MD02G1123500.v1.1

protein At4g08330, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015322
Physical Location & Seq
Forward (+)
10044925 .. 10045317
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1123500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCTTATTCCGATTCGACGATCTACACCTGCACGGAGTGCGGAGCCAACCTGAACCTTTCCGCCGCGAATCTCTACCCTCCGGACTTCTTCTTCGAGGCCGGCAACAAGGGAACGGTGTCTTTTGCGGCGGTCGACACCACCAAGTTCCGGTTCGAGAAGGAGGACAAGATCAGGCCGTTCTTCGAGACCAGAAACTACTGGGGGATCCAACGGACGAGGACGAAGATCAAGTGCAACGGCTGCGGGCGGCTGGTCGGCCACGTCTACGACGATGGGCCCCCCATGACTGACAGTCCCCGCCAGTTTCACATGGGGCCCAGCCAGGTTATCCCTCGAGCTGCTCGGTATAGGTTCAAGACGAAGGCCCTTCGCGTTTCGTCTGGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SelR

Protein Analysis

131

Amino Acids

14.69

Weight (kDa)

9.44

Isoelectric Point (pI)

23.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g08330 PF24046 4 - 127 2.9e-42 At4g08330 beta-tent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015322)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17705
fragaria_vesca FvH4_1g11071
malus_domestica MD02G1123500.v1.1 MD15G1237300.v1.1
prunus_persica Prupe.7G175100_v2.0.a1
pyrus_communis pycom02g09620
rosa_chinensis RchiOBHm_Chr2g0098341
rosa_laevigata RLG00000016792
rosa_multiflora Rmu_sc0003434.1_g000003
rosa_roxburghii Rroxscaffold_2G00144050
rosa_rugosa Rorug02G0072200
rosa_samantha Rh2AG119300 Rh2BG122500 Rh2CG124100 Rh2DG124200
rosa_wichuraiana Rw2G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 38
AccI GTMKAC 2 cut(s) 135, 267
AccII CGCG 2 cut(s) 68, 375
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 8 cut(s) 42, 63, 66, 128, 131, 246, 250, 301
AclWI GGATC 2 cut(s) 202, 215
AcoI YGGCCR 1 cut(s) 259
AdeI CACNNNGTG 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 1 cut(s) 294
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 2 cut(s) 202, 215
Ama87I CYCGRG 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 6 cut(s) 99, 176, 259, 278, 317, 366
ApaI GGGCCC 2 cut(s) 282, 321
ApeKI GCWGC 2 cut(s) 243, 341
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspS9I GGNCC 5 cut(s) 278, 279, 317, 318, 367
AvaI CYCGRG 1 cut(s) 336
BaeGI GKGCMC 2 cut(s) 282, 321
BamHI GGATCC 1 cut(s) 207
BanII GRGCYC 2 cut(s) 282, 321
BbvI GCAGC 2 cut(s) 230, 328
BccI CCATC 1 cut(s) 269
BceAI ACGGC 2 cut(s) 163, 256
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 5 cut(s) 66, 129, 244, 251, 342
BlsI GCNGC 5 cut(s) 67, 130, 245, 252, 343
Bme1390I CCNGG 1 cut(s) 326
BmeRI GACNNNNNGTC 1 cut(s) 294
BmeT110I CYCGRG 1 cut(s) 336
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 5 cut(s) 278, 279, 317, 318, 367
BmiI GGNNCC 6 cut(s) 46, 209, 280, 281, 318, 319
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 211
BmuI ACTGGG 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 182
BsaWI WCCGGW 2 cut(s) 82, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 206, 304
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 326
BseLI CCNNNNNNNGG 1 cut(s) 150
BseNI ACTGG 2 cut(s) 206, 304
BseSI GKGCMC 2 cut(s) 282, 321
BseXI GCAGC 2 cut(s) 230, 328
BseYI CCCAGC 1 cut(s) 320
BsgI GTGCAG 1 cut(s) 16
Bsh1236I CGCG 2 cut(s) 68, 375
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 6 cut(s) 101, 178, 261, 280, 319, 368
BsiEI CGRYCG 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 3 cut(s) 83, 102, 151
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 6 cut(s) 101, 178, 261, 280, 319, 368
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 336
Bsp120I GGGCCC 2 cut(s) 278, 317
Bsp1286I GDGCHC 2 cut(s) 282, 321
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 4 cut(s) 21, 171, 207, 228
BspACI CCGC 8 cut(s) 42, 63, 66, 128, 131, 246, 250, 301
BspANI GGCC 6 cut(s) 101, 178, 261, 280, 319, 368
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 2 cut(s) 68, 375
BspLI GGNNCC 6 cut(s) 46, 209, 280, 281, 318, 319
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 2 cut(s) 202, 215
BspTNI GGTCTC 1 cut(s) 182
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 2 cut(s) 206, 304
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 4 cut(s) 21, 171, 207, 228
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 118, 296
BstAPI GCANNNNNTGC 2 cut(s) 39, 243
BstC8I GCNNGC 2 cut(s) 103, 248
BstDEI CTNAG 1 cut(s) 390
BstFNI CGCG 2 cut(s) 68, 375
BstKTI GATC 4 cut(s) 24, 174, 210, 231
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 4 cut(s) 21, 171, 207, 228
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 2 cut(s) 39, 243
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstSLI GKGCMC 2 cut(s) 282, 321
BstUI CGCG 2 cut(s) 68, 375
BstV1I GCAGC 2 cut(s) 230, 328
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 6 cut(s) 101, 178, 261, 280, 319, 368
BtrI CACGTC 1 cut(s) 265
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 103, 248
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 5 cut(s) 278, 279, 317, 318, 367
CviAII CATG 2 cut(s) 286, 313
DdeI CTNAG 1 cut(s) 390
DpnI GATC 4 cut(s) 23, 173, 209, 230
DpnII GATC 4 cut(s) 21, 171, 207, 228
DraIII CACNNNGTG 1 cut(s) 39
DriI GACNNNNNGTC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 259
Eam1105I GACNNNNNGTC 1 cut(s) 294
EciI GGCGGA 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 282, 321
Eco31I GGTCTC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 336
EcoO109I RGGNCCY 3 cut(s) 279, 317, 367
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 282, 321
FaeI CATG 2 cut(s) 289, 316
FaiI YATR 3 cut(s) 287, 314, 351
FaqI GGGAC 1 cut(s) 282
FatI CATG 2 cut(s) 285, 312
FauI CCCGC 2 cut(s) 239, 308
FblI GTMKAC 2 cut(s) 135, 267
Fnu4HI GCNGC 5 cut(s) 66, 129, 244, 251, 342
FriOI GRGCYC 2 cut(s) 282, 321
Fsp4HI GCNGC 5 cut(s) 66, 129, 244, 251, 342
GluI GCNGC 5 cut(s) 66, 129, 244, 251, 342
GsaI CCCAGC 1 cut(s) 324
HaeIII GGCC 6 cut(s) 101, 178, 261, 280, 319, 368
HapII CCGG 3 cut(s) 83, 102, 151
Hin1II CATG 2 cut(s) 289, 316
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 2 cut(s) 14, 70
HpaII CCGG 3 cut(s) 83, 102, 151
Hpy166II GTNNAC 2 cut(s) 136, 268
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 4 cut(s) 83, 157, 187, 358
Hpy8I GTNNAC 2 cut(s) 136, 268
Hpy99I CGWCG 2 cut(s) 22, 275
HpyAV CCTTC 3 cut(s) 154, 358, 380
HpyCH4III ACNGT 2 cut(s) 118, 296
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 33, 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 243
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 289, 316
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 21, 171, 207, 228
LmnI GCTCC 1 cut(s) 44
Lsp1109I GCAGC 2 cut(s) 230, 328
MaeII ACGT 1 cut(s) 264
MalI GATC 4 cut(s) 23, 173, 209, 230
MboI GATC 4 cut(s) 21, 171, 207, 228
MboII GAAGA 4 cut(s) 82, 85, 175, 238
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 282, 321
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 5 cut(s) 90, 91, 157, 213, 345
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 101
MspI CCGG 3 cut(s) 83, 102, 151
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 2 cut(s) 68, 375
MwoI GCNNNNNNNGC 2 cut(s) 39, 243
NaeI GCCGGC 1 cut(s) 103
NdeII GATC 4 cut(s) 21, 171, 207, 228
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 2 cut(s) 289, 316
NlaIV GGNNCC 6 cut(s) 46, 209, 280, 281, 318, 319
PaeR7I CTCGAG 1 cut(s) 336
PaqCI CACCTGC 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 1 cut(s) 103
PfeI GAWTC 2 cut(s) 14, 70
PkrI GCNGC 5 cut(s) 67, 130, 245, 252, 343
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 6 cut(s) 46, 209, 280, 281, 318, 319
PspOMI GGGCCC 2 cut(s) 278, 317
PspPI GGNCC 5 cut(s) 278, 279, 317, 318, 367
PspXI VCTCGAGB 1 cut(s) 336
PsuI RGATCY 1 cut(s) 207
SalI GTCGAC 1 cut(s) 134
SatI GCNGC 5 cut(s) 66, 129, 244, 251, 342
Sau3AI GATC 4 cut(s) 21, 171, 207, 228
Sau96I GGNCC 5 cut(s) 278, 279, 317, 318, 367
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 282, 321
SetI ASST 7 cut(s) 32, 54, 60, 267, 330, 343, 356
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SsiI CCGC 8 cut(s) 42, 63, 66, 128, 131, 246, 250, 301
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 118, 296
TaiI ACGT 1 cut(s) 267
TaqI TCGA 6 cut(s) 17, 96, 135, 156, 186, 337
TauI GCSGC 3 cut(s) 68, 131, 253
TfiI GAWTC 2 cut(s) 14, 70
TseI GCWGC 2 cut(s) 243, 341
TspGWI ACGGA 2 cut(s) 50, 229
XhoI CTCGAG 1 cut(s) 336
XmiI GTMKAC 2 cut(s) 135, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.