Rh2CG110300
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009582
Physical Location & Seq
Forward (+)
9726735 .. 9734118
7384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG110300.1

Sequence Viewer

Length: 3810 bp
ATGACAATGGATCTACACCCATCAATGGCGCAAAGTGTGAGAACGAGTAGGTCCTCTTTTAGCTCCAGCAATGGCAATGAGGATACTCCTGTGCACAGCTCTACCGCATTTTTGAATGGGGATGATTATGACAGCGATAGCTCCAGCGTGGCACCACCTACACCAAGGACGCTATCGATGGATATCCCAGCAGAACTTGCTGGTGCCATACCCTTGATTGATAGATTTCAGGTAGAGGGATTTTTGAGGTTAATGCAGAAGCAGATTCAGTCTGCTGGAAAACGGGGGTTCTTTTCTAAAAAATCGGTTGGTCCTCAACCTCGAGAAAAGTTCACTTTTGAGGACATGCTGTGCTTCCAAAAGGATCCTATACCTACATCCTTACTTAAAATCAATAGTGACCTGGTTAGCCGGGCAATGAAACTGTTCCAGACCATTCTGAAATACATGGGAATCGACTCATCTGACCGAATTACTCCACCAAGCTTAGATGAAAGGATTGAACTTGTTGGAAAGTTGTATAAGCAAACATTGAAGCGAACAGAGCTCCGTGATGAGCTTTTTGCACAGATTTCCAAACAAACAAGAAATAATCCTGATAAGCAATGCTTAATTAAAGCATGGGAATTGATGTATTTGTGTGCATCCTCCATGCCCCCTAATAAGGACATCGGTGGCTATCTGTCTGAGTATGTCCATAATGTTGCACATGGTGCGAATATTGACTGTGATGTTCGAGTCCTAGCATTAAATACTTTAAATGCTTTGAAGCGTTCTGTCAAGGCTGGACCAAGGCATACAATACCAGGCCGGGAGGAGATAGAAGCACTTTTAACTGGTCGAAAGCTTACAACAATAGTGTTTTTTTTGGATGAAACTTTCGAAGAAATCACATATGACATGGCAACAACAGTGGCTGATGCTGTTGAGGAACTTGCTGGGATAATTAAATTGTCAGCATTTTCTAGCTTTAGTCTGTTTGAATGCCACAAAGTTGTAACGGGCTCGAAATCACCTGACCTCGGAAATGAGGAGTATATTGGCCTAGATGACAACAAATACATAGGGGATCTGTTGGCAGAATTTAAAGCAGCAAAAGATCGAAGTAAAGGAGAAATTTTGCACTGCAAGCTGACATTTAAAAAGAAGCTATTTCGGGAGTCAGATGAAGCTGTAGCAGATCCAATGTTTGTGCAGTTGTCCTATGTTCAATTGCAACATGATTACATCATGGGAAATTACCCTGTGGGAAGAGACGATGCTGCACAGCTTTCTGCATTGCAAATATTGGTTGATATTGGATTCGTTGGTACCCCTGAATCATGCAATGACTGGAATTCGCTTTTGGAACGCTTTCTACCTAGACAAATTGCAATCACTCGCGCAAAACGGGAGTGGGAGTTGGATATTCTTTCTCGTTACCATTCAATGCAAAACTTGACAAAAGATGATGCAAGACAACAATTCTTGCGAATACTGAGAACACTTCCTTATGGGAATTCAGTGTTCTTCAGTGTTCGCAAGATAGATGATCCTATTGGACTTCTGCCCGGGCGGATCATTTTGGGAATCAACAAAAGAGGGGTTCATTTTTTTCGTCCCGTTCCAAAGGAATATCTACATTCAGCAGAACTAAGAGACATAATGCAATTTGGTAGTAGCAATACTGCTGTTTTCTTCAAGATGAGAGTTGCAGGTGTTCTTCACATATTCCAATTTGAAACTAAGCAGGGTGAAGAAATTTGCGTTGCTCTTCAAACACATATAAATGATGTCATGTTGCGCCGCTACTCTAAAGCTCGGACCGCTTCTAGTGGTTCGACGAATGGAGATATTTCCAACAATTTGAAGCCCTCTGATGAAGTGCATGAAAAACTTGTACAAGATTTGTCAAAAGCTGTTGAAGAATCTCAGCGGAATGTTGATCAATTGCTGGAAGAATTGCGTGAAAAACAAAAACAAGAAGCAAAACTGCAAGATGATTTGGATAATTTAAAGCAATCATTGGCATCTGAGAAGCAAAACTTGTCAGAAGTTGCAGGCGACCACAATAGACTAAAGTCATTGTGTGATGACAAGGACAGGGCACTTAAGGCTGCACTGGCAGAGAAGAAGAGCTTGGAAGCACAGTTGGCAAAGCTGAGTAATCAAGCAGTGCAGAAAAATGACAAGACAAATCTGGTTGGTGGAGGAAACAACCAGGTTTTGGATAAACTCAAAGATGAGATAAAGCTTCGTACTGAGGAGTTAAAAGAAAAAGAAGAAACTATAAGGAGACTAGCGGACGATAAATTACTACTGGAGCAAACCATATCTGGCCTTGAAAAGAGTAAAGCTGATGAGATTGATTCTCATGAGAAAACTTTTGAGCAAGAGCGCAAAGCATTAAAGCTTCGAGTTTTTGAACTTGAAAAGAAGCTTGAAGGAGTTAATCAAGAATTAGCTGTTCTAAAGTCAACTCTTGCAAGCAGGAACTCTGAGATTGCTGCATTACAGAATAACCTAAAGGAATTGGACGAATTAAGAGAAATGAAAGAGGATATCGATAGAAAGAATGAGCAAACTGCCTCCCTCTTGAGGATGCAGGGGGCTCAACTAGCTGAGATGGAATCCCTTTACAAGGAGGAACAACTTCTAAGGAAGCGCTATTTCAACACCATAGAAGATATGAAAGGCAAGATCAGAGTTTATTGTAGATTAAGACCTATGAGTGAAAAAGAGATTGCTGAAAAACAAGGATGTGCAGTTTCAAGTATTGATGAGTTTACAGTCGAACATCCATGGAAAGATGAAAAACAGAAACAGCATACATATGATCGTGTATTTGATGAGCATGCCACCCAAGAAGATGTGTTTGAAGATACAAAGTATTTGGTACAATCTGCCGTAGATGGATACAACGTGTGCATATTTGCTTATGGGCAAACAGGTTCTGGGAAGACATATACCATTTATGGTTCAGAAACTAAACCTGGACTTACTCCTAGAGCTACTGCAGAACTTTTTAAAATCTTGAAGCGAGATAGCAACAAGTTCTCATTTTCTTTGAAGGCATATATGGTGGAATTGTATCAAGATACACTGTTAGACCTTCTATTGCCCAAAAATGCAAAGCGTCCAAAATTAGATATCAAAAAGGATTCAAAGGGCATGGTATCAGTTGAAAATATAACGGTTCTATCAATTTCGACACATGAGGAGCTGAAAAACGTCATTCAAAGAGGATCGGAACGACGGCATACGTCAGGCACTCAAATGAATCAAGAAAGTTCAAGATCTCACCTCATAGTGTCAGTTATTATTGAAAGTACCAATCTTCAGACACAGTCTGTTGCAAGAGGAAAGCTAAGTTTTGTGGATCTTGCTGGTTCGGAAAGAATAAAGAAGTCAGGTTCTGCGGGCAGTCAACTCAAAGAAGCTCAAAGCATCAACAAGTCACTTTCAGCACTCGGAGATGTGATTGGTGCTTTATCTTCTGGTGGACAGCACATACCCTACCGGAATCACAAACTAACAATGTTGATGAGTGATTCCCTTGGTGGTAATGCCAAGACGCTTATGTTTGTCAATTGTTCTCCTGCCGAATCAAACATGGATGAGACATATAATTCTCTAATGTATGCATCAAGAGTTCGAGCAATCGTGAATGATCCAAGCAAGAATGTATCTTCAAAAGAGATCATGCGACTGAAAAAGTTGGTTTCTTATTGGAAAGAACAAGCTGGAAAGAGAGGAGAAGATGAGGATTTAGAAGAAATCCAGGACGAGCGGCCACCAAGAGAGAAAGGAGATGGCCGGCACTCGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

1269

Amino Acids

143.37

Weight (kDa)

6.55

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 172 - 276 1.1e-25 MyTH4 domain
RA_2 PF21989 282 - 383 2.1e-06 RA like domain
FERM_M PF00373 388 - 504 4.6e-22 FERM central domain
IRS PF02174 514 - 590 8.3e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 874 - 1032 2.2e-48 Microtubule binding
Kinesin PF00225 899 - 1214 9.7e-110 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1685
Acc36I ACCTGC 1 cut(s) 1685
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 3 cut(s) 151, 203, 1310
AccB7I CCANNNNNTGG 1 cut(s) 2206
AccBSI CCGCTC 1 cut(s) 3769
AccII CGCG 1 cut(s) 1383
AciI CCGC 8 cut(s) 105, 1555, 1786, 1806, 1915, 2282, 3398, 3769
AcoI YGGCCR 2 cut(s) 3770, 3793
AcsI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
AcuI CTGAAG 2 cut(s) 1495, 3302
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 5 cut(s) 1312, 1881, 2239, 2877, 3310
AfeI AGCGCT 1 cut(s) 2645
AfiI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 1 cut(s) 2901
AjnI CCWGG 5 cut(s) 402, 805, 2199, 2971, 3759
AjuI GAANNNNNNNTTGG 4 cut(s) 1328, 1360, 2102, 2134
Alw21I GWGCWC 2 cut(s) 96, 549
Alw26I GTCTC 4 cut(s) 1248, 1632, 2269, 3593
Alw44I GTGCAC 1 cut(s) 92
AlwNI CAGNNNCTG 3 cut(s) 917, 2933, 3395
Ama87I CYCGRG 2 cut(s) 321, 1550
Aor51HI AGCGCT 1 cut(s) 2645
AoxI GGCC 5 cut(s) 808, 1042, 2317, 3770, 3793
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 1091, 1262, 2096, 2486
ApoI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
Asp700I GAANNNNTTC 3 cut(s) 425, 1353, 2631
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
AspS9I GGNCC 4 cut(s) 51, 311, 788, 1803
AsuC2I CCSGG 4 cut(s) 413, 812, 1551, 1552
AsuHPI GGTGA 3 cut(s) 1005, 1745, 3272
AsuII TTCGAA 1 cut(s) 882
AvaI CYCGRG 2 cut(s) 321, 1550
AvaII GGWCC 4 cut(s) 51, 311, 788, 1803
BaeGI GKGCMC 2 cut(s) 96, 2089
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 3 cut(s) 151, 203, 1310
BanII GRGCYC 3 cut(s) 549, 1007, 2593
BbsI GAAGAC 1 cut(s) 2945
Bbv12I GWGCWC 2 cut(s) 96, 549
BbvI GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BccI CCATC 5 cut(s) 28, 172, 2599, 2885, 3785
BceAI ACGGC 2 cut(s) 2870, 3251
BciT130I CCWGG 5 cut(s) 404, 807, 2201, 2973, 3761
BciVI GTATCC 2 cut(s) 76, 2888
BclI TGATCA 1 cut(s) 1924
BcnI CCSGG 4 cut(s) 413, 812, 1551, 1552
BcoDI GTCTC 4 cut(s) 1248, 1632, 2269, 3593
BfaI CTAG 8 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597, 2985
BfmI CTRYAG 2 cut(s) 1173, 2994
BfoI RGCGCY 1 cut(s) 2647
BfrI CTTAAG 1 cut(s) 2090
BfuAI ACCTGC 1 cut(s) 1685
BfuI GTATCC 2 cut(s) 76, 2888
BglII AGATCT 1 cut(s) 3275
BisI GCNGC 6 cut(s) 1092, 1263, 1786, 2097, 2487, 3770
BlsI GCNGC 6 cut(s) 1093, 1264, 1787, 2098, 2488, 3771
Bme1390I CCNGG 9 cut(s) 404, 413, 807, 812, 1551, 1552, 2201, 2973, 3761
Bme18I GGWCC 4 cut(s) 51, 311, 788, 1803
BmeT110I CYCGRG 2 cut(s) 321, 1550
BmgT120I GGNCC 4 cut(s) 51, 311, 788, 1803
BmiI GGNNCC 4 cut(s) 153, 205, 366, 1312
BmrFI CCNGG 9 cut(s) 404, 413, 807, 812, 1551, 1552, 2201, 2973, 3761
BmsI GCATC 8 cut(s) 653, 910, 1249, 1441, 2018, 2571, 3435, 3632
BoxI GACNNNNGTC 1 cut(s) 2059
BpiI GAAGAC 1 cut(s) 2945
BpmI CTGGAG 3 cut(s) 49, 127, 2321
Bpu14I TTCGAA 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 2596
BpuMI CCSGG 4 cut(s) 413, 812, 1551, 1552
Bsa29I ATCGAT 2 cut(s) 176, 2544
BsaBI GATNNNNATC 1 cut(s) 182
BsaJI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2780, 3535
BsaWI WCCGGW 1 cut(s) 3498
BsaXI ACNNNNNCTCC 2 cut(s) 1385, 1415
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
Bse118I RCCGGY 1 cut(s) 3795
Bse1I ACTGG 4 cut(s) 841, 1337, 2106, 2304
Bse3DI GCAATG 6 cut(s) 76, 82, 423, 611, 1277, 1333
Bse8I GATNNNNATC 1 cut(s) 182
BseBI CCWGG 5 cut(s) 404, 807, 2201, 2973, 3761
BseCI ATCGAT 2 cut(s) 176, 2544
BseDI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2780, 3535
BseGI GGATG 8 cut(s) 127, 377, 644, 877, 2586, 2744, 2776, 3601
BseJI GATNNNNATC 1 cut(s) 182
BseLI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
BseMI GCAATG 6 cut(s) 76, 82, 423, 611, 1277, 1333
BseMII CTCAG 8 cut(s) 678, 1469, 1925, 2004, 2132, 2232, 2469, 2592
BseNI ACTGG 4 cut(s) 841, 1337, 2106, 2304
BseRI GAGGAG 5 cut(s) 830, 1046, 2258, 3212, 3747
BseSI GKGCMC 2 cut(s) 96, 2089
BseXI GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BseYI CCCAGC 2 cut(s) 187, 938
BsgI GTGCAG 5 cut(s) 1214, 1248, 2082, 2177, 2763
Bsh1236I CGCG 1 cut(s) 1383
BshFI GGCC 5 cut(s) 810, 1044, 2319, 3772, 3795
BshNI GGYRCC 3 cut(s) 151, 203, 1310
BshVI ATCGAT 2 cut(s) 176, 2544
BsiHKAI GWGCWC 2 cut(s) 96, 549
BsiHKCI CYCGRG 2 cut(s) 321, 1550
BsiSI CCGG 5 cut(s) 412, 811, 1551, 3499, 3796
BslFI GGGAC 1 cut(s) 1584
BslI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
BsmAI GTCTC 4 cut(s) 1248, 1632, 2269, 3593
BsmBI CGTCTC 1 cut(s) 1248
BsmFI GGGAC 1 cut(s) 1584
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 5 cut(s) 810, 1044, 2319, 3772, 3795
BsoBI CYCGRG 2 cut(s) 321, 1550
Bsp119I TTCGAA 1 cut(s) 882
Bsp1286I GDGCHC 5 cut(s) 96, 549, 1007, 2089, 2593
Bsp1407I TGTACA 1 cut(s) 1879
Bsp19I CCATGG 1 cut(s) 2780
BspACI CCGC 8 cut(s) 105, 1555, 1786, 1806, 1915, 2282, 3398, 3769
BspANI GGCC 5 cut(s) 810, 1044, 2319, 3772, 3795
BspCNI CTCAG 8 cut(s) 679, 1470, 1924, 2005, 2133, 2233, 2470, 2593
BspDI ATCGAT 2 cut(s) 176, 2544
BspFNI CGCG 1 cut(s) 1383
BspHI TCATGA 1 cut(s) 2353
BspLI GGNNCC 4 cut(s) 153, 205, 366, 1312
BspMAI CTGCAG 1 cut(s) 2998
BspMI ACCTGC 1 cut(s) 1685
BspQI GCTCTTC 2 cut(s) 1758, 2108
BspT104I TTCGAA 1 cut(s) 882
BspT107I GGYRCC 3 cut(s) 151, 203, 1310
BspTI CTTAAG 1 cut(s) 2090
BsrBI CCGCTC 1 cut(s) 3769
BsrDI GCAATG 6 cut(s) 76, 82, 423, 611, 1277, 1333
BsrFI RCCGGY 1 cut(s) 3795
BsrGI TGTACA 1 cut(s) 1879
BsrI ACTGG 4 cut(s) 841, 1337, 2106, 2304
BssAI RCCGGY 1 cut(s) 3795
BssECI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2780, 3535
BssT1I CCWWGG 4 cut(s) 164, 791, 2780, 3535
Bst2UI CCWGG 5 cut(s) 404, 807, 2201, 2973, 3761
Bst4CI ACNGT 8 cut(s) 426, 728, 913, 2130, 2770, 3084, 3175, 3327
Bst6I CTCTTC 3 cut(s) 1246, 1758, 2108
BstAFI CTTAAG 1 cut(s) 2090
BstAPI GCANNNNNTGC 2 cut(s) 197, 713
BstAUI TGTACA 1 cut(s) 1879
BstBI TTCGAA 1 cut(s) 882
BstC8I GCNNGC 6 cut(s) 1130, 2041, 2467, 2835, 3400, 3797
BstDSI CCRYGG 1 cut(s) 2780
BstENI CCTNNNNNAGG 1 cut(s) 2618
BstF5I GGATG 8 cut(s) 127, 377, 644, 877, 2586, 2744, 2776, 3601
BstFNI CGCG 1 cut(s) 1383
BstH2I RGCGCY 1 cut(s) 2647
BstHHI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
BstMAI GTCTC 4 cut(s) 1248, 1632, 2269, 3593
BstMWI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
BstNI CCWGG 5 cut(s) 404, 807, 2201, 2973, 3761
BstNSI RCATGY 2 cut(s) 349, 2837
BstPAI GACNNNNGTC 1 cut(s) 2059
BstSCI CCNGG 9 cut(s) 402, 411, 805, 810, 1549, 1550, 2199, 2971, 3759
BstSFI CTRYAG 2 cut(s) 1173, 2994
BstSLI GKGCMC 2 cut(s) 96, 2089
BstUI CGCG 1 cut(s) 1383
BstV1I GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BstV2I GAAGAC 1 cut(s) 2945
BstX2I RGATCY 6 cut(s) 10, 364, 1069, 1180, 3275, 3358
BstYI RGATCY 6 cut(s) 10, 364, 1069, 1180, 3275, 3358
Bsu15I ATCGAT 2 cut(s) 176, 2544
BsuI GTATCC 2 cut(s) 76, 2888
BsuRI GGCC 5 cut(s) 810, 1044, 2319, 3772, 3795
BsuTUI ATCGAT 2 cut(s) 176, 2544
BtgI CCRYGG 1 cut(s) 2780
BtsCI GGATG 8 cut(s) 127, 377, 644, 877, 2586, 2744, 2776, 3601
BtsI GCAGTG 2 cut(s) 1123, 2160
BtsIMutI CAGTG 7 cut(s) 918, 1123, 1509, 1519, 2099, 2160, 3080
BveI ACCTGC 1 cut(s) 1685
Cac8I GCNNGC 6 cut(s) 1130, 2041, 2467, 2835, 3400, 3797
CaiI CAGNNNCTG 3 cut(s) 917, 2933, 3395
CciI TCATGA 1 cut(s) 2353
CfoI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
Cfr10I RCCGGY 1 cut(s) 3795
Cfr13I GGNCC 4 cut(s) 51, 311, 788, 1803
Cfr9I CCCGGG 1 cut(s) 1550
ClaI ATCGAT 2 cut(s) 176, 2544
CpoI CGGWCCG 1 cut(s) 1803
CseI GACGC 3 cut(s) 178, 3104, 3562
CsiI ACCWGGT 2 cut(s) 402, 2199
Csp6I GTAC 5 cut(s) 1311, 1880, 2238, 2876, 3309
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
CspI CGGWCCG 1 cut(s) 1803
CviQI GTAC 5 cut(s) 1311, 1880, 2238, 2876, 3309
DraI TTTAAA 5 cut(s) 759, 1087, 1141, 1995, 3007
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 2 cut(s) 3770, 3793
Eam1104I CTCTTC 3 cut(s) 1246, 1758, 2108
EarI CTCTTC 3 cut(s) 1246, 1758, 2108
EciI GGCGGA 1 cut(s) 1570
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 4 cut(s) 164, 791, 2780, 3535
Eco24I GRGCYC 3 cut(s) 549, 1007, 2593
Eco32I GATATC 3 cut(s) 184, 2542, 3130
Eco47I GGWCC 4 cut(s) 51, 311, 788, 1803
Eco47III AGCGCT 1 cut(s) 2645
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 1495, 3302
Eco88I CYCGRG 2 cut(s) 321, 1550
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 2618
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 1336, 1498
EcoRII CCWGG 5 cut(s) 402, 805, 2199, 2971, 3759
EcoRV GATATC 3 cut(s) 184, 2542, 3130
EcoT14I CCWWGG 4 cut(s) 164, 791, 2780, 3535
EcoT22I ATGCAT 1 cut(s) 3625
EcoT38I GRGCYC 3 cut(s) 549, 1007, 2593
ErhI CCWWGG 4 cut(s) 164, 791, 2780, 3535
Esp3I CGTCTC 1 cut(s) 1248
FalI AAGNNNNNCTT 6 cut(s) 593, 625, 2009, 2041, 2102, 2134
FaqI GGGAC 1 cut(s) 1584
FauI CCCGC 1 cut(s) 3391
FauNDI CATATG 2 cut(s) 895, 2812
FbaI TGATCA 1 cut(s) 1924
Fnu4HI GCNGC 6 cut(s) 1092, 1263, 1786, 2097, 2487, 3770
FokI GGATG 8 cut(s) 134, 364, 631, 884, 2593, 2751, 2763, 3608
FriOI GRGCYC 3 cut(s) 549, 1007, 2593
Fsp4HI GCNGC 6 cut(s) 1092, 1263, 1786, 2097, 2487, 3770
FspBI CTAG 8 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597, 2985
GlaI GCGC 5 cut(s) 30, 1384, 1784, 2378, 2645
GluI GCNGC 6 cut(s) 1092, 1263, 1786, 2097, 2487, 3770
GsaI CCCAGC 2 cut(s) 191, 942
GsuI CTGGAG 3 cut(s) 49, 127, 2321
HaeII RGCGCY 1 cut(s) 2647
HaeIII GGCC 5 cut(s) 810, 1044, 2319, 3772, 3795
HapII CCGG 5 cut(s) 412, 811, 1551, 3499, 3796
HgaI GACGC 3 cut(s) 178, 3104, 3562
HhaI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
Hin6I GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
HinP1I GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
HincII GTYRAC 2 cut(s) 2457, 3407
HindII GTYRAC 2 cut(s) 2457, 3407
HindIII AAGCTT 5 cut(s) 484, 845, 2231, 2390, 2417
HpaII CCGG 5 cut(s) 412, 811, 1551, 3499, 3796
HphI GGTGA 3 cut(s) 1005, 1745, 3272
Hpy166II GTNNAC 6 cut(s) 94, 333, 2457, 2766, 3407, 3482
Hpy8I GTNNAC 6 cut(s) 94, 333, 2457, 2766, 3407, 3482
Hpy99I CGWCG 2 cut(s) 1825, 3237
HpyAV CCTTC 3 cut(s) 2417, 3043, 3101
HpyCH4III ACNGT 8 cut(s) 426, 728, 913, 2130, 2770, 3084, 3175, 3327
HpyCH4IV ACGT 3 cut(s) 2901, 3210, 3242
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
HpySE526I ACGT 3 cut(s) 2901, 3210, 3242
HspAI GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
KpnI GGTACC 1 cut(s) 1314
KroI GCCGGC 1 cut(s) 3795
KroNI GCCGGC 1 cut(s) 3797
Ksp22I TGATCA 1 cut(s) 1924
LguI GCTCTTC 2 cut(s) 1758, 2108
LmnI GCTCC 5 cut(s) 68, 146, 552, 2302, 3199
Lsp1109I GCAGC 4 cut(s) 1103, 1249, 2083, 2473
LweI GCATC 8 cut(s) 653, 910, 1249, 1441, 2018, 2571, 3435, 3632
MabI ACCWGGT 2 cut(s) 402, 2199
MaeI CTAG 8 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597, 2985
MaeII ACGT 3 cut(s) 2901, 3210, 3242
MaeIII GTNAC 4 cut(s) 398, 997, 1418, 3435
MbiI CCGCTC 1 cut(s) 3769
MfeI CAATTG 3 cut(s) 1211, 1928, 3568
MflI RGATCY 6 cut(s) 10, 364, 1069, 1180, 3275, 3358
MhlI GDGCHC 5 cut(s) 96, 549, 1007, 2089, 2593
MlyI GAGTC 3 cut(s) 452, 747, 1169
MmeI TCCRAC 3 cut(s) 490, 1383, 1863
Mph1103I ATGCAT 1 cut(s) 3625
MroNI GCCGGC 1 cut(s) 3795
MroXI GAANNNNTTC 3 cut(s) 425, 1353, 2631
MslI CAYNNNNRTG 3 cut(s) 1767, 2811, 3254
MspA1I CMGCKG 1 cut(s) 1915
MspCI CTTAAG 1 cut(s) 2090
MspI CCGG 5 cut(s) 412, 811, 1551, 3499, 3796
MspR9I CCNGG 9 cut(s) 404, 413, 807, 812, 1551, 1552, 2201, 2973, 3761
MunI CAATTG 3 cut(s) 1211, 1928, 3568
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 5 cut(s) 404, 807, 2201, 2973, 3761
MvnI CGCG 1 cut(s) 1383
MwoI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
NaeI GCCGGC 1 cut(s) 3797
NciI CCSGG 4 cut(s) 413, 812, 1551, 1552
NcoI CCATGG 1 cut(s) 2780
NdeI CATATG 2 cut(s) 895, 2812
NgoMIV GCCGGC 1 cut(s) 3795
NlaIV GGNNCC 4 cut(s) 153, 205, 366, 1312
NmuCI GTSAC 2 cut(s) 398, 3435
NsiI ATGCAT 1 cut(s) 3625
NspI RCATGY 2 cut(s) 349, 2837
NspV TTCGAA 1 cut(s) 882
PacI TTAATTAA 1 cut(s) 615
PaeI GCATGC 1 cut(s) 2837
PaeR7I CTCGAG 1 cut(s) 321
PagI TCATGA 1 cut(s) 2353
PaqCI CACCTGC 1 cut(s) 1685
PciSI GCTCTTC 2 cut(s) 1758, 2108
PctI GAATGC 1 cut(s) 989
PdiI GCCGGC 1 cut(s) 3797
PdmI GAANNNNTTC 3 cut(s) 425, 1353, 2631
PflFI GACNNNGTC 1 cut(s) 3325
PflMI CCANNNNNTGG 1 cut(s) 2206
PfoI TCCNGGA 1 cut(s) 3759
PkrI GCNGC 6 cut(s) 1093, 1264, 1787, 2098, 2488, 3771
PleI GAGTC 3 cut(s) 452, 746, 1168
PpsI GAGTC 3 cut(s) 452, 746, 1168
PpuMI RGGWCCY 1 cut(s) 51
PshAI GACNNNNGTC 1 cut(s) 2059
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 5 cut(s) 402, 805, 2199, 2971, 3759
PspFI CCCAGC 2 cut(s) 187, 938
PspGI CCWGG 5 cut(s) 402, 805, 2199, 2971, 3759
PspN4I GGNNCC 4 cut(s) 153, 205, 366, 1312
PspPI GGNCC 4 cut(s) 51, 311, 788, 1803
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PstI CTGCAG 1 cut(s) 2998
PstNI CAGNNNCTG 3 cut(s) 917, 2933, 3395
PsuI RGATCY 6 cut(s) 10, 364, 1069, 1180, 3275, 3358
PsyI GACNNNGTC 1 cut(s) 3325
RsaI GTAC 5 cut(s) 1312, 1881, 2239, 2877, 3310
RsaNI GTAC 5 cut(s) 1311, 1880, 2238, 2876, 3309
RseI CAYNNNNRTG 3 cut(s) 1767, 2811, 3254
Rsr2I CGGWCCG 1 cut(s) 1803
RsrII CGGWCCG 1 cut(s) 1803
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 2 cut(s) 1758, 2108
SatI GCNGC 6 cut(s) 1092, 1263, 1786, 2097, 2487, 3770
Sau96I GGNCC 4 cut(s) 51, 311, 788, 1803
SchI GAGTC 3 cut(s) 452, 747, 1169
ScrFI CCNGG 9 cut(s) 404, 413, 807, 812, 1551, 1552, 2201, 2973, 3761
SduI GDGCHC 5 cut(s) 96, 549, 1007, 2089, 2593
SexAI ACCWGGT 2 cut(s) 402, 2199
SfaNI GCATC 8 cut(s) 653, 910, 1249, 1441, 2018, 2571, 3435, 3632
SfcI CTRYAG 2 cut(s) 1173, 2994
Sfr274I CTCGAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 882
SinI GGWCC 4 cut(s) 51, 311, 788, 1803
SlaI CTCGAG 1 cut(s) 321
SmaI CCCGGG 1 cut(s) 1552
SmiMI CAYNNNNRTG 3 cut(s) 1767, 2811, 3254
SmlI CTYRAG 3 cut(s) 321, 2090, 2575
SmoI CTYRAG 3 cut(s) 321, 2090, 2575
SphI GCATGC 1 cut(s) 2837
SrfI GCCCGGGC 1 cut(s) 1552
SsiI CCGC 8 cut(s) 105, 1555, 1786, 1806, 1915, 2282, 3398, 3769
SspI AATATT 2 cut(s) 721, 1287
SspMI CTAG 8 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597, 2985
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 9 cut(s) 402, 411, 805, 810, 1549, 1550, 2199, 2971, 3759
StyI CCWWGG 4 cut(s) 164, 791, 2780, 3535
TaaI ACNGT 8 cut(s) 426, 728, 913, 2130, 2770, 3084, 3175, 3327
TaiI ACGT 3 cut(s) 2904, 3213, 3245
TaqII GACCGA 1 cut(s) 483
TatI WGTACW 1 cut(s) 1879
TauI GCSGC 2 cut(s) 1788, 3772
TscAI CASTG 7 cut(s) 918, 1130, 1509, 1519, 2106, 2160, 3087
TseFI GTSAC 2 cut(s) 398, 3435
TseI GCWGC 4 cut(s) 1091, 1262, 2096, 2486
Tsp45I GTSAC 2 cut(s) 398, 3435
TspGWI ACGGA 1 cut(s) 539
TspMI CCCGGG 1 cut(s) 1550
TspRI CASTG 7 cut(s) 918, 1130, 1509, 1519, 2106, 2160, 3087
Tth111I GACNNNGTC 1 cut(s) 3325
Van91I CCANNNNNTGG 1 cut(s) 2206
Vha464I CTTAAG 1 cut(s) 2090
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 4 cut(s) 51, 311, 788, 1803
XagI CCTNNNNNAGG 1 cut(s) 2618
XapI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
XceI RCATGY 2 cut(s) 349, 2837
XhoI CTCGAG 1 cut(s) 321
XmaI CCCGGG 1 cut(s) 1550
XmnI GAANNNNTTC 3 cut(s) 425, 1353, 2631
XspI CTAG 8 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597, 2985
Zsp2I ATGCAT 1 cut(s) 3625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.