Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014218

Physical Location & Seq

Genomic Coordinates Reverse (-)

9260352 .. 9269213

Gene Structure 8862 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1113900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGTTGCAGAACAAGTCATTCGTCAAGAGAACCAGAGGCGGCAAAATCACCAAGGTCGTCAGAGAGCACTATCTCCGGCACGACATATACTGCGGAGCTCCGGTATGCAAAGTTTGCGATACGTCCGGAGCTCGATTGAGCCCTACCGCTTCCACCATTCTCATCTTTGATACCAATGTCGTCCTTCGTCAGATTGATTTGATTGAGAATCCGGCGATCGAGGATGTGGTTGTTCTTTCGATTGTGCTGGAAGAGGTTAAAAACAGGAACTTGTCTGTTTACAATAGAGTTAGAGCTCTGTGCAGCAATTCTCTGAGGAATTTTTTCGTCTTTTCGAATGAACATCACAAGGATACATATGTTACGGACATGAGCGGAGAAAGTAAAAATGATCGAAATGATAGAGCAATTCGGGTGGCTGCTCAGTGGTATCAAAGTCATCTTGCTGGCTCAGCTAAGGTTTTACTTATAACCAATGATAAGGAGAATAAGAGGAAGGCTATTGAAGAGGGAATTTCTGCAGAGACAGTTGAATCGTATGTGAGGTCATTGAGTCGGCCAGATTTGCTTGACCTGCTCGTGCAGCCTGCATCAGAAGATGTAAAGATGGAGGACGTTGAAGATTTGAGACCATCGAAAAGGAAAGTCGTCTACTCAGAGCATAAGCCCATGTCTGAAATTACTTCCGGTTTGCATTGTGGCATATACCATCAAGGAAAACTTCGAGTTAATCGTTACAACCCATTTGAAGCATATGTTGGAAGTGAGAGCATTGGTGATGAAATTATAATCTATGGACGTACAAACATGAATAGGGCTTTTGATGGTGATATAGTGGCAGTTGAGCTTCTGCCTCAAGATCAATGGCATGAGGAAAAATCTATAGCACTAGCAGATGAAGAGGATGAGCAAGAAGAAGATGTCCATCTGGTTCCAGGCAGTGCTGATGATGCTCCTAGGACTACAGCTCCACATGGTTCTGCCGGAGTAACAGAATCTGTCCCAAATCGACCATCTGGCCGTGTCATTGGTATTATAAAGCGGAATTGGCATTCTTATTGTGGATCTTTGGAGCCCATGGCTAAGCCTGCAGGTGGTGGTGGTATTGCCCATGCTTTATTTGTCTCAAAAGATCGTAGGATTCCGAAGATTCGAATTCAAACTCGACAGCTTGAGAATCTGCTGGACAAGAGAATTACCGTGGCAGTTGATTCATGGGACCCTTTGTCTCGATATCCTTCTGGCCATTATGTACGAACCATAGGAGTAATAGGTGATAAAGATACTGAAACTGAGGTGGTCTTGATTGAGAATGATATAAATACAAGACCATTTTCTTCTCAAGTTCTAGCATGCCTGCCGCCGCTACCATGGTTTGTGTCTTCTGAAGATTTGGCAAATCCTATTAGACAGGATTTGCGACAATTGCGTGTCTTTAGTGTTGATCCTCCTGGATGCAAGGATATAGATGATGCATTGCACTGTACAGCTCTTCCAAATGGAAATTACGAAGTTGGAGTCCATATTGCTGATGTAACAAATTTTGTACACTCGGGAACTCCTCTTGATGATGAGGCTTCACAAAGAGGCACATCGGTCTACCTTGTTGAACGGCGCATTGACATGCTTCCAAAACCTCTGACAGAAGATATATGTTCTCTTCGAGCTGATGTGGAAAGGCTAGCCTTTTCTGTCATTTGGGAAATGACAGCTGAAGCTGAGATTATTTCTGCAAGATACACCAAAAGTGTTATTAAATCGTGTGCAGCATTATCTTACATTGAAGCTCAAGCGAGGATGGATGATAGTCGGTTGATGGATCCATTAACTACAGATTTAAGAAACATGAACTCTTTAGCAAAGATAATGAGGGCAAGGCGTATTGAGAGAGGAGCCTTAACTCTTGCATCTGCTGAAGTCAAATTTGAAATTGACACTGAGACTCATGATCCTCTTGATATCGGTATGTACCAGATACGTGAAGCAAACCAAATGGTGGAGGAGTTTATGCTTGCCGCTAATGTTTCAGTTGCTGAGAAGATTCTTAAACATTTCCCTCTATGTTCACTATTGAGGCGCCACCCTACTCCGACCAGAGAGATGCTTGAACCGCTGTTGCGTACAGCTGCTGCTATTGGTCTCAACTTGGATGTCTCATCATCAAAAGCACTAGCTGATTCACTTGACTCTGCTGTGGGTGATGATCCGTACTTCAACAAGCTTATTCGTATACTGGCTACGAGATGCATGACCCAGGCTGTTTACTTCTGTAGTGGGGATCTCAGCCCTCCGGAATATCTTCATTATGGGCTTGCAGCGCTTCTATACACTCATTTCACCTCTCCTATTAGGAGATATGCAGATGTCATAGTACACAGGTTGCTTGCTGCATCTCTGGGGATATACAAGCTTCCAACTGTATTCCAAGACGGACCCCGACTCACAAGCATTGCTGACAATTTAAATTATCGGCATAGGAATGCCCAGATGGCAAGTCGGGCCTCAGTGGAGCTCCATACTCTCATTTTTTTCAAAAAACGGCCAACGGACACAGAGGCAAGAATAGTCAGAATAAGATCCAATGGATTTTTCGTATTTGTGCCCAAGTACGGCATAGAAGGGCCGGTGTACTTGACACCAAGGGGTGAGAAGGGAGGCGGAGAATGGTATGTGGATGAGCAACAACAGAAGATTAGAAAAATGGACGGTAGCATTTCATACAGCGTTCTGCAGACAGTGTTTATTCATATGGAGATTGTGGAACCCCAGCCCAACAGGCCAAAACTCCAACTATCGCTAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000176 GO:0000178 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0004532 GO:0004540 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0019899 GO:0022613 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0051020 GO:0065007 GO:0065009 GO:0070013 GO:0071034 GO:0071043 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1905354

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PIN_4 RNB Rrp44_CSD1 Rrp44_S1

Protein Analysis

935

Amino Acids

104.91

Weight (kDa)

6.15

Isoelectric Point (pI)

45.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIN_4	PF13638	54 - 177	1.6e-20	PIN domain
Rrp44_CSD1	PF17216	218 - 318	1.7e-14	Rrp44-like cold shock domain
OB_HELZ2	PF25049	234 - 292	3.4e-06	3'-5' exoribonuclease HELZ2, OB-fold domain
OB_Dis3	PF17849	372 - 439	4.5e-20	Dis3-like cold-shock domain 2 (CSD2)
RNB	PF00773	470 - 799	1.2e-99	RNB domain
Rrp44_S1	PF17215	851 - 895	1.5e-06	S1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17510 AT2G17510
fragaria_vesca	FvH4_1g10170 FvH4_1g10170
malus_domestica	MD02G1113900.v1.1 MD15G1229000.v1.1
prunus_persica	Prupe.7G185100_v2.0.a1
pyrus_communis	pycom02g08870 pycom15g20420
rosa_chinensis	RchiOBHm_Chr2g0096961
rosa_laevigata	RLG00000016687
rosa_multiflora	Rmu_sc0001651.1_g000008
rosa_roxburghii	Rroxscaffold_2G00145120
rosa_rugosa	Rorug02G0062300
rosa_samantha	Rh2AG110300 Rh2BG112100
rosa_wichuraiana	Rw2G008530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	470, 788, 1037
AarI	CACCTGC	1 cut(s)	1082
Acc36I	ACCTGC	2 cut(s)	582, 1082
AccB1I	GGYRCC	1 cut(s)	2076
AccB7I	CCANNNNNTGG	1 cut(s)	1996
AccBSI	CCGCTC	1 cut(s)	375
AccI	GTMKAC	3 cut(s)	651, 1599, 2230
AccIII	TCCGGA	2 cut(s)	125, 2290
AclWI	GGATC	8 cut(s)	1072, 1439, 1814, 1827, 1943, 2198, 2286, 2571
AcoI	YGGCCR	4 cut(s)	557, 1018, 1243, 2540
AcsI	RAATTY	5 cut(s)	319, 513, 1155, 1540, 1922
AcuI	CTGAAG	3 cut(s)	1407, 1734, 1935
AcyI	GRCGYC	1 cut(s)	2077
AfeI	AGCGCT	1 cut(s)	2319
AfiI	CCNNNNNNNGG	3 cut(s)	1094, 1996, 2609
AhdI	GACNNNNNGTC	1 cut(s)	1225
AjnI	CCWGG	3 cut(s)	934, 1450, 2253
AjuI	GAANNNNNNNTTGG	4 cut(s)	741, 773, 2573, 2605
Alw21I	GWGCWC	5 cut(s)	69, 100, 133, 298, 2514
Alw26I	GTCTC	7 cut(s)	518, 622, 1129, 1233, 1934, 2144, 2158
AlwI	GGATC	8 cut(s)	1072, 1439, 1814, 1827, 1943, 2198, 2286, 2571
AlwNI	CAGNNNCTG	3 cut(s)	998, 2033, 2129
Ama87I	CYCGRG	1 cut(s)	1552
Aor13HI	TCCGGA	2 cut(s)	125, 2290
Aor51HI	AGCGCT	1 cut(s)	2319
AoxI	GGCC	7 cut(s)	557, 1018, 1243, 2499, 2540, 2621, 2777
ApeKI	GCWGC	8 cut(s)	303, 419, 583, 1766, 2126, 2129, 2315, 2387
ApoI	RAATTY	5 cut(s)	319, 513, 1155, 1540, 1922
ArsI	GACNNNNNNTTYG	2 cut(s)	1503, 1535
Asp700I	GAANNNNTTC	2 cut(s)	323, 2298
AspA2I	CCTAGG	1 cut(s)	956
AspLEI	GCGC	3 cut(s)	1617, 2079, 2320
AspS9I	GGNCC	4 cut(s)	1219, 2432, 2499, 2621
AsuHPI	GGTGA	7 cut(s)	40, 788, 839, 1286, 2210, 2329, 2657
AsuII	TTCGAA	2 cut(s)	335, 1153
AsuNHI	GCTAGC	1 cut(s)	1681
AvaI	CYCGRG	1 cut(s)	1552
AvaII	GGWCC	2 cut(s)	1219, 2432
AvrII	CCTAGG	1 cut(s)	956
BaeGI	GKGCMC	1 cut(s)	2604
BaeI	ACNNNNGTAYC	4 cut(s)	162, 195, 345, 378
BalI	TGGCCA	1 cut(s)	1245
BamHI	GGATCC	1 cut(s)	1819
BanI	GGYRCC	1 cut(s)	2076
BanII	GRGCYC	6 cut(s)	100, 133, 143, 298, 1077, 2514
BauI	CACGAG	1 cut(s)	578
BbsI	GAAGAC	1 cut(s)	1374
Bbv12I	GWGCWC	5 cut(s)	69, 100, 133, 298, 2514
BbvI	GCAGC	8 cut(s)	315, 406, 595, 1778, 2113, 2116, 2327, 2374
BccI	CCATC	9 cut(s)	601, 640, 717, 818, 933, 1021, 1792, 1810, 2482
BceAI	ACGGC	4 cut(s)	1005, 1628, 2555, 2626
BciT130I	CCWGG	3 cut(s)	936, 1452, 2255
BciVI	GTATCC	1 cut(s)	346
BcoDI	GTCTC	7 cut(s)	518, 622, 1129, 1233, 1934, 2144, 2158
BfaI	CTAG	6 cut(s)	890, 957, 1349, 1682, 2171, 2798
BfmI	CTRYAG	7 cut(s)	519, 882, 963, 1089, 1830, 2269, 2729
BfoI	RGCGCY	2 cut(s)	2080, 2321
BfuAI	ACCTGC	2 cut(s)	582, 1082
BfuI	GTATCC	1 cut(s)	346
BglI	GCCNNNNNGGC	2 cut(s)	2489, 2776
BlnI	CCTAGG	1 cut(s)	956
BlpI	GCTNAGC	2 cut(s)	451, 1083
Bme1390I	CCNGG	3 cut(s)	936, 1452, 2255
Bme18I	GGWCC	2 cut(s)	1219, 2432
BmeRI	GACNNNNNGTC	1 cut(s)	1225
BmeT110I	CYCGRG	1 cut(s)	1552
BmgT120I	GGNCC	4 cut(s)	1219, 2432, 2499, 2621
BmiI	GGNNCC	9 cut(s)	933, 1074, 1220, 1221, 1821, 1894, 2078, 2434, 2763
BmrFI	CCNGG	3 cut(s)	936, 1452, 2255
BmsI	GCATC	8 cut(s)	599, 940, 1445, 1462, 1916, 2091, 2234, 2399
BmtI	GCTAGC	1 cut(s)	1685
BpiI	GAAGAC	1 cut(s)	1374
Bpu10I	CCTNAGC	1 cut(s)	456
Bpu1102I	GCTNAGC	2 cut(s)	451, 1083
Bpu14I	TTCGAA	2 cut(s)	335, 1153
BpuEI	CTTGAG	4 cut(s)	840, 1193, 1326, 1773
BsaAI	YACGTR	1 cut(s)	1979
BsaBI	GATNNNNATC	1 cut(s)	924
BsaHI	GRCGYC	1 cut(s)	2077
BsaI	GGTCTC	2 cut(s)	622, 2144
BsaJI	CCNNGG	7 cut(s)	51, 956, 1077, 1200, 1370, 2253, 2639
BsaWI	WCCGGW	4 cut(s)	100, 125, 686, 2290
BsaXI	ACNNNNNCTCC	4 cut(s)	87, 117, 952, 982
Bsc4I	CCNNNNNNNGG	3 cut(s)	1094, 1996, 2609
Bse118I	RCCGGY	1 cut(s)	2623
Bse1I	ACTGG	1 cut(s)	2238
Bse3DI	GCAATG	2 cut(s)	1475, 2448
Bse8I	GATNNNNATC	1 cut(s)	924
BseAI	TCCGGA	2 cut(s)	125, 2290
BseBI	CCWGG	3 cut(s)	936, 1452, 2255
BseDI	CCNNGG	7 cut(s)	51, 956, 1077, 1200, 1370, 2253, 2639
BseGI	GGATG	7 cut(s)	229, 910, 1460, 1803, 1807, 2155, 2680
BseJI	GATNNNNATC	1 cut(s)	924
BseLI	CCNNNNNNNGG	3 cut(s)	1094, 1996, 2609
BseMI	GCAATG	2 cut(s)	1475, 2448
BseNI	ACTGG	1 cut(s)	2238
BseRI	GAGGAG	3 cut(s)	1551, 1905, 2015
BseSI	GKGCMC	1 cut(s)	2604
BseXI	GCAGC	8 cut(s)	315, 406, 595, 1778, 2113, 2116, 2327, 2374
BseYI	CCCAGC	1 cut(s)	2766
BsgI	GTGCAG	3 cut(s)	322, 602, 1785
Bsh1285I	CGRYCG	1 cut(s)	219
BshFI	GGCC	7 cut(s)	559, 1020, 1245, 2501, 2542, 2623, 2779
BshNI	GGYRCC	1 cut(s)	2076
BsiEI	CGRYCG	1 cut(s)	219
BsiHKAI	GWGCWC	5 cut(s)	69, 100, 133, 298, 2514
BsiHKCI	CYCGRG	1 cut(s)	1552
BsiSI	CCGG	8 cut(s)	76, 101, 126, 212, 687, 984, 2291, 2624
BslFI	GGGAC	2 cut(s)	986, 1232
BslI	CCNNNNNNNGG	3 cut(s)	1094, 1996, 2609
BsmAI	GTCTC	7 cut(s)	518, 622, 1129, 1233, 1934, 2144, 2158
BsmFI	GGGAC	2 cut(s)	986, 1232
BsmI	GAATGC	2 cut(s)	1051, 2485
BsnI	GGCC	7 cut(s)	559, 1020, 1245, 2501, 2542, 2623, 2779
Bso31I	GGTCTC	2 cut(s)	622, 2144
BsoBI	CYCGRG	1 cut(s)	1552
Bsp119I	TTCGAA	2 cut(s)	335, 1153
Bsp1286I	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1077, 2514, 2604
Bsp13I	TCCGGA	2 cut(s)	125, 2290
Bsp1407I	TGTACA	2 cut(s)	1484, 1546
Bsp1720I	GCTNAGC	2 cut(s)	451, 1083
Bsp19I	CCATGG	2 cut(s)	1077, 1370
BspANI	GGCC	7 cut(s)	559, 1020, 1245, 2501, 2542, 2623, 2779
BspEI	TCCGGA	2 cut(s)	125, 2290
BspHI	TCATGA	1 cut(s)	1945
BspLI	GGNNCC	9 cut(s)	933, 1074, 1220, 1221, 1821, 1894, 2078, 2434, 2763
BspMAI	CTGCAG	3 cut(s)	523, 1093, 2733
BspMI	ACCTGC	2 cut(s)	582, 1082
BspOI	GCTAGC	1 cut(s)	1685
BspPI	GGATC	8 cut(s)	1072, 1439, 1814, 1827, 1943, 2198, 2286, 2571
BspQI	GCTCTTC	1 cut(s)	1497
BspT104I	TTCGAA	2 cut(s)	335, 1153
BspT107I	GGYRCC	1 cut(s)	2076
BspTNI	GGTCTC	2 cut(s)	622, 2144
BsrBI	CCGCTC	1 cut(s)	375
BsrDI	GCAATG	2 cut(s)	1475, 2448
BsrFI	RCCGGY	1 cut(s)	2623
BsrGI	TGTACA	2 cut(s)	1484, 1546
BsrI	ACTGG	1 cut(s)	2238
BssAI	RCCGGY	1 cut(s)	2623
BssECI	CCNNGG	7 cut(s)	51, 956, 1077, 1200, 1370, 2253, 2639
BssNAI	GTATAC	1 cut(s)	2231
BssNI	GRCGYC	1 cut(s)	2077
BssSI	CACGAG	1 cut(s)	578
BssT1I	CCWWGG	5 cut(s)	51, 956, 1077, 1370, 2639
Bst1107I	GTATAC	1 cut(s)	2231
Bst2BI	CACGAG	1 cut(s)	578
Bst2UI	CCWGG	3 cut(s)	936, 1452, 2255
Bst4CI	ACNGT	6 cut(s)	529, 1201, 1484, 2419, 2708, 2737
Bst6I	CTCTTC	5 cut(s)	246, 501, 894, 1497, 1665
BstACI	GRCGYC	1 cut(s)	2077
BstAPI	GCANNNNNTGC	1 cut(s)	114
BstAUI	TGTACA	2 cut(s)	1484, 1546
BstBAI	YACGTR	1 cut(s)	1979
BstBI	TTCGAA	2 cut(s)	335, 1153
BstC8I	GCNNGC	9 cut(s)	448, 588, 1089, 1354, 1358, 1683, 2013, 2313, 2385
BstDSI	CCRYGG	3 cut(s)	1077, 1200, 1370
BstF5I	GGATG	7 cut(s)	229, 910, 1460, 1803, 1807, 2155, 2680
BstH2I	RGCGCY	2 cut(s)	2080, 2321
BstHHI	GCGC	3 cut(s)	1617, 2079, 2320
BstMAI	GTCTC	7 cut(s)	518, 622, 1129, 1233, 1934, 2144, 2158
BstMCI	CGRYCG	1 cut(s)	219
BstNI	CCWGG	3 cut(s)	936, 1452, 2255
BstNSI	RCATGY	2 cut(s)	1356, 1627
BstSCI	CCNGG	3 cut(s)	934, 1450, 2253
BstSFI	CTRYAG	7 cut(s)	519, 882, 963, 1089, 1830, 2269, 2729
BstSLI	GKGCMC	1 cut(s)	2604
BstV1I	GCAGC	8 cut(s)	315, 406, 595, 1778, 2113, 2116, 2327, 2374
BstV2I	GAAGAC	1 cut(s)	1374
BstX2I	RGATCY	4 cut(s)	1064, 1819, 2278, 2576
BstYI	RGATCY	4 cut(s)	1064, 1819, 2278, 2576
BstZ17I	GTATAC	1 cut(s)	2231
BsuI	GTATCC	1 cut(s)	346
BsuRI	GGCC	7 cut(s)	559, 1020, 1245, 2501, 2542, 2623, 2779
BtgI	CCRYGG	3 cut(s)	1077, 1200, 1370
BtsCI	GGATG	7 cut(s)	229, 910, 1460, 1803, 1807, 2155, 2680
BtsI	GCAGTG	1 cut(s)	946
BtsIMutI	CAGTG	6 cut(s)	431, 946, 1480, 1935, 2511, 2742
BveI	ACCTGC	2 cut(s)	582, 1082
Cac8I	GCNNGC	9 cut(s)	448, 588, 1089, 1354, 1358, 1683, 2013, 2313, 2385
CaiI	CAGNNNCTG	3 cut(s)	998, 2033, 2129
CciI	TCATGA	1 cut(s)	1945
CfoI	GCGC	3 cut(s)	1617, 2079, 2320
Cfr10I	RCCGGY	1 cut(s)	2623
Cfr13I	GGNCC	4 cut(s)	1219, 2432, 2499, 2621
CspCI	CAANNNNNGTGG	2 cut(s)	396, 431
DinI	GGCGCC	1 cut(s)	2078
DraI	TTTAAA	1 cut(s)	2463
DriI	GACNNNNNGTC	1 cut(s)	1225
EaeI	YGGCCR	4 cut(s)	557, 1018, 1243, 2540
Eam1104I	CTCTTC	5 cut(s)	246, 501, 894, 1497, 1665
Eam1105I	GACNNNNNGTC	1 cut(s)	1225
EarI	CTCTTC	5 cut(s)	246, 501, 894, 1497, 1665
EciI	GGCGGA	1 cut(s)	2673
Ecl136II	GAGCTC	4 cut(s)	98, 131, 296, 2512
Eco130I	CCWWGG	5 cut(s)	51, 956, 1077, 1370, 2639
Eco24I	GRGCYC	6 cut(s)	100, 133, 143, 298, 1077, 2514
Eco31I	GGTCTC	2 cut(s)	622, 2144
Eco32I	GATATC	2 cut(s)	1235, 1960
Eco47I	GGWCC	2 cut(s)	1219, 2432
Eco47III	AGCGCT	1 cut(s)	2319
Eco53kI	GAGCTC	4 cut(s)	98, 131, 296, 2512
Eco57I	CTGAAG	3 cut(s)	1407, 1734, 1935
Eco88I	CYCGRG	1 cut(s)	1552
EcoICRI	GAGCTC	4 cut(s)	98, 131, 296, 2512
EcoO109I	RGGNCCY	1 cut(s)	1219
EcoRI	GAATTC	1 cut(s)	1155
EcoRII	CCWGG	3 cut(s)	934, 1450, 2253
EcoRV	GATATC	2 cut(s)	1235, 1960
EcoT14I	CCWWGG	5 cut(s)	51, 956, 1077, 1370, 2639
EcoT22I	ATGCAT	2 cut(s)	1477, 2249
EcoT38I	GRGCYC	6 cut(s)	100, 133, 143, 298, 1077, 2514
EgeI	GGCGCC	1 cut(s)	2078
EheI	GGCGCC	1 cut(s)	2078
ErhI	CCWWGG	5 cut(s)	51, 956, 1077, 1370, 2639
FalI	AAGNNNNNCTT	2 cut(s)	705, 737
FaqI	GGGAC	2 cut(s)	986, 1232
FauNDI	CATATG	3 cut(s)	358, 754, 2748
FblI	GTMKAC	3 cut(s)	651, 1599, 2230
FokI	GGATG	7 cut(s)	236, 917, 1467, 1810, 1814, 2162, 2687
FriOI	GRGCYC	6 cut(s)	100, 133, 143, 298, 1077, 2514
FspBI	CTAG	6 cut(s)	890, 957, 1349, 1682, 2171, 2798
GlaI	GCGC	3 cut(s)	1616, 2078, 2319
GsaI	CCCAGC	1 cut(s)	2770
HaeII	RGCGCY	2 cut(s)	2080, 2321
HaeIII	GGCC	7 cut(s)	559, 1020, 1245, 2501, 2542, 2623, 2779
HapII	CCGG	8 cut(s)	76, 101, 126, 212, 687, 984, 2291, 2624
HhaI	GCGC	3 cut(s)	1617, 2079, 2320
Hin1I	GRCGYC	1 cut(s)	2077
Hin6I	GCGC	3 cut(s)	1615, 2077, 2318
HinP1I	GCGC	3 cut(s)	1615, 2077, 2318
HindIII	AAGCTT	2 cut(s)	2219, 2408
HpaII	CCGG	8 cut(s)	76, 101, 126, 212, 687, 984, 2291, 2624
HphI	GGTGA	7 cut(s)	40, 788, 839, 1286, 2210, 2329, 2657
Hpy166II	GTNNAC	9 cut(s)	280, 652, 1549, 1600, 2066, 2231, 2263, 2374, 2629
Hpy8I	GTNNAC	9 cut(s)	280, 652, 1549, 1600, 2066, 2231, 2263, 2374, 2629
HpyAV	CCTTC	5 cut(s)	194, 490, 1248, 2612, 2644
HpyCH4III	ACNGT	6 cut(s)	529, 1201, 1484, 2419, 2708, 2737
HpyCH4IV	ACGT	4 cut(s)	122, 615, 799, 1978
HpySE526I	ACGT	4 cut(s)	122, 615, 799, 1978
Hsp92I	GRCGYC	1 cut(s)	2077
HspAI	GCGC	3 cut(s)	1615, 2077, 2318
KasI	GGCGCC	1 cut(s)	2076
KflI	GGGWCCC	1 cut(s)	1219
Kpn2I	TCCGGA	2 cut(s)	125, 2290
LguI	GCTCTTC	1 cut(s)	1497
LmnI	GCTCC	9 cut(s)	95, 103, 128, 958, 973, 1072, 1892, 2509, 2517
Lsp1109I	GCAGC	8 cut(s)	315, 406, 595, 1778, 2113, 2116, 2327, 2374
LweI	GCATC	8 cut(s)	599, 940, 1445, 1462, 1916, 2091, 2234, 2399
MaeI	CTAG	6 cut(s)	890, 957, 1349, 1682, 2171, 2798
MaeII	ACGT	4 cut(s)	122, 615, 799, 1978
MaeIII	GTNAC	4 cut(s)	361, 734, 988, 1534
MbiI	CCGCTC	1 cut(s)	375
MfeI	CAATTG	1 cut(s)	1424
MflI	RGATCY	4 cut(s)	1064, 1819, 2278, 2576
MhlI	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1077, 2514, 2604
MlsI	TGGCCA	1 cut(s)	1245
MluNI	TGGCCA	1 cut(s)	1245
Mly113I	GGCGCC	1 cut(s)	2077
MlyI	GAGTC	5 cut(s)	562, 1527, 1936, 2180, 2433
MmeI	TCCRAC	4 cut(s)	739, 1495, 2114, 2438
Mox20I	TGGCCA	1 cut(s)	1245
Mph1103I	ATGCAT	2 cut(s)	1477, 2249
MroI	TCCGGA	2 cut(s)	125, 2290
MroXI	GAANNNNTTC	2 cut(s)	323, 2298
MscI	TGGCCA	1 cut(s)	1245
MseI	TTAA	8 cut(s)	258, 729, 1755, 1826, 1838, 1898, 2046, 2462
MslI	CAYNNNNRTG	2 cut(s)	1622, 2478
Msp20I	TGGCCA	1 cut(s)	1245
MspA1I	CMGCKG	3 cut(s)	1712, 2113, 2126
MspI	CCGG	8 cut(s)	76, 101, 126, 212, 687, 984, 2291, 2624
MspR9I	CCNGG	3 cut(s)	936, 1452, 2255
MunI	CAATTG	1 cut(s)	1424
Mva1269I	GAATGC	2 cut(s)	1051, 2485
MvaI	CCWGG	3 cut(s)	936, 1452, 2255
NarI	GGCGCC	1 cut(s)	2077
NcoI	CCATGG	2 cut(s)	1077, 1370
NdeI	CATATG	3 cut(s)	358, 754, 2748
NheI	GCTAGC	1 cut(s)	1681
NlaIV	GGNNCC	9 cut(s)	933, 1074, 1220, 1221, 1821, 1894, 2078, 2434, 2763
NsiI	ATGCAT	2 cut(s)	1477, 2249
NspI	RCATGY	2 cut(s)	1356, 1627
NspV	TTCGAA	2 cut(s)	335, 1153
PaeI	GCATGC	1 cut(s)	1356
PagI	TCATGA	1 cut(s)	1945
PaqCI	CACCTGC	1 cut(s)	1082
PciSI	GCTCTTC	1 cut(s)	1497
PcsI	WCGNNNNNNNCGW	1 cut(s)	730
PctI	GAATGC	2 cut(s)	1051, 2485
PdmI	GAANNNNTTC	2 cut(s)	323, 2298
PfeI	GAWTC	9 cut(s)	208, 533, 995, 1141, 1150, 1177, 1211, 2041, 2177
PflMI	CCANNNNNTGG	1 cut(s)	1996
PfoI	TCCNGGA	1 cut(s)	1450
Ple19I	CGATCG	1 cut(s)	219
PleI	GAGTC	5 cut(s)	561, 1526, 1936, 2180, 2433
PluTI	GGCGCC	1 cut(s)	2080
PpsI	GAGTC	5 cut(s)	561, 1526, 1936, 2180, 2433
Ppu21I	YACGTR	1 cut(s)	1979
PpuMI	RGGWCCY	1 cut(s)	1219
PsiI	TTATAA	3 cut(s)	470, 788, 1037
Psp124BI	GAGCTC	4 cut(s)	100, 133, 298, 2514
Psp5II	RGGWCCY	1 cut(s)	1219
Psp6I	CCWGG	3 cut(s)	934, 1450, 2253
PspFI	CCCAGC	1 cut(s)	2766
PspGI	CCWGG	3 cut(s)	934, 1450, 2253
PspN4I	GGNNCC	9 cut(s)	933, 1074, 1220, 1221, 1821, 1894, 2078, 2434, 2763
PspPI	GGNCC	4 cut(s)	1219, 2432, 2499, 2621
PspPPI	RGGWCCY	1 cut(s)	1219
PstI	CTGCAG	3 cut(s)	523, 1093, 2733
PstNI	CAGNNNCTG	3 cut(s)	998, 2033, 2129
PsuI	RGATCY	4 cut(s)	1064, 1819, 2278, 2576
PvuI	CGATCG	1 cut(s)	219
PvuII	CAGCTG	2 cut(s)	1712, 2126
RseI	CAYNNNNRTG	2 cut(s)	1622, 2478
SacI	GAGCTC	4 cut(s)	100, 133, 298, 2514
SapI	GCTCTTC	1 cut(s)	1497
SaqAI	TTAA	8 cut(s)	258, 729, 1755, 1826, 1838, 1898, 2046, 2462
Sau96I	GGNCC	4 cut(s)	1219, 2432, 2499, 2621
SbfI	CCTGCAGG	1 cut(s)	1093
SchI	GAGTC	5 cut(s)	562, 1527, 1936, 2180, 2433
ScrFI	CCNGG	3 cut(s)	936, 1452, 2255
SdaI	CCTGCAGG	1 cut(s)	1093
SduI	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1077, 2514, 2604
SfaNI	GCATC	8 cut(s)	599, 940, 1445, 1462, 1916, 2091, 2234, 2399
SfcI	CTRYAG	7 cut(s)	519, 882, 963, 1089, 1830, 2269, 2729
SfoI	GGCGCC	1 cut(s)	2078
SfuI	TTCGAA	2 cut(s)	335, 1153
SinI	GGWCC	2 cut(s)	1219, 2432
SmiI	ATTTAAAT	1 cut(s)	2463
SmiMI	CAYNNNNRTG	2 cut(s)	1622, 2478
SmlI	CTYRAG	4 cut(s)	855, 1172, 1341, 1788
SmoI	CTYRAG	4 cut(s)	855, 1172, 1341, 1788
SphI	GCATGC	1 cut(s)	1356
Sse8387I	CCTGCAGG	1 cut(s)	1093
SspDI	GGCGCC	1 cut(s)	2076
SspMI	CTAG	6 cut(s)	890, 957, 1349, 1682, 2171, 2798
SstI	GAGCTC	4 cut(s)	100, 133, 298, 2514
StyD4I	CCNGG	3 cut(s)	934, 1450, 2253
StyI	CCWWGG	5 cut(s)	51, 956, 1077, 1370, 2639
SwaI	ATTTAAAT	1 cut(s)	2463
TaaI	ACNGT	6 cut(s)	529, 1201, 1484, 2419, 2708, 2737
TaiI	ACGT	4 cut(s)	125, 618, 802, 1981
TaqII	GACCGA	1 cut(s)	1585
TatI	WGTACW	4 cut(s)	1484, 1546, 2371, 2628
TauI	GCSGC	4 cut(s)	42, 1363, 1366, 2018
TfiI	GAWTC	9 cut(s)	208, 533, 995, 1141, 1150, 1177, 1211, 2041, 2177
Tru1I	TTAA	8 cut(s)	258, 729, 1755, 1826, 1838, 1898, 2046, 2462
Tru9I	TTAA	8 cut(s)	258, 729, 1755, 1826, 1838, 1898, 2046, 2462
TscAI	CASTG	6 cut(s)	431, 946, 1487, 1942, 2511, 2742
TseI	GCWGC	8 cut(s)	303, 419, 583, 1766, 2126, 2129, 2315, 2387
TspDTI	ATGAA	9 cut(s)	354, 795, 824, 912, 1203, 1862, 2291, 2706, 2735
TspGWI	ACGGA	4 cut(s)	380, 2196, 2445, 2561
TspRI	CASTG	6 cut(s)	431, 946, 1487, 1942, 2511, 2742
Van91I	CCANNNNNTGG	1 cut(s)	1996
VpaK11BI	GGWCC	2 cut(s)	1219, 2432
XapI	RAATTY	5 cut(s)	319, 513, 1155, 1540, 1922
XceI	RCATGY	2 cut(s)	1356, 1627
XmaJI	CCTAGG	1 cut(s)	956
XmiI	GTMKAC	3 cut(s)	651, 1599, 2230
XmnI	GAANNNNTTC	2 cut(s)	323, 2298
XspI	CTAG	6 cut(s)	890, 957, 1349, 1682, 2171, 2798
Zsp2I	ATGCAT	2 cut(s)	1477, 2249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.