RLG00000016654
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009582
Physical Location & Seq
Forward (+)
8604420 .. 8611813
7394 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016654

Sequence Viewer

Length: 3804 bp
ATGGATCTACACCCATCAATGGCGCAAAGTGTGAGAACGAGTAGGTCCTCTTTCAGCTCCAGCAATGGCAATGAGGATACTCCTGTGCACAGCTCTACCGCATTTTTGAATGGGGATGATTATGACAGCGATAGCTCCAGCGTGGCACCACCTACACCAAGGACGCTATCGATGGATATCCCAGCAGAACTTGCTGGTGCCATACCCTTGATTGATAGATTTCAGGTGGAGGGATTTTTGAGGTTAATGCAGAAGCAGATTCAGTCTGCTGGAAAACGTGGGTTCTTTTCTAAAAAATCTGTTGGTCCTCAACCTCGAGAAAAGTTCACTTTTGAGGACATGCTGTGCTTCCAAAAGGATCCTATACCTACATCCTTACTTAAAATCAATAGTGACCTGGTTAGCCGGGCAATGAAACTGTTCCAGACCATTCTGAAATACATGGGAATTGATTCATCTGACCGATTTACTCCACCAAGCTTAGATGAAAGGATTGAACTTGTTGGAAAGTTGTATAAGCAAACATTGAAGCGAACAGAGCTCCGTGATGAACTATTTGCACAGATTTCCAAACAAACAAGAAATAATCCTGATAAGCAATGCTTAATTAAAGCATGGGAATTGATGTATTTGTGTGCATCCTCCATGCCCCCTAATAAGGACATCGGTGGCTATCTGTCTGAGTATGTCCATAATGTTGCACATGGTGCGAATATTGACTGTGATGTTCGAGCCCTAGCATTAAATACTTTAAATGCTTTGAAGCGTTCTGTCAAGGCTGGACCAAGGCATACAATACCAGGCCGGGAGGAGATAGAAGCACTTTTAACTGGTCGAAAGCTTACAACAATAGTGTTTTTTTTGGATGAAACTTTCGAAGAAATCACATATGACATGGCAACAACAGTTGCTGATGCTGTTGAGGAACTTGCAGGGATAATTAAATTGTCAGCATTTTCTAGCTTTAGTCTGTTTGAATGCCACAAAGTTGTAACGGGCTCGAAATCACCTGACCCCGGAAATGAGGAGTTTATTGGCCTAGATGACAACAAATACATAGGGGATCTGTTGGCAGAATTTAAAGCAGCAAAAGATCGAAGTAAAGGAGAAATTTTGCATTGCAAGCTGACATTTAAAAAGAAGCTATTTCGGGAGTCAGATGAAGCTGTAGCAGATCCAATGTTTGTGCAGTTGTCCTATGTTCAATTGCAACATGATTACATCATGGGAAATTATCCTGTGGGAAGAGACGATGCTGCACAGCTTTCTGCATTGCAAATATTGGTTGATATTGGATTCGTTGGTACCCCTGAATCATGCAATGACTGGAATTCGCTTTTGGAACGCTTTCTACCTAGACAAATTGCAATCACACGCGCAAAACGGGAGTGGGAGTTGGATATTCTTTCCCGTTACCATTCAATGCAAAACTTGACAAAAGATGATGCAAGACAACAATTCTTGCGAATACTGAGAACACTTCCTTACGGGAATTCAGTATTCTTCAGCGTTCGCAAGATAGATGATCCTATTGGACTTCTGCCCGGGCGGATCATTTTGGGAATCAACAAAAGAGGGGTTCATTTTTTTCGTCCTGTTCCAAAGGAATATCTACATTCAGCAGAACTAAGAGACATAATGCAATTTGGTAGTAGCAATACTGCTGTTTTCTTCAAGATGAGAGTTGCAGGTGTTCTTCACATATTCCAATTTGAAACTAAGCAGGGTGAAGAAATTTGCGTTGCTCTTCAAACACATATAAATGATGTCATGTTGCGGCGCTACTCTAAAGCTCGGACCGCTTCTAGTGGTTCGACGAATGGGGATATTTCTAACAATTTGAAGCCCTCTGATGAAGTGCATGAAAAACGTGTACAAGATTTGTCAAAAGCTGTTGAAGAATCTCAGCGGAATGTTGATCAATTGCTGGAAGAATTGCGTGAAAAACAAAAACAAGAAGCGAAACTGCAAGATGATTTGGATAATTTAAAGCAATCATTGGCATCTGAGAAGCAAAACTTGTCAGAAGTTGCAGGCGACCACAATAGACTAAAGTCATTGTGTGATGACAAGGACAAGGCACTTCAGGCTGCACTGGCAGAGAAGAAGAGCTTGGAAGCACAGTTGGCAAAGCTGAGTAATCAAGCAGTGCAGAAAAATGAGAAGACAAATCTGGTTGGTGGAGGAAACAACCAGGTTTTGGATAAACTCAAAGATGAGATAAAGCTTCGTACTGAGGAGTTAAAAGAAAAAGAAGGAACTATAAGGAGACTAGCAGACGATAAATTACTACTGGAGCAAACCATTTCTGGCCTTGAAAAGAGTAAAGCTGATGAGATTGATTCTCTTGAGAAAACTTTTGAGCAAGAGCGCAAAGCATTAAAGCTTCGAGTTTTTGAGCTTGAAAAGAAGCTTCAAGGAGTTAATCAAGAATTAGCTGTTCTAAAGTCAACTCTTGCAAGCAGGAACTCTGAGATTGCAGCATTACAGAATAACCTAAAGGAATTGGACGAATTGAGAGAAATGAAAGAGGATATCGATAGAAAGAATGAGCAAACTGCGTCCCTCTTGAGGATGCAGGGGGCTCAACTAGCTGAGATGGAATCCCTTTACAAGGAGGAACAACTTCTAAGGAAGCGCTATTTCAACACCATAGAAGATATGAAAGGCAAGATCAGAGTTTATTGTAGATTAAGACCTATGAGTGAAAAAGAGATTGCTGAAAAACAAGGATGTGCAGTTTCAAGTATTGATGAGTTTACAGTTGAACATCCATGGAAAGATGATAAACAGAAACAGCATACATATGATCGTGTATTTGATGAGCATGCCACCCAAGAAGATGTGTTTGAAGATACAAGGTATTTGGTACAATCTGCCGTAGATGGATACAACGTGTGCATATTTGCTTATGGGCAAACAGGTTCTGGGAAGACATATACCATTTATGGTTCAGAAACTAAACCTGGACTTACTCCTAGAGCTACTGCAGAACTTTTTAAAATCTTGAAGCGAGATAGCAACAAGTTCTCATTTTCTTTGAAGGCATATATGGTGGAATTGTATCAAGATACACTCTTAGACCTTCTATTGCCCAAAAATGCAAAGCGTCCAAAATTAGATATCAAAAAGGATTCAAAGGGCATGGTATCAGTTGAAAATATAACAGTTCTATCAATTTCGACACATGAGGAACTGAAAAACGTCATTCAAAGAGGATCTGAACAACGGCATACGTCAGGCACTCAAATGAATCAAGAAAGTTCAAGATCTCACCTCATAGTGTCAGTTATTATTGAAAGTACCAATCTTCAGACGCAGTCTGTTGCAAGAGGAAAGCTAAGTTTTGTGGATCTTGCTGGTTCGGAAAGAATAAAGAAGTCAGGTTCTGCGGGCAGTCAACTCAAAGAAGCTCAAAGCATCAACAAGTCACTTTCAGCACTCGGAGATGTGATTGGTGCTTTATCTTCTGGTGGACAGCACATACCCTACCGGAATCACAAACTAACAATGTTGATGAGTGATTCCCTTGGTGGTAATGCCAAGACGCTTATGTTTGTCAATTGTTCTCCTGCCGAATCAAACATGGATGAGACATACAATTCTCTAATGTATGCATCAAGAGTTCGAGCAATTGTGAATGATCCAAGCAAGAATGTATCTTCAAAAGAGATCATACGACTGAAAAAGTTGGTTTCTTATTGGAAAGAACAAGCTGGAAAGAGAGGAGAAGATGAGGATTTAGAAGAAATCCAGGACGAGCGGCCACCAAGAGAGAAAGGAGATGGCCGGCACTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

1268

Amino Acids

143.01

Weight (kDa)

6.62

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 170 - 274 7.8e-26 MyTH4 domain
RA_2 PF21989 280 - 380 3.1e-06 RA like domain
FERM_M PF00373 386 - 502 4.6e-22 FERM central domain
IRS PF02174 512 - 588 8.2e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 872 - 1030 2.9e-48 Microtubule binding
Kinesin PF00225 897 - 1212 1.2e-109 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1679
Acc36I ACCTGC 1 cut(s) 1679
Acc65I GGTACC 1 cut(s) 1304
AccB1I GGYRCC 3 cut(s) 145, 197, 1304
AccB7I CCANNNNNTGG 1 cut(s) 2200
AccBSI CCGCTC 1 cut(s) 3763
AccII CGCG 1 cut(s) 1377
AciI CCGC 7 cut(s) 99, 1549, 1777, 1800, 1909, 3392, 3763
AcoI YGGCCR 2 cut(s) 3764, 3787
AcsI RAATTY 5 cut(s) 1076, 1110, 1330, 1492, 1734
AcuI CTGAAG 3 cut(s) 1489, 2069, 3296
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 5 cut(s) 1306, 1875, 2233, 2871, 3304
AfeI AGCGCT 1 cut(s) 2639
AfiI CCNNNNNNNGG 6 cut(s) 19, 658, 1016, 2200, 2571, 2614
AflIII ACRYGT 2 cut(s) 1870, 2895
AjnI CCWGG 5 cut(s) 396, 799, 2193, 2965, 3753
AjuI GAANNNNNNNTTGG 4 cut(s) 1322, 1354, 2096, 2128
Alw21I GWGCWC 2 cut(s) 90, 543
Alw26I GTCTC 4 cut(s) 1242, 1626, 2263, 3587
Alw44I GTGCAC 1 cut(s) 86
AlwNI CAGNNNCTG 3 cut(s) 911, 2927, 3389
Ama87I CYCGRG 2 cut(s) 315, 1544
Aor51HI AGCGCT 1 cut(s) 2639
AoxI GGCC 5 cut(s) 802, 1036, 2311, 3764, 3787
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 4 cut(s) 1085, 1256, 2090, 2480
ApoI RAATTY 5 cut(s) 1076, 1110, 1330, 1492, 1734
Asp700I GAANNNNTTC 4 cut(s) 419, 451, 1347, 2625
Asp718I GGTACC 1 cut(s) 1304
AspLEI GCGC 5 cut(s) 25, 1379, 1782, 2373, 2640
AspS9I GGNCC 4 cut(s) 45, 305, 782, 1797
AsuC2I CCSGG 5 cut(s) 407, 806, 1017, 1545, 1546
AsuHPI GGTGA 3 cut(s) 999, 1739, 3266
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 2 cut(s) 315, 1544
AvaII GGWCC 4 cut(s) 45, 305, 782, 1797
BaeGI GKGCMC 1 cut(s) 90
BaeI ACNNNNGTAYC 2 cut(s) 69, 102
BamHI GGATCC 1 cut(s) 358
BanI GGYRCC 3 cut(s) 145, 197, 1304
BanII GRGCYC 4 cut(s) 543, 736, 1001, 2587
BbsI GAAGAC 2 cut(s) 2171, 2939
Bbv12I GWGCWC 2 cut(s) 90, 543
BbvI GCAGC 4 cut(s) 1097, 1243, 2077, 2492
BccI CCATC 5 cut(s) 22, 166, 2593, 2879, 3779
BceAI ACGGC 2 cut(s) 2864, 3245
BciT130I CCWGG 5 cut(s) 398, 801, 2195, 2967, 3755
BciVI GTATCC 2 cut(s) 70, 2882
BclI TGATCA 1 cut(s) 1918
BcnI CCSGG 5 cut(s) 407, 806, 1017, 1545, 1546
BcoDI GTCTC 4 cut(s) 1242, 1626, 2263, 3587
BfaI CTAG 8 cut(s) 737, 960, 1040, 1356, 1806, 2273, 2591, 2979
BfmI CTRYAG 2 cut(s) 1167, 2988
BfoI RGCGCY 2 cut(s) 1783, 2641
BfuAI ACCTGC 1 cut(s) 1679
BfuI GTATCC 2 cut(s) 70, 2882
BglII AGATCT 1 cut(s) 3269
BisI GCNGC 6 cut(s) 1086, 1257, 1778, 2091, 2481, 3764
BlsI GCNGC 6 cut(s) 1087, 1258, 1779, 2092, 2482, 3765
Bme18I GGWCC 4 cut(s) 45, 305, 782, 1797
BmeT110I CYCGRG 2 cut(s) 315, 1544
BmgT120I GGNCC 4 cut(s) 45, 305, 782, 1797
BmiI GGNNCC 4 cut(s) 147, 199, 360, 1306
BmsI GCATC 8 cut(s) 647, 904, 1243, 1435, 2012, 2565, 3429, 3626
BoxI GACNNNNGTC 1 cut(s) 2053
BpiI GAAGAC 2 cut(s) 2171, 2939
BpmI CTGGAG 3 cut(s) 43, 121, 2315
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 2369, 2590
BpuMI CCSGG 5 cut(s) 407, 806, 1017, 1545, 1546
Bsa29I ATCGAT 2 cut(s) 170, 2538
BsaBI GATNNNNATC 1 cut(s) 176
BsaJI CCNNGG 6 cut(s) 158, 785, 1015, 1544, 2774, 3529
BsaWI WCCGGW 1 cut(s) 3492
BsaXI ACNNNNNCTCC 2 cut(s) 1379, 1409
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 658, 1016, 2200, 2571, 2614
Bse118I RCCGGY 1 cut(s) 3789
Bse1I ACTGG 4 cut(s) 835, 1331, 2100, 2298
Bse3DI GCAATG 7 cut(s) 70, 76, 417, 605, 1117, 1271, 1327
Bse8I GATNNNNATC 1 cut(s) 176
BseBI CCWGG 5 cut(s) 398, 801, 2195, 2967, 3755
BseCI ATCGAT 2 cut(s) 170, 2538
BseDI CCNNGG 6 cut(s) 158, 785, 1015, 1544, 2774, 3529
BseGI GGATG 8 cut(s) 121, 371, 638, 871, 2580, 2738, 2770, 3595
BseJI GATNNNNATC 1 cut(s) 176
BseLI CCNNNNNNNGG 6 cut(s) 19, 658, 1016, 2200, 2571, 2614
BseMI GCAATG 7 cut(s) 70, 76, 417, 605, 1117, 1271, 1327
BseMII CTCAG 8 cut(s) 672, 1463, 1919, 1998, 2126, 2226, 2463, 2586
BseNI ACTGG 4 cut(s) 835, 1331, 2100, 2298
BseRI GAGGAG 4 cut(s) 824, 1040, 2252, 3741
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 4 cut(s) 1097, 1243, 2077, 2492
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 5 cut(s) 1208, 1242, 2076, 2171, 2757
Bsh1236I CGCG 1 cut(s) 1377
BshFI GGCC 5 cut(s) 804, 1038, 2313, 3766, 3789
BshNI GGYRCC 3 cut(s) 145, 197, 1304
BshVI ATCGAT 2 cut(s) 170, 2538
BsiHKAI GWGCWC 2 cut(s) 90, 543
BsiHKCI CYCGRG 2 cut(s) 315, 1544
BsiSI CCGG 6 cut(s) 406, 805, 1017, 1545, 3493, 3790
BslFI GGGAC 1 cut(s) 2548
BslI CCNNNNNNNGG 6 cut(s) 19, 658, 1016, 2200, 2571, 2614
BsmAI GTCTC 4 cut(s) 1242, 1626, 2263, 3587
BsmBI CGTCTC 1 cut(s) 1242
BsmFI GGGAC 1 cut(s) 2548
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 5 cut(s) 804, 1038, 2313, 3766, 3789
BsoBI CYCGRG 2 cut(s) 315, 1544
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 5 cut(s) 90, 543, 736, 1001, 2587
Bsp1407I TGTACA 1 cut(s) 1873
Bsp19I CCATGG 1 cut(s) 2774
BspACI CCGC 7 cut(s) 99, 1549, 1777, 1800, 1909, 3392, 3763
BspANI GGCC 5 cut(s) 804, 1038, 2313, 3766, 3789
BspCNI CTCAG 8 cut(s) 673, 1464, 1918, 1999, 2127, 2227, 2464, 2587
BspDI ATCGAT 2 cut(s) 170, 2538
BspFNI CGCG 1 cut(s) 1377
BspLI GGNNCC 4 cut(s) 147, 199, 360, 1306
BspMAI CTGCAG 1 cut(s) 2992
BspMI ACCTGC 1 cut(s) 1679
BspQI GCTCTTC 2 cut(s) 1752, 2102
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 3 cut(s) 145, 197, 1304
BsrBI CCGCTC 1 cut(s) 3763
BsrDI GCAATG 7 cut(s) 70, 76, 417, 605, 1117, 1271, 1327
BsrFI RCCGGY 1 cut(s) 3789
BsrGI TGTACA 1 cut(s) 1873
BsrI ACTGG 4 cut(s) 835, 1331, 2100, 2298
BssAI RCCGGY 1 cut(s) 3789
BssECI CCNNGG 6 cut(s) 158, 785, 1015, 1544, 2774, 3529
BssT1I CCWWGG 4 cut(s) 158, 785, 2774, 3529
Bst2UI CCWGG 5 cut(s) 398, 801, 2195, 2967, 3755
Bst4CI ACNGT 6 cut(s) 420, 722, 907, 2124, 2764, 3169
Bst6I CTCTTC 3 cut(s) 1240, 1752, 2102
BstAPI GCANNNNNTGC 2 cut(s) 191, 707
BstAUI TGTACA 1 cut(s) 1873
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 6 cut(s) 1124, 2035, 2461, 2829, 3394, 3791
BstDSI CCRYGG 1 cut(s) 2774
BstENI CCTNNNNNAGG 1 cut(s) 2612
BstF5I GGATG 8 cut(s) 121, 371, 638, 871, 2580, 2738, 2770, 3595
BstFNI CGCG 1 cut(s) 1377
BstH2I RGCGCY 2 cut(s) 1783, 2641
BstHHI GCGC 5 cut(s) 25, 1379, 1782, 2373, 2640
BstMAI GTCTC 4 cut(s) 1242, 1626, 2263, 3587
BstMWI GCNNNNNNNGC 9 cut(s) 191, 538, 707, 1123, 1799, 2087, 2096, 2126, 2591
BstNI CCWGG 5 cut(s) 398, 801, 2195, 2967, 3755
BstNSI RCATGY 2 cut(s) 343, 2831
BstPAI GACNNNNGTC 1 cut(s) 2053
BstSFI CTRYAG 2 cut(s) 1167, 2988
BstSLI GKGCMC 1 cut(s) 90
BstUI CGCG 1 cut(s) 1377
BstV1I GCAGC 4 cut(s) 1097, 1243, 2077, 2492
BstV2I GAAGAC 2 cut(s) 2171, 2939
BstX2I RGATCY 7 cut(s) 4, 358, 1063, 1174, 3218, 3269, 3352
BstYI RGATCY 7 cut(s) 4, 358, 1063, 1174, 3218, 3269, 3352
Bsu15I ATCGAT 2 cut(s) 170, 2538
BsuI GTATCC 2 cut(s) 70, 2882
BsuRI GGCC 5 cut(s) 804, 1038, 2313, 3766, 3789
BsuTUI ATCGAT 2 cut(s) 170, 2538
BtgI CCRYGG 1 cut(s) 2774
BtsCI GGATG 8 cut(s) 121, 371, 638, 871, 2580, 2738, 2770, 3595
BtsI GCAGTG 1 cut(s) 2154
BtsIMutI CAGTG 2 cut(s) 2093, 2154
BveI ACCTGC 1 cut(s) 1679
Cac8I GCNNGC 6 cut(s) 1124, 2035, 2461, 2829, 3394, 3791
CaiI CAGNNNCTG 3 cut(s) 911, 2927, 3389
CfoI GCGC 5 cut(s) 25, 1379, 1782, 2373, 2640
Cfr10I RCCGGY 1 cut(s) 3789
Cfr13I GGNCC 4 cut(s) 45, 305, 782, 1797
Cfr9I CCCGGG 1 cut(s) 1544
ClaI ATCGAT 2 cut(s) 170, 2538
CpoI CGGWCCG 1 cut(s) 1797
CseI GACGC 5 cut(s) 172, 2550, 3098, 3325, 3556
CsiI ACCWGGT 2 cut(s) 396, 2193
Csp6I GTAC 5 cut(s) 1305, 1874, 2232, 2870, 3303
CspI CGGWCCG 1 cut(s) 1797
CviQI GTAC 5 cut(s) 1305, 1874, 2232, 2870, 3303
DraI TTTAAA 5 cut(s) 753, 1081, 1135, 1989, 3001
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 2 cut(s) 3764, 3787
Eam1104I CTCTTC 3 cut(s) 1240, 1752, 2102
EarI CTCTTC 3 cut(s) 1240, 1752, 2102
EciI GGCGGA 1 cut(s) 1564
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 4 cut(s) 158, 785, 2774, 3529
Eco24I GRGCYC 4 cut(s) 543, 736, 1001, 2587
Eco32I GATATC 3 cut(s) 178, 2536, 3124
Eco47I GGWCC 4 cut(s) 45, 305, 782, 1797
Eco47III AGCGCT 1 cut(s) 2639
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 3 cut(s) 1489, 2069, 3296
Eco88I CYCGRG 2 cut(s) 315, 1544
EcoICRI GAGCTC 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 2612
EcoO109I RGGNCCY 1 cut(s) 45
EcoRI GAATTC 2 cut(s) 1330, 1492
EcoRII CCWGG 5 cut(s) 396, 799, 2193, 2965, 3753
EcoRV GATATC 3 cut(s) 178, 2536, 3124
EcoT14I CCWWGG 4 cut(s) 158, 785, 2774, 3529
EcoT22I ATGCAT 1 cut(s) 3619
EcoT38I GRGCYC 4 cut(s) 543, 736, 1001, 2587
ErhI CCWWGG 4 cut(s) 158, 785, 2774, 3529
Esp3I CGTCTC 1 cut(s) 1242
FalI AAGNNNNNCTT 6 cut(s) 587, 619, 2003, 2035, 2096, 2128
FaqI GGGAC 1 cut(s) 2548
FauI CCCGC 1 cut(s) 3385
FauNDI CATATG 2 cut(s) 889, 2806
FbaI TGATCA 1 cut(s) 1918
Fnu4HI GCNGC 6 cut(s) 1086, 1257, 1778, 2091, 2481, 3764
FokI GGATG 8 cut(s) 128, 358, 625, 878, 2587, 2745, 2757, 3602
FriOI GRGCYC 4 cut(s) 543, 736, 1001, 2587
Fsp4HI GCNGC 6 cut(s) 1086, 1257, 1778, 2091, 2481, 3764
FspBI CTAG 8 cut(s) 737, 960, 1040, 1356, 1806, 2273, 2591, 2979
GlaI GCGC 5 cut(s) 24, 1378, 1781, 2372, 2639
GluI GCNGC 6 cut(s) 1086, 1257, 1778, 2091, 2481, 3764
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 3 cut(s) 43, 121, 2315
HaeII RGCGCY 2 cut(s) 1783, 2641
HaeIII GGCC 5 cut(s) 804, 1038, 2313, 3766, 3789
HapII CCGG 6 cut(s) 406, 805, 1017, 1545, 3493, 3790
HgaI GACGC 5 cut(s) 172, 2550, 3098, 3325, 3556
HhaI GCGC 5 cut(s) 25, 1379, 1782, 2373, 2640
Hin6I GCGC 5 cut(s) 23, 1377, 1780, 2371, 2638
HinP1I GCGC 5 cut(s) 23, 1377, 1780, 2371, 2638
HincII GTYRAC 2 cut(s) 2451, 3401
HindII GTYRAC 2 cut(s) 2451, 3401
HindIII AAGCTT 5 cut(s) 478, 839, 2225, 2384, 2411
HpaII CCGG 6 cut(s) 406, 805, 1017, 1545, 3493, 3790
HphI GGTGA 3 cut(s) 999, 1739, 3266
Hpy166II GTNNAC 7 cut(s) 88, 327, 1874, 2451, 2760, 3401, 3476
Hpy8I GTNNAC 7 cut(s) 88, 327, 1874, 2451, 2760, 3401, 3476
Hpy99I CGWCG 1 cut(s) 1819
HpyAV CCTTC 3 cut(s) 2249, 3037, 3095
HpyCH4III ACNGT 6 cut(s) 420, 722, 907, 2124, 2764, 3169
HpyCH4IV ACGT 5 cut(s) 277, 1870, 2895, 3204, 3236
HpyF10VI GCNNNNNNNGC 9 cut(s) 191, 538, 707, 1123, 1799, 2087, 2096, 2126, 2591
HpySE526I ACGT 5 cut(s) 277, 1870, 2895, 3204, 3236
HspAI GCGC 5 cut(s) 23, 1377, 1780, 2371, 2638
KpnI GGTACC 1 cut(s) 1308
KroI GCCGGC 1 cut(s) 3789
KroNI GCCGGC 1 cut(s) 3791
Ksp22I TGATCA 1 cut(s) 1918
LguI GCTCTTC 2 cut(s) 1752, 2102
LmnI GCTCC 4 cut(s) 62, 140, 546, 2296
Lsp1109I GCAGC 4 cut(s) 1097, 1243, 2077, 2492
LweI GCATC 8 cut(s) 647, 904, 1243, 1435, 2012, 2565, 3429, 3626
MabI ACCWGGT 2 cut(s) 396, 2193
MaeI CTAG 8 cut(s) 737, 960, 1040, 1356, 1806, 2273, 2591, 2979
MaeII ACGT 5 cut(s) 277, 1870, 2895, 3204, 3236
MaeIII GTNAC 4 cut(s) 392, 991, 1412, 3429
MbiI CCGCTC 1 cut(s) 3763
MfeI CAATTG 4 cut(s) 1205, 1922, 3562, 3633
MflI RGATCY 7 cut(s) 4, 358, 1063, 1174, 3218, 3269, 3352
MhlI GDGCHC 5 cut(s) 90, 543, 736, 1001, 2587
MlyI GAGTC 1 cut(s) 1163
MmeI TCCRAC 2 cut(s) 484, 1377
Mph1103I ATGCAT 1 cut(s) 3619
MroNI GCCGGC 1 cut(s) 3789
MroXI GAANNNNTTC 4 cut(s) 419, 451, 1347, 2625
MslI CAYNNNNRTG 3 cut(s) 1761, 2805, 3248
MspA1I CMGCKG 1 cut(s) 1909
MspI CCGG 6 cut(s) 406, 805, 1017, 1545, 3493, 3790
MunI CAATTG 4 cut(s) 1205, 1922, 3562, 3633
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 5 cut(s) 398, 801, 2195, 2967, 3755
MvnI CGCG 1 cut(s) 1377
MwoI GCNNNNNNNGC 9 cut(s) 191, 538, 707, 1123, 1799, 2087, 2096, 2126, 2591
NaeI GCCGGC 1 cut(s) 3791
NciI CCSGG 5 cut(s) 407, 806, 1017, 1545, 1546
NcoI CCATGG 1 cut(s) 2774
NdeI CATATG 2 cut(s) 889, 2806
NgoMIV GCCGGC 1 cut(s) 3789
NlaIV GGNNCC 4 cut(s) 147, 199, 360, 1306
NmuCI GTSAC 2 cut(s) 392, 3429
NsiI ATGCAT 1 cut(s) 3619
NspI RCATGY 2 cut(s) 343, 2831
NspV TTCGAA 1 cut(s) 876
PacI TTAATTAA 1 cut(s) 609
PaeI GCATGC 1 cut(s) 2831
PaeR7I CTCGAG 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 1679
PciSI GCTCTTC 2 cut(s) 1752, 2102
PctI GAATGC 1 cut(s) 983
PdiI GCCGGC 1 cut(s) 3791
PdmI GAANNNNTTC 4 cut(s) 419, 451, 1347, 2625
PflFI GACNNNGTC 1 cut(s) 3319
PflMI CCANNNNNTGG 1 cut(s) 2200
PfoI TCCNGGA 1 cut(s) 3753
PkrI GCNGC 6 cut(s) 1087, 1258, 1779, 2092, 2482, 3765
PleI GAGTC 1 cut(s) 1162
PpsI GAGTC 1 cut(s) 1162
PpuMI RGGWCCY 1 cut(s) 45
PshAI GACNNNNGTC 1 cut(s) 2053
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 45
Psp6I CCWGG 5 cut(s) 396, 799, 2193, 2965, 3753
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 5 cut(s) 396, 799, 2193, 2965, 3753
PspN4I GGNNCC 4 cut(s) 147, 199, 360, 1306
PspPI GGNCC 4 cut(s) 45, 305, 782, 1797
PspPPI RGGWCCY 1 cut(s) 45
PsrI GAACNNNNNNTAC 2 cut(s) 1335, 1367
PstI CTGCAG 1 cut(s) 2992
PstNI CAGNNNCTG 3 cut(s) 911, 2927, 3389
PsuI RGATCY 7 cut(s) 4, 358, 1063, 1174, 3218, 3269, 3352
PsyI GACNNNGTC 1 cut(s) 3319
RsaI GTAC 5 cut(s) 1306, 1875, 2233, 2871, 3304
RsaNI GTAC 5 cut(s) 1305, 1874, 2232, 2870, 3303
RseI CAYNNNNRTG 3 cut(s) 1761, 2805, 3248
Rsr2I CGGWCCG 1 cut(s) 1797
RsrII CGGWCCG 1 cut(s) 1797
SacI GAGCTC 1 cut(s) 543
SapI GCTCTTC 2 cut(s) 1752, 2102
SatI GCNGC 6 cut(s) 1086, 1257, 1778, 2091, 2481, 3764
Sau96I GGNCC 4 cut(s) 45, 305, 782, 1797
SchI GAGTC 1 cut(s) 1163
SduI GDGCHC 5 cut(s) 90, 543, 736, 1001, 2587
SexAI ACCWGGT 2 cut(s) 396, 2193
SfaNI GCATC 8 cut(s) 647, 904, 1243, 1435, 2012, 2565, 3429, 3626
SfcI CTRYAG 2 cut(s) 1167, 2988
Sfr274I CTCGAG 1 cut(s) 315
SfuI TTCGAA 1 cut(s) 876
SinI GGWCC 4 cut(s) 45, 305, 782, 1797
SlaI CTCGAG 1 cut(s) 315
SmaI CCCGGG 1 cut(s) 1546
SmiMI CAYNNNNRTG 3 cut(s) 1761, 2805, 3248
SmlI CTYRAG 3 cut(s) 315, 2348, 2569
SmoI CTYRAG 3 cut(s) 315, 2348, 2569
SphI GCATGC 1 cut(s) 2831
SrfI GCCCGGGC 1 cut(s) 1546
SsiI CCGC 7 cut(s) 99, 1549, 1777, 1800, 1909, 3392, 3763
SspI AATATT 2 cut(s) 715, 1281
SspMI CTAG 8 cut(s) 737, 960, 1040, 1356, 1806, 2273, 2591, 2979
SstI GAGCTC 1 cut(s) 543
StyI CCWWGG 4 cut(s) 158, 785, 2774, 3529
TaaI ACNGT 6 cut(s) 420, 722, 907, 2124, 2764, 3169
TaiI ACGT 5 cut(s) 280, 1873, 2898, 3207, 3239
TaqII GACCGA 1 cut(s) 477
TatI WGTACW 1 cut(s) 1873
TauI GCSGC 2 cut(s) 1780, 3766
TscAI CASTG 2 cut(s) 2100, 2154
TseFI GTSAC 2 cut(s) 392, 3429
TseI GCWGC 4 cut(s) 1085, 1256, 2090, 2480
Tsp45I GTSAC 2 cut(s) 392, 3429
TspGWI ACGGA 1 cut(s) 533
TspMI CCCGGG 1 cut(s) 1544
TspRI CASTG 2 cut(s) 2100, 2154
Tth111I GACNNNGTC 1 cut(s) 3319
Van91I CCANNNNNTGG 1 cut(s) 2200
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 4 cut(s) 45, 305, 782, 1797
XagI CCTNNNNNAGG 1 cut(s) 2612
XapI RAATTY 5 cut(s) 1076, 1110, 1330, 1492, 1734
XceI RCATGY 2 cut(s) 343, 2831
XhoI CTCGAG 1 cut(s) 315
XmaI CCCGGG 1 cut(s) 1544
XmnI GAANNNNTTC 4 cut(s) 419, 451, 1347, 2625
XspI CTAG 8 cut(s) 737, 960, 1040, 1356, 1806, 2273, 2591, 2979
Zsp2I ATGCAT 1 cut(s) 3619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.