Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014218

Physical Location & Seq

Genomic Coordinates Reverse (-)

18717524 .. 18725934

Gene Structure 8411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1229000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2802 bp

ATGTTGCAGAACAAGTCATTCGTCAAGAGAACCAGAGGCGGCAAAATCACCAAGGTCGTGAGAGAGCACTATCTCCGGCACGATATTTACTGTGGAGCTCCGGTTTGCAAAGTTTGCGATACGTCCGGAGCTCGATTGAGCCCTACCGCTTCCACCATTCTCATCTTCGACACCAATGTCGTCCTTCACCAGATTGATTTGATTGAGAATCCGGCAATAGACGATGTGGTTGTGCTTTCGATTGTGTTGGAAGAGGTTAAAAACAGGAACTTGTCTGTTTACAATAGAGTTAGAGCTCTGTGCAGCAATTCTTTGAGGAAATTTTTCGTCTTTTCGAACGAACACCACAAGGATACTTACGTTACAGACATGAGCGGAGAAAGCAAAAATGATCGAAATGATAGAGCAATTCGGGTAGCTGCTCAATGGTATCAAACTCATCTTGCTGGTTCAGCTAAGGTTTTACTTATAACCAATGATAAAGAGAATAAGAGGAAGGCTATTGAAGAGGGAATTTCTGCAGAGACAGTTGAATCATATGTGAGGTCATTGAGTAGGCCAGAATTGCTCGACCTTCTCGTGCAGCCTGCATCCGAAGATGTAAAGATGGAGGACACTGAAGATTTGAGACCATCAAAAAGGAAAGTCATCTACTCAGAGCATAAGCCCATGTCTGAAATTACTTCTGGCTTGCATCGTGGCATATACCATCAAGGAAAACTTCGAGTTAATCGTTACAACCCCTTTGAAGCATATGTTGGAAGTGAGAGCATTGGTGATGAAATTATAATATATGGACGTACAAATATGAATAGGGCTTTTGATGGTGATATAGTGGCAGTTGAGCTTCTGCCGCAAGATCAATGGCATGAGGAGAAGTCTCTAGCTTTAGCAGATGAAGAGGATGAGGAAGAAGATGTTCATCTGGTTCCAGGCAGTGCTGATGATGCTCCTAGGACTATAGCTGCCCAAGGCTCTGCTGGAGTAACAGAACCTGTCCAAAATCGACCATCTGGCCGTGTTGTTGGTATTATAAAGCGGAATTGGCATTCTTATTGTGGATCTTTGGAGCCCATGGCTAAGCCTGCGGGTGGTGGTGGTATTGCCCATGCTTTATTTGTCTCAAAAGATCGAAGGATTCCAAAGATTCGAATTCAAACTCGACAGCTTGAGAATCTGATGGATAAGAGAATTATTGTGGCAGTTGATTCATGGGACCGTTTGTCTCGGTATCCTTCTGGCCATTATGTACGAACCATAGGAGTAATAGGTGATAAAGATACTGAAACTGAGGTGGTCTTGATTGAGAATGATATAAATACAAGACCATTTTCTTCTCAAGTTCTTGCATGCTTGCCACCGCTACCATGGTTTGTGTCTTCTGAAGATTTGGCAAATCCTATTAGACAGGATTTGCGACAATTGCGTGTCTTTAGTGTTGATCCTCCTGGATGCAAGGATATTGATGATGCATTGCACTGTACAGCTCTGCCAAATGGAAATTATGAAGTTGGAGTCCATATTGCTGATGTAACAAATTTTGTACACTCGGGAACTCCTCTTGATGATGAGGCTTCACAAAGAGGCACATCGGTCTACCTTGTTGAACGGCGCATTGACATGCTTCCAAAACCTCTGACGGAAGATATATGTTCTCTTCGAGCTGATGTGGAAAGGCTAGCTTTTTCTGTTATTTGGGAAATGACAGCTGAAGCTGAGATCATTTCTACAAGATACACCAAAAGTGTGATTAAATCATGTGCAGCATTATCTTACATTGAAGCTCAAGCGAGGATGGATGATAGTCGGTTGATGGATCCATTAACTACAGATTTAAGAAACATGAACTCTTTAGCAAAGATAATGAGGGCAAGGCGTATTGAGAGAGGAGCCTTAACTCTTGCATCTGCTGAAGTCAAATTTGAAATTGACACAGAGACTCATGATCCTCTTGATATCGGTATGTACCAGATTCGTGAAGCAAATCAAATGGTGGAGGAGTTTATGCTTGCCGCTAATGTTTCAGTTGCTGAGAAAATTCTTAAACATTTTCCTCTATGTTCACTATTGAGGCGGCATCCTTCTCCTACCAGAGAGATGCTTGAACCATTGTTGCGTACAGCTGCAGCTATTGGTCTGAACTTGGATGTCTCATCATCAAAGGCACTAGCTGATTCACTTGACTCTGCTGTGGGTAATGATCCGTACTTCAACAAGCTGATTCGGATTTTGGCTACAAGATGCATGACCCAGGCTGTTTACTTCTGTAGTGGGGATCTTAGCCCTCCAGAATATCTTCACTATGGGCTTGCAGCATCTCTATACACTCATTTCACCTCTCCTATTAGGAGATATGCAGATGTCATAACACACAGGTTGCTTGCTGCATCTTTGGGGATATACAAGCTTCCAACTATATTCCAAGATGGACCCAGACTCACAAGCATTGCTGACAATTTAAATTATCGGCATAGGAATGCCCAGATGGCAAGTCGGGCCTCAGTGGAGCTCCATACTGTCATTTTTTTCAAAAAGCGGCCGACAGACACAGAGGCAAGAATAGTCAGAATACGATCCAACGGATTTTTCGTATTTGTGCCCAAGTACGGCATAGAAGGGCCGGTGTACTTGACCCCAAGGGGTGAGAAGGGGGGCGGAGAATGGTATGTAGATGAGCAACAACAGAAGATTAGAAAAATGGACGGTAGTATTTCATACAGCGTTATGCAGACGGTGTTTATTCACATGGAGATTGTGGAACCCCAGCCCAACAGGCCAAAACTCCAACTATCGCTAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000176 GO:0000178 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0004532 GO:0004540 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0019899 GO:0022613 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0051020 GO:0065007 GO:0065009 GO:0070013 GO:0071034 GO:0071043 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1905354

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PIN_4 RNB Rrp44_CSD1 Rrp44_S1

Protein Analysis

934

Amino Acids

104.96

Weight (kDa)

6.33

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIN_4	PF13638	54 - 177	3.4e-21	PIN domain
Rrp44_CSD1	PF17216	219 - 315	9.3e-14	Rrp44-like cold shock domain
OB_HELZ2	PF25049	235 - 292	4.2e-06	3'-5' exoribonuclease HELZ2, OB-fold domain
OB_Dis3	PF17849	371 - 438	6.9e-21	Dis3-like cold-shock domain 2 (CSD2)
RNB	PF00773	469 - 798	5.8e-100	RNB domain
Rrp44_S1	PF17215	850 - 898	1.3e-06	S1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17510 AT2G17510
fragaria_vesca	FvH4_1g10170 FvH4_1g10170
malus_domestica	MD02G1113900.v1.1 MD15G1229000.v1.1
prunus_persica	Prupe.7G185100_v2.0.a1
pyrus_communis	pycom02g08870 pycom15g20420
rosa_chinensis	RchiOBHm_Chr2g0096961
rosa_laevigata	RLG00000016687
rosa_multiflora	Rmu_sc0001651.1_g000008
rosa_roxburghii	Rroxscaffold_2G00145120
rosa_rugosa	Rorug02G0062300
rosa_samantha	Rh2AG110300 Rh2BG112100
rosa_wichuraiana	Rw2G008530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	470, 788, 1034
AasI	GACNNNNNNGTC	1 cut(s)	176
AccBSI	CCGCTC	1 cut(s)	375
AccI	GTMKAC	1 cut(s)	1596
AccIII	TCCGGA	1 cut(s)	125
AclWI	GGATC	8 cut(s)	1069, 1436, 1811, 1824, 1940, 2195, 2283, 2568
AcoI	YGGCCR	3 cut(s)	1015, 1240, 2537
AcsI	RAATTY	6 cut(s)	320, 513, 1152, 1537, 1919, 2037
AcuI	CTGAAG	4 cut(s)	639, 1404, 1731, 1932
AfaI	GTAC	9 cut(s)	802, 1251, 1483, 1545, 1967, 2119, 2207, 2606, 2627
AfiI	CCNNNNNNNGG	2 cut(s)	1091, 2606
AhdI	GACNNNNNGTC	1 cut(s)	1222
AjnI	CCWGG	3 cut(s)	931, 1447, 2250
AjuI	GAANNNNNNNTTGG	4 cut(s)	741, 773, 2570, 2602
Alw21I	GWGCWC	5 cut(s)	69, 100, 133, 298, 2511
Alw26I	GTCTC	7 cut(s)	518, 622, 885, 1126, 1230, 1931, 2155
AlwI	GGATC	8 cut(s)	1069, 1436, 1811, 1824, 1940, 2195, 2283, 2568
AlwNI	CAGNNNCTG	2 cut(s)	995, 2030
Ama87I	CYCGRG	1 cut(s)	1549
Aor13HI	TCCGGA	1 cut(s)	125
AoxI	GGCC	7 cut(s)	557, 1015, 1240, 2496, 2537, 2618, 2774
ApeKI	GCWGC	9 cut(s)	303, 419, 583, 965, 1763, 2123, 2126, 2312, 2384
ApoI	RAATTY	6 cut(s)	320, 513, 1152, 1537, 1919, 2037
Asp700I	GAANNNNTTC	3 cut(s)	323, 918, 2295
AspA2I	CCTAGG	1 cut(s)	953
AspLEI	GCGC	1 cut(s)	1614
AspS9I	GGNCC	4 cut(s)	1216, 2429, 2496, 2618
AsuHPI	GGTGA	7 cut(s)	40, 179, 788, 839, 1283, 2326, 2654
AsuII	TTCGAA	2 cut(s)	335, 1150
AsuNHI	GCTAGC	1 cut(s)	1678
AvaI	CYCGRG	1 cut(s)	1549
AvaII	GGWCC	2 cut(s)	1216, 2429
AvrII	CCTAGG	1 cut(s)	953
BaeGI	GKGCMC	1 cut(s)	2601
BaeI	ACNNNNGTAYC	2 cut(s)	345, 378
BalI	TGGCCA	1 cut(s)	1242
BamHI	GGATCC	1 cut(s)	1816
BanII	GRGCYC	6 cut(s)	100, 133, 143, 298, 1074, 2511
BauI	CACGAG	1 cut(s)	578
BbsI	GAAGAC	1 cut(s)	1371
Bbv12I	GWGCWC	5 cut(s)	69, 100, 133, 298, 2511
BbvI	GCAGC	9 cut(s)	315, 406, 595, 952, 1775, 2110, 2138, 2324, 2371
BceAI	ACGGC	3 cut(s)	1002, 1625, 2623
BciT130I	CCWGG	3 cut(s)	933, 1449, 2252
BciVI	GTATCC	2 cut(s)	346, 1242
BcoDI	GTCTC	7 cut(s)	518, 622, 885, 1126, 1230, 1931, 2155
BfaI	CTAG	5 cut(s)	884, 954, 1679, 2168, 2795
BfmI	CTRYAG	5 cut(s)	519, 960, 1827, 2124, 2266
BfuI	GTATCC	2 cut(s)	346, 1242
BglI	GCCNNNNNGGC	2 cut(s)	2486, 2773
BlnI	CCTAGG	1 cut(s)	953
BlpI	GCTNAGC	1 cut(s)	1080
Bme1390I	CCNGG	3 cut(s)	933, 1449, 2252
Bme18I	GGWCC	2 cut(s)	1216, 2429
BmeRI	GACNNNNNGTC	1 cut(s)	1222
BmeT110I	CYCGRG	1 cut(s)	1549
BmgT120I	GGNCC	4 cut(s)	1216, 2429, 2496, 2618
BmiI	GGNNCC	7 cut(s)	930, 1071, 1217, 1818, 1891, 2431, 2760
BmrFI	CCNGG	3 cut(s)	933, 1449, 2252
BmtI	GCTAGC	1 cut(s)	1682
BpiI	GAAGAC	1 cut(s)	1371
BpmI	CTGGAG	2 cut(s)	1002, 2271
Bpu10I	CCTNAGC	1 cut(s)	456
Bpu1102I	GCTNAGC	1 cut(s)	1080
Bpu14I	TTCGAA	2 cut(s)	335, 1150
BpuEI	CTTGAG	3 cut(s)	1190, 1323, 1770
BsaBI	GATNNNNATC	1 cut(s)	921
BsaI	GGTCTC	1 cut(s)	622
BsaJI	CCNNGG	7 cut(s)	51, 953, 970, 1074, 1367, 2250, 2636
BsaWI	WCCGGW	2 cut(s)	100, 125
BsaXI	ACNNNNNCTCC	2 cut(s)	87, 117
Bsc4I	CCNNNNNNNGG	2 cut(s)	1091, 2606
Bse118I	RCCGGY	1 cut(s)	2620
Bse3DI	GCAATG	2 cut(s)	1472, 2445
Bse8I	GATNNNNATC	1 cut(s)	921
BseAI	TCCGGA	1 cut(s)	125
BseBI	CCWGG	3 cut(s)	933, 1449, 2252
BseDI	CCNNGG	7 cut(s)	51, 953, 970, 1074, 1367, 2250, 2636
BseGI	GGATG	7 cut(s)	590, 910, 1457, 1800, 1804, 2077, 2152
BseJI	GATNNNNATC	1 cut(s)	921
BseLI	CCNNNNNNNGG	2 cut(s)	1091, 2606
BseMI	GCAATG	2 cut(s)	1472, 2445
BseMII	CTCAG	5 cut(s)	669, 1281, 1707, 2022, 2514
BseRI	GAGGAG	4 cut(s)	887, 1548, 1902, 2012
BseSI	GKGCMC	1 cut(s)	2601
BseX3I	CGGCCG	1 cut(s)	2537
BseXI	GCAGC	9 cut(s)	315, 406, 595, 952, 1775, 2110, 2138, 2324, 2371
BseYI	CCCAGC	1 cut(s)	2763
BsgI	GTGCAG	3 cut(s)	322, 602, 1782
Bsh1285I	CGRYCG	1 cut(s)	2540
BshFI	GGCC	7 cut(s)	559, 1017, 1242, 2498, 2539, 2620, 2776
BsiEI	CGRYCG	1 cut(s)	2540
BsiHKAI	GWGCWC	5 cut(s)	69, 100, 133, 298, 2511
BsiHKCI	CYCGRG	1 cut(s)	1549
BsiSI	CCGG	5 cut(s)	76, 101, 126, 212, 2621
BslFI	GGGAC	1 cut(s)	1229
BslI	CCNNNNNNNGG	2 cut(s)	1091, 2606
BsmAI	GTCTC	7 cut(s)	518, 622, 885, 1126, 1230, 1931, 2155
BsmFI	GGGAC	1 cut(s)	1229
BsmI	GAATGC	2 cut(s)	1048, 2482
BsnI	GGCC	7 cut(s)	559, 1017, 1242, 2498, 2539, 2620, 2776
Bso31I	GGTCTC	1 cut(s)	622
BsoBI	CYCGRG	1 cut(s)	1549
Bsp119I	TTCGAA	2 cut(s)	335, 1150
Bsp1286I	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1074, 2511, 2601
Bsp13I	TCCGGA	1 cut(s)	125
Bsp1407I	TGTACA	2 cut(s)	1481, 1543
Bsp1720I	GCTNAGC	1 cut(s)	1080
Bsp19I	CCATGG	2 cut(s)	1074, 1367
BspANI	GGCC	7 cut(s)	559, 1017, 1242, 2498, 2539, 2620, 2776
BspCNI	CTCAG	5 cut(s)	668, 1282, 1708, 2023, 2513
BspEI	TCCGGA	1 cut(s)	125
BspHI	TCATGA	1 cut(s)	1942
BspLI	GGNNCC	7 cut(s)	930, 1071, 1217, 1818, 1891, 2431, 2760
BspMAI	CTGCAG	2 cut(s)	523, 2128
BspOI	GCTAGC	1 cut(s)	1682
BspPI	GGATC	8 cut(s)	1069, 1436, 1811, 1824, 1940, 2195, 2283, 2568
BspT104I	TTCGAA	2 cut(s)	335, 1150
BspTNI	GGTCTC	1 cut(s)	622
BsrBI	CCGCTC	1 cut(s)	375
BsrDI	GCAATG	2 cut(s)	1472, 2445
BsrFI	RCCGGY	1 cut(s)	2620
BsrGI	TGTACA	2 cut(s)	1481, 1543
BssAI	RCCGGY	1 cut(s)	2620
BssECI	CCNNGG	7 cut(s)	51, 953, 970, 1074, 1367, 2250, 2636
BssSI	CACGAG	1 cut(s)	578
BssT1I	CCWWGG	6 cut(s)	51, 953, 970, 1074, 1367, 2636
Bst2BI	CACGAG	1 cut(s)	578
Bst2UI	CCWGG	3 cut(s)	933, 1449, 2252
Bst4CI	ACNGT	7 cut(s)	92, 529, 1220, 1481, 2518, 2705, 2734
Bst6I	CTCTTC	4 cut(s)	246, 501, 894, 1662
BstAPI	GCANNNNNTGC	1 cut(s)	114
BstAUI	TGTACA	2 cut(s)	1481, 1543
BstBI	TTCGAA	2 cut(s)	335, 1150
BstC8I	GCNNGC	9 cut(s)	588, 692, 1086, 1351, 1355, 1680, 2010, 2310, 2382
BstDEI	CTNAG	8 cut(s)	456, 655, 1080, 1290, 1716, 2031, 2279, 2500
BstDSI	CCRYGG	2 cut(s)	1074, 1367
BstF5I	GGATG	7 cut(s)	590, 910, 1457, 1800, 1804, 2077, 2152
BstHHI	GCGC	1 cut(s)	1614
BstMAI	GTCTC	7 cut(s)	518, 622, 885, 1126, 1230, 1931, 2155
BstMCI	CGRYCG	1 cut(s)	2540
BstNI	CCWGG	3 cut(s)	933, 1449, 2252
BstNSI	RCATGY	2 cut(s)	1353, 1624
BstSCI	CCNGG	3 cut(s)	931, 1447, 2250
BstSFI	CTRYAG	5 cut(s)	519, 960, 1827, 2124, 2266
BstSLI	GKGCMC	1 cut(s)	2601
BstV1I	GCAGC	9 cut(s)	315, 406, 595, 952, 1775, 2110, 2138, 2324, 2371
BstV2I	GAAGAC	1 cut(s)	1371
BstX2I	RGATCY	3 cut(s)	1061, 1816, 2275
BstYI	RGATCY	3 cut(s)	1061, 1816, 2275
BstZI	CGGCCG	1 cut(s)	2537
BsuI	GTATCC	2 cut(s)	346, 1242
BsuRI	GGCC	7 cut(s)	559, 1017, 1242, 2498, 2539, 2620, 2776
BtgI	CCRYGG	2 cut(s)	1074, 1367
BtsCI	GGATG	7 cut(s)	590, 910, 1457, 1800, 1804, 2077, 2152
BtsI	GCAGTG	1 cut(s)	943
BtsIMutI	CAGTG	4 cut(s)	615, 943, 1477, 2508
Cac8I	GCNNGC	9 cut(s)	588, 692, 1086, 1351, 1355, 1680, 2010, 2310, 2382
CaiI	CAGNNNCTG	2 cut(s)	995, 2030
CciI	TCATGA	1 cut(s)	1942
CfoI	GCGC	1 cut(s)	1614
Cfr10I	RCCGGY	1 cut(s)	2620
Cfr13I	GGNCC	4 cut(s)	1216, 2429, 2496, 2618
Csp6I	GTAC	9 cut(s)	801, 1250, 1482, 1544, 1966, 2118, 2206, 2605, 2626
CviQI	GTAC	9 cut(s)	801, 1250, 1482, 1544, 1966, 2118, 2206, 2605, 2626
DdeI	CTNAG	8 cut(s)	456, 655, 1080, 1290, 1716, 2031, 2279, 2500
DraI	TTTAAA	1 cut(s)	2460
DrdI	GACNNNNNNGTC	1 cut(s)	176
DriI	GACNNNNNGTC	1 cut(s)	1222
DseDI	GACNNNNNNGTC	1 cut(s)	176
EaeI	YGGCCR	3 cut(s)	1015, 1240, 2537
EagI	CGGCCG	1 cut(s)	2537
Eam1104I	CTCTTC	4 cut(s)	246, 501, 894, 1662
Eam1105I	GACNNNNNGTC	1 cut(s)	1222
EarI	CTCTTC	4 cut(s)	246, 501, 894, 1662
EciI	GGCGGA	1 cut(s)	2670
Ecl136II	GAGCTC	4 cut(s)	98, 131, 296, 2509
EclXI	CGGCCG	1 cut(s)	2537
Eco130I	CCWWGG	6 cut(s)	51, 953, 970, 1074, 1367, 2636
Eco24I	GRGCYC	6 cut(s)	100, 133, 143, 298, 1074, 2511
Eco31I	GGTCTC	1 cut(s)	622
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	2 cut(s)	1216, 2429
Eco52I	CGGCCG	1 cut(s)	2537
Eco53kI	GAGCTC	4 cut(s)	98, 131, 296, 2509
Eco57I	CTGAAG	4 cut(s)	639, 1404, 1731, 1932
Eco88I	CYCGRG	1 cut(s)	1549
EcoICRI	GAGCTC	4 cut(s)	98, 131, 296, 2509
EcoRI	GAATTC	1 cut(s)	1152
EcoRII	CCWGG	3 cut(s)	931, 1447, 2250
EcoRV	GATATC	1 cut(s)	1957
EcoT14I	CCWWGG	6 cut(s)	51, 953, 970, 1074, 1367, 2636
EcoT22I	ATGCAT	2 cut(s)	1474, 2246
EcoT38I	GRGCYC	6 cut(s)	100, 133, 143, 298, 1074, 2511
ErhI	CCWWGG	6 cut(s)	51, 953, 970, 1074, 1367, 2636
FalI	AAGNNNNNCTT	4 cut(s)	705, 737, 1666, 1698
FaqI	GGGAC	1 cut(s)	1229
FauI	CCCGC	1 cut(s)	1081
FauNDI	CATATG	2 cut(s)	538, 754
FblI	GTMKAC	1 cut(s)	1596
FokI	GGATG	7 cut(s)	577, 917, 1464, 1807, 1811, 2064, 2159
FriOI	GRGCYC	6 cut(s)	100, 133, 143, 298, 1074, 2511
FspBI	CTAG	5 cut(s)	884, 954, 1679, 2168, 2795
GlaI	GCGC	1 cut(s)	1613
GsaI	CCCAGC	1 cut(s)	2767
GsuI	CTGGAG	2 cut(s)	1002, 2271
HaeIII	GGCC	7 cut(s)	559, 1017, 1242, 2498, 2539, 2620, 2776
HapII	CCGG	5 cut(s)	76, 101, 126, 212, 2621
HhaI	GCGC	1 cut(s)	1614
Hin6I	GCGC	1 cut(s)	1612
HinP1I	GCGC	1 cut(s)	1612
HindIII	AAGCTT	1 cut(s)	2405
HpaII	CCGG	5 cut(s)	76, 101, 126, 212, 2621
HphI	GGTGA	7 cut(s)	40, 179, 788, 839, 1283, 2326, 2654
Hpy166II	GTNNAC	6 cut(s)	280, 1546, 1597, 2063, 2260, 2626
Hpy188I	TCNGA	9 cut(s)	595, 658, 676, 1179, 1384, 1638, 2139, 2226, 2567
Hpy8I	GTNNAC	6 cut(s)	280, 1546, 1597, 2063, 2260, 2626
HpyAV	CCTTC	8 cut(s)	194, 490, 584, 1128, 1245, 2091, 2609, 2641
HpyCH4III	ACNGT	7 cut(s)	92, 529, 1220, 1481, 2518, 2705, 2734
HpyCH4IV	ACGT	3 cut(s)	122, 360, 799
HpyF3I	CTNAG	8 cut(s)	456, 655, 1080, 1290, 1716, 2031, 2279, 2500
HpySE526I	ACGT	3 cut(s)	122, 360, 799
HspAI	GCGC	1 cut(s)	1612
Kpn2I	TCCGGA	1 cut(s)	125
LmnI	GCTCC	8 cut(s)	95, 103, 128, 955, 1069, 1889, 2506, 2514
Lsp1109I	GCAGC	9 cut(s)	315, 406, 595, 952, 1775, 2110, 2138, 2324, 2371
MaeI	CTAG	5 cut(s)	884, 954, 1679, 2168, 2795
MaeII	ACGT	3 cut(s)	122, 360, 799
MaeIII	GTNAC	4 cut(s)	361, 734, 985, 1531
MbiI	CCGCTC	1 cut(s)	375
MfeI	CAATTG	1 cut(s)	1421
MflI	RGATCY	3 cut(s)	1061, 1816, 2275
MhlI	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1074, 2511, 2601
MlsI	TGGCCA	1 cut(s)	1242
MluNI	TGGCCA	1 cut(s)	1242
MlyI	GAGTC	4 cut(s)	1524, 1933, 2177, 2430
MmeI	TCCRAC	5 cut(s)	228, 739, 1492, 2435, 2601
Mox20I	TGGCCA	1 cut(s)	1242
Mph1103I	ATGCAT	2 cut(s)	1474, 2246
MroI	TCCGGA	1 cut(s)	125
MroXI	GAANNNNTTC	3 cut(s)	323, 918, 2295
MscI	TGGCCA	1 cut(s)	1242
MseI	TTAA	8 cut(s)	258, 729, 1752, 1823, 1835, 1895, 2043, 2459
MslI	CAYNNNNRTG	2 cut(s)	1619, 2475
Msp20I	TGGCCA	1 cut(s)	1242
MspA1I	CMGCKG	2 cut(s)	1709, 2123
MspI	CCGG	5 cut(s)	76, 101, 126, 212, 2621
MspR9I	CCNGG	3 cut(s)	933, 1449, 2252
MunI	CAATTG	1 cut(s)	1421
Mva1269I	GAATGC	2 cut(s)	1048, 2482
MvaI	CCWGG	3 cut(s)	933, 1449, 2252
NcoI	CCATGG	2 cut(s)	1074, 1367
NdeI	CATATG	2 cut(s)	538, 754
NheI	GCTAGC	1 cut(s)	1678
NlaIV	GGNNCC	7 cut(s)	930, 1071, 1217, 1818, 1891, 2431, 2760
NsiI	ATGCAT	2 cut(s)	1474, 2246
NspI	RCATGY	2 cut(s)	1353, 1624
NspV	TTCGAA	2 cut(s)	335, 1150
PaeI	GCATGC	1 cut(s)	1353
PagI	TCATGA	1 cut(s)	1942
PcsI	WCGNNNNNNNCGW	3 cut(s)	576, 730, 2586
PctI	GAATGC	2 cut(s)	1048, 2482
PdmI	GAANNNNTTC	3 cut(s)	323, 918, 2295
PfeI	GAWTC	9 cut(s)	208, 533, 1138, 1147, 1174, 1208, 1972, 2174, 2221
PfoI	TCCNGGA	1 cut(s)	1447
PleI	GAGTC	4 cut(s)	1523, 1933, 2177, 2430
PpsI	GAGTC	4 cut(s)	1523, 1933, 2177, 2430
PsiI	TTATAA	3 cut(s)	470, 788, 1034
Psp124BI	GAGCTC	4 cut(s)	100, 133, 298, 2511
Psp6I	CCWGG	3 cut(s)	931, 1447, 2250
PspFI	CCCAGC	1 cut(s)	2763
PspGI	CCWGG	3 cut(s)	931, 1447, 2250
PspN4I	GGNNCC	7 cut(s)	930, 1071, 1217, 1818, 1891, 2431, 2760
PspPI	GGNCC	4 cut(s)	1216, 2429, 2496, 2618
PstI	CTGCAG	2 cut(s)	523, 2128
PstNI	CAGNNNCTG	2 cut(s)	995, 2030
PsuI	RGATCY	3 cut(s)	1061, 1816, 2275
PvuII	CAGCTG	2 cut(s)	1709, 2123
RsaI	GTAC	9 cut(s)	802, 1251, 1483, 1545, 1967, 2119, 2207, 2606, 2627
RsaNI	GTAC	9 cut(s)	801, 1250, 1482, 1544, 1966, 2118, 2206, 2605, 2626
RseI	CAYNNNNRTG	2 cut(s)	1619, 2475
SacI	GAGCTC	4 cut(s)	100, 133, 298, 2511
SaqAI	TTAA	8 cut(s)	258, 729, 1752, 1823, 1835, 1895, 2043, 2459
Sau96I	GGNCC	4 cut(s)	1216, 2429, 2496, 2618
SchI	GAGTC	4 cut(s)	1524, 1933, 2177, 2430
ScrFI	CCNGG	3 cut(s)	933, 1449, 2252
SduI	GDGCHC	8 cut(s)	69, 100, 133, 143, 298, 1074, 2511, 2601
SfcI	CTRYAG	5 cut(s)	519, 960, 1827, 2124, 2266
SfuI	TTCGAA	2 cut(s)	335, 1150
SinI	GGWCC	2 cut(s)	1216, 2429
SmiI	ATTTAAAT	1 cut(s)	2460
SmiMI	CAYNNNNRTG	2 cut(s)	1619, 2475
SmlI	CTYRAG	3 cut(s)	1169, 1338, 1785
SmoI	CTYRAG	3 cut(s)	1169, 1338, 1785
SphI	GCATGC	1 cut(s)	1353
SspMI	CTAG	5 cut(s)	884, 954, 1679, 2168, 2795
SstI	GAGCTC	4 cut(s)	100, 133, 298, 2511
StyD4I	CCNGG	3 cut(s)	931, 1447, 2250
StyI	CCWWGG	6 cut(s)	51, 953, 970, 1074, 1367, 2636
SwaI	ATTTAAAT	1 cut(s)	2460
TaaI	ACNGT	7 cut(s)	92, 529, 1220, 1481, 2518, 2705, 2734
TaiI	ACGT	3 cut(s)	125, 363, 802
TaqII	GACCGA	1 cut(s)	1582
TatI	WGTACW	3 cut(s)	1481, 1543, 2625
TauI	GCSGC	5 cut(s)	42, 856, 2015, 2077, 2539
TfiI	GAWTC	9 cut(s)	208, 533, 1138, 1147, 1174, 1208, 1972, 2174, 2221
Tru1I	TTAA	8 cut(s)	258, 729, 1752, 1823, 1835, 1895, 2043, 2459
Tru9I	TTAA	8 cut(s)	258, 729, 1752, 1823, 1835, 1895, 2043, 2459
TscAI	CASTG	4 cut(s)	622, 943, 1484, 2508
TseI	GCWGC	9 cut(s)	303, 419, 583, 965, 1763, 2123, 2126, 2312, 2384
TspDTI	ATGAA	8 cut(s)	795, 824, 911, 912, 1200, 1521, 1859, 2703
TspGWI	ACGGA	3 cut(s)	1655, 2193, 2595
TspRI	CASTG	4 cut(s)	622, 943, 1484, 2508
VpaK11BI	GGWCC	2 cut(s)	1216, 2429
XapI	RAATTY	6 cut(s)	320, 513, 1152, 1537, 1919, 2037
XceI	RCATGY	2 cut(s)	1353, 1624
XcmI	CCANNNNNNNNNTGG	2 cut(s)	977, 1365
XmaJI	CCTAGG	1 cut(s)	953
XmiI	GTMKAC	1 cut(s)	1596
XmnI	GAANNNNTTC	3 cut(s)	323, 918, 2295
XspI	CTAG	5 cut(s)	884, 954, 1679, 2168, 2795
Zsp2I	ATGCAT	2 cut(s)	1474, 2246

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.