Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0014218

Physical Location & Seq

Genomic Coordinates Forward (+)

83001116 .. 83008797

Gene Structure 7682 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00145120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGTTGCAGAACCGGTCATACGTCAAGAGAACCAGAGGAGGCAAAATCACCAAGGTCGTCAGAGAGCACTATCTCCGCCATGATATCTACTGCGGAGCTCCGGTATGCAAAGTATGCGACACCTCCCAAGCTCGCCTCAGCGCCACCGCTTCCACCGTCCTCATCCTCGACACCAATGTGGTGCTCAATCAGATTGATTTGATTGAGAATTCGGCGATTGACGACGTGGTTGTGCTTTCGATAGTGCTGGAGGAGGTCAAGAACAGGAACTTGTCCGTTTACAATAGACTTCGAGCTCTATGCAGTAATTCTTTGAGGAAATTCTTTGTTTTCTCTAATGAGCATCACAAGGATACATATGTCACGGACATGAGTGGAGAAAGTAAAAATGATCGAAATGATAGAGCAATTCGGGTGGCTGCTCAATGGTATCAAACTCATTTGGCTGGTTCTGTTCGTATTTTACTTTTAACTAATGACAAAGAGAATAAGAGGAAGGCTCTTGAAGAGGGGATTTCTGCAGAGACAGTGGAGTCGTATGTGAGATCATTGAGTCGACCAGATTTGCTTGATCTGCTTGTGCATCCTGCATCGTCAGAAGATGTAATCATGGAGGAAGTTGAAGATTTAAGACCATCAAAGAAGAAAATTGTTTACTCAGAGCATAAACCCTTGTCAGAGATTACTTCTGGTATCCGGTGCGGTATCTACCATCAAGGAAAACTAAGGGTGAACCGATACAACCCCTTTGAAGCATATGTTGGAAGCCAGAGCCTCAGTGATGAAATAATAATTTATGGACGTGCAAACATGAATAGGGCCTTTGATGGTGATATAGTGGCAGTCCAGCTTCTGCCTCAGGAGCAATGGCATGAGGAGAAATCTCTTGCGATAGCAGATGAAGAGGATGAAGAAGAAGAAGGTGTTCATCTGGTTCCTGGCAGTGCTGATGATGTTCCTAGAACCACAGCTCAACCACAGGATTCTGCTGGAGAAACATGTTCCGTTTCAAGTCGTCCATCTGGCCGTATTGTTGGTATTATAAAGCGGAATTGGCATTCTTATTGTGGATCTTTGGAGCCGATGGCTAAGCCTGCTGGCAGTGGTGGTGTTGCCCATGCCTTATTCGTCTCAAAAGATTGTAGGATTCCTAAGATCCGAATTCAAACTCGACAGCTAGAAAATCTTCTGGACAAGAGAATTATTGTGGCGGTTGATTCTTGGGACTGTTTGTCTCGTTATCCTTCTGGCCATTATGTACGAACCATAGGACAAATCGGTGATAAAGATACTGAAACTGAGGTGGTCTTGATTGAGAATGATATAAATACAAGACCATTTTCTACTCAAGTTCTAGCATGCTTGCCACCATTGCCATGGTCTGTGTCCTCTGCAGATTTGGCAAATCCTGTTAGGGAGGATTTGCGACAATTACGTGTCTTTAGTGTGGACCCTCCTGGATGCAAGGATATCGATGATGCACTTCATTGTACAGCTCTTCCAAATGGAAATTACGAAGTTGGTGTCCATATTGCTGATGTTACAAATTTTGTACTTCCTGGTACCCCTCTTGATGATGAGGCTTCACAAAGGGGCACATCGGTCTACCTTGTCGATCGACGCATTGACATGCTTCCGAAACCTCTGACAGAAGATATATGTTCACTCCGAGCTGATGTGGAAAGGCTAGCCTTTTCTGTTATTTGGGAAATGACATCAGAAGCTGAGATTATATCTACAAGATACACAAAAAGTGTCATTAAATCTTCTGCAGCATTGTCTTATGTAGAAGCTCAAGCAAGGATGGATGATAGCCGGTTGACGGATCCAGTAACTACAGATTTGAGAAACATGAACGCATTAGCAAAGATAATGAGGTCAAGACGTATTGAGAGAGGAGCCTTAACTCTTGCATCCGCTGAAGTCAGATTTCAAATTGACACTGAGACTCATGATCCCCTTGATATTGGTATGTACCAGATACGCGAAGCAAACCAAATGGTTGAGGAGTTTATGCTTGCTGCTAATGTTTCAGTTGCTCAGAAGATTCTTAAACATTATCCTTTGTGCTCACTATTGAGGCGCCATCCTACTCCAACAAGAGAGATGCTTGAACCTTTGTTACGGACAGCTGCTGCTGTTGGTCTCAACTTGGATGTTTCATCATCAAAAGCACTGGCTGATTCACTCGACCATGCTGTGGGTGATGATCCGTACTTTAATAAGCTAATCCGTATATTGGCCACTAGATGCATGACACAGGCCGTTTATTTCTGTAGTGGGGAGCTCAGCCCTCCAGAATATCTTCATTATGGGCTTGCAGCACCTCTGTATACCCATTTTACCTCTCCTATAAGGAGATATGCAGATGTCATAGTGCATCGGTTGCTTGCTGCATCTCTGGGTATACACAGGCTTCCAACTGTATTCCAAGATGGACCCCAACTCAATAGTATTGCTGAAAATTTAAACTATCGACATAGGAACGCTCAGATGGCAAGCAGGGCCTCAGTGGAACTCCATACTCTGATTTTTTTCAGAACACGGTTTGGCATAGAAGGGCCTGTGTACCTGACCCCAAGAGGTGAGAAGGGAAGTGGAGAATGGTTTGTAGATGAACAACAGCAGAAGATTAGAAAAATGGATGGCAGCATTTCATACAGCGTTCTGCAGACAGTGAGAATCCATTTGGAGATTGTCGAGCCCCAGCCCAATAGACCGAAACTCGAGCTTAAGCTGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000176 GO:0000178 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0004532 GO:0004540 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0019899 GO:0022613 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0051020 GO:0065007 GO:0065009 GO:0070013 GO:0071034 GO:0071043 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1905354

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PIN_4 RNB Rrp44_CSD1 Rrp44_S1

Protein Analysis

914

Amino Acids

102.63

Weight (kDa)

6.1

Isoelectric Point (pI)

41.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIN_4	PF13638	54 - 177	1.9e-20	PIN domain
Rrp44_CSD1	PF17216	220 - 318	4.3e-13	Rrp44-like cold shock domain
OB_HELZ2	PF25049	236 - 293	2.4e-06	3'-5' exoribonuclease HELZ2, OB-fold domain
OB_Dis3	PF17849	374 - 441	5.3e-21	Dis3-like cold-shock domain 2 (CSD2)
RNB	PF00773	472 - 801	7.8e-101	RNB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17510 AT2G17510
fragaria_vesca	FvH4_1g10170 FvH4_1g10170
malus_domestica	MD02G1113900.v1.1 MD15G1229000.v1.1
prunus_persica	Prupe.7G185100_v2.0.a1
pyrus_communis	pycom02g08870 pycom15g20420
rosa_chinensis	RchiOBHm_Chr2g0096961
rosa_laevigata	RLG00000016687
rosa_multiflora	Rmu_sc0001651.1_g000008
rosa_roxburghii	Rroxscaffold_2G00145120
rosa_rugosa	Rorug02G0062300
rosa_samantha	Rh2AG110300 Rh2BG112100
rosa_wichuraiana	Rw2G008530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1043
AasI	GACNNNNNNGTC	1 cut(s)	532
Acc65I	GGTACC	1 cut(s)	1562
AccB1I	GGYRCC	2 cut(s)	1562, 2082
AccB7I	CCANNNNNTGG	1 cut(s)	2200
AccI	GTMKAC	4 cut(s)	556, 1605, 2333, 2407
AccII	CGCG	1 cut(s)	1986
AciI	CCGC	7 cut(s)	76, 93, 147, 702, 1048, 1211, 1917
AclWI	GGATC	6 cut(s)	1078, 1150, 1820, 1833, 1949, 2204
AcoI	YGGCCR	3 cut(s)	1024, 1249, 2241
AcsI	RAATTY	5 cut(s)	208, 320, 1161, 1546, 2464
AcuI	CTGAAG	1 cut(s)	1941
AcyI	GRCGYC	1 cut(s)	2083
AfaI	GTAC	7 cut(s)	1260, 1492, 1554, 1564, 1976, 2216, 2570
AfiI	CCNNNNNNNGG	3 cut(s)	1822, 2200, 2239
AflII	CTTAAG	1 cut(s)	2732
AflIII	ACRYGT	2 cut(s)	998, 1435
AgeI	ACCGGT	1 cut(s)	12
AgsI	TTSAA	7 cut(s)	506, 623, 752, 1011, 1166, 1934, 2114
AhdI	GACNNNNNGTC	1 cut(s)	1231
AjiI	CACGTC	2 cut(s)	226, 803
AjnI	CCWGG	3 cut(s)	937, 1456, 1558
AjuI	GAANNNNNNNTTGG	2 cut(s)	744, 776
AleI	CACNNNNGTG	1 cut(s)	176
Alw21I	GWGCWC	6 cut(s)	69, 100, 186, 298, 2072, 2289
Alw26I	GTCTC	5 cut(s)	518, 1135, 1239, 1940, 2150
AlwI	GGATC	6 cut(s)	1078, 1150, 1820, 1833, 1949, 2204
AlwNI	CAGNNNCTG	3 cut(s)	853, 1724, 2135
Ama87I	CYCGRG	1 cut(s)	2726
AoxI	GGCC	7 cut(s)	819, 1024, 1249, 2241, 2262, 2505, 2561
ApeKI	GCWGC	8 cut(s)	419, 1772, 2021, 2132, 2135, 2321, 2393, 2649
ApoI	RAATTY	5 cut(s)	208, 320, 1161, 1546, 2464
ArsI	GACNNNNNNTTYG	2 cut(s)	1509, 1541
AsiGI	ACCGGT	1 cut(s)	12
Asp700I	GAANNNNTTC	3 cut(s)	924, 1185, 2304
Asp718I	GGTACC	1 cut(s)	1562
AspLEI	GCGC	2 cut(s)	143, 2085
AspS9I	GGNCC	5 cut(s)	819, 1450, 2438, 2505, 2561
AsuHPI	GGTGA	6 cut(s)	40, 742, 842, 1292, 2216, 2597
AsuNHI	GCTAGC	1 cut(s)	1687
AvaI	CYCGRG	1 cut(s)	2726
AvaII	GGWCC	2 cut(s)	1450, 2438
AxyI	CCTNAGG	1 cut(s)	858
BaeGI	GKGCMC	1 cut(s)	1598
BaeI	ACNNNNGTAYC	2 cut(s)	345, 378
BalI	TGGCCA	2 cut(s)	1251, 2243
BamHI	GGATCC	1 cut(s)	1825
BanI	GGYRCC	2 cut(s)	1562, 2082
BanII	GRGCYC	4 cut(s)	100, 298, 2289, 2706
Bbv12I	GWGCWC	6 cut(s)	69, 100, 186, 298, 2072, 2289
BbvCI	CCTCAGC	1 cut(s)	137
BbvI	GCAGC	8 cut(s)	406, 1784, 2008, 2119, 2122, 2333, 2380, 2661
BceAI	ACGGC	2 cut(s)	1011, 2249
BcgI	CGANNNNNNTGC	4 cut(s)	282, 316, 1453, 1487
BciT130I	CCWGG	3 cut(s)	939, 1458, 1560
BciVI	GTATCC	2 cut(s)	346, 704
BcoDI	GTCTC	5 cut(s)	518, 1135, 1239, 1940, 2150
BfaI	CTAG	5 cut(s)	960, 1178, 1355, 1688, 2247
BfmI	CTRYAG	6 cut(s)	519, 1392, 1770, 1836, 2275, 2669
BfoI	RGCGCY	2 cut(s)	144, 2086
BfrI	CTTAAG	1 cut(s)	2732
BfuI	GTATCC	2 cut(s)	346, 704
BisI	GCNGC	8 cut(s)	420, 1773, 2022, 2133, 2136, 2322, 2394, 2650
BlpI	GCTNAGC	2 cut(s)	1089, 2288
BlsI	GCNGC	8 cut(s)	421, 1774, 2023, 2134, 2137, 2323, 2395, 2651
Bme1390I	CCNGG	3 cut(s)	939, 1458, 1560
Bme18I	GGWCC	2 cut(s)	1450, 2438
BmeRI	GACNNNNNGTC	1 cut(s)	1231
BmeT110I	CYCGRG	1 cut(s)	2726
BmgBI	CACGTC	2 cut(s)	226, 803
BmgT120I	GGNCC	5 cut(s)	819, 1450, 2438, 2505, 2561
BmiI	GGNNCC	8 cut(s)	936, 1080, 1452, 1564, 1827, 1900, 2084, 2440
BmrFI	CCNGG	3 cut(s)	939, 1458, 1560
BmtI	GCTAGC	1 cut(s)	1691
BplI	GAGNNNNNCTC	2 cut(s)	484, 516
BpmI	CTGGAG	3 cut(s)	269, 1011, 2280
Bpu10I	CCTNAGC	1 cut(s)	137
Bpu1102I	GCTNAGC	2 cut(s)	1089, 2288
BpuEI	CTTGAG	2 cut(s)	1332, 1779
Bsa29I	ATCGAT	1 cut(s)	1473
BsaAI	YACGTR	1 cut(s)	1436
BsaHI	GRCGYC	1 cut(s)	2083
BsaI	GGTCTC	1 cut(s)	2150
BsaJI	CCNNGG	2 cut(s)	51, 1376
BsaWI	WCCGGW	3 cut(s)	12, 100, 696
BsaXI	ACNNNNNCTCC	2 cut(s)	87, 117
Bsc4I	CCNNNNNNNGG	3 cut(s)	1822, 2200, 2239
Bse118I	RCCGGY	2 cut(s)	12, 1815
Bse1I	ACTGG	2 cut(s)	1829, 2181
Bse21I	CCTNAGG	1 cut(s)	858
Bse3DI	GCAATG	2 cut(s)	872, 1370
BseBI	CCWGG	3 cut(s)	939, 1458, 1560
BseCI	ATCGAT	1 cut(s)	1473
BseDI	CCNNGG	2 cut(s)	51, 1376
BseLI	CCNNNNNNNGG	3 cut(s)	1822, 2200, 2239
BseMI	GCAATG	2 cut(s)	872, 1370
BseNI	ACTGG	2 cut(s)	1829, 2181
BseRI	GAGGAG	5 cut(s)	51, 266, 890, 1911, 2021
BseSI	GKGCMC	1 cut(s)	1598
BseXI	GCAGC	8 cut(s)	406, 1784, 2008, 2119, 2122, 2333, 2380, 2661
BseYI	CCCAGC	1 cut(s)	2706
Bsh1236I	CGCG	1 cut(s)	1986
Bsh1285I	CGRYCG	1 cut(s)	1618
BshFI	GGCC	7 cut(s)	821, 1026, 1251, 2243, 2264, 2507, 2563
BshNI	GGYRCC	2 cut(s)	1562, 2082
BshTI	ACCGGT	1 cut(s)	12
BshVI	ATCGAT	1 cut(s)	1473
BsiEI	CGRYCG	1 cut(s)	1618
BsiHKAI	GWGCWC	6 cut(s)	69, 100, 186, 298, 2072, 2289
BsiHKCI	CYCGRG	1 cut(s)	2726
BsiSI	CCGG	4 cut(s)	13, 101, 697, 1816
BslFI	GGGAC	1 cut(s)	1238
BslI	CCNNNNNNNGG	3 cut(s)	1822, 2200, 2239
BsmAI	GTCTC	5 cut(s)	518, 1135, 1239, 1940, 2150
BsmBI	CGTCTC	1 cut(s)	1135
BsmFI	GGGAC	1 cut(s)	1238
BsmI	GAATGC	1 cut(s)	1057
BsnI	GGCC	7 cut(s)	821, 1026, 1251, 2243, 2264, 2507, 2563
Bso31I	GGTCTC	1 cut(s)	2150
BsoBI	CYCGRG	1 cut(s)	2726
Bsp1286I	GDGCHC	8 cut(s)	69, 100, 186, 298, 1598, 2072, 2289, 2706
Bsp1407I	TGTACA	1 cut(s)	1490
Bsp143I	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
Bsp1720I	GCTNAGC	2 cut(s)	1089, 2288
Bsp19I	CCATGG	1 cut(s)	1376
BspACI	CCGC	7 cut(s)	76, 93, 147, 702, 1048, 1211, 1917
BspANI	GGCC	7 cut(s)	821, 1026, 1251, 2243, 2264, 2507, 2563
BspDI	ATCGAT	1 cut(s)	1473
BspFNI	CGCG	1 cut(s)	1986
BspHI	TCATGA	1 cut(s)	1951
BspLI	GGNNCC	8 cut(s)	936, 1080, 1452, 1564, 1827, 1900, 2084, 2440
BspMAI	CTGCAG	4 cut(s)	523, 1396, 1774, 2673
BspOI	GCTAGC	1 cut(s)	1691
BspPI	GGATC	6 cut(s)	1078, 1150, 1820, 1833, 1949, 2204
BspQI	GCTCTTC	1 cut(s)	1503
BspT107I	GGYRCC	2 cut(s)	1562, 2082
BspTI	CTTAAG	1 cut(s)	2732
BspTNI	GGTCTC	1 cut(s)	2150
BsrDI	GCAATG	2 cut(s)	872, 1370
BsrFI	RCCGGY	2 cut(s)	12, 1815
BsrGI	TGTACA	1 cut(s)	1490
BsrI	ACTGG	2 cut(s)	1829, 2181
BssAI	RCCGGY	2 cut(s)	12, 1815
BssECI	CCNNGG	2 cut(s)	51, 1376
BssMI	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
BssNAI	GTATAC	2 cut(s)	2334, 2408
BssNI	GRCGYC	1 cut(s)	2083
BssT1I	CCWWGG	2 cut(s)	51, 1376
Bst1107I	GTATAC	2 cut(s)	2334, 2408
Bst2UI	CCWGG	3 cut(s)	939, 1458, 1560
Bst4CI	ACNGT	6 cut(s)	157, 529, 1229, 2425, 2547, 2677
Bst6I	CTCTTC	3 cut(s)	501, 897, 1503
BstACI	GRCGYC	1 cut(s)	2083
BstAFI	CTTAAG	1 cut(s)	2732
BstAPI	GCANNNNNTGC	2 cut(s)	114, 2386
BstAUI	TGTACA	1 cut(s)	1490
BstBAI	YACGTR	1 cut(s)	1436
BstDSI	CCRYGG	1 cut(s)	1376
BstFNI	CGCG	1 cut(s)	1986
BstH2I	RGCGCY	2 cut(s)	144, 2086
BstHHI	GCGC	2 cut(s)	143, 2085
BstKTI	GATC	9 cut(s)	394, 548, 574, 1073, 1158, 1618, 1828, 1957, 2212
BstMAI	GTCTC	5 cut(s)	518, 1135, 1239, 1940, 2150
BstMBI	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
BstMCI	CGRYCG	1 cut(s)	1618
BstNI	CCWGG	3 cut(s)	939, 1458, 1560
BstNSI	RCATGY	3 cut(s)	1002, 1362, 1633
BstSCI	CCNGG	3 cut(s)	937, 1456, 1558
BstSFI	CTRYAG	6 cut(s)	519, 1392, 1770, 1836, 2275, 2669
BstSLI	GKGCMC	1 cut(s)	1598
BstUI	CGCG	1 cut(s)	1986
BstV1I	GCAGC	8 cut(s)	406, 1784, 2008, 2119, 2122, 2333, 2380, 2661
BstX2I	RGATCY	3 cut(s)	1070, 1155, 1825
BstXI	CCANNNNNNTGG	1 cut(s)	1377
BstYI	RGATCY	3 cut(s)	1070, 1155, 1825
BstZ17I	GTATAC	2 cut(s)	2334, 2408
Bsu15I	ATCGAT	1 cut(s)	1473
Bsu36I	CCTNAGG	1 cut(s)	858
BsuI	GTATCC	2 cut(s)	346, 704
BsuRI	GGCC	7 cut(s)	821, 1026, 1251, 2243, 2264, 2507, 2563
BsuTUI	ATCGAT	1 cut(s)	1473
BtgI	CCRYGG	1 cut(s)	1376
BtrI	CACGTC	2 cut(s)	226, 803
BtsI	GCAGTG	2 cut(s)	949, 1108
BtsIMutI	CAGTG	8 cut(s)	534, 784, 949, 1108, 1941, 2174, 2517, 2682
CaiI	CAGNNNCTG	3 cut(s)	853, 1724, 2135
CciI	TCATGA	1 cut(s)	1951
CfoI	GCGC	2 cut(s)	143, 2085
Cfr10I	RCCGGY	2 cut(s)	12, 1815
Cfr13I	GGNCC	5 cut(s)	819, 1450, 2438, 2505, 2561
ClaI	ATCGAT	1 cut(s)	1473
CseI	GACGC	1 cut(s)	1629
Csp6I	GTAC	7 cut(s)	1259, 1491, 1553, 1563, 1975, 2215, 2569
CspAI	ACCGGT	1 cut(s)	12
CspCI	CAANNNNNGTGG	2 cut(s)	396, 431
CviQI	GTAC	7 cut(s)	1259, 1491, 1553, 1563, 1975, 2215, 2569
DinI	GGCGCC	1 cut(s)	2084
DpnI	GATC	9 cut(s)	393, 547, 573, 1072, 1157, 1617, 1827, 1956, 2211
DpnII	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
DraI	TTTAAA	1 cut(s)	2469
DrdI	GACNNNNNNGTC	1 cut(s)	532
DriI	GACNNNNNGTC	1 cut(s)	1231
DseDI	GACNNNNNNGTC	1 cut(s)	532
EaeI	YGGCCR	3 cut(s)	1024, 1249, 2241
Eam1104I	CTCTTC	3 cut(s)	501, 897, 1503
Eam1105I	GACNNNNNGTC	1 cut(s)	1231
EarI	CTCTTC	3 cut(s)	501, 897, 1503
EciI	GGCGGA	1 cut(s)	65
Ecl136II	GAGCTC	3 cut(s)	98, 296, 2287
Eco130I	CCWWGG	2 cut(s)	51, 1376
Eco24I	GRGCYC	4 cut(s)	100, 298, 2289, 2706
Eco31I	GGTCTC	1 cut(s)	2150
Eco32I	GATATC	2 cut(s)	85, 1471
Eco47I	GGWCC	2 cut(s)	1450, 2438
Eco53kI	GAGCTC	3 cut(s)	98, 296, 2287
Eco57I	CTGAAG	1 cut(s)	1941
Eco81I	CCTNAGG	1 cut(s)	858
Eco88I	CYCGRG	1 cut(s)	2726
EcoICRI	GAGCTC	3 cut(s)	98, 296, 2287
EcoO109I	RGGNCCY	3 cut(s)	819, 2505, 2561
EcoRI	GAATTC	2 cut(s)	208, 1161
EcoRII	CCWGG	3 cut(s)	937, 1456, 1558
EcoRV	GATATC	2 cut(s)	85, 1471
EcoT14I	CCWWGG	2 cut(s)	51, 1376
EcoT22I	ATGCAT	1 cut(s)	2255
EcoT38I	GRGCYC	4 cut(s)	100, 298, 2289, 2706
EgeI	GGCGCC	1 cut(s)	2084
EheI	GGCGCC	1 cut(s)	2084
ErhI	CCWWGG	2 cut(s)	51, 1376
Esp3I	CGTCTC	1 cut(s)	1135
FaqI	GGGAC	1 cut(s)	1238
FauNDI	CATATG	2 cut(s)	358, 757
FblI	GTMKAC	4 cut(s)	556, 1605, 2333, 2407
Fnu4HI	GCNGC	8 cut(s)	420, 1773, 2022, 2133, 2136, 2322, 2394, 2650
FriOI	GRGCYC	4 cut(s)	100, 298, 2289, 2706
Fsp4HI	GCNGC	8 cut(s)	420, 1773, 2022, 2133, 2136, 2322, 2394, 2650
FspBI	CTAG	5 cut(s)	960, 1178, 1355, 1688, 2247
GlaI	GCGC	2 cut(s)	142, 2084
GluI	GCNGC	8 cut(s)	420, 1773, 2022, 2133, 2136, 2322, 2394, 2650
GsaI	CCCAGC	1 cut(s)	2710
GsuI	CTGGAG	3 cut(s)	269, 1011, 2280
HaeII	RGCGCY	2 cut(s)	144, 2086
HaeIII	GGCC	7 cut(s)	821, 1026, 1251, 2243, 2264, 2507, 2563
HapII	CCGG	4 cut(s)	13, 101, 697, 1816
HgaI	GACGC	1 cut(s)	1629
HhaI	GCGC	2 cut(s)	143, 2085
Hin1I	GRCGYC	1 cut(s)	2083
Hin6I	GCGC	2 cut(s)	141, 2083
HinP1I	GCGC	2 cut(s)	141, 2083
HincII	GTYRAC	2 cut(s)	557, 1821
HindII	GTYRAC	2 cut(s)	557, 1821
HinfI	GANTC	9 cut(s)	533, 553, 983, 1147, 1217, 1948, 2047, 2183, 2682
HpaII	CCGG	4 cut(s)	13, 101, 697, 1816
HphI	GGTGA	6 cut(s)	40, 742, 842, 1292, 2216, 2597
Hpy99I	CGWCG	2 cut(s)	227, 1623
HpyAV	CCTTC	5 cut(s)	490, 914, 1254, 2552, 2584
HpyCH4III	ACNGT	6 cut(s)	157, 529, 1229, 2425, 2547, 2677
HpyCH4IV	ACGT	5 cut(s)	21, 225, 802, 1435, 1885
HpySE526I	ACGT	5 cut(s)	21, 225, 802, 1435, 1885
Hsp92I	GRCGYC	1 cut(s)	2083
HspAI	GCGC	2 cut(s)	141, 2083
KasI	GGCGCC	1 cut(s)	2082
KpnI	GGTACC	1 cut(s)	1566
Kzo9I	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
LguI	GCTCTTC	1 cut(s)	1503
LmnI	GCTCC	6 cut(s)	95, 103, 862, 1078, 1898, 2284
Lsp1109I	GCAGC	8 cut(s)	406, 1784, 2008, 2119, 2122, 2333, 2380, 2661
MaeI	CTAG	5 cut(s)	960, 1178, 1355, 1688, 2247
MaeII	ACGT	5 cut(s)	21, 225, 802, 1435, 1885
MaeIII	GTNAC	4 cut(s)	361, 1540, 1831, 2121
MalI	GATC	9 cut(s)	393, 547, 573, 1072, 1157, 1617, 1827, 1956, 2211
MboI	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
MflI	RGATCY	3 cut(s)	1070, 1155, 1825
MhlI	GDGCHC	8 cut(s)	69, 100, 186, 298, 1598, 2072, 2289, 2706
MlsI	TGGCCA	2 cut(s)	1251, 2243
MluNI	TGGCCA	2 cut(s)	1251, 2243
Mly113I	GGCGCC	1 cut(s)	2083
MlyI	GAGTC	3 cut(s)	542, 562, 1942
MmeI	TCCRAC	3 cut(s)	742, 2120, 2444
Mox20I	TGGCCA	2 cut(s)	1251, 2243
Mph1103I	ATGCAT	1 cut(s)	2255
MroXI	GAANNNNTTC	3 cut(s)	924, 1185, 2304
MscI	TGGCCA	2 cut(s)	1251, 2243
MseI	TTAA	8 cut(s)	470, 629, 1761, 1904, 2052, 2220, 2468, 2733
MslI	CAYNNNNRTG	4 cut(s)	176, 368, 1375, 1628
Msp20I	TGGCCA	2 cut(s)	1251, 2243
MspA1I	CMGCKG	2 cut(s)	1919, 2132
MspCI	CTTAAG	1 cut(s)	2732
MspI	CCGG	4 cut(s)	13, 101, 697, 1816
MspR9I	CCNGG	3 cut(s)	939, 1458, 1560
Mva1269I	GAATGC	1 cut(s)	1057
MvaI	CCWGG	3 cut(s)	939, 1458, 1560
MvnI	CGCG	1 cut(s)	1986
NarI	GGCGCC	1 cut(s)	2083
NcoI	CCATGG	1 cut(s)	1376
NdeI	CATATG	2 cut(s)	358, 757
NdeII	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
NheI	GCTAGC	1 cut(s)	1687
NlaIV	GGNNCC	8 cut(s)	936, 1080, 1452, 1564, 1827, 1900, 2084, 2440
NmuCI	GTSAC	1 cut(s)	361
NsiI	ATGCAT	1 cut(s)	2255
NspI	RCATGY	3 cut(s)	1002, 1362, 1633
OliI	CACNNNNGTG	1 cut(s)	176
PaeI	GCATGC	1 cut(s)	1362
PaeR7I	CTCGAG	1 cut(s)	2726
PagI	TCATGA	1 cut(s)	1951
PciI	ACATGT	1 cut(s)	998
PciSI	GCTCTTC	1 cut(s)	1503
PctI	GAATGC	1 cut(s)	1057
PdmI	GAANNNNTTC	3 cut(s)	924, 1185, 2304
PfeI	GAWTC	6 cut(s)	983, 1147, 1217, 2047, 2183, 2682
PflMI	CCANNNNNTGG	1 cut(s)	2200
PfoI	TCCNGGA	1 cut(s)	1456
PinAI	ACCGGT	1 cut(s)	12
PkrI	GCNGC	8 cut(s)	421, 1774, 2023, 2134, 2137, 2323, 2395, 2651
Ple19I	CGATCG	1 cut(s)	1618
PleI	GAGTC	3 cut(s)	541, 561, 1942
PluTI	GGCGCC	1 cut(s)	2086
PpsI	GAGTC	3 cut(s)	541, 561, 1942
Ppu21I	YACGTR	1 cut(s)	1436
PscI	ACATGT	1 cut(s)	998
PsiI	TTATAA	1 cut(s)	1043
Psp124BI	GAGCTC	3 cut(s)	100, 298, 2289
Psp6I	CCWGG	3 cut(s)	937, 1456, 1558
PspFI	CCCAGC	1 cut(s)	2706
PspGI	CCWGG	3 cut(s)	937, 1456, 1558
PspN4I	GGNNCC	8 cut(s)	936, 1080, 1452, 1564, 1827, 1900, 2084, 2440
PspPI	GGNCC	5 cut(s)	819, 1450, 2438, 2505, 2561
PspXI	VCTCGAGB	1 cut(s)	2726
PsrI	GAACNNNNNNTAC	3 cut(s)	34, 2106, 2138
PstI	CTGCAG	4 cut(s)	523, 1396, 1774, 2673
PstNI	CAGNNNCTG	3 cut(s)	853, 1724, 2135
PsuI	RGATCY	3 cut(s)	1070, 1155, 1825
PvuI	CGATCG	1 cut(s)	1618
PvuII	CAGCTG	1 cut(s)	2132
RsaI	GTAC	7 cut(s)	1260, 1492, 1554, 1564, 1976, 2216, 2570
RsaNI	GTAC	7 cut(s)	1259, 1491, 1553, 1563, 1975, 2215, 2569
RseI	CAYNNNNRTG	4 cut(s)	176, 368, 1375, 1628
SacI	GAGCTC	3 cut(s)	100, 298, 2289
SalI	GTCGAC	1 cut(s)	555
SapI	GCTCTTC	1 cut(s)	1503
SaqAI	TTAA	8 cut(s)	470, 629, 1761, 1904, 2052, 2220, 2468, 2733
SatI	GCNGC	8 cut(s)	420, 1773, 2022, 2133, 2136, 2322, 2394, 2650
Sau3AI	GATC	9 cut(s)	391, 545, 571, 1070, 1155, 1615, 1825, 1954, 2209
Sau96I	GGNCC	5 cut(s)	819, 1450, 2438, 2505, 2561
SchI	GAGTC	3 cut(s)	542, 562, 1942
ScrFI	CCNGG	3 cut(s)	939, 1458, 1560
SduI	GDGCHC	8 cut(s)	69, 100, 186, 298, 1598, 2072, 2289, 2706
SfcI	CTRYAG	6 cut(s)	519, 1392, 1770, 1836, 2275, 2669
SfoI	GGCGCC	1 cut(s)	2084
Sfr274I	CTCGAG	1 cut(s)	2726
SinI	GGWCC	2 cut(s)	1450, 2438
SlaI	CTCGAG	1 cut(s)	2726
SmiMI	CAYNNNNRTG	4 cut(s)	176, 368, 1375, 1628
SmlI	CTYRAG	4 cut(s)	1347, 1794, 2726, 2732
SmoI	CTYRAG	4 cut(s)	1347, 1794, 2726, 2732
SphI	GCATGC	1 cut(s)	1362
SsiI	CCGC	7 cut(s)	76, 93, 147, 702, 1048, 1211, 1917
SspDI	GGCGCC	1 cut(s)	2082
SspMI	CTAG	5 cut(s)	960, 1178, 1355, 1688, 2247
SstI	GAGCTC	3 cut(s)	100, 298, 2289
StyD4I	CCNGG	3 cut(s)	937, 1456, 1558
StyI	CCWWGG	2 cut(s)	51, 1376
TaaI	ACNGT	6 cut(s)	157, 529, 1229, 2425, 2547, 2677
TaiI	ACGT	5 cut(s)	24, 228, 805, 1438, 1888
TaqII	GACCGA	2 cut(s)	1591, 2734
TatI	WGTACW	2 cut(s)	1490, 1552
TfiI	GAWTC	6 cut(s)	983, 1147, 1217, 2047, 2183, 2682
Tru1I	TTAA	8 cut(s)	470, 629, 1761, 1904, 2052, 2220, 2468, 2733
Tru9I	TTAA	8 cut(s)	470, 629, 1761, 1904, 2052, 2220, 2468, 2733
TscAI	CASTG	8 cut(s)	534, 784, 949, 1108, 1948, 2181, 2517, 2682
TseFI	GTSAC	1 cut(s)	361
TseI	GCWGC	8 cut(s)	419, 1772, 2021, 2132, 2135, 2321, 2393, 2649
Tsp45I	GTSAC	1 cut(s)	361
TspGWI	ACGGA	7 cut(s)	265, 380, 994, 1838, 2140, 2202, 2222
TspRI	CASTG	8 cut(s)	534, 784, 949, 1108, 1948, 2181, 2517, 2682
Van91I	CCANNNNNTGG	1 cut(s)	2200
Vha464I	CTTAAG	1 cut(s)	2732
VpaK11BI	GGWCC	2 cut(s)	1450, 2438
XapI	RAATTY	5 cut(s)	208, 320, 1161, 1546, 2464
XceI	RCATGY	3 cut(s)	1002, 1362, 1633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1374
XhoI	CTCGAG	1 cut(s)	2726
XmiI	GTMKAC	4 cut(s)	556, 1605, 2333, 2407
XmnI	GAANNNNTTC	3 cut(s)	924, 1185, 2304
XspI	CTAG	5 cut(s)	960, 1178, 1355, 1688, 2247
Zsp2I	ATGCAT	1 cut(s)	2255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.