Rh2AG106100
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009582
Physical Location & Seq
Forward (+)
9327412 .. 9333045
5634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG106100.1

Sequence Viewer

Length: 2901 bp
ATGACAATGGATCTACACCCATCAATGGCGCAAAGTGTGAGAACGAGTAGGTCCTCTTTTAGCTCCAGCAATGGCAATGAGGATACTCCTGTGCACAGCTCTACCGCATTTTTGAATGGGGATGATTATGACAGCGATAGCTCCAGCGTGGCACCACCTACACCAAGGACGCTATCGATGGATATCCCAGCAGAACTTGCTGGTGCCATACCCTTGATTGATAGATTTCAGGTAGAGGGATTTTTGAGGTTAATGCAGAAGCAGATTCAGTCTGCTGGAAAACGGGGGTTCTTTTCTAAAAAATCTGTTGGTCCTCAACCTCGAGAAAAGTTCACTTTTGAGGACATGCTGTGCTTCCAAAAGGATCCTATACCTACATCCTTACTTAAAATCAATAGTGACCTGGTTAGCCGGGCAATGAAACTGTTCCAGACCATTCTGAAATACATGGGAATCGACTCATCTGACCGATTTACTCCACCAAGCTTAGATGAAAGGATTGAACTTGTTGGAAAGTTGTATAAGCAAACATTGAAGCGAACAGAGCTCCGTGATGAGCTTTTTGCACAGATTTCCAAACAAACAAGAAATAATCCTGATAAGCAATGCTTAATTAAAGCATGGGAATTGATGTATTTGTGTGCATCCTCCATGCCCCCTAATAAGGACATCGGTGGCTATCTGTCTGAGTATGTCCATAATGTTGCACATGGTGCGAATATTGACTGTGATGTTCGAGTCCTAGCATTAAATACTTTAAATGCTTTGAAGCGTTCTGTCAAGGCTGGACCAAGGCATACAATACCAGGCCGGGAGGAGATAGAAGCACTTTTAACTGGTCGAAAGCTTACAACAATAGTGTTTTTTTTGGATGAAACTTTCGAAGAAATCACATATGACATGGCAACAACAGTGGCTGATGCTGTTGAGGAACTTGCAGGGATAATTAAATTGTCAGCATTTTCTAGCTTTAGTCTGTTTGAATGCCACAAAGTTGTAACGGGCTCGAAATCACCTGACCTTGGAAATGAGGAGTATATTGGCCTAGATGACAACAAATACATAGGGGATCTGTTGGCAGAATTTAAAGCAGCAAAAGATCGAAGTAAAGGAGAAATTTTGCATTGCAAGCTGACATTTAAAAAGAAGCTATTTCGGGAGTCAGATGAAGCTGTAGCAGATCCAATGTTTGTGCAGTTGTCCTATGTTCAATTGCAACATGATTACATCATGGGAAATTATCCTGTGGGAAGAGATGATGCTGCACAGCTTTCTGCATTGCAAATATTGGTTGATATTGGATTTGTTGGTACCCCTGAATCATGCAATGACTGGAATTCGCTTTTGGAACGCTTTCTACCTAGACAAATTGCAATCACACGCGCAAAACGGGAGTGGGAGTTGGATATTCTTTCTCGTTACCATTCAATGCAAAACTTGACAAAAGATGATGCAAGACAACAATTCTTGCGAATACTGAGAACACTTCCTTATGGGAATTCAGTGTTCTTCAGTGTTCGCAAGATAGATGATCCTATTGGACTTCTGCCTGGGCGGATCATTTTGGGAATCAACAAAAGAGGGGTTCATTTTTTTCGTCCTGTTCCAAAGGAATATCTACATTCAGCAGAACTAAGAGACATAATGCAATTTGGTAGTAGCAATACTGCTGTTTTCTTCAAGATGAGAGTTGCAGGTGTTCTTCACATATTCCAATTTGAAACTAAGCAGGGTGAAGAAATTTGCGTTGCTCTTCAAACACATATAAATGATGTCATGTTGCGCCGCTACTCTAAAGCTCGGACCGCTTCTAGTGGTTCAACAAATGGAGATATTTCTAACAATTTGAAGCCCTCTGATGAAGTGCATGAAAAACGTGTACAAGATTTGTCAAAAGCTGTTGAAGAATCTCAGCGGAATGTTGATCAATTGCTGGAAGAATTGCGTGAAAAACAAAAACAAGAAGCGAAACTGCAAGATGATTTGGATAATTTAAAGCAATCATTGGCATCTGAGAAGCAAAACTTGTCAGAAGTTGCAGGCGACCACAATAGACTAAAGTCATTGTGTGATGACAAGGACAAGGCACTTAAGGCTGCACTGGCAGAGAAGAAGAGCTTGGAAGCACAGTTGGCAAAGCTGAGTAATCAAGCAGTGCAGAAAAATGACAAGACAAATCTGGTTGGTGGAGGAAACAACCAGGTTTTGGATAAACTCAAAGATGAGATAAAGCTTCGTACTGAGGAGTTAAAAGAAAAAGAAGAAACTATAAGGAGACTAGCGGACGATAAATTACTACTGGAGCAAACCATATCTGGCCTTGAAAAGAGTAAAGCTGATGAGATTGATTCTCTTGAGAAAACTTTTGAGCAAGAGCGCAAAGCATTAAAGCTTCGAGTTTTTGAACTCGAAAAGAAGCTTGAAGGAGTTAATCAAGAATTAGCTGTTCTAAAGTCAACTCTTGCAAGCAGGAACTCTGAGATTGCTGCATTACAGAATAACCTAAAGGAATTGGATGAATTGAGAGAAATGAAAGAGGATATCGATAGAAAGAATGAGCAAACTGCCTCCCTCTTGAGGATGCAGGGGGCTCAACTAGCTGAGATGGAATCCCTTTACAAGGAGGAACAACTTCTAAGGAAGCGCTATTTCAACACCATAGAAGATATGAAAGGCAAGATCAGAGTTTATTGTAGATTAAGACCTATGAGTGAAAAAGAGATTGCTGAAAAACAAGGATGTGCAGTTTCAAGTATTGATGAGTTTACAGTTGAACATCCATGGAAAGATGAAAAACAGAAACAGCATACATATGATCGTGTATTTGATGAGCATGCCACCCAAGAAGATGTGTTTGAAGATACAAGGGCAAGTTTCTTTCACTCTATCATTTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

966

Amino Acids

109.93

Weight (kDa)

5.96

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 172 - 276 7.7e-26 MyTH4 domain
RA_2 PF21989 282 - 383 1.5e-06 RA like domain
FERM_M PF00373 388 - 504 3.2e-22 FERM central domain
IRS PF02174 514 - 590 5.8e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 874 - 957 6e-18 Microtubule binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1685
Acc36I ACCTGC 1 cut(s) 1685
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 3 cut(s) 151, 203, 1310
AccB7I CCANNNNNTGG 1 cut(s) 2206
AccII CGCG 1 cut(s) 1383
AciI CCGC 6 cut(s) 105, 1555, 1786, 1806, 1915, 2282
AclWI GGATC 7 cut(s) 18, 359, 372, 1077, 1175, 1526, 1565
AcsI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
AcuI CTGAAG 1 cut(s) 1495
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 3 cut(s) 1312, 1881, 2239
AfeI AGCGCT 1 cut(s) 2645
AfiI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 1 cut(s) 1876
AjnI CCWGG 4 cut(s) 402, 805, 1549, 2199
AjuI GAANNNNNNNTTGG 4 cut(s) 1328, 1360, 2102, 2134
Alw21I GWGCWC 2 cut(s) 96, 549
Alw26I GTCTC 2 cut(s) 1632, 2269
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 7 cut(s) 18, 359, 372, 1077, 1175, 1526, 1565
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 321
Aor51HI AGCGCT 1 cut(s) 2645
AoxI GGCC 3 cut(s) 808, 1042, 2317
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 1091, 1262, 2096, 2486
ApoI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
Asp700I GAANNNNTTC 3 cut(s) 425, 1353, 2631
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
AspS9I GGNCC 4 cut(s) 51, 311, 788, 1803
AsuC2I CCSGG 2 cut(s) 413, 812
AsuHPI GGTGA 2 cut(s) 1005, 1745
AsuII TTCGAA 1 cut(s) 882
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 4 cut(s) 51, 311, 788, 1803
BaeGI GKGCMC 1 cut(s) 96
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 3 cut(s) 151, 203, 1310
BanII GRGCYC 3 cut(s) 549, 1007, 2593
Bbv12I GWGCWC 2 cut(s) 96, 549
BbvI GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BccI CCATC 3 cut(s) 28, 172, 2599
BciT130I CCWGG 4 cut(s) 404, 807, 1551, 2201
BciVI GTATCC 1 cut(s) 76
BclI TGATCA 1 cut(s) 1924
BcnI CCSGG 2 cut(s) 413, 812
BcoDI GTCTC 2 cut(s) 1632, 2269
BfaI CTAG 7 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597
BfmI CTRYAG 1 cut(s) 1173
BfoI RGCGCY 1 cut(s) 2647
BfrI CTTAAG 1 cut(s) 2090
BfuAI ACCTGC 1 cut(s) 1685
BfuI GTATCC 1 cut(s) 76
BisI GCNGC 5 cut(s) 1092, 1263, 1786, 2097, 2487
BlsI GCNGC 5 cut(s) 1093, 1264, 1787, 2098, 2488
Bme1390I CCNGG 6 cut(s) 404, 413, 807, 812, 1551, 2201
Bme18I GGWCC 4 cut(s) 51, 311, 788, 1803
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 4 cut(s) 51, 311, 788, 1803
BmiI GGNNCC 4 cut(s) 153, 205, 366, 1312
BmrFI CCNGG 6 cut(s) 404, 413, 807, 812, 1551, 2201
BmsI GCATC 6 cut(s) 653, 910, 1249, 1441, 2018, 2571
BoxI GACNNNNGTC 1 cut(s) 2059
BpmI CTGGAG 3 cut(s) 49, 127, 2321
Bpu14I TTCGAA 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 2375, 2596
BpuMI CCSGG 2 cut(s) 413, 812
Bsa29I ATCGAT 2 cut(s) 176, 2544
BsaBI GATNNNNATC 1 cut(s) 182
BsaJI CCNNGG 5 cut(s) 164, 791, 1021, 1550, 2780
BsaXI ACNNNNNCTCC 2 cut(s) 1385, 1415
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
Bse1I ACTGG 4 cut(s) 841, 1337, 2106, 2304
Bse3DI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
Bse8I GATNNNNATC 1 cut(s) 182
BseBI CCWGG 4 cut(s) 404, 807, 1551, 2201
BseCI ATCGAT 2 cut(s) 176, 2544
BseDI CCNNGG 5 cut(s) 164, 791, 1021, 1550, 2780
BseGI GGATG 8 cut(s) 127, 377, 644, 877, 2521, 2586, 2744, 2776
BseJI GATNNNNATC 1 cut(s) 182
BseLI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
BseMI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
BseMII CTCAG 8 cut(s) 678, 1469, 1925, 2004, 2132, 2232, 2469, 2592
BseNI ACTGG 4 cut(s) 841, 1337, 2106, 2304
BseRI GAGGAG 3 cut(s) 830, 1046, 2258
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 5 cut(s) 1214, 1248, 2082, 2177, 2763
Bsh1236I CGCG 1 cut(s) 1383
BshFI GGCC 3 cut(s) 810, 1044, 2319
BshNI GGYRCC 3 cut(s) 151, 203, 1310
BshVI ATCGAT 2 cut(s) 176, 2544
BsiHKAI GWGCWC 2 cut(s) 96, 549
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 412, 811
BslI CCNNNNNNNGG 6 cut(s) 25, 664, 1022, 2206, 2577, 2620
BsmAI GTCTC 2 cut(s) 1632, 2269
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 3 cut(s) 810, 1044, 2319
BsoBI CYCGRG 1 cut(s) 321
Bsp119I TTCGAA 1 cut(s) 882
Bsp1286I GDGCHC 4 cut(s) 96, 549, 1007, 2593
Bsp1407I TGTACA 1 cut(s) 1879
Bsp19I CCATGG 1 cut(s) 2780
BspACI CCGC 6 cut(s) 105, 1555, 1786, 1806, 1915, 2282
BspANI GGCC 3 cut(s) 810, 1044, 2319
BspCNI CTCAG 8 cut(s) 679, 1470, 1924, 2005, 2133, 2233, 2470, 2593
BspDI ATCGAT 2 cut(s) 176, 2544
BspFNI CGCG 1 cut(s) 1383
BspLI GGNNCC 4 cut(s) 153, 205, 366, 1312
BspMI ACCTGC 1 cut(s) 1685
BspPI GGATC 7 cut(s) 18, 359, 372, 1077, 1175, 1526, 1565
BspQI GCTCTTC 2 cut(s) 1758, 2108
BspT104I TTCGAA 1 cut(s) 882
BspT107I GGYRCC 3 cut(s) 151, 203, 1310
BspTI CTTAAG 1 cut(s) 2090
BsrDI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
BsrGI TGTACA 1 cut(s) 1879
BsrI ACTGG 4 cut(s) 841, 1337, 2106, 2304
BssECI CCNNGG 5 cut(s) 164, 791, 1021, 1550, 2780
BssT1I CCWWGG 4 cut(s) 164, 791, 1021, 2780
Bst2UI CCWGG 4 cut(s) 404, 807, 1551, 2201
Bst4CI ACNGT 5 cut(s) 426, 728, 913, 2130, 2770
Bst6I CTCTTC 3 cut(s) 1246, 1758, 2108
BstAFI CTTAAG 1 cut(s) 2090
BstAPI GCANNNNNTGC 2 cut(s) 197, 713
BstAUI TGTACA 1 cut(s) 1879
BstBI TTCGAA 1 cut(s) 882
BstC8I GCNNGC 4 cut(s) 1130, 2041, 2467, 2835
BstDSI CCRYGG 1 cut(s) 2780
BstENI CCTNNNNNAGG 1 cut(s) 2618
BstF5I GGATG 8 cut(s) 127, 377, 644, 877, 2521, 2586, 2744, 2776
BstFNI CGCG 1 cut(s) 1383
BstH2I RGCGCY 1 cut(s) 2647
BstHHI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
BstMAI GTCTC 2 cut(s) 1632, 2269
BstMWI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
BstNI CCWGG 4 cut(s) 404, 807, 1551, 2201
BstNSI RCATGY 2 cut(s) 349, 2837
BstPAI GACNNNNGTC 1 cut(s) 2059
BstSCI CCNGG 6 cut(s) 402, 411, 805, 810, 1549, 2199
BstSFI CTRYAG 1 cut(s) 1173
BstSLI GKGCMC 1 cut(s) 96
BstUI CGCG 1 cut(s) 1383
BstV1I GCAGC 4 cut(s) 1103, 1249, 2083, 2473
BstX2I RGATCY 4 cut(s) 10, 364, 1069, 1180
BstYI RGATCY 4 cut(s) 10, 364, 1069, 1180
Bsu15I ATCGAT 2 cut(s) 176, 2544
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 3 cut(s) 810, 1044, 2319
BsuTUI ATCGAT 2 cut(s) 176, 2544
BtgI CCRYGG 1 cut(s) 2780
BtsCI GGATG 8 cut(s) 127, 377, 644, 877, 2521, 2586, 2744, 2776
BtsI GCAGTG 1 cut(s) 2160
BtsIMutI CAGTG 5 cut(s) 918, 1509, 1519, 2099, 2160
BveI ACCTGC 1 cut(s) 1685
Cac8I GCNNGC 4 cut(s) 1130, 2041, 2467, 2835
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
Cfr13I GGNCC 4 cut(s) 51, 311, 788, 1803
ClaI ATCGAT 2 cut(s) 176, 2544
CpoI CGGWCCG 1 cut(s) 1803
CseI GACGC 1 cut(s) 178
CsiI ACCWGGT 2 cut(s) 402, 2199
Csp6I GTAC 3 cut(s) 1311, 1880, 2238
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
CspI CGGWCCG 1 cut(s) 1803
CviQI GTAC 3 cut(s) 1311, 1880, 2238
DraI TTTAAA 4 cut(s) 759, 1087, 1141, 1995
DraIII CACNNNGTG 1 cut(s) 713
Eam1104I CTCTTC 3 cut(s) 1246, 1758, 2108
EarI CTCTTC 3 cut(s) 1246, 1758, 2108
EciI GGCGGA 1 cut(s) 1570
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 4 cut(s) 164, 791, 1021, 2780
Eco24I GRGCYC 3 cut(s) 549, 1007, 2593
Eco32I GATATC 2 cut(s) 184, 2542
Eco47I GGWCC 4 cut(s) 51, 311, 788, 1803
Eco47III AGCGCT 1 cut(s) 2645
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 1495
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 2618
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 1336, 1498
EcoRII CCWGG 4 cut(s) 402, 805, 1549, 2199
EcoRV GATATC 2 cut(s) 184, 2542
EcoT14I CCWWGG 4 cut(s) 164, 791, 1021, 2780
EcoT38I GRGCYC 3 cut(s) 549, 1007, 2593
ErhI CCWWGG 4 cut(s) 164, 791, 1021, 2780
FalI AAGNNNNNCTT 6 cut(s) 593, 625, 2009, 2041, 2102, 2134
FauNDI CATATG 2 cut(s) 895, 2812
FbaI TGATCA 1 cut(s) 1924
Fnu4HI GCNGC 5 cut(s) 1092, 1263, 1786, 2097, 2487
FokI GGATG 8 cut(s) 134, 364, 631, 884, 2528, 2593, 2751, 2763
FriOI GRGCYC 3 cut(s) 549, 1007, 2593
Fsp4HI GCNGC 5 cut(s) 1092, 1263, 1786, 2097, 2487
FspBI CTAG 7 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597
GlaI GCGC 5 cut(s) 30, 1384, 1784, 2378, 2645
GluI GCNGC 5 cut(s) 1092, 1263, 1786, 2097, 2487
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 3 cut(s) 49, 127, 2321
HaeII RGCGCY 1 cut(s) 2647
HaeIII GGCC 3 cut(s) 810, 1044, 2319
HapII CCGG 2 cut(s) 412, 811
HgaI GACGC 1 cut(s) 178
HhaI GCGC 5 cut(s) 31, 1385, 1785, 2379, 2646
Hin6I GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
HinP1I GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
HincII GTYRAC 1 cut(s) 2457
HindII GTYRAC 1 cut(s) 2457
HindIII AAGCTT 5 cut(s) 484, 845, 2231, 2390, 2417
HpaII CCGG 2 cut(s) 412, 811
HphI GGTGA 2 cut(s) 1005, 1745
Hpy166II GTNNAC 5 cut(s) 94, 333, 1880, 2457, 2766
Hpy188III TCNNGA 8 cut(s) 323, 430, 596, 1157, 1681, 2354, 2435, 2575
Hpy8I GTNNAC 5 cut(s) 94, 333, 1880, 2457, 2766
HpyAV CCTTC 1 cut(s) 2417
HpyCH4III ACNGT 5 cut(s) 426, 728, 913, 2130, 2770
HpyCH4IV ACGT 1 cut(s) 1876
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
HpySE526I ACGT 1 cut(s) 1876
HspAI GCGC 5 cut(s) 29, 1383, 1783, 2377, 2644
KpnI GGTACC 1 cut(s) 1314
Ksp22I TGATCA 1 cut(s) 1924
LguI GCTCTTC 2 cut(s) 1758, 2108
LmnI GCTCC 4 cut(s) 68, 146, 552, 2302
Lsp1109I GCAGC 4 cut(s) 1103, 1249, 2083, 2473
LweI GCATC 6 cut(s) 653, 910, 1249, 1441, 2018, 2571
MabI ACCWGGT 2 cut(s) 402, 2199
MaeI CTAG 7 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597
MaeII ACGT 1 cut(s) 1876
MaeIII GTNAC 3 cut(s) 398, 997, 1418
MfeI CAATTG 2 cut(s) 1211, 1928
MflI RGATCY 4 cut(s) 10, 364, 1069, 1180
MhlI GDGCHC 4 cut(s) 96, 549, 1007, 2593
MlyI GAGTC 3 cut(s) 452, 747, 1169
MmeI TCCRAC 2 cut(s) 490, 1383
MroXI GAANNNNTTC 3 cut(s) 425, 1353, 2631
MslI CAYNNNNRTG 2 cut(s) 1767, 2811
MspA1I CMGCKG 1 cut(s) 1915
MspCI CTTAAG 1 cut(s) 2090
MspI CCGG 2 cut(s) 412, 811
MspR9I CCNGG 6 cut(s) 404, 413, 807, 812, 1551, 2201
MunI CAATTG 2 cut(s) 1211, 1928
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 4 cut(s) 404, 807, 1551, 2201
MvnI CGCG 1 cut(s) 1383
MwoI GCNNNNNNNGC 9 cut(s) 197, 544, 713, 1129, 1805, 2093, 2102, 2132, 2597
NciI CCSGG 2 cut(s) 413, 812
NcoI CCATGG 1 cut(s) 2780
NdeI CATATG 2 cut(s) 895, 2812
NlaIV GGNNCC 4 cut(s) 153, 205, 366, 1312
NmuCI GTSAC 1 cut(s) 398
NspI RCATGY 2 cut(s) 349, 2837
NspV TTCGAA 1 cut(s) 882
PacI TTAATTAA 1 cut(s) 615
PaeI GCATGC 1 cut(s) 2837
PaeR7I CTCGAG 1 cut(s) 321
PaqCI CACCTGC 1 cut(s) 1685
PciSI GCTCTTC 2 cut(s) 1758, 2108
PctI GAATGC 1 cut(s) 989
PdmI GAANNNNTTC 3 cut(s) 425, 1353, 2631
PfeI GAWTC 7 cut(s) 265, 453, 1319, 1569, 1907, 2348, 2609
PflMI CCANNNNNTGG 1 cut(s) 2206
PkrI GCNGC 5 cut(s) 1093, 1264, 1787, 2098, 2488
PleI GAGTC 3 cut(s) 452, 746, 1168
PpsI GAGTC 3 cut(s) 452, 746, 1168
PpuMI RGGWCCY 1 cut(s) 51
PshAI GACNNNNGTC 1 cut(s) 2059
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 4 cut(s) 402, 805, 1549, 2199
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 4 cut(s) 402, 805, 1549, 2199
PspN4I GGNNCC 4 cut(s) 153, 205, 366, 1312
PspPI GGNCC 4 cut(s) 51, 311, 788, 1803
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 4 cut(s) 10, 364, 1069, 1180
RsaI GTAC 3 cut(s) 1312, 1881, 2239
RsaNI GTAC 3 cut(s) 1311, 1880, 2238
RseI CAYNNNNRTG 2 cut(s) 1767, 2811
Rsr2I CGGWCCG 1 cut(s) 1803
RsrII CGGWCCG 1 cut(s) 1803
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 2 cut(s) 1758, 2108
SatI GCNGC 5 cut(s) 1092, 1263, 1786, 2097, 2487
Sau96I GGNCC 4 cut(s) 51, 311, 788, 1803
SchI GAGTC 3 cut(s) 452, 747, 1169
ScrFI CCNGG 6 cut(s) 404, 413, 807, 812, 1551, 2201
SduI GDGCHC 4 cut(s) 96, 549, 1007, 2593
SexAI ACCWGGT 2 cut(s) 402, 2199
SfaNI GCATC 6 cut(s) 653, 910, 1249, 1441, 2018, 2571
SfcI CTRYAG 1 cut(s) 1173
Sfr274I CTCGAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 882
SinI GGWCC 4 cut(s) 51, 311, 788, 1803
SlaI CTCGAG 1 cut(s) 321
SmiMI CAYNNNNRTG 2 cut(s) 1767, 2811
SmlI CTYRAG 4 cut(s) 321, 2090, 2354, 2575
SmoI CTYRAG 4 cut(s) 321, 2090, 2354, 2575
SphI GCATGC 1 cut(s) 2837
SsiI CCGC 6 cut(s) 105, 1555, 1786, 1806, 1915, 2282
SspI AATATT 2 cut(s) 721, 1287
SspMI CTAG 7 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 6 cut(s) 402, 411, 805, 810, 1549, 2199
StyI CCWWGG 4 cut(s) 164, 791, 1021, 2780
TaaI ACNGT 5 cut(s) 426, 728, 913, 2130, 2770
TaiI ACGT 1 cut(s) 1879
TaqII GACCGA 1 cut(s) 483
TatI WGTACW 1 cut(s) 1879
TauI GCSGC 1 cut(s) 1788
TfiI GAWTC 7 cut(s) 265, 453, 1319, 1569, 1907, 2348, 2609
TscAI CASTG 5 cut(s) 918, 1509, 1519, 2106, 2160
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 4 cut(s) 1091, 1262, 2096, 2486
Tsp45I GTSAC 1 cut(s) 398
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 5 cut(s) 918, 1509, 1519, 2106, 2160
Van91I CCANNNNNTGG 1 cut(s) 2206
Vha464I CTTAAG 1 cut(s) 2090
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 4 cut(s) 51, 311, 788, 1803
XagI CCTNNNNNAGG 1 cut(s) 2618
XapI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
XceI RCATGY 2 cut(s) 349, 2837
XhoI CTCGAG 1 cut(s) 321
XmnI GAANNNNTTC 3 cut(s) 425, 1353, 2631
XspI CTAG 7 cut(s) 743, 966, 1046, 1362, 1812, 2279, 2597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.