MD15G1226100.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009582
Physical Location & Seq
Forward (+)
18356296 .. 18364714
8419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1226100.v1.1.491

Sequence Viewer

Length: 3798 bp
ATGACAATTGACCTGAACCCGTCAATGTCGCAAAGTGTGAGAACTTTTAGCTCTAGCAATGGAAATGAGGATTCAACTCCTTTACATGGCTCTGCTGCTGCAGCATTTTCGAATGGGGATGACTATGACAGTGATAGCTCCAGCTTGGCACCGCAAACACCAAGGACCTTATCCATGGACATCCCGGCAGAACTTGCTGGTGCCATACCCTTGATTGATAGATTTCAGGTAGAAGGATTTTTAAGGTTGATGCAGAAACAGATTCAGTCTGCTGGAAAACGCGGATTCTTTACGAAAAAATCCGTTGGTCCTCAATCCCGAGAGAAGTTCACTTTTGAGGATATGTTGTGCTACCAAAAGGATCCAATACCTACATCATTACTGAAAATAAATAATGACGTGGTAAGCCGGGCAATGAAACTGTTCCAAATAATTTTGAAGTACATGGGAGTTGATTCATCTGATCGAGTTACTCCAGCAAGCTTAGATGAACGTATAGAGCTTGTTGGAAAGATGTTTAAGCAAACATTGAAGCGAACAGAGCTTCGGGATGAACTTTTTGCTCAAATTTCAAAACAAACAAGAAATAATCCCGATAAGCAATATTTGATCAAAGCATGGGAATTAATGTATCTGTGTTCGTCCTCTATGCCTCCGAGCAAGGACATTGGGGGGTATCTGTCCGAGTATGTCCACAATGTCGCACATGGTGTGGATATTGACTCTGAGGTTCGAGTCCTAGCAGTAAATACATTAAATGCTTTGAAGCGTTCTGTGAAGGCTGGTCCGAGGCATACAATACCAGGCCGCGAGGAGATTGAAGCACTTTTAACCGGTCGAAAGCTAACAACAATTGTGTTTTTCTTGGATGAAACCTTTGAAGAAATCACATATGATATGGCAACAACAGTGGCTGATGCTGTTGAGGAACTTGCAGGGGTAATTAAACTGTCAGCCTTTTCTAGCTTTAGTCTGTTTGAATGCCGTAAAGTTGTAACTGGTTCAAAATCACCTGATCCTGGAAATGAGGAGTATATTGGGTTAGATGACAACAAGTACATAGGGGATCTATTAGCAGAATTCAAGGCAGCAAAGGATCGAAGTAAAGGAGAGATATTGCATTGCAAGCTGATATTTAAGAAAAAGCTGTTTCGGGAGTTGGATGAAGCTGTCACAGATCCAATGTTTGTGCAGTTGTCCTACGTTCAGTTGCAACATGATTATATACTGGGAAATTATCCTGTTGGAAGAGATGATGCTGCGCAGCTTTCTGCATTGCAAATCTTGGTTGACATCGGATTTCAGCGCAATCCTGAATCAAGCACTGACTGGAATTCGCTTTTGGAACGCTTCCTACCCAGACAAATCGCAATCACACGAGCAAAACGAGAGTGGGAGTTTGATATTCTTTCACGTTACCATTTAATGGAAAGTTTGACAAAAGATGATGCAAGACAACAATTTTTGAGAGTACTGAGACAACTTCCTTACGGGAATTCAGTCTTCTTCAGTGTTCGCAAGATTGATGATCCCATTGGACTTCTTCCAGGGAGAATTATTTTGGGCATCAACAAAAGAGGGGTTCATTTTTTCCGTCCAGTTCCAAAGGAATATCTACATTCAGCTGAGCTAAGAGACATAATGCAATTTGGTAGTAGCAATACTGCTGTTTTCTTTAAGATGAGAGTTGCAGGTGTTCTTCACATATTCCAATTTGAAACTAAGCAGGGTGAAGAAATTTGTGTTGCTCTTCAAACACACATAAATGACGTCATGTTGCGGCGGTACACTAAAGCTCGGACTGCTGCTAGTGGTTCAACAATTGGAGATCTTTCTAACAATGTTAAGCCCTCTGATGGTGAAGTGTATGGAAAACGTGTACAGGATTTGTCAAAAGCTGTTGAAGAGTCTCAGAGGAATGCTGATCAATTGCTGGAAGAATTGCGTGAAAAGCAAAAACAAGAAGCAAAAATGCAAGAAGAATTAGAAAGTTTGAAACAATCCTTGGCATCTGAGAAGCAAAACCTGACAGAAGTTAGATGTGATCATGATAGGCTTAGGTCATCGTGTGACGAAAAGGATAAGGCGCTTCAGGCTGCACTGCTAGAGAAAAAAAGCTTGGAAGCAAGGCTGCAGAACTTGGGTAATCAAGTGGCAGAGAAAAGTAACAAAACACAGCAAGTTGGTGGAAATAACCACAAGCTCGAAGACGAGATAAATCTTCGTGGTGAGGAGTTGTTAGCAAAAGAAAAAACTATAAGGAGACTATCTGATGAAAAAATATCATTGGAGCAAAGATTATCTGGGCTCGAAAAGACTAAATCTGTTGAGATTGATTCTATTGAGAAAAAATTTGAGCAAGAGCGCAAAGTGTTAAAGCTTCAAGTGTTCGAACTTGAAAAGAAGCTTGAAGGAGTCAACCAAGAATTAGCAGGTCTTAAGTCAATTCTTGCCACCAAAAACTCTGAGGTTGCTGAATTACAAAGTAGCTTAAAGGAATTGGAGGAACTGAGAGAAATGAAAGAGGACATTGATAGAAAGAACGAGCAAACTGCTGCCATCTTGAGGATGCAAGGAGCTCAACTAGCTGAGATAGAAGCGCTTTACAAAGAAGAACAACTTTTGAGGAAGCGATATTTTAATACTATAGAAGATATGAAAGGCAAGATTAGAGTCTTCTGTCGGTTAAGACCTTTGAGTGAAAAAGAAGTTACAGAAAAGGAAAGAGATGCAGTTAAAAGTGTAGATGAGTTCACAATTGAACATCCATGGAAGGATGACAAATTGAAACAACATATGTATGATCGTGTATTTGATGGTAATGCCACCCAAGAGGATGTATTTGAGGATACAAGGTATTTGGTACAATCTGCAATTGATGGGTATAATGTGTGCATATTTGCGTATGGGCAAACCGGTTCTGGAAAGACATATACCATATACGGTTCAGAAACTAATCCTGGACTTACTCCACGAGCTACTGCAGAGCTGTTTAAAATATTGAGGCGAGAGAACAACAAATTCTCATTTTCGCTAAAGGCATACATGTTGGAGTTGTATCAAGATACACTTGTAGACCTTCTCTTACCGAAAAATGCCAAGCGTTTGAAATTGGAGATCAAGAAAGATTCAAAGGGTATGGTCACGGTTGAGAATGTAACAGTTCTCTCAATTTCATCACACGAGGAACTGAAAAGTGTCATTCAAAGAGGTTCTGAACGAAGGCATGTTTCTGGAACTCAGATGAACGAAGAAAGTTCAAGATCACACCTCATAGTTTCTGTTGTCATTGAAAGTACCAACCTTCAAACTCAATCTGTTGGAAGAGGAAAGCTAAGTTTTGTGGATCTTGCTGGTTCGGAAAGAATAAAGAAGTCAGGCTCTTCAGGCAGTCAACTTAAAGAAGCTCAAAGCATCAACAAGTCACTTTCAGCACTTGGGGATGTTATTAGTTCTTTATCTTCTGGTGGCCAACACATACCGTACAGGAACCATAAGTTAACGATGCTAATGAGTGACTCACTTGGTGGTAACGCCAAGACGCTTATGTTCGTAAATGTTTCTCCTGCTGAATCAAATGTGGAGGAGTCGTACAATTCTCTAATGTATGCATCAAGAGTACGATCAATCGTGAATGATCCAAGCAAAAACGTTTCTTCAAAAGAGATCATGCGGCTGAAGAAGTTGGTTGCATATTGGAAAGAACAAGCAGGCAAGAGAGGAGACGACGAAGATTTGGAAGAAATTCAGGACGAGCGACCGGTGAAAGATAGAGGTGATGGCCGCCATTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000226 GO:0000902 GO:0000904 GO:0001578 GO:0003002 GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005881 GO:0005938 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007267 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030154 GO:0030554 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0036094 GO:0042623 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043531 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0045165 GO:0045168 GO:0046872 GO:0046983 GO:0048468 GO:0048629 GO:0048856 GO:0048869 GO:0051015 GO:0055028 GO:0071840 GO:0071944 GO:0072686 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901265 GO:1901363 GO:1990939
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FERM_M FERM_N Kinesin MyTH4

Protein Analysis

1266

Amino Acids

143.22

Weight (kDa)

6.49

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MyTH4 PF00784 171 - 275 3.1e-27 MyTH4 domain
RA_2 PF21989 281 - 381 3.1e-06 RA like domain
FERM_M PF00373 387 - 503 7.3e-21 FERM central domain
IRS PF02174 513 - 589 8.2e-07 PTB domain (IRS-1 type)
Microtub_bd PF16796 870 - 1028 1.1e-46 Microtubule binding
Kinesin PF00225 895 - 1210 5.2e-109 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1682
AatII GACGTC 1 cut(s) 1773
Acc16I TGCGCA 1 cut(s) 1263
Acc36I ACCTGC 2 cut(s) 1682, 2423
AccB1I GGYRCC 2 cut(s) 148, 200
AccB7I CCANNNNNTGG 1 cut(s) 1426
AccI GTMKAC 1 cut(s) 3075
AccII CGCG 2 cut(s) 282, 810
AciI CCGC 7 cut(s) 152, 282, 808, 1780, 1783, 3673, 3784
AclI AACGTT 1 cut(s) 3651
AclWI GGATC 9 cut(s) 356, 369, 1010, 1074, 1104, 1172, 1523, 3354, 3632
AcoI YGGCCR 2 cut(s) 3469, 3781
AcsI RAATTY 8 cut(s) 567, 1079, 1333, 1495, 1737, 2351, 3020, 3744
AcuI CTGAAG 4 cut(s) 1492, 2075, 3369, 3698
AcyI GRCGYC 1 cut(s) 1770
AdeI CACNNNGTG 2 cut(s) 710, 3527
AfeI AGCGCT 1 cut(s) 2601
AfiI CCNNNNNNNGG 5 cut(s) 86, 1019, 1426, 1856, 2565
AflII CTTAAG 1 cut(s) 2438
AflIII ACRYGT 2 cut(s) 1876, 3045
AgeI ACCGGT 3 cut(s) 833, 2915, 3760
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 4 cut(s) 802, 1018, 1546, 2959
AjuI GAANNNNNNNTTGG 6 cut(s) 1325, 1357, 1988, 2020, 2102, 2134
Alw21I GWGCWC 1 cut(s) 2581
Alw26I GTCTC 5 cut(s) 1471, 1629, 1914, 2257, 3717
AlwI GGATC 9 cut(s) 356, 369, 1010, 1074, 1104, 1172, 1523, 3354, 3632
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 318
Aor51HI AGCGCT 1 cut(s) 2601
AoxI GGCC 3 cut(s) 805, 3469, 3781
ApoI RAATTY 8 cut(s) 567, 1079, 1333, 1495, 1737, 2351, 3020, 3744
AseI ATTAAT 1 cut(s) 626
AsiGI ACCGGT 3 cut(s) 833, 2915, 3760
Asp700I GAANNNNTTC 2 cut(s) 422, 3744
AspLEI GCGC 5 cut(s) 1264, 1308, 2089, 2367, 2602
AspS9I GGNCC 3 cut(s) 165, 308, 785
AsuC2I CCSGG 2 cut(s) 185, 410
AsuHPI GGTGA 6 cut(s) 1002, 1742, 1871, 2240, 3775, 3788
AsuII TTCGAA 2 cut(s) 110, 2391
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 3 cut(s) 165, 308, 785
BaeI ACNNNNGTAYC 2 cut(s) 3057, 3090
BalI TGGCCA 1 cut(s) 3471
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 2 cut(s) 148, 200
BanII GRGCYC 2 cut(s) 2310, 2581
BauI CACGAG 3 cut(s) 1377, 2973, 3182
BbsI GAAGAC 3 cut(s) 1495, 2214, 2668
Bbv12I GWGCWC 1 cut(s) 2581
BccI CCATC 5 cut(s) 1850, 2567, 2810, 2873, 3773
BceAI ACGGC 1 cut(s) 969
BcgI CGANNNNNNTGC 4 cut(s) 90, 124, 2534, 2568
BciT130I CCWGG 4 cut(s) 804, 1020, 1548, 2961
BciVI GTATCC 1 cut(s) 2842
BclI TGATCA 3 cut(s) 609, 1924, 2044
BcnI CCSGG 2 cut(s) 185, 410
BcoDI GTCTC 5 cut(s) 1471, 1629, 1914, 2257, 3717
BfaI CTAG 6 cut(s) 54, 740, 963, 1809, 2105, 2585
BfmI CTRYAG 4 cut(s) 99, 2132, 2646, 2982
BfoI RGCGCY 2 cut(s) 2090, 2603
BfrI CTTAAG 1 cut(s) 2438
BfuAI ACCTGC 2 cut(s) 1682, 2423
BfuI GTATCC 1 cut(s) 2842
BglII AGATCT 1 cut(s) 1828
BlpI GCTNAGC 1 cut(s) 1626
BmcAI AGTACT 1 cut(s) 1473
Bme1390I CCNGG 6 cut(s) 185, 410, 804, 1020, 1548, 2961
Bme18I GGWCC 3 cut(s) 165, 308, 785
BmeT110I CYCGRG 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 165, 308, 785
BmiI GGNNCC 4 cut(s) 150, 202, 363, 3491
BmrFI CCNGG 6 cut(s) 185, 410, 804, 1020, 1548, 2961
BmrI ACTGGG 1 cut(s) 1238
BmuI ACTGGG 1 cut(s) 1238
BpiI GAAGAC 3 cut(s) 1495, 2214, 2668
BpmI CTGGAG 2 cut(s) 124, 459
Bpu10I CCTNAGC 1 cut(s) 2057
Bpu1102I GCTNAGC 1 cut(s) 1626
Bpu14I TTCGAA 2 cut(s) 110, 2391
BpuEI CTTGAG 1 cut(s) 2584
BpuMI CCSGG 2 cut(s) 185, 410
BsaHI GRCGYC 1 cut(s) 1770
BsaJI CCNNGG 6 cut(s) 161, 174, 788, 1547, 2004, 2768
BsaWI WCCGGW 3 cut(s) 833, 2915, 3760
BsaXI ACNNNNNCTCC 2 cut(s) 3575, 3605
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 1019, 1426, 1856, 2565
Bse118I RCCGGY 3 cut(s) 833, 2915, 3760
Bse1I ACTGG 4 cut(s) 1003, 1233, 1334, 1598
Bse3DI GCAATG 4 cut(s) 64, 420, 1120, 1274
BseBI CCWGG 4 cut(s) 804, 1020, 1548, 2961
BseDI CCNNGG 6 cut(s) 161, 174, 788, 1547, 2004, 2768
BseLI CCNNNNNNNGG 5 cut(s) 86, 1019, 1426, 1856, 2565
BseMI GCAATG 4 cut(s) 64, 420, 1120, 1274
BseMII CTCAG 9 cut(s) 717, 1466, 1617, 1925, 2004, 2457, 2501, 2580, 3254
BseNI ACTGG 4 cut(s) 1003, 1233, 1334, 1598
BseRI GAGGAG 5 cut(s) 827, 1043, 2246, 3599, 3735
BsgI GTGCAG 2 cut(s) 1211, 2082
Bsh1236I CGCG 2 cut(s) 282, 810
Bsh1285I CGRYCG 2 cut(s) 838, 3761
BshFI GGCC 3 cut(s) 807, 3471, 3783
BshNI GGYRCC 2 cut(s) 148, 200
BshTI ACCGGT 3 cut(s) 833, 2915, 3760
BsiEI CGRYCG 2 cut(s) 838, 3761
BsiHKAI GWGCWC 1 cut(s) 2581
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 5 cut(s) 185, 409, 834, 2916, 3761
BslI CCNNNNNNNGG 5 cut(s) 86, 1019, 1426, 1856, 2565
BsmAI GTCTC 5 cut(s) 1471, 1629, 1914, 2257, 3717
BsmBI CGTCTC 1 cut(s) 3717
BsmI GAATGC 2 cut(s) 986, 1924
BsnI GGCC 3 cut(s) 807, 3471, 3783
BsoBI CYCGRG 1 cut(s) 318
Bsp119I TTCGAA 2 cut(s) 110, 2391
Bsp1286I GDGCHC 2 cut(s) 2310, 2581
Bsp1407I TGTACA 1 cut(s) 1879
Bsp1720I GCTNAGC 1 cut(s) 1626
Bsp19I CCATGG 2 cut(s) 174, 2768
BspACI CCGC 7 cut(s) 152, 282, 808, 1780, 1783, 3673, 3784
BspANI GGCC 3 cut(s) 807, 3471, 3783
BspCNI CTCAG 9 cut(s) 718, 1467, 1618, 1924, 2005, 2458, 2502, 2581, 3253
BspFNI CGCG 2 cut(s) 282, 810
BspHI TCATGA 1 cut(s) 2047
BspLI GGNNCC 4 cut(s) 150, 202, 363, 3491
BspMAI CTGCAG 3 cut(s) 103, 2136, 2986
BspMI ACCTGC 2 cut(s) 1682, 2423
BspPI GGATC 9 cut(s) 356, 369, 1010, 1074, 1104, 1172, 1523, 3354, 3632
BspQI GCTCTTC 2 cut(s) 1755, 3388
BspT104I TTCGAA 2 cut(s) 110, 2391
BspT107I GGYRCC 2 cut(s) 148, 200
BspTI CTTAAG 1 cut(s) 2438
BsrDI GCAATG 4 cut(s) 64, 420, 1120, 1274
BsrFI RCCGGY 3 cut(s) 833, 2915, 3760
BsrGI TGTACA 1 cut(s) 1879
BsrI ACTGG 4 cut(s) 1003, 1233, 1334, 1598
BssAI RCCGGY 3 cut(s) 833, 2915, 3760
BssECI CCNNGG 6 cut(s) 161, 174, 788, 1547, 2004, 2768
BssNI GRCGYC 1 cut(s) 1770
BssSI CACGAG 3 cut(s) 1377, 2973, 3182
BssT1I CCWWGG 4 cut(s) 161, 174, 2004, 2768
Bst2BI CACGAG 3 cut(s) 1377, 2973, 3182
Bst2UI CCWGG 4 cut(s) 804, 1020, 1548, 2961
Bst4CI ACNGT 8 cut(s) 131, 423, 910, 951, 2945, 3148, 3163, 3483
Bst6I CTCTTC 5 cut(s) 1243, 1755, 1899, 3319, 3388
BstACI GRCGYC 1 cut(s) 1770
BstAFI CTTAAG 1 cut(s) 2438
BstAPI GCANNNNNTGC 1 cut(s) 194
BstAUI TGTACA 1 cut(s) 1879
BstBI TTCGAA 2 cut(s) 110, 2391
BstC8I GCNNGC 3 cut(s) 481, 1127, 3712
BstDSI CCRYGG 2 cut(s) 174, 2768
BstFNI CGCG 2 cut(s) 282, 810
BstH2I RGCGCY 2 cut(s) 2090, 2603
BstHHI GCGC 5 cut(s) 1264, 1308, 2089, 2367, 2602
BstMAI GTCTC 5 cut(s) 1471, 1629, 1914, 2257, 3717
BstMCI CGRYCG 2 cut(s) 838, 3761
BstMWI GCNNNNNNNGC 9 cut(s) 101, 194, 541, 1126, 1802, 1951, 2093, 2585, 3387
BstNI CCWGG 4 cut(s) 804, 1020, 1548, 2961
BstNSI RCATGY 2 cut(s) 3049, 3230
BstSCI CCNGG 6 cut(s) 183, 408, 802, 1018, 1546, 2959
BstSFI CTRYAG 4 cut(s) 99, 2132, 2646, 2982
BstUI CGCG 2 cut(s) 282, 810
BstV2I GAAGAC 3 cut(s) 1495, 2214, 2668
BstX2I RGATCY 5 cut(s) 361, 1066, 1177, 1828, 3346
BstYI RGATCY 5 cut(s) 361, 1066, 1177, 1828, 3346
BsuI GTATCC 1 cut(s) 2842
BsuRI GGCC 3 cut(s) 807, 3471, 3783
BtgI CCRYGG 2 cut(s) 174, 2768
BtrI CACGTC 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 2099
BtsIMutI CAGTG 5 cut(s) 136, 915, 1323, 1516, 2099
BveI ACCTGC 2 cut(s) 1682, 2423
Cac8I GCNNGC 3 cut(s) 481, 1127, 3712
CaiI CAGNNNCTG 1 cut(s) 914
CciI TCATGA 1 cut(s) 2047
CfoI GCGC 5 cut(s) 1264, 1308, 2089, 2367, 2602
Cfr10I RCCGGY 3 cut(s) 833, 2915, 3760
Cfr13I GGNCC 3 cut(s) 165, 308, 785
CseI GACGC 1 cut(s) 3550
CspAI ACCGGT 3 cut(s) 833, 2915, 3760
CspCI CAANNNNNGTGG 2 cut(s) 891, 926
DraI TTTAAA 1 cut(s) 2995
DraIII CACNNNGTG 2 cut(s) 710, 3527
EaeI YGGCCR 2 cut(s) 3469, 3781
Eam1104I CTCTTC 5 cut(s) 1243, 1755, 1899, 3319, 3388
EarI CTCTTC 5 cut(s) 1243, 1755, 1899, 3319, 3388
Ecl136II GAGCTC 1 cut(s) 2579
Eco130I CCWWGG 4 cut(s) 161, 174, 2004, 2768
Eco24I GRGCYC 2 cut(s) 2310, 2581
Eco47I GGWCC 3 cut(s) 165, 308, 785
Eco47III AGCGCT 1 cut(s) 2601
Eco53kI GAGCTC 1 cut(s) 2579
Eco57I CTGAAG 4 cut(s) 1492, 2075, 3369, 3698
Eco88I CYCGRG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 2579
EcoO109I RGGNCCY 1 cut(s) 165
EcoRI GAATTC 3 cut(s) 1079, 1333, 1495
EcoRII CCWGG 4 cut(s) 802, 1018, 1546, 2959
EcoT14I CCWWGG 4 cut(s) 161, 174, 2004, 2768
EcoT22I ATGCAT 1 cut(s) 3613
EcoT38I GRGCYC 2 cut(s) 2310, 2581
ErhI CCWWGG 4 cut(s) 161, 174, 2004, 2768
Esp3I CGTCTC 1 cut(s) 3717
FalI AAGNNNNNCTT 4 cut(s) 2604, 2636, 3054, 3086
FauNDI CATATG 2 cut(s) 892, 2796
FbaI TGATCA 3 cut(s) 609, 1924, 2044
FblI GTMKAC 1 cut(s) 3075
FriOI GRGCYC 2 cut(s) 2310, 2581
FspBI CTAG 6 cut(s) 54, 740, 963, 1809, 2105, 2585
FspI TGCGCA 1 cut(s) 1263
GlaI GCGC 5 cut(s) 1263, 1307, 2088, 2366, 2601
GsuI CTGGAG 2 cut(s) 124, 459
HaeII RGCGCY 2 cut(s) 2090, 2603
HaeIII GGCC 3 cut(s) 807, 3471, 3783
HapII CCGG 5 cut(s) 185, 409, 834, 2916, 3761
HgaI GACGC 1 cut(s) 3550
HhaI GCGC 5 cut(s) 1264, 1308, 2089, 2367, 2602
Hin1I GRCGYC 1 cut(s) 1770
Hin6I GCGC 5 cut(s) 1262, 1306, 2087, 2365, 2600
HinP1I GCGC 5 cut(s) 1262, 1306, 2087, 2365, 2600
HincII GTYRAC 4 cut(s) 1291, 2419, 3395, 3501
HindII GTYRAC 4 cut(s) 1291, 2419, 3395, 3501
HindIII AAGCTT 4 cut(s) 481, 2116, 2378, 2405
HpaI GTTAAC 1 cut(s) 3501
HpaII CCGG 5 cut(s) 185, 409, 834, 2916, 3761
HphI GGTGA 6 cut(s) 1002, 1742, 1871, 2240, 3775, 3788
Hpy99I CGWCG 1 cut(s) 3731
HpyAV CCTTC 7 cut(s) 227, 772, 2405, 2767, 3089, 3216, 3314
HpyCH4III ACNGT 8 cut(s) 131, 423, 910, 951, 2945, 3148, 3163, 3483
HpyCH4IV ACGT 7 cut(s) 399, 493, 1203, 1414, 1770, 1876, 3651
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 194, 541, 1126, 1802, 1951, 2093, 2585, 3387
HpySE526I ACGT 7 cut(s) 399, 493, 1203, 1414, 1770, 1876, 3651
Hsp92I GRCGYC 1 cut(s) 1770
HspAI GCGC 5 cut(s) 1262, 1306, 2087, 2365, 2600
Ksp22I TGATCA 3 cut(s) 609, 1924, 2044
KspAI GTTAAC 1 cut(s) 3501
LguI GCTCTTC 2 cut(s) 1755, 3388
LmnI GCTCC 3 cut(s) 143, 2290, 2576
MaeI CTAG 6 cut(s) 54, 740, 963, 1809, 2105, 2585
MaeII ACGT 7 cut(s) 399, 493, 1203, 1414, 1770, 1876, 3651
MfeI CAATTG 6 cut(s) 6, 852, 1821, 1928, 2757, 2874
MflI RGATCY 5 cut(s) 361, 1066, 1177, 1828, 3346
MhlI GDGCHC 2 cut(s) 2310, 2581
MlsI TGGCCA 1 cut(s) 3471
MluNI TGGCCA 1 cut(s) 3471
MlyI GAGTC 7 cut(s) 716, 744, 1916, 2424, 2682, 3512, 3596
MmeI TCCRAC 5 cut(s) 487, 1140, 1225, 3030, 3301
Mox20I TGGCCA 1 cut(s) 3471
Mph1103I ATGCAT 1 cut(s) 3613
MroXI GAANNNNTTC 2 cut(s) 422, 3744
MscI TGGCCA 1 cut(s) 3471
MslI CAYNNNNRTG 2 cut(s) 1764, 2799
Msp20I TGGCCA 1 cut(s) 3471
MspA1I CMGCKG 1 cut(s) 1625
MspCI CTTAAG 1 cut(s) 2438
MspI CCGG 5 cut(s) 185, 409, 834, 2916, 3761
MspR9I CCNGG 6 cut(s) 185, 410, 804, 1020, 1548, 2961
MunI CAATTG 6 cut(s) 6, 852, 1821, 1928, 2757, 2874
Mva1269I GAATGC 2 cut(s) 986, 1924
MvaI CCWGG 4 cut(s) 804, 1020, 1548, 2961
MvnI CGCG 2 cut(s) 282, 810
MwoI GCNNNNNNNGC 9 cut(s) 101, 194, 541, 1126, 1802, 1951, 2093, 2585, 3387
NciI CCSGG 2 cut(s) 185, 410
NcoI CCATGG 2 cut(s) 174, 2768
NdeI CATATG 2 cut(s) 892, 2796
NlaIV GGNNCC 4 cut(s) 150, 202, 363, 3491
NmuCI GTSAC 5 cut(s) 1171, 2069, 3142, 3423, 3515
NsbI TGCGCA 1 cut(s) 1263
NsiI ATGCAT 1 cut(s) 3613
NspI RCATGY 2 cut(s) 3049, 3230
NspV TTCGAA 2 cut(s) 110, 2391
PagI TCATGA 1 cut(s) 2047
PaqCI CACCTGC 1 cut(s) 1682
PciI ACATGT 1 cut(s) 3045
PciSI GCTCTTC 2 cut(s) 1755, 3388
PcsI WCGNNNNNNNCGW 1 cut(s) 1384
PctI GAATGC 2 cut(s) 986, 1924
PdmI GAANNNNTTC 2 cut(s) 422, 3744
PfeI GAWTC 8 cut(s) 71, 262, 285, 455, 1316, 2336, 3128, 3572
PflMI CCANNNNNTGG 1 cut(s) 1426
PfoI TCCNGGA 2 cut(s) 1018, 2959
PinAI ACCGGT 3 cut(s) 833, 2915, 3760
PleI GAGTC 7 cut(s) 716, 743, 1915, 2423, 2681, 3512, 3595
PpsI GAGTC 7 cut(s) 716, 743, 1915, 2423, 2681, 3512, 3595
PpuMI RGGWCCY 1 cut(s) 165
PscI ACATGT 1 cut(s) 3045
PshBI ATTAAT 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 2581
Psp1406I AACGTT 1 cut(s) 3651
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 4 cut(s) 802, 1018, 1546, 2959
PspGI CCWGG 4 cut(s) 802, 1018, 1546, 2959
PspN4I GGNNCC 4 cut(s) 150, 202, 363, 3491
PspPI GGNCC 3 cut(s) 165, 308, 785
PspPPI RGGWCCY 1 cut(s) 165
PsrI GAACNNNNNNTAC 2 cut(s) 1338, 1370
PstI CTGCAG 3 cut(s) 103, 2136, 2986
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 5 cut(s) 361, 1066, 1177, 1828, 3346
PvuII CAGCTG 1 cut(s) 1625
RseI CAYNNNNRTG 2 cut(s) 1764, 2799
SacI GAGCTC 1 cut(s) 2581
SapI GCTCTTC 2 cut(s) 1755, 3388
Sau96I GGNCC 3 cut(s) 165, 308, 785
ScaI AGTACT 1 cut(s) 1473
SchI GAGTC 7 cut(s) 716, 744, 1916, 2424, 2682, 3512, 3596
ScrFI CCNGG 6 cut(s) 185, 410, 804, 1020, 1548, 2961
SduI GDGCHC 2 cut(s) 2310, 2581
SfcI CTRYAG 4 cut(s) 99, 2132, 2646, 2982
SfuI TTCGAA 2 cut(s) 110, 2391
SinI GGWCC 3 cut(s) 165, 308, 785
SmiMI CAYNNNNRTG 2 cut(s) 1764, 2799
SmlI CTYRAG 2 cut(s) 2438, 2563
SmoI CTYRAG 2 cut(s) 2438, 2563
SsiI CCGC 7 cut(s) 152, 282, 808, 1780, 1783, 3673, 3784
SspI AATATT 2 cut(s) 605, 3000
SspMI CTAG 6 cut(s) 54, 740, 963, 1809, 2105, 2585
SstI GAGCTC 1 cut(s) 2581
StyD4I CCNGG 6 cut(s) 183, 408, 802, 1018, 1546, 2959
StyI CCWWGG 4 cut(s) 161, 174, 2004, 2768
TaaI ACNGT 8 cut(s) 131, 423, 910, 951, 2945, 3148, 3163, 3483
TaiI ACGT 7 cut(s) 402, 496, 1206, 1417, 1773, 1879, 3654
TaqI TCGA 8 cut(s) 110, 466, 733, 838, 1099, 2205, 2310, 2391
TatI WGTACW 4 cut(s) 441, 1056, 1471, 1879
TauI GCSGC 4 cut(s) 810, 1783, 3676, 3786
TfiI GAWTC 8 cut(s) 71, 262, 285, 455, 1316, 2336, 3128, 3572
TscAI CASTG 5 cut(s) 136, 915, 1330, 1516, 2106
TseFI GTSAC 5 cut(s) 1171, 2069, 3142, 3423, 3515
Tsp45I GTSAC 5 cut(s) 1171, 2069, 3142, 3423, 3515
TspGWI ACGGA 2 cut(s) 292, 1583
TspRI CASTG 5 cut(s) 136, 915, 1330, 1516, 2106
Van91I CCANNNNNTGG 1 cut(s) 1426
Vha464I CTTAAG 1 cut(s) 2438
VpaK11BI GGWCC 3 cut(s) 165, 308, 785
VspI ATTAAT 1 cut(s) 626
XapI RAATTY 8 cut(s) 567, 1079, 1333, 1495, 1737, 2351, 3020, 3744
XceI RCATGY 2 cut(s) 3049, 3230
XmiI GTMKAC 1 cut(s) 3075
XmnI GAANNNNTTC 2 cut(s) 422, 3744
XspI CTAG 6 cut(s) 54, 740, 963, 1809, 2105, 2585
ZraI GACGTC 1 cut(s) 1771
ZrmI AGTACT 1 cut(s) 1473
Zsp2I ATGCAT 1 cut(s) 3613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.