HpaII CCGG 4 cut(s) 421, 2940, 2946, 3489
HphI GGTGA 3 cut(s) 1017, 1757, 1886
Hpy99I CGWCG 1 cut(s) 3755
HpyAV CCTTC 6 cut(s) 239, 984, 2429, 3113, 3240, 3338
HpyCH4III ACNGT 7 cut(s) 143, 435, 925, 2758, 2969, 3187, 3507
HpyCH4IV ACGT 6 cut(s) 292, 386, 1891, 3582, 3614, 3675
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 206, 556, 1141, 1817, 1966, 2108, 2609
HpySE526I ACGT 6 cut(s) 292, 386, 1891, 3582, 3614, 3675
HspAI GCGC 6 cut(s) 29, 839, 1321, 1798, 2624, 3431
Kpn2I TCCGGA 1 cut(s) 2945
Ksp22I TGATCA 3 cut(s) 624, 1939, 2059
KspAI GTTAAC 2 cut(s) 2443, 3525
LguI GCTCTTC 2 cut(s) 1770, 3412
LmnI GCTCC 4 cut(s) 155, 976, 2314, 2600
Lsp1109I GCAGC 6 cut(s) 94, 1261, 1807, 2098, 2133, 2566
LweI GCATC 9 cut(s) 252, 922, 1261, 1453, 2033, 2583, 3447, 3519, 3644
MabI ACCWGGT 2 cut(s) 411, 2876
MaeI CTAG 7 cut(s) 66, 755, 804, 978, 1824, 2120, 2609
MaeII ACGT 6 cut(s) 292, 386, 1891, 3582, 3614, 3675
MaeIII GTNAC 7 cut(s) 143, 407, 484, 1009, 1186, 1430, 3181
MfeI CAATTG 4 cut(s) 867, 1943, 2165, 2781
MflI RGATCY 4 cut(s) 373, 1081, 1843, 3370
MhlI GDGCHC 3 cut(s) 2334, 2605, 3407
MlyI GAGTC 4 cut(s) 731, 1181, 1931, 2706
MmeI TCCRAC 4 cut(s) 502, 1155, 1240, 3054
Mph1103I ATGCAT 1 cut(s) 3637
MroI TCCGGA 1 cut(s) 2945
MroXI GAANNNNTTC 2 cut(s) 434, 3768
MslI CAYNNNNRTG 2 cut(s) 1779, 2823
MspA1I CMGCKG 2 cut(s) 1282, 1640
MspCI CTTAAG 1 cut(s) 2462
MspI CCGG 4 cut(s) 421, 2940, 2946, 3489
MspR9I CCNGG 7 cut(s) 413, 422, 819, 1035, 1563, 2878, 2985
MunI CAATTG 4 cut(s) 867, 1943, 2165, 2781
Mva1269I GAATGC 2 cut(s) 1001, 1939
MvaI CCWGG 6 cut(s) 413, 819, 1035, 1563, 2878, 2985
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 8 cut(s) 113, 206, 556, 1141, 1817, 1966, 2108, 2609
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 2 cut(s) 186, 2792
NdeI CATATG 3 cut(s) 907, 2820, 2929
NheI GCTAGC 1 cut(s) 2608
NlaIV GGNNCC 4 cut(s) 162, 375, 2944, 3515
NmeAIII GCCGAG 1 cut(s) 1792
NmuCI GTSAC 3 cut(s) 143, 407, 1186
NsbI TGCGCA 1 cut(s) 1322
NsiI ATGCAT 1 cut(s) 3637
NspI RCATGY 2 cut(s) 1990, 3073
PagI TCATGA 1 cut(s) 2062
PaqCI CACCTGC 1 cut(s) 1697
PciI ACATGT 1 cut(s) 3069
PciSI GCTCTTC 2 cut(s) 1770, 3412
PcsI WCGNNNNNNNCGW 1 cut(s) 1399
PctI GAATGC 2 cut(s) 1001, 1939
PdmI GAANNNNTTC 2 cut(s) 434, 3768
PfeI GAWTC 8 cut(s) 274, 297, 467, 1331, 2360, 3152, 3542, 3596
PflFI GACNNNGTC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 1441
PfoI TCCNGGA 2 cut(s) 1033, 2983
PinAI ACCGGT 1 cut(s) 2939
PleI GAGTC 4 cut(s) 731, 1180, 1930, 2705
PpsI GAGTC 4 cut(s) 731, 1180, 1930, 2705
PpuMI RGGWCCY 1 cut(s) 177
PscI ACATGT 1 cut(s) 3069
PshBI ATTAAT 2 cut(s) 641, 762
Psp124BI GAGCTC 1 cut(s) 2605
Psp1406I AACGTT 2 cut(s) 3582, 3675
Psp5II RGGWCCY 1 cut(s) 177
Psp6I CCWGG 6 cut(s) 411, 817, 1033, 1561, 2876, 2983
PspFI CCCAGC 2 cut(s) 196, 1278
PspGI CCWGG 6 cut(s) 411, 817, 1033, 1561, 2876, 2983
PspN4I GGNNCC 4 cut(s) 162, 375, 2944, 3515
PspPI GGNCC 4 cut(s) 10, 177, 320, 800
PspPPI RGGWCCY 1 cut(s) 177
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstI CTGCAG 2 cut(s) 2899, 3010
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 4 cut(s) 373, 1081, 1843, 3370
PsyI GACNNNGTC 1 cut(s) 181
PvuII CAGCTG 2 cut(s) 1282, 1640
RsaI GTAC 7 cut(s) 455, 1073, 1896, 2889, 3322, 3509, 3617
RsaNI GTAC 7 cut(s) 454, 1072, 1895, 2888, 3321, 3508, 3616
RseI CAYNNNNRTG 2 cut(s) 1779, 2823
SacI GAGCTC 1 cut(s) 2605
SapI GCTCTTC 2 cut(s) 1770, 3412
Sau96I GGNCC 4 cut(s) 10, 177, 320, 800
SchI GAGTC 4 cut(s) 731, 1181, 1931, 2706
ScrFI CCNGG 7 cut(s) 413, 422, 819, 1035, 1563, 2878, 2985
SduI GDGCHC 3 cut(s) 2334, 2605, 3407
SexAI ACCWGGT 2 cut(s) 411, 2876
SfaNI GCATC 9 cut(s) 252, 922, 1261, 1453, 2033, 2583, 3447, 3519, 3644
SfcI CTRYAG 3 cut(s) 2670, 2895, 3006
SinI GGWCC 4 cut(s) 10, 177, 320, 800
SmiMI CAYNNNNRTG 2 cut(s) 1779, 2823
SmlI CTYRAG 3 cut(s) 1097, 2462, 2587
SmoI CTYRAG 3 cut(s) 1097, 2462, 2587
SsiI CCGC 6 cut(s) 164, 550, 823, 1104, 3697, 3805
SspI AATATT 4 cut(s) 620, 733, 1131, 3024
SspMI CTAG 7 cut(s) 66, 755, 804, 978, 1824, 2120, 2609
SstI GAGCTC 1 cut(s) 2605
StyD4I CCNGG 7 cut(s) 411, 420, 817, 1033, 1561, 2876, 2983
StyI CCWWGG 4 cut(s) 173, 186, 2019, 2792
TaaI ACNGT 7 cut(s) 143, 435, 925, 2758, 2969, 3187, 3507
TaiI ACGT 6 cut(s) 295, 389, 1894, 3585, 3617, 3678
TaqI TCGA 7 cut(s) 481, 853, 1114, 2229, 2707, 3315, 3646
TatI WGTACW 3 cut(s) 453, 1071, 1894
TauI GCSGC 4 cut(s) 825, 1106, 3700, 3807
TfiI GAWTC 8 cut(s) 274, 297, 467, 1331, 2360, 3152, 3542, 3596
TscAI CASTG 6 cut(s) 148, 930, 1195, 1345, 1531, 1684
TseFI GTSAC 3 cut(s) 143, 407, 1186
TseI GCWGC 6 cut(s) 107, 1274, 1820, 2111, 2146, 2579
Tsp45I GTSAC 3 cut(s) 143, 407, 1186
TspGWI ACGGA 2 cut(s) 1598, 1854
TspRI CASTG 6 cut(s) 148, 930, 1195, 1345, 1531, 1684
Tth111I GACNNNGTC 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 1441
Vha464I CTTAAG 1 cut(s) 2462
VpaK11BI GGWCC 4 cut(s) 10, 177, 320, 800
VspI ATTAAT 2 cut(s) 641, 762
XapI RAATTY 7 cut(s) 1348, 1447, 1510, 1752, 2375, 3044, 3768
XceI RCATGY 2 cut(s) 1990, 3073
XmiI GTMKAC 1 cut(s) 3099
XmnI GAANNNNTTC 2 cut(s) 434, 3768
XspI CTAG 7 cut(s) 66, 755, 804, 978, 1824, 2120, 2609
Zsp2I ATGCAT 1 cut(s) 3637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.