Rroxscaffold_2G00145490
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009582
Physical Location & Seq
Reverse (-)
83403870 .. 83412032
8163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145490.1

Sequence Viewer

Length: 3519 bp
ATGACAATGGATCTACACCCATCAACGGCGCAAAGTGTGAGAACCAGTAGGTCCTCTTTTAGCTCCAGCAATGGCAATGAGGATACTCCTGTGCACAGCTCTACGGCCTTTTTGAATGGGGATGATTATGACAGCGATAGCTCCAGCGTGGCACCACCTACTCCAAGGACGCTATCGATGGATATCCCAGCAGAACTAGCTGGTGCCATACCCTTGATTGATAGATTTCAGGTAGAGGGATTTTTGAGGTTAATGCAGAAGCAGATTCAGTCTGCTGGAAAACGGGGGTTCTTTTCTAAAAAATCTGTTGGTCCTCAACCTCGAGAAAAGTTCACTTTTGAGGACATGCTGTGCTTCCAAAAGGATCCTATACCTACATCCTTGCTTAAAATCAATAGTGACCTGGTTAGCCGGGCAATGAAACTGTTCCAGACCATTCTGAAATACATGGGAATCGACTCATCTGACCGATTTACTCCACCAAGCTTAGATGAAAGGATTGAACTTGTTGGAAAGTTGTATAAGCAAACATTGAAGCGAACAGAGCTCCGTGATGAACTTTTTGCACAGATTTCCAAACAAACAAGAAATAATCCTGATAAGCAATGCTTAATTAAAGCATGGGAATTGATGTATTTGTGTGCATCCTCCATGCCCCCTAATAAGGACATCGGTGGCTATCTGTCTGAGTATGTCCATAATGTTGCACATGGTGCGAATATTGACTGTGATGTTCGAGTCCTAGCATTAAATACTTTAAATGCTTTGAAGCGTTCTGTCAAGGCTGGACCAAGGCATACAATACCAGGCCGGGAGGAGATAGAAGCACTTTTAACTGGTCGAAAGCTTACAACAATAGTGTTTTTTTTGGATGAAACTTTCGAAGAAATCACATATGACATGGCAACAACAGTGGCTGATGCTGTTGAGGAACTTGCAGGGATAATTAAATTGTCAGCATTTTCTAGCTTTAGTCTGTTTGAATGCCACAAAGTTGTAACGGGCACGAAATCACCTGACCTCGGAAATGAGGAGTATATTGGCCTAGATGACAACAAATACATAGGGGATCTGTTGGCAGAATTTAAAGCAGCAAAAGATCGAAGTAAAGGAGAAATTTTGCATTGCAAGCTGACATTTAAAAAGAAGCTATTTCGGGAGTCAGATGAAGCTGTAGCAGATCCAATGTTTGTGCAGTTGTCCTATGTTCAATTGCAACATGATTACATCATGGGAAATTATCCTGTGGGAAGAGACGATGCTGCACAGCTTTCTGCATTGCAAATATTGGTTGATATTGGATTCGTTGGTACCCCTGAATCATGCAATGACTGGAATTCGCTTTTGGAACGCTTTCTACCTAGACAAATTGCAATCACACGTGCAAAACGGGAGTGGGAGTTGGATATTCTTTCTCGTTACCATTCAATGCAAAACTTGACAAAAGATGATGCAAGACAACAATTCTTGCGAATACTGAGAACACTTCCTTATGGGAATTCAGTGTTCTTCAGTGTTCGCAAGATAGATGATCCTATTGGACTTCTGCCCGGGCGGATCATTTTGGGAATCAACAAAAGAGGGGTTCATTTTTTTCGTCCTGTTCCAAAGGAATATCTACATTCAGCAGAACTAAGAGACATAATGCAATTTGGGAGTAGCAATACTGCTGTTTTCTTCAAGATGAGAGTTGCAGGTGTTCTTCACATATTCCAATTTGAAACTAAGCAGGGTGAAGAAATTTGTGTTGCTCTTCAAACACATATAAATGATGTCATGTTGCGGCGCTACTCTAAAGCTCGGACCGCTTCTAGTGGTTCGACGAATGGAGATATTTCTAACAATTTGAAGCCCTCTGATGAAGTGCATGAAAAACGTGTACAAGATTTGTCAAAAGCTGTTGAAGAATCTCAGCGGAATGTTGATCAAGTTTTGGATAAACTCAAAGATGAGATAAAGCTTTGTACTGAGGAGTTAAAAGAAAAAGAAGAAACTATAAGGAGACTAGCAGACGATAAATTACTACTGGAGCAAACCATATCTGGCCTTGAAAAGAGCAAAGCTGATGAGATTGATTCTCTTGAGAAAACTTTTGAGCAAGAGCGCAAAGCATTAAAGCTTCGAGTTTTTGAACTTGAAAAGAAGCTTGAAGGAGTTAATCAAGAATTAGCTGTTCTAAAGTCAACTCTTGCAAGCAGGAACTCTGAGATTGCTGCATTACAGAATAACCTAAAGGAATTGGATGAATTGAGAGAAATGAAAGAGGATATCGATAGAAAGAATGAGCAAACTGCCTCCCTCTTGAGGATGCAGGGGGCTCAACTAGCTGAGATGGAATCCCTTTACAAGGAGGAACAACTTCTAAGGAAGCGCTATTTCAACACCATAGAAGATATGAAAGGCAAGATCAGAGTTTATTGTAGATTAAGACCTATGAGTGAAAAAGAGATTGCTGAAAAACAAGGATGTGCAGTTTCAAGTATCGATGAGTTTACAGTTGAACATCCATGGAAAGATGAAAAACAGAAACAGCATACATATGATCGTGTATTTGATGAGCATGCCACCCAAGAAGATTATTTGGTACAATCTGCCGTAGATGGATACAACGTGTGCATATTTGCTTATGGGCAAACAGGTTCTGGGAAGACATATACCATTTATGGTTCAGAAACTAAACCTGGACTTACTCCTAGAGCTACTGCAGAACTTTTTAAAATCTTGAAGCGAGATAGCAACAAGTTCTCATTTTCTTTGAAGGCATATATGGTGGAATTGTATCAAGATACACTGTTAGACCTTCTATTGCCCAAAAATGCAAAGCGTCCAAAATTAGACATCAAAAAGGATTCAAAGGGCATGGTATCAGTTGAAAATATAACGGTTCTATCAATTTCGACACATGAGGAACTGAAAAACGTCATTCAAAGAGGATCTGAACGACGGCATACGTCAGGCACTCAAATGAATCAAGAAAGTTCAAGATCTCACCTCATAGTGTCGGTTATTATTGAAAGTACCAATCTTCAGACGCAGTCTGTTGCAAGAGGAAAGCTAAGTTTTGTGGATCTTGCTGGTTCGGAAAGAATAAAGAAGTCAGGTTCTGCGGGCAGTCAACTCAAAGAAGCTCAAAGCATCAACAAGTCACTTTCAGCACTCGGAGATGTGATTGGTGCTTTATCTTCTGGTGGACAGCACATACCCTACCGGAATCACAAACTAACAATGTTGATGAGTGATTCCCTTGGTGGTAATGCCAAGACGCTTATGTTTGTCAATTGTTCTCCTGCCGAATCAAACATGGATGAGACATATAATTCTCTAATGTATGCATCAAGAGTTCGAGCAATTGTGAATGATCCAAGCAAGAATGTATCTTCAAAAGAGATCATGCGACTGAAAAAGTTGGTTTCTTATTGGAAAGAACAAGCTGGAAAGAGAGGAGAAGATGAGGATTTAGAAGAAATCCAGGACGAGCGGCTACCAAGAGAGAAAGGAGATGGCCGGCACTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

1172

Amino Acids

132.58

Weight (kDa)

6.59

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 172 - 276 1e-25 MyTH4 domain
RA_2 PF21989 282 - 383 1.9e-06 RA like domain
FERM_M PF00373 388 - 504 4.1e-22 FERM central domain
IRS PF02174 514 - 590 7.5e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 783 - 935 2.7e-41 Microtubule binding
Kinesin PF00225 808 - 1117 2.9e-104 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1685
Acc36I ACCTGC 1 cut(s) 1685
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 3 cut(s) 151, 203, 1310
AccBSI CCGCTC 1 cut(s) 3478
AciI CCGC 6 cut(s) 1555, 1783, 1806, 1915, 3107, 3478
AcoI YGGCCR 1 cut(s) 3502
AcsI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
AcuI CTGAAG 2 cut(s) 1495, 3011
AcvI CACGTG 1 cut(s) 1382
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 5 cut(s) 1312, 1881, 1966, 2586, 3019
AfeI AGCGCT 1 cut(s) 2372
AfiI CCNNNNNNNGG 5 cut(s) 25, 664, 1022, 2304, 2347
AflIII ACRYGT 3 cut(s) 1379, 1876, 2610
AjnI CCWGG 4 cut(s) 402, 805, 2680, 3468
AjuI GAANNNNNNNTTGG 2 cut(s) 1328, 1360
Alw21I GWGCWC 2 cut(s) 96, 549
Alw26I GTCTC 4 cut(s) 1248, 1632, 1996, 3302
Alw44I GTGCAC 1 cut(s) 92
AlwNI CAGNNNCTG 3 cut(s) 917, 2642, 3104
Ama87I CYCGRG 2 cut(s) 321, 1550
Aor51HI AGCGCT 1 cut(s) 2372
AoxI GGCC 5 cut(s) 105, 808, 1042, 2044, 3502
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 3 cut(s) 1091, 1262, 2213
ApoI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
Asp700I GAANNNNTTC 3 cut(s) 425, 1353, 2358
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 4 cut(s) 31, 1788, 2106, 2373
AspS9I GGNCC 4 cut(s) 51, 311, 788, 1803
AsuC2I CCSGG 4 cut(s) 413, 812, 1551, 1552
AsuHPI GGTGA 3 cut(s) 1005, 1745, 2981
AsuII TTCGAA 1 cut(s) 882
AvaI CYCGRG 2 cut(s) 321, 1550
AvaII GGWCC 4 cut(s) 51, 311, 788, 1803
BaeGI GKGCMC 2 cut(s) 96, 1007
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 3 cut(s) 151, 203, 1310
BanII GRGCYC 2 cut(s) 549, 2320
BbrPI CACGTG 1 cut(s) 1382
BbsI GAAGAC 1 cut(s) 2654
Bbv12I GWGCWC 2 cut(s) 96, 549
BbvI GCAGC 3 cut(s) 1103, 1249, 2200
BccI CCATC 5 cut(s) 28, 172, 2326, 2594, 3494
BceAI ACGGC 4 cut(s) 42, 120, 2579, 2960
BciT130I CCWGG 4 cut(s) 404, 807, 2682, 3470
BciVI GTATCC 2 cut(s) 76, 2597
BclI TGATCA 1 cut(s) 1924
BcnI CCSGG 4 cut(s) 413, 812, 1551, 1552
BcoDI GTCTC 4 cut(s) 1248, 1632, 1996, 3302
BfaI CTAG 9 cut(s) 197, 743, 966, 1046, 1362, 1812, 2006, 2324, 2694
BfmI CTRYAG 2 cut(s) 1173, 2703
BfoI RGCGCY 2 cut(s) 1789, 2374
BfuAI ACCTGC 1 cut(s) 1685
BfuI GTATCC 2 cut(s) 76, 2597
BglII AGATCT 1 cut(s) 2984
BisI GCNGC 5 cut(s) 1092, 1263, 1784, 2214, 3479
BlsI GCNGC 5 cut(s) 1093, 1264, 1785, 2215, 3480
Bme1390I CCNGG 8 cut(s) 404, 413, 807, 812, 1551, 1552, 2682, 3470
Bme18I GGWCC 4 cut(s) 51, 311, 788, 1803
BmeT110I CYCGRG 2 cut(s) 321, 1550
BmgT120I GGNCC 4 cut(s) 51, 311, 788, 1803
BmiI GGNNCC 4 cut(s) 153, 205, 366, 1312
BmrFI CCNGG 8 cut(s) 404, 413, 807, 812, 1551, 1552, 2682, 3470
BmsI GCATC 7 cut(s) 653, 910, 1249, 1441, 2298, 3144, 3341
BpiI GAAGAC 1 cut(s) 2654
BpmI CTGGAG 3 cut(s) 49, 127, 2048
Bpu14I TTCGAA 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 2102, 2323
BpuMI CCSGG 4 cut(s) 413, 812, 1551, 1552
Bsa29I ATCGAT 3 cut(s) 176, 2271, 2484
BsaAI YACGTR 1 cut(s) 1382
BsaBI GATNNNNATC 1 cut(s) 182
BsaJI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2507, 3244
BsaWI WCCGGW 1 cut(s) 3207
BsaXI ACNNNNNCTCC 2 cut(s) 1385, 1415
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 664, 1022, 2304, 2347
Bse118I RCCGGY 1 cut(s) 3504
Bse1I ACTGG 4 cut(s) 45, 841, 1337, 2031
Bse3DI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
Bse8I GATNNNNATC 1 cut(s) 182
BseBI CCWGG 4 cut(s) 404, 807, 2682, 3470
BseCI ATCGAT 3 cut(s) 176, 2271, 2484
BseDI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2507, 3244
BseGI GGATG 9 cut(s) 127, 377, 644, 877, 2248, 2313, 2471, 2503, 3310
BseJI GATNNNNATC 1 cut(s) 182
BseLI CCNNNNNNNGG 5 cut(s) 25, 664, 1022, 2304, 2347
BseMI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
BseMII CTCAG 6 cut(s) 678, 1469, 1925, 1959, 2196, 2319
BseNI ACTGG 4 cut(s) 45, 841, 1337, 2031
BseRI GAGGAG 4 cut(s) 830, 1046, 1985, 3456
BseSI GKGCMC 2 cut(s) 96, 1007
BseXI GCAGC 3 cut(s) 1103, 1249, 2200
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 3 cut(s) 1214, 1248, 2490
BshFI GGCC 5 cut(s) 107, 810, 1044, 2046, 3504
BshNI GGYRCC 3 cut(s) 151, 203, 1310
BshVI ATCGAT 3 cut(s) 176, 2271, 2484
BsiHKAI GWGCWC 2 cut(s) 96, 549
BsiHKCI CYCGRG 2 cut(s) 321, 1550
BsiSI CCGG 5 cut(s) 412, 811, 1551, 3208, 3505
BslI CCNNNNNNNGG 5 cut(s) 25, 664, 1022, 2304, 2347
BsmAI GTCTC 4 cut(s) 1248, 1632, 1996, 3302
BsmBI CGTCTC 1 cut(s) 1248
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 5 cut(s) 107, 810, 1044, 2046, 3504
BsoBI CYCGRG 2 cut(s) 321, 1550
Bsp119I TTCGAA 1 cut(s) 882
Bsp1286I GDGCHC 4 cut(s) 96, 549, 1007, 2320
Bsp1407I TGTACA 1 cut(s) 1879
Bsp19I CCATGG 1 cut(s) 2507
BspACI CCGC 6 cut(s) 1555, 1783, 1806, 1915, 3107, 3478
BspANI GGCC 5 cut(s) 107, 810, 1044, 2046, 3504
BspCNI CTCAG 6 cut(s) 679, 1470, 1924, 1960, 2197, 2320
BspDI ATCGAT 3 cut(s) 176, 2271, 2484
BspLI GGNNCC 4 cut(s) 153, 205, 366, 1312
BspMAI CTGCAG 1 cut(s) 2707
BspMI ACCTGC 1 cut(s) 1685
BspQI GCTCTTC 1 cut(s) 1758
BspT104I TTCGAA 1 cut(s) 882
BspT107I GGYRCC 3 cut(s) 151, 203, 1310
BsrBI CCGCTC 1 cut(s) 3478
BsrDI GCAATG 7 cut(s) 76, 82, 423, 611, 1123, 1277, 1333
BsrFI RCCGGY 1 cut(s) 3504
BsrGI TGTACA 1 cut(s) 1879
BsrI ACTGG 4 cut(s) 45, 841, 1337, 2031
BssAI RCCGGY 1 cut(s) 3504
BssECI CCNNGG 6 cut(s) 164, 791, 1021, 1550, 2507, 3244
BssT1I CCWWGG 4 cut(s) 164, 791, 2507, 3244
Bst2UI CCWGG 4 cut(s) 404, 807, 2682, 3470
Bst4CI ACNGT 6 cut(s) 426, 728, 913, 2497, 2793, 2884
Bst6I CTCTTC 2 cut(s) 1246, 1758
BstAPI GCANNNNNTGC 1 cut(s) 713
BstAUI TGTACA 1 cut(s) 1879
BstBAI YACGTR 1 cut(s) 1382
BstBI TTCGAA 1 cut(s) 882
BstC8I GCNNGC 5 cut(s) 1130, 2194, 2562, 3109, 3506
BstDSI CCRYGG 1 cut(s) 2507
BstENI CCTNNNNNAGG 1 cut(s) 2345
BstF5I GGATG 9 cut(s) 127, 377, 644, 877, 2248, 2313, 2471, 2503, 3310
BstH2I RGCGCY 2 cut(s) 1789, 2374
BstHHI GCGC 4 cut(s) 31, 1788, 2106, 2373
BstMAI GTCTC 4 cut(s) 1248, 1632, 1996, 3302
BstMWI GCNNNNNNNGC 6 cut(s) 197, 544, 713, 1129, 1805, 2324
BstNI CCWGG 4 cut(s) 404, 807, 2682, 3470
BstNSI RCATGY 2 cut(s) 349, 2564
BstSCI CCNGG 8 cut(s) 402, 411, 805, 810, 1549, 1550, 2680, 3468
BstSFI CTRYAG 2 cut(s) 1173, 2703
BstSLI GKGCMC 2 cut(s) 96, 1007
BstV1I GCAGC 3 cut(s) 1103, 1249, 2200
BstV2I GAAGAC 1 cut(s) 2654
BstX2I RGATCY 7 cut(s) 10, 364, 1069, 1180, 2933, 2984, 3067
BstYI RGATCY 7 cut(s) 10, 364, 1069, 1180, 2933, 2984, 3067
Bsu15I ATCGAT 3 cut(s) 176, 2271, 2484
BsuI GTATCC 2 cut(s) 76, 2597
BsuRI GGCC 5 cut(s) 107, 810, 1044, 2046, 3504
BsuTUI ATCGAT 3 cut(s) 176, 2271, 2484
BtgI CCRYGG 1 cut(s) 2507
BtsCI GGATG 9 cut(s) 127, 377, 644, 877, 2248, 2313, 2471, 2503, 3310
BtsIMutI CAGTG 4 cut(s) 918, 1509, 1519, 2789
BveI ACCTGC 1 cut(s) 1685
Cac8I GCNNGC 5 cut(s) 1130, 2194, 2562, 3109, 3506
CaiI CAGNNNCTG 3 cut(s) 917, 2642, 3104
CfoI GCGC 4 cut(s) 31, 1788, 2106, 2373
Cfr10I RCCGGY 1 cut(s) 3504
Cfr13I GGNCC 4 cut(s) 51, 311, 788, 1803
Cfr9I CCCGGG 1 cut(s) 1550
ClaI ATCGAT 3 cut(s) 176, 2271, 2484
CpoI CGGWCCG 1 cut(s) 1803
CseI GACGC 4 cut(s) 178, 2813, 3040, 3271
CsiI ACCWGGT 1 cut(s) 402
Csp6I GTAC 5 cut(s) 1311, 1880, 1965, 2585, 3018
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
CspI CGGWCCG 1 cut(s) 1803
CviQI GTAC 5 cut(s) 1311, 1880, 1965, 2585, 3018
DraI TTTAAA 4 cut(s) 759, 1087, 1141, 2716
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 3502
Eam1104I CTCTTC 2 cut(s) 1246, 1758
EarI CTCTTC 2 cut(s) 1246, 1758
EciI GGCGGA 1 cut(s) 1570
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 4 cut(s) 164, 791, 2507, 3244
Eco24I GRGCYC 2 cut(s) 549, 2320
Eco32I GATATC 2 cut(s) 184, 2269
Eco47I GGWCC 4 cut(s) 51, 311, 788, 1803
Eco47III AGCGCT 1 cut(s) 2372
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 1495, 3011
Eco72I CACGTG 1 cut(s) 1382
Eco88I CYCGRG 2 cut(s) 321, 1550
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 2345
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 1336, 1498
EcoRII CCWGG 4 cut(s) 402, 805, 2680, 3468
EcoRV GATATC 2 cut(s) 184, 2269
EcoT14I CCWWGG 4 cut(s) 164, 791, 2507, 3244
EcoT22I ATGCAT 1 cut(s) 3334
EcoT38I GRGCYC 2 cut(s) 549, 2320
ErhI CCWWGG 4 cut(s) 164, 791, 2507, 3244
Esp3I CGTCTC 1 cut(s) 1248
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FauI CCCGC 1 cut(s) 3100
FauNDI CATATG 2 cut(s) 895, 2539
FbaI TGATCA 1 cut(s) 1924
Fnu4HI GCNGC 5 cut(s) 1092, 1263, 1784, 2214, 3479
FokI GGATG 9 cut(s) 134, 364, 631, 884, 2255, 2320, 2478, 2490, 3317
FriOI GRGCYC 2 cut(s) 549, 2320
Fsp4HI GCNGC 5 cut(s) 1092, 1263, 1784, 2214, 3479
FspBI CTAG 9 cut(s) 197, 743, 966, 1046, 1362, 1812, 2006, 2324, 2694
GlaI GCGC 4 cut(s) 30, 1787, 2105, 2372
GluI GCNGC 5 cut(s) 1092, 1263, 1784, 2214, 3479
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 3 cut(s) 49, 127, 2048
HaeII RGCGCY 2 cut(s) 1789, 2374
HaeIII GGCC 5 cut(s) 107, 810, 1044, 2046, 3504
HapII CCGG 5 cut(s) 412, 811, 1551, 3208, 3505
HgaI GACGC 4 cut(s) 178, 2813, 3040, 3271
HhaI GCGC 4 cut(s) 31, 1788, 2106, 2373
Hin6I GCGC 4 cut(s) 29, 1786, 2104, 2371
HinP1I GCGC 4 cut(s) 29, 1786, 2104, 2371
HincII GTYRAC 2 cut(s) 2184, 3116
HindII GTYRAC 2 cut(s) 2184, 3116
HindIII AAGCTT 5 cut(s) 484, 845, 1958, 2117, 2144
HpaII CCGG 5 cut(s) 412, 811, 1551, 3208, 3505
HphI GGTGA 3 cut(s) 1005, 1745, 2981
Hpy166II GTNNAC 7 cut(s) 94, 333, 1880, 2184, 2493, 3116, 3191
Hpy8I GTNNAC 7 cut(s) 94, 333, 1880, 2184, 2493, 3116, 3191
Hpy99I CGWCG 2 cut(s) 1825, 2946
HpyAV CCTTC 3 cut(s) 2144, 2752, 2810
HpyCH4III ACNGT 6 cut(s) 426, 728, 913, 2497, 2793, 2884
HpyCH4IV ACGT 5 cut(s) 1381, 1876, 2610, 2919, 2951
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 544, 713, 1129, 1805, 2324
HpySE526I ACGT 5 cut(s) 1381, 1876, 2610, 2919, 2951
HspAI GCGC 4 cut(s) 29, 1786, 2104, 2371
KpnI GGTACC 1 cut(s) 1314
KroI GCCGGC 1 cut(s) 3504
KroNI GCCGGC 1 cut(s) 3506
Ksp22I TGATCA 1 cut(s) 1924
LguI GCTCTTC 1 cut(s) 1758
LmnI GCTCC 4 cut(s) 68, 146, 552, 2029
Lsp1109I GCAGC 3 cut(s) 1103, 1249, 2200
LweI GCATC 7 cut(s) 653, 910, 1249, 1441, 2298, 3144, 3341
MabI ACCWGGT 1 cut(s) 402
MaeI CTAG 9 cut(s) 197, 743, 966, 1046, 1362, 1812, 2006, 2324, 2694
MaeII ACGT 5 cut(s) 1381, 1876, 2610, 2919, 2951
MaeIII GTNAC 4 cut(s) 398, 997, 1418, 3144
MbiI CCGCTC 1 cut(s) 3478
MfeI CAATTG 3 cut(s) 1211, 3277, 3348
MflI RGATCY 7 cut(s) 10, 364, 1069, 1180, 2933, 2984, 3067
MhlI GDGCHC 4 cut(s) 96, 549, 1007, 2320
MlyI GAGTC 3 cut(s) 452, 747, 1169
MmeI TCCRAC 2 cut(s) 490, 1383
Mph1103I ATGCAT 1 cut(s) 3334
MroNI GCCGGC 1 cut(s) 3504
MroXI GAANNNNTTC 3 cut(s) 425, 1353, 2358
MslI CAYNNNNRTG 3 cut(s) 1767, 2538, 2963
MspA1I CMGCKG 1 cut(s) 1915
MspI CCGG 5 cut(s) 412, 811, 1551, 3208, 3505
MspR9I CCNGG 8 cut(s) 404, 413, 807, 812, 1551, 1552, 2682, 3470
MunI CAATTG 3 cut(s) 1211, 3277, 3348
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 4 cut(s) 404, 807, 2682, 3470
MwoI GCNNNNNNNGC 6 cut(s) 197, 544, 713, 1129, 1805, 2324
NaeI GCCGGC 1 cut(s) 3506
NciI CCSGG 4 cut(s) 413, 812, 1551, 1552
NcoI CCATGG 1 cut(s) 2507
NdeI CATATG 2 cut(s) 895, 2539
NgoMIV GCCGGC 1 cut(s) 3504
NlaIV GGNNCC 4 cut(s) 153, 205, 366, 1312
NmuCI GTSAC 2 cut(s) 398, 3144
NsiI ATGCAT 1 cut(s) 3334
NspI RCATGY 2 cut(s) 349, 2564
NspV TTCGAA 1 cut(s) 882
PacI TTAATTAA 1 cut(s) 615
PaeI GCATGC 1 cut(s) 2564
PaeR7I CTCGAG 1 cut(s) 321
PaqCI CACCTGC 1 cut(s) 1685
PciSI GCTCTTC 1 cut(s) 1758
PctI GAATGC 1 cut(s) 989
PdiI GCCGGC 1 cut(s) 3506
PdmI GAANNNNTTC 3 cut(s) 425, 1353, 2358
PflFI GACNNNGTC 1 cut(s) 3034
PfoI TCCNGGA 1 cut(s) 3468
PkrI GCNGC 5 cut(s) 1093, 1264, 1785, 2215, 3480
PleI GAGTC 3 cut(s) 452, 746, 1168
PmaCI CACGTG 1 cut(s) 1382
PmlI CACGTG 1 cut(s) 1382
PpsI GAGTC 3 cut(s) 452, 746, 1168
Ppu21I YACGTR 1 cut(s) 1382
PpuMI RGGWCCY 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 4 cut(s) 402, 805, 2680, 3468
PspCI CACGTG 1 cut(s) 1382
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 4 cut(s) 402, 805, 2680, 3468
PspN4I GGNNCC 4 cut(s) 153, 205, 366, 1312
PspPI GGNCC 4 cut(s) 51, 311, 788, 1803
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PstI CTGCAG 1 cut(s) 2707
PstNI CAGNNNCTG 3 cut(s) 917, 2642, 3104
PsuI RGATCY 7 cut(s) 10, 364, 1069, 1180, 2933, 2984, 3067
PsyI GACNNNGTC 1 cut(s) 3034
RsaI GTAC 5 cut(s) 1312, 1881, 1966, 2586, 3019
RsaNI GTAC 5 cut(s) 1311, 1880, 1965, 2585, 3018
RseI CAYNNNNRTG 3 cut(s) 1767, 2538, 2963
Rsr2I CGGWCCG 1 cut(s) 1803
RsrII CGGWCCG 1 cut(s) 1803
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 1758
SatI GCNGC 5 cut(s) 1092, 1263, 1784, 2214, 3479
Sau96I GGNCC 4 cut(s) 51, 311, 788, 1803
SchI GAGTC 3 cut(s) 452, 747, 1169
ScrFI CCNGG 8 cut(s) 404, 413, 807, 812, 1551, 1552, 2682, 3470
SduI GDGCHC 4 cut(s) 96, 549, 1007, 2320
SexAI ACCWGGT 1 cut(s) 402
SfaNI GCATC 7 cut(s) 653, 910, 1249, 1441, 2298, 3144, 3341
SfcI CTRYAG 2 cut(s) 1173, 2703
Sfr274I CTCGAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 882
SinI GGWCC 4 cut(s) 51, 311, 788, 1803
SlaI CTCGAG 1 cut(s) 321
SmaI CCCGGG 1 cut(s) 1552
SmiMI CAYNNNNRTG 3 cut(s) 1767, 2538, 2963
SmlI CTYRAG 3 cut(s) 321, 2081, 2302
SmoI CTYRAG 3 cut(s) 321, 2081, 2302
SphI GCATGC 1 cut(s) 2564
SrfI GCCCGGGC 1 cut(s) 1552
SsiI CCGC 6 cut(s) 1555, 1783, 1806, 1915, 3107, 3478
SspI AATATT 2 cut(s) 721, 1287
SspMI CTAG 9 cut(s) 197, 743, 966, 1046, 1362, 1812, 2006, 2324, 2694
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 8 cut(s) 402, 411, 805, 810, 1549, 1550, 2680, 3468
StyI CCWWGG 4 cut(s) 164, 791, 2507, 3244
TaaI ACNGT 6 cut(s) 426, 728, 913, 2497, 2793, 2884
TaiI ACGT 5 cut(s) 1384, 1879, 2613, 2922, 2954
TaqII GACCGA 1 cut(s) 483
TatI WGTACW 2 cut(s) 1879, 1964
TauI GCSGC 2 cut(s) 1786, 3481
TscAI CASTG 4 cut(s) 918, 1509, 1519, 2796
TseFI GTSAC 2 cut(s) 398, 3144
TseI GCWGC 3 cut(s) 1091, 1262, 2213
Tsp45I GTSAC 2 cut(s) 398, 3144
TspGWI ACGGA 1 cut(s) 539
TspMI CCCGGG 1 cut(s) 1550
TspRI CASTG 4 cut(s) 918, 1509, 1519, 2796
Tth111I GACNNNGTC 1 cut(s) 3034
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 4 cut(s) 51, 311, 788, 1803
XagI CCTNNNNNAGG 1 cut(s) 2345
XapI RAATTY 5 cut(s) 1082, 1116, 1336, 1498, 1740
XceI RCATGY 2 cut(s) 349, 2564
XhoI CTCGAG 1 cut(s) 321
XmaI CCCGGG 1 cut(s) 1550
XmnI GAANNNNTTC 3 cut(s) 425, 1353, 2358
XspI CTAG 9 cut(s) 197, 743, 966, 1046, 1362, 1812, 2006, 2324, 2694
Zsp2I ATGCAT 1 cut(s) 3334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.